mRNA_H-elongata_contig72782.14954.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig72782.14954.1
Unique NamemRNA_H-elongata_contig72782.14954.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A6H5JAF8_9PHAE (DNA-directed RNA polymerase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAF8_9PHAE)

HSP 1 Score: 189 bits (481), Expect = 1.610e-54
Identity = 92/95 (96.84%), Postives = 95/95 (100.00%), Query Frame = 1 
Query:    1 GKFLEGSSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            GKFLEGS+TIT+GFTHAIS+GNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL
Sbjct:  470 GKFLEGSTTITSGFTHAISSGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 564
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A835Z565_9STRA (DNA-directed RNA polymerase subunit beta n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z565_9STRA)

HSP 1 Score: 172 bits (435), Expect = 1.000e-47
Identity = 83/92 (90.22%), Postives = 86/92 (93.48%), Query Frame = 1 
Query:   10 LEGSSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            L GS  IT GF HAISTGNWV+KRFK+DRQGVTQVLSRLS+IS LGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL
Sbjct:  445 LAGSMNITQGFIHAISTGNWVVKRFKMDRQGVTQVLSRLSYISCLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 536
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A7S3DSN5_9STRA (DNA-directed RNA polymerase (Fragment) n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A7S3DSN5_9STRA)

HSP 1 Score: 155 bits (392), Expect = 1.640e-46
Identity = 72/87 (82.76%), Postives = 83/87 (95.40%), Query Frame = 1 
Query:   25 TITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            TITNG  +AI+TGNWVLKRFK+DR GVTQVLSRLS++S+LGMMTR++SQF+K RKVSGPR+LQPSQWGMLCPADTPEGE+CGLVKNL
Sbjct:   51 TITNGMINAIATGNWVLKRFKMDRAGVTQVLSRLSYMSALGMMTRINSQFEKTRKVSGPRSLQPSQWGMLCPADTPEGEACGLVKNL 137
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A822DRV6_9NEOB (DNA-directed RNA polymerase n=1 Tax=Ranitomeya imitator TaxID=111125 RepID=A0A822DRV6_9NEOB)

HSP 1 Score: 155 bits (393), Expect = 4.850e-46
Identity = 73/86 (84.88%), Postives = 81/86 (94.19%), Query Frame = 1 
Query:   28 ITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            ITNG  +AISTGNW LKRFK+DRQGVTQVLSRLSFIS+LGMMTR+ SQF+K RKVSGPR+LQPSQWGMLCP+DTPEGE+CGLVKNL
Sbjct:   74 ITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSFISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNL 159
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A7S1V6C3_9STRA (DNA-directed RNA polymerase (Fragment) n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1V6C3_9STRA)

HSP 1 Score: 152 bits (385), Expect = 4.810e-45
Identity = 72/87 (82.76%), Postives = 81/87 (93.10%), Query Frame = 1 
Query:   25 TITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            TIT G   AISTGNWVLKRF++DR GVTQVLSRLS++S+LGMMTRV+SQF+K RKVSGPR+LQPSQWGMLCPADTPEGE+CGLVKNL
Sbjct:   19 TITYGMVTAISTGNWVLKRFRMDRAGVTQVLSRLSYVSALGMMTRVNSQFEKTRKVSGPRSLQPSQWGMLCPADTPEGEACGLVKNL 105
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A059LAC5_9CHLO (DNA-directed RNA polymerase (Fragment) n=1 Tax=Helicosporidium sp. ATCC 50920 TaxID=1291522 RepID=A0A059LAC5_9CHLO)

HSP 1 Score: 151 bits (381), Expect = 6.040e-45
Identity = 70/89 (78.65%), Postives = 82/89 (92.13%), Query Frame = 1 
Query:   19 SSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            S T+T G  HA+S+GNW ++RF+++R+GVTQVLSRLSFIS+LGMMTRV SQF+K RKVSGPRALQPSQWGM+CPADTPEGESCGLVKNL
Sbjct:   67 SDTLTYGLEHALSSGNWNIRRFRMERRGVTQVLSRLSFISALGMMTRVTSQFEKTRKVSGPRALQPSQWGMVCPADTPEGESCGLVKNL 155
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A7S0U407_HEMAN (DNA-directed RNA polymerase (Fragment) n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S0U407_HEMAN)

HSP 1 Score: 150 bits (378), Expect = 8.260e-45
Identity = 73/94 (77.66%), Postives = 85/94 (90.43%), Query Frame = 1 
Query:    4 KFLEGSSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            K+L  +S IT+G  +AISTGNW L+RF ++RQGVTQVLSRLSFI++LGM+TRV SQF+K RKVSGPR+LQPSQWGMLCPADTPEGESCGLVKNL
Sbjct:   21 KYLP-ASKITSGLVNAISTGNWNLRRFGMERQGVTQVLSRLSFIAALGMITRVQSQFEKTRKVSGPRSLQPSQWGMLCPADTPEGESCGLVKNL 113
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A7S2WKY6_9STRA (DNA-directed RNA polymerase (Fragment) n=2 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2WKY6_9STRA)

HSP 1 Score: 154 bits (389), Expect = 1.210e-44
Identity = 73/89 (82.02%), Postives = 82/89 (92.13%), Query Frame = 1 
Query:   19 SSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            S TIT G   AISTGNWVLKRF++DR GVTQVLSRLS++S+LGMMTRV+SQF+K RKVSGPR+LQPSQWGMLCPADTPEGE+CGLVKNL
Sbjct:  145 SDTITMGMVMAISTGNWVLKRFRMDRAGVTQVLSRLSYVSALGMMTRVNSQFEKTRKVSGPRSLQPSQWGMLCPADTPEGEACGLVKNL 233
[Close]
BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A7S3PBU7_9STRA (DNA-directed RNA polymerase (Fragment) n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3PBU7_9STRA)

HSP 1 Score: 154 bits (389), Expect = 3.370e-44
Identity = 71/87 (81.61%), Postives = 83/87 (95.40%), Query Frame = 1 
Query:   25 TITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            TITNG  +AI+TGNWVLKRF++DR GVTQVLSRLS++S+LGMMTR++SQF+K RKVSGPR+LQPSQWGMLCPADTPEGE+CGLVKNL
Sbjct:  165 TITNGMINAIATGNWVLKRFRMDRAGVTQVLSRLSYMSALGMMTRINSQFEKTRKVSGPRSLQPSQWGMLCPADTPEGEACGLVKNL 251
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BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Match: A0A482SPL4_9ARCH (DNA-directed RNA polymerase subunit beta n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SPL4_9ARCH)

HSP 1 Score: 160 bits (404), Expect = 1.330e-43
Identity = 76/94 (80.85%), Postives = 86/94 (91.49%), Query Frame = 1 
Query:    4 KFLEGSSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGMMTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNL 285
            KF+ G  TIT GF HAISTG+WVLKRF++DR GVTQVLSRLS+IS+LGMMTRV SQF+K RKVSGPR+LQPSQWGM+CPADTPEGE+CGLVKNL
Sbjct:  193 KFIRGD-TITQGFVHAISTGSWVLKRFRMDRAGVTQVLSRLSYISALGMMTRVSSQFEKTRKVSGPRSLQPSQWGMMCPADTPEGEACGLVKNL 285
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig72782.14954.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JAF8_9PHAE1.610e-5496.84DNA-directed RNA polymerase n=1 Tax=Ectocarpus sp.... [more]
A0A835Z565_9STRA1.000e-4790.22DNA-directed RNA polymerase subunit beta n=1 Tax=T... [more]
A0A7S3DSN5_9STRA1.640e-4682.76DNA-directed RNA polymerase (Fragment) n=1 Tax=Amp... [more]
A0A822DRV6_9NEOB4.850e-4684.88DNA-directed RNA polymerase n=1 Tax=Ranitomeya imi... [more]
A0A7S1V6C3_9STRA4.810e-4582.76DNA-directed RNA polymerase (Fragment) n=1 Tax=Gra... [more]
A0A059LAC5_9CHLO6.040e-4578.65DNA-directed RNA polymerase (Fragment) n=1 Tax=Hel... [more]
A0A7S0U407_HEMAN8.260e-4577.66DNA-directed RNA polymerase (Fragment) n=1 Tax=Hem... [more]
A0A7S2WKY6_9STRA1.210e-4482.02DNA-directed RNA polymerase (Fragment) n=2 Tax=Euc... [more]
A0A7S3PBU7_9STRA3.370e-4481.61DNA-directed RNA polymerase (Fragment) n=1 Tax=Amp... [more]
A0A482SPL4_9ARCH1.330e-4380.85DNA-directed RNA polymerase subunit beta n=1 Tax=a... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig72782contigH-elongata_contig72782:1262..3108 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score161.4
Seed ortholog evalue2.1e-37
Seed eggNOG ortholog44056.XP_009041655.1
Preferred namePOLR3B
KEGG rclassRC02795
KEGG koko:K03010,ko:K03021
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko04623,ko05016,ko05169,map00230,map00240,map01100,map03020,map04623,map05016,map05169
KEGG ModuleM00180,M00181
GOsGO:0000428,GO:0001817,GO:0001819,GO:0002682,GO:0002684,GO:0003674,GO:0003824,GO:0003899,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005666,GO:0005730,GO:0005736,GO:0005737,GO:0005829,GO:0006139,GO:0006351,GO:0006360,GO:0006383,GO:0006399,GO:0006725,GO:0006807,GO:0007275,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009304,GO:0009553,GO:0009561,GO:0009987,GO:0010467,GO:0016070,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019438,GO:0030880,GO:0031347,GO:0031349,GO:0031974,GO:0031981,GO:0032479,GO:0032481,GO:0032501,GO:0032502,GO:0032648,GO:0032728,GO:0032774,GO:0032991,GO:0034062,GO:0034641,GO:0034645,GO:0034654,GO:0034660,GO:0042797,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044452,GO:0044464,GO:0045088,GO:0045089,GO:0046483,GO:0048229,GO:0048518,GO:0048583,GO:0048584,GO:0048856,GO:0050776,GO:0050778,GO:0050789,GO:0051239,GO:0051240,GO:0055029,GO:0061695,GO:0065007,GO:0070013,GO:0071704,GO:0080134,GO:0090304,GO:0097659,GO:0097747,GO:0098781,GO:0140098,GO:1901360,GO:1901362,GO:1901576,GO:1902494,GO:1990234
EggNOG free text desc.DNA-directed 5'-3' RNA polymerase activity
EggNOG OGsCOG0085@1,KOG0215@2759
EC2.7.7.6
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01611,ko00000,ko00001,ko00002,ko01000,ko03021,ko03400
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionDNA-directed RNA polymerase, subunit 2
Ec32 orthologEc-00_002160.1
Exons2
Model size288
Cds size288
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931129.923817-CDS-H-elongata_contig72782:1261..13721622931129.923817-CDS-H-elongata_contig72782:1261..1372Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72782 1262..1372 +
1691679710.3777833-CDS-H-elongata_contig72782:1261..13721691679710.3777833-CDS-H-elongata_contig72782:1261..1372Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72782 1262..1372 +
1622931129.9400067-CDS-H-elongata_contig72782:2931..31081622931129.9400067-CDS-H-elongata_contig72782:2931..3108Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72782 2932..3108 +
1691679710.3887641-CDS-H-elongata_contig72782:2931..31081691679710.3887641-CDS-H-elongata_contig72782:2931..3108Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72782 2932..3108 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig72782.14954.1prot_H-elongata_contig72782.14954.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig72782 1262..3108 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig72782.14954.1

>prot_H-elongata_contig72782.14954.1 ID=prot_H-elongata_contig72782.14954.1|Name=mRNA_H-elongata_contig72782.14954.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=96bp
GKFLEGSSTITNGFTHAISTGNWVLKRFKVDRQGVTQVLSRLSFISSLGM
MTRVDSQFQKERKVSGPRALQPSQWGMLCPADTPEGESCGLVKNLG
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mRNA from alignment at H-elongata_contig72782:1262..3108+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig72782.14954.1 ID=mRNA_H-elongata_contig72782.14954.1|Name=mRNA_H-elongata_contig72782.14954.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1847bp|location=Sequence derived from alignment at H-elongata_contig72782:1262..3108+ (Himanthalia elongata Himel1 dioecious)
GGGAAGTTTTTGGAGGGGTCATCCACTATCACGAATGGTTTCACGCACGC
GATTTCGACGGGGAACTGGGTTCTCAAGCGTTTCAAGGTCGACAGGCAGG
GAGTCACACAGGTGGGAGGAGCTTTCGAGGGGAGGCTTCTGTGTGGGTCT
CAAATATGTATCTAGGTGTTAGCATTGTCTTGGGGGGGGATCGTCGCGGC
CCCTGTTGGCACCCCGGCCTGTTACCGAGCGGGTTTCGGAAAAGGCGGGT
AAATATTACTGTTCGGTGACACGTTATGCCTGTCTTCGCCTCTTGATGAT
GAGCGACTTTTGAGGGTGATTTTGGAGTTAAAATCCACACCCTTCTTCCA
TTTATTTTGGGTTTCGATATTCTTTGTTGCGTTTCTCATCTGACCACAAG
CAAGCAGGATTGGCAACCAATTGGTGGGACGGTCGGCTGTTAGTGTAATA
ATCAATGAATCAGTCAGTACACTTCGTTAAACATACGAAGTACTATACTA
TATATAGCATATTATTAGTTAAACTAGTGCCTTCGTGATGAGCGACATTT
GGGGGTAATTTTGGGAATGTTCTCTCTCTCCCCCCCTCCCCCTCCCAACT
AAAATTTGTTGTACATATGTAGAAAGCCGATAGTGGAACACAAGGCAATA
ATAATAACCTTAATTTTGCTTGAGATATACGGGTAAAAGTCTCCCTGGCT
TCTTATCATCTCCGAAAGACGGGGAGGTTTGCCTCCCTTAAGAAGGGGTC
TGTATAAACAAGGGAAACCCGTCTCTATATTGACGGTTGCTTTATTGTGC
CATGTACGGTACTAGTTAAAAATTCGTCTTCGTCTTGACGTTGCCGGGAG
GTGGGACGATCGCCGTGGCGTCTGAAGATGATGCCTCATTACGCTGTAGA
GATCCCACTCTTGAACGGTGTAACGTTGAAAACCACCTTGCAAGTAGCAT
TGCAGGGGGGGGCATACTTAAGTTGTATCTACTTTCGCTGTTTGGCCATT
AACCACGCGTTCTCGGGAGGAACTAATTATCTTGTAGTTTAAAGGCTTTT
TTGTTGCTGTTCAAAAATAAGTGGCCGGTATGAGTTTTCATATGTTTTGC
AGGCTTTTTGTCTAGGCTTGTTTGTAAAGGACTTCAAAATATACAAATAA
TTAGGGCGAGTATTACGGGGTTACAAAAAAAAAAATCCAGCTGATTTAAA
TTTAAATCGAGTTCTGATTAGTCGAAAGCTAAATAACGCCTAGCCTTCGT
CCAATCAGAGTCCGATTTAATCTTAAATCGGCTGGAATTTAAATATTTGT
AACCCCTTTAATACCCGCCCTCACCTATCCTATTATATCTACCGGTACCA
GCTGCATCTTTTATTCTTCTCGGAGAAGAAGCTTTCGGCGACCTTTCCGA
CGATCGTTCCGACGATCGTTTCGACGACTGCTTGTGACGTCGCGATGTGG
AACGCCCCTCTCTTGGTTGCGTTTTTGTATCATGCTAGTCTATTAATCGC
AGCCCTAAAGGCCTTCGCGTGTCTTAATTTCTCACAGTACGCACTTAGTA
TGGCCAGTTCGAGCTGTAGTAGTAGCGGTATTTGCATCTTCTGTTTTTGA
TTTTTTTTACTTTCTGTAAATAAATGCCCCTCCTTTCCAAACCGCCCAAA
CCCCCTTCCTTTTTATTTAGGTGCTCTCGAGGCTGAGCTTTATCTCCTCT
CTGGGGATGATGACCAGAGTAGACTCGCAGTTTCAGAAGGAACGCAAGGT
CTCGGGACCCAGGGCGCTGCAACCCAGCCAATGGGGCATGCTCTGTCCGG
CTGACACCCCAGAGGGAGAGAGCTGCGGTCTGGTCAAAAACCTTGGT
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Coding sequence (CDS) from alignment at H-elongata_contig72782:1262..3108+

>mRNA_H-elongata_contig72782.14954.1 ID=mRNA_H-elongata_contig72782.14954.1|Name=mRNA_H-elongata_contig72782.14954.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=576bp|location=Sequence derived from alignment at H-elongata_contig72782:1262..3108+ (Himanthalia elongata Himel1 dioecious)
GGGAAGTTTTTGGAGGGGTCATCCACTATCACGAATGGTTTCACGCACGC
GATTTCGACGGGGAACTGGGTTCTCAAGCGTTTCAAGGTCGACAGGCAGG
GAGTCACACAGGGGAAGTTTTTGGAGGGGTCATCCACTATCACGAATGGT
TTCACGCACGCGATTTCGACGGGGAACTGGGTTCTCAAGCGTTTCAAGGT
CGACAGGCAGGGAGTCACACAGGTGCTCTCGAGGCTGAGCTTTATCTCCT
CTCTGGGGATGATGACCAGAGTAGACTCGCAGTTTCAGAAGGAACGCAAG
GTCTCGGGACCCAGGGCGCTGCAACCCAGCCAATGGGGCATGCTCTGTCC
GGCTGACACCCCAGAGGGAGAGAGCTGCGGTCTGGTCAAAAACCTTGGTG
TGCTCTCGAGGCTGAGCTTTATCTCCTCTCTGGGGATGATGACCAGAGTA
GACTCGCAGTTTCAGAAGGAACGCAAGGTCTCGGGACCCAGGGCGCTGCA
ACCCAGCCAATGGGGCATGCTCTGTCCGGCTGACACCCCAGAGGGAGAGA
GCTGCGGTCTGGTCAAAAACCTTGGT
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