mRNA_H-elongata_contig61029.13651.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig61029.13651.1
Unique NamemRNA_H-elongata_contig61029.13651.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: D7FQ94_ECTSI (Small nucleolar ribonucleoprotein U3 component n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FQ94_ECTSI)

HSP 1 Score: 188 bits (478), Expect = 4.700e-59
Identity = 95/106 (89.62%), Postives = 102/106 (96.23%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLKYHEQKLLKKVDFLQWKSD+NIREIQVLRRYLVQNRDDY KYNKIVGLITKLTARLKTLD SDEFRIKMTEQ+L+KLF+MG+IN KKSLAKTE A+VS+FCR
Sbjct:    1 MRKLKYHEQKLLKKVDFLQWKSDQNIREIQVLRRYLVQNRDDYVKYNKIVGLITKLTARLKTLDASDEFRIKMTEQLLEKLFAMGIINTKKSLAKTETASVSSFCR 106
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A6S9JYI8_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6S9JYI8_HETAK)

HSP 1 Score: 156 bits (394), Expect = 2.660e-46
Identity = 76/106 (71.70%), Postives = 93/106 (87.74%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLK+HEQKLLKKVDFLQWKSD+NIREI+++RRYL+Q+R+DYTKYNKI G + KL A+L+ L P+D+FRIKMTE +L+KLF+MGVIN+KKSL   E   VSAFCR
Sbjct:    1 MRKLKHHEQKLLKKVDFLQWKSDQNIREIKIIRRYLLQDREDYTKYNKICGHMWKLVAKLRALPPTDQFRIKMTEDLLEKLFNMGVINSKKSLQLVEKVNVSAFCR 106
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A836CN51_9STRA (Small nucleolar ribonucleoprotein U3 component n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CN51_9STRA)

HSP 1 Score: 151 bits (382), Expect = 1.820e-44
Identity = 75/106 (70.75%), Postives = 90/106 (84.91%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLKYHEQKLLKKVDFLQWKSD+NIREI+VLRRY +Q+R+DY KYN++VG +T L A+LKTL  +D FR+KMT Q+L KL S+GVI+ +KSL K E  TVSAFCR
Sbjct:    1 MRKLKYHEQKLLKKVDFLQWKSDQNIREIKVLRRYHIQDREDYVKYNRLVGHVTSLAAKLKTLSSTDPFRVKMTSQLLSKLQSIGVIDTEKSLIKAEQLTVSAFCR 106
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: W7TJ95_9STRA (Ribosomal protein S4/S9 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TJ95_9STRA)

HSP 1 Score: 149 bits (377), Expect = 3.000e-44
Identity = 73/106 (68.87%), Postives = 90/106 (84.91%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MR+L++HEQKLLKKVDFLQWKSD+NIREI++LRRY +Q+R+DY KYNKI G ITKLTA+LK L P DEFRIKMT+ +L KL++MG+I  KKSL++ E    SAFCR
Sbjct:    1 MRQLRHHEQKLLKKVDFLQWKSDQNIREIKILRRYHIQDREDYVKYNKICGHITKLTAKLKDLKPEDEFRIKMTDDLLSKLYNMGLIPTKKSLSQCETLAASAFCR 106
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: W7TBD6_9STRA (Ribosomal protein S4/S9 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=W7TBD6_9STRA)

HSP 1 Score: 149 bits (377), Expect = 1.010e-43
Identity = 73/106 (68.87%), Postives = 90/106 (84.91%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MR+L++HEQKLLKKVDFLQWKSD+NIREI++LRRY +Q+R+DY KYNKI G ITKLTA+LK L P DEFRIKMT+ +L KL++MG+I  KKSL++ E    SAFCR
Sbjct:    1 MRQLRHHEQKLLKKVDFLQWKSDQNIREIKILRRYHIQDREDYVKYNKICGHITKLTAKLKDLKPEDEFRIKMTDDLLSKLYNMGLIPTKKSLSQCETLAASAFCR 106
[Close]
BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A060S5L8_PLARE (U3 small nucleolar ribonucleoprotein IMP3, putative n=26 Tax=Plasmodium TaxID=5820 RepID=A0A060S5L8_PLARE)

HSP 1 Score: 149 bits (375), Expect = 2.030e-43
Identity = 71/106 (66.98%), Postives = 89/106 (83.96%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLKYHEQKLLKKV+F  WK   N+RE++VLR+Y++QNR+DYTKYNKI G ITKL ++L+ L  +DEFRIKMTE++LDKL+ MG+IN K SLA+ E  TVS+FCR
Sbjct:    1 MRKLKYHEQKLLKKVNFYDWKRTNNVREVKVLRKYVIQNREDYTKYNKICGYITKLVSKLRLLPENDEFRIKMTEELLDKLYDMGLINYKSSLAECEKITVSSFCR 106
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BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A077TNV8_PLACH (U3 small nucleolar ribonucleoprotein protein IMP3, putative n=13 Tax=Plasmodium (Vinckeia) TaxID=418101 RepID=A0A077TNV8_PLACH)

HSP 1 Score: 148 bits (374), Expect = 2.880e-43
Identity = 70/106 (66.04%), Postives = 90/106 (84.91%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLKYHEQKLLKKV+F  WK  +N+RE++VLRRY++QNR+DYTKYNKI G ITKL ++L+ L  +DEFRIKMT+++LDKL+ MG+IN K SLA+ E  +VS+FCR
Sbjct:    1 MRKLKYHEQKLLKKVNFYDWKRTQNVREVKVLRRYVIQNREDYTKYNKICGYITKLVSKLRLLPENDEFRIKMTDELLDKLYEMGLINCKSSLAECEKLSVSSFCR 106
[Close]
BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A250X5I3_9CHLO (Uncharacterized protein n=1 Tax=Chlamydomonas eustigma TaxID=1157962 RepID=A0A250X5I3_9CHLO)

HSP 1 Score: 147 bits (371), Expect = 7.990e-43
Identity = 72/106 (67.92%), Postives = 91/106 (85.85%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MR+LK+HE+KLLKKVDFL+WK++ N+RE+QV+RRY +Q+RDDY KYNKIVGLITKLT+ LK LD  D  RI++T+Q+L+KLFSMGV+  KKSL +TE   VSAFCR
Sbjct:    1 MRQLKFHEKKLLKKVDFLKWKNEDNLRELQVMRRYHIQDRDDYKKYNKIVGLITKLTSVLKQLDAKDTARIELTDQLLEKLFSMGVVPTKKSLVQTEKLAVSAFCR 106
[Close]
BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: A0A8C9GZ43_9PRIM (Ribosomal_S4 domain-containing protein n=3 Tax=Eukaryota TaxID=2759 RepID=A0A8C9GZ43_9PRIM)

HSP 1 Score: 147 bits (371), Expect = 8.230e-43
Identity = 70/106 (66.04%), Postives = 88/106 (83.02%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MRKLKYHEQKLLKKV+F  WK  +N+RE++VLRRYL+QNR+DY KYNKI G ITKL ++L+ L  +DEFRIKMT ++LDKL+ MG+IN K SLA+ E  +VS+FCR
Sbjct:    1 MRKLKYHEQKLLKKVNFYDWKRTKNVREVKVLRRYLIQNREDYAKYNKICGYITKLVSKLRLLPENDEFRIKMTSELLDKLYEMGIINNKSSLAECERISVSSFCR 106
[Close]
BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Match: D8UBE0_VOLCA (Uncharacterized protein n=2 Tax=Volvox TaxID=3066 RepID=D8UBE0_VOLCA)

HSP 1 Score: 146 bits (369), Expect = 1.610e-42
Identity = 70/106 (66.04%), Postives = 89/106 (83.96%), Query Frame = 1 
Query:    4 MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIVGLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENATVSAFCR 321
            MR+LKYHEQKLLKKVDFL+WKSD N+RE+QVLRRY +Q+RDDY KYNKI G+ITKLT+ LK LDP D  RI++T+Q+L+KL++MGV+  KKSL + +    +AFCR
Sbjct:    1 MRQLKYHEQKLLKKVDFLRWKSDDNVRELQVLRRYHIQDRDDYKKYNKICGMITKLTSMLKRLDPKDATRIELTDQLLEKLYNMGVLPTKKSLVQCDKLATAAFCR 106
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig61029.13651.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FQ94_ECTSI4.700e-5989.62Small nucleolar ribonucleoprotein U3 component n=2... [more]
A0A6S9JYI8_HETAK2.660e-4671.70Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A836CN51_9STRA1.820e-4470.75Small nucleolar ribonucleoprotein U3 component n=1... [more]
W7TJ95_9STRA3.000e-4468.87Ribosomal protein S4/S9 n=1 Tax=Nannochloropsis ga... [more]
W7TBD6_9STRA1.010e-4368.87Ribosomal protein S4/S9 n=3 Tax=Monodopsidaceae Ta... [more]
A0A060S5L8_PLARE2.030e-4366.98U3 small nucleolar ribonucleoprotein IMP3, putativ... [more]
A0A077TNV8_PLACH2.880e-4366.04U3 small nucleolar ribonucleoprotein protein IMP3,... [more]
A0A250X5I3_9CHLO7.990e-4367.92Uncharacterized protein n=1 Tax=Chlamydomonas eust... [more]
A0A8C9GZ43_9PRIM8.230e-4366.04Ribosomal_S4 domain-containing protein n=3 Tax=Euk... [more]
D8UBE0_VOLCA1.610e-4266.04Uncharacterized protein n=2 Tax=Volvox TaxID=3066 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig61029contigH-elongata_contig61029:716..2081 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score191.0
Seed ortholog evalue2.9e-46
Seed eggNOG ortholog2880.D7FQ94
Preferred nameIMP3
KEGG koko:K14560
KEGG Pathwayko03008,map03008
GOsGO:0003674,GO:0003676,GO:0003723,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005730,GO:0005732,GO:0006139,GO:0006364,GO:0006396,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009987,GO:0010467,GO:0016070,GO:0016072,GO:0022613,GO:0030515,GO:0030532,GO:0030684,GO:0030686,GO:0031974,GO:0031981,GO:0032040,GO:0032991,GO:0034457,GO:0034470,GO:0034641,GO:0034660,GO:0042254,GO:0042274,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044452,GO:0044464,GO:0046483,GO:0070013,GO:0071704,GO:0071840,GO:0090304,GO:0097159,GO:0120114,GO:1901360,GO:1901363,GO:1990904
EggNOG free text desc.snoRNA binding
EggNOG OGsCOG0522@1,KOG4655@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03009
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionRibosomal protein S4/S9, N-terminal
Ec32 orthologEc-01_006990.1
Exons3
Model size342
Cds size339
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig61029.13651.1prot_H-elongata_contig61029.13651.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig61029 716..2078 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930985.4909148-CDS-H-elongata_contig61029:715..8711622930985.4909148-CDS-H-elongata_contig61029:715..871Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 716..871 -
1691679651.2403712-CDS-H-elongata_contig61029:715..8711691679651.2403712-CDS-H-elongata_contig61029:715..871Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 716..871 -
1622930985.5129604-CDS-H-elongata_contig61029:1308..14011622930985.5129604-CDS-H-elongata_contig61029:1308..1401Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 1309..1401 -
1691679651.2536294-CDS-H-elongata_contig61029:1308..14011691679651.2536294-CDS-H-elongata_contig61029:1308..1401Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 1309..1401 -
1622930985.529067-CDS-H-elongata_contig61029:1988..20781622930985.529067-CDS-H-elongata_contig61029:1988..2078Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 1989..2078 -
1691679651.268536-CDS-H-elongata_contig61029:1988..20781691679651.268536-CDS-H-elongata_contig61029:1988..2078Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig61029 1989..2078 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930985.5481105-UTR-H-elongata_contig61029:2078..20811622930985.5481105-UTR-H-elongata_contig61029:2078..2081Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig61029 2079..2081 -
1691679651.27666-UTR-H-elongata_contig61029:2078..20811691679651.27666-UTR-H-elongata_contig61029:2078..2081Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig61029 2079..2081 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig61029.13651.1

>prot_H-elongata_contig61029.13651.1 ID=prot_H-elongata_contig61029.13651.1|Name=mRNA_H-elongata_contig61029.13651.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=113bp
MRKLKYHEQKLLKKVDFLQWKSDRNIREIQVLRRYLVQNRDDYTKYNKIV
GLITKLTARLKTLDPSDEFRIKMTEQMLDKLFSMGVINAKKSLAKTENAT
VSAFCRCVLLIS*
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mRNA from alignment at H-elongata_contig61029:716..2081-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig61029.13651.1 ID=mRNA_H-elongata_contig61029.13651.1|Name=mRNA_H-elongata_contig61029.13651.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1366bp|location=Sequence derived from alignment at H-elongata_contig61029:716..2081- (Himanthalia elongata Himel1 dioecious)
ATCATGAGAAAGCTCAAGTACCATGAGCAGAAGCTTCTGAAGAAGGTCGA
CTTCTTGCAATGGAAGAGTGACCGAAACATCAGAGAAATACAGGTAATAA
TAATACGGTAGATTAGCTTGTTGTAGCCGCACGGTCCAGGCGATGGGAAG
GATGATTTTAAATTAGGAGAGGATCCATCCATTCGACTCAGCGTGGTGGC
GAGGGAGAATATACTCTTGCGGTGTGTACACTTGCTGAAGGGAACGTTTA
TTAAGAGATATCGGTCCCCATCGCTATAAGAGCTATTTGGGGCTGGTGGG
AGCTTTGTGCTCCGAATCATTTTTTATGTTAAAAAAAAAACTAACCCCAA
CGCAGTAGAGCCACCCTTCAGCCTTATAACAAAAAACATATCACGAGCTA
TATAATACATTGTACTGTCTGTAGAAGAGCTCCTAATATCGCTAGTATCG
TGTGATGTGGATAGCAAATGATTAGCTGAAGACACCCACAAAAGACCTCA
AGAAAACAGCATCAAAGTTGGACCCCGAGTGGCGAGAGCAGAGGCAAGAG
CCCCAAAAGCGAGGGGTGATGAAGTGCCCCAAAACCTGTCAAATTAGTGC
AAATCTTCCGATCTTCCGAGGCCCCTCGTCTCTATTTTTCGCCTCCTCTA
TAGAACGCAATCGACACTATGCTTCGTCAGGTCCTCCGCAGATACCTTGT
ACAAAATCGAGACGACTACACGAAGTATAACAAGATCGTGGGGCTGATCA
CGAAGCTCACTGCGAGGCTCAAGGTATTTGAACAACCGAATATCATCTCG
CATTATTAATAGAAACTCTTCTGCTTCGGAAGCTTCAAATCCCTCCTTAT
TGGCCCACGTGCCGGAAACCCGCGTGTACAGCGCTTGTCCATATTTTTGA
GTTGACCGTTTTGAAAGACATTTCCCCGCGGAGTGGGCACGGAAGCAACG
GGGCGTACTGTGTTAGCAACAGCAGTAATACAATACTCGAAACTCACGCT
GCTGAAGGCCGCTCTCTGGATTGGCGAGAGCCAACCGGTAGATTATTTTT
CATAGCTCTAGTGACCTTGCGTGTAGGCTAAAATGCTCGGCTCTTCGCCA
CGTCTTAAGCCGGAAGATTCGTGTCTTCTTGGGTAAAGCCCCCTGCACCA
TGAGCCGACTCACTGATATGCCTCCTGCGTAATCGCGTGGCGACCGCTTA
TGTTCGACAGACTTTAGACCCCAGCGATGAGTTCCGGATAAAGATGACGG
AGCAGATGCTTGACAAGCTTTTCTCGATGGGGGTAATCAACGCGAAGAAG
AGTCTGGCCAAGACCGAGAATGCCACCGTGTCCGCCTTCTGCAGGTGTGT
GTTACTGATATCTTGA
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Coding sequence (CDS) from alignment at H-elongata_contig61029:716..2081-

>mRNA_H-elongata_contig61029.13651.1 ID=mRNA_H-elongata_contig61029.13651.1|Name=mRNA_H-elongata_contig61029.13651.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=678bp|location=Sequence derived from alignment at H-elongata_contig61029:716..2081- (Himanthalia elongata Himel1 dioecious)
ATGAGAAAGCTCAAGTACCATGAGCAGAAGCTTCTGAAGAAGGTCGACTT
CTTGCAATGGAAGAGTGACCGAAACATCAGAGAAATACAGATGAGAAAGC
TCAAGTACCATGAGCAGAAGCTTCTGAAGAAGGTCGACTTCTTGCAATGG
AAGAGTGACCGAAACATCAGAGAAATACAGGTCCTCCGCAGATACCTTGT
ACAAAATCGAGACGACTACACGAAGTATAACAAGATCGTGGGGCTGATCA
CGAAGCTCACTGCGAGGCTCAAGGTCCTCCGCAGATACCTTGTACAAAAT
CGAGACGACTACACGAAGTATAACAAGATCGTGGGGCTGATCACGAAGCT
CACTGCGAGGCTCAAGACTTTAGACCCCAGCGATGAGTTCCGGATAAAGA
TGACGGAGCAGATGCTTGACAAGCTTTTCTCGATGGGGGTAATCAACGCG
AAGAAGAGTCTGGCCAAGACCGAGAATGCCACCGTGTCCGCCTTCTGCAG
GTGTGTGTTACTGATATCTTGAACTTTAGACCCCAGCGATGAGTTCCGGA
TAAAGATGACGGAGCAGATGCTTGACAAGCTTTTCTCGATGGGGGTAATC
AACGCGAAGAAGAGTCTGGCCAAGACCGAGAATGCCACCGTGTCCGCCTT
CTGCAGGTGTGTGTTACTGATATCTTGA
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