mRNA_H-elongata_contig55501.12966.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig55501.12966.1
Unique NamemRNA_H-elongata_contig55501.12966.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: D7G7U9_ECTSI (E3 ubiquitin protein ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7U9_ECTSI)

HSP 1 Score: 119 bits (298), Expect = 6.320e-30
Identity = 52/58 (89.66%), Postives = 54/58 (93.10%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +MIKCSVCQDKKKNRVITKCFHMFCDGCLE+SIKSRNRKCPAC K Y  DDVHELWLT
Sbjct:  833 KMIKCSVCQDKKKNRVITKCFHMFCDGCLEKSIKSRNRKCPACGKVYAPDDVHELWLT 890
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A662Y869_9STRA (E3 ubiquitin protein ligase n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662Y869_9STRA)

HSP 1 Score: 92.4 bits (228), Expect = 1.830e-20
Identity = 35/58 (60.34%), Postives = 48/58 (82.76%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++KCSVCQD++K+ +ITKCFHMFC  C++ ++KSRNRKCP C K +G DDV  +WLT
Sbjct:  811 KLVKCSVCQDRRKDVIITKCFHMFCKECVDNNLKSRNRKCPTCKKMFGQDDVKAVWLT 868
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A7S2Y421_9STRA (E3 ubiquitin protein ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2Y421_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 1.870e-20
Identity = 34/57 (59.65%), Postives = 48/57 (84.21%), Query Frame = 1 
Query:    4 MIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            M++CSVCQD++KN V+TKCFHMFC  C+++++K R+RKCPAC + +G DDV  +WLT
Sbjct:  269 MLRCSVCQDRRKNTVLTKCFHMFCRECVQENLKIRSRKCPACGRMFGQDDVRTIWLT 325
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: K3WQG6_GLOUD (E3 ubiquitin protein ligase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WQG6_GLOUD)

HSP 1 Score: 89.4 bits (220), Expect = 2.210e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++KN +I+KCFHMFC  C++ ++KSRNRKCP C K +G DDV  +W T
Sbjct:  774 KLVNCSVCQDQRKNVLISKCFHMFCKDCIDSNLKSRNRKCPTCKKMFGQDDVKSVWFT 831
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: G4YGB4_PHYSP (E3 ubiquitin protein ligase n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4YGB4_PHYSP)

HSP 1 Score: 89.0 bits (219), Expect = 3.020e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++K+ +I+KCFHMFC  C+E ++KSRNRKCP C K +G DDV  +W T
Sbjct:  754 KLVNCSVCQDRRKDVIISKCFHMFCKECIENNLKSRNRKCPTCKKMFGHDDVKSVWFT 811
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: W2QVV9_PHYPN (E3 ubiquitin protein ligase n=9 Tax=Phytophthora TaxID=4783 RepID=W2QVV9_PHYPN)

HSP 1 Score: 89.0 bits (219), Expect = 3.020e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++K+ +I+KCFHMFC  C+E ++KSRNRKCP C K +G DDV  +W T
Sbjct:  780 KLVNCSVCQDRRKDVIISKCFHMFCKECIENNLKSRNRKCPTCKKMFGHDDVKSVWFT 837
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A484E9R0_BRELC (E3 ubiquitin protein ligase n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484E9R0_BRELC)

HSP 1 Score: 89.0 bits (219), Expect = 3.020e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++K+ +ITKCFH+FC  C+E ++KSRNRKCP C K +G DDV  +W T
Sbjct:  800 KLVNCSVCQDRRKDVIITKCFHIFCKECIENNLKSRNRKCPTCKKMFGQDDVKTVWFT 857
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A5D6XN03_9STRA (E3 ubiquitin protein ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XN03_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 3.020e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++KCSVCQD++KN ++ KCFHMFC  C++ ++KSRNRKCP C K +G DDV  +W T
Sbjct:  803 KLVKCSVCQDQRKNVLLAKCFHMFCRDCVDSNLKSRNRKCPTCKKMFGQDDVKAVWFT 860
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A2P4YBG3_9STRA (E3 ubiquitin protein ligase (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4YBG3_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 3.680e-19
Identity = 32/58 (55.17%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++K+ +I+KCFHMFC  C++ ++KSRNRKCP C K +G DDV  +W T
Sbjct:  267 KLVNCSVCQDRRKDVIISKCFHMFCKECIDNNLKSRNRKCPTCKKMFGHDDVKTVWFT 324
[Close]
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Match: A0A329T6M6_9STRA (E3 ubiquitin protein ligase n=1 Tax=Phytophthora cactorum TaxID=29920 RepID=A0A329T6M6_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 4.120e-19
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 1 
Query:    1 QMIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDDVHELWLT 174
            +++ CSVCQD++K+ +I+KCFHMFC  C+E ++KSRNRKCP C K +G DDV  +W T
Sbjct:  780 KLVNCSVCQDRRKDVIISKCFHMFCKECIENNLKSRNRKCPTCKKMFGHDDVKTVWFT 837
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig55501.12966.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G7U9_ECTSI6.320e-3089.66E3 ubiquitin protein ligase n=1 Tax=Ectocarpus sil... [more]
A0A662Y869_9STRA1.830e-2060.34E3 ubiquitin protein ligase n=2 Tax=Nothophytophth... [more]
A0A7S2Y421_9STRA1.870e-2059.65E3 ubiquitin protein ligase n=1 Tax=Fibrocapsa jap... [more]
K3WQG6_GLOUD2.210e-1956.90E3 ubiquitin protein ligase n=1 Tax=Globisporangiu... [more]
G4YGB4_PHYSP3.020e-1956.90E3 ubiquitin protein ligase n=1 Tax=Phytophthora s... [more]
W2QVV9_PHYPN3.020e-1956.90E3 ubiquitin protein ligase n=9 Tax=Phytophthora T... [more]
A0A484E9R0_BRELC3.020e-1956.90E3 ubiquitin protein ligase n=1 Tax=Bremia lactuca... [more]
A0A5D6XN03_9STRA3.020e-1956.90E3 ubiquitin protein ligase n=1 Tax=Pythium brassi... [more]
A0A2P4YBG3_9STRA3.680e-1955.17E3 ubiquitin protein ligase (Fragment) n=1 Tax=Phy... [more]
A0A329T6M6_9STRA4.120e-1956.90E3 ubiquitin protein ligase n=1 Tax=Phytophthora c... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig55501contigH-elongata_contig55501:897..1677 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score117.9
Seed ortholog evalue1.6e-24
Seed eggNOG ortholog2880.D7G7U9
KEGG koko:K10696
GOsGO:0000075,GO:0000077,GO:0000151,GO:0000152,GO:0000209,GO:0000228,GO:0000724,GO:0000725,GO:0000726,GO:0000729,GO:0000775,GO:0000785,GO:0000790,GO:0000792,GO:0000793,GO:0000794,GO:0000795,GO:0000800,GO:0000930,GO:0000931,GO:0001067,GO:0001817,GO:0001819,GO:0003674,GO:0003676,GO:0003677,GO:0003712,GO:0003713,GO:0003723,GO:0003824,GO:0004842,GO:0005102,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005720,GO:0005721,GO:0005737,GO:0005815,GO:0005856,GO:0005886,GO:0006139,GO:0006259,GO:0006260,GO:0006281,GO:0006282,GO:0006301,GO:0006302,GO:0006303,GO:0006310,GO:0006323,GO:0006325,GO:0006333,GO:0006334,GO:0006336,GO:0006342,GO:0006355,GO:0006357,GO:0006359,GO:0006464,GO:0006508,GO:0006725,GO:0006807,GO:0006915,GO:0006950,GO:0006974,GO:0006978,GO:0006996,GO:0007059,GO:0007154,GO:0007165,GO:0007275,GO:0007549,GO:0008092,GO:0008134,GO:0008150,GO:0008152,GO:0008219,GO:0008270,GO:0008274,GO:0008630,GO:0009048,GO:0009058,GO:0009059,GO:0009314,GO:0009628,GO:0009719,GO:0009725,GO:0009755,GO:0009790,GO:0009792,GO:0009889,GO:0009890,GO:0009891,GO:0009892,GO:0009893,GO:0009966,GO:0009968,GO:0009987,GO:0010033,GO:0010212,GO:0010243,GO:0010468,GO:0010556,GO:0010557,GO:0010558,GO:0010564,GO:0010565,GO:0010574,GO:0010575,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0010638,GO:0010639,GO:0010646,GO:0010648,GO:0010824,GO:0010826,GO:0010941,GO:0010948,GO:0012501,GO:0014070,GO:0015630,GO:0015631,GO:0016020,GO:0016043,GO:0016458,GO:0016567,GO:0016569,GO:0016570,GO:0016574,GO:0016579,GO:0016740,GO:0019216,GO:0019217,GO:0019219,GO:0019222,GO:0019538,GO:0019787,GO:0019899,GO:0022603,GO:0022607,GO:0023051,GO:0023052,GO:0023057,GO:0030330,GO:0030518,GO:0030521,GO:0030522,GO:0031056,GO:0031057,GO:0031058,GO:0031060,GO:0031061,GO:0031062,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031328,GO:0031371,GO:0031396,GO:0031398,GO:0031399,GO:0031400,GO:0031401,GO:0031436,GO:0031497,GO:0031570,GO:0031572,GO:0031618,GO:0031625,GO:0031974,GO:0031981,GO:0032268,GO:0032269,GO:0032270,GO:0032446,GO:0032501,GO:0032502,GO:0032870,GO:0032886,GO:0032991,GO:0033043,GO:0033044,GO:0033143,GO:0033144,GO:0033146,GO:0033147,GO:0033503,GO:0033523,GO:0033554,GO:0033993,GO:0034097,GO:0034612,GO:0034622,GO:0034641,GO:0034645,GO:0034724,GO:0034728,GO:0035065,GO:0035066,GO:0035067,GO:0035257,GO:0035258,GO:0035556,GO:0035690,GO:0036211,GO:0040029,GO:0042127,GO:0042221,GO:0042304,GO:0042493,GO:0042770,GO:0042772,GO:0042981,GO:0043009,GO:0043066,GO:0043067,GO:0043069,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043401,GO:0043412,GO:0043627,GO:0043933,GO:0044085,GO:0044087,GO:0044212,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044389,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044430,GO:0044444,GO:0044446,GO:0044450,GO:0044454,GO:0044464,GO:0045717,GO:0045739,GO:0045765,GO:0045766,GO:0045786,GO:0045787,GO:0045814,GO:0045833,GO:0045892,GO:0045893,GO:0045922,GO:0045934,GO:0045935,GO:0045944,GO:0046483,GO:0046599,GO:0046600,GO:0046605,GO:0046606,GO:0046872,GO:0046890,GO:0046914,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048545,GO:0048583,GO:0048584,GO:0048585,GO:0048856,GO:0050681,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051052,GO:0051054,GO:0051055,GO:0051094,GO:0051128,GO:0051129,GO:0051130,GO:0051171,GO:0051172,GO:0051173,GO:0051239,GO:0051240,GO:0051246,GO:0051247,GO:0051248,GO:0051252,GO:0051253,GO:0051254,GO:0051276,GO:0051427,GO:0051493,GO:0051494,GO:0051569,GO:0051570,GO:0051571,GO:0051573,GO:0051716,GO:0051726,GO:0051865,GO:0060255,GO:0060548,GO:0062012,GO:0062014,GO:0065003,GO:0065004,GO:0065007,GO:0070013,GO:0070063,GO:0070316,GO:0070317,GO:0070507,GO:0070510,GO:0070512,GO:0070531,GO:0070646,GO:0070647,GO:0070887,GO:0071103,GO:0071156,GO:0071158,GO:0071310,GO:0071345,GO:0071356,GO:0071383,GO:0071396,GO:0071407,GO:0071417,GO:0071495,GO:0071680,GO:0071681,GO:0071704,GO:0071824,GO:0071840,GO:0071894,GO:0071944,GO:0072331,GO:0072395,GO:0072401,GO:0072422,GO:0072425,GO:0080090,GO:0080134,GO:0080135,GO:0085020,GO:0090068,GO:0090239,GO:0090240,GO:0090304,GO:0097159,GO:0097190,GO:0097193,GO:0097305,GO:0097306,GO:0097327,GO:0098687,GO:0099086,GO:0140096,GO:0140110,GO:1901342,GO:1901360,GO:1901363,GO:1901564,GO:1901576,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1901983,GO:1901984,GO:1901985,GO:1901987,GO:1901988,GO:1902041,GO:1902042,GO:1902115,GO:1902116,GO:1902275,GO:1902494,GO:1902679,GO:1902680,GO:1902749,GO:1902750,GO:1903320,GO:1903322,GO:1903506,GO:1903507,GO:1903508,GO:1904018,GO:1905268,GO:1905269,GO:1990234,GO:1990904,GO:2000026,GO:2000112,GO:2000113,GO:2000377,GO:2000378,GO:2000615,GO:2000617,GO:2000618,GO:2000620,GO:2000756,GO:2000757,GO:2000758,GO:2001020,GO:2001022,GO:2001141,GO:2001233,GO:2001234,GO:2001236,GO:2001237,GO:2001251,GO:2001252
EggNOG free text desc.histone monoubiquitination
EggNOG OGsKOG0978@1,KOG0978@2759
EC2.3.2.27
COG Functional cat.A
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04121
Hectar predicted targeting categoryno signal peptide or anchor
Exons2
Model size177
Cds size174
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig55501.12966.1prot_H-elongata_contig55501.12966.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig55501 897..1674 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930904.5661988-CDS-H-elongata_contig55501:896..9711622930904.5661988-CDS-H-elongata_contig55501:896..971Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig55501 897..971 -
1691679618.806734-CDS-H-elongata_contig55501:896..9711691679618.806734-CDS-H-elongata_contig55501:896..971Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig55501 897..971 -
1622930904.5785375-CDS-H-elongata_contig55501:1575..16741622930904.5785375-CDS-H-elongata_contig55501:1575..1674Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig55501 1576..1674 -
1691679618.8186517-CDS-H-elongata_contig55501:1575..16741691679618.8186517-CDS-H-elongata_contig55501:1575..1674Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig55501 1576..1674 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930904.5920098-UTR-H-elongata_contig55501:1674..16771622930904.5920098-UTR-H-elongata_contig55501:1674..1677Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig55501 1675..1677 -
1691679618.827441-UTR-H-elongata_contig55501:1674..16771691679618.827441-UTR-H-elongata_contig55501:1674..1677Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig55501 1675..1677 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig55501.12966.1

>prot_H-elongata_contig55501.12966.1 ID=prot_H-elongata_contig55501.12966.1|Name=mRNA_H-elongata_contig55501.12966.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=58bp
MIKCSVCQDKKKNRVITKCFHMFCDGCLEQSIKSRNRKCPACTKAYGLDD
VHELWLT*
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mRNA from alignment at H-elongata_contig55501:897..1677-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig55501.12966.1 ID=mRNA_H-elongata_contig55501.12966.1|Name=mRNA_H-elongata_contig55501.12966.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=781bp|location=Sequence derived from alignment at H-elongata_contig55501:897..1677- (Himanthalia elongata Himel1 dioecious)
CAGATGATCAAGTGTTCGGTGTGTCAGGACAAGAAAAAAAATCGAGTCAT
CACGAAGTGTTTCCACATGTTCTGCGACGGCTGTCTCGAGCAGAGCATCA
AGGTGGGCGGCCTTTGTCTTAGATGGTATGACTTTCATTGCAACTATATC
GTGAGGGTGGTTAAACCCTAGGCCTTTAAATAAACCAAGGCACGCATGTT
TCGCCTACCCATCCACCAGACCCTTGCTGGCATCCTCTTTAGATCGCTTT
TAGAACAGTTCTTAATCCTTTTGGAACCGGATTTCAATTTTTATGTTGAA
TTTAGTATGTAAAAAAACAGATCGAGTTCCATAGGGGTTAAACCGGCATA
CCGACATTGCCACTGTATTGATACTGTATTGATATTGCCTTTAAATGATA
TTTTGTATACTGACCCTCATGATGCCAGTCTGTGCCTTCACTAACCCCAA
CCCTCGAAGACAAGAGATACTAGGGACAGTACAATATAATATACTCTATA
ATCTTGCTTTTTTGCCTCCGAAGAAACAGCGTTCAAAGGTAAATCAAGCT
TTATCCTTTTCCCTTACCTCACTAAAGAAGATTTTTATGCCCCACTTCTC
TTTTGTGACTTTTGCTGTTTTGCTAAATGGATATTCGACCTCAACCTCGA
CGTCGACGGGTTCATAATATCGTGAATGGCGTCACCACACGGGCGGGTGG
CGACAGAGCCGTAACCGCAAGTGTCCAGCTTGCACCAAGGCCTATGGATT
GGACGACGTACACGAGCTTTGGCTGACATAA
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Coding sequence (CDS) from alignment at H-elongata_contig55501:897..1677-

>mRNA_H-elongata_contig55501.12966.1 ID=mRNA_H-elongata_contig55501.12966.1|Name=mRNA_H-elongata_contig55501.12966.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=348bp|location=Sequence derived from alignment at H-elongata_contig55501:897..1677- (Himanthalia elongata Himel1 dioecious)
ATGATCAAGTGTTCGGTGTGTCAGGACAAGAAAAAAAATCGAGTCATCAC
GAAGTGTTTCCACATGTTCTGCGACGGCTGTCTCGAGCAGAGCATCAAGA
TGATCAAGTGTTCGGTGTGTCAGGACAAGAAAAAAAATCGAGTCATCACG
AAGTGTTTCCACATGTTCTGCGACGGCTGTCTCGAGCAGAGCATCAAGAG
CCGTAACCGCAAGTGTCCAGCTTGCACCAAGGCCTATGGATTGGACGACG
TACACGAGCTTTGGCTGACATAAAGCCGTAACCGCAAGTGTCCAGCTTGC
ACCAAGGCCTATGGATTGGACGACGTACACGAGCTTTGGCTGACATAA
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