mRNA_H-elongata_contig50822.12337.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig50822.12337.1
Unique NamemRNA_H-elongata_contig50822.12337.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: D8LG49_ECTSI (Succinate dehydrogenase assembly factor 3 n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LG49_ECTSI)

HSP 1 Score: 133 bits (335), Expect = 4.570e-38
Identity = 67/94 (71.28%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:   88 VLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQSGGVGRSMSDEEAANLNDEQREQLFRLKQQTSAS 369
            V RLYKDIL QHR++LP ++RELGDRYVRSEFKAHK ATG+QV QFMH W+SYL+QLR+Q G VGRS+S  + ++LNDEQR+QL RLKQQ S+S
Sbjct:   11 VKRLYKDILRQHRFALPPKHRELGDRYVRSEFKAHKEATGDQVAQFMHAWRSYLEQLRNQGGQVGRSLSAADVSHLNDEQRKQLVRLKQQASSS 104
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: L1JE82_GUITC (Succinate dehydrogenase assembly factor 3 n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JE82_GUITC)

HSP 1 Score: 77.8 bits (190), Expect = 3.520e-16
Identity = 45/95 (47.37%), Postives = 61/95 (64.21%), Query Frame = 1 
Query:   79 RLAVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQ--SGGVGRSMSDEEAANLNDEQREQLFRLKQQ 357
            R A++ LY+ IL  H+  LP   +ELGD YVRSEFK HK A   +V  F   W+ YL  +  Q  SG  GR++ +EEAA+LN EQ+E L RL+++
Sbjct:    7 RTAMISLYRKILRLHKRVLPNEMKELGDSYVRSEFKQHKKAKVGEVKVFARQWKEYLYTIEAQAASGTFGRNLREEEAAHLNQEQKENLKRLEEE 101
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0A7S0W326_9CRYP (Succinate dehydrogenase assembly factor 3 n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0W326_9CRYP)

HSP 1 Score: 77.0 bits (188), Expect = 1.430e-15
Identity = 48/120 (40.00%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:    7 LAFRVLRPRTLSLGKLEDMNSNNGRLAVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQL--RDQSGGVGRSMSDEEAANLNDEQREQLFRLKQQT 360
            L  R  R     LG    M S   + ++L LY+ +L  H+  LPQ  +ELGD+Y RSEFKAHK A   QV QF+  W+ Y+  L  + Q G  GR +   +   ++DEQREQL +L++++
Sbjct:   10 LLLRASRGEGGELGSC-GMASAFSKTSMLNLYRSVLRIHKRVLPQEMKELGDKYARSEFKAHKTAKIGQVKQFVQQWKEYVYTLDIQAQKGAFGRDLDSSKMDKMSDEQREQLKQLEEES 128
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0A7S2G7W5_9STRA (Succinate dehydrogenase assembly factor 3 n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2G7W5_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 6.530e-13
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = 1 
Query:   88 VLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLR--DQSGGVGRSMSDEEAANLNDEQREQLFRLKQQT 360
             L LY+ IL  HR +LP   RELGD+YVRSEF+ H+ A+ E ++ F   W+ YL+ L+  D   G GR + +E +A + DEQ++ L +L ++T
Sbjct:    4 ALSLYRSILRGHR-TLPAEMRELGDKYVRSEFRQHQAASPEFLETFFSEWEGYLETLQTSDSKTGFGRPLGEEISA-MTDEQKQMLLKLAEET 94
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0A7S4MXT2_9EUKA (Succinate dehydrogenase assembly factor 3 n=1 Tax=Prymnesium polylepis TaxID=72548 RepID=A0A7S4MXT2_9EUKA)

HSP 1 Score: 69.3 bits (168), Expect = 6.870e-13
Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 1 
Query:   85 AVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQL-RDQSGGVGRSMSDEEAANLNDEQREQLFRL 348
            + ++LY+ +L  HR  LP  +RELGD YV++EF+ H+ A    + QF   W+ YL Q+ R     VGR MS EE   L+DEQ+ QL ++
Sbjct:   13 SFVKLYRAVLRAHRTHLPAAHRELGDTYVKAEFRLHQDAKPAFLQQFERQWRDYLKQITRPNEEHVGRGMSAEEVEALSDEQKVQLLKI 101
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0A8J2LFQ2_9HEXA (Hypothetical protein n=1 Tax=Allacma fusca TaxID=39272 RepID=A0A8J2LFQ2_9HEXA)

HSP 1 Score: 65.9 bits (159), Expect = 1.960e-11
Identity = 42/100 (42.00%), Postives = 56/100 (56.00%), Query Frame = 1 
Query:   79 RLAVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQSG--------GVGRSMSDEEAANLNDEQREQLFRLKQ 354
            R  VL+LYK IL  HR +LP   +ELG++YVR EF+ HKG   E V  FM  W  Y   L +Q G         +GRS+ +E+  +   +Q  QL+ LKQ
Sbjct:    3 RQRVLQLYKTILRLHR-ALPTELQELGNQYVRDEFRRHKGIAPEHVAPFMMEWAQYCSSLAEQVGLKGPKSAQRLGRSLGEEKLDHFEPQQIYQLYELKQ 101
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: UPI001E278D08 (succinate dehydrogenase assembly factor 3, mitochondrial n=1 Tax=Harmonia axyridis TaxID=115357 RepID=UPI001E278D08)

HSP 1 Score: 61.6 bits (148), Expect = 8.080e-10
Identity = 44/105 (41.90%), Postives = 57/105 (54.29%), Query Frame = 1 
Query:   61 MNSNNGRLAVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQSG---------GVGRSMSDEEAANLNDEQREQLFRL 348
            MN N+ R   L LYK IL  +R  LP   +ELG+ YVR EFK HK    +Q   FM  W +Y   L +Q G         GVG+S+++EE    ND+Q  QL+ L
Sbjct:    1 MNPNHLRQVRL-LYKVILKLNR-GLPSALKELGNTYVRDEFKRHKNCDQDQAKIFMTEWTNYALDLSNQLGVKGIKEGLSGVGKSLTEEELEKFNDDQILQLYDL 103
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: UPI0005CF75C1 (succinate dehydrogenase assembly factor 3, mitochondrial n=1 Tax=Ceratosolen solmsi marchali TaxID=326594 RepID=UPI0005CF75C1)

HSP 1 Score: 61.2 bits (147), Expect = 1.220e-9
Identity = 41/95 (43.16%), Postives = 52/95 (54.74%), Query Frame = 1 
Query:   88 VLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQ--------SGGVGRSMSDEEAANLNDEQREQLFRL 348
            V RLYK IL  HR SLP+  +ELGD YV+SEFK HK     + D F+  W  Y   L DQ        +  +G+S+  E+   L DEQ  QL+ L
Sbjct:   11 VSRLYKMILRLHR-SLPKDIKELGDVYVKSEFKRHKKCNSYEADVFIKEWMDYALSLSDQLNIRNSLNAKSLGKSLKKEDFEILRDEQIYQLYEL 104
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0DBG4_PARTE (Succinate dehydrogenase assembly factor 3 n=1 Tax=Paramecium tetraurelia TaxID=5888 RepID=A0DBG4_PARTE)

HSP 1 Score: 60.8 bits (146), Expect = 1.300e-9
Identity = 36/89 (40.45%), Postives = 57/89 (64.04%), Query Frame = 1 
Query:   91 LRLYKDILSQHRYSLPQRYRELGDRYVRSEFK-AHKGATGEQVDQFMHGWQSYLDQLRDQSGGVGRSMSDEEAANLNDEQREQLFRLKQ 354
            ++++  IL QHR  L    R LGD YV+ EF+ AH+ A  EQ  +F+  W  Y+++L D+S  +GR ++ EE A LN++Q + L +LK+
Sbjct:    9 MQVFYTILKQHRRKLRPEMRILGDAYVKEEFRQAHQKANQEQYIEFLKRWAIYIEEL-DKSKQIGRDLTSEEKALLNEDQIDNLAKLKE 96
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BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Match: A0A813R1I0_9BILA (Succinate dehydrogenase assembly factor 3 n=1 Tax=Brachionus calyciflorus TaxID=104777 RepID=A0A813R1I0_9BILA)

HSP 1 Score: 60.8 bits (146), Expect = 1.720e-9
Identity = 38/104 (36.54%), Postives = 56/104 (53.85%), Query Frame = 1 
Query:   67 SNNGRLAVLRLYKDILSQHRYSLPQRYRELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQSGG-----VGRSMSDEEAANLNDEQREQLFRLKQQTS 363
            S   +  V RLYK I   H+ SLP   +++GD YVR EFK HK A  EQ   FM+ W +Y+  L  Q        +G+ + + +  N N+ Q  QL+ L ++T+
Sbjct:   10 SKQHQQTVKRLYKIIFRLHK-SLPGELKQIGDSYVRHEFKLHKNANPEQTSLFMNEWANYVQILMKQVNPKFKQKIGQDLGESKLNNFNENQVRQLYELFKETT 112
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig50822.12337.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LG49_ECTSI4.570e-3871.28Succinate dehydrogenase assembly factor 3 n=2 Tax=... [more]
L1JE82_GUITC3.520e-1647.37Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]
A0A7S0W326_9CRYP1.430e-1540.00Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]
A0A7S2G7W5_9STRA6.530e-1343.01Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]
A0A7S4MXT2_9EUKA6.870e-1342.70Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]
A0A8J2LFQ2_9HEXA1.960e-1142.00Hypothetical protein n=1 Tax=Allacma fusca TaxID=3... [more]
UPI001E278D088.080e-1041.90succinate dehydrogenase assembly factor 3, mitocho... [more]
UPI0005CF75C11.220e-943.16succinate dehydrogenase assembly factor 3, mitocho... [more]
A0DBG4_PARTE1.300e-940.45Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]
A0A813R1I0_9BILA1.720e-936.54Succinate dehydrogenase assembly factor 3 n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig50822contigH-elongata_contig50822:291..2801 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score134.8
Seed ortholog evalue2.7e-29
Seed eggNOG ortholog2880.D8LG49
Preferred nameACN9
KEGG koko:K08827,ko:K19950
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005758,GO:0006082,GO:0006105,GO:0006109,GO:0006111,GO:0006950,GO:0006979,GO:0006996,GO:0007005,GO:0008150,GO:0008152,GO:0009628,GO:0009889,GO:0009987,GO:0010675,GO:0010906,GO:0015976,GO:0016043,GO:0017144,GO:0019222,GO:0019752,GO:0022607,GO:0031323,GO:0031967,GO:0031970,GO:0031974,GO:0031975,GO:0032501,GO:0033108,GO:0034552,GO:0034553,GO:0034622,GO:0036296,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043255,GO:0043436,GO:0043648,GO:0043933,GO:0044085,GO:0044237,GO:0044281,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050794,GO:0050879,GO:0050881,GO:0050896,GO:0055093,GO:0062012,GO:0065003,GO:0065007,GO:0070013,GO:0070482,GO:0071704,GO:0071840,GO:0080090
EggNOG free text desc.mitochondrial respiratory chain complex II assembly
EggNOG OGsKOG4100@1,KOG4100@2759
EC2.7.11.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001,ko03041,ko04131
Hectar predicted targeting categorymitochondrion
Ec32 ortholog descriptionACN9
Ec32 orthologEc-11_001950.1
Exons3
Model size371
Cds size369
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig50822.12337.1prot_H-elongata_contig50822.12337.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig50822 291..2801 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930834.195722-CDS-H-elongata_contig50822:290..3831622930834.195722-CDS-H-elongata_contig50822:290..383Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 291..383 -
1691679590.4203618-CDS-H-elongata_contig50822:290..3831691679590.4203618-CDS-H-elongata_contig50822:290..383Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 291..383 -
1622930834.2094114-CDS-H-elongata_contig50822:1881..19841622930834.2094114-CDS-H-elongata_contig50822:1881..1984Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 1882..1984 -
1691679590.4309995-CDS-H-elongata_contig50822:1881..19841691679590.4309995-CDS-H-elongata_contig50822:1881..1984Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 1882..1984 -
1622930834.2264395-CDS-H-elongata_contig50822:2628..28011622930834.2264395-CDS-H-elongata_contig50822:2628..2801Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 2629..2801 -
1691679590.4394398-CDS-H-elongata_contig50822:2628..28011691679590.4394398-CDS-H-elongata_contig50822:2628..2801Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig50822 2629..2801 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig50822.12337.1

>prot_H-elongata_contig50822.12337.1 ID=prot_H-elongata_contig50822.12337.1|Name=mRNA_H-elongata_contig50822.12337.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=123bp
CCLAFRVLRPRTLSLGKLEDMNSNNGRLAVLRLYKDILSQHRYSLPQRYR
ELGDRYVRSEFKAHKGATGEQVDQFMHGWQSYLDQLRDQSGGVGRSMSDE
EAANLNDEQREQLFRLKQQTSAS
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mRNA from alignment at H-elongata_contig50822:291..2801-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig50822.12337.1 ID=mRNA_H-elongata_contig50822.12337.1|Name=mRNA_H-elongata_contig50822.12337.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2511bp|location=Sequence derived from alignment at H-elongata_contig50822:291..2801- (Himanthalia elongata Himel1 dioecious)
TGCTGCCTTGCTTTTCGAGTTTTGCGTCCTAGAACCTTGTCTCTGGGGAA
ACTGGAAGATATGAACAGCAACAACGGACGGCTAGCTGTTTTACGGCTCT
ACAAGGATATTCTTAGCCAGCACCGTTATTCTCTCCCGCAAAGATACCGA
GAGCTCGGAGACCGATACGTGAGGTGGGTGCTCCCTCGACGTTGTTACGC
GTTTGTGGCCTCCTAGGGGTAGGCAGGAAGGAGGTCGTGGCGACGAAGCA
AGGACACAAGTCCACATGAGGTGGTTTAGTTCCTGGTTGGCGTGGGCGAT
ACAGCTTGACTTCGAGCTACTATGGCTAGTACTAGTAGTACACTGCTGTG
ACATAAACTGCTGCATATAGAAAGCGAAATACTATGAGTGCTCGACTAAA
TCAAACTTCGAACACTTTACACTGAGACAATCGTCAGCATCAATCCTTCC
AACAAGATTGTGTTCTAGATCTAAATATTAATAGTTACAGAATCCAATCA
CACCCTGTTTGCCTTCAAGGGGAGCCGATTGCGCCCCAAGACTCTTCCCT
CCTTCTTCTTGGACGGGGTTTGATACTCGTTGGGTACAAAATACGGAAAT
AATTTTGAGGTCGGAAAGTGAACCCCGTGTTTTGAATAAAACTGTAGTTA
TCGCGAGCCTCGTGGCAACCTAATATTTCATACTCGTTCGAATAACGTCA
AGATCACAGTAGTTTATGGTTTGAAAGTTGAGTAGGGGGGCTGGGCGGGG
GGAGGGGGGGGGGTGCGAGAAAGATGGTTATCGTCATCTTAGCTTCCCCG
TTGCCCAAATAACACAGATCGGAGTTCAAGGCTCACAAGGGGGCGACGGG
GGAACAGGTGGACCAATTCATGCACGGGTGGCAGAGCTACTTGGACCAGC
TACGAGATCAGAGCGGAGGGGTGCGTGCGCCACCGGGAGAGAGAATGGAA
ATCGAGCCGTCGGTAACAAGAGGGTCCCCCAAGCCACCCGATATTATCCT
GATTGTGCTGCAATCAGAGCAACAAACGCTCGCTTTTCTGTAGGAGTGGC
TCTACTGCTGCTGTAGATTAATCATGTAATCATAGCTCGCTCGTACAGAT
TATATATTTTTCTACTGCATATGTTTTTATTTTTATCTTATCATAAAAGT
CCGAAGACGCGCCTTGAATCCCGCCCGAACAACTAACGAGGTGAAAATTG
GGTTCGATCAAGTGCCGAACGCGAGAACTTGTTTCGCCGCCGTGCTCGGA
TGGATCAGATGCCGCTCTCGAATGCCGCTCTTCCTGTTAACCGCGATTTG
ACAGCCTTTCCACGAAGACGAGGAGAGAGTGTGCCCATCGCCAATCACCG
AAACGAATCGCCTCCGATACTGTCTGTGACTGTATCGCACATGCCCACTC
TACCCATACGAGTAGTGGGTGGGGGAAAAGAGGAACGTACAATAATTTGT
CCATGGGGTTGCCTGAATAGACTGTACCCATTACTCAAGCTACCTTGAGG
ACTATTGGCTCTGTGCCGGCGGACTCTTGGTAGTTAACGCTATCGGTACG
CAATTGCGTGACCTGATGAAATCGAGATTGGCCCGATGGCGGCTGACGGT
AGAAATAGACGTCGTCGCGGAAAACGAGAAGATCCCGAGAGTACGCACCA
TATTCAGCCTGGGTTGAGAGAATGAGCAGGTTGACGCGGGACGAAACTAC
TGAACCCGTCTCACGAGACCAAATTCTCAGGCGCGAACGGGGACACGGGA
AAAATATTTTCCCTTTTTAGCTGAGCAGGAGGCAACCAAATCCGGTTGAT
CTTACTCTGCTATATGTGATGATCAGTGATGGCCATACATACATAATGAT
GTATCGCGCGACAGTCAGTCAGCGTGAACAAAAATAAATATATTTAGGGA
AAAAATATCCTTCCTGTCGTTGTAGTTTTTAGTTTTCCATCGAAGATACA
GCTATTTTCACTCCTTACGGTTGTCGATGCGGTCGATGATGCGCGTTGCC
TGAACAGCGATTTCCGCACTCGAGCGAGAGAGCCGAAGGACCGGAGATGC
GGAATAATGCTCCCGGACAGACATGATTGATCTCCCTTCGTCTGTTCGAT
GAATATATTGCCGCTCCTGTAGCAGAGTCCGCGAGCGGCTGACTCTGTCG
CAGGCGTTATTATACTTTTATCTCACATCGTCGTTATATTGTATATAAAC
TACCAAAGTAATTATCATACCATTGTTTTTGTTGTTGTTGGTCTCACTTT
TAGCAGAAGAATCAACCGGATTTGGTTGCCTTCTGCTCGAGGTCACCTGA
ATAGGGAATATAATTTTCCCCTCTCCCCGTTCGCGCCTGAGGCAATGCGG
TACCGAGGAGGTACTCGAAGCTAGGTCCACGTTTCGCTTCATCAAATATC
CCACGGTCGTGGCCGCAGGTGGGACGGTCGATGAGCGACGAAGAAGCTGC
TAACCTGAACGACGAACAGCGAGAGCAGCTTTTTCGCCTGAAGCAGCAGA
CGTCCGCTTCG
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Coding sequence (CDS) from alignment at H-elongata_contig50822:291..2801-

>mRNA_H-elongata_contig50822.12337.1 ID=mRNA_H-elongata_contig50822.12337.1|Name=mRNA_H-elongata_contig50822.12337.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=738bp|location=Sequence derived from alignment at H-elongata_contig50822:291..2801- (Himanthalia elongata Himel1 dioecious)
TGCTGCCTTGCTTTTCGAGTTTTGCGTCCTAGAACCTTGTCTCTGGGGAA
ACTGGAAGATATGAACAGCAACAACGGACGGCTAGCTGTTTTACGGCTCT
ACAAGGATATTCTTAGCCAGCACCGTTATTCTCTCCCGCAAAGATACCGA
GAGCTCGGAGACCGATACGTGAGTGCTGCCTTGCTTTTCGAGTTTTGCGT
CCTAGAACCTTGTCTCTGGGGAAACTGGAAGATATGAACAGCAACAACGG
ACGGCTAGCTGTTTTACGGCTCTACAAGGATATTCTTAGCCAGCACCGTT
ATTCTCTCCCGCAAAGATACCGAGAGCTCGGAGACCGATACGTGAGATCG
GAGTTCAAGGCTCACAAGGGGGCGACGGGGGAACAGGTGGACCAATTCAT
GCACGGGTGGCAGAGCTACTTGGACCAGCTACGAGATCAGAGCGGAGGGA
TCGGAGTTCAAGGCTCACAAGGGGGCGACGGGGGAACAGGTGGACCAATT
CATGCACGGGTGGCAGAGCTACTTGGACCAGCTACGAGATCAGAGCGGAG
GGGTGGGACGGTCGATGAGCGACGAAGAAGCTGCTAACCTGAACGACGAA
CAGCGAGAGCAGCTTTTTCGCCTGAAGCAGCAGACGTCCGCTTCGGTGGG
ACGGTCGATGAGCGACGAAGAAGCTGCTAACCTGAACGACGAACAGCGAG
AGCAGCTTTTTCGCCTGAAGCAGCAGACGTCCGCTTCG
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