mRNA_H-elongata_contig49704.12179.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig49704.12179.1
Unique NamemRNA_H-elongata_contig49704.12179.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: D7G487_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G487_ECTSI)

HSP 1 Score: 90.1 bits (222), Expect = 1.660e-22
Identity = 42/64 (65.62%), Postives = 56/64 (87.50%), Query Frame = 1 
Query:    1 MSSRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILRVRNIT 192
            MSSRKTRLAS+K+ +N+TRRG+VP+GK++ K+TGY+VGP LL FFLFVV+GSS+FQ+L+    T
Sbjct:    1 MSSRKTRLASEKHLQNVTRRGSVPKGKIENKKTGYAVGPCLLFFFLFVVLGSSLFQVLKAAGAT 64
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A3M6VCY0_9STRA (Uncharacterized protein n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VCY0_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 2.670e-12
Identity = 34/59 (57.63%), Postives = 44/59 (74.58%), Query Frame = 1 
Query:   13 KTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILRVRNI 189
            K RL S+K+  NIT+RG V + + ++K  GYSVGP LL FF+FV+VGSSI QILR  N+
Sbjct:    6 KIRLRSEKHLANITKRGLVSQPEKEEK--GYSVGPLLLGFFVFVLVGSSIIQILRTANL 62
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A4D9DDX7_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DDX7_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 2.940e-12
Identity = 32/57 (56.14%), Postives = 41/57 (71.93%), Query Frame = 1 
Query:    7 SRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILR 177
            SR TRL S+K+ KN+T+RG VP GK  ++     V P LL FF+FVVVGS+ FQI+R
Sbjct:    4 SRATRLRSEKFDKNVTKRGNVPVGKAAKREEAPPVSPVLLGFFVFVVVGSAFFQIIR 60
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A835YYR5_9STRA (Ribosome associated membrane protein RAMP4-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YYR5_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 5.770e-12
Identity = 32/60 (53.33%), Postives = 44/60 (73.33%), Query Frame = 1 
Query:    7 SRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILRVRN 186
            SRKTRLAS+KYA N+T+RG V  G+   K  G  + PY+LAF +FV+VGS++FQI+ +  
Sbjct:    4 SRKTRLASEKYAANVTKRGVVS-GRSKDKDGGSRLNPYVLAFIIFVMVGSTVFQIINMMQ 62
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A8J1ZMH4_9DINO (Hypothetical protein n=1 Tax=Amoebophrya sp. A120 TaxID=2202890 RepID=A0A8J1ZMH4_9DINO)

HSP 1 Score: 63.5 bits (153), Expect = 6.080e-12
Identity = 30/56 (53.57%), Postives = 43/56 (76.79%), Query Frame = 1 
Query:    7 SRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQIL 174
            +RKT+L SD++A+NI  RG V     ++ ++G+ VGP +LAFFLFVVVGS+I QI+
Sbjct:    4 TRKTKLKSDQFARNIRHRGNVATSDREKSKSGFRVGPLVLAFFLFVVVGSAILQII 59
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A1Y1IIN1_KLENI (Ribosome associated membrane protein RAMP4 n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IIN1_KLENI)

HSP 1 Score: 63.2 bits (152), Expect = 8.420e-12
Identity = 33/59 (55.93%), Postives = 43/59 (72.88%), Query Frame = 1 
Query:    1 MSSRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILR 177
            +S+R T++ SDK+ KNI +RG VP  K  +K   Y VGP +L FFLFVVVGS+I QI+R
Sbjct:    3 VSTRHTKVKSDKFQKNILKRGEVP--KTSKKEAKYPVGPIVLGFFLFVVVGSAILQIIR 59
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: A0A2D4BJU9_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BJU9_PYTIN)

HSP 1 Score: 62.0 bits (149), Expect = 1.890e-11
Identity = 31/57 (54.39%), Postives = 42/57 (73.68%), Query Frame = 1 
Query:   19 RLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILRVRNI 189
            RL S+K+  NIT+RG V   + D+K +GY+VGP LL FF+FV+VGSS+ QILR   +
Sbjct:    2 RLRSEKHLANITKRGLV--SQPDKKESGYTVGPVLLGFFIFVLVGSSVIQILRTAQL 56
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: D0MTV7_PHYIT (Uncharacterized protein n=15 Tax=Phytophthora TaxID=4783 RepID=D0MTV7_PHYIT)

HSP 1 Score: 61.2 bits (147), Expect = 4.410e-11
Identity = 32/59 (54.24%), Postives = 42/59 (71.19%), Query Frame = 1 
Query:   13 KTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILRVRNI 189
            K RL S+K+  NIT+RG V + + + K  GYSVGP L+ FFLFV+VGSS+ QILR   +
Sbjct:    6 KMRLRSEKHLANITKRGQVSQPQKEDK--GYSVGPILMGFFLFVLVGSSVIQILRTAQL 62
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BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: W7U255_9STRA (Endoplasmic reticulum, stress-associated Ramp4 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7U255_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 1.120e-10
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 1 
Query:    7 SRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILR 177
            SR  RL S+K+ KN+T+RG VP GK  ++     V P LL FF+FVVVGS+ FQI+R
Sbjct:   69 SRAIRLRSEKFDKNVTKRGNVPVGKAAKREEAPPVSPVLLGFFVFVVVGSAFFQIIR 125
[Close]
BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Match: K3WKY6_GLOUD (Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4782 RepID=K3WKY6_GLOUD)

HSP 1 Score: 60.1 bits (144), Expect = 1.260e-10
Identity = 31/55 (56.36%), Postives = 40/55 (72.73%), Query Frame = 1 
Query:   13 KTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVVGSSIFQILR 177
            K RL S+K+  NIT+RG V   K ++  +GY VGP LL FF+FV+VGSS+ QILR
Sbjct:    6 KMRLRSEKHLANITKRGMV--SKPEKNESGYGVGPVLLGFFVFVLVGSSVIQILR 58
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig49704.12179.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G487_ECTSI1.660e-2265.63Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A3M6VCY0_9STRA2.670e-1257.63Uncharacterized protein n=1 Tax=Peronospora effusa... [more]
A0A4D9DDX7_9STRA2.940e-1256.14Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A835YYR5_9STRA5.770e-1253.33Ribosome associated membrane protein RAMP4-domain-... [more]
A0A8J1ZMH4_9DINO6.080e-1253.57Hypothetical protein n=1 Tax=Amoebophrya sp. A120 ... [more]
A0A1Y1IIN1_KLENI8.420e-1255.93Ribosome associated membrane protein RAMP4 n=1 Tax... [more]
A0A2D4BJU9_PYTIN1.890e-1154.39Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
D0MTV7_PHYIT4.410e-1154.24Uncharacterized protein n=15 Tax=Phytophthora TaxI... [more]
W7U255_9STRA1.120e-1054.39Endoplasmic reticulum, stress-associated Ramp4 n=1... [more]
K3WKY6_GLOUD1.260e-1056.36Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig49704contigH-elongata_contig49704:101..3854 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score61.2
Seed ortholog evalue2e-07
Seed eggNOG ortholog325452.fgenesh_scip_prom.46568.1077
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0006464,GO:0006486,GO:0006807,GO:0006950,GO:0006986,GO:0007154,GO:0007165,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009987,GO:0010033,GO:0012505,GO:0019538,GO:0023052,GO:0030968,GO:0033554,GO:0034620,GO:0034645,GO:0034976,GO:0035966,GO:0035967,GO:0036211,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043413,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0065007,GO:0070085,GO:0070887,GO:0071310,GO:0071704,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Ribosome associated membrane protein RAMP4
EggNOG OGs2CZD0@1,2S9SE@2759,3QGRA@4776
COG Functional cat.U
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categorysignal anchor
Ec32 ortholog descriptionStress-associated endoplasmic reticulum protein
Ec32 orthologEc-02_001730.1
Exons2
Model size192
Cds size192
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930818.4259074-CDS-H-elongata_contig49704:100..2261622930818.4259074-CDS-H-elongata_contig49704:100..226Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49704 101..226 -
1691679583.373206-CDS-H-elongata_contig49704:100..2261691679583.373206-CDS-H-elongata_contig49704:100..226Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49704 101..226 -
1622930818.4400196-CDS-H-elongata_contig49704:3788..38541622930818.4400196-CDS-H-elongata_contig49704:3788..3854Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49704 3789..3854 -
1691679583.3907275-CDS-H-elongata_contig49704:3788..38541691679583.3907275-CDS-H-elongata_contig49704:3788..3854Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig49704 3789..3854 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig49704.12179.1prot_H-elongata_contig49704.12179.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig49704 101..3854 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig49704.12179.1

>prot_H-elongata_contig49704.12179.1 ID=prot_H-elongata_contig49704.12179.1|Name=mRNA_H-elongata_contig49704.12179.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=64bp
MSSRKTRLASDKYAKNITRRGTVPRGKMDQKRTGYSVGPYLLAFFLFVVV
GSSIFQILRVRNIT
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mRNA from alignment at H-elongata_contig49704:101..3854-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig49704.12179.1 ID=mRNA_H-elongata_contig49704.12179.1|Name=mRNA_H-elongata_contig49704.12179.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=3754bp|location=Sequence derived from alignment at H-elongata_contig49704:101..3854- (Himanthalia elongata Himel1 dioecious)
ATGTCCTCCCGCAAGACGCGCCTGGCGAGCGATAAATACGCCAAGAACAT
CACACGACGTGGCACGGTAAGATATTTTTGTGCGGCGAGAGGAACCGAAC
CCGATGAACAGCAGCACCTCTTAGGCGATGTGTGTTCGGCTGTCCTTGAG
CATTAGACCCTTGGGGTCGCGAGGCCTTTGACTCGTGTGTTATGGTTATT
GGTCGCCCAGTTCGTCTTCCACAATTTAGATAGTTCTTTCCCATCGATGG
TGTGGCTCGAAGCAAGCGCTATCTTGCTCCCTTCGACCGCTCTTTTCTCT
TTTCCTGATCATAAAGCAGCCAAAGTCAATCTAAATTATAGGGGCATTAA
AGAACTGCCGATATCGGGGTGATTTGGTGCCTGTAATTCCACGAACACCT
TCACTTTAGCCTGAGAATGTGTTCTTGTAATTAGAAGCATCAAATCACCC
CGATATCAGCACTCTTTAAATGATGTGTCCAGCGGTAGTGCAAGAGTCAT
CCGCCACACACCCCCATCGCCCTCCCCCTTTCCCTATCACATGGACAAAT
GCTCCGTTATACTGTCGAAAACACCAACACCAGTACTACTCGTAGTACCG
AAGGTCTCACCTGAACAAAGGCTTTATTTTAGTATCAAGAAATCGGAAAT
TGATTATTGTGACTACGGGGACATATAAGGGTTCATTCCCGGGGTTTCCA
CAATCAAATTAAGGTTCTCAGAGCCCAAGGGTGGTTGTGTGTGGGTTAGG
GGGCGGATTGGGTCAGCCAGTGTATATAGGTTTTTAAGGAACGGCTATTT
TACTCTGCATGAAATTAACGTCGTTTAACGTTTTTTGCATTTTTTTCCTG
TAGTCACAAACTAAATTGTGACCACGTAGTCACAGTTATCACTCCACGAA
GACATCGAGGTGGAGGCGTCTGAAACACATCTAGCATTAGACTATAGTTG
TATGTATGTATGTATGTATGTATAATCGCACTTTTAGCAGAAGGATCAAC
CTGATTTCGTCTCCTTCTGCTCGTGGTCAGCTGAACAGGTCCCCATAAGG
GGGAGTAATTGCAAACCGGCGTGGCGCCCCCTTTACGTGTCTTGCCGGTT
TGCGCGGTTTAATGCAAATCGATAAAAAGGGAAAATGAATTTCCCCCTGT
CCCCGTTCGCGCCTGAGAATTTGATCTGGTGAGACGGGTTCAGCAGTCCC
GTCCCGCGTCAGCCGGCTCAGTATCTCAACCCAGGCTGAATCTGGTGAGT
GCTCCTGGGATCCTCTCGATCCTTCCGTTTTCCGCAACGGCCTCCATCTC
TACCGTCAACCGCGATTGGGTCAATCTCGATTTTATCAGGTCACGCAATT
ACGTACCGATAGAAAGTCCGCCGGCACTGAGTTAATAGTCCTCAAGGTAG
CTTGAGTAACGGGTGCAGCCTTTTCAGGTAATTAACTCCATGGACCAAGT
AATGTACGCCTCTATTTTCCCACACCCACTATGCCCACTATGGGAAGAGT
GGGATATTACATAAAACAGGTGAGTGGTTATTCAAACTGCTATTAATAAT
TAACAAGAGGGCTTGAAGAAGTTCATACCCTGGTATATTTAGTTATTTTT
GAAAACTTGTCAGCGTCCTACCGCATCTCGTGATCTCATATTGCCCGAGC
AATCTAAATCCCTTACTTAATCTACAATAATCGTAACTGCGTTAAGCCTA
ATACTATTTTAAGTAATTATTAGTATTATTATTAACATCCATAGCATTAA
TAGTACTACGGGAGTCATAAGTTCTAATGACACCAGGGTTGAATCCATTG
TTTCTGTAAAAAAAAATGGGGAACAGGATTAAGCAAGCAAAACAAAACAA
AGCTATTGATAGCCGACATTGATTGGTTCGCGAGGTGGTGTTGGAGAAGA
CCTCTTAAAGTTGCAGACTTGAAGAATGTGTAAGCTCTTGGTTATTGGTA
CTAAGAGGGGTTTGAATGAGTTTGCATGTTTTTTAGAAAAGAAAAGATAA
TACGGAGGCGTTAACCGGGGAGATGTGGGTATATTCCCCTGGTACCACGG
GCAGGATTGACAAGATGTGTAGCGTAGCGCACAGGCGGAATCGGTGAGCG
AGCCTGTTGACCAGCTCTGTCGACAAAAAGTGAGGCCAAACAAAAAATGT
ACAGTAGAATGTCAGTATTGGAGGGGGGGGTATCTCCCAGTAGTTGCCCT
GTTAATCGCACAAATAGCGGTCATCACCCGCGAGTACAAGTATTTCCCGT
CCAGCATGTGTTATGTTTTGTCATCCAACATCTATTAATAACGAATAAGA
CAATCACGACAAACAAGCGCAAGGGGTCACTTCTCAGGCGTCAGCTTGAA
ATACAGCAGTGGGCGGTTGGTCACGACTGACGACGAGCCGTTAGAGACTC
GGGAGGATGCGGGATATGTGTACAGTAACGATATCTACCAATCTATATAT
TTACTATCAACAATCACTATAGAGCAAGACTGCCCCACAATGTGTGTGGC
CTTTTGGGGGTGGAAAACCCTCCCCCCTCTCACGCCCGTCACACATCAAG
GTACCTTACACAAAACAAAGTCATCCCTTCGCACGAAGGTACTCTTAAGA
AAGCGCCAGAAGGATGGTCTATCTCAAGGGATGATAAGATAAAACTCCGA
TGTGGCCTGGACGGGTTTCCACAACTCCGAGATTGGCAACGCACATTGCG
CAATCACCCACATACGCACCACTTTAACGGTCTATACCCTTCAAGCAATG
TATAGCACACACACCCTCCCCACGGGGGGGTGGGCCTTGGTGCCGCAGGC
GCGCGAGATTTCGCGGGTAGCGAGCGCCGCCTTTTTAGCAAAGAACTGCG
TCAAGAATATAAATAAACTGGACACGCAGTTAGGGGAAGACAAAAAAAAT
ACTTCTGTCGACAGGGCCAGGCCAGGCCGTTCGTTGTTACCAGTCCTGGC
TGCTGCAACCCTTCGCTTTGCTTCTTATTCCGCCTGTGGTTTCTGGGGGG
TCGATGTTCCCCCCATCTCTCCACGGTCGCCTTAAGATTCACATGTGTCG
CCTCGGTGGTTCAGTTTGTAACCTCGAAACGTTAGGAAGTCGGGACGTGG
TGAGTTCGAATCTCGGCGCAGTCACACGAACCGGGATCTATCTCTCTCAC
ATCATGCCCCAACACGTGTAGGAAAGGAATGGCTAAAAAATCCTTCTCGC
TATACAAAAAAATATATATGGCAAACACATCGATGAAGGAAGGTGGAGAG
ATCATGTGTGACGTTCCTCTCCGTTACCCCGTTACCCCGGCTGATGCGCC
CACAAGACTAACGTTTAACGTCCAGAATAATAATAAATGGGATGACAACA
TTTCGAATTTTTGTATCGCGCGGGGCAAGTTCAGCACTCACTCCTATTGC
ACACCTCGACGATCCTTCGAGAAAGCGGATGATTAGTGGTCGAACGTTTT
GCTTTTTAACCCCCGCGGTATCATCGGGGGAAGAAAAGTCGTACCACTCG
CCATGCTAGGCTCTAATTTTCTATCCGTCGCTGCTGCTGTTGTTGCTGCT
GATGAGTCCTTGTGCGCGCACTCGGCGGCCCTTCCGCTCGACGCCGTGCC
TCCCTGTCTGCGTTCGTCCCTATGGCAGGTTCCTCGGGGCAAGATGGACC
AAAAGAGGACGGGGTACTCGGTGGGGCCTTACCTTCTCGCCTTCTTTTTG
TTCGTCGTGGTGGGGTCCAGCATCTTCCAAATCCTGAGGGTGAGAAATAT
AACA
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Coding sequence (CDS) from alignment at H-elongata_contig49704:101..3854-

>mRNA_H-elongata_contig49704.12179.1 ID=mRNA_H-elongata_contig49704.12179.1|Name=mRNA_H-elongata_contig49704.12179.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=384bp|location=Sequence derived from alignment at H-elongata_contig49704:101..3854- (Himanthalia elongata Himel1 dioecious)
ATGTCCTCCCGCAAGACGCGCCTGGCGAGCGATAAATACGCCAAGAACAT
CACACGACGTGGCACGATGTCCTCCCGCAAGACGCGCCTGGCGAGCGATA
AATACGCCAAGAACATCACACGACGTGGCACGGTTCCTCGGGGCAAGATG
GACCAAAAGAGGACGGGGTACTCGGTGGGGCCTTACCTTCTCGCCTTCTT
TTTGTTCGTCGTGGTGGGGTCCAGCATCTTCCAAATCCTGAGGGTGAGAA
ATATAACAGTTCCTCGGGGCAAGATGGACCAAAAGAGGACGGGGTACTCG
GTGGGGCCTTACCTTCTCGCCTTCTTTTTGTTCGTCGTGGTGGGGTCCAG
CATCTTCCAAATCCTGAGGGTGAGAAATATAACA
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