mRNA_H-elongata_contig201986.5714.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig201986.5714.1
Unique NamemRNA_H-elongata_contig201986.5714.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3GCN4_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=2 Tax=Phytophthora TaxID=4783 RepID=A0A6A3GCN4_9STRA)

HSP 1 Score: 115 bits (289), Expect = 4.250e-28
Identity = 74/172 (43.02%), Postives = 105/172 (61.05%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K++ YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:   69 KFRIKDMGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGQENAKPYLTPLEPGV-QLTKADEPQTEEDKAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVHYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLML 238
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3V7V5_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=5 Tax=Phytophthora TaxID=4783 RepID=A0A6A3V7V5_9STRA)

HSP 1 Score: 115 bits (287), Expect = 8.180e-28
Identity = 73/171 (42.69%), Postives = 105/171 (61.40%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEEQMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KD+G  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +E +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+++D+    D RRSVSG +LML
Sbjct:  132 KFRIKDLGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGRENAKPCLTPLEPGV-NLTKADEPQT-EEAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSNADWAGNRDDRRSVSGMLLML 299
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BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3GI70_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=9 Tax=Phytophthora TaxID=4783 RepID=A0A6A3GI70_9STRA)

HSP 1 Score: 114 bits (286), Expect = 9.120e-28
Identity = 73/172 (42.44%), Postives = 105/172 (61.05%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     + + R + I    Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:   21 KFRIKDMGRARFILGIEIDYDMERRTLGISHKAYTESIIKKFGQENAKPCLTPLEPGV-QLTKADEPQTEEDKAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLML 190
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6G0QQ26_9STRA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 n=20 Tax=Phytophthora TaxID=4783 RepID=A0A6G0QQ26_9STRA)

HSP 1 Score: 117 bits (294), Expect = 1.390e-27
Identity = 74/172 (43.02%), Postives = 107/172 (62.21%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     N + R + I Q  Y ESI++K+  +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ +  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:  602 KFRIKDMGRARFILGIEIDYNMERRTLGISQKAYTESIIKKVGQENAKPCLTPLEPGV-QLTKADEPQTEEDKAKMKSKPYRSLVGSLMYLSCGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLML 771
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A4F359_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A4F359_9STRA)

HSP 1 Score: 114 bits (286), Expect = 3.120e-27
Identity = 73/172 (42.44%), Postives = 106/172 (61.63%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LM+
Sbjct:  101 KFRIKDMGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGQENAKPCLTPLEPGV-QLTKADEPQIEEDKAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLMM 270
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3HKQ8_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Phytophthora rubi TaxID=129364 RepID=A0A6A3HKQ8_9STRA)

HSP 1 Score: 114 bits (285), Expect = 3.190e-27
Identity = 73/172 (42.44%), Postives = 105/172 (61.05%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPK-EEQMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KD+G  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  + + +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:  132 KFRIKDLGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGQENAKPCLTPLEPGV-QLTKADEPQTEEVKAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLML 301
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3VCN8_9STRA (Reverse transcriptase Ty1/copia-type domain-containing protein n=3 Tax=Phytophthora TaxID=4783 RepID=A0A6A3VCN8_9STRA)

HSP 1 Score: 113 bits (283), Expect = 6.110e-27
Identity = 73/172 (42.44%), Postives = 105/172 (61.05%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +      + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:   77 KFRIKDMGRARFILSIEIDYDIERRTLGISQKAYTESIIKKFGQENTKPCLTPLEPGV-QLTKADEPQTEEDKAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNRDDRRSVSGMLLML 246
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3DSS8_9STRA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 n=13 Tax=Phytophthora TaxID=4783 RepID=A0A6A3DSS8_9STRA)

HSP 1 Score: 115 bits (288), Expect = 7.950e-27
Identity = 73/172 (42.44%), Postives = 106/172 (61.63%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ +  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:  358 KFRIKDMGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGQENAKPCLTPLEPGV-QLTKADEPQTEEDKAKMKSKPYRSLVGSLMYLSCGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSDADWAGNWDDRRSVSGMLLML 527
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3Q978_9STRA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 n=1 Tax=Phytophthora fragariae TaxID=53985 RepID=A0A6A3Q978_9STRA)

HSP 1 Score: 115 bits (287), Expect = 1.200e-26
Identity = 73/171 (42.69%), Postives = 105/171 (61.40%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEEQMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KD+G  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +E +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+++D+    D RRSVSG +LML
Sbjct:  526 KFRIKDLGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGRENAKPCLTPLEPGV-NLTKADEPQT-EEAKMKSKPYRSLVGSLMYLACGTRPDISVAVAKLSRFLENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGTQLEAYSNADWAGNRDDRRSVSGMLLML 693
[Close]
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Match: A0A6A3YGI7_9STRA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 n=11 Tax=Phytophthora TaxID=4783 RepID=A0A6A3YGI7_9STRA)

HSP 1 Score: 115 bits (287), Expect = 1.220e-26
Identity = 74/172 (43.02%), Postives = 106/172 (61.63%), Query Frame = 1 
Query:    1 RFKLKDMGDTRYYMGC----NRKAREVKIGQHLYVESIVEKL--DIKKESKIPATSGVPTLSKADEPRNPKEE-QMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENPGPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRRSVSGAVLML 495
            +F++KDMG  R+ +G     + + R + I Q  Y ESI++K   +  K    P   GV  L+KADEP+  +++ +M+  PYR  VG+LM+ A  TR DI+ AV  ++RF ENPG     A +K+++YLL TKD GI Y G   G  +EAY+D+D+    D RRSVSG +LML
Sbjct:  624 KFRIKDMGRARFILGIEIDYDMERRTLGISQKAYTESIIKKFGQENAKPCLTPLEPGV-QLTKADEPQTEEDKAKMKLKPYRSLVGSLMYLARGTRPDISVAVAKLSRFPENPGEKHWDAGIKVVRYLLKTKDVGIVYDGL-LGAQLEAYSDADWAGNRDDRRSVSGMLLML 793
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig201986.5714.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6A3GCN4_9STRA4.250e-2843.02Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6A3V7V5_9STRA8.180e-2842.69Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6A3GI70_9STRA9.120e-2842.44Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6G0QQ26_9STRA1.390e-2743.02Retrovirus-related Pol polyprotein from transposon... [more]
A0A6A4F359_9STRA3.120e-2742.44Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6A3HKQ8_9STRA3.190e-2742.44Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6A3VCN8_9STRA6.110e-2742.44Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A6A3DSS8_9STRA7.950e-2742.44Retrovirus-related Pol polyprotein from transposon... [more]
A0A6A3Q978_9STRA1.200e-2642.69Retrovirus-related Pol polyprotein from transposon... [more]
A0A6A3YGI7_9STRA1.220e-2643.02Retrovirus-related Pol polyprotein from transposon... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig201986contigH-elongata_contig201986:598..1092 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score113.2
Seed ortholog evalue1.1e-22
Seed eggNOG ortholog164328.Phyra85763
EggNOG free text desc.transposition, RNA-mediated
EggNOG OGs3QGIS@4776,COG2801@1,KOG0017@2759
COG Functional cat.L
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons1
Model size495
Cds size477
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930136.7122324-UTR-H-elongata_contig201986:597..6151622930136.7122324-UTR-H-elongata_contig201986:597..615Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig201986 598..615 +
1691679294.9525118-UTR-H-elongata_contig201986:597..6151691679294.9525118-UTR-H-elongata_contig201986:597..615Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig201986 598..615 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930136.7278395-CDS-H-elongata_contig201986:615..10921622930136.7278395-CDS-H-elongata_contig201986:615..1092Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig201986 616..1092 +
1691679294.9653628-CDS-H-elongata_contig201986:615..10921691679294.9653628-CDS-H-elongata_contig201986:615..1092Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig201986 616..1092 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig201986.5714.1prot_H-elongata_contig201986.5714.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig201986 616..1092 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig201986.5714.1

>prot_H-elongata_contig201986.5714.1 ID=prot_H-elongata_contig201986.5714.1|Name=mRNA_H-elongata_contig201986.5714.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=159bp
MGDTRYYMGCNRKAREVKIGQHLYVESIVEKLDIKKESKIPATSGVPTLS
KADEPRNPKEEQMRKFPYREAVGALMWTATMTRSDIASAVRVVARFCENP
GPARKKAVMKIMQYLLHTKDWGITYGGQDCGLCMEAYTDSDFGACLDTRR
SVSGAVLML
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mRNA from alignment at H-elongata_contig201986:598..1092+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig201986.5714.1 ID=mRNA_H-elongata_contig201986.5714.1|Name=mRNA_H-elongata_contig201986.5714.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=495bp|location=Sequence derived from alignment at H-elongata_contig201986:598..1092+ (Himanthalia elongata Himel1 dioecious)
AGGTTCAAATTGAAGGACATGGGTGACACCAGGTACTACATGGGTTGTAA
CCGCAAGGCGCGCGAGGTGAAGATCGGCCAACACCTGTACGTGGAATCGA
TAGTGGAAAAGCTCGACATCAAGAAGGAAAGCAAGATACCGGCTACGTCG
GGGGTGCCAACACTCTCAAAGGCGGACGAGCCGCGGAACCCGAAGGAGGA
GCAAATGAGGAAGTTTCCATACCGGGAAGCAGTGGGGGCGCTTATGTGGA
CGGCGACGATGACACGGTCGGACATTGCGAGCGCAGTACGAGTTGTGGCC
AGGTTCTGCGAAAACCCTGGGCCGGCGCGTAAAAAGGCAGTGATGAAGAT
TATGCAGTATCTCCTTCACACGAAGGATTGGGGGATTACGTACGGTGGAC
AGGACTGTGGACTCTGCATGGAGGCATACACGGACTCGGACTTTGGAGCC
TGCCTGGATACGAGACGATCGGTGTCAGGTGCGGTGTTGATGCTG
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Coding sequence (CDS) from alignment at H-elongata_contig201986:598..1092+

>mRNA_H-elongata_contig201986.5714.1 ID=mRNA_H-elongata_contig201986.5714.1|Name=mRNA_H-elongata_contig201986.5714.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=954bp|location=Sequence derived from alignment at H-elongata_contig201986:598..1092+ (Himanthalia elongata Himel1 dioecious)
ATGGGTGACACCAGGTACTACATGGGTTGTAACCGCAAGGCGCGCGAGGT
GAAGATCGGCCAACACCTGTACGTGGAATCGATAGTGGAAAAGCTCGACA
TCAAGAAGGAAAGCAAGATACCGGCTACGTCGGGGGTGCCAACACTCTCA
AAGGCGGACGAGCCGCGGAACCCGAAGGAGGAGCAAATGAGGAAGTTTCC
ATACCGGGAAGCAGTGGGGGCGCTTATGTGGACGGCGACGATGACACGGT
CGGACATTGCGAGCGCAGTACGAGTTGTGGCCAGGTTCTGCGAAAACCCT
GGGCCGGCGCGTAAAAAGGCAGTGATGAAGATTATGCAGTATCTCCTTCA
CACGAAGGATTGGGGGATTACGTACGGTGGACAGGACTGTGGACTCTGCA
TGGAGGCATACACGGACTCGGACTTTGGAGCCTGCCTGGATACGAGACGA
TCGGTGTCAGGTGCGGTGTTGATGCTGATGGGTGACACCAGGTACTACAT
GGGTTGTAACCGCAAGGCGCGCGAGGTGAAGATCGGCCAACACCTGTACG
TGGAATCGATAGTGGAAAAGCTCGACATCAAGAAGGAAAGCAAGATACCG
GCTACGTCGGGGGTGCCAACACTCTCAAAGGCGGACGAGCCGCGGAACCC
GAAGGAGGAGCAAATGAGGAAGTTTCCATACCGGGAAGCAGTGGGGGCGC
TTATGTGGACGGCGACGATGACACGGTCGGACATTGCGAGCGCAGTACGA
GTTGTGGCCAGGTTCTGCGAAAACCCTGGGCCGGCGCGTAAAAAGGCAGT
GATGAAGATTATGCAGTATCTCCTTCACACGAAGGATTGGGGGATTACGT
ACGGTGGACAGGACTGTGGACTCTGCATGGAGGCATACACGGACTCGGAC
TTTGGAGCCTGCCTGGATACGAGACGATCGGTGTCAGGTGCGGTGTTGAT
GCTG
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