mRNA_H-akashiwo_Contig1024.5.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1024.5.1
Unique NamemRNA_H-akashiwo_Contig1024.5.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A7S3Y0G4_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y0G4_HETAK)

HSP 1 Score: 162 bits (410), Expect = 5.600e-48
Identity = 78/78 (100.00%), Postives = 78/78 (100.00%), Query Frame = 2
Query:    2 PMQLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            PMQLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR
Sbjct:   79 PMQLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 156          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A836CB75_9STRA (ClpP/crotonase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB75_9STRA)

HSP 1 Score: 90.5 bits (223), Expect = 2.630e-20
Identity = 42/78 (53.85%), Postives = 54/78 (69.23%), Query Frame = 2
Query:    2 PMQLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            P QLRC++L  S+DK+FS GRDLK+S  H T E+R  YL     +  A+H L  PTIAC+ G   GWG+E+ALACD+R
Sbjct:   52 PGQLRCVMLRSSSDKAFSAGRDLKLSAKHTTEEQRRDYLLNCVDSANAMHQLRAPTIACMTGAAYGWGVEVALACDIR 129          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: W7U9P9_9STRA (3-hydroxybutyryl-dehydratase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U9P9_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.420e-19
Identity = 43/76 (56.58%), Postives = 56/76 (73.68%), Query Frame = 2
Query:    8 QLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            ++R  ++TG T ++FS GRDLK S LH T E+R +Y+KL   +V A+H L +PTIAC+ G   GWGLELALACDLR
Sbjct:  105 KVRVCVVTGGT-RAFSTGRDLKDSKLHTTLEKRERYMKLALDSVMAVHRLPLPTIACLSGPAFGWGLELALACDLR 179          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A3D6DPW1_9PROT (Enoyl-CoA hydratase n=1 Tax=Candidatus Accumulibacter sp. TaxID=2053492 RepID=A0A3D6DPW1_9PROT)

HSP 1 Score: 72.4 bits (176), Expect = 1.670e-13
Identity = 36/77 (46.75%), Postives = 50/77 (64.94%), Query Frame = 2
Query:    8 QLRCLILTGSTDKSFSVGRDLK-ISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            QLRC+IL G  D++F+ G D++      +T ER + Y    G+A+QA+ D  +PTIA V+G C G GLE+A  CDLR
Sbjct:   47 QLRCVILRGDGDQAFAAGGDIEEFLSRRDTLERAMAYHAQVGEALQAVFDCRLPTIALVQGACIGGGLEIAAQCDLR 123          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: UPI001668640E (enoyl-CoA hydratase-related protein n=1 Tax=Terasakiella brassicae TaxID=1634917 RepID=UPI001668640E)

HSP 1 Score: 66.2 bits (160), Expect = 3.370e-11
Identity = 34/76 (44.74%), Postives = 50/76 (65.79%), Query Frame = 2
Query:   11 LRCLILTGSTDKSFSVGRDLKISHLHETSERRLK-YLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            LRCLIL G+ D++F+ G D+       +S  + K Y ++  + VQAI + + PTIA ++G CTG GLE+A ACD+R
Sbjct:   48 LRCLILRGAGDEAFAAGADISEFPEQRSSAAQAKAYGEVVTRTVQAIEECQHPTIAMIKGACTGGGLEIACACDMR 123          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A355B038_9BACT (Enoyl-CoA hydratase n=1 Tax=Nitrospinae bacterium TaxID=2026769 RepID=A0A355B038_9BACT)

HSP 1 Score: 66.2 bits (160), Expect = 3.400e-11
Identity = 33/73 (45.21%), Postives = 45/73 (61.64%), Query Frame = 2
Query:   17 CLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            C++L     ++FS GRDLK S  H T E   +Y  LG + V    +L +PTIA + G   GWG+E+ALACD+R
Sbjct:   51 CVVLLTGEGRAFSTGRDLKESASH-TKEDADRYQLLGMETVTKWENLPMPTIAAINGHAFGWGMEIALACDIR 122          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A1H4GYU5_9BACI (Enoyl-CoA hydratase/carnithine racemase n=2 Tax=Thalassobacillus TaxID=331971 RepID=A0A1H4GYU5_9BACI)

HSP 1 Score: 65.9 bits (159), Expect = 4.720e-11
Identity = 35/74 (47.30%), Postives = 45/74 (60.81%), Query Frame = 2
Query:   14 RCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            RCLI+TGS  K+F  G DLK      T +  ++ + L G  +  +  +EVPTIA + G   G GLELALACDLR
Sbjct:   50 RCLIITGSGGKAFCAGADLK-ERAGMTEQEVVETVTLIGDTIHRVEQIEVPTIAVLNGVAFGGGLELALACDLR 122          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A661BRA8_9GAMM (Uncharacterized protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A661BRA8_9GAMM)

HSP 1 Score: 64.7 bits (156), Expect = 1.280e-10
Identity = 36/74 (48.65%), Postives = 45/74 (60.81%), Query Frame = 2
Query:   11 LRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAV-QAIHDLEVPTIACVEGQCTGWGLELALACD 229
            +RCLI+TG+ DKSF  G D+K   +   S ++ + L   GQ V  AI  L VP IACV G   G G+EL LACD
Sbjct:   50 IRCLIITGAGDKSFVAGADIK--EMQNMSPQQARTLSNRGQEVFNAIEQLAVPVIACVNGFALGGGMELVLACD 121          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A850KCM8_9HYPH (Enoyl-CoA hydratase/isomerase family protein n=1 Tax=Methylocystaceae bacterium TaxID=2052174 RepID=A0A850KCM8_9HYPH)

HSP 1 Score: 63.5 bits (153), Expect = 3.370e-10
Identity = 33/76 (43.42%), Postives = 48/76 (63.16%), Query Frame = 2
Query:   11 LRCLILTGSTDKSFSVGRDLKISHLHETSERRLK-YLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            LRC+IL G+ D +F+ G D+       +S  + K Y  +  + V+AI + + PTIA ++G CTG GLE+A ACDLR
Sbjct:   48 LRCIILRGAGDDAFAAGADISEFPEERSSAAQAKAYGDIVTRTVKAIEECQHPTIAMIKGACTGGGLEIACACDLR 123          
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Match: A0A241WAA0_9GAMM (Enoyl-CoA hydratase n=1 Tax=Acinetobacter sp. ANC 3813 TaxID=1977873 RepID=A0A241WAA0_9GAMM)

HSP 1 Score: 63.2 bits (152), Expect = 4.560e-10
Identity = 32/75 (42.67%), Postives = 42/75 (56.00%), Query Frame = 2
Query:   11 LRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHDLEVPTIACVEGQCTGWGLELALACDLR 235
            LR  I TG   ++F  G D+     H ++ER L  ++ G    Q IH L  PT+AC++G   G GLELALAC  R
Sbjct:   45 LRAAIFTGDGTQAFCAGADITELQ-HRSAERHLDTIRKGQNLFQQIHQLNFPTLACIQGAALGGGLELALACTFR 118          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1024.5.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3Y0G4_HETAK5.600e-48100.00Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A836CB75_9STRA2.630e-2053.85ClpP/crotonase-like domain-containing protein n=1 ... [more]
W7U9P9_9STRA1.420e-1956.583-hydroxybutyryl-dehydratase n=2 Tax=Monodopsidace... [more]
A0A3D6DPW1_9PROT1.670e-1346.75Enoyl-CoA hydratase n=1 Tax=Candidatus Accumulibac... [more]
UPI001668640E3.370e-1144.74enoyl-CoA hydratase-related protein n=1 Tax=Terasa... [more]
A0A355B038_9BACT3.400e-1145.21Enoyl-CoA hydratase n=1 Tax=Nitrospinae bacterium ... [more]
A0A1H4GYU5_9BACI4.720e-1147.30Enoyl-CoA hydratase/carnithine racemase n=2 Tax=Th... [more]
A0A661BRA8_9GAMM1.280e-1048.65Uncharacterized protein n=1 Tax=Gammaproteobacteri... [more]
A0A850KCM8_9HYPH3.370e-1043.42Enoyl-CoA hydratase/isomerase family protein n=1 T... [more]
A0A241WAA0_9GAMM4.560e-1042.67Enoyl-CoA hydratase n=1 Tax=Acinetobacter sp. ANC ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1024contigH-akashiwo_Contig1024:268789..269535 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1385510.N781_03140
PFAMsECH_1
Model size238
Max annot lvl91061|Bacilli
Hectar predicted targeting categoryother localisation
Exons3
Evalue1.03e-12
EggNOG OGsCOG1024@1|root,COG1024@2|Bacteria,1TQ89@1239|Firmicutes,4HCH3@91061|Bacilli,2Y8VJ@289201|Pontibacillus
DescriptionCatalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA
Cds size234
COG categoryI
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387054.9637187-CDS-H-akashiwo_Contig1024:268788..2688661681387054.9637187-CDS-H-akashiwo_Contig1024:268788..268866Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1024 268789..268866 -
1681387054.973314-CDS-H-akashiwo_Contig1024:269130..2692171681387054.973314-CDS-H-akashiwo_Contig1024:269130..269217Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1024 269131..269217 -
1681387054.9818614-CDS-H-akashiwo_Contig1024:269462..2695311681387054.9818614-CDS-H-akashiwo_Contig1024:269462..269531Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1024 269463..269531 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387054.9911487-UTR-H-akashiwo_Contig1024:269531..2695351681387054.9911487-UTR-H-akashiwo_Contig1024:269531..269535Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1024 269532..269535 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1024.5.1prot_H-akashiwo_Contig1024.5.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1024 268789..269531 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1024.5.1

>prot_H-akashiwo_Contig1024.5.1 ID=prot_H-akashiwo_Contig1024.5.1|Name=mRNA_H-akashiwo_Contig1024.5.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=78bp
MQLRCLILTGSTDKSFSVGRDLKISHLHETSERRLKYLKLGGQAVQAIHD
LEVPTIACVEGQCTGWGLELALACDLR*
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mRNA from alignment at H-akashiwo_Contig1024:268789..269535-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1024.5.1 ID=mRNA_H-akashiwo_Contig1024.5.1|Name=mRNA_H-akashiwo_Contig1024.5.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=747bp|location=Sequence derived from alignment at H-akashiwo_Contig1024:268789..269535- (Heterosigma akashiwo CCMP452)
TCCAATGCAACTTCGCTGCCTCATTTTAACTGGAAGCACTGACAAGTCGT TCAGTGTTGGCCGGGACCTGAAGGTGCCTCCAGTGCACCATGGTGATGAT GAAATAATATATTATATAATAATTCGGATGTGCAGTTGTAAAGCTTTTCC TTTTCAGTACCACGTCCTCCTTTTGAACTATCATAAATGTGACTCAACAT CAAAATGAAGAATGATACATGCCATGATAATTCATATCATAACTATACAA CAATAATACCACCACACTCTTCTTGACGGCCTGCTAACAACAAGAATACT ATGCTAAAAAAAAAAAAGATAAGCCATCTACATGAGACTTCTGAGCGCCG CCTGAAGTACCTAAAATTGGGAGGTCAAGCTGTTCAGGCAATACATGACC TTGAAGTAAGGATTAACTGAAGTGACCATGGTTCTGGTTTTTTTTTTTTT GTGAATTTTCTATCTATCTAGATGCGATGAATAAAAAAGCGTAATGATAA TATTACTTACTATCTAGATTAAATATGAAATAAATTAATATATTATTATT ATGTGGTAGTAGCAAATCAATATATACTAAAATTAATATATTATTATTAT GTAGTAGTAGCAAATCAATCTATACTTCAATGGTTTTCTATCTCGCTCAC TTATACCCCCTTCAACCAGGTTCCAACCATTGCCTGTGTCGAGGGACAGT GTACTGGATGGGGGCTAGAATTGGCTCTGGCTTGTGATCTGAGGTGA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1024:268789..269535-

>mRNA_H-akashiwo_Contig1024.5.1 ID=mRNA_H-akashiwo_Contig1024.5.1|Name=mRNA_H-akashiwo_Contig1024.5.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=234bp|location=Sequence derived from alignment at H-akashiwo_Contig1024:268789..269535- (Heterosigma akashiwo CCMP452)
ATGCAACTTCGCTGCCTCATTTTAACTGGAAGCACTGACAAGTCGTTCAG
TGTTGGCCGGGACCTGAAGATAAGCCATCTACATGAGACTTCTGAGCGCC
GCCTGAAGTACCTAAAATTGGGAGGTCAAGCTGTTCAGGCAATACATGAC
CTTGAAGTTCCAACCATTGCCTGTGTCGAGGGACAGTGTACTGGATGGGG
GCTAGAATTGGCTCTGGCTTGTGATCTGAGGTGA
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