mRNA_H-akashiwo_Contig1007.9.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1007.9.1
Unique NamemRNA_H-akashiwo_Contig1007.9.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A7S3UVC1_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UVC1_HETAK)

HSP 1 Score: 254 bits (650), Expect = 7.080e-84
Identity = 130/141 (92.20%), Postives = 130/141 (92.20%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDRDRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFP 423
            LIGAL WLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYA QHLDEL    GG GLEIRGY DADFA DRDRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKE LHLRQLCGFLLLLLQ DQFP
Sbjct:   34 LIGALTWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYAHQHLDELXXXXGGGGLEIRGYADADFARDRDRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKETLHLRQLCGFLLLLLQLDQFP 174          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: H3GB69_PHYRM (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GB69_PHYRM)

HSP 1 Score: 123 bits (309), Expect = 9.880e-31
Identity = 80/202 (39.60%), Postives = 116/202 (57.43%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDRD-RKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            L+GAL +L  CT P +A AV  +A+F     + HW AA  +L Y +   D   +  GG   E+ GY DA++A D D R+ST+GYVFF+  + +SW S++Q  +   ST AE  AL  A +EA+ LRQL    LL +  +    T   QDN  C+ LA+     S TKHID+K  ++++++ +  IVL    T+ M+AD  TK
Sbjct:   97 LVGALMYLATCTRPDIAQAVGEVAKFCERYNKSHWVAAKRILKYLKTTQDLGLVFNGGTKGELIGYADANWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNATQEAVWLRQL----LLDVGCECVGATTFFQDNQGCIALAKNPAYHSRTKHIDLKYHFLREKVEDKVIVLEYKPTDEMIADGFTK 294          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: H3GB17_PHYRM (Reverse transcriptase Ty1/copia-type domain-containing protein n=2 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GB17_PHYRM)

HSP 1 Score: 124 bits (310), Expect = 3.780e-30
Identity = 80/202 (39.60%), Postives = 115/202 (56.93%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDRD-RKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            L+GAL +L  CT P +A AV  +A+F     + HW AA  +L Y +   D   +  GG   E+ GY DA +A D D R+ST+GYVFF+  + +SW S++Q  +   ST AE  AL  A +EA+ LRQL    LL +  +    T   QDN  C+ LA+     S TKHID+K  ++++++ +  IVL    T+ M+AD  TK
Sbjct:  193 LVGALMYLATCTRPDIAQAVGEVAKFCERYNKSHWVAAKRILKYLKTTQDLGLVFNGGTKGELIGYADASWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNATQEAVWLRQL----LLDVGCECVAATTIFQDNQGCIALAKNPAYHSRTKHIDLKYHFLREKVEDKVIVLEYKPTDEMIADGFTK 390          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A2P4XPH2_9STRA (RxLR effector candidate protein (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4XPH2_9STRA)

HSP 1 Score: 116 bits (290), Expect = 1.300e-28
Identity = 77/202 (38.12%), Postives = 113/202 (55.94%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDRD-RKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            L+GAL +L   T P +A AV  +A+F     + HW AA  +L Y +   D   +  GG   E+ GY DA++A D D R+ST+GYVFF+  + +SW S++Q  +   ST AE  AL  A +EA+ LRQL    L  L  +    T  +QDN  C+ LA+     + TKHIDIK  ++++++    + L    T+ M+AD  TK
Sbjct:   25 LVGALMYLATSTRPNIAYAVGEVAKFCERYNKSHWVAAKRILKYLKTTQDVGLVFNGGIKGELVGYADANWAGDLDTRRSTTGYVFFLNGSAISWKSKRQPTVATSSTEAEYMALYNAIQEAVWLRQL----LKDLGYENKGATTIYQDNQGCIALAKNPAYHTRTKHIDIKFHFLREKVESKTVELEYKPTDEMIADGFTK 222          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A085LNL9_9BILA (Uncharacterized protein n=2 Tax=Trichuris suis TaxID=68888 RepID=A0A085LNL9_9BILA)

HSP 1 Score: 117 bits (292), Expect = 1.450e-28
Identity = 76/202 (37.62%), Postives = 117/202 (57.92%), Query Frame = 1
Query:    4 IGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRG-LEIRGYVDADFALDR-DRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            +G+L +L   + P +A AV +L + + +PT+  W A   V+ Y    + + +L     G +++ G+VDAD+A D  DRKSTSGYVF IGH+T++W+S+KQ  + + ST AE  A  EA +E   LRQL    L  ++  Q  PT  ++DN  C+ +A+  + S  TKHID++   ++       + LV   +  M ADVLTK
Sbjct:   62 VGSLLYLATVSRPDIAFAVGQLCRRVESPTQSDWKAVKRVIRYLAGSVSQ-KLRFSSEGNIKLTGFVDADWAGDHADRKSTSGYVFQIGHSTIAWSSKKQSVVAMSSTEAEYVAASEACREVQWLRQL----LADMEVPQDGPTKIYEDNQGCIKVAQSDRCSQRTKHIDVRHHQLRDLCESGIVSLVYCPSSEMPADVLTK 258          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A4Y1ZX26_ARAVE (Retrovirus-related Pol polyprotein from transposon TNT 1-94 n=2 Tax=Araneus ventricosus TaxID=182803 RepID=A0A4Y1ZX26_ARAVE)

HSP 1 Score: 115 bits (287), Expect = 7.140e-28
Identity = 76/203 (37.44%), Postives = 118/203 (58.13%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDE-LELEIGGRGLEIRGYVDADFA-LDRDRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            LIG+L +L  CT P +A ++++L+Q+L    + HW AA  VL Y ++ ++  L  E+  +   + GY D+D+    +DRKS   Y F + ++ +SW SRKQK + L ST +E  +L ++ KEAL+L++L   L L +   Q    +   DN S + LAE     S TKHIDI+  +I+  + + KI +  V T  M AD+LTK
Sbjct:   51 LIGSLNYLAVCTRPDIAYSISKLSQYLTCYGKSHWLAAERVLRYLKKTINYGLVFELDDK--VVYGYSDSDWGNYQKDRKSYFSYYFMLSNSVISWKSRKQKTVALSSTESEYMSLSDSCKEALYLQKLLSELDLGILYKQITINV---DNNSAIKLAENSLFHSRTKHIDIRQHFIRDVLEDGKIAVKHVSTSRMGADILTK 248          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A0P6IXK4_AEDAE (Putative copia-3 dwil-i (Fragment) n=1 Tax=Aedes aegypti TaxID=7159 RepID=A0A0P6IXK4_AEDAE)

HSP 1 Score: 119 bits (299), Expect = 1.050e-27
Identity = 75/202 (37.13%), Postives = 119/202 (58.91%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALD-RDRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            L+GAL +++    P +A A + LA+ +  PTE  W AA  V+ Y R  L+ + L+  G    + GY DAD++ D + RKSTSG+VF +G T ++W S++Q  + L S  +E  AL EA +E L LR+L    L  L  +Q  PT+ H+DN SC+      ++   +KHI+ K+++++    + +I L  + +E M+AD LTK
Sbjct: 1100 LVGALLYISVNARPDIAVATSILARKVSQPTEADWTAAKRVVRYLRGTLNWI-LQFSGSAPTLMGYSDADWSGDHKTRKSTSGFVFTVGGTAVAWRSKQQSIVALSSMESEYVALSEATQEVLWLRRL----LADLGFEQKEPTVLHEDNQSCIAFVRSERVGKRSKHIETKEMFVRNLCEKGEITLQYLCSEEMIADALTK 1296          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: UPI001781516D (secreted RxLR effector protein 161-like n=1 Tax=Rhagoletis pomonella TaxID=28610 RepID=UPI001781516D)

HSP 1 Score: 114 bits (284), Expect = 1.420e-27
Identity = 75/205 (36.59%), Postives = 113/205 (55.12%), Query Frame = 1
Query:    4 IGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDRD-RKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPT----ITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            IG L +    T P ++ AVN L++F   P + HWAA   V+ Y +  + +  +        + G+ D+D+A D D R+ST+GY+F +    +SW +R+Q+ I L ST AE  A+  A +EA  L +L        Q++  P T    + H DN S + +A     S  TKH+DIKD +I+Q +   KI LV + T+ MLAD+LTK
Sbjct:   41 IGCLLFAAQTTRPDISFAVNLLSRFSTNPGKAHWAAVKRVMRYLKGTI-KYGISYNKEACNLTGFCDSDWASDLDERRSTTGYLFKLQDGPISWCTRRQRTIALSSTEAEFMAMTSAIQEATWLMRL--------QKELTPDTAETLVLHCDNKSAIQVALNNSYSPRTKHVDIKDKFIRQHLESGKIKLVYIPTKEMLADILTK 236          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A085N351_9BILA (Reverse transcriptase Ty1/copia-type domain-containing protein n=1 Tax=Trichuris suis TaxID=68888 RepID=A0A085N351_9BILA)

HSP 1 Score: 117 bits (292), Expect = 2.470e-27
Identity = 77/202 (38.12%), Postives = 117/202 (57.92%), Query Frame = 1
Query:    4 IGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRG-LEIRGYVDADFALDR-DRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            +G+L +L   + P +A AV +L + + +PT+  W A   V+ Y    + + +L     G +++ G+VDAD+A D  DRKSTSGYVF IGH+T++W+S+KQ  + + ST AE  A  EA +E   LRQL    L  ++  Q  PT T++DN  C+ +A+  + S  TKHID+    ++       + LV   +  M ADVLTK
Sbjct:  240 VGSLLYLATVSRPDIAFAVGQLCRRVESPTQSDWKAVKRVIRYLAGSVCQ-KLRFSSEGNIKLTGFVDADWAGDHADRKSTSGYVFQIGHSTIAWSSKKQSVVAMSSTEAEYAAASEACRELQWLRQL----LADMEVPQDGPTRTYEDNQGCIKVAQSDRCSQRTKHIDVSHHQLRDLCESGILSLVYCPSSEMPADVLTK 436          
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Match: A0A0A9Y059_LYGHE (Copia protein (Fragment) n=1 Tax=Lygus hesperus TaxID=30085 RepID=A0A0A9Y059_LYGHE)

HSP 1 Score: 117 bits (293), Expect = 2.540e-27
Identity = 77/205 (37.56%), Postives = 119/205 (58.05%), Query Frame = 1
Query:    1 LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLDELELEIGGRGLEIRGYVDADFALDR-DRKSTSGYVFFIGHTTLSWASRKQKGIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFP---PTITHQDNLSCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLTK 603
            LIG+L +L   T P ++ AV+ L+QF     + HW AA  VL Y  Q    + L+    G  + GY DAD+A  + DR+S +GY F   +  +SW SRKQ+ + L +THAE  AL +A KEA+HL+       LL++   +P   P I + DN S   L++   +SS +KHI +++ +I++ I + +I ++ V +  M AD+LTK
Sbjct:  273 LIGSLMYLAVATRPDISHAVSFLSQFNSCHGQEHWLAAKRVLRYL-QGTKHIGLKYSKHGRALHGYTDADWASCQIDRRSYTGYCFKYANAAISWESRKQRTVALATTHAEYMALTDAAKEAIHLK-------LLVKESGYPVTGPIIIYNDNESAENLSKNNVVSSRSKHIAVREHFIRETIQQGEIDVIHVPSGEMDADILTK 469          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1007.9.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3UVC1_HETAK7.080e-8492.20Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
H3GB69_PHYRM9.880e-3139.60Reverse transcriptase Ty1/copia-type domain-contai... [more]
H3GB17_PHYRM3.780e-3039.60Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A2P4XPH2_9STRA1.300e-2838.12RxLR effector candidate protein (Fragment) n=1 Tax... [more]
A0A085LNL9_9BILA1.450e-2837.62Uncharacterized protein n=2 Tax=Trichuris suis Tax... [more]
A0A4Y1ZX26_ARAVE7.140e-2837.44Retrovirus-related Pol polyprotein from transposon... [more]
A0A0P6IXK4_AEDAE1.050e-2737.13Putative copia-3 dwil-i (Fragment) n=1 Tax=Aedes a... [more]
UPI001781516D1.420e-2736.59secreted RxLR effector protein 161-like n=1 Tax=Rh... [more]
A0A085N351_9BILA2.470e-2738.12Reverse transcriptase Ty1/copia-type domain-contai... [more]
A0A0A9Y059_LYGHE2.540e-2737.56Copia protein (Fragment) n=1 Tax=Lygus hesperus Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1007contigH-akashiwo_Contig1007:251672..252274 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog5297.GMQ_23310T0
PFAMsDUF5314,RVT_2,gag_pre-integrs,rve,zf-CCHC
Model size603
Max annot lvl4751|Fungi
Hectar predicted targeting categorysignal peptide
Exons1
Evalue3.05e-30
EggNOG OGsCOG2801@1|root,KOG0017@2759|Eukaryota,38DPC@33154|Opisthokonta,3NUZ7@4751|Fungi,3UY7N@5204|Basidiomycota,2YC45@29000|Pucciniomycotina
DescriptionReverse transcriptase (RNA-dependent DNA polymerase)
Cds size603
COG categoryL
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387048.5260065-CDS-H-akashiwo_Contig1007:251671..2522741681387048.5260065-CDS-H-akashiwo_Contig1007:251671..252274Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1007 251672..252274 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1007.9.1prot_H-akashiwo_Contig1007.9.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1007 251672..252274 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1007.9.1

>prot_H-akashiwo_Contig1007.9.1 ID=prot_H-akashiwo_Contig1007.9.1|Name=mRNA_H-akashiwo_Contig1007.9.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=201bp
LIGALAWLTCCTAPTLATAVNRLAQFLVAPTELHWAAAIAVLAYARQHLD
ELELEIGGRGLEIRGYVDADFALDRDRKSTSGYVFFIGHTTLSWASRKQK
GIVLHSTHAELNALLEAGKEALHLRQLCGFLLLLLQRDQFPPTITHQDNL
SCVGLAEKGQLSSLTKHIDIKDLWIKQEIHEMKIVLVPVRTEHMLADVLT
K
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mRNA from alignment at H-akashiwo_Contig1007:251672..252274-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1007.9.1 ID=mRNA_H-akashiwo_Contig1007.9.1|Name=mRNA_H-akashiwo_Contig1007.9.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=603bp|location=Sequence derived from alignment at H-akashiwo_Contig1007:251672..252274- (Heterosigma akashiwo CCMP452)
CTGATTGGCGCACTCGCTTGGCTCACGTGTTGCACGGCGCCCACGCTTGC AACAGCAGTGAACAGGCTGGCGCAGTTTCTGGTTGCTCCAACGGAACTGC ATTGGGCGGCAGCGATCGCGGTCTTGGCCTATGCGCGCCAGCACCTGGAC GAGCTGGAGCTGGAGATCGGCGGCAGAGGCCTGGAGATCAGGGGATACGT GGACGCCGACTTTGCCCTGGACAGGGACCGCAAGTCTACCTCAGGGTATG TGTTCTTCATTGGGCACACCACCCTATCATGGGCTTCGAGGAAGCAAAAA GGGATTGTGCTCCACAGCACACACGCGGAGCTCAACGCCCTACTGGAGGC GGGTAAGGAGGCACTTCACCTGCGGCAGTTGTGTGGATTCTTACTGCTTC TGCTGCAGCGCGACCAGTTCCCGCCTACTATCACCCATCAGGATAATCTC AGCTGTGTGGGACTTGCTGAGAAAGGCCAGCTTTCCTCACTCACCAAGCA TATCGACATCAAAGACCTATGGATCAAGCAGGAGATCCATGAGATGAAGA TAGTGCTGGTGCCAGTGAGGACAGAGCACATGCTGGCAGATGTGCTTACC AAG
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1007:251672..252274-

>mRNA_H-akashiwo_Contig1007.9.1 ID=mRNA_H-akashiwo_Contig1007.9.1|Name=mRNA_H-akashiwo_Contig1007.9.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=603bp|location=Sequence derived from alignment at H-akashiwo_Contig1007:251672..252274- (Heterosigma akashiwo CCMP452)
CTGATTGGCGCACTCGCTTGGCTCACGTGTTGCACGGCGCCCACGCTTGC
AACAGCAGTGAACAGGCTGGCGCAGTTTCTGGTTGCTCCAACGGAACTGC
ATTGGGCGGCAGCGATCGCGGTCTTGGCCTATGCGCGCCAGCACCTGGAC
GAGCTGGAGCTGGAGATCGGCGGCAGAGGCCTGGAGATCAGGGGATACGT
GGACGCCGACTTTGCCCTGGACAGGGACCGCAAGTCTACCTCAGGGTATG
TGTTCTTCATTGGGCACACCACCCTATCATGGGCTTCGAGGAAGCAAAAA
GGGATTGTGCTCCACAGCACACACGCGGAGCTCAACGCCCTACTGGAGGC
GGGTAAGGAGGCACTTCACCTGCGGCAGTTGTGTGGATTCTTACTGCTTC
TGCTGCAGCGCGACCAGTTCCCGCCTACTATCACCCATCAGGATAATCTC
AGCTGTGTGGGACTTGCTGAGAAAGGCCAGCTTTCCTCACTCACCAAGCA
TATCGACATCAAAGACCTATGGATCAAGCAGGAGATCCATGAGATGAAGA
TAGTGCTGGTGCCAGTGAGGACAGAGCACATGCTGGCAGATGTGCTTACC
AAG
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