mRNA_H-akashiwo_Contig100.2.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig100.2.1
Unique NamemRNA_H-akashiwo_Contig100.2.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A7S2UTE4_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UTE4_9STRA)

HSP 1 Score: 101 bits (252), Expect = 2.550e-24
Identity = 50/107 (46.73%), Postives = 73/107 (68.22%), Query Frame = 1
Query:   40 QLGVAPRINGGLLSNFQGKYVTLVGKVLN--QNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLFY 354
            Q GV PRING L  NF G+ V LVGKVL+  +N   A  ++SDG  V V ++PG  Y + +VEI GEV  DL+++E KS +YG++F+++ Y  +++LA G++  LFY
Sbjct:    7 QEGVTPRINGSLRQNFVGRPVCLVGKVLDVQENSGSAVIQSSDGSSVQVVMAPGSSYLTSFVEIVGEVLPDLSIKEFKSIDYGENFDLDLYNQVLQLANGKYQKLFY 113          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: D7FVA2_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FVA2_ECTSI)

HSP 1 Score: 91.3 bits (225), Expect = 2.620e-20
Identity = 47/102 (46.08%), Postives = 67/102 (65.69%), Query Frame = 1
Query:   55 PRINGGLLSNFQGKYVTLVGKVLN-QNGQI-ATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLFY 354
            PR+NG +LS F G  V +VGKVL+ Q G   A  E SDG ++TVQ++ G  + SQYVE+ G ++    ++E KS ++GD F++  Y+  V L TG+FS LFY
Sbjct:    7 PRVNGAVLSQFLGNTVCVVGKVLSYQEGDSEAQLETSDGAKITVQMTAGSTWTSQYVEVIGHLHEPDRLQEFKSTDFGDMFDLRKYDEAVSLMTGKFSYLFY 108          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A6G0XXL0_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XXL0_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.270e-19
Identity = 43/100 (43.00%), Postives = 65/100 (65.00%), Query Frame = 1
Query:   52 APRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLF 351
            APR+N  +++ F G+ V LVG + +  G +A  + SDG  VTV   PG  Y+S++VE+ G V  D +++E K   +GD F+++NY+ LV L  G+FSSLF
Sbjct:    7 APRVNKAIMAQFVGRTVALVGSLESWTGSMAVVKTSDGGLVTVHPQPGADYSSKFVEVIGLVQPDGSLKEYKCTSFGDDFDLKNYDKLVHLIHGKFSSLF 106          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A024UW39_9STRA (Uncharacterized protein n=3 Tax=Aphanomyces TaxID=100860 RepID=A0A024UW39_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 5.020e-19
Identity = 42/100 (42.00%), Postives = 64/100 (64.00%), Query Frame = 1
Query:   52 APRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLF 351
            APR+N  +L+ F G  V+LVG + +  G  A  + SD   VTV   PG  Y+S+YVE+ G V  D +++E K   +GD F+++NY+ LV+L  G++S+LF
Sbjct:    7 APRVNKAILAQFVGHTVSLVGSIESWTGSQAVLKTSDNGFVTVHPQPGADYSSKYVEVIGSVQPDGSLKEFKCTSFGDDFDLKNYDKLVQLIHGKYSTLF 106          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A7S2DZE6_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2DZE6_9STRA)

HSP 1 Score: 79.7 bits (195), Expect = 1.000e-15
Identity = 44/116 (37.93%), Postives = 63/116 (54.31%), Query Frame = 1
Query:   19 NFQMDTKQLGVAPRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEV-----NSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLF 351
            N  MD   +GV+PR+N GLL  F GK V +VG+VL+Q+      E SDG    V  S GQ      +E+ G       +  +++R  + C   D+F+++NYEGLV L  G+   LF
Sbjct:    8 NHTMDG--VGVSPRVNAGLLPQFDGKRVCIVGRVLSQDADRVRVECSDGGHTIVLSSDGQFNERSIIEVVGVAGDHANDGSMSLRSERICPLDDTFDLQNYEGLVNLVNGKARHLF 121          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A2G4SIE8_RHIZD (Replication factor A protein 3 n=2 Tax=Rhizopus TaxID=4842 RepID=A0A2G4SIE8_RHIZD)

HSP 1 Score: 75.5 bits (184), Expect = 2.580e-14
Identity = 35/82 (42.68%), Postives = 52/82 (63.41%), Query Frame = 1
Query:   55 PRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNME 300
            PR+N  L   + GK V LVGK+ + +G  A  EASD  QV V L+    + +QYVE+ G+V+ D T+RE K+   GD+F+++
Sbjct:    6 PRVNSALREKYVGKTVRLVGKITSFSGNTAVLEASDHGQVLVHLNGESQWGTQYVEVIGQVDQDFTIREFKTTNMGDNFDLD 87          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A197JHU3_9FUNG (Replication factor A protein 3 n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197JHU3_9FUNG)

HSP 1 Score: 75.5 bits (184), Expect = 2.580e-14
Identity = 39/103 (37.86%), Postives = 61/103 (59.22%), Query Frame = 1
Query:   43 LGVAPRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLF 351
            L    R+N  +L N  G+ V  VG+  ++   +ATF ASD   VTV ++    Y +++VE+ G VN D ++ E+ S  +GD FNME Y+ +V+ A  +F S+F
Sbjct:    3 LPTTKRVNSAMLQNCIGETVRFVGEFKSRQHPVATFLASDKGSVTVVMNDTSQYGTKFVEVIGTVNPDRSITEMISTNFGDDFNMETYDAMVRKAQ-KFPSVF 104          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A7S0XQA8_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S0XQA8_HEMAN)

HSP 1 Score: 72.0 bits (175), Expect = 1.100e-12
Identity = 43/104 (41.35%), Postives = 56/104 (53.85%), Query Frame = 1
Query:   52 APRINGGLLSNFQGKYVTLVGKVLNQ--NGQIATFEASDGQQVTVQLSPGQH--YNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLATGRFSSLF 351
            APRIN  LL    G  VT+VGKV      G+    EASDG  + V  S      Y S++VE+ G VNSD TV E +   +G+ F+++ Y  LV L+ G    LF
Sbjct:   28 APRINSTLLQQRVGMQVTVVGKVKPGFGGGEFVHLEASDGGDIVVNRSQAVQGEYGSEFVEVVGTVNSDRTVTEARFTNFGNDFDLKQYNDLVLLSNGALKHLF 131          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A1C7NGW9_9FUNG (Replication factor A protein 3 n=1 Tax=Choanephora cucurbitarum TaxID=101091 RepID=A0A1C7NGW9_9FUNG)

HSP 1 Score: 70.5 bits (171), Expect = 2.120e-12
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 1
Query:   55 PRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVK 321
            PRIN  L   + G+ V LVGK+ + +G  A  +ASD  QV V+L+    + + YVE+ G++ SD T+RE K+   G+SF+++    +V+
Sbjct:    6 PRINSALREKYVGRVVRLVGKLHSLSGNTAVLDASDHGQVMVKLNGESQWGTDYVEVIGQIESDYTLREFKTSNLGNSFDLDMANKVVE 94          
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Match: A0A433QMJ0_9FUNG (Uncharacterized protein n=2 Tax=Jimgerdemannia flammicorona TaxID=994334 RepID=A0A433QMJ0_9FUNG)

HSP 1 Score: 68.2 bits (165), Expect = 3.870e-11
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = 1
Query:   55 PRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVTVQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSF 291
            PRIN  L S + G+ V +V KV+  +G+IAT EA D  QV V ++    + + YVE+   V S+L++ EL S ++GDSF
Sbjct:   18 PRINSELRSQYCGQIVRIVSKVIRIDGKIATIEAPDSTQVQVTMADDSQWGTLYVELIARVESNLSLTELASTDFGDSF 96          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig100.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S2UTE4_9STRA2.550e-2446.73Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
D7FVA2_ECTSI2.620e-2046.08Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A6G0XXL0_9STRA1.270e-1943.00Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A024UW39_9STRA5.020e-1942.00Uncharacterized protein n=3 Tax=Aphanomyces TaxID=... [more]
A0A7S2DZE6_9STRA1.000e-1537.93Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A2G4SIE8_RHIZD2.580e-1442.68Replication factor A protein 3 n=2 Tax=Rhizopus Ta... [more]
A0A197JHU3_9FUNG2.580e-1437.86Replication factor A protein 3 n=1 Tax=Linnemannia... [more]
A0A7S0XQA8_HEMAN1.100e-1241.35Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
A0A1C7NGW9_9FUNG2.120e-1238.20Replication factor A protein 3 n=1 Tax=Choanephora... [more]
A0A433QMJ0_9FUNG3.870e-1143.04Uncharacterized protein n=2 Tax=Jimgerdemannia fla... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig100contigH-akashiwo_Contig100:28283..29273 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FVA2
PFAMsRep_fac-A_3
Model size620
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons4
Evalue2.07e-22
EggNOG OGs2C2US@1|root,2S5GF@2759|Eukaryota
Ec32 ortholog descriptionReplication factor A protein 3
Ec32 orthologEc-14_000330.1
DescriptionReplication factor A protein 3
Cds size330
COG categoryS
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig100.2.1prot_H-akashiwo_Contig100.2.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig100 28546..29246 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387046.5831592-UTR-H-akashiwo_Contig100:28282..285451681387046.5831592-UTR-H-akashiwo_Contig100:28282..28545Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig100 28283..28545 -
1681387046.6326-UTR-H-akashiwo_Contig100:29246..292731681387046.6326-UTR-H-akashiwo_Contig100:29246..29273Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig100 29247..29273 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387046.593872-CDS-H-akashiwo_Contig100:28545..286101681387046.593872-CDS-H-akashiwo_Contig100:28545..28610Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig100 28546..28610 -
1681387046.603012-CDS-H-akashiwo_Contig100:28728..288111681387046.603012-CDS-H-akashiwo_Contig100:28728..28811Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig100 28729..28811 -
1681387046.6133204-CDS-H-akashiwo_Contig100:28942..289921681387046.6133204-CDS-H-akashiwo_Contig100:28942..28992Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig100 28943..28992 -
1681387046.6230922-CDS-H-akashiwo_Contig100:29114..292461681387046.6230922-CDS-H-akashiwo_Contig100:29114..29246Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig100 29115..29246 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig100.2.1

>prot_H-akashiwo_Contig100.2.1 ID=prot_H-akashiwo_Contig100.2.1|Name=mRNA_H-akashiwo_Contig100.2.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=110bp
MDTKQLGVAPRINGGLLSNFQGKYVTLVGKVLNQNGQIATFEASDGQQVT
VQLSPGQHYNSQYVEITGEVNSDLTVRELKSCEYGDSFNMENYEGLVKLA
TGRFSSLFY*
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mRNA from alignment at H-akashiwo_Contig100:28283..29273-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig100.2.1 ID=mRNA_H-akashiwo_Contig100.2.1|Name=mRNA_H-akashiwo_Contig100.2.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=991bp|location=Sequence derived from alignment at H-akashiwo_Contig100:28283..29273- (Heterosigma akashiwo CCMP452)
CGCTTTTGTATTATATAAAACTTTCAAATGGACACAAAGCAGCTAGGCGT TGCCCCTCGCATTAATGGAGGGCTGCTTTCAAATTTTCAGGGAAAATATG TTACATTGGTCGGCAAAGTGCTAAATCAAAATGGCCAAATAGCAACTTTT GAAGCTTCGGTAAACTCGATCTTCTGCACGTACTTGTCCTTTCTGTCTAC CTCCTGGATATGATTTCTTTCGTTTCAGTTCGCACAACTCGAGAGACCCT CGTTGTCTATTGCTATCTTATGAAATTCCAGGATGGGCAACAAGTTACAG TGCAGTTAAGTCCGGGTCAACACTACAACAGGTACGCTGCCGCAGCAGTG CATTTGAATGCTGCAATTTTTTTAGTATTTTTCACACATGCCCACTTTCT CCTCATTTTTGTGTGAAACCAGCCCTCTCCGCCTCAATTTTTGACTTCGA ATTCTGCTGCAGCCAATATGTGGAAATCACTGGGGAAGTGAACTCAGACT TGACAGTTCGGGAACTCAAGAGCTGTGAATATGGGGATTCTTTCAGTGAG CCTTTTCTGCACCAAATGATGTGTAACACAAGAACTTTTGTGCTTTTCAA AACATAGTCATCCAACTTTCACTGATAAATCTATTCGGAATCTCCAAATA AATTTTACTGCAGACATGGAAAACTACGAAGGTCTGGTAAAACTTGCAAC CGGAAGATTCAGTAGTCTATTCTACTAAAAAACAACAACAACAACAAAAA GCAACAACACAAGAGCTTCCGCATGAAAATGTGTTATATCAGCAGGCCAT GGTTGTACTCAGGGTACAATTCGATGGACGTGAGGACGGACCGGTGGCCA TTAGAGCATGGCACAAAGGAGGGTCTTATTGTGATCTTCTCCAGTTGGTG GAATATTTGTATTTTATTACATATTTATTATTATACTTATAATTATGATG ATGATGATGATGTTATTTTTAGTTTCAAGTCTAACTTTTCA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig100:28283..29273-

>mRNA_H-akashiwo_Contig100.2.1 ID=mRNA_H-akashiwo_Contig100.2.1|Name=mRNA_H-akashiwo_Contig100.2.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=330bp|location=Sequence derived from alignment at H-akashiwo_Contig100:28283..29273- (Heterosigma akashiwo CCMP452)
ATGGACACAAAGCAGCTAGGCGTTGCCCCTCGCATTAATGGAGGGCTGCT
TTCAAATTTTCAGGGAAAATATGTTACATTGGTCGGCAAAGTGCTAAATC
AAAATGGCCAAATAGCAACTTTTGAAGCTTCGGATGGGCAACAAGTTACA
GTGCAGTTAAGTCCGGGTCAACACTACAACAGCCAATATGTGGAAATCAC
TGGGGAAGTGAACTCAGACTTGACAGTTCGGGAACTCAAGAGCTGTGAAT
ATGGGGATTCTTTCAACATGGAAAACTACGAAGGTCTGGTAAAACTTGCA
ACCGGAAGATTCAGTAGTCTATTCTACTAA
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