mRNA_H-canaliculatus_F_contig13202.2638.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig13202.2638.1
Unique NamemRNA_H-canaliculatus_F_contig13202.2638.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
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BLAST of mRNA_H-canaliculatus_F_contig13202.2638.1 vs. uniprot
Match: A0A6H5KAS6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KAS6_9PHAE)

HSP 1 Score: 326 bits (835), Expect = 5.270e-97
Identity = 225/402 (55.97%), Postives = 263/402 (65.42%), Query Frame = 1 
Query:    4 SAAAGAVLKGLGEAGGALMSMRDSEIARLRRAYARSELSREEMKRCLRALAVQLREGEDRTGALLAEREAALHEIGVKEAELAKKETLLQEGKARATVEVQEKEELVSSLKVENQALQAALERLRHDFQEERALRLRHDRRSSPATR------QRSQPSTPARLGIMGRMSLASRHGTPPSAAALAAAPAPAPGARGLEARGARRSEGGVGTRPPPKTPTRSGEMAGPELHNHLARSTPHRAGRASLGRGGR-PGGAPTVAAIA-----GWDSNHGGIGSRHNYPVLSAAGRAGSGGES--EEEERGQDRQGLPWILNTDQIWVGAVRPPHEVLVHLLDELEDLKVTHGGGSGSAEDLGKGFVAETEAFVEWSHRTGELQRVPPLESVDLPGEKKFTEGCLR 1167
            S AAGAVLKGLGEAGGALMSMRDSEI+RLRRAYARSELSREEMKRCLRALAVQLREGEDRTGAL+A+REAAL ++  KE E AKKE +L+EGKARAT EVQ++EELV+SL+VEN+ALQAALERLR DFQEER+LRL+    S PA         + +PSTP+RLGI  R  L SR G                G  G  A     S  G   RPP      S  ++G    NH  RSTP R G+     GGR  GG+  +AA A     GW S H GIGSRH+    + A  AG G E+  +E    Q  +GLPWILNTDQ           VL HLL+ELE +K   G      E+  +G +AET AF+EWSHRTGELQ +PPLE+VD+PGE+ FTEGCL+
Sbjct: 1059 STAAGAVLKGLGEAGGALMSMRDSEISRLRRAYARSELSREEMKRCLRALAVQLREGEDRTGALMADREAALCKLSAKEEEFAKKEAVLEEGKARATAEVQQQEELVTSLQVENEALQAALERLRLDFQEERSLRLQ----SGPAAAXXXXXXSKVRPSTPSRLGIFRRRGLTSRDG----GGTXXXXXXXXXGEEGQPA-----SRHGSVARPPATPSRHSRLLSGSSFLNHHIRSTPGRPGQ-----GGRLAGGSGAIAAAATPGGVGWSSVHRGIGSRHS----TTAAAAGGGSETWVQEVAGAQAVEGLPWILNTDQ-----------VLNHLLEELEGIKAMSG------EEKEEGSIAETAAFIEWSHRTGELQELPPLETVDMPGERAFTEGCLK 1421
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BLAST of mRNA_H-canaliculatus_F_contig13202.2638.1 vs. uniprot
Match: D7FK06_ECTSI (CRAL/TRIO domain containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FK06_ECTSI)

HSP 1 Score: 242 bits (617), Expect = 1.270e-67
Identity = 174/389 (44.73%), Postives = 202/389 (51.93%), Query Frame = 1 
Query:    4 SAAAGAVLKGLGEAGGALMSMRDSEIARLRRAYARSELSREEMKRCLRALAVQLREGEDRTGALLAEREAALHEIGVKEAELAKKETLLQEGKARATVEVQEKEELVSSLKVENQALQAALERLRHDFQEERALRLRHDRRSSPATRQRSQPSTPARLGIMGRMSLASRHGTPPSAAALAAAPAPAPGARGLEARGARRSEGGVGTRPPPKTPTRSGEMAGPELHNHLARSTPHRAGRASLGRGGRPGGAPTVAAIAGWDSNHGGIGSRHNYPVLSAAGRAGSGGESEEEERGQDRQGLPWILNTDQIWVGAVRPPHEVLVHLLDELEDLKVTHGGGSGSAEDLGKGFVAETEAFVEWSHRTGELQRVPPLESVDLPGEKKFTEGCLRV 1170
            S AAGAVLKGLGEAGGALMSMRDSEI+RLRRAYARSELSREEMKRCLRALAVQLREGEDRTGALLA+REAAL ++  KE E  KKE +L+EGKARAT EVQ+KEELV+SL+VEN+ALQAALERLR DFQEER+LRL+                                    P+AAA AAA                                                                       + +  W +                             ER              + W    RPPHEVL HLL+ELE +K   GG      +  +G +AET AF+EWSHRTGELQ VPPLE+VD+PGE+ FTEGCL++
Sbjct:  999 STAAGAVLKGLGEAGGALMSMRDSEISRLRRAYARSELSREEMKRCLRALAVQLREGEDRTGALLADREAALCKLNAKEEEFTKKEAVLEEGKARATAEVQQKEELVTSLQVENEALQAALERLRLDFQEERSLRLQSG----------------------------------PAAAAAAAA-----------------------------------------------------------------------SKVDTWKAR---------------------------AER--------------EAWADGARPPHEVLNHLLEELEGIKAMSGG------EQEEGSIAETAAFIEWSHRTGELQEVPPLETVDMPGERAFTEGCLKL 1235
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig13202.2638.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5KAS6_9PHAE5.270e-9755.97Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FK06_ECTSI1.270e-6744.73CRAL/TRIO domain containing protein n=1 Tax=Ectoca... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig13202contigH-canaliculatus_F_contig13202:465..3170 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score246.1
Seed ortholog evalue2.7e-62
Seed eggNOG ortholog2880.D7FK06
KEGG koko:K15335,ko:K19625
EggNOG free text desc.macromolecule localization
EggNOG OGsKOG1471@1,KOG1471@2759
EC2.1.1.203
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Hectar predicted targeting categoryother localisation
Exons5
Model size1170
Cds size1170
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig13202.2638.1prot_H-canaliculatus_F_contig13202.2638.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig13202 465..3170 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929059.6469016-CDS-H-canaliculatus_F_contig13202:464..6311622929059.6469016-CDS-H-canaliculatus_F_contig13202:464..631Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 465..631 +
1696845664.097718-CDS-H-canaliculatus_F_contig13202:464..6311696845664.097718-CDS-H-canaliculatus_F_contig13202:464..631Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 465..631 +
1622929059.663538-CDS-H-canaliculatus_F_contig13202:1151..12961622929059.663538-CDS-H-canaliculatus_F_contig13202:1151..1296Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 1152..1296 +
1696845664.108664-CDS-H-canaliculatus_F_contig13202:1151..12961696845664.108664-CDS-H-canaliculatus_F_contig13202:1151..1296Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 1152..1296 +
1622929059.6779976-CDS-H-canaliculatus_F_contig13202:1794..24061622929059.6779976-CDS-H-canaliculatus_F_contig13202:1794..2406Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 1795..2406 +
1696845664.125753-CDS-H-canaliculatus_F_contig13202:1794..24061696845664.125753-CDS-H-canaliculatus_F_contig13202:1794..2406Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 1795..2406 +
1622929059.6884792-CDS-H-canaliculatus_F_contig13202:2855..29651622929059.6884792-CDS-H-canaliculatus_F_contig13202:2855..2965Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 2856..2965 +
1696845664.1385376-CDS-H-canaliculatus_F_contig13202:2855..29651696845664.1385376-CDS-H-canaliculatus_F_contig13202:2855..2965Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 2856..2965 +
1622929059.7021775-CDS-H-canaliculatus_F_contig13202:3034..31701622929059.7021775-CDS-H-canaliculatus_F_contig13202:3034..3170Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 3035..3170 +
1696845664.1482081-CDS-H-canaliculatus_F_contig13202:3034..31701696845664.1482081-CDS-H-canaliculatus_F_contig13202:3034..3170Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig13202 3035..3170 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig13202.2638.1

>prot_H-canaliculatus_F_contig13202.2638.1 ID=prot_H-canaliculatus_F_contig13202.2638.1|Name=mRNA_H-canaliculatus_F_contig13202.2638.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=390bp
SSAAAGAVLKGLGEAGGALMSMRDSEIARLRRAYARSELSREEMKRCLRA
LAVQLREGEDRTGALLAEREAALHEIGVKEAELAKKETLLQEGKARATVE
VQEKEELVSSLKVENQALQAALERLRHDFQEERALRLRHDRRSSPATRQR
SQPSTPARLGIMGRMSLASRHGTPPSAAALAAAPAPAPGARGLEARGARR
SEGGVGTRPPPKTPTRSGEMAGPELHNHLARSTPHRAGRASLGRGGRPGG
APTVAAIAGWDSNHGGIGSRHNYPVLSAAGRAGSGGESEEEERGQDRQGL
PWILNTDQIWVGAVRPPHEVLVHLLDELEDLKVTHGGGSGSAEDLGKGFV
AETEAFVEWSHRTGELQRVPPLESVDLPGEKKFTEGCLRV
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mRNA from alignment at H-canaliculatus_F_contig13202:465..3170+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig13202.2638.1 ID=mRNA_H-canaliculatus_F_contig13202.2638.1|Name=mRNA_H-canaliculatus_F_contig13202.2638.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=2706bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13202:465..3170+ (Hapterophycus canaliculatus Oshoro5f female)
TCCTCCGCGGCGGCGGGGGCGGTGCTGAAGGGACTGGGGGAGGCCGGGGG
CGCCCTCATGTCCATGCGGGACTCGGAGATCGCGAGGCTGAGGAGGGCGT
ACGCCCGGTCGGAGCTGTCGAGGGAGGAGATGAAGCGCTGCCTGCGGGCG
CTGGCGGTGCAGCTGAGGTGTGTTTTGTCGGTTGGATTGGGTTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
CGTTTGTGTGTGTGGTCGTGAGTTTTGAGAAGGATTCGTCCGTGATCTTC
ATTCCCATCTTTTTGTGCGGCCGTGCACGGTGGTGGCGCTCGTGTTTGGC
TCCGTCTCGACAGCGTTTGCTGTCGGCGGCGCCTGAACGACGCCGTGGCG
TTGCCTGGGGGGGGGGAAATTCGCGGCGTTTCAGGACGGGGGCGGGCTCG
GTGGGAGGGCGCGAAGGGGGCAATAGCTAGGGGTTGATGTGGGCTGAGTA
GCGTGCCGCTCGTTGCCTGTGCCAGGGCGCTGGTGGCCCAATCGAGGTGC
TCTGTTCGACCCCTGTGCCTGCCCATCTCTCCCCCACGGCGGCCCCTACA
ACGTACTTGTACTGTTTTGTGTGGTCGATTCCGCCCCTCACTTCACCCCC
TTGTCCTGTGCTTCAACCTTTGTGCGCGTCTACTCAGGGAGGGAGAGGAT
AGGACGGGGGCGTTGCTGGCGGAGCGGGAAGCGGCGCTGCACGAAATCGG
CGTCAAGGAAGCGGAGCTTGCCAAGAAGGAGACGCTGCTCCAAGAGGGGA
AGGCCCGCGCGACCGTCGAGGTTCAGGAGAAGGTAAAACGTGTGTCTTGG
CTGTTGGTGTGTGTGTGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTTTGCTATCCGAGCTAGCCTGTTAG
ACGTCTGTGATTTTGCTATCCGAGCAAGAAGACGTCATTCCCTGCGAAAG
AAGCACGAAGAGACGAGGTGCTTCGGGAGATCTCCCATGAAGGTGGTGGT
TGGGGTGGGGGGGAGATATCTAGGTGCTGTGTGAGTGAATGAGCTTGCAC
GTATATCTGTCGATGCGTCATCGTCGGCGTCGGCGCCGGCGTCGGTGTTG
CTGTTGTCGTTGTCGTTGCTGTTGTTGTTGTTGTGTCACACCGACGATTC
TGCCGAACAGACGGGAAACCTTGCGCCTTCTTCGCAAGAGGAGCATTCTG
TTTTATACTTGCGGCAACAATTTCGTCGGAAAGAGAAACTCTCTAGCCCC
CCCCCCTCCCCCCGGCTTCCCCCCCCCCAGGAGGAGTTGGTATCGAGCCT
AAAGGTGGAGAACCAAGCGCTGCAGGCCGCCCTCGAGAGGCTGCGGCACG
ACTTCCAGGAGGAGAGAGCCCTGCGGCTCCGGCACGACAGAAGGAGTTCT
CCCGCCACCCGCCAGAGGTCCCAGCCGAGCACCCCTGCACGCCTCGGCAT
CATGGGAAGGATGAGCCTGGCGAGCCGCCACGGCACGCCGCCCTCTGCCG
CCGCTTTGGCGGCGGCGCCGGCGCCGGCGCCGGGAGCACGAGGGTTAGAG
GCGAGAGGGGCTCGGAGGTCTGAAGGAGGAGTGGGTACCCGGCCGCCGCC
GAAGACCCCCACTAGGAGCGGCGAAATGGCGGGGCCGGAGCTCCACAACC
ACCTGGCGCGGTCCACTCCGCATAGGGCGGGGCGCGCTTCGCTGGGGAGG
GGGGGGCGGCCGGGAGGCGCGCCCACCGTAGCCGCGATCGCGGGGTGGGA
TAGCAACCACGGCGGTATAGGTTCGAGGCACAACTATCCCGTGCTCTCGG
CGGCAGGGAGGGCCGGATCGGGGGGGGAGAGTGAGGAGGAGGAGAGAGGG
CAAGACCGCCAGGGACTGCCGTGGATTCTCAACACCGATCAGGTCCGTGC
TCGGTGAGCGGTGAGCTCCGTGCCCGACGCGGAAGGACGAAGAGAGGGGC
TCCCTCCTCTGTCGTTTGTGGGGACGGATAGATGGCGATGGCTGTGACTC
CCCTGCGCGAGGGAAGCAGGTGTTGCTCCCCTCCTTTCCACTCGCTCACG
CACGTTCGCCCGGCCGTCCCGCACACACACACACACACACACACACACAC
CGCTACCCTTTTGCCCGAAGAGCCTCTTTACGTTCAGGCAGGTGTGGCAA
GGGCGCTCTCTCTCTGAATGACCCACCTTTCCATCCTCACCTAGAGCGTG
AGCGCACGCATCCACCAATCCGTCCAGATCCGAACGATTAAAATGTCCTT
ACGAGCGCGACTGCGTGCGCGCTGTGACGCTACCGCCCCGACCGCCCCCT
CCCAAAGCCTGCTTAACCCCTCCTGCCCGCCCGTTCGCTAGATATGGGTC
GGCGCCGTCCGGCCGCCGCACGAGGTGCTAGTCCACCTCCTGGACGAGCT
GGAGGACCTCAAGGTTACCCACGGCGGGGGCAGCGGCTCGGCCGAGGACT
TGTGCGCAACTTCTCCACCGGAAGACAGCGGTGCAGAGGATGGGGGAAGT
CCGGGGCAGCGAAGAGGAAGAGGGAAGGGCTTTGTGGCGGAGACGGAGGC
GTTCGTGGAGTGGAGCCACCGCACCGGGGAGCTGCAGAGGGTGCCCCCGC
TCGAGTCGGTGGACCTCCCCGGCGAGAAGAAGTTCACGGAGGGGTGTCTG
CGGGTG
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig13202:465..3170+

>mRNA_H-canaliculatus_F_contig13202.2638.1 ID=mRNA_H-canaliculatus_F_contig13202.2638.1|Name=mRNA_H-canaliculatus_F_contig13202.2638.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=2340bp|location=Sequence derived from alignment at H-canaliculatus_F_contig13202:465..3170+ (Hapterophycus canaliculatus Oshoro5f female)
TCCTCCGCGGCGGCGGGGGCGGTGCTGAAGGGACTGGGGGAGGCCGGGGG
CGCCCTCATGTCCATGCGGGACTCGGAGATCGCGAGGCTGAGGAGGGCGT
ACGCCCGGTCGGAGCTGTCGAGGGAGGAGATGAAGCGCTGCCTGCGGGCG
CTGGCGGTGCAGCTGAGTCCTCCGCGGCGGCGGGGGCGGTGCTGAAGGGA
CTGGGGGAGGCCGGGGGCGCCCTCATGTCCATGCGGGACTCGGAGATCGC
GAGGCTGAGGAGGGCGTACGCCCGGTCGGAGCTGTCGAGGGAGGAGATGA
AGCGCTGCCTGCGGGCGCTGGCGGTGCAGCTGAGGGAGGGAGAGGATAGG
ACGGGGGCGTTGCTGGCGGAGCGGGAAGCGGCGCTGCACGAAATCGGCGT
CAAGGAAGCGGAGCTTGCCAAGAAGGAGACGCTGCTCCAAGAGGGGAAGG
CCCGCGCGACCGTCGAGGTTCAGGAGAAGGGAGGGAGAGGATAGGACGGG
GGCGTTGCTGGCGGAGCGGGAAGCGGCGCTGCACGAAATCGGCGTCAAGG
AAGCGGAGCTTGCCAAGAAGGAGACGCTGCTCCAAGAGGGGAAGGCCCGC
GCGACCGTCGAGGTTCAGGAGAAGGAGGAGTTGGTATCGAGCCTAAAGGT
GGAGAACCAAGCGCTGCAGGCCGCCCTCGAGAGGCTGCGGCACGACTTCC
AGGAGGAGAGAGCCCTGCGGCTCCGGCACGACAGAAGGAGTTCTCCCGCC
ACCCGCCAGAGGTCCCAGCCGAGCACCCCTGCACGCCTCGGCATCATGGG
AAGGATGAGCCTGGCGAGCCGCCACGGCACGCCGCCCTCTGCCGCCGCTT
TGGCGGCGGCGCCGGCGCCGGCGCCGGGAGCACGAGGGTTAGAGGCGAGA
GGGGCTCGGAGGTCTGAAGGAGGAGTGGGTACCCGGCCGCCGCCGAAGAC
CCCCACTAGGAGCGGCGAAATGGCGGGGCCGGAGCTCCACAACCACCTGG
CGCGGTCCACTCCGCATAGGGCGGGGCGCGCTTCGCTGGGGAGGGGGGGG
CGGCCGGGAGGCGCGCCCACCGTAGCCGCGATCGCGGGGTGGGATAGCAA
CCACGGCGGTATAGGTTCGAGGCACAACTATCCCGTGCTCTCGGCGGCAG
GGAGGGCCGGATCGGGGGGGGAGAGTGAGGAGGAGGAGAGAGGGCAAGAC
CGCCAGGGACTGCCGTGGATTCTCAACACCGATCAGGAGGAGTTGGTATC
GAGCCTAAAGGTGGAGAACCAAGCGCTGCAGGCCGCCCTCGAGAGGCTGC
GGCACGACTTCCAGGAGGAGAGAGCCCTGCGGCTCCGGCACGACAGAAGG
AGTTCTCCCGCCACCCGCCAGAGGTCCCAGCCGAGCACCCCTGCACGCCT
CGGCATCATGGGAAGGATGAGCCTGGCGAGCCGCCACGGCACGCCGCCCT
CTGCCGCCGCTTTGGCGGCGGCGCCGGCGCCGGCGCCGGGAGCACGAGGG
TTAGAGGCGAGAGGGGCTCGGAGGTCTGAAGGAGGAGTGGGTACCCGGCC
GCCGCCGAAGACCCCCACTAGGAGCGGCGAAATGGCGGGGCCGGAGCTCC
ACAACCACCTGGCGCGGTCCACTCCGCATAGGGCGGGGCGCGCTTCGCTG
GGGAGGGGGGGGCGGCCGGGAGGCGCGCCCACCGTAGCCGCGATCGCGGG
GTGGGATAGCAACCACGGCGGTATAGGTTCGAGGCACAACTATCCCGTGC
TCTCGGCGGCAGGGAGGGCCGGATCGGGGGGGGAGAGTGAGGAGGAGGAG
AGAGGGCAAGACCGCCAGGGACTGCCGTGGATTCTCAACACCGATCAGAT
ATGGGTCGGCGCCGTCCGGCCGCCGCACGAGGTGCTAGTCCACCTCCTGG
ACGAGCTGGAGGACCTCAAGGTTACCCACGGCGGGGGCAGCGGCTCGGCC
GAGGACTTATATGGGTCGGCGCCGTCCGGCCGCCGCACGAGGTGCTAGTC
CACCTCCTGGACGAGCTGGAGGACCTCAAGGTTACCCACGGCGGGGGCAG
CGGCTCGGCCGAGGACTTAGGGAAGGGCTTTGTGGCGGAGACGGAGGCGT
TCGTGGAGTGGAGCCACCGCACCGGGGAGCTGCAGAGGGTGCCCCCGCTC
GAGTCGGTGGACCTCCCCGGCGAGAAGAAGTTCACGGAGGGGTGTCTGCG
GGTGAGGGAAGGGCTTTGTGGCGGAGACGGAGGCGTTCGTGGAGTGGAGC
CACCGCACCGGGGAGCTGCAGAGGGTGCCCCCGCTCGAGTCGGTGGACCT
CCCCGGCGAGAAGAAGTTCACGGAGGGGTGTCTGCGGGTG
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