mRNA_H-canaliculatus_F_contig1387.3072.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig1387.3072.1
Unique NamemRNA_H-canaliculatus_F_contig1387.3072.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A6H5L132_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L132_9PHAE)

HSP 1 Score: 212 bits (539), Expect = 1.800e-66
Identity = 115/196 (58.67%), Postives = 135/196 (68.88%), Query Frame = 1 
Query:    1 RVGDVVERDIDGIWFPAKVLAVHERRFPTDSSGSCSNVWQPRQLAFDLVYLDDQNRESEVPEGDLRVVHDYGSRTKDNLLLLLREEDRSTSRAGEESRLGGGGSIGAGRRKTHTXXXXXXXXXXXSTHYDDRGRDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRGP 588
            RVG+VVERDIDG+WFPAKV+AVHE++     SG   ++ + RQLA+DL YLDD NRESEVPE D+RVV+DYG++TK+++LLLL  E       G +S    G S G G  K+              +   D G+D  V D      H GPKVTIHSS GTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRGP
Sbjct:   26 RVGEVVERDIDGVWFPAKVVAVHEKKDEAVPSGGSGSIGRRRQLAYDLFYLDDNNRESEVPEEDIRVVYDYGTKTKESMLLLLGTEHER----GGDSTAARGHSHGGGDSKSLDARQYVEAGANSRSRGQDGGKDGGVHDEDGDG-HAGPKVTIHSSLGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRGP 216
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: D8LHB0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHB0_ECTSI)

HSP 1 Score: 157 bits (397), Expect = 6.260e-46
Identity = 94/196 (47.96%), Postives = 105/196 (53.57%), Query Frame = 1 
Query:    1 RVGDVVERDIDGIWFPAKVLAVHERRFPTDSSGSCSNVWQPRQLAFDLVYLDDQNRESEVPEGDLRVVHDYGSRTKDNLLLLLREEDRSTSRAGEESRLGGGGSIGAGRRKTHTXXXXXXXXXXXSTHYDDRGRDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRGP 588
            RVG+VVERDIDG+WFPA+V+ VHE+ +    SGS  ++ +P QLA+DLVYLDD NRESE  EG    VHD G                                                          D G             H GPKVTIHSSRGTFDTKEDGSA+AYIINGPETNVAAGSGLRGIRWLRGP
Sbjct:   21 RVGEVVERDIDGVWFPAQVVGVHEKTYEAGRSGSSGSIGRPWQLAYDLVYLDDNNRESEG-EGKDGGVHDEG----------------------------------------------------------DDG-------------HAGPKVTIHSSRGTFDTKEDGSASAYIINGPETNVAAGSGLRGIRWLRGP 144
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A6S9KAU9_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6S9KAU9_HETAK)

HSP 1 Score: 86.7 bits (213), Expect = 5.610e-18
Identity = 62/194 (31.96%), Postives = 88/194 (45.36%), Query Frame = 1 
Query:    1 RVGDVVERDIDGIWFPAKVLAVHERRFPTDSSGSCSNVWQPRQLAFDLVYLDDQNRESEVPEGDLRVVHDYGSRTKDNLLLLLREEDRSTSRAGEESRLGGGGSIGAGRRKTHTXXXXXXXXXXXSTHYDDRGRDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLR 582
            +VG+VVERDIDG W+ AK+++V +  F               ++ +D+ Y+DD N E  VPE +LR        T +  L    +E  S +++             AGR +                  +++ R               P+V +H         +DG ATAYIINGPE NVA GSGLRGIRWL+
Sbjct:   20 QVGNVVERDIDGEWYAAKIVSVIQDEFD--------------EIIYDVEYVDDGNVEHGVPEDELRT---GDHETLEQALAKADDEGNSDNQSK------------AGRPREAPRPLGGALFPTRGEEEEEKRRQ------------AAPRVVVHG------LAQDGEATAYIINGPENNVATGSGLRGIRWLK 166
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A835Z3A3_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3A3_9STRA)

HSP 1 Score: 66.6 bits (161), Expect = 3.300e-10
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 1 
Query:  448 PKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRG 585
            P VT H+S    D ++DG A AYIINGP+TN+AAGSGLR +RWLRG
Sbjct:  148 PTVTYHTS-AAHDNRDDGQAGAYIINGPDTNIAAGSGLRALRWLRG 192
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A485KQE5_9STRA (Aste57867_10453 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KQE5_9STRA)

HSP 1 Score: 64.7 bits (156), Expect = 1.080e-9
Identity = 56/189 (29.63%), Postives = 78/189 (41.27%), Query Frame = 1 
Query:   16 VERDIDGIWFPAKVLAVHERRFPTDSSGSCSNVWQPRQLAFDLVYLDDQNRESEVPEGDLRVVHDYGSRTKDNLLLLLREEDRSTSRAGEESRLGGGGSIGAGRRKTHTXXXXXXXXXXXSTHYDDRGRDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLR 582
            VERDIDG+WFPA++LA H+                     +D+ Y +D   E  +P  +LR                 R+  R+ ++A        G S  A  R++                        L+Q       ++ P V +H+        E  +A  YIING ETNVAAG+GLRGIRWLR
Sbjct:   39 VERDIDGLWFPARILADHD------------------DGTYDVQYDEDHKIEEHIPACELR----------------RRDHARNENQAPPRCYTSHGRSDDALLRESSL----------------------LMQSDYDP--NKAPTVVVHNKG------EATAANGYIINGLETNVAAGNGLRGIRWLR 163
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A2D4BRW4_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BRW4_PYTIN)

HSP 1 Score: 54.7 bits (130), Expect = 2.650e-5
Identity = 30/61 (49.18%), Postives = 39/61 (63.93%), Query Frame = 1 
Query:  400 RDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLR 582
            RD L+        ++ P V +H  RG    + D +A+AYIING E N+AAG+GLRGIRWLR
Sbjct:  908 RDSLLLQPSDXDPNKAPTVVLHH-RG----ESDAAASAYIINGLENNIAAGNGLRGIRWLR 963
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: A0A8K1FRW7_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FRW7_PYTOL)

HSP 1 Score: 52.0 bits (123), Expect = 5.730e-5
Identity = 28/61 (45.90%), Postives = 37/61 (60.66%), Query Frame = 1 
Query:  400 RDDLVQDSVHSFEHEGPKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLR 582
            RD L+  +     ++ P V +H    T       +A+AYIING E N+AAG+GLRGIRWLR
Sbjct:  114 RDSLLLQTSDYDPNKAPTVVLHHRGETH-----AAASAYIINGLENNIAAGNGLRGIRWLR 169
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BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Match: W4FSG6_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FSG6_9STRA)

HSP 1 Score: 51.6 bits (122), Expect = 6.150e-5
Identity = 26/45 (57.78%), Postives = 30/45 (66.67%), Query Frame = 1 
Query:  448 PKVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLR 582
            P V +H+        E  +A  YIING ETNVAAG+GLRGIRWLR
Sbjct:  115 PTVVVHNKG------EATAANGYIINGIETNVAAGNGLRGIRWLR 153
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig1387.3072.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
A0A6H5L132_9PHAE1.800e-6658.67Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LHB0_ECTSI6.260e-4647.96Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6S9KAU9_HETAK5.610e-1831.96Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A835Z3A3_9STRA3.300e-1065.22Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A485KQE5_9STRA1.080e-929.63Aste57867_10453 protein n=1 Tax=Aphanomyces stella... [more]
A0A2D4BRW4_PYTIN2.650e-549.18Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A8K1FRW7_PYTOL5.730e-545.90Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
W4FSG6_9STRA6.150e-557.78Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig1387contigH-canaliculatus_F_contig1387:12339..14643 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score156.0
Seed ortholog evalue1.8e-35
Seed eggNOG ortholog2880.D8LHB0
EggNOG free text desc.Phosphatidic acid phosphatase. Source PGD
EggNOG OGs2CZYN@1,2SC5I@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-24_001230.1
Exons4
Model size591
Cds size591
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig1387.3072.1prot_H-canaliculatus_F_contig1387.3072.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig1387 12339..14643 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929128.55893-CDS-H-canaliculatus_F_contig1387:12338..123921622929128.55893-CDS-H-canaliculatus_F_contig1387:12338..12392Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 12339..12392 +
1696845689.1720073-CDS-H-canaliculatus_F_contig1387:12338..123921696845689.1720073-CDS-H-canaliculatus_F_contig1387:12338..12392Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 12339..12392 +
1622929128.5772784-CDS-H-canaliculatus_F_contig1387:12751..128741622929128.5772784-CDS-H-canaliculatus_F_contig1387:12751..12874Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 12752..12874 +
1696845689.1854076-CDS-H-canaliculatus_F_contig1387:12751..128741696845689.1854076-CDS-H-canaliculatus_F_contig1387:12751..12874Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 12752..12874 +
1622929128.5916855-CDS-H-canaliculatus_F_contig1387:13290..134821622929128.5916855-CDS-H-canaliculatus_F_contig1387:13290..13482Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 13291..13482 +
1696845689.1941156-CDS-H-canaliculatus_F_contig1387:13290..134821696845689.1941156-CDS-H-canaliculatus_F_contig1387:13290..13482Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 13291..13482 +
1622929128.6031084-CDS-H-canaliculatus_F_contig1387:14421..146431622929128.6031084-CDS-H-canaliculatus_F_contig1387:14421..14643Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 14422..14643 +
1696845689.2042296-CDS-H-canaliculatus_F_contig1387:14421..146431696845689.2042296-CDS-H-canaliculatus_F_contig1387:14421..14643Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig1387 14422..14643 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig1387.3072.1

>prot_H-canaliculatus_F_contig1387.3072.1 ID=prot_H-canaliculatus_F_contig1387.3072.1|Name=mRNA_H-canaliculatus_F_contig1387.3072.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=197bp
RVGDVVERDIDGIWFPAKVLAVHERRFPTDSSGSCSNVWQPRQLAFDLVY
LDDQNRESEVPEGDLRVVHDYGSRTKDNLLLLLREEDRSTSRAGEESRLG
GGGSIGAGRRKTHTEKDEEEAETETSTHYDDRGRDDLVQDSVHSFEHEGP
KVTIHSSRGTFDTKEDGSATAYIINGPETNVAAGSGLRGIRWLRGP*
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mRNA from alignment at H-canaliculatus_F_contig1387:12339..14643+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig1387.3072.1 ID=mRNA_H-canaliculatus_F_contig1387.3072.1|Name=mRNA_H-canaliculatus_F_contig1387.3072.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=2305bp|location=Sequence derived from alignment at H-canaliculatus_F_contig1387:12339..14643+ (Hapterophycus canaliculatus Oshoro5f female)
CGAGTGGGAGATGTTGTCGAGAGGGATATCGATGGTATCTGGTTCCCGGC
GAAGGTAATTGACAATTAGGTTGCGGCAAGTTCGCAGCTGCAAGTTCGCA
GCTCCCATTGAGCCCGTTGTACAATCTCCGGTTGTATCTTGAACAGACTT
GTAGACAAGAAATGGCCCCCAATCCCTGACCCTTCAATCTCGGGGAACTT
CATTCTTTCCTATGAGACTGCACGCATCTACCCCCAGGGCACTAGAACTG
GTGTTCATGTCATATCAGGTTGGTCTCGAGAAGAAAGCAGGACAATTGCC
AATTAGCCACCTGCACCTCATGATCCTGTCGAAGCTTGAAGATACAGAAG
AGAGTAACAGCTGGTGTTTAGTAATCGCCTATGTTCCATAATTCCCATCG
GCACATCTCCAAGGTACTGGCCGTGCACGAGAGGAGATTTCCGACAGATT
CGTCGGGTAGCTGCTCCAATGTCTGGCAGCCTCGGCAACTGGCATTTGAC
CTCGTCTATTTGGACGACCAGAACCGAGAGAGCGAGGTGTGTTTCAGAAT
GTCCCATTGACAACGCGCGAGGAGATTCACTTTCGACGCTTCAACCATGT
GTGCACACCACCTAGCAAAGCATGAAATGCTGGCTCAAGACGAGTAGACT
TATGGCTCAGCACCATCGAAATCGCTTGAGTAATTCGAGAGCGTTGTGGA
TGGCGCTGAGTCAGCAAGCAGAAATAGAGGTTTGATATACTGTAAATACA
GCGATGGGTGTTCCTCTTCGATTGCTCTATGGGCTCAAACGATATATTTG
TGTTCCACTGAGAATGAACGCCCCTTGGCTGTTGTTTGTTGACATTACGG
TATGTAGCTGATCCGTCTTGGTGCGTTTAGCTTTGCGTGTACGGATTACG
ATGCAAAGAACGCATGTCCACTGCTTGTTCTTCAATCGAACCATGTTGAC
AGGTACCGGAAGGGGACCTCCGCGTCGTCCACGACTACGGTAGTAGAACC
AAAGACAACTTGCTGTTACTCTTGCGCGAGGAAGACCGATCTACGAGTAG
GGCTGGCGAGGAATCACGCTTGGGCGGTGGCGGGAGCATCGGCGCGGGGC
GCCGCAAAACGCACACGGAAAAGGACGAAGAAGAAGCGGAGACCGTGTTT
CGAAGGGAGGTGGGTTGGAGCTCGAACGGAAGGCGAAGAACGGGCCGCGA
CAAATCTTTTTGAACTCAACAGAAGCCTCGGGGTACGCCTCACACGACGT
ACCTCAGGAGGTGTTCTAACGGTGTTGTTATGTTTTCTTAAAAATCTTCA
TATTTGCGTGTGAAACATGGTGGCTTCGATCCAGACACCAAAGACTGGCA
ATTGACAGTCAATAACGTTGCTTGTCGGCACCACAGTTAGTGCTTGTACC
ACAGAGCACGGACACGGAATACTTTCACGGTTTATCGTATTCGTCGTCCA
GTCTGAGGCCGAAGCCCAAACCGCATGACGTCGTACATGCGTGCGCCAGT
GGGCAATTTCAGTATGTGGTCGGAGCAATTCACTTCAAACTGTCCCTCGA
ATGAAACCCTCACTGAATGGAATCGACAAAAGTCCCTTGAGATGGACAGT
GTCTCAGGTTGGTATTCCTTCTGTGTTGTTGACTTGTTTTATAAGCTCAC
ACCAAAACACCAAGAACTGCTTCCAACTTAAATATAACCTGAACAGCGTA
AACTATAGTATATTCAGGTCATCTTCACGCGGTTCGAAACTTACCACGGT
GACCCGTTCTGCGCGGTCATCGTTGGGAAAAGGCATCGATCAGCGCGGAA
AAAAAGACAAAACGAGAAAAATCGCACGACCGGATTGATTGCTTACCTCC
AGCTTAGACTGACAACAAGCCCTGCAATGAGGCAGTGATCAGTTTTACTC
TTTTAAGCTTCTTGGTGACAGACCCCCGACAAACAGCGATGTATAAAATT
GATCCAAAGAATAGCTGCCGTGCGCATGGCTCTGAAGCACTGCTGCTGCT
GCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCCACGTGCTGCTGTTG
TCACTGCGTGCTGTGATTTAGGGCGAGGAGAAGGAAACGAGCACCCACTA
CGACGACAGAGGAAGGGACGACCTCGTCCAGGATTCCGTCCATAGCTTCG
AGCACGAAGGACCGAAGGTCACAATTCATTCCTCACGCGGGACTTTCGAC
ACCAAGGAGGACGGCTCCGCCACGGCGTACATCATCAACGGCCCCGAAAC
CAACGTTGCCGCGGGGAGCGGGCTCAGAGGGATCCGTTGGCTTAGGGGGC
CTTGA
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig1387:12339..14643+

>mRNA_H-canaliculatus_F_contig1387.3072.1 ID=mRNA_H-canaliculatus_F_contig1387.3072.1|Name=mRNA_H-canaliculatus_F_contig1387.3072.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=1182bp|location=Sequence derived from alignment at H-canaliculatus_F_contig1387:12339..14643+ (Hapterophycus canaliculatus Oshoro5f female)
CGAGTGGGAGATGTTGTCGAGAGGGATATCGATGGTATCTGGTTCCCGGC
GAAGCGAGTGGGAGATGTTGTCGAGAGGGATATCGATGGTATCTGGTTCC
CGGCGAAGGTACTGGCCGTGCACGAGAGGAGATTTCCGACAGATTCGTCG
GGTAGCTGCTCCAATGTCTGGCAGCCTCGGCAACTGGCATTTGACCTCGT
CTATTTGGACGACCAGAACCGAGAGAGCGAGGTACTGGCCGTGCACGAGA
GGAGATTTCCGACAGATTCGTCGGGTAGCTGCTCCAATGTCTGGCAGCCT
CGGCAACTGGCATTTGACCTCGTCTATTTGGACGACCAGAACCGAGAGAG
CGAGGTACCGGAAGGGGACCTCCGCGTCGTCCACGACTACGGTAGTAGAA
CCAAAGACAACTTGCTGTTACTCTTGCGCGAGGAAGACCGATCTACGAGT
AGGGCTGGCGAGGAATCACGCTTGGGCGGTGGCGGGAGCATCGGCGCGGG
GCGCCGCAAAACGCACACGGAAAAGGACGAAGAAGAAGCGGAGACCGTAC
CGGAAGGGGACCTCCGCGTCGTCCACGACTACGGTAGTAGAACCAAAGAC
AACTTGCTGTTACTCTTGCGCGAGGAAGACCGATCTACGAGTAGGGCTGG
CGAGGAATCACGCTTGGGCGGTGGCGGGAGCATCGGCGCGGGGCGCCGCA
AAACGCACACGGAAAAGGACGAAGAAGAAGCGGAGACCGAAACGAGCACC
CACTACGACGACAGAGGAAGGGACGACCTCGTCCAGGATTCCGTCCATAG
CTTCGAGCACGAAGGACCGAAGGTCACAATTCATTCCTCACGCGGGACTT
TCGACACCAAGGAGGACGGCTCCGCCACGGCGTACATCATCAACGGCCCC
GAAACCAACGTTGCCGCGGGGAGCGGGCTCAGAGGGATCCGTTGGCTTAG
GGGGCCTTGAGAAACGAGCACCCACTACGACGACAGAGGAAGGGACGACC
TCGTCCAGGATTCCGTCCATAGCTTCGAGCACGAAGGACCGAAGGTCACA
ATTCATTCCTCACGCGGGACTTTCGACACCAAGGAGGACGGCTCCGCCAC
GGCGTACATCATCAACGGCCCCGAAACCAACGTTGCCGCGGGGAGCGGGC
TCAGAGGGATCCGTTGGCTTAGGGGGCCTTGA
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