mRNA_H-paniculata_contig19292.4869.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig19292.4869.1
Unique NamemRNA_H-paniculata_contig19292.4869.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: D7FIV7_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FIV7_ECTSI)

HSP 1 Score: 140 bits (352), Expect = 1.510e-36
Identity = 65/93 (69.89%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:    1 VAEAVARERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALSDTYVDR 279
            VAEA++RERG ++F+HPVIPVLDETR MV  YNRVFRR+V+KSK L WLDFF+ LLD+TG  L P FRLDGTHLA GY+ LLE+AL+D +V+R
Sbjct:  758 VAEALSRERGLRIFVHPVIPVLDETRGMVKTYNRVFRRMVDKSKHLHWLDFFEQLLDDTGSSLRPNFRLDGTHLAAGYVGLLEKALADAWVER 850
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A3F2RPI4_9STRA (Uncharacterized protein n=2 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3F2RPI4_9STRA)

HSP 1 Score: 105 bits (261), Expect = 1.210e-24
Identity = 49/83 (59.04%), Postives = 63/83 (75.90%), Query Frame = 1 
Query:   13 VARERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALS 261
            V R+  F+ +IHPV+PVLDETR MV  YN++F++ V KS++ +WLDFFDDL+ +T  KL PEFRLDGTHL P YL  LE  L+
Sbjct:  341 VVRKYEFEAYIHPVVPVLDETRGMVVQYNKLFQKRVAKSRICKWLDFFDDLVYQTPYKLRPEFRLDGTHLHPSYLTNLEVELN 423
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: UPI000644823E (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644823E)

HSP 1 Score: 102 bits (255), Expect = 1.890e-23
Identity = 44/86 (51.16%), Postives = 63/86 (73.26%), Query Frame = 1 
Query:   28 GFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALSDTYVDRTS 285
             ++ +IHPV+PVLD+TR+MV A+N++F+  V K K L WLDFFD+LLD TG     E+ +DGTH+ P Y+RL+ER ++D Y  + S
Sbjct:  699 NYKAYIHPVVPVLDQTRTMVKAFNKIFKEKVMKVKELVWLDFFDELLDSTGNGFNQEYAMDGTHMNPSYVRLVERTINDHYRSKQS 784
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: W2QE94_PHYPN (Uncharacterized protein n=41 Tax=Phytophthora TaxID=4783 RepID=W2QE94_PHYPN)

HSP 1 Score: 100 bits (250), Expect = 8.980e-23
Identity = 47/76 (61.84%), Postives = 59/76 (77.63%), Query Frame = 1 
Query:   31 FQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERAL 258
            F V+IHPV+PVLDETRS+V +YN++F++ V++S + RWLDF DDL+     KL PEFRLDGTHL P YL  LE AL
Sbjct:  749 FDVYIHPVVPVLDETRSLVISYNKLFQKRVDESSISRWLDFHDDLVCGDPPKLRPEFRLDGTHLHPSYLSKLEEAL 824
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A6A3LA10_9STRA (Uncharacterized protein n=6 Tax=Phytophthora TaxID=4783 RepID=A0A6A3LA10_9STRA)

HSP 1 Score: 99.8 bits (247), Expect = 2.280e-22
Identity = 45/77 (58.44%), Postives = 60/77 (77.92%), Query Frame = 1 
Query:   31 FQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALS 261
            F+ +IHPV+PVLDETR++V  YN++FR+ V KS + +WLDFFDDL+ +   KL P+ RLDGTHL P YL  LE+AL+
Sbjct:  742 FEAYIHPVVPVLDETRALVIQYNQLFRKQVAKSTICKWLDFFDDLVCDDPPKLRPDLRLDGTHLHPAYLVNLEKALN 818
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A2D4BJE6_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BJE6_PYTIN)

HSP 1 Score: 99.0 bits (245), Expect = 4.200e-22
Identity = 44/78 (56.41%), Postives = 58/78 (74.36%), Query Frame = 1 
Query:   28 GFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALS 261
            GF+VFIHP++PV +ETR +V  YN +F+  VNKS   RWLDFFDDL++ +  +L P + LDGTHL P YL L+ER L+
Sbjct:  607 GFEVFIHPIVPVXNETRPLVIQYNGIFQSRVNKSDKCRWLDFFDDLIEGSPARLRPRYALDGTHLHPSYLALVERELN 684
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A6A4G484_9STRA (Uncharacterized protein n=1 Tax=Phytophthora rubi TaxID=129364 RepID=A0A6A4G484_9STRA)

HSP 1 Score: 98.2 bits (243), Expect = 6.980e-22
Identity = 44/77 (57.14%), Postives = 60/77 (77.92%), Query Frame = 1 
Query:   31 FQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALS 261
            F+ +IHPV+PVLDETR++V  YN++FR+ V +S + +WLDFFDDL+ +   KL P+ RLDGTHL P YL  LE+AL+
Sbjct:  459 FEAYIHPVVPVLDETRALVIQYNQLFRKHVAQSTICKWLDFFDDLVCDNPPKLRPDLRLDGTHLHPAYLVNLEKALN 535
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A833TQX5_PHYIN (Uncharacterized protein n=1 Tax=Phytophthora infestans TaxID=4787 RepID=A0A833TQX5_PHYIN)

HSP 1 Score: 96.3 bits (238), Expect = 3.040e-21
Identity = 45/85 (52.94%), Postives = 57/85 (67.06%), Query Frame = 1 
Query:   13 VARERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALSDT 267
            V  +  F V+IHPV+PVLDETR +V  YN++F + V KS + +WLDFFD+L+ E   KL P FRLDGTH+ P YL  L  A   T
Sbjct:  412 VVEKYAFDVYIHPVVPVLDETRLLVIQYNKLFEKRVVKSSICKWLDFFDELVCEDPPKLRPNFRLDGTHMHPSYLSTLAAAFKRT 496
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A7S2D369_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2D369_9STRA)

HSP 1 Score: 90.5 bits (223), Expect = 3.200e-21
Identity = 43/85 (50.59%), Postives = 61/85 (71.76%), Query Frame = 1 
Query:   19 RERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLD-FFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALSDTY 270
            ++RGF++FIHPVIPVL+ETR++V AYN++F+  VN+ + L W+D  F  LL   G KL  E+ LDGTHL P Y+ LL   L++ +
Sbjct:   54 KQRGFRMFIHPVIPVLNETRNIVKAYNKIFKARVNEVEGLEWIDGVFTRLLTPDGSKLKSEYELDGTHLHPSYVSLLGEGLTEIW 138
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BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Match: A0A8K1FF76_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FF76_PYTOL)

HSP 1 Score: 95.5 bits (236), Expect = 6.990e-21
Identity = 46/83 (55.42%), Postives = 58/83 (69.88%), Query Frame = 1 
Query:   13 VARERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLDFFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALS 261
            V +  G +VFIHP++PVL ETR +V  YN++ R  VNKS    WLDFFDDLL+ +  KL   + LDGTHL P YL++LER LS
Sbjct:  755 VVKRFGLEVFIHPIVPVLKETRHLVIQYNQILRDRVNKSNNSTWLDFFDDLLEGSPAKLRSCYELDGTHLHPSYLQVLERELS 837
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig19292.4869.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FIV7_ECTSI1.510e-3669.89Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A3F2RPI4_9STRA1.210e-2459.04Uncharacterized protein n=2 Tax=Phytophthora kerno... [more]
UPI000644823E1.890e-2351.16hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
W2QE94_PHYPN8.980e-2361.84Uncharacterized protein n=41 Tax=Phytophthora TaxI... [more]
A0A6A3LA10_9STRA2.280e-2258.44Uncharacterized protein n=6 Tax=Phytophthora TaxID... [more]
A0A2D4BJE6_PYTIN4.200e-2256.41Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A6A4G484_9STRA6.980e-2257.14Uncharacterized protein n=1 Tax=Phytophthora rubi ... [more]
A0A833TQX5_PHYIN3.040e-2152.94Uncharacterized protein n=1 Tax=Phytophthora infes... [more]
A0A7S2D369_9STRA3.200e-2150.59Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A8K1FF76_PYTOL6.990e-2155.42Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig19292contigH-paniculata_contig19292:1309..4699 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score141.7
Seed ortholog evalue1.7e-31
Seed eggNOG ortholog2880.D7FIV7
Hectar predicted targeting categoryother localisation
EggNOG OGs28R2N@1,2QXRP@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size288
Cds size288
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929471.4262211-CDS-H-paniculata_contig19292:1308..14951622929471.4262211-CDS-H-paniculata_contig19292:1308..1495Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig19292 1309..1495 -
1691480876.073229-CDS-H-paniculata_contig19292:1308..14951691480876.073229-CDS-H-paniculata_contig19292:1308..1495Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig19292 1309..1495 -
1622929471.4402776-CDS-H-paniculata_contig19292:4598..46991622929471.4402776-CDS-H-paniculata_contig19292:4598..4699Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig19292 4599..4699 -
1691480876.0855467-CDS-H-paniculata_contig19292:4598..46991691480876.0855467-CDS-H-paniculata_contig19292:4598..4699Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig19292 4599..4699 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig19292.4869.1prot_H-paniculata_contig19292.4869.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig19292 1309..4699 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig19292.4869.1

>prot_H-paniculata_contig19292.4869.1 ID=prot_H-paniculata_contig19292.4869.1|Name=mRNA_H-paniculata_contig19292.4869.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=96bp
VAEAVARERGFQVFIHPVIPVLDETRSMVTAYNRVFRRVVNKSKVLRWLD
FFDDLLDETGKKLLPEFRLDGTHLAPGYLRLLERALSDTYVDRTS*
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mRNA from alignment at H-paniculata_contig19292:1309..4699-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig19292.4869.1 ID=mRNA_H-paniculata_contig19292.4869.1|Name=mRNA_H-paniculata_contig19292.4869.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=3391bp|location=Sequence derived from alignment at H-paniculata_contig19292:1309..4699- (Halopteris paniculata Hal_grac_a_UBK monoicous)
GTGGCTGAGGCTGTGGCGAGAGAGCGAGGATTCCAGGTGTTTATTCACCC
GGTGATCCCTGTGCTGGATGAAACGCGCTCCATGGTGACGGCGTACAATC
GGTAAGAAATTCCGGTGAACGGTGCTTTTGTCAGAAGCGAAAGTCTGCAG
CATCGATATAGCTATAGCTTTCTAGCTAGCTACACCACAAGCGATATGTG
GGGGCTGTTGTGTTTATTGGCCAACCAAACTGTGCAGAAAACCCAGCGGC
GTTTTCACGTGAATTCGTGAAGATAAGCTTTCGGAATAGAAAAAGACATT
TCCGTAGTTGCAAGTAATAAAAGAGAATGATTCATGACGCGGGCGGGCGG
GCGGGGGGGCGGGGGGAGGAGGGGGGGGGGGGTGGGGATACTCACGTTTT
GCATGCCGTTGTTGTTAGACCGTTGACCATGCGTTCTCTAGTGTACTACA
GCCACGGCGACGCCCTTTTAGTCATATTATCCCGTTGTTTTTTCTTTTGT
GTATCGCTATTTATTTTATGTGAATGTCAGTGAGGGTAGGCTCTAGCCTT
GAGTTCTATTGTGACCCGTGGCCGGATCACCGGTAGCGTGTACCCCAGTA
GCCGGTAGGGCCTGCTGCTCCGTCAGGGTTCATTGCTTTGCATTCATGCT
ATATATGGATGTACTATACACACGCGAGTGGTATTATCGTTCTCCCTGTC
ATCCCTCCGACGTAATCATTTTTAATTTCGTTGATGAATGTATGGCCAGG
CTCTCTAGTGGAGCAGTAAGGCAGTGAGTGACCTTCAGGCTCCCCTTTGC
CGTCTGTAGTCACGTCAGGAGGAGTAGCGCACCTACTATGGGTCAGAGCT
CCTTATGATGGCTGTGATCATCGATGTGTTTGCGCACTACAGCGAGTAAA
TGGCGATACGACACGCGGAAATTTTTCAACGGTCCGTCACATTGTAGTAC
CTCTACCGGTATGCTGCCCATTTACTGGTCTTAATTCAGTGCTTTTGGAC
TTGATGAAACAATGGGTTCGGATATACGGGGTACACGCTATCTTTTCCCT
CCTTTCCTGCTTGCCATATTTTGATCAAGTTCGATATAATGTACTGAATT
ATGACCACTATATGGGGCTGTAACGGTGTAAGGATTTTTTTTTTCCCATT
TTTTTTTTTGTGTTGAAGCATTACTGAAGGAGAAAGTACGACTGAAGCAC
TCATCCGGTTGAAAAATACTGATTTGGAGATGATGATAATGTCAGATTGC
CCAACATACAAGCATTTGTCTATTTTGGTGCAATAGGCAATAGATAGCTA
ATCATATCTGCAAACGTTATGACAACAGTGGAAATAGACACTTGTGTCGA
GAGTGGGCATTCGTTGCTACAATTTCCCCCGCGATTAATTCAATTATTTG
AAATCGCGCGGCGAAGAAGAAAAATTCGGCCCAATAAATAGCATGGGTGC
CTAGTTCTTCCATGTGTACCAATACATTTTAGGGTGCTCACGTACTGTAG
CAGAATTATCAGTACTCGTTTTTTTTTTGTTTTTTTTTCTTGACTACCCT
GAGTGACGATGGGATGGGATACAACGGTGCGAACTCCAGCCTCTGAATTG
ACCGGATTATGGGTATATCACCTATTGTTTATTTTTTTCATCGGATTCGG
CGCCAGGGGTTGTTTCGGAATGAATCCGAAGCCCTAGCAAAAAAGGTGGA
ATTAGTTGGCTGAAGTAACTCATTATGACCCCCCTAACCATAAAGAGTGA
AATATACCATGGAACATGGGATACAGTATTGACCCGTATCTATTCGTTTT
TAGTTTTATTGTTCAACAGGAGTAATCGTGAAAGATCCCTATTTATCCTG
AACTTTCAGGCGAATATTTAGAACATTTTCGTCGCTAATGGTCATGCTCC
GATCGCTTGTATTCGGCCGGGGGAGAGAGGGGGGGGGGGGTGTCGTATAT
TCGTATATTATTGCACTTTCAGGTGACCGCAATTCTCAAAGCCGCGTGCT
TCCACGGTGTAGGTGCTGTTTCTCCTGGTGCTGAAGTTCTCCCCGGCACC
CGGCGCTCAATGAATAATACATTGGTGTCGCTGTGTTCTATGGTACAGGA
AAATTTAGAAAAATTCATTCGCAAATATGTGAAAAGAGTGTTCTGTCCTC
TACGGACTCTATAATAGTAGGAATTATCCCTGTTTTTTCAACCCCCGGAA
GGCTGAGGCAAAAACCTCTATCCGATGCTTCCATACTTGAGTATTTTACC
GAAGTATCTCTACATCTCCTTTAACAGAACAGTTATATATTCCTCTGCGG
GGGTAGGCGCCACCCCGGTTCGGCTGAACCAGGTAGGCAGTGTGGGGGTT
GCATCCACTAAAAGTCCTCTAACTAGACTTCCAGTCATGCTCAGTTCACG
AGCTACCCTACCCGTTCAGGCGTAGGTCCGTGGATTACTATTAGCCCTTT
CTACAAGGTACTCTCCACATCACCCCTCCTTTAATCAGGCTTTTTGCTTT
TTTTACAAGTGCCTACTAGGGGTAACCGGTTTCGCCGACTCGCGGTGAGA
ACGTTGACTTCTTTCTTGTTTACAATCTTCCCTCCTCTTGCGATTGTATT
ATTACTTGAAGTTTGTATGGAATTTTTGTCCATGTTCCCCCACCATTTTT
ACGGCCTGCTTAACTCGATTTTTGTTTTTATTCCTTGGATGATTATGGTC
GAGTAAATCCTGTAATCGCGACCTTCAAATCCTGTGTTGAGGGTTCCTAC
CGACGACACCATCACCATCATGAAAAGGGTGTCCTGTCCTCTATGGGCTC
TAGTGGGAATTTTGTCTCTTTCTATATAGGGTGCTTATCACATATACTGG
TTTATTTATTTGATATTTTTGTAATCAACCAACGCTTGTTTCTTGGTTAA
CGGGCGTCTTTCTTTGAGTGAACCTTCGTTAAAATCCATCATGAGCTAAA
TAACTTCGAAGAATATATATATATATATATATTAGTTAAACTAATGTTTT
TTGTAACGATGCTCTACTGCAATAACAACATTTTTCACATGCATTTTGTG
TTGTTTTTAATCATGAACTATTATTGTTTAATGCTCCCCCCCCCCCCCTG
TAACCATTAACTGTAGCTAGTGTTACTGCCCTACGAAAAACGTTCTACTA
AATACTTCTCTACTCTATATTCCCCGCCCGCGTACCCCTCCCGTCGCTTT
CAAGCGTGTTTCGGCGCGTCGTTAACAAGTCTAAAGTGCTCAGGTGGCTG
GATTTCTTCGACGACTTGTTAGACGAGACGGGAAAAAAGCTGCTCCCCGA
ATTCCGCTTGGACGGCACCCATCTGGCGCCGGGTTACCTGCGTCTGCTAG
AGAGAGCGCTGAGCGACACTTATGTCGACCGCACTTCGTGA
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Coding sequence (CDS) from alignment at H-paniculata_contig19292:1309..4699-

>mRNA_H-paniculata_contig19292.4869.1 ID=mRNA_H-paniculata_contig19292.4869.1|Name=mRNA_H-paniculata_contig19292.4869.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=576bp|location=Sequence derived from alignment at H-paniculata_contig19292:1309..4699- (Halopteris paniculata Hal_grac_a_UBK monoicous)
GTGGCTGAGGCTGTGGCGAGAGAGCGAGGATTCCAGGTGTTTATTCACCC
GGTGATCCCTGTGCTGGATGAAACGCGCTCCATGGTGACGGCGTACAATC
GGTGGCTGAGGCTGTGGCGAGAGAGCGAGGATTCCAGGTGTTTATTCACC
CGGTGATCCCTGTGCTGGATGAAACGCGCTCCATGGTGACGGCGTACAAT
CGCGTGTTTCGGCGCGTCGTTAACAAGTCTAAAGTGCTCAGGTGGCTGGA
TTTCTTCGACGACTTGTTAGACGAGACGGGAAAAAAGCTGCTCCCCGAAT
TCCGCTTGGACGGCACCCATCTGGCGCCGGGTTACCTGCGTCTGCTAGAG
AGAGCGCTGAGCGACACTTATGTCGACCGCACTTCGTGACGTGTTTCGGC
GCGTCGTTAACAAGTCTAAAGTGCTCAGGTGGCTGGATTTCTTCGACGAC
TTGTTAGACGAGACGGGAAAAAAGCTGCTCCCCGAATTCCGCTTGGACGG
CACCCATCTGGCGCCGGGTTACCTGCGTCTGCTAGAGAGAGCGCTGAGCG
ACACTTATGTCGACCGCACTTCGTGA
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