mRNA_H-paniculata_contig13802.2335.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig13802.2335.1
Unique NamemRNA_H-paniculata_contig13802.2335.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: D8LL63_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LL63_ECTSI)

HSP 1 Score: 209 bits (533), Expect = 1.680e-63
Identity = 105/132 (79.55%), Postives = 117/132 (88.64%), Query Frame = 1
Query:    4 ARFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP-RDYG 396
            +RFVH+SSAGVTRPGRPDLD++ EPPAVRMN+MLS+LLTYKLKGEDVIR+SGIP TIIRPCALTEEP GAP+IV QGD +KGKISRDDIAELAV SLLTP+A GLTFEVKSDLAFST+W+      P R YG
Sbjct:  309 SRFVHLSSAGVTRPGRPDLDMDTEPPAVRMNEMLSHLLTYKLKGEDVIRDSGIPATIIRPCALTEEPAGAPMIVGQGDYLKGKISRDDIAELAVSSLLTPEASGLTFEVKSDLAFSTLWQGAPQGAPARSYG 440          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A6H5KMW5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KMW5_9PHAE)

HSP 1 Score: 211 bits (537), Expect = 1.800e-62
Identity = 106/132 (80.30%), Postives = 116/132 (87.88%), Query Frame = 1
Query:    4 ARFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP-RDYG 396
            +R VH+SSAGVTRPGRPDLD++ EPPAVRMN+MLSYLLTYKLKGEDVIR+SGIP TIIRPCALTEEP GAP+IV QGD +KGKISRDDIAELAV SLLTP+A GLTFEVKSDLAFST+WE      P R YG
Sbjct:  492 SRLVHLSSAGVTRPGRPDLDIDTEPPAVRMNEMLSYLLTYKLKGEDVIRDSGIPATIIRPCALTEEPAGAPMIVGQGDYLKGKISRDDIAELAVSSLLTPEASGLTFEVKSDLAFSTLWEGAPQGTPARSYG 623          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: W7TT61_9STRA (Nadh:ubiquinone oxidoreductase complex i intermediate-associated protein 30 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TT61_9STRA)

HSP 1 Score: 171 bits (432), Expect = 1.100e-47
Identity = 86/125 (68.80%), Postives = 98/125 (78.40%), Query Frame = 1
Query:    7 RFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRR 381
            RFVH+SSAGV RPGRP + +E EPPAVRMNDML  +LTYKLKGE+ IR SG+P TIIRPCALTEEP   P+ V  GD IKGK+SRDD+A LAV++L  P+A  LTFEVKS LAFS  W AED  R
Sbjct:  455 RFVHISSAGVERPGRPGVVLEEEPPAVRMNDMLGGILTYKLKGEEAIRASGLPYTIIRPCALTEEPANMPLEVDVGDTIKGKVSRDDVARLAVYALACPEATDLTFEVKSSLAFSQQWTAEDAAR 579          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A0G4FWF9_VITBC (Uncharacterized protein n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4FWF9_VITBC)

HSP 1 Score: 169 bits (428), Expect = 1.200e-47
Identity = 81/123 (65.85%), Postives = 98/123 (79.67%), Query Frame = 1
Query:    7 RFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDD 375
            R + VSSAGVTRPGRP LD+E EPPAVRMNDML  +LTYKLKGEDV+R+SG+  ++IRPCALTEEP GAP+ + QGD IKGKISR+D+A+L V SLLTP A   T+E+K  L FS  W+ + D
Sbjct:  359 RLIQVSSAGVTRPGRPGLDLEKEPPAVRMNDMLGGILTYKLKGEDVVRDSGVTYSVIRPCALTEEPAGAPLQIGQGDEIKGKISREDVADLVVASLLTPAAANKTYEIKCTLPFSEPWQPKAD 481          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: K8YQM7_NANGC (Nadh:ubiquinone oxidoreductase complex i intermediate-associated protein 30 n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8YQM7_NANGC)

HSP 1 Score: 171 bits (432), Expect = 1.090e-46
Identity = 86/125 (68.80%), Postives = 98/125 (78.40%), Query Frame = 1
Query:    7 RFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRR 381
            RFVH+SSAGV RPGRP + +E EPPAVRMNDML  +LTYKLKGE+ IR SG+P TIIRPCALTEEP   P+ V  GD IKGK+SRDD+A LAV++L  P+A  LTFEVKS LAFS  W AED  R
Sbjct:  455 RFVHISSAGVERPGRPGVVLEEEPPAVRMNDMLGGILTYKLKGEEAIRASGLPYTIIRPCALTEEPANMPLEVDVGDTIKGKVSRDDVARLAVYALACPEATDLTFEVKSSLAFSQQWTAEDAAR 579          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A022QLG4_ERYGU (Uncharacterized protein (Fragment) n=1 Tax=Erythranthe guttata TaxID=4155 RepID=A0A022QLG4_ERYGU)

HSP 1 Score: 157 bits (398), Expect = 1.250e-45
Identity = 80/131 (61.07%), Postives = 98/131 (74.81%), Query Frame = 1
Query:    7 RFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP--RDY 393
            RFVHV SAGVTRP RP LD+  +PPAVR+N  L ++LTYKLKGED+IR SGIP TIIRPCALTEEP GA +IV QGDNI GKISRD++A + + +L +P A   TFEVKS + FS  +  + +  P  +DY
Sbjct:  103 RFVHVGSAGVTRPDRPGLDLSKQPPAVRLNKELDFILTYKLKGEDLIRESGIPYTIIRPCALTEEPAGADLIVEQGDNITGKISRDEVARMCIAALESPYACDKTFEVKSVIPFSEPYTVDPENPPPEKDY 233          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A2P6Q4X2_ROSCH (Putative NAD(P)-binding domain-containing protein n=4 Tax=Rosoideae TaxID=171638 RepID=A0A2P6Q4X2_ROSCH)

HSP 1 Score: 155 bits (392), Expect = 1.480e-45
Identity = 78/131 (59.54%), Postives = 99/131 (75.57%), Query Frame = 1
Query:    7 RFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP--RDY 393
            RFVHV SAGVTRP RP LD+  +PPAVR+N  L ++LT+KLKGED+IR SGIP TI+RPCALTEEP GA +I  QGDNI GKISR+++A++ V +L +P A G TFEVKS + FS  +  + +  P  +DY
Sbjct:   38 RFVHVGSAGVTRPERPGLDLSKQPPAVRLNKELDFILTFKLKGEDLIRESGIPYTIVRPCALTEEPAGADLIFDQGDNITGKISREEVAQICVAALESPYATGKTFEVKSVIPFSEPFTVDPENPPPEKDY 168          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A1R3L5G1_ASPOF (NAD(P)-bd_dom domain-containing protein n=1 Tax=Asparagus officinalis TaxID=4686 RepID=A0A1R3L5G1_ASPOF)

HSP 1 Score: 156 bits (394), Expect = 1.940e-45
Identity = 78/133 (58.65%), Postives = 99/133 (74.44%), Query Frame = 1
Query:    1 MARFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP--RDY 393
            + RFVHVSSAGVTRPGRP LD+  +PPAVR+N  L ++LTYKLKGED+IR SGIP TI+RPCAL+EEP GA +I  QGDNI GKISR+++A + + +L +P A   TFEVKS + FS  +  +    P  +DY
Sbjct:   69 LLRFVHVSSAGVTRPGRPGLDLSKQPPAVRLNKELGFILTYKLKGEDLIRESGIPYTIVRPCALSEEPAGADLIFDQGDNITGKISREEVARICIAALGSPYACNKTFEVKSTIPFSEPFTVDPSNPPPEKDY 201          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: UPI00098E195D (uncharacterized protein At2g34460, chloroplastic-like n=1 Tax=Asparagus officinalis TaxID=4686 RepID=UPI00098E195D)

HSP 1 Score: 154 bits (388), Expect = 1.940e-45
Identity = 77/130 (59.23%), Postives = 97/130 (74.62%), Query Frame = 1
Query:   10 FVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP--RDY 393
            FVHVSSAGVTRPGRP LD+  +PPAVR+N  L ++LTYKLKGED+IR SGIP TI+RPCAL+EEP GA +I  QGDNI GKISR+++A + + +L +P A   TFEVKS + FS  +  +    P  +DY
Sbjct:    2 FVHVSSAGVTRPGRPGLDLSKQPPAVRLNKELGFILTYKLKGEDLIRESGIPYTIVRPCALSEEPAGADLIFDQGDNITGKISREEVARICIAALGSPYACNKTFEVKSTIPFSEPFTVDPSNPPPEKDY 131          
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Match: A0A7S3U8L1_9CHLO (Hypothetical protein n=1 Tax=Picocystis salinarum TaxID=88271 RepID=A0A7S3U8L1_9CHLO)

HSP 1 Score: 164 bits (416), Expect = 3.590e-45
Identity = 80/133 (60.15%), Postives = 102/133 (76.69%), Query Frame = 1
Query:    4 ARFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIRNSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLTPKAGGLTFEVKSDLAFSTVWEAEDDRRP--RDYG 396
            A +VHVSSAGVTRP RP +DV+AEPPAV++ND L  +L+YKLKGED++R +G+P  I+RPCALTEEP GA ++V QGD IKGKISR+D+AEL V  L  P+A   TFE+KS + FST WE +    P  R++G
Sbjct:  477 ATWVHVSSAGVTRPNRPGIDVDAEPPAVKLNDALGGILSYKLKGEDMVREAGMPFAIVRPCALTEEPAGAELLVDQGDTIKGKISREDVAELCVALLSQPEASSTTFEIKSTVPFSTPWEVDPQNPPPPREWG 609          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig13802.2335.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LL63_ECTSI1.680e-6379.55Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KMW5_9PHAE1.800e-6280.30Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
W7TT61_9STRA1.100e-4768.80Nadh:ubiquinone oxidoreductase complex i intermedi... [more]
A0A0G4FWF9_VITBC1.200e-4765.85Uncharacterized protein n=2 Tax=Vitrella brassicaf... [more]
K8YQM7_NANGC1.090e-4668.80Nadh:ubiquinone oxidoreductase complex i intermedi... [more]
A0A022QLG4_ERYGU1.250e-4561.07Uncharacterized protein (Fragment) n=1 Tax=Erythra... [more]
A0A2P6Q4X2_ROSCH1.480e-4559.54Putative NAD(P)-binding domain-containing protein ... [more]
A0A1R3L5G1_ASPOF1.940e-4558.65NAD(P)-bd_dom domain-containing protein n=1 Tax=As... [more]
UPI00098E195D1.940e-4559.23uncharacterized protein At2g34460, chloroplastic-l... [more]
A0A7S3U8L1_9CHLO3.590e-4560.15Hypothetical protein n=1 Tax=Picocystis salinarum ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig13802contigH-paniculata_contig13802:1948..3225 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score212.6
Seed ortholog evalue1.1e-52
Seed eggNOG ortholog2880.D8LL63
Hectar predicted targeting categoryother localisation
EggNOG free text desc.divinyl chlorophyllide a 8-vinyl-reductase activity
EggNOG OGsCOG0702@1,KOG1203@2759
Ec32 ortholog descriptionNADH:ubiquinone oxidoreductase intermediate-associated protein 30
Ec32 orthologEc-25_000150.1
COG Functional cat.GM
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons3
Model size399
Cds size399
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig13802.2335.1prot_H-paniculata_contig13802.2335.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig13802 1948..3225 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929097.5663176-CDS-H-paniculata_contig13802:1947..20311622929097.5663176-CDS-H-paniculata_contig13802:1947..2031Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 1948..2031 +
1691480730.3087137-CDS-H-paniculata_contig13802:1947..20311691480730.3087137-CDS-H-paniculata_contig13802:1947..2031Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 1948..2031 +
1622929097.582242-CDS-H-paniculata_contig13802:2220..23961622929097.582242-CDS-H-paniculata_contig13802:2220..2396Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 2221..2396 +
1691480730.3181689-CDS-H-paniculata_contig13802:2220..23961691480730.3181689-CDS-H-paniculata_contig13802:2220..2396Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 2221..2396 +
1622929097.5968766-CDS-H-paniculata_contig13802:3086..32251622929097.5968766-CDS-H-paniculata_contig13802:3086..3225Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 3087..3225 +
1691480730.3256125-CDS-H-paniculata_contig13802:3086..32251691480730.3256125-CDS-H-paniculata_contig13802:3086..3225Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13802 3087..3225 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig13802.2335.1

>prot_H-paniculata_contig13802.2335.1 ID=prot_H-paniculata_contig13802.2335.1|Name=mRNA_H-paniculata_contig13802.2335.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=133bp
MARFVHVSSAGVTRPGRPDLDVEAEPPAVRMNDMLSYLLTYKLKGEDVIR
NSGIPETIIRPCALTEEPPGAPIIVAQGDNIKGKISRDDIAELAVHSLLT
PKAGGLTFEVKSDLAFSTVWEAEDDRRPRDYG*
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mRNA from alignment at H-paniculata_contig13802:1948..3225+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig13802.2335.1 ID=mRNA_H-paniculata_contig13802.2335.1|Name=mRNA_H-paniculata_contig13802.2335.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1278bp|location=Sequence derived from alignment at H-paniculata_contig13802:1948..3225+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGCGCGTTTCGTGCACGTGAGCTCGGCAGGCGTTACGAGGCCGGGGAG GCCGGATCTCGACGTGGAGGCAGAGCCCCCAGCGGTAAGACCAAAGTTTG TCTCTTCGAGGAGGCAAAAACGGCGAAATCCGAGCACTCAATTCAGCATA ATAGTCAGGTTAAAATGCATAGTACCTGCCCCAATGGCGCAGATGCTGTT CACGTTCGATTTTTTAACATTTCGTCGAGCATCATTACCTGAGCCGTGCC CAATTCCGTCTCATTACTGCAAGGTGCGCATGAACGACATGCTGTCGTAC CTTTTGACATACAAGCTGAAAGGGGAAGACGTGATTCGCAACAGCGGCAT TCCTGAGACAATTATTCGCCCTTGTGCGCTCACGGAAGAACCCCCGGGGG CGCCGATCATAGTGGCTCAGGGGGATAACATCAAAGGCAAGATTAGCAGG TGAGCAAACGAGGGAGCACGCTCGCCAGGGTAAGAGCTCGAGAAATTCGG CTTGAATTTCATCGCCCCAATTTTTTTTTTTTTGTTTTCATGTCGGATGT GACATTGGTGACATTCGTGGTTCAACCTGCCGTTGCTGGTAAAAATAAGA TGAAAGTCTACTGAGCCTATTGGCTTAACAACAATCAGGGTTGCGTGCAC GGTCGCGAGCAGTCCTCAACGTGGGCAGGTGTCTCCATCCGTCAAGGGTA TCCTTGAGGTGGGAGCGACCGATGATGATTTCCCTCATACCTCAGTGAAT AATGGCTCACTCGACTCCGACCAAACCGCTGAAGGGTCGCTGGTCAGTAC GCGGCCACATCTTAGCGGGCAGTTGTCTAGCAACCCGTTCTGGTAATGGA TAATGCCGGCGGTGCCAAGAATTGAGCGTTCGTCCGCAGAGGGAGCGGTT CCCTTGAGTTAACATCTTCTTGCCGGCTTAGGAGGATCTGGTACCAGTGT ACGAGGCCGCCTGGTGTCGAGCTAGCTACCTAAGGTCGAAGAAAGAAATC GTTCTAGGAGAAAATGTTCCTGTATACAAAACAAACTGTCGTGCTGACTT CCATTCATCTCTCGTTCACCACGTCTGGTTGATGCGGTTCTTCGTGTTCG TAACGTGTGTGTGTGTGTGTTTTTTTTGTTTTTTTTCAGGGACGATATCG CGGAGTTGGCAGTCCACTCGCTTTTGACTCCCAAGGCTGGGGGGCTGACG TTTGAGGTCAAGTCTGACCTGGCGTTTAGCACAGTGTGGGAAGCAGAGGA TGATCGGCGACCACGCGATTACGGGTGA
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Coding sequence (CDS) from alignment at H-paniculata_contig13802:1948..3225+

>mRNA_H-paniculata_contig13802.2335.1 ID=mRNA_H-paniculata_contig13802.2335.1|Name=mRNA_H-paniculata_contig13802.2335.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=798bp|location=Sequence derived from alignment at H-paniculata_contig13802:1948..3225+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGCGCGTTTCGTGCACGTGAGCTCGGCAGGCGTTACGAGGCCGGGGAG
GCCGGATCTCGACGTGGAGGCAGAGCCCCCAGCGATGGCGCGTTTCGTGC
ACGTGAGCTCGGCAGGCGTTACGAGGCCGGGGAGGCCGGATCTCGACGTG
GAGGCAGAGCCCCCAGCGGTGCGCATGAACGACATGCTGTCGTACCTTTT
GACATACAAGCTGAAAGGGGAAGACGTGATTCGCAACAGCGGCATTCCTG
AGACAATTATTCGCCCTTGTGCGCTCACGGAAGAACCCCCGGGGGCGCCG
ATCATAGTGGCTCAGGGGGATAACATCAAAGGCAAGATTAGCAGGTGCGC
ATGAACGACATGCTGTCGTACCTTTTGACATACAAGCTGAAAGGGGAAGA
CGTGATTCGCAACAGCGGCATTCCTGAGACAATTATTCGCCCTTGTGCGC
TCACGGAAGAACCCCCGGGGGCGCCGATCATAGTGGCTCAGGGGGATAAC
ATCAAAGGCAAGATTAGCAGGGACGATATCGCGGAGTTGGCAGTCCACTC
GCTTTTGACTCCCAAGGCTGGGGGGCTGACGTTTGAGGTCAAGTCTGACC
TGGCGTTTAGCACAGTGTGGGAAGCAGAGGATGATCGGCGACCACGCGAT
TACGGGTGAGGACGATATCGCGGAGTTGGCAGTCCACTCGCTTTTGACTC
CCAAGGCTGGGGGGCTGACGTTTGAGGTCAAGTCTGACCTGGCGTTTAGC
ACAGTGTGGGAAGCAGAGGATGATCGGCGACCACGCGATTACGGGTGA
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