mRNA_H-paniculata_contig12412.1518.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig12412.1518.1
Unique NamemRNA_H-paniculata_contig12412.1518.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5JAU4_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAU4_9PHAE)

HSP 1 Score: 118 bits (296), Expect = 3.210e-27
Identity = 74/169 (43.79%), Postives = 93/169 (55.03%), Query Frame = 2
Query:  131 GKMSALRQDAVEVAVRTTAASTVLSQLND-TLACAYLVL*QSARDDSDAEDEGHGTHPKKQRRVYVRSPYSESAWARMLQSLD------LTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASLQILERGNSFDDIGQLSLMS 616
            G MS+    AV V    + A    +Q    TL    LVL      D D+E+E       K+RRVY R  Y+ S WA  LQ L       + DP SR ARRFR  F +PYPF VEL+ +V++  WFP   G  D +GRR +P++LKVLA LQIL RGN F DI  +S +S
Sbjct:   16 GTMSSSETGAVNVPAAFSVALAAFNQQQQRTLVAWDLVLDDLDELDPDSEEEEEDNREIKRRRVYPRKDYNISGWAIQLQELKDLEVDGIVDPTSRPARRFRESFRLPYPFFVELVALVKQRDWFPT--GKQDASGRRGIPVQLKVLACLQILARGNCFADIYHMSFIS 182          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5JPH7_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPH7_9PHAE)

HSP 1 Score: 112 bits (280), Expect = 6.060e-25
Identity = 72/158 (45.57%), Postives = 97/158 (61.39%), Query Frame = 2
Query:  161 VEVAVRTTAASTV-LSQLNDTLACAYLVL*QSARDDS--DAEDEGHGTHPKKQRRVYVRSPYSESAWARMLQSL---DLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASLQILERGNSFDDIGQLSLMS 616
            V  A  TTA +T  L QL  ++A  + V+ +   DD   D ED+   +   K+RRVYVR  Y++S W + L  L     TD  +R+ARRFR+ F +PYPF V L+++V++  WFP   G  DV GR+ VP+ELKVLA LQIL RGN F DI  +S +S
Sbjct:   45 VPAAFATTAVATASLQQLQRSVA-VWDVIMEDFEDDLELDTEDD-EASREVKRRRVYVRKDYTKSGWWQELMELRETGETDHTTREARRFRNNFRVPYPFFVHLVELVRDRDWFPT--GESDVTGRQAVPVELKVLACLQILSRGNVFADIYHMSFIS 198          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5KSI7_9PHAE (DDE Tnp4 domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSI7_9PHAE)

HSP 1 Score: 93.2 bits (230), Expect = 1.790e-18
Identity = 53/122 (43.44%), Postives = 70/122 (57.38%), Query Frame = 2
Query:  260 DDSDAEDEGHGTHPKKQRRVYVRSPYSESAWARMLQSLDLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASLQILERGNSFDDIGQLSLMSERV 625
            +DS+ E+E  G   K+ RRV  R  +  SAW   LQ +DL D  +  A+ FR  F +PYPF  EL+K+ +  +WF    G  D A R  +PLELKVL       +GN FDD+ QLS MSE +
Sbjct:   46 EDSEDEEEVVGPKVKRTRRVTQRRDWRTSAWWEKLQDVDLADHTTAAAKVFRERFRVPYPFFQELVKLSRAKRWFSP--GEKDAANREGIPLELKVLG------KGNCFDDVEQLSKMSESI 159          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5KYT9_9PHAE (Uncharacterized protein n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYT9_9PHAE)

HSP 1 Score: 88.6 bits (218), Expect = 3.670e-18
Identity = 58/135 (42.96%), Postives = 81/135 (60.00%), Query Frame = 2
Query:  161 VEVAVRTTAASTV-LSQLNDTLACAYLVL*QSARDDS--DAEDEGHGTHPKKQRRVYVRSPYSESAWARMLQSL---DLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKV 547
            V  A  TTA +T  L QL  ++A  + V+ +   DD   D ED+   +   K+RRVYVR  Y++S W + L  L     TD  +R+ARRFR+ F +PYPF V L+++V++  WFP   G  DV GR+ VP+ELKV
Sbjct:   13 VPAAFATTAVATASLQQLQRSVA-VWDVIMEDFEDDLELDTEDD-EASREVKRRRVYVRKDYTKSGWWQELMELRETGETDHTTREARRFRNNFRVPYPFFVHLVELVRDRDWFPT--GESDVTGRQAVPVELKV 143          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5JP98_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JP98_9PHAE)

HSP 1 Score: 86.3 bits (212), Expect = 6.300e-17
Identity = 56/147 (38.10%), Postives = 86/147 (58.50%), Query Frame = 2
Query:  197 VLSQLNDTLACAYLVL*QSARDDSDAEDEGHGTHPKKQRRVYVRSPYSESAW--ARMLQSLDLT-DPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGR---RMVPLELKVLASLQILERGNSFDDIGQLSLMSE 619
            VLS     L     +L  S R     E++G    P+ +RR+ VR P ++ A   AR+L+  DL  DP++R+A++FR  F +PYPF + L+  V+ G+W        ++ GR   R +P+E+KVLA L+IL RGN FDD+ +++ M E
Sbjct:   13 VLSLAFAALHYQQQLLVASLRQMRREEEDGCDGEPRPKRRLTVR-PRADYALPRARVLEKADLLQDPSTREAKQFRRRFRVPYPFFLSLVDEVKAGKWPVFTTDEFELGGRGTRRCIPVEIKVLAVLRILGRGNYFDDVSEVTGMGE 158          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5K671_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K671_9PHAE)

HSP 1 Score: 89.4 bits (220), Expect = 1.090e-16
Identity = 64/170 (37.65%), Postives = 92/170 (54.12%), Query Frame = 2
Query:  161 VEVAVRTTAASTV-LSQLNDTLACAYLVL*QSARDDS--DAEDEGHGTHPKKQRRVYVRSPYSESAWARMLQSL---DLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASLQILERGNSFDDIGQLSLM----SERVDCPKC 640
            V  A  TTA +T  L QL  ++A  + V+ +   DD   D ED+   +   K+RRVYVR  Y++S W + L  L     TD  +R+ARRFR+ F +PYPF V L+++V++  WFP   G  DV GR+ VP+ELK+  +     RG   + +  +S        R  CP C
Sbjct:  262 VPAAFATTAVATASLQQLQRSVA-VWDVIMEDFEDDLELDTEDD-EASREVKRRRVYVRKDYTKSGWWQELMELRETGETDHTTREARRFRNNFRVPYPFFVHLVELVRDRDWFPT--GESDVTGRQAVPVELKIERT-----RGGVKEIVSGISFPPPAPESRRSCPHC 422          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A6H5JHC3_9PHAE (DDE Tnp4 domain-containing protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHC3_9PHAE)

HSP 1 Score: 83.6 bits (205), Expect = 7.370e-15
Identity = 49/112 (43.75%), Postives = 65/112 (58.04%), Query Frame = 2
Query:  302 KKQRRVYVRSPYSESAWARMLQSLD--LTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRM---VPLELKVLASLQILERGNSFDDIGQLSLMSER 622
            K++R VY R  Y  SAWA ML+  +  L DP ++DAR FR  F +PYP  +EL+K ++   W        D+ GR     +P+ELKVL       RGN FDDI QLS +SE+
Sbjct:   53 KRKRTVYPRPDYHNSAWAIMLREKEDALADPTTKDARLFRRRFRMPYPIFLELVKEIKSAGWKGFTTDATDLGGRGTRPCIPVELKVLG------RGNFFDDIPQLSFISEQ 158          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A835ZAS0_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZAS0_9STRA)

HSP 1 Score: 76.3 bits (186), Expect = 5.210e-14
Identity = 41/87 (47.13%), Postives = 54/87 (62.07%), Query Frame = 2
Query:  302 KKQRRVYVRSPYSESAWARMLQS-LDLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASL 559
            K+ RRVY R  Y  +AW  ML +   L DP SR+A++FR  F +PYP   EL+ +V+E  W P  +   DVAGR  +PLELKV  +L
Sbjct:   36 KRCRRVYERPDYRRTAWMTMLDNEAQLLDPTSREAKKFRRRFRLPYPMFKELVALVREKGWLPCADA--DVAGRPCIPLELKVHCTL 120          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A835ZBP5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBP5_9STRA)

HSP 1 Score: 74.7 bits (182), Expect = 1.590e-12
Identity = 40/84 (47.62%), Postives = 52/84 (61.90%), Query Frame = 2
Query:  302 KKQRRVYVRSPYSESAWARMLQS-LDLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVL 550
            K+ RRVY R  Y  +AW  ML +   L DP SR+A++FR  F +PYP   EL+ +V+E  W P  +   DVAGR  +PLELK L
Sbjct:  100 KRCRRVYERPDYRRTAWMTMLDNEAQLLDPTSREAKKFRRRFRLPYPMFKELVALVREKGWLPCADA--DVAGRPCIPLELKPL 181          
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Match: A0A835YZ80_9STRA (Uncharacterized protein n=2 Tax=Tribonema minus TaxID=303371 RepID=A0A835YZ80_9STRA)

HSP 1 Score: 76.3 bits (186), Expect = 3.290e-12
Identity = 41/87 (47.13%), Postives = 54/87 (62.07%), Query Frame = 2
Query:  302 KKQRRVYVRSPYSESAWARMLQS-LDLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVDVAGRRMVPLELKVLASL 559
            K+ RRVY R  Y  +AW  ML +   L DP SR+A++FR  F +PYP   EL+ +V+E  W P  +   DVAGR  +PLELKV  +L
Sbjct:  767 KRCRRVYERPDYRRTAWMTMLDNEAQLLDPTSREAKKFRRRFRLPYPMFKELVALVREKGWLPCADA--DVAGRPCIPLELKVHCTL 851          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig12412.1518.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
A0A6H5JAU4_9PHAE3.210e-2743.79Uncharacterized protein n=2 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JPH7_9PHAE6.060e-2545.57Uncharacterized protein n=2 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KSI7_9PHAE1.790e-1843.44DDE Tnp4 domain-containing protein (Fragment) n=1 ... [more]
A0A6H5KYT9_9PHAE3.670e-1842.96Uncharacterized protein n=3 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JP98_9PHAE6.300e-1738.10Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K671_9PHAE1.090e-1637.65Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JHC3_9PHAE7.370e-1543.75DDE Tnp4 domain-containing protein n=2 Tax=Ectocar... [more]
A0A835ZAS0_9STRA5.210e-1447.13Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835ZBP5_9STRA1.590e-1247.62Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835YZ80_9STRA3.290e-1247.13Uncharacterized protein n=2 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig12412contigH-paniculata_contig12412:3973..5707 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons2
Model size735
Cds size312
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig12412.1518.1prot_H-paniculata_contig12412.1518.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig12412 4038..5349 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928961.163378-UTR-H-paniculata_contig12412:3972..40371622928961.163378-UTR-H-paniculata_contig12412:3972..4037Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig12412 3973..4037 -
1691480683.0291088-UTR-H-paniculata_contig12412:3972..40371691480683.0291088-UTR-H-paniculata_contig12412:3972..4037Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig12412 3973..4037 -
1622928961.2235618-UTR-H-paniculata_contig12412:5349..57071622928961.2235618-UTR-H-paniculata_contig12412:5349..5707Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig12412 5350..5707 -
1691480683.0647554-UTR-H-paniculata_contig12412:5349..57071691480683.0647554-UTR-H-paniculata_contig12412:5349..5707Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig12412 5350..5707 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928961.1872485-CDS-H-paniculata_contig12412:4037..41631622928961.1872485-CDS-H-paniculata_contig12412:4037..4163Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig12412 4038..4163 -
1691480683.0402353-CDS-H-paniculata_contig12412:4037..41631691480683.0402353-CDS-H-paniculata_contig12412:4037..4163Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig12412 4038..4163 -
1622928961.2059178-CDS-H-paniculata_contig12412:5163..53491622928961.2059178-CDS-H-paniculata_contig12412:5163..5349Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig12412 5164..5349 -
1691480683.0491748-CDS-H-paniculata_contig12412:5163..53491691480683.0491748-CDS-H-paniculata_contig12412:5163..5349Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig12412 5164..5349 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig12412.1518.1

>prot_H-paniculata_contig12412.1518.1 ID=prot_H-paniculata_contig12412.1518.1|Name=mRNA_H-paniculata_contig12412.1518.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=104bp
MLQSLDLTDPNSRDARRFRSCFGIPYPFVVELIKVVQEGQWFPKREGHVD
VAGRRMVPLELKVLASLQILERGNSFDDIGQLSLMSERVDCPKCVPYILQ
AFR*
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mRNA from alignment at H-paniculata_contig12412:3973..5707-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig12412.1518.1 ID=mRNA_H-paniculata_contig12412.1518.1|Name=mRNA_H-paniculata_contig12412.1518.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1735bp|location=Sequence derived from alignment at H-paniculata_contig12412:3973..5707- (Halopteris paniculata Hal_grac_a_UBK monoicous)
CACCACTCTATGGGCCCTTTTCTCACCGAATTTTTTCATCTGGCGCGCAT CGCGCGATTCGTTGCGCGTCGGAGGCGCGCGCATTTAAATTTAAATCACG GGAGGTGCATACCGCACGCAACAGACTTCTGGCAAGATGAGCGCTCTCCG TCAAGATGCAGTTGAGGTAGCTGTGCGTACAACAGCTGCCTCCACAGTAT TGTCACAGTTGAATGACACTTTAGCGTGTGCATATCTGGTATTGTAGCAA TCTGCGCGTGATGATAGTGATGCTGAAGATGAAGGACATGGGACACACCC AAAGAAGCAGAGAAGGGTGTATGTTCGGTCGCCCTACAGTGAATCGGCGT GGGCAAGGATGCTGCAGTCCCTCGATCTCACGGACCCCAATTCGAGAGAC GCCCGCCGCTTCAGGAGTTGCTTTGGTATACCCTATCCTTTTGTCGTGGA GCTGATAAAGGTGGTGCAAGAAGGACAATGGTTTCCGAAGAGAGAGGGGC ACGTCGATGTCGCCGGTCGCCGAATGGTTCCCCTGGAGCTGAAGGTACGC AGCACGACAACAGGCGATGTCGCAACCAGCGTTTCACGCGATCATGTGCC TGAATAGGAAATGGTTTGTGTTGTACGTGACAGCCAATTTCCCTCAGTTT ACTGTTGCATTGAAATGCTCTCTCTCGCTCTCTCTCTTCGTTTCTTCCAC CATACAGTGGTGCCTCAGTTGTGCTTTACTGTACAGACAGTAAGATCTGT GTCTGTCACTGTGCATGACAGAAGTCGTCCTCCAATTTACAGTTAAGTAG AACTGTGATGATGCGAAGCTAACTTGTATTGTACATTACTGTAGTTTCTG GCCTCATTCCTGTGCAGTACAACCCGTATTGTCCTCGAACATACCGCGGT ACAGAAGGTATGACCCTGCGAAGCTGCAGTACATTGAATAATATAATGGT AGTGACTAAAACAATAAAATAACTTTGTTTTTGAAGAGAATTTTCGTCAT TTCCCCACCAAAGGCTGCCGAATCAGGCAGGGTGCGGGGGATACAAATTG AAGCACTGCACCGTCTCGACAACATGCCATTGGAAAATTTCAAATTGGGC AACATTCAGTTATCAAACAAGTTTAAGGTCCTCATTAAAAAAAACGTGGT ATTTATTAGTGGCCATTGTAAATGTTGTATTGTCTTTTTTTTCGTGTCGG TAAGGTGTGATGCTTACACCTTGTTTCATCATTAGCTTTGCTGAGCAAAG AATATCCGGACCGCCCAATTGACGCGGTGAGCTTGTACCTTAAGCATGCG CGATGCAATATAGGATCCCCCTACATTGTAATCGTATTTTGTTCGATTTT GCTGTGGGTGCATTAGACTCTTTAGTTCTCGTTTATTGGTAAGTATTCGT TTTCAGTGAGGCCATCGCACTGGGAACATGAGCGTCGTCAGCCAATTTCT GCTTAACAATATTTTGCAATCCCAGCCGCCGTCAGTACGGCGAAGTGCTT GATACGATTCCGTAACCCCTACCGTCGCGGTGCTACTACTACAGGTTTTG GCGAGCTTGCAGATTCTGGAGAGAGGGAATAGCTTCGATGATATCGGCCA ACTCTCCTTGATGAGCGAACGAGTCGACTGCCCAAAGTGCGTTCCATACA TTTTGCAAGCGTTTCGGTGAAGATCTTTTTCATGAGTTGATTAAACTCCC TACCGAGGAGGCCCTCGCAGCCACAATGAAGCCGT
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Coding sequence (CDS) from alignment at H-paniculata_contig12412:3973..5707-

>mRNA_H-paniculata_contig12412.1518.1 ID=mRNA_H-paniculata_contig12412.1518.1|Name=mRNA_H-paniculata_contig12412.1518.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=624bp|location=Sequence derived from alignment at H-paniculata_contig12412:3973..5707- (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGCTGCAGTCCCTCGATCTCACGGACCCCAATTCGAGAGACGCCCGCCG
CTTCAGGAGTTGCTTTGGTATACCCTATCCTTTTGTCGTGGAGCTGATAA
AGGTGGTGCAAGAAGGACAATGGTTTCCGAAGAGAGAGGGGCACGTCGAT
GTCGCCGGTCGCCGAATGGTTCCCCTGGAGCTGAAGATGCTGCAGTCCCT
CGATCTCACGGACCCCAATTCGAGAGACGCCCGCCGCTTCAGGAGTTGCT
TTGGTATACCCTATCCTTTTGTCGTGGAGCTGATAAAGGTGGTGCAAGAA
GGACAATGGTTTCCGAAGAGAGAGGGGCACGTCGATGTCGCCGGTCGCCG
AATGGTTCCCCTGGAGCTGAAGGTTTTGGCGAGCTTGCAGATTCTGGAGA
GAGGGAATAGCTTCGATGATATCGGCCAACTCTCCTTGATGAGCGAACGA
GTCGACTGCCCAAAGTGCGTTCCATACATTTTGCAAGCGTTTCGGTGAGT
TTTGGCGAGCTTGCAGATTCTGGAGAGAGGGAATAGCTTCGATGATATCG
GCCAACTCTCCTTGATGAGCGAACGAGTCGACTGCCCAAAGTGCGTTCCA
TACATTTTGCAAGCGTTTCGGTGA
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