mRNA_H-paniculata_contig29560.8039.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig29560.8039.1
Unique NamemRNA_H-paniculata_contig29560.8039.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A6H5JAM1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAM1_9PHAE)

HSP 1 Score: 99.4 bits (246), Expect = 4.530e-22
Identity = 48/86 (55.81%), Postives = 62/86 (72.09%), Query Frame = 1 
Query:   13 CICFGQVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            C     +GI+Q F+H  TAALYP  PL +A+  +L PRFDRA+RRLFRIYD D DGLL D ELN+FQ+ +FRL LS  D+  L+++
Sbjct:  154 CSAVSGMGINQAFYHGRTAALYPRAPLYNAEEGRLCPRFDRAMRRLFRIYDVDRDGLLCDDELNAFQFHAFRLLLSASDLESLRKI 239
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: D8LB08_ECTSI (Miro-related GTP-ase 1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LB08_ECTSI)

HSP 1 Score: 98.6 bits (244), Expect = 8.610e-22
Identity = 47/86 (54.65%), Postives = 63/86 (73.26%), Query Frame = 1 
Query:   13 CICFGQVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            C     +GI+Q F+H  TAALYP  PL +A+  +L PRFDRA+RRLFRI+D D DGLL + ELN+FQ+ +FRL LSD D+  L+++
Sbjct:  154 CSSVSGMGINQAFYHGRTAALYPRAPLYNAEEGRLCPRFDRAMRRLFRIHDVDRDGLLRNDELNAFQFHAFRLLLSDSDLESLRKI 239
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A7S3V0N8_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A7S3V0N8_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 2.340e-18
Identity = 39/81 (48.15%), Postives = 55/81 (67.90%), Query Frame = 1 
Query:   28 QVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
             + +S++FH A  + LYP+ PL D KT +LRP+F +A+ R+FRI+D D DGLL D ELN+FQ   F + L+  DI  +K V
Sbjct:  206 NMNVSEIFHMAQHSVLYPLAPLFDTKTKELRPKFKKALCRVFRIFDMDRDGLLSDSELNNFQERCFGVRLTPTDIEQVKSV 286
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A5J4YMH2_PORPP (Mitochondrial Rho GTPase 1 n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YMH2_PORPP)

HSP 1 Score: 82.4 bits (202), Expect = 4.000e-16
Identity = 36/78 (46.15%), Postives = 54/78 (69.23%), Query Frame = 1 
Query:   37 ISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            +++VF+ A  + L+P  PL D  T+ LRP+   A+ R+FR+ D+DGDGLL D+ELN FQY+ F + L DE++  +K V
Sbjct:  157 VAEVFYFAQKSVLHPTAPLYDVSTHALRPKTVAALTRVFRLCDKDGDGLLNDKELNDFQYQCFNVMLRDEELAGVKNV 234
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A7S2SMD2_9STRA (Hypothetical protein (Fragment) n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2SMD2_9STRA)

HSP 1 Score: 80.1 bits (196), Expect = 2.210e-15
Identity = 38/81 (46.91%), Postives = 52/81 (64.20%), Query Frame = 1 
Query:   28 QVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
             + +S+VF  A  A +YPI PL D +T +L+P+F  A+RR+FRI+D+D DG+L D ELN FQ   F   L   DI  +K V
Sbjct:  171 NINVSEVFSLAQQAVVYPISPLFDTQTLELQPKFKTALRRVFRIFDQDRDGVLSDIELNDFQETCFGARLQPSDIEDVKAV 251
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A7S2WS27_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2WS27_9STRA)

HSP 1 Score: 80.1 bits (196), Expect = 2.590e-15
Identity = 38/81 (46.91%), Postives = 52/81 (64.20%), Query Frame = 1 
Query:   28 QVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
             + +S+VF  A  A +YPI PL D +T +L+P+F  A+RR+FRI+D+D DG+L D ELN FQ   F   L   DI  +K V
Sbjct:  171 NINVSEVFSLAQQAVVYPISPLFDTQTLELQPKFKTALRRVFRIFDQDRDGVLSDIELNDFQETCFGARLQPSDIEDVKAV 251
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: R7QM49_CHOCR (Mitochondrial Rho GTPase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QM49_CHOCR)

HSP 1 Score: 79.3 bits (194), Expect = 4.760e-15
Identity = 36/78 (46.15%), Postives = 53/78 (67.95%), Query Frame = 1 
Query:   37 ISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            IS+VF+ A  A LYP GP+ D +T  L+P    A+RR+F+I D+DGDG L D+ELN FQ+  F ++L   ++  +K+V
Sbjct:  160 ISEVFYFAQKAVLYPTGPVYDVETQSLKPPASAALRRIFKICDKDGDGGLNDKELNDFQFTCFNVHLKPAELDGVKKV 237
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A7S2EWI8_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2EWI8_TRICV)

HSP 1 Score: 79.0 bits (193), Expect = 6.570e-15
Identity = 37/85 (43.53%), Postives = 55/85 (64.71%), Query Frame = 1 
Query:   31 VGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQVGEFNS 285
            + + +VF  A  A LYPI PL D +  Q+ P   RA+ R+FR+YDRD DGLL + ELN+FQ  +F++ L++ D+   K+V   N+
Sbjct:   72 LNVDEVFLKARQAVLYPITPLYDLEEGQIAPNCRRALTRIFRMYDRDNDGLLSNAELNAFQKRAFQVPLAERDLTGWKKVVSKNN 156
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: A0A396HPX0_MEDTR (Mitochondrial Rho GTPase n=1 Tax=Medicago truncatula TaxID=3880 RepID=A0A396HPX0_MEDTR)

HSP 1 Score: 77.4 bits (189), Expect = 2.260e-14
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1 
Query:    7 FSCICFGQVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            F C  +  + + +VF+ A  AALYP+ PL D K++ L+PR +RA++R+F + D D DG L D ELN+FQ   F   +   +I  +K+V
Sbjct:  120 FECSAYRLIQVLEVFYFAQKAALYPMAPLFDQKSHTLKPRCERALKRIFTLCDHDRDGALSDAELNNFQAVCFNAPMQPWEIANVKKV 207
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BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Match: G7KF84_MEDTR (Mitochondrial Rho GTPase n=2 Tax=Medicago truncatula TaxID=3880 RepID=G7KF84_MEDTR)

HSP 1 Score: 77.4 bits (189), Expect = 2.260e-14
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1 
Query:    7 FSCICFGQVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRLFRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQV 270
            F C  +  + + +VF+ A  AALYP+ PL D K++ L+PR +RA++R+F + D D DG L D ELN+FQ   F   +   +I  +K+V
Sbjct:  157 FECSAYRLIQVLEVFYFAQKAALYPMAPLFDQKSHTLKPRCERALKRIFTLCDHDRDGALSDAELNNFQAVCFNAPMQPWEIANVKKV 244
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig29560.8039.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JAM1_9PHAE4.530e-2255.81Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LB08_ECTSI8.610e-2254.65Miro-related GTP-ase 1 n=1 Tax=Ectocarpus siliculo... [more]
A0A7S3V0N8_9STRA2.340e-1848.15Hypothetical protein (Fragment) n=1 Tax=Aplanochyt... [more]
A0A5J4YMH2_PORPP4.000e-1646.15Mitochondrial Rho GTPase 1 n=1 Tax=Porphyridium pu... [more]
A0A7S2SMD2_9STRA2.210e-1546.91Hypothetical protein (Fragment) n=1 Tax=labyrinthu... [more]
A0A7S2WS27_9STRA2.590e-1546.91Hypothetical protein n=1 Tax=labyrinthulid quahog ... [more]
R7QM49_CHOCR4.760e-1546.15Mitochondrial Rho GTPase n=1 Tax=Chondrus crispus ... [more]
A0A7S2EWI8_TRICV6.570e-1543.53Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
A0A396HPX0_MEDTR2.260e-1440.91Mitochondrial Rho GTPase n=1 Tax=Medicago truncatu... [more]
G7KF84_MEDTR2.260e-1440.91Mitochondrial Rho GTPase n=2 Tax=Medicago truncatu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig29560contigH-paniculata_contig29560:502..1682 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score98.6
Seed ortholog evalue1.9e-18
Seed eggNOG ortholog2880.D8LB08
Preferred nameRHOT2
KEGG koko:K06883,ko:K07870,ko:K07871
KEGG Pathwayko04137,ko04214,map04137,map04214
Hectar predicted targeting categorysignal peptide
GOsGO:0000001,GO:0000003,GO:0000166,GO:0000226,GO:0000287,GO:0000902,GO:0000904,GO:0001101,GO:0001505,GO:0001882,GO:0001883,GO:0003006,GO:0003674,GO:0003824,GO:0003924,GO:0005488,GO:0005509,GO:0005515,GO:0005525,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005741,GO:0005783,GO:0005829,GO:0005886,GO:0006464,GO:0006508,GO:0006807,GO:0006810,GO:0006839,GO:0006915,GO:0006928,GO:0006996,GO:0007005,GO:0007006,GO:0007010,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007275,GO:0008088,GO:0008150,GO:0008152,GO:0008219,GO:0008637,GO:0009653,GO:0009719,GO:0009725,GO:0009737,GO:0009790,GO:0009791,GO:0009793,GO:0009826,GO:0009856,GO:0009860,GO:0009932,GO:0009966,GO:0009987,GO:0010033,GO:0010154,GO:0010570,GO:0010646,GO:0010821,GO:0010970,GO:0012501,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0016049,GO:0016192,GO:0016462,GO:0016579,GO:0016787,GO:0016817,GO:0016818,GO:0017076,GO:0017111,GO:0019001,GO:0019538,GO:0019725,GO:0019867,GO:0019896,GO:0022406,GO:0022414,GO:0023051,GO:0023052,GO:0030154,GO:0030705,GO:0031090,GO:0031224,GO:0031300,GO:0031301,GO:0031306,GO:0031307,GO:0031966,GO:0031967,GO:0031968,GO:0031975,GO:0032386,GO:0032501,GO:0032502,GO:0032549,GO:0032550,GO:0032553,GO:0032555,GO:0032561,GO:0032592,GO:0032865,GO:0032879,GO:0032886,GO:0032989,GO:0032991,GO:0033043,GO:0033993,GO:0034640,GO:0034643,GO:0035639,GO:0035794,GO:0036094,GO:0036211,GO:0040007,GO:0040008,GO:0042221,GO:0042592,GO:0043167,GO:0043168,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044232,GO:0044233,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044432,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0044706,GO:0046872,GO:0046902,GO:0046907,GO:0046928,GO:0047497,GO:0048308,GO:0048311,GO:0048316,GO:0048468,GO:0048489,GO:0048583,GO:0048588,GO:0048589,GO:0048608,GO:0048731,GO:0048856,GO:0048868,GO:0048869,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050804,GO:0050896,GO:0051046,GO:0051049,GO:0051056,GO:0051128,GO:0051179,GO:0051234,GO:0051270,GO:0051588,GO:0051640,GO:0051641,GO:0051646,GO:0051648,GO:0051649,GO:0051650,GO:0051654,GO:0051656,GO:0051704,GO:0051716,GO:0055081,GO:0055088,GO:0055091,GO:0060237,GO:0060341,GO:0060560,GO:0060632,GO:0061024,GO:0061458,GO:0065007,GO:0065008,GO:0070646,GO:0070647,GO:0071704,GO:0071840,GO:0071944,GO:0072384,GO:0090559,GO:0097159,GO:0097190,GO:0097305,GO:0097345,GO:0097367,GO:0097479,GO:0097480,GO:0098573,GO:0098588,GO:0098796,GO:0098798,GO:0098799,GO:0098805,GO:0098930,GO:0099003,GO:0099098,GO:0099111,GO:0099177,GO:0140056,GO:1900428,GO:1901265,GO:1901363,GO:1901564,GO:1901700,GO:1902108,GO:1902110,GO:1902513,GO:1902531,GO:1902686,GO:1903338,GO:1903530,GO:1905710,GO:1990456
EggNOG free text desc.GTPase activity
EggNOG OGsCOG1100@1,KOG1707@2759
COG Functional cat.F
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03029,ko04031
Exons2
Model size318
Cds size318
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929902.7778592-CDS-H-paniculata_contig29560:501..6061622929902.7778592-CDS-H-paniculata_contig29560:501..606Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig29560 502..606 +
1691481062.2985125-CDS-H-paniculata_contig29560:501..6061691481062.2985125-CDS-H-paniculata_contig29560:501..606Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig29560 502..606 +
1622929902.7967777-CDS-H-paniculata_contig29560:1469..16821622929902.7967777-CDS-H-paniculata_contig29560:1469..1682Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig29560 1470..1682 +
1691481062.3119118-CDS-H-paniculata_contig29560:1469..16821691481062.3119118-CDS-H-paniculata_contig29560:1469..1682Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig29560 1470..1682 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig29560.8039.1prot_H-paniculata_contig29560.8039.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig29560 502..1682 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig29560.8039.1

>prot_H-paniculata_contig29560.8039.1 ID=prot_H-paniculata_contig29560.8039.1|Name=mRNA_H-paniculata_contig29560.8039.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=106bp
LLFSCICFGQVGISQVFHHALTAALYPIGPLLDAKTNQLRPRFDRAIRRL
FRIYDRDGDGLLGDQELNSFQYESFRLNLSDEDIVVLKQVGEFNSGGCNG
DNGCL*
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mRNA from alignment at H-paniculata_contig29560:502..1682+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig29560.8039.1 ID=mRNA_H-paniculata_contig29560.8039.1|Name=mRNA_H-paniculata_contig29560.8039.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1181bp|location=Sequence derived from alignment at H-paniculata_contig29560:502..1682+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTGCTTTTCTCCTGTATCTGCTTTGGGCAGGTTGGCATTTCCCAGGTGTT
TCACCATGCGTTGACGGCGGCTCTCTATCCGATAGGCCCGCTCTTGGATG
CCAAGGTACGAGTATGTACCACGCTGTGGTTGGTCATATTCCAGGGCATG
CTATCACCCCCCGATGATATAAGGACAGGCATAACAATTTTGAGGCGCAT
TTGGCGACGTAGGAAAGAGCGATTGCCCATCGGAAATTGCTCTGTTAAAA
CAAAGATCTGTCCTCCCAAGGACGCGTGGGTCAACGGTCTGGCAACTTCG
GCATGCAATACATGAGCACGCTTATTTTTCAACCTCGATTGTCTGCACAA
GGGTTAAGTGTGGGTGGTTGTGTATGCGGTGACGTAGGACGTCTGTTGCG
CCGTTGGATGAGATGCCGAAGAATTGTTTCATTGGATAGTTGGACGAAAA
TTAGGTAGTCATGGGCAGAAAAGATGGTGTGTCCAACTCCGTGCAGATGG
TCAAGACGAGATGAGATGCTGAGCAGCCACGCTGCCAGAGATTATAAGCG
TATCAGTCTTATGCACGTCCGACGGAACAGTTTTTCCTTTATTGTAGATA
TATTCCAACACGTGTGTATATACACTTTTTCTTATAATGGATTATGTTCT
AAACCATGTACATACAGCTCGATTTTTTTTCTTGAAAGGTCAATATGTTT
AGTTGGGTTTGAGACTCTCTCCCCCGAAATAAGTGGTTCTTATCGCGATT
AACCAGTAGCAGGACTGTTTTTCTCTTCTGAATTAAATTAGGCCGCTTGT
TATTGGCGTCGTGTTTTCCTATCGGACGGGATATTTGGACGTTTCAGTAC
AGTTATCTATAGCTGGTGAAAAATTCCGACGACTGGCTCAAAAACGGTGT
TTCGATGATCACATAAGAAGATAATAATTTTGTTTTGTTTTGTTTTTTTC
GTATTTTTGCACATAAAGACGAACCAGCTACGGCCCCGGTTCGACAGAGC
TATTCGTAGATTGTTTCGCATCTACGACAGAGATGGAGATGGTCTGCTGG
GTGACCAGGAGCTGAACTCGTTCCAATATGAGTCTTTTCGGCTTAACCTT
TCGGACGAAGACATTGTTGTGCTGAAGCAGGTGGGCGAATTCAATTCCGG
TGGCTGTAATGGCGATAACGGTTGTCTGTAA
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Coding sequence (CDS) from alignment at H-paniculata_contig29560:502..1682+

>mRNA_H-paniculata_contig29560.8039.1 ID=mRNA_H-paniculata_contig29560.8039.1|Name=mRNA_H-paniculata_contig29560.8039.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=636bp|location=Sequence derived from alignment at H-paniculata_contig29560:502..1682+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTGCTTTTCTCCTGTATCTGCTTTGGGCAGGTTGGCATTTCCCAGGTGTT
TCACCATGCGTTGACGGCGGCTCTCTATCCGATAGGCCCGCTCTTGGATG
CCAAGCTGCTTTTCTCCTGTATCTGCTTTGGGCAGGTTGGCATTTCCCAG
GTGTTTCACCATGCGTTGACGGCGGCTCTCTATCCGATAGGCCCGCTCTT
GGATGCCAAGACGAACCAGCTACGGCCCCGGTTCGACAGAGCTATTCGTA
GATTGTTTCGCATCTACGACAGAGATGGAGATGGTCTGCTGGGTGACCAG
GAGCTGAACTCGTTCCAATATGAGTCTTTTCGGCTTAACCTTTCGGACGA
AGACATTGTTGTGCTGAAGCAGGTGGGCGAATTCAATTCCGGTGGCTGTA
ATGGCGATAACGGTTGTCTGTAAACGAACCAGCTACGGCCCCGGTTCGAC
AGAGCTATTCGTAGATTGTTTCGCATCTACGACAGAGATGGAGATGGTCT
GCTGGGTGACCAGGAGCTGAACTCGTTCCAATATGAGTCTTTTCGGCTTA
ACCTTTCGGACGAAGACATTGTTGTGCTGAAGCAGGTGGGCGAATTCAAT
TCCGGTGGCTGTAATGGCGATAACGGTTGTCTGTAA
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