mRNA_H-paniculata_contig21359.5611.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig21359.5611.1
Unique NamemRNA_H-paniculata_contig21359.5611.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: D8LK92_ECTSI (C2 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK92_ECTSI)

HSP 1 Score: 198 bits (504), Expect = 4.400e-57
Identity = 99/107 (92.52%), Postives = 103/107 (96.26%), Query Frame = 1 
Query:    1 KDLTRFGRARKAVKKRTKELDAGAAHLRSMLHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHLQ 321
            KDL+RFGRARKAVKKRTKE+DAGAAHLRSML GHLGPDFEQVISLRRMQMRVK+FLRRSLL VYDHEEGES AE TGIGKVVANKGGLVCKLRL+GTTLCFVSCHLQ
Sbjct:  108 KDLSRFGRARKAVKKRTKEMDAGAAHLRSMLSGHLGPDFEQVISLRRMQMRVKIFLRRSLLDVYDHEEGESAAENTGIGKVVANKGGLVCKLRLRGTTLCFVSCHLQ 214
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A6H5KKF7_9PHAE (C2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KKF7_9PHAE)

HSP 1 Score: 197 bits (501), Expect = 1.620e-56
Identity = 98/107 (91.59%), Postives = 103/107 (96.26%), Query Frame = 1 
Query:    1 KDLTRFGRARKAVKKRTKELDAGAAHLRSMLHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHLQ 321
            KDL+RFGRA+KAVKKRTKE+DAGAAHLRSML GHLGPDFEQVISLRRMQMRVK+FLRRSLL VYDHEEGES AE TGIGKVVANKGGLVCKLRL+GTTLCFVSCHLQ
Sbjct:   49 KDLSRFGRAKKAVKKRTKEMDAGAAHLRSMLSGHLGPDFEQVISLRRMQMRVKIFLRRSLLDVYDHEEGESAAENTGIGKVVANKGGLVCKLRLRGTTLCFVSCHLQ 155
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A7S3Y699_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y699_HETAK)

HSP 1 Score: 84.0 bits (206), Expect = 4.710e-18
Identity = 44/85 (51.76%), Postives = 60/85 (70.59%), Query Frame = 1 
Query:   67 GAAHLRSMLHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHLQ 321
            G   L++++  HLG DF  V   RR QM+++V++RR+L S     + +  AE TGIG ++ANKGGLVCKL+L  TTLCFVSCHL+
Sbjct:   22 GYKRLKTIIESHLGSDFRCVGKHRRGQMQLRVYVRRALASQVSGVQID--AENTGIGHILANKGGLVCKLQLGHTTLCFVSCHLE 104
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A6T5RE32_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6T5RE32_HETAK)

HSP 1 Score: 70.9 bits (172), Expect = 4.600e-12
Identity = 39/78 (50.00%), Postives = 51/78 (65.38%), Query Frame = 1 
Query:   88 MLHGHLGPDFEQVISLRRMQMRVKVFLRRSLL-SVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            M   HLGPDF  + S +R  M++++++R  L   V D E G   AE TGIG ++ANKGG  C L L+GT+L FVSCHL
Sbjct:  150 MFADHLGPDFVLISSSKRGFMQLRIYVRAELAPEVTDLEVG---AENTGIGGIIANKGGQACALTLRGTSLVFVSCHL 224
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A7S2RZJ1_9STRA (Hypothetical protein n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RZJ1_9STRA)

HSP 1 Score: 68.9 bits (167), Expect = 2.190e-11
Identity = 40/84 (47.62%), Postives = 52/84 (61.90%), Query Frame = 1 
Query:   67 GAAHLRSMLHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            G  HL   L   LG +F  +  +RR+QMR++V++R SL+   D EE E  AE TG   V ANKGG V KL++  TT+ FVS HL
Sbjct:  149 GDKHLLRSLQAQLGSEFTLLQRVRRLQMRIRVYVRDSLMK--DIEEVEVGAENTGFAGVGANKGGQVVKLKILNTTIMFVSSHL 230
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A7I4FBI4_PHYPA (C2 domain-containing protein n=4 Tax=Physcomitrium patens TaxID=3218 RepID=A0A7I4FBI4_PHYPA)

HSP 1 Score: 58.2 bits (139), Expect = 1.350e-7
Identity = 31/76 (40.79%), Postives = 44/76 (57.89%), Query Frame = 1 
Query:   91 LHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            L  H G  ++ V ++ R QMR+ V +R          E +S +E TG+G V+ANKGG+   L+   T LCFV+CHL
Sbjct:  280 LKSHFGERYKLVHAISRGQMRLVVLVREDAEKAIS--EVDSDSEATGVGNVMANKGGVCISLKFWDTGLCFVNCHL 353
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A5D6XVT6_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XVT6_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 1.930e-7
Identity = 39/118 (33.05%), Postives = 66/118 (55.93%), Query Frame = 1 
Query:    7 LTRFGRARKAVKKRTKELDAGAAHLRS------------MLHGHLGPDFEQVISLRRMQMRVKVFL--RRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            + +  RA+K+++K +  +   +++LR              L+ HLG  +  V  +  M+MR+ V++  R ++ S+      E  +  TG+G V+ NKGGLV KL ++ T+LCFVSCHL
Sbjct: 1420 IVKRSRAKKSIRKVSNLVRQVSSNLRESVADALDYPFNKQLYHHLGESYGLVGKVELMEMRLFVYVHSRHTVSSI------EKISVPTGLGSVLGNKGGLVFKLVVQDTSLCFVSCHL 1531
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A8K1CJL7_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CJL7_PYTOL)

HSP 1 Score: 57.4 bits (137), Expect = 2.480e-7
Identity = 31/73 (42.47%), Postives = 43/73 (58.90%), Query Frame = 1 
Query:  100 HLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            HLG  +  V  +  ++MR+ VF+     S +     E  +  TG+G V+ NKGGL+ KL L  T+LCFVSCHL
Sbjct:  189 HLGEQYVLVAKVELLEMRLLVFVH----SKHRVSGIEKHSIATGLGSVIGNKGGLILKLVLHNTSLCFVSCHL 257
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BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: K3X4F6_GLOUD (C2 domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X4F6_GLOUD)

HSP 1 Score: 55.5 bits (132), Expect = 1.190e-6
Identity = 36/118 (30.51%), Postives = 64/118 (54.24%), Query Frame = 1 
Query:    7 LTRFGRARKAVKKRTKELDAGAAHLRS------------MLHGHLGPDFEQVISLRRMQMRVKVFL--RRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            + +  R +K++++ +K +   +A+LR              L+ HLG  F  V  +  M+MR+ +++  + S+  +      E     TG+G V+ NKGGL+ K+ ++ T+LCFVSCHL
Sbjct:  206 IVKRSRTKKSIRRVSKLVRQVSANLRDSVAEVLDYPFNRQLYLHLGDKFALVGKVELMEMRLFMYVQTKHSVTGI------EKATVPTGLGSVLGNKGGLIFKMVVQNTSLCFVSCHL 317
[Close]
BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Match: A0A2K1L0R9_PHYPA (C2 domain-containing protein n=2 Tax=Physcomitrium patens TaxID=3218 RepID=A0A2K1L0R9_PHYPA)

HSP 1 Score: 54.3 bits (129), Expect = 3.030e-6
Identity = 30/76 (39.47%), Postives = 42/76 (55.26%), Query Frame = 1 
Query:   91 LHGHLGPDFEQVISLRRMQMRVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLCFVSCHL 318
            L  H G  ++ V +  R QMR+ V +R          E +S +E TG+G V+ANKGG+    +   T LCFV+CHL
Sbjct:  280 LKSHFGERYKLVHATSRGQMRLVVLVRDDAEKAIS--EVDSDSEATGVGNVMANKGGVCIAFKFWDTGLCFVNCHL 353
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig21359.5611.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 16
Match NameE-valueIdentityDescription
D8LK92_ECTSI4.400e-5792.52C2 domain-containing protein n=1 Tax=Ectocarpus si... [more]
A0A6H5KKF7_9PHAE1.620e-5691.59C2 domain-containing protein n=1 Tax=Ectocarpus sp... [more]
A0A7S3Y699_HETAK4.710e-1851.76Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A6T5RE32_HETAK4.600e-1250.00Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S2RZJ1_9STRA2.190e-1147.62Hypothetical protein n=2 Tax=Rhizochromulina marin... [more]
A0A7I4FBI4_PHYPA1.350e-740.79C2 domain-containing protein n=4 Tax=Physcomitrium... [more]
A0A5D6XVT6_9STRA1.930e-733.05Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
A0A8K1CJL7_PYTOL2.480e-742.47Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
K3X4F6_GLOUD1.190e-630.51C2 domain-containing protein n=1 Tax=Globisporangi... [more]
A0A2K1L0R9_PHYPA3.030e-639.47C2 domain-containing protein n=2 Tax=Physcomitrium... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig21359contigH-paniculata_contig21359:1560..3841 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score198.7
Seed ortholog evalue1.3e-48
Seed eggNOG ortholog2880.D8LK92
Preferred nameINPP5E
KEGG rclassRC00005,RC00078,RC00482
KEGG koko:K07151,ko:K20278
KEGG ReactionR04216,R04404,R05976,R09827
KEGG Pathwayko00510,ko00513,ko00562,ko01100,ko04070,ko04141,map00510,map00513,map00562,map01100,map04070,map04141
KEGG ModuleM00072
Hectar predicted targeting categoryother localisation
EggNOG free text desc.phosphatidylinositol dephosphorylation
EggNOG OGsCOG5411@1,KOG0565@2759
EC2.4.99.18,3.1.3.36
COG Functional cat.T
CAZyGT66
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Exons4
Model size321
Cds size321
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig21359.5611.1prot_H-paniculata_contig21359.5611.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig21359 1560..3841 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929573.637013-CDS-H-paniculata_contig21359:1559..15921622929573.637013-CDS-H-paniculata_contig21359:1559..1592Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 1560..1592 +
1691480919.6432848-CDS-H-paniculata_contig21359:1559..15921691480919.6432848-CDS-H-paniculata_contig21359:1559..1592Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 1560..1592 +
1622929573.6537657-CDS-H-paniculata_contig21359:2529..26191622929573.6537657-CDS-H-paniculata_contig21359:2529..2619Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 2530..2619 +
1691480919.6564896-CDS-H-paniculata_contig21359:2529..26191691480919.6564896-CDS-H-paniculata_contig21359:2529..2619Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 2530..2619 +
1622929573.672185-CDS-H-paniculata_contig21359:3093..32001622929573.672185-CDS-H-paniculata_contig21359:3093..3200Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 3094..3200 +
1691480919.666276-CDS-H-paniculata_contig21359:3093..32001691480919.666276-CDS-H-paniculata_contig21359:3093..3200Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 3094..3200 +
1622929573.6868722-CDS-H-paniculata_contig21359:3750..38411622929573.6868722-CDS-H-paniculata_contig21359:3750..3841Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 3751..3841 +
1691480919.676157-CDS-H-paniculata_contig21359:3750..38411691480919.676157-CDS-H-paniculata_contig21359:3750..3841Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig21359 3751..3841 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig21359.5611.1

>prot_H-paniculata_contig21359.5611.1 ID=prot_H-paniculata_contig21359.5611.1|Name=mRNA_H-paniculata_contig21359.5611.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=107bp
KDLTRFGRARKAVKKRTKELDAGAAHLRSMLHGHLGPDFEQVISLRRMQM
RVKVFLRRSLLSVYDHEEGESTAEITGIGKVVANKGGLVCKLRLKGTTLC
FVSCHLQ
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mRNA from alignment at H-paniculata_contig21359:1560..3841+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig21359.5611.1 ID=mRNA_H-paniculata_contig21359.5611.1|Name=mRNA_H-paniculata_contig21359.5611.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=2282bp|location=Sequence derived from alignment at H-paniculata_contig21359:1560..3841+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
AAAGACCTGACCCGCTTCGGCCGCGCTAGGAAGGTGAAAATTACTTTAGT
TCAGTTTTTATCAGGGGTATGCCATACGTCGAGGGGGGGGAGCATGTGAT
GGTAAATAGCCCCCCTTGTACCCGCCGGGATTTTCGTTCGTAACGCAACC
CACCTGCTACAGTTGGTAGAACTATCGCTCTAAGGCTCATATAATATAAG
TGTCCACTCTACTCCATGTTGAGGTAATGGAGCTGACTGCAAACTTTAGT
TGTGTGCATGTATCAGCAAAAATTGGACCAATAAGGGGCTGAGGGTATAT
AGCCCAGCTGGAACATAGCTGCGTACACAGTGCGTTATGTCACGAAAACT
ATACCAAGAAAAAGGGGGGTGTACTCGTGTTTTGTTTCTCATGCCAAAGC
TGTTCGGCCATCGACCACGCGTCATCTTGAGGAGGAAGGTGCTTTGTCCC
TGTAGATGGATAAAGCGACTTTCCGATGAGTACGCGCTCCCTTCTTTTAA
TTTTTTGCTGCCAAAGAAATATATACCTCGAAAGGATTGTTTACAGTTCC
CACCTGTGCTGCAATCCTTTACCTGTCCTGTCCACGTCTATACACACGTC
CCGAATTGTAACCATAGCTTATTTACTTACTCCTCTTGCCCATCGTCTGT
ATGCTGTATTCTGATTTCGTGTTTTTTTGCTTCACCAAAGTCCCCGCCGC
CGGTGACTGATATATATATATTTTTTATCAATATTTTTAACCACACTCGT
GGTGATGGGTAATGGATGCTTCATAATCCACGGTCCGTGCCACTCCGTGT
CGTCCCCTTGTTGGGATGCACTAGCTCGCTGGCTCACTGGCTCGCCACTT
GCAGTCGTTTGGTCAATGCGGGTTGCCCTAAGAATGCCTATGTTGGGGGC
ATCGCCGTTGTGTGGTCGGGTGTTGCTGTGACTGTGGCTGTACTTTTTGG
TTTATTTCGCGTTAGAATAGGCGGTGAAAAAGAGGACGAAGGAGTTAGAC
GCTGGTGCGGCGCATCTGAGGTCCATGCTCCATGGACACCTAGGTCCGGA
CTTTGAGCAGGTGCGTGAGCGAGTTTACCTCGGCCGTCTGTTATTTGGTC
TTCATGTATTGTTGGTTATCATAGTCGTCATCATCTATCACCTTGGGTAC
TGTTGACTAAAACAAGGTTCAATTACCCAGGAAAAATGTTGGAAATAGCG
TAATCAATGCTTACGTTGTCTCGGGCGAGCGTGTGTGGGCGGGCGGGCGG
GCGGGGGGGGGTCTTACTTGCCATAACATGCAGTACAGTAAGTCTCTGTA
CTCATGCTGTAAACTCAATCAACCCACACGAGATTATGGCTAGCTGTGTG
TCCTTACCAGCAAAATATGCACAGACGAGAAAAATACGAGGGAAGGGGGA
GGGGAGCGTGCGTATATTTACTTGGTGTCATTCTTCTGACGTATTCGTAC
GGTAAATTACCATCTGGTGAGCCAACCTCCTGCGTATGTTGCGTACATGT
GTGCATGTGGTGTTGTTTTGGGGTCCTCCGATAGGTGATCTCGTTGCGAA
GGATGCAGATGAGAGTCAAGGTGTTCCTCCGGAGATCTTTGCTATCCGTC
TACGACCACGAGGAGGGGGAGTCTACGGCCGAGATCACAGGGTAAGAAAT
ATCACATGGGGAAGAAAGGGTGTTGCTCTATTGTAGTCTAAAGAGCAGCA
CTGTGCTGAATAACGATCACATGGATGTAGGAAACCGACATGTATTATAT
ATATATATATATATGTAAATATGTATTGAAGGCCTTGCTCAATAGTCTAA
AACATACTACTTTTTGAGATAGTGATGACATGCTAGGTAGATTTTTGTGG
GACGCTGTAGGGATAAAACCCGCTTTGTACATATGTTTTTTTCAGTTATA
GAGGTAGCCTTATAGAAGATGTGATCCATTTCTCCCATCTCCCACTATTG
ATGCCACGTACGCAAACCTGACCATGCTACATTCGAATCGGTCCGAGTCG
AGCACGAGTTCCTTTTCTGCCTGTAGGTGGACAACGCCACCAAGGTATTG
GTCACTGTTCATTGGTGCGGATGGATGACTGTAGTTGATTGCACGCACCT
TTGAAGTGCATTTCGATTGGAAGTACAACACGTGCATATCAATAGATCCC
CCATCCACCCCTCTCTTTTTCCCCTGCTGCTTGTGTGGCAGCATCGGTAA
GGTGGTAGCGAACAAGGGTGGCTTGGTGTGCAAGCTGAGACTCAAGGGCA
CCACGCTCTGCTTCGTCAGCTGCCACTTGCAG
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Coding sequence (CDS) from alignment at H-paniculata_contig21359:1560..3841+

>mRNA_H-paniculata_contig21359.5611.1 ID=mRNA_H-paniculata_contig21359.5611.1|Name=mRNA_H-paniculata_contig21359.5611.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=642bp|location=Sequence derived from alignment at H-paniculata_contig21359:1560..3841+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
AAAGACCTGACCCGCTTCGGCCGCGCTAGGAAGAAAGACCTGACCCGCTT
CGGCCGCGCTAGGAAGGCGGTGAAAAAGAGGACGAAGGAGTTAGACGCTG
GTGCGGCGCATCTGAGGTCCATGCTCCATGGACACCTAGGTCCGGACTTT
GAGCAGGCGGTGAAAAAGAGGACGAAGGAGTTAGACGCTGGTGCGGCGCA
TCTGAGGTCCATGCTCCATGGACACCTAGGTCCGGACTTTGAGCAGGTGA
TCTCGTTGCGAAGGATGCAGATGAGAGTCAAGGTGTTCCTCCGGAGATCT
TTGCTATCCGTCTACGACCACGAGGAGGGGGAGTCTACGGCCGAGATCAC
AGGGTGATCTCGTTGCGAAGGATGCAGATGAGAGTCAAGGTGTTCCTCCG
GAGATCTTTGCTATCCGTCTACGACCACGAGGAGGGGGAGTCTACGGCCG
AGATCACAGGCATCGGTAAGGTGGTAGCGAACAAGGGTGGCTTGGTGTGC
AAGCTGAGACTCAAGGGCACCACGCTCTGCTTCGTCAGCTGCCACTTGCA
GCATCGGTAAGGTGGTAGCGAACAAGGGTGGCTTGGTGTGCAAGCTGAGA
CTCAAGGGCACCACGCTCTGCTTCGTCAGCTGCCACTTGCAG
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