EsuBft1180_3a-0001 (mRNA) Ectocarpus subulatus male Bft15b

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NameEsuBft1180_3a-0001
Unique NameEsuBft1180_3a-0001
TypemRNA
OrganismEctocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Scaffold_106contigScaffold_106:178870..183758 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
OGS1.0 of Ectocarpus subulatus male Bft15b2020-06-19
Properties
Property NameValue
Seed ortholog746128.CADAFUBP00002101
ProteomeIdEsuBft1180_3
Protein domainsSIGNAL_PEPTIDE_C_REGION
Protein domainsIPR006140
Protein domainsIPR029753
Protein domainsPTHR10996
Protein domainsSIGNAL_PEPTIDE_N_REGION
Protein domainsSignalP-noTM
Protein domainsIPR016040
Protein domainsSSF52283
Protein domainsSIGNAL_PEPTIDE_H_REGION
Protein domainsSIGNAL_PEPTIDE
Protein domainsSSF51735
Protein domainsIPR006139
Protein domainsNON_CYTOPLASMIC_DOMAIN
Ownerapollo_admin@sb-roscoff.fr
Noted-3-phosphoglycerate dehydrogenase os:methanocaldococcus jannaschii (strain atcc 43067 dsm 2661 [Automatic annotation]
Nb exon7
Max annot lvl4751|Fungi
Length1703
Hectar predicted targeting categorysignal peptide
Evalue1.7e-72
EggNOG OGsCOG0111@1|root,KOG0068@2759|Eukaryota,38GJA@33154|Opisthokonta,3NVPH@4751|Fungi,3QN9K@4890|Ascomycota,20G9M@147545|Eurotiomycetes,3S67S@5042|Eurotiales
Date last modified2016-06-14
Date creation2016-06-14
KEGG rclassRC00031
KEGG koko:K00058
KEGG ReactionR01513
KEGG Pathwayko00260,ko00680,ko01100,ko01120,ko01130,ko01200,ko01230,map00260,map00680,map01100,map01120,map01130,map01200,map01230
KEGG ModuleM00020
EC1.1.1.399,1.1.1.95
DescriptionD-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
COG categoryE
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Cross References
External references for this mRNA
DatabaseAccession
GO0007399
GO0016616
GO0009069
GO0051287
GO0055114
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3aEsuBft1180_3aEctocarpus subulatus male Bft15bgeneScaffold_106 178870..183758 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3a-0001EsuBft1180_3Ectocarpus subulatus male Bft15bpolypeptideScaffold_106 178923..183272 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3a-0001-exonagat-exon-58697Ectocarpus subulatus male Bft15bexonScaffold_106 178870..179075 +
EsuBft1180_3a-0001-exonagat-exon-58696Ectocarpus subulatus male Bft15bexonScaffold_106 179627..179650 +
EsuBft1180_3a-0001-exonEsuBft1180_3a-0001-exonEctocarpus subulatus male Bft15bexonScaffold_106 181118..181352 +
EsuBft1180_3a-0001-exonagat-exon-58700Ectocarpus subulatus male Bft15bexonScaffold_106 181596..181750 +
EsuBft1180_3a-0001-exonagat-exon-58698Ectocarpus subulatus male Bft15bexonScaffold_106 182246..182555 +
EsuBft1180_3a-0001-exonagat-exon-58695Ectocarpus subulatus male Bft15bexonScaffold_106 182696..182798 +
EsuBft1180_3a-0001-exonagat-exon-58699Ectocarpus subulatus male Bft15bexonScaffold_106 183089..183758 +


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3a-0001-exonagat-five_prime_utr-1146Ectocarpus subulatus male Bft15bfive_prime_UTRScaffold_106 178870..178922 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3a-0001-cdsEsuBft1180_3a-0001-cdsEctocarpus subulatus male Bft15bCDSScaffold_106 178923..179075 +


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
EsuBft1180_3a-0001-exonagat-three_prime_utr-1749Ectocarpus subulatus male Bft15bthree_prime_UTRScaffold_106 183273..183758 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>EsuBft1180_3a-0001 ID=EsuBft1180_3a-0001|Name=EsuBft1180_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=1703bp|location=Sequence derived from alignment at Scaffold_106:178870..183758+ (Ectocarpus subulatus male Bft15b)|Notes=Excludes all bases but those of type(s): exon.  
TGCATCTACCACTGTAAAACGATAGAGCGTTGTTTGCATTGCAGCTGCAA
CCGATGCGATCCACAGGCGTAGCCCTCGCGTTCCTCGCGGTACGCTCCAG
GCTTACATGGCTCGGTCCACGTCAGGGTATATACGGAGCTCTTCGTGCAA
CAGACACGACACGAAGACACATTTTGGTCCTAGACCCGGCGTCCCCTGCA
TACCAGGGGATTGAGACAGCTGGATACCAGGTGACCGCCGCGCATGGTTT
AAGCGATCCCGAGCTTGAGCAAGTCCTCCCAGCGTATGACGCGGTCGTGG
TTCGTTCGGCCAATCAAATCACTCGCAACATGCTAGTCGCGAGTCCCCGG
GTGGCGATAGTAGGGCGTGCGGGGTCTGGGGTGGACAATATCGACCTGAA
GGCGGCCTCCGAGCTCGGGGTACCTGTTGTGAATGCTCCCACGGGAAACT
CAGGGTCTGTTGCAGAGTTGGTAATGGGCTTGATGCTCGGCGTGTCACGG
AGCATTTCGCCAGCGCACGACACCACAAGACGCGGGGAATGGGCTAAGAG
CAATTTCACTGGCCGCACACTCAGCGGAAAGACACTGGGAATTGTAGGCT
TCGGTTCTGTGGGCATAGCGCTGGCAAGACTGGCCGTGGCTTTCGGCATG
GTGGTAATGATAGCAGAAGGGTCGAGTCCGACGCCTTGCTCGGGGAGAGC
TGAGGAAATCGACCCACGCGCATCCGAGAGGGAGGGTATGGCAAGCCAAG
CAGGGGTAACGTTGCCTCCTCTGCCGCTCGACGAACTCTTGTCGAAGAGC
GACTTTTTTTCTGTGCACTGCCCGCTGACGGCGGACACGAAGGGCCTGAT
TTCTAGACGCGAACTGGCCCTGATGAAGCCATCTGCCGTGGTTATCAATG
CGGCGAGAGGAGGAGTGGTGGACGAATTAGCGCTACTCCAGAGCCTGGAG
CAGGGGCATTTGGGAGGCGCAGCCCTTGATGTATTCGAGTGTGAGAGGTC
CCCTCTTCAGGATCATATCGTCAATCTGTGCAGACTGCCGAATGTGCTGT
GCACTCCGCATATCGGAGGCTCCACGGAGGAAGCCGGAGAACGCGTTTTG
GCTGAAGTATGCGGTGCTGTAGTCGATTTCTTCCAAGGAACTCTGCCGGC
ATCGAAAATTGTCAACGGGGCCCCCGACATGACTGTGCGCTTAAAAAGGC
TTGGCAGCTCATCGTAGCACGCGACAGTCACTACGTATTCGTAAAAAGAA
TGGATTCTGCGCCAGCGCTTGTATGCCGAGATTGTGGGGAAAGGGTGGGT
GTTCGTCCGAAGCCTTCATGGCATATTCGATCTGTTGGTTTGGTCTGGCC
ACGAAGCCTACCTCTATGATGTTCTAAACACGAAGTTTCTTGCCTTTGTG
CCTATTTGTGCTGCCCGGTGTTGCTGATTTTCTTACACCCCCGGTTGCTC
ATGTCCTCTGCTTCCGTCACGTACTCACTGTATTAAGACTGTTTGTGTAT
GCCTCTGATCGTTGATTCACACCCGAGTTTTCCGTGTCATCGTGCGACGT
GGTCGCGTGCGCGTGCGGTTGCGTATGTGTGTGGTGTGCCCTGGCCCAGA
CAAACCCAGTTGGGGAGAGGTGCCTAAGCACACGCACCCCCAAAGACAAG
AAAACGCAAAACAGTAGCAGGGGGTAGGGGGAGGGGTACGCAAAGGTGGT
GAG
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protein sequence of EsuBft1180_3a-0001

>EsuBft1180_3 ID=EsuBft1180_3|Name=EsuBft1180_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=387bp
MRSTGVALAFLAVRSRLTWLGPRQGIYGALRATDTTRRHILVLDPASPAY
QGIETAGYQVTAAHGLSDPELEQVLPAYDAVVVRSANQITRNMLVASPRV
AIVGRAGSGVDNIDLKAASELGVPVVNAPTGNSGSVAELVMGLMLGVSRS
ISPAHDTTRRGEWAKSNFTGRTLSGKTLGIVGFGSVGIALARLAVAFGMV
VMIAEGSSPTPCSGRAEEIDPRASEREGMASQAGVTLPPLPLDELLSKSD
FFSVHCPLTADTKGLISRRELALMKPSAVVINAARGGVVDELALLQSLEQ
GHLGGAALDVFECERSPLQDHIVNLCRLPNVLCTPHIGGSTEEAGERVLA
EVCGAVVDFFQGTLPASKIVNGAPDMTVRLKRLGSSS
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mRNA from alignment at Scaffold_106:178870..183758+

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>EsuBft1180_3a-0001 ID=EsuBft1180_3a-0001|Name=EsuBft1180_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=4889bp|location=Sequence derived from alignment at Scaffold_106:178870..183758+ (Ectocarpus subulatus male Bft15b)
TGCATCTACCACTGTAAAACGATAGAGCGTTGTTTGCATTGCAGCTGCAA
CCGATGCGATCCACAGGCGTAGCCCTCGCGTTCCTCGCGGTACGCTCCAG
GCTTACATGGCTCGGTCCACGTCAGGGTATATACGGAGCTCTTCGTGCAA
CAGACACGACACGAAGACACATTTTGGTCCTAGACCCGGCGTCCCCTGCA
TACCAGGTACACGATATTGTTGCCCCACCACGCAACCACGGGCGCCTCAA
GTTTGTTTGTTTGAGCCGGATTGGTCTTGATCTAGTGCCATAGCTTCTGG
GATCAACCTCTGTTGCGATATTGCAGTAGACTCGATAGATTCCTAAAATG
CGGTGTTCAATTCCTTCATACCATGGCATCTGGACTCGGCTGCAGGAACG
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
AGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
AGGCGGCTGTGTCTATGCTTACGTCCCACACCCTTGAATGCAGGAATGCA
GGAGAGAGATAGCGGGTGGTTTTATCTTTTGTGCTTGCGCACCCTTGCAT
GCCTAAGAGCACCAAGGCTGCTTCCACATTCCATTGGCCACACGATCACA
CAATCTAACTCATCTTTGGCAACCTATGTTCTCGCTTTGTTGTCGTAATC
TGGGCAGGGGATTGAGACAGCTGGATACCAGGTGGGTTTGACAATGTTCA
CCGTAGTCTGCTAAGAGTTGCTCCCCTTTCGTCGAGGCGCACCCCCGCAC
ACACAACAGCACGAAATGATGGGCAGCGCTGACTACAGGGGTTACAATCC
TGGGGTTAATTACGAGGTCAAACAGCGCAAACGAGTTGAACCCCGGCTGT
ACGTACTTTAGTTAAGATGTGCATCATATCCACTCTGCGGCAAGTCAGCG
CCGACTTGCAGCGTAGTGCTTATGCTCTACACCTTAACATGTAGAGATTG
TTCAGAAAGAATTTCGCCTGCACGAGGTAACCCCAGGCGAACATGTTTGT
AGAAAGTAAACATAACCTAACCGAAAGACTATACCCGTTCGAGATATCTA
TAACCTCAGTCGAAAGGGATGTGAAGCAGAAAGTGGATTGAAACCGATTG
GAGACTCTGCTCATTTTTGAGTTTGTGTAAGCCTAACACTGAGTTATCAG
TCTGTATCAGTGATGCCAGTTGGAGCTAAGAACAAAAACATCTGTATAGG
CGGATACATTTTTAATGTAGCCTTCACAGGGAACATGGCGCAGTGTCGTT
TGACAACTTGCTGTTTTTAGAAAGTACCTCTCTACTGTTTTAGGCGACTT
GAATAGACTACAGTATGTACAGCAGTAGGACTGTTGCACACCCACCACAA
AAAAAAAAAAAGTTGGAGTTGTATTGTAGAATTGACTGTATGTAGCTGTT
TCCCGCTTATTTTGAAATCTACGATGTTATGTTGTTCTTCGTGTGTGATG
AGTGTAGGTGAAACGCGTTGAAGGGGGTTTTATAAGTACCATATTTGTTG
CCCATGGATGTTGCTTTCAAGTCACATACCGTGGCACTCCTCAAAATTTG
GAGGGCATGAACTGCCGTGAGCCCGCAAGCGACCCCGACTGCGTTGTCTG
CCGTTCCTAGGTTGCCTCTGCCCAACATCGCGGGTGAAACAGAGATAGAG
TAGCTATGTGCGTGTGGGTGGACAGAGCGGTGTTCTAGACATATAGGCCT
ACCCTCCCGGGTTCTTCTCCTATGCTTGTTTTTGCGATGACGAAACCAAG
TATGAACCCTGGTGCACTTTGCATCATGTTTGATGACCCCTAAAGTTCGC
ACATGACACAGCCTGTGATGTCGCATATTGAGTGTGGCATACATTTCTCG
AACTTGACACGTAAAATCCGAGTAGGTTTCTGGCTGGATGGTACAGCAGC
TGGACGACCACAGAACTCATTGTGTACGGTTTTCCGTGTTCAGCAATCGG
CTGTGAAGCGTTCTCTCTGTTGATTCCACTGCCTGTTTTTCACGTTGTAA
CGTGTGTCTTCGCCTGGTCGTAAGATTTGCCGGCCAGTAACTTGATGGCC
GAAGTTGAACATTGACTGCACCATGGACCAATTATTGAGACTTTGCTGCC
GTTTGCATGACCAATGTCATGGCGCCGTTAACCCTCTTTGTCATCAAGGT
GACCGCCGCGCATGGTTTAAGCGATCCCGAGCTTGAGCAAGTCCTCCCAG
CGTATGACGCGGTCGTGGTTCGTTCGGCCAATCAAATCACTCGCAACATG
CTAGTCGCGAGTCCCCGGGTGGCGATAGTAGGGCGTGCGGGGTCTGGGGT
GGACAATATCGACCTGAAGGCGGCCTCCGAGCTCGGGGTACCTGTTGTGA
ATGCTCCCACGGGAAACTCAGGGTCTGTTGCAGGTCAGCCGACTCGAGTG
CAGCACGGAAAGCGGCTTGCTGATCGCTACTTCCTAGAAAAAATAGCTCC
GGTGCTTTGGGTTGCATCTCGTAGATATAACCGGCAATACGTAGGCAGAT
GAACCGTGAATTTGCTGTTGCGCGATTCTGAGTCTCGAGCAAGCAAGACC
TAGAATATGAATATCAATGATATGATGCATTTAAACGCACCAATCACGCG
TTCTCCGTTGATCTTGTCTCCGCAAGAGTTGGTAATGGGCTTGATGCTCG
GCGTGTCACGGAGCATTTCGCCAGCGCACGACACCACAAGACGCGGGGAA
TGGGCTAAGAGCAATTTCACTGGCCGCACACTCAGCGGAAAGACACTGGG
AATTGTAGGCTTCGGTTCTGTGGGCATAGCGGTACGTGCATACCTATTGC
TCCATTTTTCTTGCTCTCTCGTCGAGTCCCGCCAGAAATCATAGCCTAAA
ACAGATATCCTGCCCCCGCTGGGTTTCCAGCTCTACCTCATTGTTGGCTG
TTGTCGAAGTTCAGGATATGAGGTCAGGCTCATACCCCTCACTTTTTTTT
TCTTGCAGAGTTTCGACATGGTCGACGTCAGGGTTCGAAAACGCGACGAT
CGATCAAAATCGCGTGCATCAAGCGGTCGCAGCATCTTGCAAATGGCTGT
TTTTTGTTGCTTTCCGCTTCGAGGTTGGTATCATGTAGCACCACACGCTA
GCTTGCCAATTTTCGTCTGCTGCCCCGGCCGGGACTGCCGGCACCATGTT
AGTGCTGAAGTCCAGACAAGGGTCGTGTTTGTCTCCGTAGGTCAGCCTCT
CTAACGTACGCCGTGCGTGACGTCGAAAAGAAAATGTCAACTTCCACCGA
CCCTCAACGTCCCTGCAACACATCAGCTGGCAAGACTGGCCGTGGCTTTC
GGCATGGTGGTAATGATAGCAGAAGGGTCGAGTCCGACGCCTTGCTCGGG
GAGAGCTGAGGAAATCGACCCACGCGCATCCGAGAGGGAGGGTATGGCAA
GCCAAGCAGGGGTAACGTTGCCTCCTCTGCCGCTCGACGAACTCTTGTCG
AAGAGCGACTTTTTTTCTGTGCACTGCCCGCTGACGGCGGACACGAAGGG
CCTGATTTCTAGACGCGAACTGGCCCTGATGAAGCCATCTGCCGTGGTTA
TCAATGCGGCGAGAGGAGGAGTGGTGGACGAATTAGGTGCGCGAGTGCTG
TCTCTGTGGGATGCATTGATTGTGTAATTTTTATCAAAGGCCTTCGGATA
GGGCACCCAGATAGCGATGTTCTGTACCTATGTTTCCAACCGGCGTGCTT
AATATTGTCGCGTTAATTGCTCCCAGCGCTACTCCAGAGCCTGGAGCAGG
GGCATTTGGGAGGCGCAGCCCTTGATGTATTCGAGTGTGAGAGGTCCCCT
CTTCAGGATCATATCGTCAATCTGTGCAGGTGACTCTAATCGTGTTTCTT
GCCGTATGTTCGCCTACTTCCTCCCCGCGAATGTGAAATGGACAAAATAG
TCTGCGGCCTTTCCGGCTAACCAGATGAAAGGGCGTGCTTCCGGTAATGG
TTCACAGTGAGCACCTTTGTGGTTGCTGTAGAATACCCGCCTCTTCGCAC
CCTTGATCGCGGCCGATGCAATGTGCCAATCAGACGCTTTTCCCACAGCC
GTCCTAGTTCCTCGTGCACTTCCGATACTGACTGGGTTGAGTTCGACGCC
CACGTGCTGCCGTTGGCAGACTGCCGAATGTGCTGTGCACTCCGCATATC
GGAGGCTCCACGGAGGAAGCCGGAGAACGCGTTTTGGCTGAAGTATGCGG
TGCTGTAGTCGATTTCTTCCAAGGAACTCTGCCGGCATCGAAAATTGTCA
ACGGGGCCCCCGACATGACTGTGCGCTTAAAAAGGCTTGGCAGCTCATCG
TAGCACGCGACAGTCACTACGTATTCGTAAAAAGAATGGATTCTGCGCCA
GCGCTTGTATGCCGAGATTGTGGGGAAAGGGTGGGTGTTCGTCCGAAGCC
TTCATGGCATATTCGATCTGTTGGTTTGGTCTGGCCACGAAGCCTACCTC
TATGATGTTCTAAACACGAAGTTTCTTGCCTTTGTGCCTATTTGTGCTGC
CCGGTGTTGCTGATTTTCTTACACCCCCGGTTGCTCATGTCCTCTGCTTC
CGTCACGTACTCACTGTATTAAGACTGTTTGTGTATGCCTCTGATCGTTG
ATTCACACCCGAGTTTTCCGTGTCATCGTGCGACGTGGTCGCGTGCGCGT
GCGGTTGCGTATGTGTGTGGTGTGCCCTGGCCCAGACAAACCCAGTTGGG
GAGAGGTGCCTAAGCACACGCACCCCCAAAGACAAGAAAACGCAAAACAG
TAGCAGGGGGTAGGGGGAGGGGTACGCAAAGGTGGTGAG
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Coding sequence (CDS) from alignment at Scaffold_106:178870..183758+

>EsuBft1180_3a-0001 ID=EsuBft1180_3a-0001|Name=EsuBft1180_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=CDS|length=1164bp|location=Sequence derived from alignment at Scaffold_106:178870..183758+ (Ectocarpus subulatus male Bft15b)
ATGCGATCCACAGGCGTAGCCCTCGCGTTCCTCGCGGTACGCTCCAGGCT
TACATGGCTCGGTCCACGTCAGGGTATATACGGAGCTCTTCGTGCAACAG
ACACGACACGAAGACACATTTTGGTCCTAGACCCGGCGTCCCCTGCATAC
CAGGGGATTGAGACAGCTGGATACCAGGTGACCGCCGCGCATGGTTTAAG
CGATCCCGAGCTTGAGCAAGTCCTCCCAGCGTATGACGCGGTCGTGGTTC
GTTCGGCCAATCAAATCACTCGCAACATGCTAGTCGCGAGTCCCCGGGTG
GCGATAGTAGGGCGTGCGGGGTCTGGGGTGGACAATATCGACCTGAAGGC
GGCCTCCGAGCTCGGGGTACCTGTTGTGAATGCTCCCACGGGAAACTCAG
GGTCTGTTGCAGAGTTGGTAATGGGCTTGATGCTCGGCGTGTCACGGAGC
ATTTCGCCAGCGCACGACACCACAAGACGCGGGGAATGGGCTAAGAGCAA
TTTCACTGGCCGCACACTCAGCGGAAAGACACTGGGAATTGTAGGCTTCG
GTTCTGTGGGCATAGCGCTGGCAAGACTGGCCGTGGCTTTCGGCATGGTG
GTAATGATAGCAGAAGGGTCGAGTCCGACGCCTTGCTCGGGGAGAGCTGA
GGAAATCGACCCACGCGCATCCGAGAGGGAGGGTATGGCAAGCCAAGCAG
GGGTAACGTTGCCTCCTCTGCCGCTCGACGAACTCTTGTCGAAGAGCGAC
TTTTTTTCTGTGCACTGCCCGCTGACGGCGGACACGAAGGGCCTGATTTC
TAGACGCGAACTGGCCCTGATGAAGCCATCTGCCGTGGTTATCAATGCGG
CGAGAGGAGGAGTGGTGGACGAATTAGCGCTACTCCAGAGCCTGGAGCAG
GGGCATTTGGGAGGCGCAGCCCTTGATGTATTCGAGTGTGAGAGGTCCCC
TCTTCAGGATCATATCGTCAATCTGTGCAGACTGCCGAATGTGCTGTGCA
CTCCGCATATCGGAGGCTCCACGGAGGAAGCCGGAGAACGCGTTTTGGCT
GAAGTATGCGGTGCTGTAGTCGATTTCTTCCAAGGAACTCTGCCGGCATC
GAAAATTGTCAACGGGGCCCCCGACATGACTGTGCGCTTAAAAAGGCTTG
GCAGCTCATCGTAG
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