mRNA_Ecto-sp13_S_contig169.4931.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig169.4931.1
Unique NamemRNA_Ecto-sp13_S_contig169.4931.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: D7FNU5_ECTSI (Elongin-C n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FNU5_ECTSI)

HSP 1 Score: 191 bits (486), Expect = 9.140e-62
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 1 
Query:    1 QEFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            QEFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC
Sbjct:    6 QEFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 100
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A6U1KLA4_9STRA (Elongin-C n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A6U1KLA4_9STRA)

HSP 1 Score: 138 bits (347), Expect = 2.500e-40
Identity = 69/94 (73.40%), Postives = 79/94 (84.04%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            ++VKL+SAEG EF+VDR+CA  SGTIKAMLSG F E+ SG++ F EISTPILEKVIQ+ YYK  Y NS  RIPEF IEPE+ALELLMAANFLDC
Sbjct:   26 QYVKLISAEGHEFFVDRQCAQTSGTIKAMLSGSFTES-SGEIKFPEISTPILEKVIQYFYYKASYKNSQVRIPEFAIEPEVALELLMAANFLDC 118
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A1V9Z8H2_9STRA (Elongin-C n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A1V9Z8H2_9STRA)

HSP 1 Score: 137 bits (344), Expect = 4.270e-40
Identity = 68/94 (72.34%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            E+VKL+SAEG EF+V RKCA+VSGTIKAMLSG F E+  G+V F +IS PILEKVIQ++YYK RY+NS  RIP+F IEPE+ALELLMAAN+LDC
Sbjct:    9 EYVKLISAEGHEFFVARKCALVSGTIKAMLSGHFSES-KGEVRFPDISAPILEKVIQYMYYKKRYSNSNARIPDFGIEPEMALELLMAANYLDC 101
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A6G0WB49_9STRA (Elongin-C n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WB49_9STRA)

HSP 1 Score: 135 bits (340), Expect = 1.850e-39
Identity = 65/94 (69.15%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            ++VKL+SAEG EF+V RKCAMVSGTIKAML+G F E+  G+V F +IS PILEK+IQ+ Y+KVRY+NS  R+P+F IEPE+ALELLMAAN+LDC
Sbjct:   11 DYVKLISAEGQEFYVARKCAMVSGTIKAMLTGHFSES-KGEVRFPDISAPILEKIIQYFYFKVRYSNSNARLPDFTIEPEIALELLMAANYLDC 103
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A7S2XYA0_9STRA (Elongin-C n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XYA0_9STRA)

HSP 1 Score: 135 bits (339), Expect = 2.780e-39
Identity = 68/92 (73.91%), Postives = 79/92 (85.87%), Query Frame = 1 
Query:   10 VKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            VKLVS+EG EF+ DR+CAMVSGTI AMLSG F E+  G+V F EIS+ ILEK+IQ+ YYKVRYTNS +RIP+F IEPE+ALELLMAANFLDC
Sbjct:   15 VKLVSSEGHEFYCDRRCAMVSGTINAMLSGQFAESR-GEVTFPEISSAILEKIIQYCYYKVRYTNSTSRIPDFPIEPEIALELLMAANFLDC 105
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A1W0A8X8_9STRA (Elongin-C n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A8X8_9STRA)

HSP 1 Score: 134 bits (338), Expect = 3.950e-39
Identity = 66/94 (70.21%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            E+VKLVSAEG EF+V RKCA+VSGTIKAML G F E+  G++ F +IS PILEKVIQ++YYK RY+NS  RIPEF+IEPE++LELLMAAN+L+C
Sbjct:   13 EYVKLVSAEGHEFYVARKCALVSGTIKAMLLGQFSES-KGEIRFPDISAPILEKVIQYLYYKKRYSNSNARIPEFNIEPEMSLELLMAANYLEC 105
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: L8GYQ9_ACACA (Elongin-C n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GYQ9_ACACA)

HSP 1 Score: 134 bits (337), Expect = 5.290e-39
Identity = 70/93 (75.27%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:   10 VKLVSAEGAEFWVDRKCAMVSGTIKAMLSGG--FGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLD 282
            VKL+SA+G EF VDRK AMVSGTIK+MLSG   F E A G+++F EISTPILEKVIQ+ YYK+RYTNS T IPEF IEPE+ALELLMAANFLD
Sbjct:   10 VKLISADGHEFVVDRKAAMVSGTIKSMLSGPGTFTEQAMGEINFREISTPILEKVIQYFYYKLRYTNSTTEIPEFPIEPEVALELLMAANFLD 102
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: F0YER3_AURAN (Elongin-C (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YER3_AURAN)

HSP 1 Score: 133 bits (335), Expect = 7.670e-39
Identity = 67/92 (72.83%), Postives = 75/92 (81.52%), Query Frame = 1 
Query:   22 SAEGAEFWVDRKCAMVSGTIKAMLSGGFGEA----ASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            SAEG EFWVDRKCA  SGTIKAMLSG  G      +SG++ F EISTPILEKVIQ+ YYK+RYTNS  RIPEF I+PE+ALELLMA+NFLDC
Sbjct:    1 SAEGHEFWVDRKCANTSGTIKAMLSGPRGPRQFTESSGEIKFPEISTPILEKVIQYFYYKLRYTNSQVRIPEFQIDPEMALELLMASNFLDC 92
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: K8Z122_NANGC (Elongin-C n=2 Tax=Monodopsidaceae TaxID=425072 RepID=K8Z122_NANGC)

HSP 1 Score: 133 bits (334), Expect = 1.340e-38
Identity = 65/94 (69.15%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            ++VKL+SAEG EF VDRKCA VS TI+AML+G F E+  G+++F EIST ILEKVIQ++YYKVRYTNS  ++P+F +EPE+ALELLMAANFLDC
Sbjct:    7 KYVKLISAEGHEFIVDRKCACVSKTIEAMLAGNFAES-KGEINFPEISTNILEKVIQYLYYKVRYTNSPQKVPDFKMEPEIALELLMAANFLDC 99
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BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Match: A0A485K8T6_9STRA (Elongin-C n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485K8T6_9STRA)

HSP 1 Score: 131 bits (330), Expect = 6.540e-38
Identity = 63/94 (67.02%), Postives = 79/94 (84.04%), Query Frame = 1 
Query:    4 EFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEISTPILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC 285
            E+VKL+SAEG EF++ RKCAMVSGTIKAML+G F E+  G++ F +I   +LEKVIQ++YYK RY+NS  RIP+F IEPE+ALELLMAAN+LDC
Sbjct:   13 EYVKLISAEGHEFYIARKCAMVSGTIKAMLTGHFSES-KGEIRFPDIGASVLEKVIQYMYYKKRYSNSNARIPDFAIEPEIALELLMAANYLDC 105
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig169.4931.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FNU5_ECTSI9.140e-62100.00Elongin-C n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FN... [more]
A0A6U1KLA4_9STRA2.500e-4073.40Elongin-C n=1 Tax=Pelagomonas calceolata TaxID=356... [more]
A0A1V9Z8H2_9STRA4.270e-4072.34Elongin-C n=3 Tax=Saprolegniaceae TaxID=4764 RepID... [more]
A0A6G0WB49_9STRA1.850e-3969.15Elongin-C n=1 Tax=Aphanomyces euteiches TaxID=1008... [more]
A0A7S2XYA0_9STRA2.780e-3973.91Elongin-C n=1 Tax=Fibrocapsa japonica TaxID=94617 ... [more]
A0A1W0A8X8_9STRA3.950e-3970.21Elongin-C n=1 Tax=Thraustotheca clavata TaxID=7455... [more]
L8GYQ9_ACACA5.290e-3975.27Elongin-C n=1 Tax=Acanthamoeba castellanii str. Ne... [more]
F0YER3_AURAN7.670e-3972.83Elongin-C (Fragment) n=1 Tax=Aureococcus anophagef... [more]
K8Z122_NANGC1.340e-3869.15Elongin-C n=2 Tax=Monodopsidaceae TaxID=425072 Rep... [more]
A0A485K8T6_9STRA6.540e-3867.02Elongin-C n=1 Tax=Aphanomyces stellatus TaxID=1203... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig169contigEcto-sp13_S_contig169:17462..18569 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score191.0
Seed ortholog evalue2.5e-46
Seed eggNOG ortholog2880.D7FNU5
Preferred nameTCEB1
Model size288
KEGG koko:K03872
KEGG Pathwayko04066,ko04120,ko05200,ko05211,map04066,map04120,map05200,map05211
KEGG ModuleM00383,M00388
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000151,GO:0000153,GO:0000280,GO:0000578,GO:0000819,GO:0001666,GO:0002009,GO:0002165,GO:0003002,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005694,GO:0005700,GO:0005705,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006281,GO:0006289,GO:0006351,GO:0006354,GO:0006355,GO:0006357,GO:0006366,GO:0006368,GO:0006464,GO:0006725,GO:0006807,GO:0006928,GO:0006950,GO:0006974,GO:0006996,GO:0007017,GO:0007018,GO:0007049,GO:0007059,GO:0007135,GO:0007275,GO:0007350,GO:0007351,GO:0007389,GO:0007444,GO:0007472,GO:0007474,GO:0007476,GO:0007552,GO:0007560,GO:0008023,GO:0008150,GO:0008152,GO:0008595,GO:0009058,GO:0009059,GO:0009628,GO:0009653,GO:0009790,GO:0009791,GO:0009798,GO:0009880,GO:0009886,GO:0009887,GO:0009888,GO:0009889,GO:0009891,GO:0009893,GO:0009948,GO:0009952,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010564,GO:0010604,GO:0010628,GO:0010638,GO:0016043,GO:0016070,GO:0016344,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019538,GO:0019904,GO:0022402,GO:0022414,GO:0030891,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031461,GO:0031462,GO:0031463,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0032774,GO:0032784,GO:0032786,GO:0032968,GO:0032991,GO:0033043,GO:0033044,GO:0033045,GO:0033554,GO:0034243,GO:0034641,GO:0034645,GO:0034654,GO:0035107,GO:0035114,GO:0035120,GO:0035220,GO:0035239,GO:0035282,GO:0035295,GO:0036211,GO:0036293,GO:0036294,GO:0040020,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0043618,GO:0043620,GO:0043687,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0044877,GO:0045132,GO:0045144,GO:0045787,GO:0045836,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0048285,GO:0048513,GO:0048518,GO:0048522,GO:0048563,GO:0048569,GO:0048707,GO:0048729,GO:0048731,GO:0048736,GO:0048737,GO:0048856,GO:0050000,GO:0050789,GO:0050794,GO:0050896,GO:0051128,GO:0051130,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051252,GO:0051254,GO:0051276,GO:0051303,GO:0051305,GO:0051321,GO:0051445,GO:0051446,GO:0051640,GO:0051641,GO:0051649,GO:0051656,GO:0051716,GO:0051726,GO:0051759,GO:0051783,GO:0051785,GO:0051983,GO:0051984,GO:0060255,GO:0060429,GO:0060562,GO:0061418,GO:0061983,GO:0065007,GO:0070013,GO:0070192,GO:0070449,GO:0070482,GO:0070887,GO:0070911,GO:0071453,GO:0071456,GO:0071704,GO:0071840,GO:0080090,GO:0090068,GO:0090304,GO:0097659,GO:0098687,GO:0098813,GO:0140013,GO:1901360,GO:1901362,GO:1901564,GO:1901576,GO:1901987,GO:1901989,GO:1901993,GO:1901995,GO:1902099,GO:1902101,GO:1902102,GO:1902104,GO:1902494,GO:1902680,GO:1903046,GO:1903506,GO:1903508,GO:1905132,GO:1905134,GO:1905818,GO:1905820,GO:1990234,GO:2000112,GO:2000241,GO:2000243,GO:2001141
Exons3
EggNOG free text desc.positive regulation of transcription elongation from RNA polymerase II promoter
EggNOG OGsKOG3473@1,KOG3473@2759
Ec32 ortholog descriptionRNA polymerase II transcription elongation factor Elongin/SIII, subunit elongin C
Ec32 orthologEc-12_003330.1
Cds size288
COG Functional cat.I
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03400,ko04121
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462885.4051142-CDS-Ecto-sp13_S_contig169:17461..175581681462885.4051142-CDS-Ecto-sp13_S_contig169:17461..17558Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig169 17462..17558 +
1681462885.4182777-CDS-Ecto-sp13_S_contig169:17872..179641681462885.4182777-CDS-Ecto-sp13_S_contig169:17872..17964Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig169 17873..17964 +
1681462885.4337347-CDS-Ecto-sp13_S_contig169:18470..185691681462885.4337347-CDS-Ecto-sp13_S_contig169:18470..18569Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig169 18471..18569 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig169.4931.1prot_Ecto-sp13_S_contig169.4931.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig169 17462..18569 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig169.4931.1

>prot_Ecto-sp13_S_contig169.4931.1 ID=prot_Ecto-sp13_S_contig169.4931.1|Name=mRNA_Ecto-sp13_S_contig169.4931.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=96bp
QEFVKLVSAEGAEFWVDRKCAMVSGTIKAMLSGGFGEAASGQVHFAEIST
PILEKVIQFVYYKVRYTNSATRIPEFHIEPELALELLMAANFLDC*
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mRNA from alignment at Ecto-sp13_S_contig169:17462..18569+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig169.4931.1 ID=mRNA_Ecto-sp13_S_contig169.4931.1|Name=mRNA_Ecto-sp13_S_contig169.4931.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1108bp|location=Sequence derived from alignment at Ecto-sp13_S_contig169:17462..18569+ (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGAGTTCGTGAAGCTGGTCAGTGCGGAGGGCGCAGAATTTTGGGTCGA
CCGAAAGTGTGCCATGGTGTCGGGGACCATCAAGGCAATGCTTAGCGGTG
AGAACGAAGAGGGGTGACAGAGTGTTTAGCGCGAGACTCCTGTGGGGCGG
CGGGCAGGGATGGGTCGACCTCCCCGGCAGGATATTCATGCCGTTTCCTT
CGGAGAAAAATGCGTTGAAAACACAAGCACCGAGTCTCCAACAAGGGTTG
GTGGGTATGGCCAATCAATGTCCTTGTATTCGATCCTTTGGCCACCTCCC
TCTTGGTTGGTCTTTTTTTGTGGCTTTTGACCAATGCCCCGGTACTCGGT
TCTTCCCCCGCCTCCTTCTCTCACCTGCACGTTGGTTGATGTCGGTTTGT
GTTGTTGGCAGGGGGGTTCGGAGAGGCGGCATCGGGACAGGTCCACTTCG
CCGAAATCTCGACACCAATATTGGAGAAGGTCATCCAGTTTGTGTACTAC
AAGGTGAGGGAGAGGTGACGGATGTTACATTCTGCAACCATTGCATGTAA
TTGTTTTGGCATTGCTGGGACCACGTGGTGCCCGAAGACTCGAGCGATGC
GCTAATATGTCCATACGTGTGCGTTGACCGTCTGGGGTCACTCAGGCTAG
CGTGCACGGTGAACACCAGCGGTCTGTTAATGCGCCGTGTGTGCAGCTGA
TGTAAATAATCACTCGCGGCACTTTCTTGAGCTTATCTTGTCTAGAACGT
TGCTCCAGTGTGTAAATAGTGCTTATTTTTCTTGTTGTGGGAGTTCATCC
TAACGTACATCGCGTACACGAATGTACTTCACCAAGAGCAACGAACGGTA
CACGTGTTCCCCGTATTTCCTCTGCAGAATACCCATTTTCAACTGTTTTG
AGGTCGTATTTATAAAGCTTTGCTGTTTTGGGGGTAGGTGAAACGAACGA
GTAGCAGCCGGACAACAACACAACCGCGCCATTTCGCCTTGTGCTCTTCG
ATACCCCAGGTGCGATACACCAACAGCGCGACCCGCATCCCAGAGTTTCA
CATCGAGCCGGAGCTGGCTCTGGAGTTGCTCATGGCCGCAAACTTTCTCG
ACTGCTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig169:17462..18569+

>mRNA_Ecto-sp13_S_contig169.4931.1 ID=mRNA_Ecto-sp13_S_contig169.4931.1|Name=mRNA_Ecto-sp13_S_contig169.4931.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=288bp|location=Sequence derived from alignment at Ecto-sp13_S_contig169:17462..18569+ (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGAGTTCGTGAAGCTGGTCAGTGCGGAGGGCGCAGAATTTTGGGTCGA
CCGAAAGTGTGCCATGGTGTCGGGGACCATCAAGGCAATGCTTAGCGGGG
GGTTCGGAGAGGCGGCATCGGGACAGGTCCACTTCGCCGAAATCTCGACA
CCAATATTGGAGAAGGTCATCCAGTTTGTGTACTACAAGGTGCGATACAC
CAACAGCGCGACCCGCATCCCAGAGTTTCACATCGAGCCGGAGCTGGCTC
TGGAGTTGCTCATGGCCGCAAACTTTCTCGACTGCTGA
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