mRNA_Ecto-sp13_S_contig16468.4661.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_Ecto-sp13_S_contig16468.4661.1
Unique Name	mRNA_Ecto-sp13_S_contig16468.4661.1
Type	mRNA
Organism	Ectocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)

Homology

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BLAST of mRNA_Ecto-sp13_S_contig16468.4661.1 vs. uniprot
Match: D7FH97_ECTSI (Similar to chromosome fragility associated gene 1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FH97_ECTSI)

HSP 1 Score: 333 bits (853), Expect = 3.480e-94
Identity = 383/749 (51.13%), Postives = 407/749 (54.34%), Query Frame = 1

Query:   70 VPPLRPSNGQDTPAAAPAVGMKKPIPP--ATGTKRKGKLRLPSAKTKGVSGKGGXXXXXXXGQDAAPAVATLAADXXXXXXXXXXXXXXXXXXXXXXXXGCGADQTPSTESKKTKKK-QASVWEGCGFTGVGEPPSVASAPVDAADDDEDAATGASAAGTVAADIATGSRDVVGAA--DDAADSPAADDDVADGGAKVGPGREETSDLSSPCPVKPSSTGWKRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------GGMPAVSERRVPSDEVPAKQNDDLGGEGSDAAVPGAAXXXXXXXXXXXXXXXXXXXXXXTEPTVAEAVTQEVEPRASDTETGGAGRGRKHGDDVPVAADPWTPESTPVPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGVALXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGAVAPVASNVGTAAVASPLGRVSKRVAARAAEQAKXXXXXXXXXXXXXXXXLEAKARRKEXXXXXXXXXXXXXXXXXXXXXXIISGDFPPVVSGDSLPASHKKGVTIGMTTPTEGVPHGPKTRVILGNIPPAAGTAGKPAKLASIFLPRAGGLGSAAPAAGPKLAPLFMPKRKASPETIGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGRKEASLPSSAAPPSIEV 2283
            VPPLRP+NGQDTPAA              ATGTK+KGKLRLPS +TKG +GKGG       G+ AAPA AT    XXXXXXXXXXXXXXXXXXXXXXXXGCGA   P+TESK      QASVWEGCGFTGVGEP S ASAP DAA DD+DAATGASA+G VAA               DDAA+SPAA+DDVADGGAKVGPG+EETS+LSSPCPV+PSSTG KR XXXXXXX               XXXXXXXXXX        GGMPAVSER V S  VPAKQNDDLG +G DAAVPGAA     XXXXX             EP V EAVT++  PRASDTE       +++G DVPVAADP TPESTPVPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                                                                                                                                                                                                  IISGD P VVSGD LP SHKKG   GMTT  EGVPH PKTRVI GNIPPAAGTAGKPAKLASIFLPRAGG G AAPAAGPKLAPLF+PKRKASPET+     XXXXXXXXXXXXXXXXX         SGRK+ASLPSS +PPSIE+
Sbjct:   70 VPPLRPNNGQDTPAAXXXXXXXXXXXXXXATGTKKKGKLRLPSRRTKGGAGKGG-------GEGAAPAAATSXXXXXXXXXXXXXXXXXXXXXXXXXXXGCGAALAPTTESKXXXXXXQASVWEGCGFTGVGEPLSAASAPADAAGDDKDAATGASASGAVAAXXXXXXXXXXXXXXXDDAAESPAANDDVADGGAKVGPGQEETSELSSPCPVEPSSTGQKRXXXXXXXXHAESARSETPGGCARXXXXXXXXXXPPLEGDDDGGMPAVSERLVLSATVPAKQNDDLGVDGGDAAVPGAAAITAAXXXXXEGAAPLAVAAI--EPAVGEAVTEQAVPRASDTE-------KENGGDVPVAADPGTPESTPVPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IISGDCPTVVSGDPLPTSHKKGAATGMTTSAEGVPHAPKTRVIPGNIPPAAGTAGKPAKLASIFLPRAGGWGGAAPAAGPKLAPLFVPKRKASPETVSDVGGXXXXXXXXXXXXXXXXX---------SGRKKASLPSSVSPPSIEI 599

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BLAST of mRNA_Ecto-sp13_S_contig16468.4661.1 vs. uniprot
Match: A0A6H5KGC2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KGC2_9PHAE)

HSP 1 Score: 182 bits (461), Expect = 6.020e-44
Identity = 153/183 (83.61%), Postives = 156/183 (85.25%), Query Frame = 1

Query: 1759 KARRKEXXXXXXXXXXXXXXXXXXXXXXIISGDFPPVVSGDSLPASHKKGVTIGMTTPTEGVPHGPKTRVILGNIPPAAGTAGKPAKLASIFLPRAGGLGSAAPAAGPKLAPLFMPKRKASPETIGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------SGRKEASLPSSAAPPSIEV 2283
            KARRKEXXXXXXXXXXXXXXXX      IISGD PPVVSGDSLPASHKKGVT GMTT TEGVPHGPKTRVI GNIPPAAGTAG PAKLASIFLPRAGG G AAPAAGPKLAPLFMPKRK SPET  G XXXXXXXXXXXXXXXXXXXXXXXXXXXX        SGRK+ASLPS+  PPSIE+
Sbjct:  161 KARRKEXXXXXXXXXXXXXXXXVAATTTIISGDSPPVVSGDSLPASHKKGVTTGMTTSTEGVPHGPKTRVIPGNIPPAAGTAGNPAKLASIFLPRAGGWGGAAPAAGPKLAPLFMPKRKPSPETFSGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGRKKASLPSAVTPPSIEI 343

The following BLAST results are available for this feature:

BLAST of mRNA_Ecto-sp13_S_contig16468.4661.1 vs. uniprot

BLAST of mRNA_Ecto-sp13_S_contig16468.4661.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2


Match Name	E-value	Identity	Description
D7FH97_ECTSI	3.480e-94	51.13	Similar to chromosome fragility associated gene 1 ...	[more]
A0A6H5KGC2_9PHAE	6.020e-44	83.61	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Ecto-sp13_S_contig16468	contig	Ecto-sp13_S_contig16468:222..3073 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.0	2022-07-08

Properties


Property Name	Value
Taxonomic scope	Eukaryota
Stop	1
Start	0
Seed ortholog score	341.7
Seed ortholog evalue	9.1e-91
Seed eggNOG ortholog	2880.D7FH97
Model size	2289
KEGG ko	ko:K10754
KEGG Pathway	ko03030,ko03420,ko03430,map03030,map03420,map03430
KEGG Module	M00289,M00295
Hectar predicted targeting category	other localisation
Exons	2
EggNOG free text desc.	DNA clamp loader activity
EggNOG OGs	COG5275@1,KOG1968@2759
Cds size	2289
COG Functional cat.	S
Best tax level	Eukaryota
Best eggNOG OG	NA\|NA\|NA
BRITE	ko00000,ko00001,ko00002,ko03032,ko03400

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681462865.7950594-CDS-Ecto-sp13_S_contig16468:221..604	1681462865.7950594-CDS-Ecto-sp13_S_contig16468:221..604	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig16468 222..604 -
1681462865.8122144-CDS-Ecto-sp13_S_contig16468:1167..3073	1681462865.8122144-CDS-Ecto-sp13_S_contig16468:1167..3073	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig16468 1168..3073 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_Ecto-sp13_S_contig16468.4661.1	prot_Ecto-sp13_S_contig16468.4661.1	Ectocarpus species13 EcNAP12_S_4_19m	polypeptide	Ecto-sp13_S_contig16468 222..3073 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at Ecto-sp13_S_contig16468:222..3073-
coding sequence from alignment at Ecto-sp13_S_contig16468:222..3073-

protein sequence of mRNA_Ecto-sp13_S_contig16468.4661.1

>prot_Ecto-sp13_S_contig16468.4661.1 ID=prot_Ecto-sp13_S_contig16468.4661.1|Name=mRNA_Ecto-sp13_S_contig16468.4661.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=763bp
GSGTGPAAAPPTPGITGTAGSSGVPPLRPSNGQDTPAAAPAVGMKKPIPP
ATGTKRKGKLRLPSAKTKGVSGKGGGGAGKGGGQDAAPAVATLAADAAAA
KSPKSATVRSPKARAARSPKGCGADQTPSTESKKTKKKQASVWEGCGFTG
VGEPPSVASAPVDAADDDEDAATGASAAGTVAADIATGSRDVVGAADDAA
DSPAADDDVADGGAKVGPGREETSDLSSPCPVKPSSTGWKRRAAGGGGGH
ADSARSETPGEGAAAAAATTTEDDNDGGMPAVSERRVPSDEVPAKQNDDL
GGEGSDAAVPGAAAVAVAAAATAAAEGAAPPAVAATEPTVAEAVTQEVEP
RASDTETGGAGRGRKHGDDVPVAADPWTPESTPVPPPPLAAAADGDDPEI
TTTAVAGDGSVAGAATAGDSVVSDAGVALAAAAAAAAAAAAAAAAAGSSK
ACSPGSGGSDGGVPGGGGAGAAPPAAGAAPPAARGGGAAAPPATAAAPPP
AAAAAGACAGSGAAPPAAVDDTVAASSGASAAGAVAPVASNVGTAAVASP
LGRVSKRVAARAAEQAKAAAAAAEAEAELAAAALEAKARRKEAAAAAKKS
KKKASKAAAAATTTIISGDFPPVVSGDSLPASHKKGVTIGMTTPTEGVPH
GPKTRVILGNIPPAAGTAGKPAKLASIFLPRAGGLGSAAPAAGPKLAPLF
MPKRKASPETIGGGSGGGGGGGGGGGGRRSGGGGEKEKEKEKSGRKEASL
PSSAAPPSIEVR*

mRNA from alignment at Ecto-sp13_S_contig16468:222..3073-

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_Ecto-sp13_S_contig16468.4661.1 ID=mRNA_Ecto-sp13_S_contig16468.4661.1|Name=mRNA_Ecto-sp13_S_contig16468.4661.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2852bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16468:222..3073- (Ectocarpus species13 EcNAP12_S_4_19m)
GGAAGCGGGACAGGGCCAGCGGCAGCACCACCAACACCCGGCATCACCGG
CACCGCCGGCAGCAGCGGCGTCCCCCCCCTCCGGCCTAGCAACGGGCAGG
ACACCCCCGCCGCCGCCCCCGCCGTTGGCATGAAAAAGCCTATACCACCA
GCAACAGGCACCAAGAGAAAGGGAAAGCTCCGCCTACCGTCCGCGAAAAC
GAAGGGCGTCTCCGGTAAGGGCGGGGGCGGTGCCGGGAAGGGCGGGGGTC
AGGACGCCGCGCCAGCGGTGGCGACCTTAGCCGCCGATGCAGCAGCAGCG
AAATCTCCCAAATCTGCGACGGTGAGATCTCCGAAGGCTAGGGCGGCGAG
ATCTCCGAAAGGGTGCGGCGCGGACCAGACCCCCTCTACGGAGAGCAAGA
AGACGAAGAAGAAGCAGGCGAGCGTGTGGGAAGGGTGTGGGTTTACCGGC
GTGGGGGAACCTCCCAGCGTTGCATCGGCTCCCGTCGACGCTGCTGATGA
TGATGAGGACGCCGCTACGGGCGCTTCCGCTGCGGGTACCGTGGCTGCTG
ATATCGCTACGGGTTCTCGCGATGTGGTAGGTGCTGCTGATGATGCTGCG
GATTCTCCCGCTGCGGATGATGATGTTGCTGATGGGGGCGCTAAGGTTGG
CCCAGGACGGGAGGAGACCAGCGACTTATCGTCACCATGCCCGGTGAAGC
CATCGTCAACGGGTTGGAAGAGGCGAGCAGCGGGCGGGGGGGGGGGACAC
GCGGATAGTGCCCGCTCCGAGACACCGGGGGAGGGTGCTGCTGCTGCTGC
TGCTACTACTACCGAGGACGACAATGACGGCGGCATGCCGGCCGTATCGG
AGCGCCGTGTCCCTTCGGACGAAGTGCCCGCAAAGCAGAACGACGACCTC
GGAGGCGAAGGCAGCGATGCTGCCGTGCCGGGCGCAGCAGCGGTAGCGGT
AGCGGCGGCGGCGACGGCGGCGGCGGAAGGAGCGGCACCGCCAGCGGTGG
CAGCGACAGAACCGACGGTAGCGGAGGCGGTGACGCAGGAGGTGGAACCG
AGAGCCTCGGACACGGAGACAGGAGGAGCGGGCCGGGGCCGAAAGCACGG
CGACGACGTGCCCGTCGCGGCGGATCCATGGACACCCGAATCGACACCGG
TTCCGCCGCCTCCTTTGGCGGCCGCTGCAGACGGAGATGACCCTGAGATT
ACTACCACTGCTGTGGCTGGGGATGGGAGTGTCGCCGGTGCCGCCACCGC
CGGTGATTCGGTTGTCAGCGATGCTGGGGTTGCCCTTGCTGCTGCTGCTG
CTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGTAGTAGTAAA
GCTTGTTCTCCTGGAAGCGGCGGCAGTGATGGCGGCGTACCTGGTGGTGG
TGGTGCTGGTGCTGCTCCTCCTGCCGCTGGTGCTGCTCCTCCTGCTGCTC
GTGGTGGTGGTGCTGCTGCTCCCCCTGCTACTGCTGCTGCTCCTCCCCCT
GCTGCTGCTGCTGCTGGTGCTTGTGCTGGTTCTGGTGCTGCTCCTCCTGC
TGCTGTTGATGATACTGTTGCTGCTAGTTCTGGTGCTAGTGCTGCTGGTG
CTGTCGCTCCTGTCGCCAGCAACGTAGGAACGGCGGCGGTGGCGTCTCCC
CTAGGCCGGGTGTCCAAGCGCGTCGCCGCCCGGGCGGCAGAGCAGGCCAA
GGCGGCGGCGGCGGCGGCGGAGGCGGAGGCGGAGCTGGCGGCGGCGGCCC
TGGAAGCGAAGGCGCGCCGCAAAGAGGCCGCCGCCGCGGCTAAGAAGAGC
AAGAAGAAGGCTTCCAAGGCGGCGGCGGCGGCGACGACGACGATCATCTC
CGGAGATTTCCCACCGGTGGTCTCCGGAGATTCCCTCCCAGCGTCACACA
AGAAAGGTATGTTTTGGGCGAACTCGATGCAGATATCCAGAGATACCCCA
ACGGTGGTCTCTGGAGATTCCCTCCCAACGCCACGCAAGAGAGGTGTGTG
TGGGGTGATAGTGCCTGCTACGACATTGGAGGAGGCATGGCAAGCCGAAA
TCACTCTTTTGTATCCGTACTTTTCCTTTTGCCGGTTCTCTTTTGCCGTA
GCCGGCACTGCTGTGTACTCGGCCCTCTCGATACGGATCTAGCGGGTTCC
GGGTATAAGTCATTGGTGGTATTACTATGTTACGCGTTTGTGGAAGGGGG
CAATCCCGCTCCTGCGTGCATTCCTGTTGCTCTGCGTTTCGGACCACCCC
CCGCCACGGGGGGGGAGAATGTACAACAGCCTACCATGGACGAGCAAGCC
CTGCTTTCTCTCTCCAACTCTCTCTCTCTGAACAATACCTCTTGCTCTGC
GCTTCTGACAACATGCCGCCATCGGCATGCATGCCCGGCGTACTGTTTCT
TACCTGCTCTCTCTGAACAATACCTTTTGCTCTGCGCTTCTGACCAACAA
CCTCCCCGCCTACCGTCAGGGGTCACCATCGGTATGACTACTCCCACGGA
AGGCGTTCCGCATGGCCCCAAGACGCGCGTCATATTGGGGAACATACCGC
CGGCTGCTGGAACGGCGGGGAAGCCCGCCAAGCTAGCCTCGATCTTCCTT
CCCAGGGCGGGCGGCTTGGGGAGCGCGGCGCCTGCGGCGGGTCCGAAGCT
GGCGCCGCTCTTCATGCCCAAGAGGAAGGCTAGCCCTGAGACCATTGGCG
GCGGCAGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGTGGTCGTCGT
AGTGGCGGTGGTGGGGAGAAAGAGAAAGAGAAAGAAAAGAGCGGACGGAA
AGAGGCTTCTCTTCCTTCTTCGGCGGCTCCTCCGAGCATCGAGGTGCGCT
AA

Coding sequence (CDS) from alignment at Ecto-sp13_S_contig16468:222..3073-

>mRNA_Ecto-sp13_S_contig16468.4661.1 ID=mRNA_Ecto-sp13_S_contig16468.4661.1|Name=mRNA_Ecto-sp13_S_contig16468.4661.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=2289bp|location=Sequence derived from alignment at Ecto-sp13_S_contig16468:222..3073- (Ectocarpus species13 EcNAP12_S_4_19m)
GGAAGCGGGACAGGGCCAGCGGCAGCACCACCAACACCCGGCATCACCGG
CACCGCCGGCAGCAGCGGCGTCCCCCCCCTCCGGCCTAGCAACGGGCAGG
ACACCCCCGCCGCCGCCCCCGCCGTTGGCATGAAAAAGCCTATACCACCA
GCAACAGGCACCAAGAGAAAGGGAAAGCTCCGCCTACCGTCCGCGAAAAC
GAAGGGCGTCTCCGGTAAGGGCGGGGGCGGTGCCGGGAAGGGCGGGGGTC
AGGACGCCGCGCCAGCGGTGGCGACCTTAGCCGCCGATGCAGCAGCAGCG
AAATCTCCCAAATCTGCGACGGTGAGATCTCCGAAGGCTAGGGCGGCGAG
ATCTCCGAAAGGGTGCGGCGCGGACCAGACCCCCTCTACGGAGAGCAAGA
AGACGAAGAAGAAGCAGGCGAGCGTGTGGGAAGGGTGTGGGTTTACCGGC
GTGGGGGAACCTCCCAGCGTTGCATCGGCTCCCGTCGACGCTGCTGATGA
TGATGAGGACGCCGCTACGGGCGCTTCCGCTGCGGGTACCGTGGCTGCTG
ATATCGCTACGGGTTCTCGCGATGTGGTAGGTGCTGCTGATGATGCTGCG
GATTCTCCCGCTGCGGATGATGATGTTGCTGATGGGGGCGCTAAGGTTGG
CCCAGGACGGGAGGAGACCAGCGACTTATCGTCACCATGCCCGGTGAAGC
CATCGTCAACGGGTTGGAAGAGGCGAGCAGCGGGCGGGGGGGGGGGACAC
GCGGATAGTGCCCGCTCCGAGACACCGGGGGAGGGTGCTGCTGCTGCTGC
TGCTACTACTACCGAGGACGACAATGACGGCGGCATGCCGGCCGTATCGG
AGCGCCGTGTCCCTTCGGACGAAGTGCCCGCAAAGCAGAACGACGACCTC
GGAGGCGAAGGCAGCGATGCTGCCGTGCCGGGCGCAGCAGCGGTAGCGGT
AGCGGCGGCGGCGACGGCGGCGGCGGAAGGAGCGGCACCGCCAGCGGTGG
CAGCGACAGAACCGACGGTAGCGGAGGCGGTGACGCAGGAGGTGGAACCG
AGAGCCTCGGACACGGAGACAGGAGGAGCGGGCCGGGGCCGAAAGCACGG
CGACGACGTGCCCGTCGCGGCGGATCCATGGACACCCGAATCGACACCGG
TTCCGCCGCCTCCTTTGGCGGCCGCTGCAGACGGAGATGACCCTGAGATT
ACTACCACTGCTGTGGCTGGGGATGGGAGTGTCGCCGGTGCCGCCACCGC
CGGTGATTCGGTTGTCAGCGATGCTGGGGTTGCCCTTGCTGCTGCTGCTG
CTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGTAGTAGTAAA
GCTTGTTCTCCTGGAAGCGGCGGCAGTGATGGCGGCGTACCTGGTGGTGG
TGGTGCTGGTGCTGCTCCTCCTGCCGCTGGTGCTGCTCCTCCTGCTGCTC
GTGGTGGTGGTGCTGCTGCTCCCCCTGCTACTGCTGCTGCTCCTCCCCCT
GCTGCTGCTGCTGCTGGTGCTTGTGCTGGTTCTGGTGCTGCTCCTCCTGC
TGCTGTTGATGATACTGTTGCTGCTAGTTCTGGTGCTAGTGCTGCTGGTG
CTGTCGCTCCTGTCGCCAGCAACGTAGGAACGGCGGCGGTGGCGTCTCCC
CTAGGCCGGGTGTCCAAGCGCGTCGCCGCCCGGGCGGCAGAGCAGGCCAA
GGCGGCGGCGGCGGCGGCGGAGGCGGAGGCGGAGCTGGCGGCGGCGGCCC
TGGAAGCGAAGGCGCGCCGCAAAGAGGCCGCCGCCGCGGCTAAGAAGAGC
AAGAAGAAGGCTTCCAAGGCGGCGGCGGCGGCGACGACGACGATCATCTC
CGGAGATTTCCCACCGGTGGTCTCCGGAGATTCCCTCCCAGCGTCACACA
AGAAAGGGGTCACCATCGGTATGACTACTCCCACGGAAGGCGTTCCGCAT
GGCCCCAAGACGCGCGTCATATTGGGGAACATACCGCCGGCTGCTGGAAC
GGCGGGGAAGCCCGCCAAGCTAGCCTCGATCTTCCTTCCCAGGGCGGGCG
GCTTGGGGAGCGCGGCGCCTGCGGCGGGTCCGAAGCTGGCGCCGCTCTTC
ATGCCCAAGAGGAAGGCTAGCCCTGAGACCATTGGCGGCGGCAGCGGCGG
CGGCGGCGGCGGCGGCGGCGGCGGCGGTGGTCGTCGTAGTGGCGGTGGTG
GGGAGAAAGAGAAAGAGAAAGAAAAGAGCGGACGGAAAGAGGCTTCTCTT
CCTTCTTCGGCGGCTCCTCCGAGCATCGAGGTGCGCTAA