mRNA_Ecto-sp13_S_contig15723.4223.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig15723.4223.1
Unique NamemRNA_Ecto-sp13_S_contig15723.4223.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: D7FXW9_ECTSI (Intraflagellar transport protein 122 homolog n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXW9_ECTSI)

HSP 1 Score: 209 bits (531), Expect = 2.080e-60
Identity = 106/110 (96.36%), Postives = 107/110 (97.27%), Query Frame = 1 
Query:    1 LNSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            LNSVAKARFDDASYYYWRSSTEAFAAAEK+KTK SEKD RK QRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ
Sbjct:  932 LNSVAKARFDDASYYYWRSSTEAFAAAEKDKTKTSEKDIRKAQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 1041
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A7S4D6V4_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4D6V4_HETAK)

HSP 1 Score: 102 bits (253), Expect = 9.260e-24
Identity = 54/113 (47.79%), Postives = 78/113 (69.03%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAE----KEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V + RF DA+YYY+    E   AAE     +  ++SE   +K     + +++LA+LY A+ H++SFTTDPFT+LQPE+L QVSR+++N +G  + P GISR +TLYTLAKQ
Sbjct:   91 NAVMEGRFKDAAYYYYLLGAECLRAAEVLGEAKGGELSEAARKKALAEYDNYNKLANLYFAYQHIYSFTTDPFTNLQPEMLFQVSRYVLNLMGAEDAPYGISRVNTLYTLAKQ 203
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A7S3XTQ6_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XTQ6_HETAK)

HSP 1 Score: 102 bits (253), Expect = 2.020e-23
Identity = 54/113 (47.79%), Postives = 78/113 (69.03%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAE----KEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V + RF DA+YYY+    E   AAE     +  ++SE   +K     + +++LA+LY A+ H++SFTTDPFT+LQPE+L QVSR+++N +G  + P GISR +TLYTLAKQ
Sbjct:  153 NAVMEGRFKDAAYYYYLLGAECLRAAEVLGEAKGGELSEAARKKALAEYDNYNKLANLYFAYQHIYSFTTDPFTNLQPEMLFQVSRYVLNLMGAEDAPYGISRVNTLYTLAKQ 265
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A7R9U7I4_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U7I4_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 3.130e-17
Identity = 39/60 (65.00%), Postives = 47/60 (78.33%), Query Frame = 1 
Query:  151 RLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            R ADLY+AF  + SFT+DPFT L PEVL QV+R+++N LG +  P GISR HTLYTLAKQ
Sbjct:    1 RHADLYYAFQRIRSFTSDPFTSLLPEVLFQVARYIINCLGSSPAPYGISRVHTLYTLAKQ 60
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A812QAW2_SYMMI (Ift122 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812QAW2_SYMMI)

HSP 1 Score: 85.5 bits (210), Expect = 3.550e-17
Identity = 50/111 (45.05%), Postives = 68/111 (61.26%), Query Frame = 1 
Query:    1 LNSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEP-PRGISRAHTLYTLAKQ 330
            LN+V + RF DASYYYW  + +              + T    ++  EH+  ADLY+A+  + S+ TDPFT   PE+L QVSRF++NSLG +   P GIS+A TLYTLA+Q
Sbjct:  135 LNAVVETRFKDASYYYWLLAKDI------------REGTDSNYQLECEHN--ADLYYAYSSIQSYVTDPFTSQSPELLFQVSRFIINSLGSSGSIPYGISKAATLYTLARQ 231
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A8C2J6U0_CYPCA (Intraflagellar transport protein 122 homolog n=1 Tax=Cyprinus carpio TaxID=7962 RepID=A0A8C2J6U0_CYPCA)

HSP 1 Score: 84.3 bits (207), Expect = 1.010e-16
Identity = 45/109 (41.28%), Postives = 66/109 (60.55%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V ++RF+DASYYYW  S +    A ++K +M +K              LA+LYH +H VH + ++PF+   PE+L  +SRFL ++L   + P GIS+  TLY LAKQ
Sbjct:  867 NAVVESRFNDASYYYWMLSMQCLDIAREKKEEMLKK--------FHSFQHLAELYHVYHSVHRYMSEPFSSNMPEILFNISRFLFHNLTR-DAPMGISKVDTLYALAKQ 966
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A8C2J8G4_CYPCA (Intraflagellar transport protein 122 homolog n=3 Tax=Cyprinus carpio TaxID=7962 RepID=A0A8C2J8G4_CYPCA)

HSP 1 Score: 84.3 bits (207), Expect = 1.010e-16
Identity = 45/109 (41.28%), Postives = 66/109 (60.55%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V ++RF+DASYYYW  S +    A ++K +M +K              LA+LYH +H VH + ++PF+   PE+L  +SRFL ++L   + P GIS+  TLY LAKQ
Sbjct:  874 NAVVESRFNDASYYYWMLSMQCLDIAREKKEEMLKK--------FHSFQHLAELYHVYHSVHRYMSEPFSSNMPEILFNISRFLFHNLTR-DAPMGISKVDTLYALAKQ 973
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A8C1K7E3_CYPCA (Intraflagellar transport protein 122 homolog n=1 Tax=Cyprinus carpio TaxID=7962 RepID=A0A8C1K7E3_CYPCA)

HSP 1 Score: 84.3 bits (207), Expect = 1.010e-16
Identity = 45/109 (41.28%), Postives = 66/109 (60.55%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V ++RF+DASYYYW  S +    A ++K +M +K              LA+LYH +H VH + ++PF+   PE+L  +SRFL ++L   + P GIS+  TLY LAKQ
Sbjct:  884 NAVVESRFNDASYYYWMLSMQCLDIAREKKEEMLKK--------FHSFQHLAELYHVYHSVHRYMSEPFSSNMPEILFNISRFLFHNLTR-DAPMGISKVDTLYALAKQ 983
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: UPI0019555BAA (intraflagellar transport protein 122 homolog isoform X1 n=2 Tax=Pimephales promelas TaxID=90988 RepID=UPI0019555BAA)

HSP 1 Score: 82.8 bits (203), Expect = 3.510e-16
Identity = 46/111 (41.44%), Postives = 67/111 (60.36%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHS--RLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V ++RF DASYYYW  S +    A ++  K       K + + + HS   LA+LYH +H VH + ++PF+   PE+L  +SRFL ++L   + P GIS+  TLY LAKQ
Sbjct:  851 NAVVESRFSDASYYYWMLSMQCLDIAREDSEK-------KEEMLKKFHSFQHLAELYHVYHSVHRYMSEPFSSNMPEILFNISRFLFHNLTR-DVPMGISKVDTLYALAKQ 953
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BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Match: A0A8C2J8X8_CYPCA (Intraflagellar transport protein 122 homolog n=1 Tax=Cyprinus carpio TaxID=7962 RepID=A0A8C2J8X8_CYPCA)

HSP 1 Score: 82.4 bits (202), Expect = 4.750e-16
Identity = 45/109 (41.28%), Postives = 67/109 (61.47%), Query Frame = 1 
Query:    4 NSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHSRLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISRAHTLYTLAKQ 330
            N+V ++RF+DASYYYW  S +    A ++ T+  E+  +K          LA+LYH +H VH + ++PF+   PE+L  +SRFL ++L   + P GIS+  TLY LAKQ
Sbjct:  795 NAVVESRFNDASYYYWMLSMQCLDIA-RDNTEKKEEMLKKFHSF----QHLAELYHVYHSVHRYMSEPFSSNMPEILFNISRFLFHNLTR-DAPMGISKVDTLYALAKQ 897
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig15723.4223.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FXW9_ECTSI2.080e-6096.36Intraflagellar transport protein 122 homolog n=1 T... [more]
A0A7S4D6V4_HETAK9.260e-2447.79Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S3XTQ6_HETAK2.020e-2347.79Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7R9U7I4_9STRA3.130e-1765.00Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A812QAW2_SYMMI3.550e-1745.05Ift122 protein n=1 Tax=Symbiodinium microadriaticu... [more]
A0A8C2J6U0_CYPCA1.010e-1641.28Intraflagellar transport protein 122 homolog n=1 T... [more]
A0A8C2J8G4_CYPCA1.010e-1641.28Intraflagellar transport protein 122 homolog n=3 T... [more]
A0A8C1K7E3_CYPCA1.010e-1641.28Intraflagellar transport protein 122 homolog n=1 T... [more]
UPI0019555BAA3.510e-1641.44intraflagellar transport protein 122 homolog isofo... [more]
A0A8C2J8X8_CYPCA4.750e-1641.28Intraflagellar transport protein 122 homolog n=1 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig15723contigEcto-sp13_S_contig15723:2669..4088 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score210.7
Seed ortholog evalue3.5e-52
Seed eggNOG ortholog2880.D7FXW9
Preferred nameIFT122
Model size332
KEGG koko:K16547,ko:K19656
Hectar predicted targeting categoryother localisation
GOsGO:0001654,GO:0001838,GO:0001841,GO:0001843,GO:0002009,GO:0003002,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005815,GO:0005829,GO:0005856,GO:0005929,GO:0006810,GO:0006928,GO:0006996,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007166,GO:0007224,GO:0007227,GO:0007275,GO:0007389,GO:0007399,GO:0007417,GO:0007423,GO:0007507,GO:0008104,GO:0008150,GO:0008285,GO:0008589,GO:0009653,GO:0009790,GO:0009792,GO:0009887,GO:0009888,GO:0009953,GO:0009966,GO:0009968,GO:0009987,GO:0010171,GO:0010172,GO:0010646,GO:0010648,GO:0010970,GO:0014020,GO:0015630,GO:0016043,GO:0016331,GO:0021510,GO:0021511,GO:0021513,GO:0021532,GO:0021904,GO:0021914,GO:0021915,GO:0022607,GO:0023051,GO:0023052,GO:0023057,GO:0030030,GO:0030031,GO:0030326,GO:0030705,GO:0030990,GO:0030991,GO:0031503,GO:0031974,GO:0031981,GO:0032391,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0035050,GO:0035107,GO:0035108,GO:0035113,GO:0035115,GO:0035136,GO:0035148,GO:0035239,GO:0035295,GO:0035556,GO:0035720,GO:0035721,GO:0035735,GO:0035869,GO:0036064,GO:0042073,GO:0042127,GO:0042733,GO:0042995,GO:0043005,GO:0043009,GO:0043010,GO:0043113,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044422,GO:0044424,GO:0044428,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044463,GO:0044464,GO:0044782,GO:0045184,GO:0045879,GO:0046626,GO:0046907,GO:0048513,GO:0048518,GO:0048519,GO:0048523,GO:0048568,GO:0048580,GO:0048582,GO:0048583,GO:0048585,GO:0048592,GO:0048593,GO:0048598,GO:0048646,GO:0048729,GO:0048731,GO:0048736,GO:0048856,GO:0050678,GO:0050680,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051093,GO:0051094,GO:0051179,GO:0051234,GO:0051239,GO:0051240,GO:0051241,GO:0051641,GO:0051649,GO:0051668,GO:0051716,GO:0051960,GO:0051961,GO:0060173,GO:0060271,GO:0060429,GO:0060562,GO:0060606,GO:0060830,GO:0060831,GO:0060971,GO:0060972,GO:0061062,GO:0061063,GO:0061065,GO:0061066,GO:0061512,GO:0065007,GO:0070013,GO:0070727,GO:0070925,GO:0071840,GO:0072175,GO:0072359,GO:0072594,GO:0072657,GO:0090596,GO:0097458,GO:0097499,GO:0097500,GO:0097542,GO:0097546,GO:0097730,GO:0097731,GO:0097733,GO:0099111,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1900076,GO:1903441,GO:1905515,GO:1990778,GO:2000026
Exons3
EggNOG free text desc.ciliary receptor clustering involved in smoothened signaling pathway
EggNOG OGsCOG2319@1,KOG1538@2759
Cds size330
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko04812
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462831.634227-CDS-Ecto-sp13_S_contig15723:2668..27721681462831.634227-CDS-Ecto-sp13_S_contig15723:2668..2772Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 2669..2772 +
1681462831.6486096-CDS-Ecto-sp13_S_contig15723:3040..31761681462831.6486096-CDS-Ecto-sp13_S_contig15723:3040..3176Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 3041..3176 +
1681462831.663956-CDS-Ecto-sp13_S_contig15723:3997..40871681462831.663956-CDS-Ecto-sp13_S_contig15723:3997..4087Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15723 3998..4087 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig15723.4223.1prot_Ecto-sp13_S_contig15723.4223.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig15723 2669..4087 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig15723.4223.1

>prot_Ecto-sp13_S_contig15723.4223.1 ID=prot_Ecto-sp13_S_contig15723.4223.1|Name=mRNA_Ecto-sp13_S_contig15723.4223.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=110bp
LNSVAKARFDDASYYYWRSSTEAFAAAEKEKTKMSEKDTRKTQRIAEEHS
RLADLYHAFHHVHSFTTDPFTDLQPEVLLQVSRFLVNSLGEAEPPRGISR
AHTLYTLAKQ
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mRNA from alignment at Ecto-sp13_S_contig15723:2669..4088+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig15723.4223.1 ID=mRNA_Ecto-sp13_S_contig15723.4223.1|Name=mRNA_Ecto-sp13_S_contig15723.4223.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1420bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15723:2669..4088+ (Ectocarpus species13 EcNAP12_S_4_19m)
CTCAACTCCGTGGCGAAAGCCCGGTTCGACGACGCTTCTTACTACTATTG
GAGGTCTTCGACTGAGGCTTTCGCAGCAGCAGAGAAGGAAAAAACGAAAA
TGAGGTATGTGTATATGTGTGTGTGGTGGATGGACCCAACCAAGGAGCAG
CGTGCCGACTCTATCAAACACCAAACCCTGCAGTTCGCGCGTTGCGTTAG
CCCTGGTTTGCCTTGATGCCCTATGTTCTTGTGGCTTTGTTACCCTCCTA
CGTAGATCGTATGTTCGGAGAAATGATGCTTTGATTTTCCCGTTGTCGAT
GCTCTTTTCGCTCTTTTCGGATTGTCATGTTCTTGTGAACCGCATTATTC
ACGATACTGTCGACATCTGCAGCGAGAAGGACACCAGAAAGACTCAGAGG
ATTGCGGAGGAGCACTCTCGTTTGGCGGACTTGTATCACGCGTTTCACCA
CGTGCACTCTTTCACCACCGATCCCTTCACGGACCTACAGCCGGAGGTCT
TGCTGCAGGTGATGAGAGACGACGGCGTGACTTAGCACTTGGATGATCCT
GGCTTGAAGACAACGTTGAAATGGCGTTTGGGAATTCGCTGTTTCGGTTA
ACGTTCCTGTTTCGAGGCAGTGTTCACCGATCCTGTTTTTCCAACCTTAC
TGTCATCATGTGAACGAAACAACCTCGCCTTTGAAAGCTTCACCGAGCGA
TAACCAAACCTTTTGCTCGATCTAGTGTGAGCTAGCTCACCTGCTGTTCC
TTTATGCTAAATGACTTCCGCTCCTATCCAGGGCTAGCTGTCGTTACTAT
GGGCTGCCAGGCAAACCTACAGACAGGCGCTTAGGCAGGATGTCGCATCA
ACAAAAGGACAGCTGACTGTAGACAGAGTGTCTCTCCTTGACTTTCAGAA
ACGGGACCATGCTATGAAAAGTAGACCGTGCCACTTCAGTCACGAAATTG
CCTTAATACCAAACCACGGCCCGGAAATTCCCTCATAGGAGTAGTCATGG
TTCATGCGGGAAAGTTTTCCAGAAATGCCAAGGGGGCGTAGGCCGTGGGG
CAGAGTTGCAAGTTTGCGGTTCAGGACTTCGTAGGTATCCTTGCCAACTT
AAGGTTGCCGAACAGGTGTTACGTCGATACAATCTGCCTAGCTTTGCTTT
TCTCTTCGGTGATCGTGTTCGTCATCGATCACAGCACCATTAGTTGCCGA
ACGACAAGAATGGCAACAGCCAACACCACGCAGTATGACATGGTACGTAG
CGTCCAGGTACACCAGGTACTCACTCTGATCACTTCATTCTCCGCCCTTC
CTCTGCTTTCACCCGGCTGCTTGGCGTAGGTGTCGAGGTTCCTCGTTAAT
TCCTTGGGCGAAGCGGAGCCGCCTCGCGGGATATCAAGGGCACACACTCT
CTACACCTTGGCCAAGCAGG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig15723:2669..4088+

>mRNA_Ecto-sp13_S_contig15723.4223.1 ID=mRNA_Ecto-sp13_S_contig15723.4223.1|Name=mRNA_Ecto-sp13_S_contig15723.4223.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=330bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15723:2669..4088+ (Ectocarpus species13 EcNAP12_S_4_19m)
CTCAACTCCGTGGCGAAAGCCCGGTTCGACGACGCTTCTTACTACTATTG
GAGGTCTTCGACTGAGGCTTTCGCAGCAGCAGAGAAGGAAAAAACGAAAA
TGAGCGAGAAGGACACCAGAAAGACTCAGAGGATTGCGGAGGAGCACTCT
CGTTTGGCGGACTTGTATCACGCGTTTCACCACGTGCACTCTTTCACCAC
CGATCCCTTCACGGACCTACAGCCGGAGGTCTTGCTGCAGGTGTCGAGGT
TCCTCGTTAATTCCTTGGGCGAAGCGGAGCCGCCTCGCGGGATATCAAGG
GCACACACTCTCTACACCTTGGCCAAGCAG
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