mRNA_Ecto-sp13_S_contig15381.4036.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig15381.4036.1
Unique NamemRNA_Ecto-sp13_S_contig15381.4036.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Match: D8LRK2_ECTSI (WSC domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LRK2_ECTSI)

HSP 1 Score: 587 bits (1514), Expect = 2.800e-205
Identity = 286/312 (91.67%), Postives = 292/312 (93.59%), Query Frame = 1 
Query:   40 MLKKCCFALVALLPTTAVSASQLTTTDACNFTFSDGELPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGERVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWAAVDSRYGSTEESDDPCTDYDGILDADGVTCCSDEDTTCDSGDVDSDDRFCGDDNPPPCEL 975
            MLKKCCFALVALL TTAVSASQ TTTDACNFTFSDG+LPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAY VGDDTWNTTY+GNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGE+VQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWS+WWA VDSRYGSTEESDDPCT+YDGI+DADGV CCSDED TCDSG             P PCEL
Sbjct:    1 MLKKCCFALVALLSTTAVSASQFTTTDACNFTFSDGQLPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYQVGDDTWNTTYVGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGEKVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSNWWADVDSRYGSTEESDDPCTEYDGIVDADGVYCCSDEDNTCDSGXXXXXXXXXXXXXPAPCEL 312
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BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Match: D7G672_ECTSI (WSC domain-containing protein n=3 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G672_ECTSI)

HSP 1 Score: 580 bits (1495), Expect = 5.440e-202
Identity = 278/289 (96.19%), Postives = 286/289 (98.96%), Query Frame = 1 
Query:   40 MLKKCCFALVALLPTTAVSASQLTTTDACNFTFSDGELPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGERVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWAAVDSRYGSTEESDDPCTDYDGILDADGVTCCSDEDTT 906
            MLKKCCFALVALLPTTAVSASQ TTTDACNFTFSDG+LPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGE+VQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWS+WWA VDSRYGSTEESDDPCT++DGI+DADGV CCSDE++T
Sbjct:    1 MLKKCCFALVALLPTTAVSASQFTTTDACNFTFSDGQLPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGEKVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSNWWAVVDSRYGSTEESDDPCTEHDGIVDADGVYCCSDEEST 289
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BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Match: D7FS82_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS82_ECTSI)

HSP 1 Score: 553 bits (1425), Expect = 3.950e-198
Identity = 268/277 (96.75%), Postives = 270/277 (97.47%), Query Frame = 1 
Query:    1 RHEATTSLYNSTAMLKKCCFALVALLPTTAVSASQLTTTDACNFTFSDGELPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGERVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWAAVDSRYGSTEES 831
            RHEATTSLY  TAMLK CCFALVALLPTTAVSASQ TTTD CNFTFSDG+LPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGE+VQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWA VDSRY STEES
Sbjct:    2 RHEATTSLYKGTAMLKICCFALVALLPTTAVSASQFTTTDGCNFTFSDGQLPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGEQVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWATVDSRYVSTEES 278
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BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Match: D7G7E4_ECTSI (WSC domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7E4_ECTSI)

HSP 1 Score: 446 bits (1147), Expect = 1.390e-150
Identity = 216/240 (90.00%), Postives = 221/240 (92.08%), Query Frame = 1 
Query:  256 NGTLHSCNGESSAYHVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGERVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNEDGSRKDDEFWSDWWAAVDSRYGSTEESDDPCTDYDGILDADGVTCCSDEDTTCDSGDVDSDDRFCGDDNPPPCEL 975
            NGTLHSCNGESSAYHVGDDTWNTTYIGNDGD+L VDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGE+VQSVVVHAASPIGPTSSASLGVGNALILAGKADQG PWSEGFNEDGSRKDDEFWSDWWA VDSRYGSTEESDDPCT+YDGI+DADGV CC DED+TCDSGD              PCEL
Sbjct:   13 NGTLHSCNGESSAYHVGDDTWNTTYIGNDGDKLGVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHGFSSGEQVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGPPWSEGFNEDGSRKDDEFWSDWWAVVDSRYGSTEESDDPCTEYDGIVDADGVYCCFDEDSTCDSGDXXXXXXXXXXXXXAPCEL 252
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BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Match: A0A814YKZ3_9BILA (Hypothetical protein n=1 Tax=Didymodactylos carnosus TaxID=1234261 RepID=A0A814YKZ3_9BILA)

HSP 1 Score: 84.0 bits (206), Expect = 3.980e-15
Identity = 63/157 (40.13%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:   88 AVSASQLTTTDACNFTFSDGELPNFRLPEAGPNSPGDVQLNLGNNI-ITRGWVEEDENGTLHSCNGESS----AY-HVGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALNDTTIRLNITCAKMDHSSSLQNGCLEFTVNFT 540
            A++ S   T   C ++F+  + P+F LP A PNSPGD QLNL NNI ++ G       G    CNG  S    AY +  +  W T+Y   +G   +++VFYAT Q+ L V  LQ +   +L   L   TIRLNITC      ++ Q GC+ FTVN T
Sbjct:   23 AITVSLANTCANCLYSFTAPQFPSFTLPAAQPNSPGDQQLNLNNNIRLSNGSALNAGQG----CNGGRSSGEYAYLNESNGQWMTSYPDVNGRPADLNVFYATAQSALNVATLQTNY-ANLALFLTCKTIRLNITC------NNGQKGCIYFTVNCT 168
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig15381.4036.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D8LRK2_ECTSI2.800e-20591.67WSC domain-containing protein n=2 Tax=Ectocarpus T... [more]
D7G672_ECTSI5.440e-20296.19WSC domain-containing protein n=3 Tax=Ectocarpus s... [more]
D7FS82_ECTSI3.950e-19896.75Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G7E4_ECTSI1.390e-15090.00WSC domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A814YKZ3_9BILA3.980e-1540.13Hypothetical protein n=1 Tax=Didymodactylos carnos... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig15381contigEcto-sp13_S_contig15381:75..2846 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Model size975
Hectar predicted targeting categorysignal peptide
Exons4
Cds size975
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462818.139185-CDS-Ecto-sp13_S_contig15381:74..2171681462818.139185-CDS-Ecto-sp13_S_contig15381:74..217Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15381 75..217 -
1681462818.1490188-CDS-Ecto-sp13_S_contig15381:862..12831681462818.1490188-CDS-Ecto-sp13_S_contig15381:862..1283Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15381 863..1283 -
1681462818.159278-CDS-Ecto-sp13_S_contig15381:2033..21881681462818.159278-CDS-Ecto-sp13_S_contig15381:2033..2188Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15381 2034..2188 -
1681462818.170936-CDS-Ecto-sp13_S_contig15381:2590..28461681462818.170936-CDS-Ecto-sp13_S_contig15381:2590..2846Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig15381 2591..2846 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig15381.4036.1prot_Ecto-sp13_S_contig15381.4036.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig15381 75..2846 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig15381.4036.1

>prot_Ecto-sp13_S_contig15381.4036.1 ID=prot_Ecto-sp13_S_contig15381.4036.1|Name=mRNA_Ecto-sp13_S_contig15381.4036.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=325bp
RHEATTSLYNSTAMLKKCCFALVALLPTTAVSASQLTTTDACNFTFSDGE
LPNFRLPEAGPNSPGDVQLNLGNNIITRGWVEEDENGTLHSCNGESSAYH
VGDDTWNTTYIGNDGDELEVDVFYATYQNQLWVRHLQPHMDPSLYEALND
TTIRLNITCAKMDHSSSLQNGCLEFTVNFTEATPYNYTEPYEFVDDCSHG
FSSGERVQSVVVHAASPIGPTSSASLGVGNALILAGKADQGLPWSEGFNE
DGSRKDDEFWSDWWAAVDSRYGSTEESDDPCTDYDGILDADGVTCCSDED
TTCDSGDVDSDDRFCGDDNPPPCEL
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mRNA from alignment at Ecto-sp13_S_contig15381:75..2846-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig15381.4036.1 ID=mRNA_Ecto-sp13_S_contig15381.4036.1|Name=mRNA_Ecto-sp13_S_contig15381.4036.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2772bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15381:75..2846- (Ectocarpus species13 EcNAP12_S_4_19m)
CGCCACGAAGCGACGACTAGTCTATACAACAGCACAGCCATGCTAAAAAA
ATGCTGCTTCGCGTTGGTAGCGTTGCTACCCACGACGGCGGTGTCCGCCT
CCCAGCTTACGACGACGGACGCCTGCAACTTCACTTTCAGCGATGGAGAG
TTGCCGAACTTCCGCTTGCCGGAGGCAGGGCCAAACAGCCCTGGTGACGT
CCAGCTCAACCTCGGGAACAACATCATCACACGAGGATGGGTGGAGGAAG
ACGAAAGTGAGTCAATTTCGACGTTATTACAGAGTGCGTAAACGTATCAA
GCGAGAACGCGGAGGTGACGGAAAGAAAATGCCACGTCGTTGGCATGAAA
TTGTCATGTATATACTAATTGTAGTCAACACTGGTGCGCGTGCCTCACCG
TGCACTTGTGTACAGCACTCGAGCAGCGGTAGTCGATGCAGGTGCGCACT
GACTTTGGCTGGCGCACGCATTGGAATGTCTGTGACGTGCGTGCGGTACG
TCAACGGACGGTGCCAGCCGGCGGTTTATTGGAGGGCAAGGGTGCCAGTC
TCGTACGAATAGCACCCATATACTAAAGCGAATGCTATTGCTGCATAATG
ATAAATATGTTCACGTTCAATACCTGTGTTGTTCTCTGCTCATCACGTAC
AACAACAGACGGAACCTTGCACAGCTGCAACGGTGAATCGAGTGCTTACC
ATGTAGGGGACGACACTTGGAACACCACATACATTGGGAACGACGGCGAT
GAGCTCGAAGTTGACGTGTTCTACGCAACCTACCAAAATCAGCTCTGGGT
CCGCCACCTCCAGGTATGTACCCATTTTGAGAATTATGAATTTCATCGAT
GCCAAACATTTGGCGTGGCTATATCACTTTTTTGTGACCACGGACCGAGT
CTTTGCTAGATTTTGGTATAAGCAACCGACGCGAGTTTATGTGAAGAAAA
CAACAACAACAACATGGCAAACAAGAAAGATGTTCTTCCATCGACAGCAA
TGGATGACCCCCGAAACAAAATACCTATACTTCCCCCAAACACGAGGCTA
GTGGCCTCGTGTATGCCACTCCTGAACTCTAGCAGCGCCCGTGTATCTGC
CTCACAAGCATACCTAGCCGCAACACGTTGTTTTGCTCCCGGTGCAAATT
CTACTTTTTTATGCCCTCCATCCTGTTTCCAGCGCGACCCAGCTCCGTAA
TATCGCTTCACGCTGAACAGTTGCGAATGGAAAGACATGCACGTTCGCCT
TGTGCTCCACCTTATTACCGTAGTAGGTGGCCTAAGATGTCACACCACCT
GAACGGGATTTCTAAGCTCGAATGCTCATGAGGCAAGAACGGCGAGGGTG
CCGAAAATGAAAGGGTTGAGAGCTTTCCGCGAACGATCCGTGTGGTTTTG
GCAATCTCTTCGTTGTGGAGTAATCGAGATTTTAATTTCGGCCCAGGGAG
TCTGGTATCTTATGCGACCTACGGTATCCCAATACTGGGGGCACCACTCT
TCTTCTGTGTCTTGGAGACAATCACCCCCTCCCTCTCCCGTTGATCGACA
ACTATTCCGTCAGCCCCACATGGACCCAAGTCTGTACGAGGCCCTCAACG
ACACCACGATCCGGCTGAACATCACATGCGCGAAGATGGACCATTCCAGC
TCTCTGCAGAACGGCTGCCTTGAGTTCACGGTCAACTTCACGGAGGCCAC
ACCTTACAACTACACCGAACCATACGAGTTCGTCGACGACTGCTCTCACG
GATTTTCCAGCGGTGAACGGGTGCAGTCCGTTGTAGTGCACGCGGCGTCA
CCCATAGGGCCCACGAGCTCGGCTTCCCTGGGCGTCGGAAACGCGCTCAT
TTTGGCCGGAAAGGCAGACCAGGGTCTCCCCTGGTCGGAAGGGTTTAATG
AGGACGGCAGCCGGAAAGATGACGAGTTTTGGAGCGACTGGTGGGCCGCC
GTCGATAGTCGGTACGGTAGTACCGAAGAGAGTGGTGAGTGCCAGGCGTG
TACGACAGATTCTATTCAAATGGTGGGCACGTTGTTTCTGCTGTCTATTG
ACTTCAACGAACTTGGCACTTGCAGCAGCGAGCCAAAAGCGCGGTTCGCT
GATGTGCCTCATGATGTATATTCGTACAAAATTATGGTCGTTTCTGTTGA
TACTGGTCACCGGTCGAAAGTTGGTGACGGCATGCTGAGCAGAAATCAAT
GCAACAAGCGGTTCGCACCACCAACACGCCGATTTCATATCCTTAGCCTT
AATTGCTAAGTCTTGGCGAAACTCAGTATTCAAACAACACAGCGTAGGGG
CCGCAAAACATGCCTTCATTTTAGAAGATGAGAAAGAGAACTGTTCTTGT
GTTCGATTTTGCGGAGACAATGTCCGCACCAATAACGTATGGAAAAACGC
ACGCCGCGCGCCTGAAACACGATTTCTTGCTTCGTTTGGGGTCATGAGAT
CTGATTGTGCGGGTACTTCGTGTGCCTATTTCATAAGTAGCATCGATTGC
CCCGCGTTTGCACGTACAGTACCACCCCTCGACGACTGGATTTGTCGTGT
TTACAGAGTCGAACTTTCCCAACACAACGGCCCGTCTAACTTGCGCTGCC
CCCTAACTGACCCGCTCTGCCACGAACAGATGATCCGTGCACCGACTATG
ACGGAATCCTCGATGCTGATGGTGTCACTTGCTGCTCTGACGAGGACACC
ACCTGCGACTCGGGAGATGTTGACAGTGACGATAGGTTCTGCGGTGATGA
TAACCCACCGCCCTGCGAACTA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig15381:75..2846-

>mRNA_Ecto-sp13_S_contig15381.4036.1 ID=mRNA_Ecto-sp13_S_contig15381.4036.1|Name=mRNA_Ecto-sp13_S_contig15381.4036.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=975bp|location=Sequence derived from alignment at Ecto-sp13_S_contig15381:75..2846- (Ectocarpus species13 EcNAP12_S_4_19m)
CGCCACGAAGCGACGACTAGTCTATACAACAGCACAGCCATGCTAAAAAA
ATGCTGCTTCGCGTTGGTAGCGTTGCTACCCACGACGGCGGTGTCCGCCT
CCCAGCTTACGACGACGGACGCCTGCAACTTCACTTTCAGCGATGGAGAG
TTGCCGAACTTCCGCTTGCCGGAGGCAGGGCCAAACAGCCCTGGTGACGT
CCAGCTCAACCTCGGGAACAACATCATCACACGAGGATGGGTGGAGGAAG
ACGAAAACGGAACCTTGCACAGCTGCAACGGTGAATCGAGTGCTTACCAT
GTAGGGGACGACACTTGGAACACCACATACATTGGGAACGACGGCGATGA
GCTCGAAGTTGACGTGTTCTACGCAACCTACCAAAATCAGCTCTGGGTCC
GCCACCTCCAGCCCCACATGGACCCAAGTCTGTACGAGGCCCTCAACGAC
ACCACGATCCGGCTGAACATCACATGCGCGAAGATGGACCATTCCAGCTC
TCTGCAGAACGGCTGCCTTGAGTTCACGGTCAACTTCACGGAGGCCACAC
CTTACAACTACACCGAACCATACGAGTTCGTCGACGACTGCTCTCACGGA
TTTTCCAGCGGTGAACGGGTGCAGTCCGTTGTAGTGCACGCGGCGTCACC
CATAGGGCCCACGAGCTCGGCTTCCCTGGGCGTCGGAAACGCGCTCATTT
TGGCCGGAAAGGCAGACCAGGGTCTCCCCTGGTCGGAAGGGTTTAATGAG
GACGGCAGCCGGAAAGATGACGAGTTTTGGAGCGACTGGTGGGCCGCCGT
CGATAGTCGGTACGGTAGTACCGAAGAGAGTGATGATCCGTGCACCGACT
ATGACGGAATCCTCGATGCTGATGGTGTCACTTGCTGCTCTGACGAGGAC
ACCACCTGCGACTCGGGAGATGTTGACAGTGACGATAGGTTCTGCGGTGA
TGATAACCCACCGCCCTGCGAACTA
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