mRNA_Ecto-sp13_S_contig14587.3517.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig14587.3517.1
Unique NamemRNA_Ecto-sp13_S_contig14587.3517.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig14587.3517.1 vs. uniprot
Match: D7FKD6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FKD6_ECTSI)

HSP 1 Score: 686 bits (1770), Expect = 2.110e-243
Identity = 357/393 (90.84%), Postives = 363/393 (92.37%), Query Frame = 1 
Query:    1 GRFGKGPATRAGGLASTMPVQGTLGQRPKPFFATALMLSRSDDSPASEGGSPFEGWGAGKSRRGTGRTDSIFTQGPNDPGVEAVIQAKVTTLISRRGPELEALLLLRDPERSGNIPVKAFSECLRAWVSSDSERGGGGAADHTRAGKRDGPSQAAELTHADRKVLYRRWAVRGQVRYVDFLGASGYFNAPLPRYSEISTAVAAAPAASAAPVVPDKLARHSVIGKRASGRLPSGDTTGMNARRADAGEEETGLLARARVVLVHLSEVLRDEGEEVYFAAFEAHDYGNYRCLDEDGFVDAIGTLAPEVTAEQVHDVFLVRSRIEASDDKANYDELFKDLRKLAQIGGFLSGDAVELRVESRGGRVDWVPAQVVSRDGDGTYTVRFKSPTKVPGA 1179
            GRFGK P TRAGGLA TMPVQGTLGQRPKPFFATALMLSRSDD PASE GSPF+GWGAGKSRRGTGRTDSIFTQGPNDPGVEA IQ KV+TLISRRGPELEALLLLRDPERSGNIPVKAFSECLRAW SSDSERGGGGA   TRAGKR+GPSQAAELTHADRKVLYRRW VRGQVRYVDFLGASGYFNAPLPRYSEISTAVAAAPAA+AAPVV D  ARH V  KRASG L SGD  GM+AR ADAGEEETGLL R+RVVLVHLSEVLRDEGEE YFAAFEA DYGNYRCLDEDGFVDAI TLAPEVTAEQVHDVFLVRSRIE SDDKANYDELFKDLRKLAQIGGF SGDAVELRVESRG RVDWVPAQVVSRDGDGTYTVRFKSPTKVPGA
Sbjct:  241 GRFGKCPTTRAGGLACTMPVQGTLGQRPKPFFATALMLSRSDDPPASERGSPFDGWGAGKSRRGTGRTDSIFTQGPNDPGVEAPIQEKVSTLISRRGPELEALLLLRDPERSGNIPVKAFSECLRAWASSDSERGGGGAG--TRAGKREGPSQAAELTHADRKVLYRRWVVRGQVRYVDFLGASGYFNAPLPRYSEISTAVAAAPAAAAAPVVTDAPARHPVTRKRASGSLLSGDKAGMHARNADAGEEETGLLERSRVVLVHLSEVLRDEGEEAYFAAFEAQDYGNYRCLDEDGFVDAIETLAPEVTAEQVHDVFLVRSRIEGSDDKANYDELFKDLRKLAQIGGFQSGDAVELRVESRGSRVDWVPAQVVSRDGDGTYTVRFKSPTKVPGA 631
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BLAST of mRNA_Ecto-sp13_S_contig14587.3517.1 vs. uniprot
Match: A0A6H5JE59_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JE59_9PHAE)

HSP 1 Score: 594 bits (1532), Expect = 4.820e-211
Identity = 314/375 (83.73%), Postives = 324/375 (86.40%), Query Frame = 1 
Query:  109 MLSRSDDSPASEGGSPFEGWGAGKSRRGTGRTDSIFTQGPNDPGVEAVIQAKVTTLISRRGPELEALLLLRDPERSGNIPVKAFSECLRAWVSSDSERGGGGAADHTRAGKRDGPSQAAELTHADRKVLYRRWAVRGQVRYVDFLGASGYFNAPLPRYSEISTAVAAAPAASAAPVVPDKLARHSVIGKRASGRLPSGDTTGMNARRADAGEEETGLLARARVVLVHLSEVLRDEGEEVYFAAFEAHDYGNYRCLDEDGFVDAIGTLAPEVTAEQVHDVFLVRSRIEASDDKANYDELFK------------------DLRKLAQIGGFLSGDAVELRVESRGGRVDWVPAQVVSRDGDGTYTVRFKSPTKVPGA 1179
            MLSRSDDSPASEGGSPF+GWGAGK+RRGTGRTDSIFTQG NDPGVEA +Q K+ TLISRRGPELEALLLLRDPERSG+I VK FSECLRAWVSSDSE GGGG    TR GKR+GPSQAAELTHADRKVLYRRWAVRGQVRYVDFLGASG FNAPLPRYSEISTAVAAAP      VV DK ARH V GKRASGRLPSGD  GM+AR ADAGEEET L ARARVVLVHLSEVLRDEGEE YFAAFEA DYGNYRCLDEDGFV+AI TLAPEVTAEQVHDVFLVRSRIE SDDKANYDELFK                  DLRKLAQIGGF SGDAVELRVESRGGRVDWVPAQVVSRDGDGT+TVRF+SPTKVPGA
Sbjct:    1 MLSRSDDSPASEGGSPFDGWGAGKNRRGTGRTDSIFTQGRNDPGVEAAVQEKLATLISRRGPELEALLLLRDPERSGSISVKTFSECLRAWVSSDSEHGGGGTG--TRGGKREGPSQAAELTHADRKVLYRRWAVRGQVRYVDFLGASGCFNAPLPRYSEISTAVAAAPXXXXXXVVADKPARHPVTGKRASGRLPSGDKAGMHARNADAGEEETDLFARARVVLVHLSEVLRDEGEEAYFAAFEAQDYGNYRCLDEDGFVNAIETLAPEVTAEQVHDVFLVRSRIEGSDDKANYDELFKLGCAFPYPSPRPSITARKDLRKLAQIGGFQSGDAVELRVESRGGRVDWVPAQVVSRDGDGTFTVRFRSPTKVPGA 373
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig14587.3517.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7FKD6_ECTSI2.110e-24390.84Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JE59_9PHAE4.820e-21183.73Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig14587contigEcto-sp13_S_contig14587:15..4380 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Model size1179
Hectar predicted targeting categoryother localisation
Exons7
Cds size1179
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig14587.3517.1prot_Ecto-sp13_S_contig14587.3517.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig14587 15..4380 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462778.6143775-CDS-Ecto-sp13_S_contig14587:14..921681462778.6143775-CDS-Ecto-sp13_S_contig14587:14..92Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 15..92 +
1681462778.6253421-CDS-Ecto-sp13_S_contig14587:546..6911681462778.6253421-CDS-Ecto-sp13_S_contig14587:546..691Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 547..691 +
1681462778.6350572-CDS-Ecto-sp13_S_contig14587:1140..17151681462778.6350572-CDS-Ecto-sp13_S_contig14587:1140..1715Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 1141..1715 +
1681462778.6448402-CDS-Ecto-sp13_S_contig14587:2014..21321681462778.6448402-CDS-Ecto-sp13_S_contig14587:2014..2132Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 2015..2132 +
1681462778.6542408-CDS-Ecto-sp13_S_contig14587:2562..26541681462778.6542408-CDS-Ecto-sp13_S_contig14587:2562..2654Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 2563..2654 +
1681462778.6641572-CDS-Ecto-sp13_S_contig14587:3361..34631681462778.6641572-CDS-Ecto-sp13_S_contig14587:3361..3463Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 3362..3463 +
1681462778.672404-CDS-Ecto-sp13_S_contig14587:4311..43801681462778.672404-CDS-Ecto-sp13_S_contig14587:4311..4380Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14587 4312..4380 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig14587.3517.1

>prot_Ecto-sp13_S_contig14587.3517.1 ID=prot_Ecto-sp13_S_contig14587.3517.1|Name=mRNA_Ecto-sp13_S_contig14587.3517.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=393bp
GRFGKGPATRAGGLASTMPVQGTLGQRPKPFFATALMLSRSDDSPASEGG
SPFEGWGAGKSRRGTGRTDSIFTQGPNDPGVEAVIQAKVTTLISRRGPEL
EALLLLRDPERSGNIPVKAFSECLRAWVSSDSERGGGGAADHTRAGKRDG
PSQAAELTHADRKVLYRRWAVRGQVRYVDFLGASGYFNAPLPRYSEISTA
VAAAPAASAAPVVPDKLARHSVIGKRASGRLPSGDTTGMNARRADAGEEE
TGLLARARVVLVHLSEVLRDEGEEVYFAAFEAHDYGNYRCLDEDGFVDAI
GTLAPEVTAEQVHDVFLVRSRIEASDDKANYDELFKDLRKLAQIGGFLSG
DAVELRVESRGGRVDWVPAQVVSRDGDGTYTVRFKSPTKVPGA
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mRNA from alignment at Ecto-sp13_S_contig14587:15..4380+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig14587.3517.1 ID=mRNA_Ecto-sp13_S_contig14587.3517.1|Name=mRNA_Ecto-sp13_S_contig14587.3517.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4366bp|location=Sequence derived from alignment at Ecto-sp13_S_contig14587:15..4380+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGGAGATTCGGCAAAGGTCCCGCCACGAGAGCGGGTGGGCTAGCATCCAC
GATGCCTGTGCAGGGTACATTGGGACAGGTGTGTGGCAATAAAAAGCAGT
GCCGCCTTCGCTCTGACTCGCGGCTGAATCTCATGGTGCCGCAACTGTAC
ACCAGTCCCGGTGTCGAAATGGGGTGGCCGCACTCGCCTCGTTCTCATCC
CTTGGGGAACAACGGTGTGCTTCTGTTTTTTGGTGAGATGCGGTAGCTGC
GGACAATACACGACAAAGCTTTCTGATAGGCGGGCGTCGACGACGTGGGA
AATTATCGTCGAAGATCCATCGCCACATACAGCTGGTGCTGCAGACGGTT
TATCATGTGCTCGACAAATTCCGCAGCCATCCATGTTTCGGACTGACCGG
TTTGTTCGTTTCTTCGCCGCCCTTGTTCTTCACCCCCCCCGCGTGCCCGA
ACGCTGATCCCCCGCACGAACCAACGGACAACTTACCGAACACAACCAAC
ACCAACATCGACCACACGGGGCGTCTTCCAAGCGCCCAAAACCGTTCTTC
GCGACGGCTCTAATGCTGTCCCGATCGGACGACTCGCCTGCGTCGGAGGG
AGGCTCGCCTTTCGAAGGGTGGGGGGCGGGCAAAAGCCGTCGGGGCACAG
GCAGAACAGACTCCATCTTTACTCAAGGTACCCAAATCTCGACTGCTGAG
GCCCTCGCTCAACGAGAACGGAGTTATTTGTCTTGCTTGCTTAATTAAGA
CAGCTATGGAACAGCGGGATTCGCCAACCAACACGGTACGGAAAGTGACT
ACCGGAATACCTCGGTGTGGGAGAAGGGTAGAAATGTGGTGTGGGCCCCA
AACCTTGATTGTTACCATCAGCTTCACGCGTGGTTTGCTACAATGGAACG
GGTCCACTGAATGGCTATGGGTGTGGCAAAACTAGTAGTCGAAGTCGACA
ACGTGTCGGTCTGTACACCTGAATCAATAGCTTGCGGGGAAATTGAATAT
TTATGAGCACAGAAAACCGTACCTACTGCAACACAGGCCGGAAAGTCTTG
GTGGCCAACAACGGCCAACCACCTTTACCCCTGAAGCTTGCAACCCCCCA
CGCCTTCGACGACGACCGTTCCGTAGGCCCAAATGACCCAGGAGTTGAAG
CGGTTATCCAAGCGAAAGTTACGACTCTCATCTCCAGACGAGGCCCTGAG
CTTGAAGCCCTGCTGCTCTTACGGGACCCAGAACGTTCCGGGAACATCCC
GGTGAAGGCATTCTCGGAGTGCCTACGGGCGTGGGTTTCGAGCGACAGCG
AACGTGGCGGTGGCGGTGCCGCCGACCACACGCGGGCGGGCAAGCGTGAC
GGACCAAGCCAGGCGGCAGAGTTAACTCATGCCGACCGCAAGGTTTTGTA
CAGACGGTGGGCGGTGCGGGGACAGGTGAGGTACGTCGACTTCCTGGGCG
CGAGCGGGTATTTCAACGCCCCGCTGCCCCGCTACTCCGAGATCAGCACG
GCGGTCGCTGCCGCACCCGCTGCGTCTGCCGCTCCGGTGGTCCCCGACAA
GCTTGCACGGCACTCTGTCATCGGGAAACGGGCTTCCGGAAGGCTGCCGT
CCGGGGACACGACGGGCATGAATGCGAGGAGAGCTGATGCGGGGGAGGAG
GAGACGGGTCTCCTCGCAAGGGCGAGAGTCGTACTCGTACATCTATCGGA
GGTTTGATATCTGCGCGTGCTTTTTCCAAACCAGGGCTTGGGTGGATGAT
AAATTTCCCATAGCCTATATTATTGATGATCTCGGTACACCGTCCAAAGC
AGCAGCAGCAGCAGCAGCAGCGATGCCAACGGGATTTCTTGCGCGTTTCT
TATCCTAACCTCTTTCTTAAGTGGCCCAGACGGTATCGGACACAGGTCGG
ACCGGTTGTAAAGCTTTACGGAAACAGTCACAAGCTGCAGGTGCGAATGA
ACCGATGTCATGCGCTTTCCATGGACCGGCGCGTGTGGAACATACCGCAG
GTTTTGCGGGACGAAGGGGAGGAAGTATACTTTGCCGCATTCGAGGCGCA
CGACTACGGCAACTACCGGTGTCTAGATGAGGACGGCTTCGTAGATGCCA
TCGGAACCCTTGCTCCAGGTAATCATTGCCCGTCGATCTCCATGGCGCAG
CCGTACATACACTCTTTCCCTGCCGGCTTCAAACGTTGTGCCGTTGGAGG
CGGCATGTTGCCTGTGTGTTGTTCACAGATATTATGTTGCTGTAGCATCT
GCGTCGCGGTGGGGGGTAACATATTGCATTTCTGTCGAGAGGAGAGCACC
AGCACTGCTCCACACATCACGAGTCTGCGATTCTGAGTACGATCATCTCG
AGCAAAGTCTACTTCGATCATTCCCATCATGTTCCTCCCGGCGGGAGCTG
GGCGTAAACGAGCAGTGGGCTGATTCTAGGACATCTCCGTGCCCTTATCC
ACCGCCTGTCGGACCCCTGTTTGACAATTTTTTCTTCGCTCTCCGTGCTG
TCGATGCCGCGCAATCGAAAATTGGGCGCGGCCTTCGCGTACGCTTAGAG
GTGACGGCGGAGCAAGTGCACGACGTTTTCCTTGTGAGATCACGAATAGA
AGCAAGCGATGACAAGGCAAACTACGACGAGCTATTTAAGGTACATAAAC
ACAAGAAGGGACCGAAAGAGGAAGGAGGCTTGGCCTCTAGTCTAAGGATA
GAAATTGTGAGGGTGAAGGTGCACTTGGACTCTAAGGGTACCAAAAGGAC
GTTCCTGCCCTGGTCGGGAGAAACCTTCCGCCACAAAGGTATTTTCCACA
CCGAAGGTGGGAATGGTGGAGACTCAGCGTCGAGGGATAGCGGACAAAGT
AACTAAGAGAAACGAGGACTCGCTTGTGAGCCATGTAGGGCGAGGATTAA
CGACACCAGCACGAAGGTCAAGGATGGGCACAACCACGTGAAGAGAAAAC
GCAAAAGGGAGAAGGTTCGAACGGCGCTGGGACAGGGGCTCACACAAGCA
CACAAGCGGATAACGAAAGAAGAATAAGGTTATGCACCCGACCTAGTGGG
ACTGGCTTTTGAAGGGGCGTTTTGAGATGCGTAAATGGTGGCAGAGGCAC
GCTCGTCCTTGTTGGTGCGGGGAATCGTAGGTTATTACAATAATCTCTTC
GGGAGGGCACAAGTCATACCTTTTTCCATCTCCTGCCACTGGCGTCATCG
TGACTACGGCTCGCCCAAACATCATGCTAATGTACGCTTTCAACGCTCGT
ATCCACAAGACGGTGCACCATTCGTCGACTCCGCACAAGATAACTTGGTT
GCGTTTCCGCATGCCCCCCACCACGGCTATCCATTGGCGCCCACAAGGAC
TTGCGAAAGCTGGCTCAGATCGGAGGTTTCCTGTCGGGGGATGCTGTGGA
ACTCCGAGTTGAATCGCGGGGGGGTCGCGTGGACTGGGTCCCTGCCCAGG
TGAAGACGAGTTGATTCAGCCAGATCGGTAACCCTTGCTACGGGACTCTA
TTGATTCCAGCCGTGTGCGTTCAAACACCGACGAGACAAGCCAGCTCCTT
TCGAAGACAGCCACCATCAGAAATAGCAGAGAGAAGAGAACGGAAAACCA
TTGTCACTCGGAAATAGTGGTAAATGGAAATTTTACAAACAGGCGGTCGA
CAGTCTCGTCGTTGGCGTCGCCCCTCATCCGAGCCAAAAACGGTTCAGGC
TCACCCCCGCGACTGCTGTGACCGTCACACACCATGCATCACTGCTACGT
GGGTATGGGTGTTCCAGAGCGGAATAGTTCCCTATTGAACTCGTGATCTC
CAGATTGCTAGATCCGACGTGCGGAAGAGCCGAAGTGATACCTTCAATAT
CCTCGAAATCGCCCTACAACTGTTGAGTGGGCGAGATACATCGGAGAGAG
GTGTTCATCCAGAGAAGGGTATTCAAATCGTTCGAAGCGCGTGTCTAGGG
CGTCCATGCAGCGATCGTGGGGTTATTCTGTTCAGCCCCTCACAGGAAAC
TCCTCAAGAAGAATCGATTTCACGACCATCGGATCACCACACCAGCGGCA
CACGCCAACCTCAGTCGTACCTCCATTCCATTACCCTCCCTGTTTTCCCT
TTGCTCTGATTTAAGTGTAAGGCTCTTACCGATACTGAAAACAAGGTTAG
ACGAAATCAAGATCGAAACACCGCTAGTACTGTAGCCTCCGGGATGAGAG
CCTCTTTGATACAGAAGACGAAAGCGTTGAGTGTTGCATAGCACTAACAT
TGAACCGCCTCACTCGTGCGTGTTTCGGCGGGCACGTTTCACACCAGGTG
GTGTCAAGAGACGGGGATGGCACCTACACCGTTCGATTCAAGTCTCCGAC
AAAGGTCCCAGGCGCA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig14587:15..4380+

>mRNA_Ecto-sp13_S_contig14587.3517.1 ID=mRNA_Ecto-sp13_S_contig14587.3517.1|Name=mRNA_Ecto-sp13_S_contig14587.3517.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1179bp|location=Sequence derived from alignment at Ecto-sp13_S_contig14587:15..4380+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGGAGATTCGGCAAAGGTCCCGCCACGAGAGCGGGTGGGCTAGCATCCAC
GATGCCTGTGCAGGGTACATTGGGACAGCGCCCAAAACCGTTCTTCGCGA
CGGCTCTAATGCTGTCCCGATCGGACGACTCGCCTGCGTCGGAGGGAGGC
TCGCCTTTCGAAGGGTGGGGGGCGGGCAAAAGCCGTCGGGGCACAGGCAG
AACAGACTCCATCTTTACTCAAGGCCCAAATGACCCAGGAGTTGAAGCGG
TTATCCAAGCGAAAGTTACGACTCTCATCTCCAGACGAGGCCCTGAGCTT
GAAGCCCTGCTGCTCTTACGGGACCCAGAACGTTCCGGGAACATCCCGGT
GAAGGCATTCTCGGAGTGCCTACGGGCGTGGGTTTCGAGCGACAGCGAAC
GTGGCGGTGGCGGTGCCGCCGACCACACGCGGGCGGGCAAGCGTGACGGA
CCAAGCCAGGCGGCAGAGTTAACTCATGCCGACCGCAAGGTTTTGTACAG
ACGGTGGGCGGTGCGGGGACAGGTGAGGTACGTCGACTTCCTGGGCGCGA
GCGGGTATTTCAACGCCCCGCTGCCCCGCTACTCCGAGATCAGCACGGCG
GTCGCTGCCGCACCCGCTGCGTCTGCCGCTCCGGTGGTCCCCGACAAGCT
TGCACGGCACTCTGTCATCGGGAAACGGGCTTCCGGAAGGCTGCCGTCCG
GGGACACGACGGGCATGAATGCGAGGAGAGCTGATGCGGGGGAGGAGGAG
ACGGGTCTCCTCGCAAGGGCGAGAGTCGTACTCGTACATCTATCGGAGGT
TTTGCGGGACGAAGGGGAGGAAGTATACTTTGCCGCATTCGAGGCGCACG
ACTACGGCAACTACCGGTGTCTAGATGAGGACGGCTTCGTAGATGCCATC
GGAACCCTTGCTCCAGAGGTGACGGCGGAGCAAGTGCACGACGTTTTCCT
TGTGAGATCACGAATAGAAGCAAGCGATGACAAGGCAAACTACGACGAGC
TATTTAAGGACTTGCGAAAGCTGGCTCAGATCGGAGGTTTCCTGTCGGGG
GATGCTGTGGAACTCCGAGTTGAATCGCGGGGGGGTCGCGTGGACTGGGT
CCCTGCCCAGGTGGTGTCAAGAGACGGGGATGGCACCTACACCGTTCGAT
TCAAGTCTCCGACAAAGGTCCCAGGCGCA
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