mRNA_Ecto-sp13_S_contig805.19383.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig805.19383.1
Unique NamemRNA_Ecto-sp13_S_contig805.19383.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: D7FYG0_ECTSI (Glutamyl-tRNA(Gln) amidotransferase, B subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYG0_ECTSI)

HSP 1 Score: 151 bits (381), Expect = 4.970e-42
Identity = 77/92 (83.70%), Postives = 79/92 (85.87%), Query Frame = 2 
Query:  374 LLQKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAVGW--DPGDFCEWLIGDIAGSLNRCRNVSSVCRRMGAAVPVFRHS 643
            LLQKW SELCE PVGKRER Q EL LSEEDAAALSDE  VAFYFEEAVG   DPG+FC+WLIGDI GSLNR RNVSSVCRRMGAAVPVFR S
Sbjct:  139 LLQKWRSELCESPVGKRERYQSELGLSEEDAAALSDERSVAFYFEEAVGAGSDPGEFCKWLIGDITGSLNRWRNVSSVCRRMGAAVPVFRKS 230
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BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: D8LDP1_ECTSI (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDP1_ECTSI)

HSP 1 Score: 103 bits (258), Expect = 4.050e-22
Identity = 51/70 (72.86%), Postives = 56/70 (80.00%), Query Frame = 2 
Query:  374 LLQKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAVGW--DPGDFCEWLIGDIAGSLN 577
            LLQ+W SELCE P GKRER Q EL LSEEDA+ALSD+  VA YFEEAVG   DPG+ C+WLIGDIAG LN
Sbjct:  380 LLQRWRSELCELPAGKRERYQSELGLSEEDASALSDDQSVALYFEEAVGAGSDPGEACKWLIGDIAGYLN 449
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BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A6N2EEG6_9CYAN (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=3 Tax=Synechococcales TaxID=1890424 RepID=A0A6N2EEG6_9CYAN)

HSP 1 Score: 61.6 bits (148), Expect = 1.500e-7
Identity = 33/73 (45.21%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  374 LLQKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLNRCR 586
            L + W +EL E P  KR R   +L LS+ DA  L+DE P+A YFE  V  G DP     W+ GDIA  +N  R
Sbjct:  304 LREGWRAELPELPAAKRHRYAEQLGLSQYDARVLTDERPMADYFEAVVAAGADPKAAANWISGDIAAHVNANR 376
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BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: K9PB93_CYAGP (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=4 Tax=Synechococcales TaxID=1890424 RepID=K9PB93_CYAGP)

HSP 1 Score: 60.5 bits (145), Expect = 3.710e-7
Identity = 33/71 (46.48%), Postives = 39/71 (54.93%), Query Frame = 2 
Query:  380 QKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLNRCR 586
            + W +EL E P  KR R    L LS  DA  L+DE P+A YFE AV  G DP     W+ GDIA  +N  R
Sbjct:  310 ESWRAELPELPAAKRHRYAESLGLSIYDARVLTDERPMAEYFEAAVAAGGDPKAVANWVTGDIAAHVNANR 380
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A0G8ARX2_9SYNE (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=17 Tax=Synechococcus TaxID=1129 RepID=A0A0G8ARX2_9SYNE)

HSP 1 Score: 59.7 bits (143), Expect = 6.750e-7
Identity = 31/68 (45.59%), Postives = 40/68 (58.82%), Query Frame = 2 
Query:  380 QKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLN 577
            Q W +EL E P  +R     +L LSE DA  L+DE P+A YFE AV  G +P     WL+GD++  LN
Sbjct:  307 QAWQAELPELPAARRHCYGQQLGLSEYDARVLTDERPMADYFEAAVAAGGNPKTIANWLMGDLSAYLN 374
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A1S6YHX3_9EUKA (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=3 Tax=Paulinella micropora TaxID=1928728 RepID=A0A1S6YHX3_9EUKA)

HSP 1 Score: 59.7 bits (143), Expect = 6.750e-7
Identity = 32/66 (48.48%), Postives = 38/66 (57.58%), Query Frame = 2 
Query:  386 WHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLN 577
            W  EL E P  KR R   E+ LS  DA  L+DE P+A YFE A+  G DP     WL+GDI+  LN
Sbjct:  307 WICELPELPASKRHRYVEEIGLSIYDARVLTDELPMAEYFESAIASGADPKILANWLMGDISAYLN 372
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A7G8ERD6_9CYAN (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=2 Tax=unclassified Cyanobium TaxID=2627006 RepID=A0A7G8ERD6_9CYAN)

HSP 1 Score: 59.3 bits (142), Expect = 9.130e-7
Identity = 32/69 (46.38%), Postives = 38/69 (55.07%), Query Frame = 2 
Query:  386 WHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLNRCR 586
            W +EL E P  KR R    L LS+ DA  L+DE P+A YFE  V  G DP     W+ GDIA  +N  R
Sbjct:  312 WRAELPELPAAKRHRYADTLGLSQYDARVLTDERPMAAYFEAVVAAGADPKVTANWITGDIAAHVNANR 380
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A076HFM9_9SYNE (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=9 Tax=Bacteria TaxID=2 RepID=A0A076HFM9_9SYNE)

HSP 1 Score: 58.5 bits (140), Expect = 1.660e-6
Identity = 32/71 (45.07%), Postives = 39/71 (54.93%), Query Frame = 2 
Query:  380 QKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLNRCR 586
            + W +EL E P  KR R   EL LS+ DA  L+DE P+A YFE  V  G D      W+ GDIA  +N  R
Sbjct:  303 ESWRAELPELPAAKRHRYADELGLSQYDARVLTDERPMADYFEAVVAAGADAKLSANWITGDIAAHVNSNR 373
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: GATB_SYNSC (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B n=32 Tax=Synechococcales TaxID=1890424 RepID=GATB_SYNSC)

HSP 1 Score: 58.5 bits (140), Expect = 1.660e-6
Identity = 32/71 (45.07%), Postives = 39/71 (54.93%), Query Frame = 2 
Query:  380 QKWHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAVGW--DPGDFCEWLIGDIAGSLNRCR 586
            + W +EL E P  KR R   EL LS+ DA  L+DE P+A YFE  VG   D      W+ GDIA  +N  R
Sbjct:  306 ESWRAELPELPAAKRHRYADELGLSQYDARVLTDERPMADYFEAVVGAGADAKLAANWITGDIAAHVNSNR 376
[Close]
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Match: A0A2H4ZP99_9EUKA (Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=1 Tax=Paulinella longichromatophora TaxID=1708747 RepID=A0A2H4ZP99_9EUKA)

HSP 1 Score: 58.2 bits (139), Expect = 2.240e-6
Identity = 30/66 (45.45%), Postives = 39/66 (59.09%), Query Frame = 2 
Query:  386 WHSELCEFPVGKRERSQPELRLSEEDAAALSDEWPVAFYFEEAV--GWDPGDFCEWLIGDIAGSLN 577
            W+ EL E P  KR R   E+ LS  DA  ++DE P+A YFE A+  G +P     WL+GDI+  LN
Sbjct:  307 WNYELPELPASKRHRYVEEIGLSIYDARVITDELPMAEYFESAMASGAEPKALANWLMGDISAYLN 372
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig805.19383.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 21
Match NameE-valueIdentityDescription
D7FYG0_ECTSI4.970e-4283.70Glutamyl-tRNA(Gln) amidotransferase, B subunit n=1... [more]
D8LDP1_ECTSI4.050e-2272.86Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]
A0A6N2EEG6_9CYAN1.500e-745.21Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
K9PB93_CYAGP3.710e-746.48Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
A0A0G8ARX2_9SYNE6.750e-745.59Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
A0A1S6YHX3_9EUKA6.750e-748.48Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]
A0A7G8ERD6_9CYAN9.130e-746.38Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
A0A076HFM9_9SYNE1.660e-645.07Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
GATB_SYNSC1.660e-645.07Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase s... [more]
A0A2H4ZP99_9EUKA2.240e-645.45Glutamyl-tRNA(Gln) amidotransferase subunit B, mit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig805contigEcto-sp13_S_contig805:14782..17325 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score129.4
Seed ortholog evalue2e-27
Seed eggNOG ortholog2880.D7FYG0
Preferred nameGATB
Model size656
KEGG rclassRC00010
KEGG koko:K02434
KEGG ReactionR03905,R04212
KEGG Pathwayko00970,ko01100,map00970,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006082,GO:0006139,GO:0006399,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009987,GO:0016070,GO:0016874,GO:0016879,GO:0016884,GO:0019752,GO:0034641,GO:0034660,GO:0043038,GO:0043039,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044281,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046483,GO:0050567,GO:0070681,GO:0071704,GO:0090304,GO:0140098,GO:0140101,GO:1901360,GO:1901564
Exons3
EggNOG free text desc.Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln)
EggNOG OGsCOG0064@1,KOG2438@2759
EC6.3.5.6,6.3.5.7
Cds size654
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03029
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464078.1700926-CDS-Ecto-sp13_S_contig805:14782..150231681464078.1700926-CDS-Ecto-sp13_S_contig805:14782..15023Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig805 14783..15023 -
1681464078.1849859-CDS-Ecto-sp13_S_contig805:15536..156361681464078.1849859-CDS-Ecto-sp13_S_contig805:15536..15636Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig805 15537..15636 -
1681464078.1968143-CDS-Ecto-sp13_S_contig805:17012..173251681464078.1968143-CDS-Ecto-sp13_S_contig805:17012..17325Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig805 17013..17325 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig805.19383.1prot_Ecto-sp13_S_contig805.19383.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig805 14783..17325 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig805.19383.1

>prot_Ecto-sp13_S_contig805.19383.1 ID=prot_Ecto-sp13_S_contig805.19383.1|Name=mRNA_Ecto-sp13_S_contig805.19383.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=218bp
SARCSGFWACRTATGRTGSFVSTSTSSSGPRAKHASIPKYLAARKHTQSM
HARRVALLREVIRPTAPASKLLLEAVTIVALQEMELHLSSGGADWSGGRA
GTFEGEAGYRYFPEPDIPPLALHVVATEMAFRALRVSRRKEGAVPAGAPA
VGRGRCRSVGRVARGILLRGGGRVGPRGLLRVAHRGHRRFPQQVQKCFLR
VSQDGCSCSGVPPQFLRS
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mRNA from alignment at Ecto-sp13_S_contig805:14782..17325-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig805.19383.1 ID=mRNA_Ecto-sp13_S_contig805.19383.1|Name=mRNA_Ecto-sp13_S_contig805.19383.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2544bp|location=Sequence derived from alignment at Ecto-sp13_S_contig805:14782..17325- (Ectocarpus species13 EcNAP12_S_4_19m)
TCCGCCAGGTGCTCCGGTTTCTGGGCGTGCCGGACGGCGACAGGGCGGAC
GGGTTCATTCGTCTCCACGTCAACGTCAAGCTCAGGCCCAAGGGCGAAAC
ATGCCTCAATACCAAAGTATCTTGCTGCCAGAAAGCACACGCAGAGCATG
CACGCACGTCGTGTTGCACTATTGCGTGAGGTCATTCGTCCGACGGCCCC
AGCGAGTAAGTTGCTGTTGGAAGCGGTGACCATTGTAGCCCTTCAAGAAA
TGGAACTCCATCTATCATCAGGAGGGGCGGACTGGAGCGGTGGGCGGGCG
GGGACTTTCGAAGGTGAAACTGACGCATCAAAGCTGCAGCGGTTGTTAAC
GCCATCCGGTTCTTGTGAGGGAGTCGCGCTTTCAGGTAACTCAAGCAGGC
GCTGTAGACGGGGCGCAGCGGAAGCAGGCAGGTTTTTCCATCATAGCGAA
ACTGACTATCGTTGTCTTTAGCTAGATTGCCGGCGGTGCTGTTACCCTGC
TTGCTGCACTAAGTACGAGTGTTGAAGACGTTTGTCGCGGTTGTGAACCA
CCCCATGTTCGCTGTTTTGCAAGTGGAGGTCAAGAACCTCAACTTCTTCC
GAGCGGTGCAAGAGTCGGTGGACTTTGATATTATCGTGAGGCAGTCCTGG
TGCCTACTTTTATGTATGGTTTGGCTCCGCACAAAACAGGTACGACGATT
CTTCTACTCACGAGCACTCCGAAAAAGACATGCCGGCATATACCCGCTCT
TGCTTCAGCATCGGTTTCTCGCTGTTAAATGTTTTCTTCAACAAGCTGGG
CGTTTCGGCGAGGGTATACTGCCCGACACTAGATAGCTCCGTGCGGACCA
GCCAGCCTCACACACTCTTGAGTGGAAGCTGCTCTCCTGTGCACTAGCTG
TTCGATGTACGGAGTGCTAAGCGCAGGTCTGACAAGCCTGAGAAAACAGA
TCTTTGTTCCTCCATATCATTGTGTAGAACGAGTGCATGTAGCAACCGTA
GGGACGGAAATACATAGGTTGATTTTCTAGGTTTGAAACTCCACCTTTTG
TTTGTTTCATTCGGTACTGACTTTCCCTTGTCACAGGTGTCACGAGAAAG
ATGAGGTCATCCGGCAGGAGACGATATTGTGGGACGAGAAGAAGAGGGCA
ACCCAGATGATGCGCATCAAGGCGGGTCAGGCAGCCTGGTATTCGCACCA
GCAAGCATCATGGACAAGGTGCTCGTTTTGGGCCAAGGGTATCACCCGTG
CCCGAAAACATTTCGCTTGTTTTCAGTGTTCCGCAAGCGCCATCGACCAC
ACTTTGTCATAAAAAACAAACAAAAATATCACGTAAGTTTCTAGGTCGAA
AACAGTAGACCAGTCTTGTCTTGGCCTTCTTATTCGTACTGGTGTCCCTC
CGCACAATAATTGTCTCGGAAACTGTCACCAATGCCGTCTCGAGGGTTGT
TTGATTCGATGCCCCCGGAACGCTGAGCCTTCGCCTGTTAACGTTTGCAC
ACACGATTGAACGCCTCCAAGAGCCGTGGAACAACGACAGCAACCGCTCA
CGAACATTGACGCTCTTCAGTAGAGACACGAACACCGACGTTCTGTACTT
CAACCAGCACCATCCCGCCACCCCGTACATACCCTCCTTTCCACACGGAC
TCCACGCCCTCTCGTGCGGGTCAACTTGTGATCCTCCAGGCGAAGCGGGC
TATCGCTACTTCCCCGAGCCAGACATCCCACCGCTGGCGCTGCATGTAGT
TGCTACAGAAATGGCATTCCGAGCTCTGCGAGTTTCCCGGTACGTCCCTT
TCCTCGGACACGCAAGTGCAGCAGCGGGTGTTGTGTGGGCGCGTTGATGG
GGAAATGGGGCCGGATAGTCGTTGTCTAGGGTACAGATCTAAACCGGGCT
GTACGGTGATGCAAGCGTCTTGCCTGTGTGAACTCGAATCTGTACCTCCT
TCTCCGCCCCCCTCTCACGCCTCCGTTTGTGTTGCACACCGGATGGTAGG
GAGGCAATTAGCTCTGGCTGGCACAAGTGTATCCATGCTGGAACAAGCGG
ATTGCAGTTTTTTTGGTTCGCACGGAGTGTGGCAAACATCACGAAGGGGT
CCGTAATGTTCATGTTGGGAGTTTTCGTCAAGGCCTCGAGGGTGTTAAGG
CCGGTTCGAGCAAACAACGGAAGAGGTGAGCCGCCCATGCACCCACCACA
CAACGCTCCACTCCCTGTTCGCTACTACACCCATTCTCGTCATGTCCAAC
ATTCGGGTACTGGGGCGGGGGGAACGCGGGTTCTCTCACTGCTTCTCGAC
AGTCGGAAAGAGGGAGCGGTCCCAGCCGGAGCTCCGGCTGTCGGAAGAGG
ACGCTGCCGCTCTGTCGGACGAGTGGCCCGTGGCATTCTACTTCGAGGAG
GCGGTAGGGTGGGACCCCGGGGACTTTTGCGAGTGGCTCATCGGGGACAT
CGCCGGTTCCCTCAACAGGTGCAGAAATGTTTCCTCCGTGTGTCGCAGGA
TGGGTGCAGCTGTTCCGGTGTTCCGCCACAGTTTTTACGATCGC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig805:14782..17325-

>mRNA_Ecto-sp13_S_contig805.19383.1 ID=mRNA_Ecto-sp13_S_contig805.19383.1|Name=mRNA_Ecto-sp13_S_contig805.19383.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=654bp|location=Sequence derived from alignment at Ecto-sp13_S_contig805:14782..17325- (Ectocarpus species13 EcNAP12_S_4_19m)
TCCGCCAGGTGCTCCGGTTTCTGGGCGTGCCGGACGGCGACAGGGCGGAC
GGGTTCATTCGTCTCCACGTCAACGTCAAGCTCAGGCCCAAGGGCGAAAC
ATGCCTCAATACCAAAGTATCTTGCTGCCAGAAAGCACACGCAGAGCATG
CACGCACGTCGTGTTGCACTATTGCGTGAGGTCATTCGTCCGACGGCCCC
AGCGAGTAAGTTGCTGTTGGAAGCGGTGACCATTGTAGCCCTTCAAGAAA
TGGAACTCCATCTATCATCAGGAGGGGCGGACTGGAGCGGTGGGCGGGCG
GGGACTTTCGAAGGCGAAGCGGGCTATCGCTACTTCCCCGAGCCAGACAT
CCCACCGCTGGCGCTGCATGTAGTTGCTACAGAAATGGCATTCCGAGCTC
TGCGAGTTTCCCGTCGGAAAGAGGGAGCGGTCCCAGCCGGAGCTCCGGCT
GTCGGAAGAGGACGCTGCCGCTCTGTCGGACGAGTGGCCCGTGGCATTCT
ACTTCGAGGAGGCGGTAGGGTGGGACCCCGGGGACTTTTGCGAGTGGCTC
ATCGGGGACATCGCCGGTTCCCTCAACAGGTGCAGAAATGTTTCCTCCGT
GTGTCGCAGGATGGGTGCAGCTGTTCCGGTGTTCCGCCACAGTTTTTACG
ATCG
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