mRNA_Ecto-sp13_S_contig11707.1475.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11707.1475.1
Unique NamemRNA_Ecto-sp13_S_contig11707.1475.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: D7FHZ3_ECTSI (DNA-directed RNA polymerase subunit beta n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHZ3_ECTSI)

HSP 1 Score: 247 bits (630), Expect = 1.210e-73
Identity = 120/121 (99.17%), Postives = 121/121 (100.00%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV
Sbjct: 1014 KVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 1134
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A6H5JNN2_9PHAE (DNA-directed RNA polymerase subunit beta n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JNN2_9PHAE)

HSP 1 Score: 222 bits (565), Expect = 6.960e-67
Identity = 111/121 (91.74%), Postives = 114/121 (94.21%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMT+   + CL   VGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV
Sbjct:  394 KVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTMTAAV-CL---VGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 510
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A7S2HGK8_9STRA (DNA-directed RNA polymerase n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2HGK8_9STRA)

HSP 1 Score: 207 bits (526), Expect = 9.870e-65
Identity = 98/121 (80.99%), Postives = 108/121 (89.26%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRNVR+PQIGDKFASRHGQKGTIGMTYRQEDMP++ +GIVPDIIVNPHAIPSRMTIAQLIECLLGKV V  G EGDATPFTDVTV ++S  LH +G+QKHGNE MY GHTGR LN +V
Sbjct:   30 KVRIRNVRIPQIGDKFASRHGQKGTIGMTYRQEDMPFTAEGIVPDIIVNPHAIPSRMTIAQLIECLLGKVVVFQGCEGDATPFTDVTVEDISKRLHAMGYQKHGNEAMYQGHTGRPLNARV 150
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A7S3LGD2_9STRA (DNA-directed RNA polymerase n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3LGD2_9STRA)

HSP 1 Score: 207 bits (527), Expect = 9.110e-64
Identity = 96/121 (79.34%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRNVR+PQ+GDKFASRHGQKGTIGMTYRQEDMP++++GIVPDIIVNPHAIPSRMTIAQLIECLLGKV V  G EGDATPFTDVTV ++S+ LH +G+QKHGNE +Y GHTGR LN +V
Sbjct:  125 KVRIRNVRIPQVGDKFASRHGQKGTIGMTYRQEDMPFTVEGIVPDIIVNPHAIPSRMTIAQLIECLLGKVCVFQGCEGDATPFTDVTVEDISTRLHAMGYQKHGNEALYQGHTGRPLNARV 245
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A7S3Y3D8_HETAK (DNA-directed RNA polymerase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y3D8_HETAK)

HSP 1 Score: 206 bits (525), Expect = 1.470e-62
Identity = 96/121 (79.34%), Postives = 109/121 (90.08%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRN+RVPQIGDKFASRHGQKGTIGMTYRQEDMP++ +GI PD+IVNPHAIPSRMTI  L+ECLLGKV   TGTEGDATPFTDVTV N+S TLH++G+Q+HGNE M+SGHTGRML  KV
Sbjct:  207 KVRIRNLRVPQIGDKFASRHGQKGTIGMTYRQEDMPFTQEGISPDLIVNPHAIPSRMTIGHLVECLLGKVTSQTGTEGDATPFTDVTVENISDTLHRLGYQRHGNETMFSGHTGRMLKAKV 327
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A835YQ41_9STRA (DNA-directed RNA polymerase subunit beta n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQ41_9STRA)

HSP 1 Score: 213 bits (542), Expect = 9.930e-62
Identity = 99/121 (81.82%), Postives = 113/121 (93.39%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMP+S++GI PD+I+NPHAIPSRMTIA LIE L+GK G+L GTEGDATPFTDVTVA++SS LH++G+QKHGNE MYSGHTGRML +KV
Sbjct:  928 KVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPFSVEGITPDLIINPHAIPSRMTIAHLIETLMGKFGLLVGTEGDATPFTDVTVADISSRLHQMGYQKHGNETMYSGHTGRMLTSKV 1048
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A7S1UIJ8_9STRA (DNA-directed RNA polymerase n=2 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UIJ8_9STRA)

HSP 1 Score: 199 bits (505), Expect = 1.980e-61
Identity = 89/121 (73.55%), Postives = 107/121 (88.43%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VR+RN+RVPQIGDKF+SRHGQKGTIGMTYRQEDMPW+++G+VPDIIVNPHAIPSRMTI QL+ECL+GKV    G+E DAT F +VTV +VS  LHKIG+Q+HGNE +YSGHTGRM+  +V
Sbjct:   42 KVRVRNIRVPQIGDKFSSRHGQKGTIGMTYRQEDMPWTVEGVVPDIIVNPHAIPSRMTIGQLVECLMGKVSSKAGSEADATAFAEVTVDDVSKVLHKIGYQRHGNEAIYSGHTGRMICPRV 162
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A4P6JBU4_9LAMI (DNA-directed RNA polymerase (Fragment) n=10 Tax=Utricularia TaxID=13747 RepID=A0A4P6JBU4_9LAMI)

HSP 1 Score: 192 bits (488), Expect = 2.280e-60
Identity = 87/121 (71.90%), Postives = 102/121 (84.30%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VR+R+VR+PQIGDKF+SRHGQKGTIGMTY QEDMPW+++GI PDIIVNPHAIPSRMTI QLIEC++GKV    G EGDATPFTDVTV N+S  LHK G+Q  G E MY+GHTGR L+  +
Sbjct:   48 KVRVRSVRIPQIGDKFSSRHGQKGTIGMTYTQEDMPWTVEGITPDIIVNPHAIPSRMTIGQLIECVMGKVAAHMGKEGDATPFTDVTVENISKALHKCGYQMRGFETMYNGHTGRRLSAMI 168
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: Q70NR8_ERYGU (DNA-directed RNA polymerase (Fragment) n=1 Tax=Erythranthe guttata TaxID=4155 RepID=Q70NR8_ERYGU)

HSP 1 Score: 191 bits (484), Expect = 6.510e-60
Identity = 87/121 (71.90%), Postives = 101/121 (83.47%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            ++R+R+VR+PQIGDKF+SRHGQKGTIGMTY QEDMPWSI+GI PDIIVNPHAIPSRMTI QLIEC++GKV    G EGDATPFTDVTV ++S  LHK G+Q  G E MY+GHTGR L   +
Sbjct:   49 KIRMRSVRIPQIGDKFSSRHGQKGTIGMTYTQEDMPWSIEGITPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDSISKALHKCGYQMRGFETMYNGHTGRQLTAMI 169
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BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Match: A0A0D2ZXJ9_BRAOL (DNA-directed RNA polymerase n=1 Tax=Brassica oleracea var. oleracea TaxID=109376 RepID=A0A0D2ZXJ9_BRAOL)

HSP 1 Score: 189 bits (481), Expect = 7.820e-60
Identity = 86/121 (71.07%), Postives = 101/121 (83.47%), Query Frame = 1 
Query:    1 QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNPHAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGFQKHGNEVMYSGHTGRMLNTKV 363
            +VR+R+VR+PQIGDKF+SRHGQKGT+GMTY QEDMPW+I+G+ PDIIVNPHAIPSRMTI QLIEC++GKV    G EGDATPFTDVTV N+S  LHK G+Q  G E MY+GHTGR L   +
Sbjct:   18 KVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTIEGVTPDIIVNPHAIPSRMTIGQLIECIMGKVAAQMGKEGDATPFTDVTVDNISKALHKCGYQMRGFERMYNGHTGRPLPAMI 138
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11707.1475.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FHZ3_ECTSI1.210e-7399.17DNA-directed RNA polymerase subunit beta n=1 Tax=E... [more]
A0A6H5JNN2_9PHAE6.960e-6791.74DNA-directed RNA polymerase subunit beta n=1 Tax=E... [more]
A0A7S2HGK8_9STRA9.870e-6580.99DNA-directed RNA polymerase n=1 Tax=Helicotheca ta... [more]
A0A7S3LGD2_9STRA9.110e-6479.34DNA-directed RNA polymerase n=1 Tax=Amphora coffei... [more]
A0A7S3Y3D8_HETAK1.470e-6279.34DNA-directed RNA polymerase n=1 Tax=Heterosigma ak... [more]
A0A835YQ41_9STRA9.930e-6281.82DNA-directed RNA polymerase subunit beta n=1 Tax=T... [more]
A0A7S1UIJ8_9STRA1.980e-6173.55DNA-directed RNA polymerase n=2 Tax=Phaeomonas par... [more]
A0A4P6JBU4_9LAMI2.280e-6071.90DNA-directed RNA polymerase (Fragment) n=10 Tax=Ut... [more]
Q70NR8_ERYGU6.510e-6071.90DNA-directed RNA polymerase (Fragment) n=1 Tax=Ery... [more]
A0A0D2ZXJ9_BRAOL7.820e-6071.07DNA-directed RNA polymerase n=1 Tax=Brassica olera... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11707contigEcto-sp13_S_contig11707:3571..4638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score248.1
Seed ortholog evalue2.2e-63
Seed eggNOG ortholog2880.D7FHZ3
Preferred nameRPB2
Model size363
KEGG rclassRC02795
KEGG koko:K03010
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko05016,ko05169,map00230,map00240,map01100,map03020,map05016,map05169
KEGG ModuleM00180
Hectar predicted targeting categoryother localisation
GOsGO:0000428,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003899,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0005737,GO:0005829,GO:0005911,GO:0006139,GO:0006351,GO:0006366,GO:0006396,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009506,GO:0009892,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0010494,GO:0010605,GO:0010608,GO:0010629,GO:0014070,GO:0016070,GO:0016441,GO:0016458,GO:0016591,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019222,GO:0019438,GO:0030054,GO:0030880,GO:0031047,GO:0031050,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0034062,GO:0034641,GO:0034645,GO:0034654,GO:0035194,GO:0035195,GO:0035196,GO:0035770,GO:0036464,GO:0040029,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043331,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0048519,GO:0050789,GO:0050896,GO:0051716,GO:0055029,GO:0055044,GO:0060255,GO:0061695,GO:0065007,GO:0070013,GO:0070887,GO:0070918,GO:0071310,GO:0071359,GO:0071407,GO:0071704,GO:0090304,GO:0097159,GO:0097659,GO:0097747,GO:0140098,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1901698,GO:1901699,GO:1902494,GO:1990234,GO:1990904
Exons2
EggNOG free text desc.DNA-directed 5'-3' RNA polymerase activity
EggNOG OGsCOG0085@1,KOG0214@2759
EC2.7.7.6
Cds size363
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01611,ko00000,ko00001,ko00002,ko01000,ko03021,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462616.4777658-CDS-Ecto-sp13_S_contig11707:3570..37261681462616.4777658-CDS-Ecto-sp13_S_contig11707:3570..3726Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11707 3571..3726 -
1681462616.499709-CDS-Ecto-sp13_S_contig11707:4431..46381681462616.499709-CDS-Ecto-sp13_S_contig11707:4431..4638Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11707 4432..4638 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11707.1475.1prot_Ecto-sp13_S_contig11707.1475.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11707 3571..4638 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11707.1475.1

>prot_Ecto-sp13_S_contig11707.1475.1 ID=prot_Ecto-sp13_S_contig11707.1475.1|Name=mRNA_Ecto-sp13_S_contig11707.1475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=121bp
QVRIRNVRVPQIGDKFASRHGQKGTIGMTYRQEDMPWSIDGIVPDIIVNP
HAIPSRMTIAQLIECLLGKVGVLTGTEGDATPFTDVTVANVSSTLHKIGF
QKHGNEVMYSGHTGRMLNTKV
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mRNA from alignment at Ecto-sp13_S_contig11707:3571..4638-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11707.1475.1 ID=mRNA_Ecto-sp13_S_contig11707.1475.1|Name=mRNA_Ecto-sp13_S_contig11707.1475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1068bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11707:3571..4638- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGTGCGCATCCGTAACGTGCGCGTCCCGCAGATCGGCGACAAGTTCGC
CTCCCGGCACGGGCAGAAGGGTACCATCGGGATGACCTACCGGCAAGAGG
ACATGCCCTGGTCGATCGACGGCATCGTTCCCGACATCATCGTTAACCCG
CACGCCATCCCCAGCCGGATGACCATCGCGCAGCTGATCGAGTGTCTTCT
CGGAAAGGTGAGCTTAAGGGGGGCGGTGGTCGAGAATTGCTTTATTTGGG
GGGGGGGGGGATTGCGTGTTCCCACTCTGTGGGGCCGTTCCCGGTATTTT
TTTTGTTGCAGAGTTGCGAGTGTCGTTTTCCATTGAATTGTTGCCGTCGG
CGGTTACGCTTTTTTTCTTGGGATGTGCTGGAAATTTCCACGCTGGGGGA
CAGCTGAAGCGAAGAGAAGTGCTTGTAGTAGTTAATATAAATTCTTGAAC
AGCGGTGTCGGTCAGTTGTGGTTGATGCGGCCTTCTTGTTTGTGTGTTGT
TGTTGTGTTGCTGTTGGCTCTTCGGTTCTTTGGCTTGCACCCTCCTCCCT
GTCTCTCGTTTTCTCACGGGCGAGGTGACGTTTCTTGGCTGAAAAGTTGT
CGAGTGCTACGTGTGACTCTTTTTCAGGTGTGGCAATCGATATGCAACCA
TATCCCGCATCCGCCCGTGCTTTCATCGTCAGATGGTGATCACGATAGTC
CTCGACCGAGTTATGCTGCCGTTGACTCTGAGCCATTATCCGATGGAAGC
CTCCGTGGTTGTAATTATTATCTTGCGTCTTGTTTGTTTAACTTGCACGT
TTTTAGGTGTGCATCTTGTGTCGTTGTTGTTTTGCCAGTTTTTTATTCGT
TGCTTTTCTCTAGTTTTTGTTCATGTTTTATTTCTTTCCTGTCCGTTGAT
TGGTCGAAACAGGTGGGCGTGCTGACGGGAACGGAAGGCGACGCCACGCC
CTTCACGGACGTGACCGTGGCGAACGTTAGCAGCACCCTGCACAAGATCG
GCTTCCAGAAGCACGGCAACGAGGTCATGTACAGCGGCCACACTGGCCGC
ATGCTCAACACGAAGGTT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11707:3571..4638-

>mRNA_Ecto-sp13_S_contig11707.1475.1 ID=mRNA_Ecto-sp13_S_contig11707.1475.1|Name=mRNA_Ecto-sp13_S_contig11707.1475.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=363bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11707:3571..4638- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGTGCGCATCCGTAACGTGCGCGTCCCGCAGATCGGCGACAAGTTCGC
CTCCCGGCACGGGCAGAAGGGTACCATCGGGATGACCTACCGGCAAGAGG
ACATGCCCTGGTCGATCGACGGCATCGTTCCCGACATCATCGTTAACCCG
CACGCCATCCCCAGCCGGATGACCATCGCGCAGCTGATCGAGTGTCTTCT
CGGAAAGGTGGGCGTGCTGACGGGAACGGAAGGCGACGCCACGCCCTTCA
CGGACGTGACCGTGGCGAACGTTAGCAGCACCCTGCACAAGATCGGCTTC
CAGAAGCACGGCAACGAGGTCATGTACAGCGGCCACACTGGCCGCATGCT
CAACACGAAGGTT
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