mRNA_E-siliculosus-1a_F_contig10178.170.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Homology

BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: D7FRH8_ECTSI (Signal peptide peptidase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FRH8_ECTSI)

HSP 1 Score: 216 bits (550), Expect = 8.440e-64
Identity = 107/110 (97.27%), Postives = 108/110 (98.18%), Query Frame = 1

Query:    1 RFSYRSWGGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEV 330
            RFSYRSWGGGYLACV+EVRDAVTRFRE GSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGL GSGGQKLFFRGFLDKYGIKPEV
Sbjct:   25 RFSYRSWGGGYLACVQEVRDAVTRFREAGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLTGSGGQKLFFRGFLDKYGIKPEV 134

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A372JBQ3_9ACTN (Signal peptide peptidase SppA n=3 Tax=Actinomadura TaxID=1988 RepID=A0A372JBQ3_9ACTN)

HSP 1 Score: 74.7 bits (182), Expect = 2.400e-12
Identity = 42/105 (40.00%), Postives = 58/105 (55.24%), Query Frame = 1

Query:   22 GGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVVR 336
            GG  LA V+EVR+AV   R  G                   ++T+A A+TFGEGG     Y+LASAF++V +Q TG VGL G   ++ FF G L+K G++P   +
Sbjct:   77 GGAGLALVQEVREAVGALRAAG-------------------KYTVAWAETFGEGGRGTVPYYLASAFDRVFLQPTGEVGLTGVALEEPFFAGALEKAGVQPRFAK 162

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A7S0YTA2_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0YTA2_9CRYP)

HSP 1 Score: 70.1 bits (170), Expect = 8.570e-11
Identity = 37/100 (37.00%), Postives = 57/100 (57.00%), Query Frame = 1

Query:   34 LACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVV 333
            +A ++E+RDAV  FR      SA  GE       + +R  +  ADTFGE G   G+Y+LA+AF+K+ +Q +G VG+ G   +  F +  L K G+ P+ +
Sbjct:   93 MATMQEIRDAVMAFR------SAQGGE-------EARRPAVYAADTFGESGQGTGQYYLATAFDKIFIQPSGLVGIIGLHSEAFFLKRMLAKLGVHPQFI 179

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A5Q4DG26_9BACT (Signal peptide peptidase SppA n=1 Tax=Gemmatimonadales bacterium TaxID=2448054 RepID=A0A5Q4DG26_9BACT)

HSP 1 Score: 65.5 bits (158), Expect = 3.800e-9
Identity = 41/108 (37.96%), Postives = 57/108 (52.78%), Query Frame = 1

Query:   22 GGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVVRSRG 345
            G G  A  +E+  AVTRFR  G                     T+  A+TFGE  PA G YFLA+AF+++++Q +G VGL G   +  FFRG  +++ I P+   SRG
Sbjct:   97 GVGGWATTQEIHGAVTRFRASGKP-------------------TLLFAETFGELAPAQGAYFLATAFDEIVLQPSGEVGLAGLALEAPFFRGVFERWDIVPQF-ESRG 184

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A545TXP2_9PROT (Protease 4 n=1 Tax=Denitrobaculum tricleocarpae TaxID=2591009 RepID=A0A545TXP2_9PROT)

HSP 1 Score: 64.3 bits (155), Expect = 9.400e-9
Identity = 34/97 (35.05%), Postives = 55/97 (56.70%), Query Frame = 1

Query:   34 LACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKP 324
            ++ V+E+R+A+  FR  G                   +F+IA A++FGEGG     Y+LASAFE++ +Q +G + + G   +  + RG LDK+G+KP
Sbjct:  111 ISQVQELREAIAGFRATG-------------------KFSIAFAESFGEGGDGTLHYYLASAFEEIWLQPSGDLDITGIQLESPYIRGLLDKHGVKP 188

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A2E2QB37_9PROT (Protease 4 n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A2E2QB37_9PROT)

HSP 1 Score: 63.9 bits (154), Expect = 1.290e-8
Identity = 36/105 (34.29%), Postives = 56/105 (53.33%), Query Frame = 1

Query:   25 GGY-LACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVVR 336
            G Y LA + +++ A+ +FR  G                   ++T A AD+FGE G  + EYFLA++F++V +Q  G + LNG     L+F+G  DKY + PE  +
Sbjct:  126 GNYSLAQLYDLKAAIKKFRGAG-------------------KYTYAYADSFGEAGYGMDEYFLAASFDEVWMQPLGSLSLNGFALSNLYFKGLFDKYKVMPEFFQ 211

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A7K0BPV9_9ACTN (Protease 4 n=1 Tax=Actinomadura macrotermitis TaxID=2585200 RepID=A0A7K0BPV9_9ACTN)

HSP 1 Score: 63.2 bits (152), Expect = 2.320e-8
Identity = 36/105 (34.29%), Postives = 55/105 (52.38%), Query Frame = 1

Query:   22 GGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVVR 336
            GG  LA  +E+R AV   R+ G                   ++T+A A+TFGEGG     Y+LA+ F+K+ +Q +G VGL G   ++ F  G L+K G++P   +
Sbjct:   77 GGVGLAMTQELRAAVQAVRDAG-------------------KYTVAWAETFGEGGRGSVPYYLATGFDKIWLQPSGEVGLTGVALEEPFLAGALEKAGVQPRFAK 162

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: UPI000408AB31 (signal peptide peptidase SppA n=1 Tax=Geminicoccus roseus TaxID=404900 RepID=UPI000408AB31)

HSP 1 Score: 62.0 bits (149), Expect = 5.790e-8
Identity = 36/103 (34.95%), Postives = 53/103 (51.46%), Query Frame = 1

Query:   34 LACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEVVRSR 342
             A  +E+R A+   R  G                   + T+A AD+FGE  P    Y+LASAFE++++Q  G VGL G   ++ F RG +DK G++ EV + R
Sbjct:  102 FATTQELRQAILAVRAAG-------------------KTTLAFADSFGELSPGNEGYYLASAFEQIVLQPVGSVGLTGLAMEEPFARGLIDKLGVELEVTKRR 185

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A7V9AHG6_9BACT (Signal peptide peptidase SppA n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A7V9AHG6_9BACT)

HSP 1 Score: 62.0 bits (149), Expect = 5.800e-8
Identity = 36/103 (34.95%), Postives = 53/103 (51.46%), Query Frame = 1

Query:   22 GGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEV 330
            GG  L  + E+RDAVT FR  G  A                   I  ++TFG+GGP  G Y+LA+AF+ + +Q +G + + G   +  F  G LD+ GI+P +
Sbjct:   85 GGLGLGQIEEIRDAVTAFRASGKPA-------------------ILFSETFGDGGPGHGGYYLATAFDSIFLQPSGDLSVAGLRIEVPFVAGTLDRVGIEPRI 168

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BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Match: A0A368T4M0_9ACTN (Signal peptide peptidase SppA n=1 Tax=Marinitenerispora sediminis TaxID=1931232 RepID=A0A368T4M0_9ACTN)

HSP 1 Score: 62.0 bits (149), Expect = 5.800e-8
Identity = 37/96 (38.54%), Postives = 52/96 (54.17%), Query Frame = 1

Query:   43 VREVRDAVTRFREVGSDASATAGEGAEADGRQRKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGFLDKYGIKPEV 330
            V+E+R AVT FR  G                     T+A +++FGE GP    Y+LA+AFE++++Q +G VGL G      FFR  +DK GI+ EV
Sbjct:   85 VQELRAAVTAFRRSGKP-------------------TVAWSESFGEFGPGTVPYYLAAAFEEIVLQPSGSVGLTGLSVTNRFFRDAVDKLGIEYEV 161

The following BLAST results are available for this feature:

BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot

BLAST of mRNA_E-siliculosus-1a_F_contig10178.170.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25

ZOOM

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POSITION

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Match Name	E-value	Identity	Description
D7FRH8_ECTSI	8.440e-64	97.27	Signal peptide peptidase n=2 Tax=Ectocarpus TaxID=...	[more]
A0A372JBQ3_9ACTN	2.400e-12	40.00	Signal peptide peptidase SppA n=3 Tax=Actinomadura...	[more]
A0A7S0YTA2_9CRYP	8.570e-11	37.00	Hypothetical protein (Fragment) n=1 Tax=Hemiselmis...	[more]
A0A5Q4DG26_9BACT	3.800e-9	37.96	Signal peptide peptidase SppA n=1 Tax=Gemmatimonad...	[more]
A0A545TXP2_9PROT	9.400e-9	35.05	Protease 4 n=1 Tax=Denitrobaculum tricleocarpae Ta...	[more]
A0A2E2QB37_9PROT	1.290e-8	34.29	Protease 4 n=2 Tax=Alphaproteobacteria TaxID=28211...	[more]
A0A7K0BPV9_9ACTN	2.320e-8	34.29	Protease 4 n=1 Tax=Actinomadura macrotermitis TaxI...	[more]
UPI000408AB31	5.790e-8	34.95	signal peptide peptidase SppA n=1 Tax=Geminicoccus...	[more]
A0A7V9AHG6_9BACT	5.800e-8	34.95	Signal peptide peptidase SppA n=1 Tax=Gemmatimonad...	[more]
A0A368T4M0_9ACTN	5.800e-8	38.54	Signal peptide peptidase SppA n=1 Tax=Mariniteneri...	[more]

Pages

Properties


Property Name	Value
Start	0
Stop	1
Cds size	387
Model size	556
Exons	4
Hectar predicted targeting category	other localisation
Best eggNOG OG	NA\|NA\|NA
Best tax level	Eukaryota
COG Functional cat.	OU
EggNOG OGs	2QQH5@2759,COG0616@1
EggNOG free text desc.	signal peptide processing
Seed eggNOG ortholog	2880.D7FRH8
Seed ortholog evalue	2.6e-54
Seed ortholog score	218.0
Taxonomic scope	Eukaryota

Relationships

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E-siliculosus-1a_F_contig10178.170.1	prot_E-siliculosus-1a_F_contig10178.170.1	Ectocarpus siliculosus Ec863f_EcPH12_90f female	polypeptide	E-siliculosus-1a_F_contig10178 1355..2453 -

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622927519.1073864-UTR-E-siliculosus-1a_F_contig10178:641..732	1622927519.1073864-UTR-E-siliculosus-1a_F_contig10178:641..732	Ectocarpus siliculosus Ec863f_EcPH12_90f female	UTR	E-siliculosus-1a_F_contig10178 642..732 -
1696318564.9436283-UTR-E-siliculosus-1a_F_contig10178:641..732	1696318564.9436283-UTR-E-siliculosus-1a_F_contig10178:641..732	Ectocarpus siliculosus Ec863f_EcPH12_90f female	UTR	E-siliculosus-1a_F_contig10178 642..732 -
1622927519.1182497-UTR-E-siliculosus-1a_F_contig10178:1276..1354	1622927519.1182497-UTR-E-siliculosus-1a_F_contig10178:1276..1354	Ectocarpus siliculosus Ec863f_EcPH12_90f female	UTR	E-siliculosus-1a_F_contig10178 1277..1354 -
1696318564.954128-UTR-E-siliculosus-1a_F_contig10178:1276..1354	1696318564.954128-UTR-E-siliculosus-1a_F_contig10178:1276..1354	Ectocarpus siliculosus Ec863f_EcPH12_90f female	UTR	E-siliculosus-1a_F_contig10178 1277..1354 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622927519.1287785-CDS-E-siliculosus-1a_F_contig10178:1354..1398	1622927519.1287785-CDS-E-siliculosus-1a_F_contig10178:1354..1398	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 1355..1398 -
1696318564.9659095-CDS-E-siliculosus-1a_F_contig10178:1354..1398	1696318564.9659095-CDS-E-siliculosus-1a_F_contig10178:1354..1398	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 1355..1398 -
1622927519.1442537-CDS-E-siliculosus-1a_F_contig10178:1797..1951	1622927519.1442537-CDS-E-siliculosus-1a_F_contig10178:1797..1951	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 1798..1951 -
1696318564.9741724-CDS-E-siliculosus-1a_F_contig10178:1797..1951	1696318564.9741724-CDS-E-siliculosus-1a_F_contig10178:1797..1951	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 1798..1951 -
1622927519.1561193-CDS-E-siliculosus-1a_F_contig10178:2264..2453	1622927519.1561193-CDS-E-siliculosus-1a_F_contig10178:2264..2453	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 2265..2453 -
1696318564.9823525-CDS-E-siliculosus-1a_F_contig10178:2264..2453	1696318564.9823525-CDS-E-siliculosus-1a_F_contig10178:2264..2453	Ectocarpus siliculosus Ec863f_EcPH12_90f female	CDS	E-siliculosus-1a_F_contig10178 2265..2453 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E-siliculosus-1a_F_contig10178:642..2453-
coding sequence from alignment at E-siliculosus-1a_F_contig10178:642..2453-

protein sequence of mRNA_E-siliculosus-1a_F_contig10178.170.1

>prot_E-siliculosus-1a_F_contig10178.170.1 ID=prot_E-siliculosus-1a_F_contig10178.170.1|Name=mRNA_E-siliculosus-1a_F_contig10178.170.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=129bp
RFSYRSWGGGYLACVREVRDAVTRFREVGSDASATAGEGAEADGRQRKRF
TIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLNGSGGQKLFFRGF
LDKYGIKPEVVRSRGVQERRGESGPEGV*

mRNA from alignment at E-siliculosus-1a_F_contig10178:642..2453-

Legend: polypeptideCDSUTR

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E-siliculosus-1a_F_contig10178.170.1 ID=mRNA_E-siliculosus-1a_F_contig10178.170.1|Name=mRNA_E-siliculosus-1a_F_contig10178.170.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1812bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10178:642..2453- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
AGGTTCAGCTACCGCAGCTGGGGAGGGGGGTACTTGGCTTGCGTTCGAGA
GGTCCGCGATGCCGTCACTCGCTTCCGCGAGGTCGGCAGCGATGCTTCTG
CCACCGCTGGTGAAGGAGCAGAGGCGGACGGGCGGCAGCGAAAGAGGTTT
ACTATCGCGGTAGCAGATACGTTCGGGGAAGGTGGTCCAGTGAGATGGCT
GGGTAGGACTGGTTTGTCGGTTCGACGCGTCGCAGTTGTCAGGAGGATGA
TGCTGTGATCACGATCTCGAGACACATTGTGCTCGATTGCCATGCGTCTA
GCTCCTACGTTCGTCGGGTTGTTAGTGAAGTGTTTGAATTTTCAGGTGGA
AACAGGGACTATTGCCCCTGCGTGAGACAAGTTGCTGCTAGATTCCGAGG
TCGAATTCAGTTGACTCGTACGCGGGGTCTCCTGCTTTACTATGGCCGCC
CAGGTTCTGACTCGACCGGAATTATCATGTTCGGGGTTTCCTTTCCCAAC
AGGCCGTTGGCGAGTACTTCTTGGCCTCCGCATTCGAGAAGGTCCTGGTG
CAAAAGACTGGCTACGTGGGTCTTAACGGGAGCGGTGGCCAAAAGCTGTT
TTTCAGGGGCTTCTTGGACAAGTACGGCATTAAGCCCGAGGTCGTTCGCT
CGCGAGGTGGGTACGAAAACTTTACATTGCACGTGTGGCATATGTCGCCT
TGCGATACGACTTGTCCTCTAAAAACCCAAGATATAATTGTTTAATATGG
TACATAAAAACCCGTAGGTTAGATTTGTGTCTTCTTCGACCGCCCCAAGA
TAGCGAAAAGTGTCCAGGATTGTCTCGTTGTACAATGGCCACTAGATAGG
GACAAGGTTGACTTTGAAGTCGATTTCGGATCAGTTTCTCAAGAGGTGGG
CATGCTGCATGAAACTATCGTGGCAAGGTCGGGCGTTTACTTACATCTAA
ATCAATTCGTGCTGAATTCAGAAGGGCAATTGTGTCGGGTTTGAAGGGGC
ATTCGATGGTTGACGCTCGACCACCTCCTGAAGCGTTGATGCACATTCCA
CACAGGAGTACAAGAGCGCCGCGGAGAGTCTGGTCCGGAAGGCGTATAGC
CCGCACGAGAGAGAAGCGATGACGTCCCTTCTCACGTCCATGCTCGACGA
CGTTGCAGACGGTGTCGCGGCTTCCCGGTGAGGCCTGGGGTTAGACTTCT
GCAGCAGCCGCTGCTGCATCTTTGCTGTTGGACGCTACTCCTCTGACGAG
GGGCTAACCCTAACCTAACCCTAACGTTCCTAGGGATACATACGTGAGAA
GTAACCGGTGGTCACAAAACGTGCACTTTGTTTTTCTTGACGAGAATATT
GCACCTCTGATCTAGGTACATAGTCTAGTCCCTTTCCTTCTGAAGGGGGA
ATAATAGTGATGACCACCTCCGTTTGCTTCCGAGTGCTACCAACCACTGT
GGATGTCGGTATCAACATTGCGGTGACGAGTTCTCAGCAGCAGCAGTAGA
ATATATAGGGCAGATGCTGACGTCTGACGTTGTACATCTGGATCATGGCA
CTGTATGGCTTGGTCGAGATCGGGGAGGTGGACAGATGGGGTTGCATGAG
AGAGTTAACGATACTATTAATATTGCTCTGCACAGCACGTGAACTGCTCT
CGAAGTGCAAGGTTGTTTGCGTTTCGTTCAGTTTTTTTCCCTGCTGTATG
CATATCACCGTCCTGAACCAGGGGTTTCAAGGACCGGAAGGATGTGTACG
CCATCATGCACGACTGTCCCCTCCCGGCGACGCTTGCCCTGTCGGCTAAC
CTCATCGACGGT

Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig10178:642..2453-

>mRNA_E-siliculosus-1a_F_contig10178.170.1 ID=mRNA_E-siliculosus-1a_F_contig10178.170.1|Name=mRNA_E-siliculosus-1a_F_contig10178.170.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=774bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10178:642..2453- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
AGGTTCAGCTACCGCAGCTGGGGAGGGGGGTACTTGGCTTGCGTTCGAGA
GGTCCGCGATGCCGTCACTCGCTTCCGCGAGGTCGGCAGCGATGCTTCTG
CCACCGCTGGTGAAGGAGCAGAGGCGGACGGGCGGCAGCGAAAGAGGTTT
ACTATCGCGGTAGCAGATACGTTCGGGGAAGGTGGTCCAAGGTTCAGCTA
CCGCAGCTGGGGAGGGGGGTACTTGGCTTGCGTTCGAGAGGTCCGCGATG
CCGTCACTCGCTTCCGCGAGGTCGGCAGCGATGCTTCTGCCACCGCTGGT
GAAGGAGCAGAGGCGGACGGGCGGCAGCGAAAGAGGTTTACTATCGCGGT
AGCAGATACGTTCGGGGAAGGTGGTCCAGCCGTTGGCGAGTACTTCTTGG
CCTCCGCATTCGAGAAGGTCCTGGTGCAAAAGACTGGCTACGTGGGTCTT
AACGGGAGCGGTGGCCAAAAGCTGTTTTTCAGGGGCTTCTTGGACAAGTA
CGGCATTAAGCCCGAGGTCGTTCGCTCGCGAGGCCGTTGGCGAGTACTTC
TTGGCCTCCGCATTCGAGAAGGTCCTGGTGCAAAAGACTGGCTACGTGGG
TCTTAACGGGAGCGGTGGCCAAAAGCTGTTTTTCAGGGGCTTCTTGGACA
AGTACGGCATTAAGCCCGAGGTCGTTCGCTCGCGAGGAGTACAAGAGCGC
CGCGGAGAGTCTGGTCCGGAAGGCGTATAGGAGTACAAGAGCGCCGCGGA
GAGTCTGGTCCGGAAGGCGTATAG