mRNA_E-fasciculatus_F_contig16072.4155.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig16072.4155.1
Unique NamemRNA_E-fasciculatus_F_contig16072.4155.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig16072.4155.1 vs. uniprot
Match: D8LPM9_ECTSI (BRCT domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LPM9_ECTSI)

HSP 1 Score: 356 bits (914), Expect = 1.300e-108
Identity = 439/561 (78.25%), Postives = 447/561 (79.68%), Query Frame = 1 
Query:    1 MDDIGVVDDGGGEADTPTTRPVSRLAPRNGPSLGQTSGSGGRQLAPVAEGRPVSLAAAAAAPAALETAEALLSMSPSVRHGGGFLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--ACGDPVGTPNSTASSSRRLGPGSPSSATDLXXXXXXXXXXXXXSYRSPALARPPNASXXXXXXXXXXXXXXXXXXXXXXXXXXXXHTARRPATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSLSRVRRTSRKGWCSPDLAAGQANAACLSYLLGETQHPELDLDPDLGELLDPDEGSSGAGSAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTNRGDKG------RNRPQASDGVEGGPSQGQGRXXXXXXXXXXS---ESTEGMNVFDISALSAGSNSGDSGM 1650
            MDD+GV D    E DTPTTRPVS  APRN PSLGQTS S GR+LAPVAEG PVSLAAAAAAPAALETAE LLSMSPSVRHG G LE                      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  AC DPVGTPNSTAS SRRLGPGSPSSAT L    XXXXXXXXX              XXXXXXXXXXXXXXXXXXXXXXXXXXXX   RRPATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                  SS  RVRRTSRKGWCSPDLAAGQA AACLSY+LGETQHPELDLDPDLGELLDP+EGSSGAGSAG       XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX             R+  Q SD VEGGPSQGQGRXXXXXXXX  S   E++EGM VFDISALSAGSNSGD+GM
Sbjct:    1 MDDVGVDDHR--EGDTPTTRPVSHQAPRNTPSLGQTSSSRGRELAPVAEGPPVSLAAAAAAPAALETAETLLSMSPSVRHGSGSLEHGRDGGAPPARPTSSVSRRDGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXACDDPVGTPNSTASFSRRLGPGSPSSATGLLRTTXXXXXXXXXXXXXXXXXX----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRRPATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLGSSSRSSTPASKEAPSSS--RVRRTSRKGWCSPDLAAGQAYAACLSYMLGETQHPELDLDPDLGELLDPNEGSSGAGSAGDGGGKVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRDERQVSDDVEGGPSQGQGRXXXXXXXXISSSANEASEGMEVFDISALSAGSNSGDNGM 553
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BLAST of mRNA_E-fasciculatus_F_contig16072.4155.1 vs. uniprot
Match: A0A6H5KV73_9PHAE (BRCT domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KV73_9PHAE)

HSP 1 Score: 355 bits (911), Expect = 6.830e-105
Identity = 420/563 (74.60%), Postives = 428/563 (76.02%), Query Frame = 1 
Query:    1 MDDIGVVDDGGGEADTPTTRPVSRLAPRNGPSLGQTSGSGGRQLAPVAEGRPVSLAAAAA-APAALETAEALLSMSPSVRHGGGFLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXACGDPVGTPNSTASSSRRLGPGSPSSATDLXXXXXXXXXXXXXSYRSPALARPPNASXXXXXXXXXXXXXXXXXXXXXXXXXXXXHTA--------RRPATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSLSRVRRTSRKGWCSPDLAAGQANAACLSYLLGETQHPELDLDPDLGELLDPDEGSSGAGSAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-TNRGDKGRNRPQASDGVEGGPSQGQGRXXXXXXXXXXSESTEGMN---VFDISALSAGSNSGDSGM 1650
            MDD+GV D    E DTPTT PVS  APRN PSLGQTS SGG + APV +G PVSLAAAAA APAALETAEALLSMSPSVRHGGG LEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  AC DPVGTPNSTAS SRRLGPGSPSSAT L     XXXXXXXX              XXXXXXXXXXXXXXXXXXXXXXXXXXXX           R PATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRP+GTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX          SS SRVRRTSRKGWCSPDLAAGQA AACLSY+LGETQHPELDLDPDLGELLDP+EGSS AGSAG  XXXXXXXXXXXXXXXXXXXXXXXXXXX                                                  T RGD+GRN  QASDGVEGGPSQGQGR  XXXXXXXX           VFDISA SAGSNSGD+GM
Sbjct:  640 MDDVGVDDHR--EGDTPTTTPVSHQAPRNPPSLGQTSSSGGHEFAPVRKGPPVSLAAAAATAPAALETAEALLSMSPSVRHGGGSLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRGACDDPVGTPNSTASLSRRLGPGSPSSATGLLRTTRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRPPATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPNGTCDVKYIVHHGREKGVPAGIISFYAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSTPASKGAPSSSSRVRRTSRKGWCSPDLAAGQAYAACLSYMLGETQHPELDLDPDLGELLDPNEGSSRAGSAGDGXXXXXXXXXXXXXXXXXXXXXXXXXXXGAKEGDDGVTVVVSDRGVGSSGGEGGQGSVKGVVGKRKRIARGGGVDDDDTTRGDRGRNERQASDGVEGGPSQGQGRRHXXXXXXXXXXXXXXXXXXXVFDISASSAGSNSGDNGM 1200
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BLAST of mRNA_E-fasciculatus_F_contig16072.4155.1 vs. uniprot
Match: A0A485KVR3_9STRA (Aste57867_12351 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KVR3_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 7.890e-6
Identity = 30/60 (50.00%), Postives = 37/60 (61.67%), Query Frame = 1 
Query:  706 FKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVD 885
             K G LV V  R  PG+NKEGGVA+V  V  DGT +VKY+V  GR++ +P   I   A D
Sbjct:   32 LKQGTLVMVDPRFWPGMNKEGGVARVERVNQDGTVNVKYVVVGGRDRNIPPACIHVTAAD 91
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BLAST of mRNA_E-fasciculatus_F_contig16072.4155.1 vs. uniprot
Match: A0A1Z5KMS0_FISSO (BRCA1-associated RING domain protein 1 n=1 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KMS0_FISSO)

HSP 1 Score: 57.8 bits (138), Expect = 4.860e-5
Identity = 29/61 (47.54%), Postives = 41/61 (67.21%), Query Frame = 1 
Query:  694 ATPSFKAGDLVSVPSRSSPGVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFY 876
            AT SFK GD+++V  R+ PG+NK+GG+AKV  V  D + DV Y++  G+EK V A   S +
Sbjct:  407 ATASFKVGDIIAVKPRTWPGINKQGGIAKVIKVNEDNSYDVSYVLG-GKEKRVDAVFCSIH 466
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig16072.4155.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D8LPM9_ECTSI1.300e-10878.25BRCT domain-containing protein n=1 Tax=Ectocarpus ... [more]
A0A6H5KV73_9PHAE6.830e-10574.60BRCT domain-containing protein n=1 Tax=Ectocarpus ... [more]
A0A485KVR3_9STRA7.890e-650.00Aste57867_12351 protein n=1 Tax=Aphanomyces stella... [more]
A0A1Z5KMS0_FISSO4.860e-547.54BRCA1-associated RING domain protein 1 n=1 Tax=Fis... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig16072contigE-fasciculatus_F_contig16072:1..1650 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score362.8
Seed ortholog evalue2.7e-97
Seed eggNOG ortholog2880.D8LPM9
KEGG koko:K10605,ko:K19403
KEGG Pathwayko01524,ko03440,ko03460,ko04120,ko04151,ko05206,ko05224,map01524,map03440,map03460,map04120,map04151,map05206,map05224
KEGG ModuleM00295
EggNOG free text desc.E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator
EggNOG OGsKOG4362@1,KOG4362@2759
EC2.3.2.27
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01009,ko03036,ko03400,ko04121
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionBRCT domain-containing protein
Ec32 orthologEc-06_004760.1
Exons1
Model size1650
Cds size1650
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928074.6725125-CDS-E-fasciculatus_F_contig16072:0..16501622928074.6725125-CDS-E-fasciculatus_F_contig16072:0..1650Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16072 1..1650 -
1695995812.7437696-CDS-E-fasciculatus_F_contig16072:0..16501695995812.7437696-CDS-E-fasciculatus_F_contig16072:0..1650Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16072 1..1650 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig16072.4155.1prot_E-fasciculatus_F_contig16072.4155.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig16072 1..1650 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig16072.4155.1

>prot_E-fasciculatus_F_contig16072.4155.1 ID=prot_E-fasciculatus_F_contig16072.4155.1|Name=mRNA_E-fasciculatus_F_contig16072.4155.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=550bp
MDDIGVVDDGGGEADTPTTRPVSRLAPRNGPSLGQTSGSGGRQLAPVAEG
RPVSLAAAAAAPAALETAEALLSMSPSVRHGGGFLEHHRDGGAPPARPTT
SVSRRDGDGDDMAAAAGGCGGGSKPSVPPTEAEAKKEEGGACGDPVGTPN
STASSSRRLGPGSPSSATDLLRTTRRAMPPPPPSYRSPALARPPNASPSS
STSTPPPPPAPLPSRTPPPPPPPPPHTARRPATPSFKAGDLVSVPSRSSP
GVNKEGGVAKVTLVRPDGTCDVKYIVHHGREKGVPAGIISFYAVDDDDEE
SSSAGSGGDGRRERGGGGGRGAVAAGSSGSLSRSSSPASKEVPPPSSLSR
VRRTSRKGWCSPDLAAGQANAACLSYLLGETQHPELDLDPDLGELLDPDE
GSSGAGSAGGGKVDESRREDGASLAAAPAATAAEEEAKEGDDEVTVVASS
RGVGSSGGEGRQGGSKGVVGKRKRMTRGGGGGGDDDDTNRGDKGRNRPQA
SDGVEGGPSQGQGRRRRRRRSSSASESTEGMNVFDISALSAGSNSGDSGM
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mRNA from alignment at E-fasciculatus_F_contig16072:1..1650-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig16072.4155.1 ID=mRNA_E-fasciculatus_F_contig16072.4155.1|Name=mRNA_E-fasciculatus_F_contig16072.4155.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1650bp|location=Sequence derived from alignment at E-fasciculatus_F_contig16072:1..1650- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGGACGATATCGGGGTAGTCGACGACGGCGGCGGCGAAGCGGACACGCC
GACAACAAGGCCGGTGTCGCGCCTGGCGCCGAGAAACGGCCCCTCGCTAG
GTCAAACCAGCGGCAGCGGCGGCCGTCAGTTGGCGCCGGTTGCGGAAGGG
CGGCCGGTTTCGCTTGCTGCCGCCGCCGCTGCCCCCGCTGCGCTCGAGAC
CGCAGAGGCCTTGCTCTCGATGTCCCCTTCCGTGCGCCACGGTGGTGGTT
TTCTCGAGCATCACCGCGATGGCGGCGCGCCTCCCGCTCGACCGACGACT
AGCGTCAGCAGGCGGGACGGCGACGGAGATGACATGGCCGCTGCCGCCGG
TGGTTGTGGTGGTGGCTCGAAACCGTCGGTACCTCCCACAGAAGCAGAAG
CAAAGAAAGAAGAGGGTGGTGCTTGTGGTGACCCCGTGGGCACTCCGAAC
AGCACGGCATCCTCGTCGCGACGGCTCGGACCCGGAAGCCCCTCATCGGC
GACAGACTTGCTACGGACAACGCGGCGGGCAATGCCACCGCCGCCGCCGT
CCTACCGCTCCCCGGCCTTGGCGCGCCCTCCGAACGCGTCCCCGTCGTCG
TCGACGTCGACGCCTCCTCCTCCTCCTGCCCCGTTGCCGTCGCGAACGCC
TCCACCACCGCCGCCGCCGCCGCCGCATACGGCACGGCGGCCCGCAACCC
CGTCGTTCAAGGCGGGCGATTTGGTCAGCGTGCCCTCCCGCTCCTCCCCC
GGGGTAAACAAGGAAGGCGGCGTGGCGAAGGTGACGCTCGTCCGCCCGGA
CGGGACGTGCGACGTCAAGTACATCGTGCACCACGGCAGGGAGAAAGGCG
TTCCGGCCGGCATTATTTCCTTCTATGCGGTGGACGACGACGACGAAGAG
AGCAGCAGCGCCGGTAGCGGCGGCGACGGTAGGCGCGAACGCGGAGGAGG
GGGGGGGCGGGGCGCCGTTGCCGCCGGTTCCTCAGGGTCTTTGTCACGGT
CTTCTTCGCCGGCGTCGAAGGAGGTGCCGCCGCCGTCGTCATTGTCGCGC
GTGCGCAGGACGTCTCGCAAGGGGTGGTGCTCCCCGGACCTGGCGGCCGG
CCAGGCCAACGCGGCCTGCCTCAGCTACCTGCTTGGGGAGACCCAGCACC
CGGAGCTAGACCTGGACCCTGACCTCGGCGAGCTGCTTGACCCGGACGAG
GGCAGCAGCGGCGCCGGCAGCGCCGGTGGCGGCAAGGTAGACGAGAGTCG
AAGGGAGGACGGAGCTTCGCTGGCGGCAGCGCCGGCGGCAACGGCGGCGG
AAGAAGAGGCAAAGGAAGGCGACGACGAAGTCACCGTCGTGGCATCAAGC
CGGGGAGTTGGAAGCAGCGGCGGCGAGGGGCGGCAAGGAGGCAGTAAGGG
CGTCGTCGGCAAGAGGAAGCGCATGACACGTGGTGGTGGTGGTGGTGGTG
ATGACGACGACACCAACAGGGGTGATAAAGGCAGAAATCGACCGCAAGCC
TCTGACGGCGTTGAGGGAGGACCTTCGCAAGGGCAAGGACGCCGCCGCCG
TCGCCGCCGCAGCAGCAGCGCCAGCGAAAGCACCGAAGGCATGAACGTTT
TCGATATTTCGGCCTTGAGCGCGGGGTCGAACAGCGGGGATAGTGGCATG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig16072:1..1650-

>mRNA_E-fasciculatus_F_contig16072.4155.1 ID=mRNA_E-fasciculatus_F_contig16072.4155.1|Name=mRNA_E-fasciculatus_F_contig16072.4155.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=3300bp|location=Sequence derived from alignment at E-fasciculatus_F_contig16072:1..1650- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGGACGATATCGGGGTAGTCGACGACGGCGGCGGCGAAGCGGACACGCC
GACAACAAGGCCGGTGTCGCGCCTGGCGCCGAGAAACGGCCCCTCGCTAG
GTCAAACCAGCGGCAGCGGCGGCCGTCAGTTGGCGCCGGTTGCGGAAGGG
CGGCCGGTTTCGCTTGCTGCCGCCGCCGCTGCCCCCGCTGCGCTCGAGAC
CGCAGAGGCCTTGCTCTCGATGTCCCCTTCCGTGCGCCACGGTGGTGGTT
TTCTCGAGCATCACCGCGATGGCGGCGCGCCTCCCGCTCGACCGACGACT
AGCGTCAGCAGGCGGGACGGCGACGGAGATGACATGGCCGCTGCCGCCGG
TGGTTGTGGTGGTGGCTCGAAACCGTCGGTACCTCCCACAGAAGCAGAAG
CAAAGAAAGAAGAGGGTGGTGCTTGTGGTGACCCCGTGGGCACTCCGAAC
AGCACGGCATCCTCGTCGCGACGGCTCGGACCCGGAAGCCCCTCATCGGC
GACAGACTTGCTACGGACAACGCGGCGGGCAATGCCACCGCCGCCGCCGT
CCTACCGCTCCCCGGCCTTGGCGCGCCCTCCGAACGCGTCCCCGTCGTCG
TCGACGTCGACGCCTCCTCCTCCTCCTGCCCCGTTGCCGTCGCGAACGCC
TCCACCACCGCCGCCGCCGCCGCCGCATACGGCACGGCGGCCCGCAACCC
CGTCGTTCAAGGCGGGCGATTTGGTCAGCGTGCCCTCCCGCTCCTCCCCC
GGGGTAAACAAGGAAGGCGGCGTGGCGAAGGTGACGCTCGTCCGCCCGGA
CGGGACGTGCGACGTCAAGTACATCGTGCACCACGGCAGGGAGAAAGGCG
TTCCGGCCGGCATTATTTCCTTCTATGCGGTGGACGACGACGACGAAGAG
AGCAGCAGCGCCGGTAGCGGCGGCGACGGTAGGCGCGAACGCGGAGGAGG
GGGGGGGCGGGGCGCCGTTGCCGCCGGTTCCTCAGGGTCTTTGTCACGGT
CTTCTTCGCCGGCGTCGAAGGAGGTGCCGCCGCCGTCGTCATTGTCGCGC
GTGCGCAGGACGTCTCGCAAGGGGTGGTGCTCCCCGGACCTGGCGGCCGG
CCAGGCCAACGCGGCCTGCCTCAGCTACCTGCTTGGGGAGACCCAGCACC
CGGAGCTAGACCTGGACCCTGACCTCGGCGAGCTGCTTGACCCGGACGAG
GGCAGCAGCGGCGCCGGCAGCGCCGGTGGCGGCAAGGTAGACGAGAGTCG
AAGGGAGGACGGAGCTTCGCTGGCGGCAGCGCCGGCGGCAACGGCGGCGG
AAGAAGAGGCAAAGGAAGGCGACGACGAAGTCACCGTCGTGGCATCAAGC
CGGGGAGTTGGAAGCAGCGGCGGCGAGGGGCGGCAAGGAGGCAGTAAGGG
CGTCGTCGGCAAGAGGAAGCGCATGACACGTGGTGGTGGTGGTGGTGGTG
ATGACGACGACACCAACAGGGGTGATAAAGGCAGAAATCGACCGCAAGCC
TCTGACGGCGTTGAGGGAGGACCTTCGCAAGGGCAAGGACGCCGCCGCCG
TCGCCGCCGCAGCAGCAGCGCCAGCGAAAGCACCGAAGGCATGAACGTTT
TCGATATTTCGGCCTTGAGCGCGGGGTCGAACAGCGGGGATAGTGGCATG
ATGGACGATATCGGGGTAGTCGACGACGGCGGCGGCGAAGCGGACACGCC
GACAACAAGGCCGGTGTCGCGCCTGGCGCCGAGAAACGGCCCCTCGCTAG
GTCAAACCAGCGGCAGCGGCGGCCGTCAGTTGGCGCCGGTTGCGGAAGGG
CGGCCGGTTTCGCTTGCTGCCGCCGCCGCTGCCCCCGCTGCGCTCGAGAC
CGCAGAGGCCTTGCTCTCGATGTCCCCTTCCGTGCGCCACGGTGGTGGTT
TTCTCGAGCATCACCGCGATGGCGGCGCGCCTCCCGCTCGACCGACGACT
AGCGTCAGCAGGCGGGACGGCGACGGAGATGACATGGCCGCTGCCGCCGG
TGGTTGTGGTGGTGGCTCGAAACCGTCGGTACCTCCCACAGAAGCAGAAG
CAAAGAAAGAAGAGGGTGGTGCTTGTGGTGACCCCGTGGGCACTCCGAAC
AGCACGGCATCCTCGTCGCGACGGCTCGGACCCGGAAGCCCCTCATCGGC
GACAGACTTGCTACGGACAACGCGGCGGGCAATGCCACCGCCGCCGCCGT
CCTACCGCTCCCCGGCCTTGGCGCGCCCTCCGAACGCGTCCCCGTCGTCG
TCGACGTCGACGCCTCCTCCTCCTCCTGCCCCGTTGCCGTCGCGAACGCC
TCCACCACCGCCGCCGCCGCCGCCGCATACGGCACGGCGGCCCGCAACCC
CGTCGTTCAAGGCGGGCGATTTGGTCAGCGTGCCCTCCCGCTCCTCCCCC
GGGGTAAACAAGGAAGGCGGCGTGGCGAAGGTGACGCTCGTCCGCCCGGA
CGGGACGTGCGACGTCAAGTACATCGTGCACCACGGCAGGGAGAAAGGCG
TTCCGGCCGGCATTATTTCCTTCTATGCGGTGGACGACGACGACGAAGAG
AGCAGCAGCGCCGGTAGCGGCGGCGACGGTAGGCGCGAACGCGGAGGAGG
GGGGGGGCGGGGCGCCGTTGCCGCCGGTTCCTCAGGGTCTTTGTCACGGT
CTTCTTCGCCGGCGTCGAAGGAGGTGCCGCCGCCGTCGTCATTGTCGCGC
GTGCGCAGGACGTCTCGCAAGGGGTGGTGCTCCCCGGACCTGGCGGCCGG
CCAGGCCAACGCGGCCTGCCTCAGCTACCTGCTTGGGGAGACCCAGCACC
CGGAGCTAGACCTGGACCCTGACCTCGGCGAGCTGCTTGACCCGGACGAG
GGCAGCAGCGGCGCCGGCAGCGCCGGTGGCGGCAAGGTAGACGAGAGTCG
AAGGGAGGACGGAGCTTCGCTGGCGGCAGCGCCGGCGGCAACGGCGGCGG
AAGAAGAGGCAAAGGAAGGCGACGACGAAGTCACCGTCGTGGCATCAAGC
CGGGGAGTTGGAAGCAGCGGCGGCGAGGGGCGGCAAGGAGGCAGTAAGGG
CGTCGTCGGCAAGAGGAAGCGCATGACACGTGGTGGTGGTGGTGGTGGTG
ATGACGACGACACCAACAGGGGTGATAAAGGCAGAAATCGACCGCAAGCC
TCTGACGGCGTTGAGGGAGGACCTTCGCAAGGGCAAGGACGCCGCCGCCG
TCGCCGCCGCAGCAGCAGCGCCAGCGAAAGCACCGAAGGCATGAACGTTT
TCGATATTTCGGCCTTGAGCGCGGGGTCGAACAGCGGGGATAGTGGCATG
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