mRNA_E-fasciculatus_F_contig13856.2840.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig13856.2840.1
Unique NamemRNA_E-fasciculatus_F_contig13856.2840.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A6H5KP19_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KP19_9PHAE)

HSP 1 Score: 202 bits (513), Expect = 6.720e-61
Identity = 98/101 (97.03%), Postives = 101/101 (100.00%), Query Frame = 3 
Query:  372 MDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            MDRTRAAVQLIA+ARATAT+IHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPG+CIPLHDHPDM
Sbjct:    1 MDRTRAAVQLIAVARATATSIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGSCIPLHDHPDM 101
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: D8LBN7_ECTSI (Protein C10orf22, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBN7_ECTSI)

HSP 1 Score: 153 bits (386), Expect = 1.080e-44
Identity = 72/73 (98.63%), Postives = 73/73 (100.00%), Query Frame = 3 
Query:  456 MTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            MTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGAC+PLHDHPDM
Sbjct:    1 MTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACMPLHDHPDM 73
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: W7TJB2_9STRA (Uncharacterized protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TJB2_9STRA)

HSP 1 Score: 72.4 bits (176), Expect = 3.360e-11
Identity = 43/124 (34.68%), Postives = 59/124 (47.58%), Query Frame = 3 
Query:  303 ERCERLCPQKARDVGSAGGRRIPMDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            E   R+  ++  D G  GG R  + R RA                  P   M    + PI++ +  +    LGM+   HP++ PP+    EI YL + +E  F +GVFVLPP A IPLHDHP M
Sbjct:  116 EELNRISRKEEGDKGGWGGGRRRLGRRRA------------------PAFAMGMTQVAPIQKILDQIELHHLGMIAPVHPHLQPPTSRPSEIHYLCLLEEEDFHVGVFVLPPEARIPLHDHPGM 221
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A8K1FI05_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FI05_PYTOL)

HSP 1 Score: 68.6 bits (166), Expect = 3.830e-10
Identity = 37/81 (45.68%), Postives = 49/81 (60.49%), Query Frame = 3 
Query:  456 MTPEDIQPIEQAMAMLTPEALGM-VPYAHPYISPPSILS-------GEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            +TPE+I+PI+     L PE   + VP A       +  S         +RY HI ++ATFS+G+FVLPPGA IPLHDHP+M
Sbjct:   77 LTPEEIEPIKALCDQLLPEHFQLKVPEAQGKNGTSTATSPRSRPRRNRVRYQHIWEDATFSMGIFVLPPGASIPLHDHPEM 157
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A1W0A2F7_9STRA (Uncharacterized protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A2F7_9STRA)

HSP 1 Score: 67.4 bits (163), Expect = 2.180e-9
Identity = 38/90 (42.22%), Postives = 54/90 (60.00%), Query Frame = 3 
Query:  414 RATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGM-VPYAHPYISPPSILSGE--IRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            RA    + S  G  +T E+I+PI++    + P  +G+ VP A    +      G   IRY+HI ++A  SIG+FVLPPGA IPLH+HP+M
Sbjct:    9 RAVVHAVRSVRGRALTIEEIEPIKELCESILPVHVGLEVPTA---ATAKDAFKGSKAIRYIHIYEDAQVSIGIFVLPPGASIPLHNHPEM 95
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A835YIT4_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YIT4_9STRA)

HSP 1 Score: 67.0 bits (162), Expect = 2.650e-9
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = 3 
Query:  468 DIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSG-EIRYLHIAQ-EATFSIGVFVLPPGACIPLHDHPDM 674
            DI  + +A+  +T E LG+ P  H  + P ++ S  +IRYLH+ + E  +SIG+F+LPPGA +PLHDHP M
Sbjct:  312 DIAAVTRALEAITAEDLGLKPMGHMMVVPRTLHSSTDIRYLHVTEREDQYSIGIFLLPPGAEMPLHDHPYM 382
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A6P9D7R7_PANGU (2-aminoethanethiol dioxygenase n=1 Tax=Pantherophis guttatus TaxID=94885 RepID=A0A6P9D7R7_PANGU)

HSP 1 Score: 64.7 bits (156), Expect = 6.400e-9
Identity = 36/101 (35.64%), Postives = 50/101 (49.50%), Query Frame = 3 
Query:  372 MDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            M R   A  LI      A        GE    ++Q + + +  ++ E LG+ P     +  P+     + Y+HI + A FS+GVFVL  GACIPLHDHP M
Sbjct:    1 MPRDEMAASLIQRVARQARATFGSGSGEAAGSELQRLRELLGQVSAEDLGLQPRGPSPVPGPN--QPPVSYMHICETARFSMGVFVLRAGACIPLHDHPGM 99
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: UPI00192F8016 (2-aminoethanethiol dioxygenase n=1 Tax=Crotalus tigris TaxID=88082 RepID=UPI00192F8016)

HSP 1 Score: 64.3 bits (155), Expect = 8.690e-9
Identity = 37/103 (35.92%), Postives = 53/103 (51.46%), Query Frame = 3 
Query:  366 IPMDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            +P D   AA  +  +AR    T  +  G    P ++Q + + +  +  E LG+ P     +  P      + Y+HI + A FS+GVFVL  GACIPLHDHP M
Sbjct:    1 MPRDEMAAASLIQRVARQARATFGA--GAGEAPGELQRLRELLGQVRAEDLGLQPRGPSPVPGPR--QPPVSYMHICETARFSMGVFVLRAGACIPLHDHPGM 99
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: UPI0013778AA7 (2-aminoethanethiol dioxygenase n=1 Tax=Thamnophis elegans TaxID=35005 RepID=UPI0013778AA7)

HSP 1 Score: 63.9 bits (154), Expect = 1.190e-8
Identity = 40/102 (39.22%), Postives = 55/102 (53.92%), Query Frame = 3 
Query:  372 MDRTRAAVQLIA-IARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            M R   A  LI  +AR    T  +   GE + E +Q + + +  ++ E LG+ P   P + P       + Y+HI + A FS+GVFVL  GACIPLHDHP M
Sbjct:    1 MPRDEMAASLIQRVARQARATFGAGSSGEASGE-LQRLRELLGQVSAEDLGLQPRG-PSLGP-GASQPPVSYMHICETARFSMGVFVLRAGACIPLHDHPGM 99
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BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Match: A0A851PFY5_9GALL (AEDO dioxygenase (Fragment) n=1 Tax=Penelope pileata TaxID=1118817 RepID=A0A851PFY5_9GALL)

HSP 1 Score: 62.4 bits (150), Expect = 1.810e-8
Identity = 38/103 (36.89%), Postives = 53/103 (51.46%), Query Frame = 3 
Query:  366 IPMDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALGMVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPDM 674
            +P D   A +Q +  AR    T  S P G    ED+Q + Q +  +  E L + P      +P       + Y+HI +  +FS+GVF+L  GACIPLHDHP M
Sbjct:    1 MPRDNMAALIQRV--ARQARVTFRS-PAGPGFGEDLQRLRQLLDEVRAEDLHLAPRGPAAAAP-------VSYMHICETESFSMGVFLLRSGACIPLHDHPGM 93
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig13856.2840.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KP19_9PHAE6.720e-6197.03Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LBN7_ECTSI1.080e-4498.63Protein C10orf22, putative n=1 Tax=Ectocarpus sili... [more]
W7TJB2_9STRA3.360e-1134.68Uncharacterized protein n=2 Tax=Monodopsidaceae Ta... [more]
A0A8K1FI05_PYTOL3.830e-1045.68Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A1W0A2F7_9STRA2.180e-942.22Uncharacterized protein n=1 Tax=Thraustotheca clav... [more]
A0A835YIT4_9STRA2.650e-946.48Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A6P9D7R7_PANGU6.400e-935.642-aminoethanethiol dioxygenase n=1 Tax=Pantherophi... [more]
UPI00192F80168.690e-935.922-aminoethanethiol dioxygenase n=1 Tax=Crotalus ti... [more]
UPI0013778AA71.190e-839.222-aminoethanethiol dioxygenase n=1 Tax=Thamnophis ... [more]
A0A851PFY5_9GALL1.810e-836.89AEDO dioxygenase (Fragment) n=1 Tax=Penelope pilea... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig13856contigE-fasciculatus_F_contig13856:383..2019 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score65.1
Seed ortholog evalue2.2e-08
Seed eggNOG ortholog90675.XP_010492336.1
KEGG rclassRC00404
KEGG koko:K10712
KEGG ReactionR02467
KEGG Pathwayko00430,ko01100,map00430,map01100
GOsGO:0001666,GO:0003032,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0006091,GO:0006464,GO:0006807,GO:0006950,GO:0008150,GO:0008152,GO:0009061,GO:0009593,GO:0009628,GO:0009987,GO:0015980,GO:0018158,GO:0018171,GO:0018193,GO:0018198,GO:0019538,GO:0036211,GO:0036293,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0045333,GO:0050896,GO:0051606,GO:0055114,GO:0070482,GO:0070483,GO:0071704,GO:1901564
EggNOG free text desc.2-aminoethanethiol
EggNOG OGs37QHB@33090,3GGDR@35493,KOG4281@1,KOG4281@2759
EC1.13.11.19
COG Functional cat.S
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categorysignal peptide
Ec32 ortholog descriptionRmlC-like jelly roll fold
Ec32 orthologEc-27_003300.2
Exons3
Model size677
Cds size306
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig13856.2840.1prot_E-fasciculatus_F_contig13856.2840.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig13856 1312..2019 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927879.2897072-UTR-E-fasciculatus_F_contig13856:382..6511622927879.2897072-UTR-E-fasciculatus_F_contig13856:382..651Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13856 383..651 +
1695995734.8837364-UTR-E-fasciculatus_F_contig13856:382..6511695995734.8837364-UTR-E-fasciculatus_F_contig13856:382..651Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13856 383..651 +
1622927879.3081012-UTR-E-fasciculatus_F_contig13856:1209..13111622927879.3081012-UTR-E-fasciculatus_F_contig13856:1209..1311Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13856 1210..1311 +
1695995734.8999827-UTR-E-fasciculatus_F_contig13856:1209..13111695995734.8999827-UTR-E-fasciculatus_F_contig13856:1209..1311Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13856 1210..1311 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927879.322749-CDS-E-fasciculatus_F_contig13856:1311..14281622927879.322749-CDS-E-fasciculatus_F_contig13856:1311..1428Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13856 1312..1428 +
1695995734.9102168-CDS-E-fasciculatus_F_contig13856:1311..14281695995734.9102168-CDS-E-fasciculatus_F_contig13856:1311..1428Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13856 1312..1428 +
1622927879.3422885-CDS-E-fasciculatus_F_contig13856:1830..20191622927879.3422885-CDS-E-fasciculatus_F_contig13856:1830..2019Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13856 1831..2019 +
1695995734.9220784-CDS-E-fasciculatus_F_contig13856:1830..20191695995734.9220784-CDS-E-fasciculatus_F_contig13856:1830..2019Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13856 1831..2019 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig13856.2840.1

>prot_E-fasciculatus_F_contig13856.2840.1 ID=prot_E-fasciculatus_F_contig13856.2840.1|Name=mRNA_E-fasciculatus_F_contig13856.2840.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=102bp
MDRTRAAVQLIAIARATATTIHSRPGGEMTPEDIQPIEQAMAMLTPEALG
MVPYAHPYISPPSILSGEIRYLHIAQEATFSIGVFVLPPGACIPLHDHPD
M*
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mRNA from alignment at E-fasciculatus_F_contig13856:383..2019+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig13856.2840.1 ID=mRNA_E-fasciculatus_F_contig13856.2840.1|Name=mRNA_E-fasciculatus_F_contig13856.2840.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1637bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13856:383..2019+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GGTCAAACAGAAGGTCAAGGTTCCTCTAAGGCCCCCTACCCACCCCTCTC
CTGCCGTACTTGCCTTCAAATTGATAACATTCAACAAACACGACCATCAG
TTGGTTGTTTTGAAAAAAAGAAGGACCGTCCTCCTTCGATCCGCCGCCTC
GCTCCTTCTTTCACGCCACAGTCGGAGCTTCGAGGAATGATGCAAAATGG
CTGAAACAAAGTACTTGTGGGTGGGATCTACGGAACACGTGCCGGCAGGG
GACGACAGCACCTGCCTAGGTGTGTAAACATGTCGCGATAAACATCTCCC
ACACAGCAGCGGCCGGCGTAAAACAGCAGCACTTGTACAGCAGTATACGC
CGCGACCCAAACAGAACAATACATATTCACCGACGCGTGTATACCACACT
GCTTTTCTTCAACCCACGAGGTTGAGGTTTCCCTTCACACCTGGTCCTTG
TTCTCCTCCGTTCCGCACTCCGCACGCGGCACGTGCAATCATACATCCCC
CGTTACCGCACGAACGCACAGCCGACACACGGACAACTCAGGATCAGACG
TCTGGGTAACGACTACTGTGAGTGTGTGCGGCTTTTACCGCTCTCACGGC
TGCCGCCGCCGAGCAAAGCAAGCAAGGCGGCGGGGGCGACGCGGCGAGGG
GGGAGACGATCCAGAATTTTTCCAAGAAAATGATGCAACGTCACGAAAGG
AGAGCGCAACGCAACAGCAACGACGTGGTGTGGTGTGACATGTGAATTCG
ACACGCAAAAACATTCGTGTTCTCCCCCTCCGCCCTCTGCGCGATCCTCC
CCTCCGCTTGCACCGACCGCTCGGAAGGTGGATTCGTGTCGTCGTGCTGG
GTCAACGATCGAGCGTTGCGAAAGACTTTGCCCCCAAAAGGCTCGCGACG
TGGGAAGCGCTGGGGGGCGCAGGATACCGATGGACAGAACTCGAGCTGCA
GTTCAGCTGATTGCTATCGCCAGGGCAACCGCCACTACGATCCACTCAAG
GCCAGGAGGGGAGATGACGCCTGAGGATATCCAGCCCATCGAACAGGTGA
GAGGCATGGATGAGTTTTCCCGAGAGCTCTGGATTGTGGGCAGTGACTGG
GTATCCAGTCGGGCCGGGGGGGGGCTAGTGGGCGATGGTGTAGTCAACGT
GGCCTCACTATCAACGGTTGGATCCCGAATCTCGCAGCTGTTCGTGAGTT
CGGTAAGCCCCGTAAAGCCTTGCGCAAAACTATTCACCCCCCCTTTTGTG
CCGGGCCGCACGGCAGCACGGCAGCGTGTCGTTGCTACCCTCACAATCTG
GTGTAGTTCTGAATTCGTCCTGTGGACGCAGCGAGCTGCGGTCGGCACAG
GATGTATCTTGATCTACACCAGCAGTAGTCTACATCTCTCTCTGTTTCCG
CGCGACCATGCCACACCCGAGCCCGCTCCCCTGTTTGTTCCGGGCCAGGC
AATGGCGATGCTCACACCCGAGGCGCTGGGCATGGTGCCGTACGCCCACC
CATATATCAGCCCTCCATCGATCCTCTCGGGGGAGATTCGATATCTACAC
ATCGCGCAAGAGGCGACGTTCTCCATCGGAGTTTTCGTTCTGCCACCGGG
GGCTTGCATCCCGCTTCATGACCACCCAGACATGTGA
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig13856:383..2019+

>mRNA_E-fasciculatus_F_contig13856.2840.1 ID=mRNA_E-fasciculatus_F_contig13856.2840.1|Name=mRNA_E-fasciculatus_F_contig13856.2840.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=612bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13856:383..2019+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGGACAGAACTCGAGCTGCAGTTCAGCTGATTGCTATCGCCAGGGCAAC
CGCCACTACGATCCACTCAAGGCCAGGAGGGGAGATGACGCCTGAGGATA
TCCAGCCCATCGAACAGATGGACAGAACTCGAGCTGCAGTTCAGCTGATT
GCTATCGCCAGGGCAACCGCCACTACGATCCACTCAAGGCCAGGAGGGGA
GATGACGCCTGAGGATATCCAGCCCATCGAACAGGCAATGGCGATGCTCA
CACCCGAGGCGCTGGGCATGGTGCCGTACGCCCACCCATATATCAGCCCT
CCATCGATCCTCTCGGGGGAGATTCGATATCTACACATCGCGCAAGAGGC
GACGTTCTCCATCGGAGTTTTCGTTCTGCCACCGGGGGCTTGCATCCCGC
TTCATGACCACCCAGACATGTGAGCAATGGCGATGCTCACACCCGAGGCG
CTGGGCATGGTGCCGTACGCCCACCCATATATCAGCCCTCCATCGATCCT
CTCGGGGGAGATTCGATATCTACACATCGCGCAAGAGGCGACGTTCTCCA
TCGGAGTTTTCGTTCTGCCACCGGGGGCTTGCATCCCGCTTCATGACCAC
CCAGACATGTGA
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