mRNA_E-fasciculatus_F_contig12923.2215.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12923.2215.1
Unique NamemRNA_E-fasciculatus_F_contig12923.2215.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: D8LJB4_ECTSI (Rab-GAP TBC domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJB4_ECTSI)

HSP 1 Score: 145 bits (366), Expect = 4.380e-39
Identity = 69/70 (98.57%), Postives = 69/70 (98.57%), Query Frame = 1
Query:    1 MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLLRL 210
            MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVF DEEEAFWVFVGMLQRFRLRELYCPGMPLLRL
Sbjct:  464 MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFQDEEEAFWVFVGMLQRFRLRELYCPGMPLLRL 533          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A6H5JIN0_9PHAE (Rab-GAP TBC domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JIN0_9PHAE)

HSP 1 Score: 145 bits (366), Expect = 6.640e-39
Identity = 69/70 (98.57%), Postives = 69/70 (98.57%), Query Frame = 1
Query:    1 MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLLRL 210
            MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVF DEEEAFWVFVGMLQRFRLRELYCPGMPLLRL
Sbjct:  694 MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFQDEEEAFWVFVGMLQRFRLRELYCPGMPLLRL 763          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A7S2PZU8_9DINO (Hypothetical protein n=1 Tax=Brandtodinium nutricula TaxID=1333877 RepID=A0A7S2PZU8_9DINO)

HSP 1 Score: 63.9 bits (154), Expect = 1.440e-10
Identity = 30/62 (48.39%), Postives = 43/62 (69.35%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLL 204
            L NVLR  S F P+VGY QGM F+C  VL ++  EE+AF + + +L  +R+ +L+ PG+PLL
Sbjct:   48 LLNVLRAYSVFNPEVGYCQGMGFIC-GVLLMYMCEEDAFLMLISLLDNYRMSKLFMPGLPLL 108          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A078A7X3_STYLE (Tbc domain-containing protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078A7X3_STYLE)

HSP 1 Score: 63.5 bits (153), Expect = 4.270e-10
Identity = 28/61 (45.90%), Postives = 42/61 (68.85%), Query Frame = 1
Query:   28 VLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLLRL 210
            VLR  +  +P VGYVQG NF+  S++++  +EE+ FW+F+ +L    L +LY  GMPLL+L
Sbjct:  880 VLRAVAYHMPQVGYVQGFNFIAGSLMKLLKNEEQVFWMFINILTWRGLYQLYTDGMPLLKL 940          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A7R9Z0I5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9Z0I5_9STRA)

HSP 1 Score: 59.7 bits (143), Expect = 4.110e-9
Identity = 33/64 (51.56%), Postives = 40/64 (62.50%), Query Frame = 1
Query:   10 VSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQR--FRLRELYCPGM 195
            ++ LR VLR  ST+ PDVGY QGMNFV    L  F  EEEAFW+ V ++     R+R LY  GM
Sbjct:   51 IAALRRVLRAYSTYDPDVGYCQGMNFVAAMFL-TFMSEEEAFWLLVHVMNEEPCRMRGLYGEGM 113          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A813HJZ4_POLGL (Hypothetical protein n=1 Tax=Polarella glacialis TaxID=89957 RepID=A0A813HJZ4_POLGL)

HSP 1 Score: 60.1 bits (144), Expect = 6.160e-9
Identity = 28/62 (45.16%), Postives = 42/62 (67.74%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLL 204
            L NVLR  S F P+VGY QGM F+C  VL ++  E++AF + + +L+ +R+  L+ P +PLL
Sbjct:  133 LLNVLRAYSVFNPEVGYCQGMGFIC-GVLLMYMCEDDAFLMLISLLENYRMAGLFMPNLPLL 193          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A1E3P8T4_WICAA (Rab-GAP TBC domain-containing protein n=1 Tax=Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) TaxID=683960 RepID=A0A1E3P8T4_WICAA)

HSP 1 Score: 59.3 bits (142), Expect = 1.300e-8
Identity = 23/55 (41.82%), Postives = 38/55 (69.09%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELY 183
            L+N+L   S   P++GY QGMNF+  ++L V+P+EEEAFW F+G++     ++ +
Sbjct:  488 LKNILIAFSRKYPNIGYCQGMNFIAANLLLVYPNEEEAFWAFIGLVDNVLPKDFF 542          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A6P6RTB0_9EIME (ecotropic viral integration site 5 protein homolog n=1 Tax=Cyclospora cayetanensis TaxID=88456 RepID=A0A6P6RTB0_9EIME)

HSP 1 Score: 58.9 bits (141), Expect = 1.590e-8
Identity = 31/62 (50.00%), Postives = 41/62 (66.13%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLL 204
            L  VL+  + F PDVGY QGM FVC  VL +  +E EAF +   +L++F L+ LY PG+PLL
Sbjct:  111 LFKVLKAYAAFSPDVGYCQGMGFVC-GVLLLHMEEAEAFTLLACLLKKFCLKGLYQPGLPLL 171          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A7S1ALD3_NOCSC (Hypothetical protein n=1 Tax=Noctiluca scintillans TaxID=2966 RepID=A0A7S1ALD3_NOCSC)

HSP 1 Score: 58.9 bits (141), Expect = 1.660e-8
Identity = 28/62 (45.16%), Postives = 42/62 (67.74%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLL 204
            L +VLR  S F P+VGY QGM FVC  VL ++  E++AF + + +L+ +R+  L+ P +PLL
Sbjct:  157 LLSVLRAYSVFNPEVGYCQGMGFVC-GVLLMYMREDDAFLMLISLLENYRMAGLFMPNLPLL 217          
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Match: A0A8J4PMA1_9MYCE (Rab-GAP TBC domain-containing protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PMA1_9MYCE)

HSP 1 Score: 58.9 bits (141), Expect = 1.770e-8
Identity = 27/63 (42.86%), Postives = 39/63 (61.90%), Query Frame = 1
Query:   19 LRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVGMLQRFRLRELYCPGMPLLR 207
            L N+L+  S   P++GY QGM+F+C  +L    DE E FWVF  +++ F+L  LY   + LLR
Sbjct:  354 LFNILKAYSIMDPEIGYTQGMSFICAVILSEM-DELETFWVFTSVMKNFKLSSLYSNDLSLLR 415          
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12923.2215.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJB4_ECTSI4.380e-3998.57Rab-GAP TBC domain-containing protein n=1 Tax=Ecto... [more]
A0A6H5JIN0_9PHAE6.640e-3998.57Rab-GAP TBC domain-containing protein n=1 Tax=Ecto... [more]
A0A7S2PZU8_9DINO1.440e-1048.39Hypothetical protein n=1 Tax=Brandtodinium nutricu... [more]
A0A078A7X3_STYLE4.270e-1045.90Tbc domain-containing protein n=1 Tax=Stylonychia ... [more]
A0A7R9Z0I5_9STRA4.110e-951.56Hypothetical protein (Fragment) n=1 Tax=Pseudictyo... [more]
A0A813HJZ4_POLGL6.160e-945.16Hypothetical protein n=1 Tax=Polarella glacialis T... [more]
A0A1E3P8T4_WICAA1.300e-841.82Rab-GAP TBC domain-containing protein n=1 Tax=Wick... [more]
A0A6P6RTB0_9EIME1.590e-850.00ecotropic viral integration site 5 protein homolog... [more]
A0A7S1ALD3_NOCSC1.660e-845.16Hypothetical protein n=1 Tax=Noctiluca scintillans... [more]
A0A8J4PMA1_9MYCE1.770e-842.86Rab-GAP TBC domain-containing protein n=1 Tax=Poly... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12923contigE-fasciculatus_F_contig12923:112..663 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score147.1
Seed ortholog evalue3e-33
Seed eggNOG ortholog2880.D8LJB4
Preferred nameFASTKD3
KEGG koko:K19944
EggNOG free text desc.regulation of vesicle fusion
EggNOG OGsCOG5210@1,KOG1102@2759
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionRab-GTPase-TBC domain
Ec32 orthologEc-02_005450.1
Exons2
Model size210
Cds size210
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927796.5546684-CDS-E-fasciculatus_F_contig12923:111..2461622927796.5546684-CDS-E-fasciculatus_F_contig12923:111..246Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12923 112..246 -
1695995698.824964-CDS-E-fasciculatus_F_contig12923:111..2461695995698.824964-CDS-E-fasciculatus_F_contig12923:111..246Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12923 112..246 -
1622927796.6077604-CDS-E-fasciculatus_F_contig12923:588..6631622927796.6077604-CDS-E-fasciculatus_F_contig12923:588..663Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12923 589..663 -
1695995698.837454-CDS-E-fasciculatus_F_contig12923:588..6631695995698.837454-CDS-E-fasciculatus_F_contig12923:588..663Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12923 589..663 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12923.2215.1prot_E-fasciculatus_F_contig12923.2215.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12923 112..663 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12923.2215.1

>prot_E-fasciculatus_F_contig12923.2215.1 ID=prot_E-fasciculatus_F_contig12923.2215.1|Name=mRNA_E-fasciculatus_F_contig12923.2215.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=70bp
MTDVSPLRNVLRCTSTFVPDVGYVQGMNFVCRSVLQVFPDEEEAFWVFVG
MLQRFRLRELYCPGMPLLRL
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mRNA from alignment at E-fasciculatus_F_contig12923:112..663-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12923.2215.1 ID=mRNA_E-fasciculatus_F_contig12923.2215.1|Name=mRNA_E-fasciculatus_F_contig12923.2215.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=552bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12923:112..663- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGACGGATGTTTCTCCGCTTCGAAACGTGCTCCGGTGCACGTCGACCTT CGTGCCAGACGTGGGATACGTCCAGGTGAAAGCTGCCGACCCTTGGTCTC TTTGTCGTTGTTCGTTGTCACTGTCTTGCGGGCTCGTCGTCGTTCACATG TCACTGGTGTCGTGGTGTGGTACTTTTTGTTGTTCCTGTGTTGTGATTCT GATGGTGGTGATGGTGATGATGGTGATGCTGTTGCTGCGAGGCAGTGTCC GGCGGTATCTGTTGGTGTTTGGTGGTAATAGTACTAGTGTAACTCGACGC TTTTCACCAGGTAAAATATGGTCTGCCCGCTTTCAACATCGTGGGATTTC ACTTTGATCCCTTCCCCGCTCCCTGCCCCCCTTCCCCCCCCCCCTACGTG CACAATGCAACAAACAGGGCATGAACTTTGTATGTAGGTCCGTGCTACAG GTGTTCCCCGACGAAGAAGAGGCATTTTGGGTGTTCGTGGGCATGCTGCA GAGGTTCCGCCTGCGCGAGCTTTACTGTCCGGGGATGCCGTTGCTTCGGC TC
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12923:112..663-

>mRNA_E-fasciculatus_F_contig12923.2215.1 ID=mRNA_E-fasciculatus_F_contig12923.2215.1|Name=mRNA_E-fasciculatus_F_contig12923.2215.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=420bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12923:112..663- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGACGGATGTTTCTCCGCTTCGAAACGTGCTCCGGTGCACGTCGACCTT
CGTGCCAGACGTGGGATACGTCCAGATGACGGATGTTTCTCCGCTTCGAA
ACGTGCTCCGGTGCACGTCGACCTTCGTGCCAGACGTGGGATACGTCCAG
GGCATGAACTTTGTATGTAGGTCCGTGCTACAGGTGTTCCCCGACGAAGA
AGAGGCATTTTGGGTGTTCGTGGGCATGCTGCAGAGGTTCCGCCTGCGCG
AGCTTTACTGTCCGGGGATGCCGTTGCTTCGGCTCGGCATGAACTTTGTA
TGTAGGTCCGTGCTACAGGTGTTCCCCGACGAAGAAGAGGCATTTTGGGT
GTTCGTGGGCATGCTGCAGAGGTTCCGCCTGCGCGAGCTTTACTGTCCGG
GGATGCCGTTGCTTCGGCTC
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