mRNA_E-fasciculatus_F_contig12381.1835.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12381.1835.1
Unique NamemRNA_E-fasciculatus_F_contig12381.1835.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A6H5KYH2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYH2_9PHAE)

HSP 1 Score: 254 bits (648), Expect = 7.780e-81
Identity = 123/125 (98.40%), Postives = 124/125 (99.20%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPTST 393
            KSLEALKRCDNDP+VITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPT T
Sbjct:  341 KSLEALKRCDNDPHVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPTGT 465
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: D7FHD2_ECTSI (PRP1_N domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHD2_ECTSI)

HSP 1 Score: 255 bits (652), Expect = 3.950e-78
Identity = 124/125 (99.20%), Postives = 125/125 (100.00%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPTST 393
            KSLEALKRCDNDP+VITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPTST
Sbjct:  702 KSLEALKRCDNDPHVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPGGPTST 826
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A835YM25_9STRA (PRP1_N domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YM25_9STRA)

HSP 1 Score: 172 bits (435), Expect = 3.190e-47
Identity = 81/115 (70.43%), Postives = 96/115 (83.48%), Query Frame = 1 
Query:   16 GKSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGA 360
            GKS+EALKRCDNDP+VITAVAR F  DRKY KARKWFNRA+ LD + GDAWAA++AFELQQGT  +Q DV+ RCV AEP+HGELW SVSK T N+RLD+ ++L+KVVA YF+  A
Sbjct:  701 GKSVEALKRCDNDPHVITAVARLFEKDRKYPKARKWFNRAVALDADNGDAWAAFFAFELQQGTAEQQADVIARCVKAEPSHGELWCSVSKKTGNQRLDRETVLRKVVAQYFAPDA 815
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A7S2USD3_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2USD3_9STRA)

HSP 1 Score: 156 bits (394), Expect = 2.890e-44
Identity = 74/113 (65.49%), Postives = 89/113 (78.76%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDG 357
            +S+EALKRCDNDPNVI AVA  F  +RK AK+RKWFNRA+ LDP++GDAWA +YAFELQ G + +Q+++L RCVAAEP HGELW   SK TENRR +  +ILKKVVAA    G
Sbjct:  213 RSVEALKRCDNDPNVILAVAVLFQNERKVAKSRKWFNRAVALDPDLGDAWALFYAFELQHGKKEDQEELLKRCVAAEPTHGELWCGTSKKTENRRCNTETILKKVVAALAGIG 325
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A7S3XY73_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XY73_HETAK)

HSP 1 Score: 161 bits (408), Expect = 9.510e-44
Identity = 76/118 (64.41%), Postives = 93/118 (78.81%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVI 372
            +S+EALKRCDNDP+V+ AVAR F +DRK+AKARKWF RA+ L P +GDAWA +YAFEL+QGT  EQ+ VLDRCVAAEPAHGELW +V+K T NRRL  A +L+KVVA   +  A   +
Sbjct:  606 RSVEALKRCDNDPHVVLAVARLFASDRKHAKARKWFERAVALRPGLGDAWARFYAFELRQGTPEEQRAVLDRCVAAEPAHGELWQAVAKRTANRRLGPAEVLRKVVAELAAGAAAPAV 723
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A438KFT4_VITVI (Protein STABILIZED 1 n=1 Tax=Vitis vinifera TaxID=29760 RepID=A0A438KFT4_VITVI)

HSP 1 Score: 145 bits (367), Expect = 3.390e-42
Identity = 69/115 (60.00%), Postives = 83/115 (72.17%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAV 363
            KSL+ALK+CD+DP+VI AVA+ FW DRK  KAR W NRA+TL P++GD WA YY FE+Q G+E  QKDVL RCVAAEP HGE W  +SK  EN  L   +ILKK V A   + +V
Sbjct:   39 KSLDALKKCDHDPHVIAAVAKLFWHDRKVDKARTWLNRAVTLAPDIGDFWALYYKFEVQHGSEENQKDVLRRCVAAEPKHGEKWKVISKAVENSHLPTEAILKKAVVALGKEESV 153
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A2R6QX31_ACTCC (Uncharacterized protein n=1 Tax=Actinidia chinensis var. chinensis TaxID=1590841 RepID=A0A2R6QX31_ACTCC)

HSP 1 Score: 147 bits (370), Expect = 1.400e-40
Identity = 68/108 (62.96%), Postives = 81/108 (75.00%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAA 342
            KS++ALK+CD+DP+VI AVA+ FW DRK  KAR W NRA+TL P++GD WA YY FELQ GTE  QKDVL RC+AAEP HGE W ++SK  EN      +ILKKVV A
Sbjct:  217 KSMDALKKCDHDPHVIAAVAKLFWHDRKVDKARNWLNRAVTLAPDIGDFWALYYKFELQHGTEDNQKDVLKRCIAAEPKHGEKWQAISKAVENSHQPTEAILKKVVVA 324
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: UPI00098E1367 (protein STABILIZED1 n=1 Tax=Asparagus officinalis TaxID=4686 RepID=UPI00098E1367)

HSP 1 Score: 151 bits (381), Expect = 8.210e-40
Identity = 73/120 (60.83%), Postives = 84/120 (70.00%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPG 378
            KS +ALKRCD+DP+VI  VA+ FW DRK  KAR WFNRA+TL P++GD WA YY FELQ G E  QKDVL RCVAAEP HGE WTS+SK  EN  L   +ILKKVV A   +    +  G
Sbjct:  821 KSADALKRCDHDPHVIATVAKLFWHDRKVDKARSWFNRAVTLSPDVGDFWALYYKFELQHGNEDTQKDVLKRCVAAEPKHGERWTSISKAVENSHLLVEAILKKVVVALGKEENPAIADG 940
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A5P1FRW4_ASPOF (Uncharacterized protein n=1 Tax=Asparagus officinalis TaxID=4686 RepID=A0A5P1FRW4_ASPOF)

HSP 1 Score: 151 bits (381), Expect = 8.350e-40
Identity = 73/120 (60.83%), Postives = 84/120 (70.00%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVVVIPG 378
            KS +ALKRCD+DP+VI  VA+ FW DRK  KAR WFNRA+TL P++GD WA YY FELQ G E  QKDVL RCVAAEP HGE WTS+SK  EN  L   +ILKKVV A   +    +  G
Sbjct:  893 KSADALKRCDHDPHVIATVAKLFWHDRKVDKARSWFNRAVTLSPDVGDFWALYYKFELQHGNEDTQKDVLKRCVAAEPKHGERWTSISKAVENSHLLVEAILKKVVVALGKEENPAIADG 1012
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BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Match: A0A0C9S9J4_9SPER (TSA: Wollemia nobilis Ref_Wollemi_Transcript_239_3443 transcribed RNA sequence n=1 Tax=Wollemia nobilis TaxID=56998 RepID=A0A0C9S9J4_9SPER)

HSP 1 Score: 150 bits (380), Expect = 1.140e-39
Identity = 73/116 (62.93%), Postives = 84/116 (72.41%), Query Frame = 1 
Query:   19 KSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDPNMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENRRLDKASILKKVVAAYFSDGAVV 366
            KS++ALKRCD+DP VI AVA+ FW DRK  KAR WFNRA+TL P+ GD WA YY FELQ GTE  Q+DVL RC+AAEP HGE WTS+SK  EN      +ILKKVV A   +  VV
Sbjct:  903 KSMDALKRCDHDPYVIAAVAKLFWHDRKVDKARNWFNRAVTLAPDNGDFWAQYYKFELQHGTEEIQQDVLKRCIAAEPRHGERWTSISKAVENSHQPIEAILKKVVVAIGKEEGVV 1018
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12381.1835.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KYH2_9PHAE7.780e-8198.40Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FHD2_ECTSI3.950e-7899.20PRP1_N domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A835YM25_9STRA3.190e-4770.43PRP1_N domain-containing protein n=1 Tax=Tribonema... [more]
A0A7S2USD3_9STRA2.890e-4465.49Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S3XY73_HETAK9.510e-4464.41Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A438KFT4_VITVI3.390e-4260.00Protein STABILIZED 1 n=1 Tax=Vitis vinifera TaxID=... [more]
A0A2R6QX31_ACTCC1.400e-4062.96Uncharacterized protein n=1 Tax=Actinidia chinensi... [more]
UPI00098E13678.210e-4060.83protein STABILIZED1 n=1 Tax=Asparagus officinalis ... [more]
A0A5P1FRW4_ASPOF8.350e-4060.83Uncharacterized protein n=1 Tax=Asparagus officina... [more]
A0A0C9S9J4_9SPER1.140e-3962.93TSA: Wollemia nobilis Ref_Wollemi_Transcript_239_3... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12381contigE-fasciculatus_F_contig12381:2942..3646 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score256.5
Seed ortholog evalue6.7e-66
Seed eggNOG ortholog2880.D7FHD2
KEGG koko:K12855
KEGG Pathwayko03040,map03040
KEGG ModuleM00354
GOsGO:0000244,GO:0000375,GO:0000377,GO:0000387,GO:0000398,GO:0003674,GO:0003676,GO:0003712,GO:0003713,GO:0003723,GO:0003824,GO:0004553,GO:0004565,GO:0005102,GO:0005488,GO:0005515,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005681,GO:0005682,GO:0005684,GO:0005737,GO:0005773,GO:0006139,GO:0006259,GO:0006304,GO:0006305,GO:0006306,GO:0006355,GO:0006357,GO:0006396,GO:0006397,GO:0006403,GO:0006725,GO:0006807,GO:0006950,GO:0007275,GO:0008134,GO:0008150,GO:0008152,GO:0008380,GO:0009266,GO:0009409,GO:0009628,GO:0009791,GO:0009845,GO:0009889,GO:0009891,GO:0009892,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0015030,GO:0015925,GO:0016043,GO:0016070,GO:0016071,GO:0016458,GO:0016604,GO:0016607,GO:0016787,GO:0016798,GO:0019219,GO:0019222,GO:0022607,GO:0022613,GO:0022618,GO:0030312,GO:0030532,GO:0031047,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032259,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0034622,GO:0034641,GO:0035257,GO:0035258,GO:0043021,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0044728,GO:0044877,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0046540,GO:0048518,GO:0048519,GO:0048522,GO:0048583,GO:0048584,GO:0048856,GO:0050681,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051179,GO:0051252,GO:0051254,GO:0051427,GO:0060147,GO:0060148,GO:0060255,GO:0060964,GO:0060966,GO:0060968,GO:0065003,GO:0065007,GO:0070013,GO:0070920,GO:0071005,GO:0071011,GO:0071013,GO:0071704,GO:0071826,GO:0071840,GO:0071944,GO:0080090,GO:0080188,GO:0090304,GO:0090351,GO:0097159,GO:0097525,GO:0097526,GO:0120114,GO:0140110,GO:1901360,GO:1901363,GO:1902494,GO:1902680,GO:1903506,GO:1903508,GO:1903798,GO:1903800,GO:1990904,GO:2000112,GO:2000628,GO:2000630,GO:2000634,GO:2000636,GO:2000637,GO:2001141
EggNOG free text desc.spliceosomal tri-snRNP complex assembly
EggNOG OGsKOG0495@1,KOG0495@2759
COG Functional cat.A
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03041
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionpre-mRNA splicing factor, putative
Ec32 orthologEc-00_001250.1
Exons2
Model size399
Cds size399
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927745.0186977-CDS-E-fasciculatus_F_contig12381:2941..31741622927745.0186977-CDS-E-fasciculatus_F_contig12381:2941..3174Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12381 2942..3174 +
1695995676.3595378-CDS-E-fasciculatus_F_contig12381:2941..31741695995676.3595378-CDS-E-fasciculatus_F_contig12381:2941..3174Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12381 2942..3174 +
1622927745.0313005-CDS-E-fasciculatus_F_contig12381:3480..36461622927745.0313005-CDS-E-fasciculatus_F_contig12381:3480..3646Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12381 3481..3646 +
1695995676.3697808-CDS-E-fasciculatus_F_contig12381:3480..36461695995676.3697808-CDS-E-fasciculatus_F_contig12381:3480..3646Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12381 3481..3646 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12381.1835.1prot_E-fasciculatus_F_contig12381.1835.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12381 2942..3646 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12381.1835.1

>prot_E-fasciculatus_F_contig12381.1835.1 ID=prot_E-fasciculatus_F_contig12381.1835.1|Name=mRNA_E-fasciculatus_F_contig12381.1835.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=133bp
PTPPTGKSLEALKRCDNDPNVITAVARRFWADRKYAKARKWFNRAITLDP
NMGDAWAAYYAFELQQGTEVEQKDVLDRCVAAEPAHGELWTSVSKTTENR
RLDKASILKKVVAAYFSDGAVVVIPGGPTSTG*
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mRNA from alignment at E-fasciculatus_F_contig12381:2942..3646+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12381.1835.1 ID=mRNA_E-fasciculatus_F_contig12381.1835.1|Name=mRNA_E-fasciculatus_F_contig12381.1835.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=705bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12381:2942..3646+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CCAACACCCCCAACAGGCAAGTCCCTCGAGGCGCTCAAGCGGTGCGACAA
CGACCCGAACGTCATCACCGCCGTGGCGAGGCGTTTCTGGGCGGACCGCA
AGTACGCCAAGGCCCGCAAGTGGTTCAACCGCGCCATCACCCTGGACCCC
AACATGGGGGACGCGTGGGCGGCGTACTACGCGTTCGAGCTGCAGCAGGG
GACCGAGGTTGAGCAGAAGGACGTTCTGGACAGGTGTGTAACGTTACGTG
ATGCTTACTTGGCGGTACCCGTGGCCGTTGATTAGTGAGATACCACAAGT
GATCAATCATAGTGTGCAGTGTTTGTGTCTTTTGGTGCTGGTGTCATACT
GGTTTTTGCGTTGAAACATGTGTGTTGTTTTGTTTGGGTATTTTCTAGTT
TTCAGTTCGGGCGGCGCAATACATCGCGGTGACTCGGTGAGATGTGACTA
AATGCATAAGCGTTTTGGCGCCCAAAACACTATCGTGTGACGCGGTGAGA
TGCGACTAAAGTTCGTTTTTGCGCCCGAAAACGCGACAGGTGTGTTGCGG
CCGAGCCTGCCCACGGGGAGCTGTGGACGTCCGTATCCAAGACCACCGAG
AACAGACGGCTCGACAAGGCCAGCATCCTCAAGAAAGTCGTGGCCGCGTA
CTTTTCTGACGGCGCTGTGGTGGTCATTCCGGGAGGCCCGACAAGCACCG
GCTAG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12381:2942..3646+

>mRNA_E-fasciculatus_F_contig12381.1835.1 ID=mRNA_E-fasciculatus_F_contig12381.1835.1|Name=mRNA_E-fasciculatus_F_contig12381.1835.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=798bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12381:2942..3646+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CCAACACCCCCAACAGGCAAGTCCCTCGAGGCGCTCAAGCGGTGCGACAA
CGACCCGAACGTCATCACCGCCGTGGCGAGGCGTTTCTGGGCGGACCGCA
AGTACGCCAAGGCCCGCAAGTGGTTCAACCGCGCCATCACCCTGGACCCC
AACATGGGGGACGCGTGGGCGGCGTACTACGCGTTCGAGCTGCAGCAGGG
GACCGAGGTTGAGCAGAAGGACGTTCTGGACAGCCAACACCCCCAACAGG
CAAGTCCCTCGAGGCGCTCAAGCGGTGCGACAACGACCCGAACGTCATCA
CCGCCGTGGCGAGGCGTTTCTGGGCGGACCGCAAGTACGCCAAGGCCCGC
AAGTGGTTCAACCGCGCCATCACCCTGGACCCCAACATGGGGGACGCGTG
GGCGGCGTACTACGCGTTCGAGCTGCAGCAGGGGACCGAGGTTGAGCAGA
AGGACGTTCTGGACAGGTGTGTTGCGGCCGAGCCTGCCCACGGGGAGCTG
TGGACGTCCGTATCCAAGACCACCGAGAACAGACGGCTCGACAAGGCCAG
CATCCTCAAGAAAGTCGTGGCCGCGTACTTTTCTGACGGCGCTGTGGTGG
TCATTCCGGGAGGCCCGACAAGCACCGGCTAGGTGTGTTGCGGCCGAGCC
TGCCCACGGGGAGCTGTGGACGTCCGTATCCAAGACCACCGAGAACAGAC
GGCTCGACAAGGCCAGCATCCTCAAGAAAGTCGTGGCCGCGTACTTTTCT
GACGGCGCTGTGGTGGTCATTCCGGGAGGCCCGACAAGCACCGGCTAG
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