mRNA_E_fasciculatus_S2_contig8732.16839.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig8732.16839.1
Unique NamemRNA_E_fasciculatus_S2_contig8732.16839.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUD0_ECTSI)

HSP 1 Score: 194 bits (494), Expect = 5.290e-55
Identity = 100/134 (74.63%), Postives = 110/134 (82.09%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GG+ +Q   G FAVVLEYS  DNEL AQI+GDISTPAPWVALS+V SAV LMLLDFPGLRSRGSL CP+HGDAM LA+  TR GD +LE S CRQCS DT G+GAAAI LVRMVDIR DRDVI  EV+AR
Sbjct:  831 VLVHGGVGEQDRSGIFAVVLEYSPSDNELTAQIFGDISTPAPWVALSYVVSAVSLMLLDFPGLRSRGSLKCPQHGDAMPLANMVTRTGDKILEGSRCRQCSPDTRGLGAAAIDLVRMVDIRLDRDVIFREVKAR 964
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUC9_ECTSI)

HSP 1 Score: 190 bits (483), Expect = 1.560e-53
Identity = 99/134 (73.88%), Postives = 109/134 (81.34%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL D+     FAVVLEYS  DNEL AQI+GDISTPAPWVALS+V SAVRLMLLDFPGLRSRGSL CP+HGDAMLL +K T+AG  LLE SGC QCS DT G+GAAAI LV MVDIR DRDVI  +V+ R
Sbjct:  731 VLVHGGLGDEDGHEIFAVVLEYSSSDNELTAQIFGDISTPAPWVALSYVISAVRLMLLDFPGLRSRGSLKCPQHGDAMLLVNKMTQAGVKLLEPSGCPQCSPDTRGLGAAAIDLVSMVDIRLDRDVIFRDVKQR 864
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K516_9PHAE)

HSP 1 Score: 186 bits (471), Expect = 2.390e-52
Identity = 97/134 (72.39%), Postives = 106/134 (79.10%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+G    Q  R TFAVVLEYS  DNEL A+I+GDISTPA WVALS+V SAV+LML+DFPGLR +GSL CPEHGDAML  DKA R GD LLE S C QCS DTGG+GAAAI LVR VDIR DRDVI  EV+AR
Sbjct:  383 VLVHGSFGAQDGRRTFAVVLEYSSTDNELTAKIFGDISTPAAWVALSYVMSAVKLMLMDFPGLRWKGSLKCPEHGDAMLFVDKARRGGDKLLEGSRCPQCSVDTGGLGAAAIDLVRFVDIRLDRDVIFKEVKAR 516
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1K4_9PHAE)

HSP 1 Score: 183 bits (464), Expect = 4.790e-51
Identity = 96/134 (71.64%), Postives = 106/134 (79.10%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+G L  Q  R TFAVVLEYS  DNEL A+I+GDISTPA WVALS+V SAVRLML+DFPGLR +GSL CP+HGDAML  D A R GD LLE S C QCS DTGG+GAAAI LVR VDIR DRDVI  +V+AR
Sbjct:  522 VLVHGSLGVQDGRRTFAVVLEYSSTDNELTAKIFGDISTPAAWVALSYVMSAVRLMLVDFPGLRWKGSLKCPQHGDAMLFVDMARRGGDKLLEGSRCPQCSVDTGGLGAAAIDLVRFVDIRLDRDVIFNDVKAR 655
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJS7_ECTSI)

HSP 1 Score: 178 bits (451), Expect = 3.300e-49
Identity = 91/134 (67.91%), Postives = 104/134 (77.61%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL  Q ER TFAVV+EYS  DNEL+AQI+GD+STP PW A S+V SAV LML+DFPGL  +G L CP+HGD ML  +KATR GD  LE S C QCS DTGG+GAAAI L+R VDIR DRDVI  EV+AR
Sbjct:  835 VLVHGGLGVQDERRTFAVVVEYSSTDNELIAQIFGDVSTPGPWGATSYVMSAVSLMLVDFPGLGWKGYLECPQHGDGMLFVNKATRDGDKFLEGSRCPQCSADTGGLGAAAIDLLRFVDIRLDRDVIFNEVKAR 968
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUC3_ECTSI)

HSP 1 Score: 172 bits (437), Expect = 2.530e-47
Identity = 91/134 (67.91%), Postives = 101/134 (75.37%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            V V+GG   QG    FAVVLEYS  +NEL A+I+GDISTP PW+ALS+V SAV LM+ DFPGLRSRGSL CP+HGDAMLLA K  R GD  LE S C QCS DT G+GAAAI LVRMVDIR DRD I  EV+ R
Sbjct:  759 VFVHGGHGYQGGHEMFAVVLEYSSANNELTAKIFGDISTPTPWMALSYVMSAVSLMMRDFPGLRSRGSLKCPQHGDAMLLATKVPRPGDKFLEGSRCPQCSADTRGLGAAAIDLVRMVDIRLDRDEIFHEVKER 892
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LF46_ECTSI)

HSP 1 Score: 171 bits (433), Expect = 8.770e-47
Identity = 90/134 (67.16%), Postives = 104/134 (77.61%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL D   RG FAVV+EYS  DNEL AQI+GDISTPAPWVALS+V SAV LML+DFPGLR +G L CP+HG+ M  A+K  RAGD  LER  C +CS +T G+GAAAI LV +VDIR DRDVI  EV+AR
Sbjct:  901 VLVHGGLGDLDGRGIFAVVVEYSSTDNELTAQIFGDISTPAPWVALSYVMSAVSLMLVDFPGLRWKGCLKCPQHGNEMPFANKVNRAGDKFLERK-CPRCSPETRGLGAAAIDLVCLVDIRLDRDVIFREVKAR 1033
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0H4_9PHAE)

HSP 1 Score: 169 bits (427), Expect = 2.200e-46
Identity = 90/134 (67.16%), Postives = 101/134 (75.37%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL  Q  R TFAVVLEYS   NEL AQI+G ISTPA WVALS+V SAVRLML+DFPGLR +GSL CP+HGDAM   + A   GD LLE S C  CS DTGG+G AAI LVR+VDIR +RDVI   V+AR
Sbjct:  349 VLVHGGLGVQDGRSTFAVVLEYSSAGNELTAQIFGGISTPAAWVALSYVMSAVRLMLVDFPGLRWKGSLKCPQHGDAMPFVNMARSDGDKLLEGSRCPHCSEDTGGLGEAAIDLVRLVDIRLNRDVIFNGVKAR 482
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family, incomplete sequence n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G431_ECTSI)

HSP 1 Score: 166 bits (421), Expect = 3.570e-45
Identity = 87/134 (64.93%), Postives = 104/134 (77.61%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL D G RG FAVVLEYS  D++L  QI+GDI TPAPWVALS+V SAV LML+DFPGLR  G+L CP+HGDAML+A+K T+AG+  L+R  C +CS +T G+GAAAI L+RMVDIR DR  I  EV  R
Sbjct:  637 VLVHGGLGDLGGRGIFAVVLEYSSTDHKLNGQIFGDIGTPAPWVALSYVMSAVSLMLVDFPGLRWGGALKCPQHGDAMLMANKVTQAGETFLKRR-CPRCSTETKGLGAAAIDLLRMVDIRMDRGEIFDEVMER 769
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Match: LRR-GTPase (of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCS7_ECTSI)

HSP 1 Score: 166 bits (419), Expect = 6.800e-45
Identity = 87/134 (64.93%), Postives = 100/134 (74.63%), Query Frame = 1 
Query:    1 VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVASAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQCSHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR 402
            VLV+GGL D+   G FA VLEYS  +N L AQI+GD S  A WVALS+V SAV LMLLDFPGLR RGSL CP+HGD +LL +K TRAGD  L  S C+QCS DT  +GAAAI LVRMV++R DRDVI  EV+ R
Sbjct: 1067 VLVHGGLGDEDGYGIFAAVLEYSSAENGLTAQIFGDTSFSAAWVALSYVMSAVSLMLLDFPGLRWRGSLKCPQHGDTILLTNKVTRAGDKFLAGSRCQQCSADTRALGAAAIDLVRMVNVRLDRDVIFREVKER 1200
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig8732.16839.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
LRR-GTPase5.290e-5574.63of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]
LRR-GTPase1.560e-5373.88of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]
Uncharacterized2.390e-5272.39protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized4.790e-5171.64protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
LRR-GTPase3.300e-4967.91of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]
LRR-GTPase2.530e-4767.91of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]
LRR-GTPase8.770e-4767.16of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]
Uncharacterized2.200e-4667.16protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
LRR-GTPase3.570e-4564.93of the ROCO family, incomplete sequence n=1 Tax=Ec... [more]
LRR-GTPase6.800e-4564.93of the ROCO family n=1 Tax=Ectocarpus siliculosus ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig8732contigE_fasciculatus_S2_contig8732:4052..5563 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score194.9
Seed ortholog evalue2.4e-47
Seed eggNOG ortholog2880.D7FUD0
Model size404
KEGG rclassRC00049,RC00059,RC00171,RC00262,RC00397,RC00451,RC00714,RC00746,RC01248
KEGG koko:K01188
KEGG ReactionR00026,R02558,R02887,R02985,R03527,R04949,R04998,R10035,R10039,R10040
KEGG Pathwayko00460,ko00500,ko00940,ko01100,ko01110,map00460,map00500,map00940,map01100,map01110
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003002,GO:0003006,GO:0003674,GO:0004857,GO:0007275,GO:0007389,GO:0008150,GO:0009791,GO:0009908,GO:0022414,GO:0030234,GO:0032501,GO:0032502,GO:0043086,GO:0044092,GO:0048367,GO:0048608,GO:0048731,GO:0048832,GO:0048833,GO:0048856,GO:0050789,GO:0050790,GO:0050793,GO:0061458,GO:0065007,GO:0065009,GO:0090567,GO:0098772
Exons3
EggNOG OGs2CYK8@1,2S4YE@2759
EC3.2.1.21
Cds size402
CAZyGH1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842529.7631414-CDS-E_fasciculatus_S2_contig8732:4051..43001696842529.7631414-CDS-E_fasciculatus_S2_contig8732:4051..4300Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8732 4052..4300 +
1696842529.7783875-CDS-E_fasciculatus_S2_contig8732:5007..51311696842529.7783875-CDS-E_fasciculatus_S2_contig8732:5007..5131Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8732 5008..5131 +
1696842529.7932606-CDS-E_fasciculatus_S2_contig8732:5534..55631696842529.7932606-CDS-E_fasciculatus_S2_contig8732:5534..5563Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig8732 5535..5563 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig8732.16839.1prot_E_fasciculatus_S2_contig8732.16839.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig8732 4052..5563 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig8732.16839.1

>prot_E_fasciculatus_S2_contig8732.16839.1 ID=prot_E_fasciculatus_S2_contig8732.16839.1|Name=mRNA_E_fasciculatus_S2_contig8732.16839.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=134bp
VLVYGGLTDQGERGTFAVVLEYSLDDNELVAQIYGDISTPAPWVALSHVA
SAVRLMLLDFPGLRSRGSLNCPEHGDAMLLADKATRAGDNLLERSGCRQC
SHDTGGVGAAAIHLVRMVDIRQDRDVIAIEVRAR
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mRNA from alignment at E_fasciculatus_S2_contig8732:4052..5563+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig8732.16839.1 ID=mRNA_E_fasciculatus_S2_contig8732.16839.1|Name=mRNA_E_fasciculatus_S2_contig8732.16839.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=1512bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8732:4052..5563+ (Ectocarpus fasciculatus EfasUO2)
GTGCTTGTGTATGGAGGTCTCACCGACCAAGGCGAACGCGGGACATTTGC
TGTGGTTCTGGAATATTCGCTCGACGACAACGAACTTGTCGCTCAGATAT
ATGGCGATATCAGCACTCCAGCTCCGTGGGTGGCCCTTTCTCACGTAGCG
TCTGCCGTGAGGTTGATGCTGCTGGACTTTCCCGGTTTACGCTCGAGAGG
CTCTCTGAACTGTCCTGAGCATGGCGACGCAATGCTTTTGGCCGACAAGG
TGAGCCCCCGGAAATCTTGCGTAGTCTAGAAAAGCATCGAACGCCCCCGA
GGTGCGCACTTCTGGCCGCTTGAGCCGCAATCGGTTGCGTGCATTCTTTG
TACCGATGTCTGGCGGTTTCATGCCAACTTACTCTTGCGTCTTGGAGTCC
ATGTTTCAGAGTGGTGTTGCGGTTGGAGTTGGTAGATCTAACAGGGGGTT
GAGGTACTCTTCCCCCCGAAACCCAACCTCAAGTCAAGAATTGGGTGCAC
GGCAAAGCAGTCAACTCCCCGTGAGACCACAGTAGGTCTGGTTTCGCGAG
GTATATATACTGTCAAGCATCATCGCCTTGCTGGTTGGTGGTTTCAGAAG
GTTTTGTTGAAACTCTTCATAGGAAGGTGATGCTGAGATACTCAAAGATA
CGTGGCTAGAGGAACGTGCGCTCAATGTCGCGTTTCTTGGTTTGTTGTGG
CTGTGAATACTTGAGTACTGTTGGAGTACACAATGATTTTTGCTCTCAGC
ATTGTTATGTACGACACCTGTTTCTTTCATTGCTCCCATGTATCACGTTA
GCTCTCTCATTGGGCATGTATTATATTTCAAAAACATTCATGGAGGTGTT
TGTGGCAACCTCGGTCGACTATTTACGTGGTTCTCAAGTATGCCTCATTC
CGGCAGACCCAAAACCAGTCATAAATGGGGAAATTGTACCGTTTGCCACA
AACCAGGCGACTCGAGCAGGAGACAACCTGCTCGAACGCAGCGGATGTCG
ACAGTGCAGTCACGACACCGGGGGCGTTGGGGCTGCGGCCATCCATCTCG
TGCGCATGGTTGACATCCGGCAGGACCGAGGTGTGAACAATATGTGGACG
GTATTCATCGGCGCGATGTATGTCTCCATTCCTATTATAGTCAGGTATTT
CATGTATTCGCTTGGGTCGATTGCCAAACCCTGCGTCGAGGAAAGACTGT
GTTTATAACGGACACACCGAGTTTGCCGTGCTCTTGCACGTTCTTCAGTA
CAACGTACGTGCGTGCATTTTCGTGTGTTGTCGTGAATTATGGGTGCAAA
GAACGAAATGATACCTTTGCATTCAACTGCATGTCATCATACCCCAGTCC
ACGTCGGTTTCGCCCGGTTTAGTCACGCCACCCTGCGATCTTGTGGACCT
ACTTCGTGGCGAGAACAACGGGGTTGGAGAAGGAAAGATCGATATGGGCT
GACCTATTTCCACCCTCCCATGTTTCACCACAGACGTGATTGCCATCGAG
GTCAGGGCGAGG
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig8732:4052..5563+

>mRNA_E_fasciculatus_S2_contig8732.16839.1 ID=mRNA_E_fasciculatus_S2_contig8732.16839.1|Name=mRNA_E_fasciculatus_S2_contig8732.16839.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=402bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig8732:4052..5563+ (Ectocarpus fasciculatus EfasUO2)
GTGCTTGTGTATGGAGGTCTCACCGACCAAGGCGAACGCGGGACATTTGC
TGTGGTTCTGGAATATTCGCTCGACGACAACGAACTTGTCGCTCAGATAT
ATGGCGATATCAGCACTCCAGCTCCGTGGGTGGCCCTTTCTCACGTAGCG
TCTGCCGTGAGGTTGATGCTGCTGGACTTTCCCGGTTTACGCTCGAGAGG
CTCTCTGAACTGTCCTGAGCATGGCGACGCAATGCTTTTGGCCGACAAGG
CGACTCGAGCAGGAGACAACCTGCTCGAACGCAGCGGATGTCGACAGTGC
AGTCACGACACCGGGGGCGTTGGGGCTGCGGCCATCCATCTCGTGCGCAT
GGTTGACATCCGGCAGGACCGAGACGTGATTGCCATCGAGGTCAGGGCGA
GG
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