mRNA_E_fasciculatus_S2_contig7416.15591.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_E_fasciculatus_S2_contig7416.15591.1
Unique Name	mRNA_E_fasciculatus_S2_contig7416.15591.1
Type	mRNA
Organism	Ectocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)

Homology

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: JmjC (domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYA0_ECTSI)

HSP 1 Score: 323 bits (827), Expect = 1.230e-106
Identity = 174/184 (94.57%), Postives = 178/184 (96.74%), Query Frame = 1

Query:    1 MRPSGGAFAVGLASLGLASTHAFTGPALTTARSGSSSSSRSRVVASTAVSDSAVAPTAASAADEQYSPPQLVAALREASAQSPPLGVEGVFSAMARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSALKEKSGT 552
            MRPS GAFAVGLASLG ASTHAFTGPALTTARSG SSSSR RVVASTAVSD+AVAP AASAADEQYSPPQLVAALRE SAQSPPLGVEG+FSAMARDDVF+NELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSF+DAKLRFDDVKSALKEKSGT
Sbjct:    1 MRPSRGAFAVGLASLGFASTHAFTGPALTTARSGCSSSSRWRVVASTAVSDTAVAPAAASAADEQYSPPQLVAALRETSAQSPPLGVEGIFSAMARDDVFVNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFEDAKLRFDDVKSALKEKSGT 184

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JGT6_9PHAE)

HSP 1 Score: 183 bits (465), Expect = 9.940e-53
Identity = 88/91 (96.70%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  280 MARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSALKEKSGT 552
            MARDDVF+NELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSF+DAKLRFDDVK+ALKEKSGT
Sbjct:    1 MARDDVFVNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFEDAKLRFDDVKNALKEKSGT 91

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase (Fragment) n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3WNN0_EMIHU)

HSP 1 Score: 92.4 bits (228), Expect = 1.230e-19
Identity = 43/88 (48.86%), Postives = 60/88 (68.18%), Query Frame = 1

Query:  271 FSAMARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSAL 534
            F  +ARD  F+ ++W +KP      +  +AG +TLDDV++AVDSDF+EAG G       GW+MAPVS PRG S++DAKLR+ DV + +
Sbjct:   82 FEVLARDADFVADVWARKPLLVAEPIAGVAGSFTLDDVREAVDSDFLEAGLGV-PTAEGGWKMAPVSQPRGSSYEDAKLRYVDVAAGI 168

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: JmjC (domain-containing protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=R1BJP6_EMIHU)

HSP 1 Score: 92.4 bits (228), Expect = 2.780e-19
Identity = 43/88 (48.86%), Postives = 60/88 (68.18%), Query Frame = 1

Query:  271 FSAMARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSAL 534
            F  +ARD  F+ ++W +KP      +  +AG +TLDDV++AVDSDF+EAG G       GW+MAPVS PRG S++DAKLR+ DV + +
Sbjct:   62 FEVLARDADFVADVWARKPLLVAEPIAGVAGSFTLDDVREAVDSDFLEAGLGV-PTAEGGWKMAPVSQPRGSSYEDAKLRYVDVAAGI 148

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2CTT2_9EUKA)

HSP 1 Score: 85.5 bits (210), Expect = 2.000e-17
Identity = 42/89 (47.19%), Postives = 61/89 (68.54%), Query Frame = 1

Query:  271 FSAMARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSALK 537
            F ++AR   F  +++ ++P      +P +A  +TLD +++AVDSDF+EAGRG    G  GW+MAPVS PRG SF+DAK+R+ DV  A+K
Sbjct:   27 FLSLARSTEFARDIFARQPMLY-GGIPGVADSFTLDHLREAVDSDFLEAGRGIPDNG--GWKMAPVSKPRGSSFEDAKMRYVDVVEAMK 112

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TSI8_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 2.350e-17
Identity = 49/113 (43.36%), Postives = 72/113 (63.72%), Query Frame = 1

Query:  223 LREASAQSPPLGVEGVFSAM-ARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGST--GWRMAPVSTPRGKSFDDAKLRFDDVKSALKEKSGT 552
            L EA      L ++ VF A+ A    FL + WQ+ P F   S+P LA  +T+ D+++A+  +F+ AGRG F + S   GW+MA V   RG SF++AKLR +DV++ALK++SGT
Sbjct:   81 LVEADTGCDKLDLKRVFEALTAPSSPFLAKYWQRAPCFTSTSIPCLAQAWTMADMEEAIAKEFLLAGRGFFEENSETGGWKMAAVGLARGSSFEEAKLRPEDVRAALKQRSGT 193

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase n=2 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XYX3_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 3.580e-17
Identity = 44/107 (41.12%), Postives = 68/107 (63.55%), Query Frame = 1

Query:  235 SAQSPPLGVEGVFSAMARDDVFLNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQ-GSTGWRMAPVSTPRGKSFDDAKLRFDDVKSALKEKSGT 552
            SAQ+ PL V   F A+A    F  ++WQ++PF      P++ G YT+ D+++AV+ DF+EAGRG   + G  GW+MA V   +GK+    K++ +DV+ AL ++SGT
Sbjct:   82 SAQATPLDVTAAFRALANTAQFGQDVWQQEPFVFSEGFPNMVGAYTMLDIEEAVEEDFLEAGRGLVDEDGRAGWQMARVG--KGKASSPKKMKVEDVRKALSQRSGT 186

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1YZE1_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 9.410e-9
Identity = 27/66 (40.91%), Postives = 48/66 (72.73%), Query Frame = 1

Query:  337 DASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSAL 534
            + S  ++AG +T DD+ +A++ DF++A RG+ +    GW++  VSTPRG SF+DA++  ++V++AL
Sbjct:  167 EESASTIAGYFTFDDLAKALEEDFLDADRGS-TDNKKGWKVTAVSTPRGSSFEDARMTLEEVEAAL 231

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BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase (Fragment) n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2MKV5_9STRA)

HSP 1 Score: 62.8 bits (151), Expect = 2.180e-8
Identity = 28/66 (42.42%), Postives = 45/66 (68.18%), Query Frame = 1

Query:  337 DASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSAL 534
            D +L  L G +T DD+  A++ DF++A RG+ +    GW++  VSTPRG SF+DA++   +V++AL
Sbjct:   51 DETLSPLKGYFTYDDLATALEEDFLDADRGS-TDNKKGWKVTSVSTPRGSSFEDARMTLSEVETAL 115

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Match: Bifunctional (lysine-specific demethylase and histidyl-hydroxylase n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XAD1_DIALT)

HSP 1 Score: 62.8 bits (151), Expect = 3.010e-8
Identity = 29/75 (38.67%), Postives = 48/75 (64.00%), Query Frame = 1

Query:  313 WQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTF-SQGSTGWRMAPVSTPRGKSFDDAKLRFDDVKSAL 534
            WQ+ P    A  P   G +T+  +  AV+ +F++AG G + ++G  GW+M  V TPRG SF++AKL + +V++A+
Sbjct:   74 WQRAPLVTRALAPLADGYFTVAHLAAAVERNFLDAGLGVYDARGRAGWQMGRVGTPRGASFEEAKLTWAEVEAAM 148

The following BLAST results are available for this feature:

BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.

BLAST of mRNA_E_fasciculatus_S2_contig7416.15591.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 15


Match Name	E-value	Identity	Description
JmjC	1.230e-106	94.57	domain-containing protein n=1 Tax=Ectocarpus silic...	[more]
Bifunctional	9.940e-53	96.70	lysine-specific demethylase and histidyl-hydroxyla...	[more]
Bifunctional	1.230e-19	48.86	lysine-specific demethylase and histidyl-hydroxyla...	[more]
JmjC	2.780e-19	48.86	domain-containing protein n=1 Tax=Emiliania huxley...	[more]
Hypothetical	2.000e-17	47.19	protein (Fragment) n=1 Tax=Haptolina brevifila Tax...	[more]
Bifunctional	2.350e-17	43.36	lysine-specific demethylase and histidyl-hydroxyla...	[more]
Bifunctional	3.580e-17	41.12	lysine-specific demethylase and histidyl-hydroxyla...	[more]
Bifunctional	9.410e-9	40.91	lysine-specific demethylase and histidyl-hydroxyla...	[more]
Bifunctional	2.180e-8	42.42	lysine-specific demethylase and histidyl-hydroxyla...	[more]
Bifunctional	3.010e-8	38.67	lysine-specific demethylase and histidyl-hydroxyla...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E_fasciculatus_S2_contig7416	contig	E_fasciculatus_S2_contig7416:462..2916 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90	2022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO2	2022-07-07

Properties


Property Name	Value
Taxonomic scope	Eukaryota
Stop	0
Start	1
Seed ortholog score	326.6
Seed ortholog evalue	7.3e-87
Seed eggNOG ortholog	2880.D7FYA0
Model size	552
Hectar predicted targeting category	chloroplast
Exons	4
EggNOG free text desc.	Cupin superfamily protein
EggNOG OGs	2CYFP@1,2S42U@2759
Ec32 ortholog description	Secreted cupin superfamily protein
Ec32 ortholog	Ec-24_003250.1
Cds size	552
COG Functional cat.	S
Best tax level	Eukaryota
Best eggNOG OG	NA\|NA\|NA

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696842404.5023162-CDS-E_fasciculatus_S2_contig7416:461..599	1696842404.5023162-CDS-E_fasciculatus_S2_contig7416:461..599	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7416 462..599 -
1696842404.5153644-CDS-E_fasciculatus_S2_contig7416:821..926	1696842404.5153644-CDS-E_fasciculatus_S2_contig7416:821..926	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7416 822..926 -
1696842404.5253623-CDS-E_fasciculatus_S2_contig7416:1680..1826	1696842404.5253623-CDS-E_fasciculatus_S2_contig7416:1680..1826	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7416 1681..1826 -
1696842404.5354319-CDS-E_fasciculatus_S2_contig7416:2753..2916	1696842404.5354319-CDS-E_fasciculatus_S2_contig7416:2753..2916	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7416 2754..2916 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E_fasciculatus_S2_contig7416.15591.1	prot_E_fasciculatus_S2_contig7416.15591.1	Ectocarpus fasciculatus EfasUO2	polypeptide	E_fasciculatus_S2_contig7416 462..2916 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E_fasciculatus_S2_contig7416:462..2916-
coding sequence from alignment at E_fasciculatus_S2_contig7416:462..2916-

protein sequence of mRNA_E_fasciculatus_S2_contig7416.15591.1

>prot_E_fasciculatus_S2_contig7416.15591.1 ID=prot_E_fasciculatus_S2_contig7416.15591.1|Name=mRNA_E_fasciculatus_S2_contig7416.15591.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=184bp
MRPSGGAFAVGLASLGLASTHAFTGPALTTARSGSSSSSRSRVVASTAVS
DSAVAPTAASAADEQYSPPQLVAALREASAQSPPLGVEGVFSAMARDDVF
LNELWQKKPFFCDASLPSLAGQYTLDDVQQAVDSDFVEAGRGTFSQGSTG
WRMAPVSTPRGKSFDDAKLRFDDVKSALKEKSGT

mRNA from alignment at E_fasciculatus_S2_contig7416:462..2916-

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E_fasciculatus_S2_contig7416.15591.1 ID=mRNA_E_fasciculatus_S2_contig7416.15591.1|Name=mRNA_E_fasciculatus_S2_contig7416.15591.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2455bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7416:462..2916- (Ectocarpus fasciculatus EfasUO2)
ATGAGGCCGTCTGGAGGTGCTTTCGCGGTGGGCCTAGCAAGCCTGGGCTT
GGCCAGCACTCACGCATTCACGGGACCAGCGCTGACCACCGCTCGCAGCG
GCAGTAGCAGCAGCAGCAGGTCGCGCGTTGTTGCTTCAACTGCAGTCAGC
GATTCAGCGGTCGGTGAGAGGGTCGGTGGAGATGATGATCAGCTGCAGTA
ACCGTATCATGTGTCGCTCACGTGAGGGGTGGGTGTTTGTCCTTTTGTGG
CCACTGCTGTAGATGTGGCTGAGAGTTGGTATTGTAGCCGCAAACCGAGG
AGTGCGTTGTGCCGGGTGTGGCTTGTTCGGCGGGTGAAGTTTAACTTTCA
CAACGACAACAACGGCAGCGCCTGCCCCTACCGCAGCAACTACCGCAGTT
GTAGTCTACTGCTGTTACCAGCAGGTCATCTCTGCCAAGAGTTGAGAGGC
ACAGCAGTAGGCAGGTCGTCGTCGTTGAGGTGATGGGGTTTCGAGTTTGG
GTACGCTTCAAAGTGTTTGCTGCCGCAACGCTACACGTACGAGCGAAACA
TGGCCGGCCCCCCGGTTCGGAGATGACGCCAAGCAGATCGCGTTCCCTAC
ACGAGTCGTACCATGATATTGTAACATGCGCCGTTCTACGGAAGGACGGG
TCTGCACCTCTGCACCTACCACTGCTGTTGGTTGCCGCGCACATCGACTG
ACTCCTCAGGGTGTGCATATAAAACCCCTTTGTCGGTGTGCTATTTGGGA
TGCAGCTGCGGTGTGAAATATTAATATTCATCTGCTGCGATACCAAGTCT
TCAACAAAGCACTTGCGGTGTTCGACTCTGTATGTATGCATAGAACGGAG
CATACCGCAACACGCGGTACTACAGTACTGCTGTTCCGCATGAACATGTA
CCTCCGGGTTTGCGGATCGCGGAACACCCGTGAGACGTCTCGGATATTTA
CAGGTTACCGTCACCCCCCGTAGGCACGCGACACGCACACCAACCCTAGG
GCCAATTGCACGCCCGCTCCCGATGAAAAAAAAAAAAAAAACGCGCCATC
ACACAACAACCCCCCCCCCCCGTTGACGTTGAACCGGCAGCACCGACTGC
TGCTTCTGCCGCGGATGAGCAGTACTCTCCGCCGCAGCTGGTGGCAGCGT
TGCGAGAGGCGTCAGCGCAGTCGCCGCCGCTGGGCGTGGAAGGGGTATTC
TCCGCGATGGCCCGAGACGACGTGTTCCTGAACGAGGTGCGCTGTTTCCC
CCAACGGCCACATTGATTGGTAAATTACGGTGTACCGTTCCATGTTTTCC
GCGATGGCCCGGGACGACGGCTTTCTGAATGAGGTACGTTGTTACCGCAA
CAACCACATTTGATTTGGTGAATTTCCGTGTACCATACCACTAGAGGTAT
CATACCCACGAAGGATGCCACCCACCCCCTACAGAACCACCACGTTTTTG
CGGCTCAACATGGGGCCCAAACGCACAAGCACCCTCAGGATCGAGTATAT
TAGGACGGCACAGGATACCACACCTCGCACCACGTAACACCGCACCAGGT
GCAAGTTAGTACTGTTACACGCCACAACCGTTATCGGCAGATGTGATGTG
TGTATCTTGTGCGGTCAAGTTACAATGGATGGCGGCAGAACATCTGTCGC
TGATCCGGCATACCCTCCTCCAGGTCATCACCTACCCACTTGCCGTCCTC
ACCCACACACCCACCCTGCCAAAATTCGCCTCGTCATGTACTTTTCACTC
CCCCCCCTCCATATATCCCTGCCTCTCTCACCGTCTTTCCGTCTCCCCTC
GCCCCAACCTTTCGCAGAGTAGGAAGGAAGATTGGCCATCGATGATCGGT
CCTGTGGGAACAATGCTCCGCCCTCTCGAGGACGTGTGGTCAGTAGTCTA
TCCGCTACAACATGAGTAACACCCCCCCCCGCTCTTTGCCAAAACTCGCG
TGCCTCTTGGGCAACCAATCAACGTGCGAACGGAGACCAGCTGTGGCAGA
AGAAACCCTTCTTCTGCGACGCGAGCCTTCCCAGCCTGGCCGGGCAGTAC
ACCCTCGACGACGTCCAGCAGGCGGTCGACTCGGACTTCGTTGAGGTGGG
GGGTTTACACCTGAATGTTCGGAGCGGTGTACTAACGTATTCCATATGTC
TTAGTCGCTAGACCATTGACCTACTCGCATGCCGGGACGGAGCGATATGC
CGGGGTTTCGCCAGCCCAGGGTACGCTTTTTAAGGTCGATTCAGCAGCTT
GCCGTCTGTTGGATGACCAGAGGATGGTGCTTTCGTGCTTGCGCTCGCCG
TGGGTGCTCCGAAACAGGCCGGGAGAGGGACTTTTAGTCAGGGTTCGACA
GGGTGGAGGATGGCGCCGGTGTCTACGCCGAGGGGGAAGAGCTTCGACGA
CGCCAAGCTGAGGTTCGACGACGTCAAGAGCGCGTTGAAAGAGAAGAGCG
GGACT

Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7416:462..2916-

>mRNA_E_fasciculatus_S2_contig7416.15591.1 ID=mRNA_E_fasciculatus_S2_contig7416.15591.1|Name=mRNA_E_fasciculatus_S2_contig7416.15591.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=552bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7416:462..2916- (Ectocarpus fasciculatus EfasUO2)
ATGAGGCCGTCTGGAGGTGCTTTCGCGGTGGGCCTAGCAAGCCTGGGCTT
GGCCAGCACTCACGCATTCACGGGACCAGCGCTGACCACCGCTCGCAGCG
GCAGTAGCAGCAGCAGCAGGTCGCGCGTTGTTGCTTCAACTGCAGTCAGC
GATTCAGCGGTCGCACCGACTGCTGCTTCTGCCGCGGATGAGCAGTACTC
TCCGCCGCAGCTGGTGGCAGCGTTGCGAGAGGCGTCAGCGCAGTCGCCGC
CGCTGGGCGTGGAAGGGGTATTCTCCGCGATGGCCCGAGACGACGTGTTC
CTGAACGAGCTGTGGCAGAAGAAACCCTTCTTCTGCGACGCGAGCCTTCC
CAGCCTGGCCGGGCAGTACACCCTCGACGACGTCCAGCAGGCGGTCGACT
CGGACTTCGTTGAGGCCGGGAGAGGGACTTTTAGTCAGGGTTCGACAGGG
TGGAGGATGGCGCCGGTGTCTACGCCGAGGGGGAAGAGCTTCGACGACGC
CAAGCTGAGGTTCGACGACGTCAAGAGCGCGTTGAAAGAGAAGAGCGGGA
CT