mRNA_E_fasciculatus_S2_contig7257.15449.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig7257.15449.1
Unique NamemRNA_E_fasciculatus_S2_contig7257.15449.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Rhomboid (domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G3N4_ECTSI)

HSP 1 Score: 506 bits (1304), Expect = 1.350e-174
Identity = 248/278 (89.21%), Postives = 259/278 (93.17%), Query Frame = 1 
Query:    4 MAISSLSRRQRVTVLLGAAAVAVLSRAYFVEVWKGVVLRGAGDTDANGYYKWEPTERSYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKANDKKQQQSRVWFVMRSDEPEYRNPTAASILDNTPPSTGWAVGGPRKDVGGGLRPPPEVHVCGKRVTPDADPAEQRSGGGGGRRGRSAFELGRGPAEVVSYPGTVLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFRYLYL 837
            MAISSLSRRQRVT LLGA+AVA+LS AYFVEVWKGVV+RG GDTDANGYYKWEPT++SYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKA DK QQQSRVWFVMRS EPEYR+P A S+LDNTPP+TGWAVG P+KD G GL PPPEVHVCGKRV PDADPAEQ   GGGGRRG+S FELGRGPAE+VSYPGTVLLLVINLAFAYHLWA RVSPDAVAISYA FWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFRYLYL
Sbjct:    1 MAISSLSRRQRVTALLGASAVAILSWAYFVEVWKGVVVRGGGDTDANGYYKWEPTQKSYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKAIDK-QQQSRVWFVMRSGEPEYRSPNALSVLDNTPPTTGWAVGSPQKDAGDGLGPPPEVHVCGKRVAPDADPAEQWKRGGGGRRGKSPFELGRGPAEIVSYPGTVLLLVINLAFAYHLWANRVSPDAVAISYARFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFRYLYL 277
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Rhomboid (domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKD5_9STRA)

HSP 1 Score: 123 bits (308), Expect = 1.080e-28
Identity = 85/251 (33.86%), Postives = 130/251 (51.79%), Query Frame = 1 
Query:   85 YFVEVWKGVVLRGAGDTDANGYYKW----EPTERSYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKANDKKQQQSRVWFVMRSDEPEYRNPTAASILDNTPPSTGWAVGGPRKDVGGGLRPPPEVHVCGKRVTPDADPAEQRSGGGGGRRGRSAFELGRGPAEVVSYPGTVLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFR 825
            Y   +W+GVV++GA +   +GYY +    EPT+  Y+++A+      R+P+V+ L +  P    +    Y L+ +++      +   + S    Y +   +      PP TGW     R    G    PP +    +    +  P E R     GRRG    + G G A + + PGT +LL +N+A A  LW +RV P+AVA+SYA   ++++YWR  TASFSHFEPLHL FN M  +N+  LE+  G F+
Sbjct:   28 YHAGLWRGVVVQGARNPALDGYYHYNFADEPTQPMYVQNARRLGP--RLPDVFGLSALTPVTAGEDAKQYLLQQDNRGHWGLGLRGALTS---HYVSTDPSPPGTEAPPQTGWVA--VRARASGA---PPIIRAVWRA---EPAPKEWR-----GRRGGWEGD-GGGLAALAAMPGTAVLLAVNVAAALWLWVRRVPPEAVAVSYAAVVQDKQYWRCVTASFSHFEPLHLGFNMMSLYNSGPLEQDTGNFK 259
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XIE2_HETAK)

HSP 1 Score: 91.3 bits (225), Expect = 3.610e-17
Identity = 79/292 (27.05%), Postives = 132/292 (45.21%), Query Frame = 1 
Query:    1 HMAISSLSRRQRVTVLLGAAAVAVLSRAYFVEVWKGVVLRGAGDTDANGYYKWEPTERSYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKANDKKQQQSRVWFVMRSDEPEYRNPTAASILDNT------PPSTGWAVGGPR------KDVGGGLRPPPEVHVCGKRVTPDADPAEQRSGGGGGRRGRSAFELGRGPAEVVSYPGTVLLLVINLAFAYHLWAKRVSP-DAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFRYLYL 837
            H  I  L  +  + V+LG   +   +   F +    VV R  G++   G Y    +  ++  S Q     + +P++Y++  +G     + G +    A D+K     +W++      +    T A            PP  GW    PR      K++   + P P +   G  +  + + A      GG  +G +  +  R   E+++ P T+ LL++N A  Y LWA RV P D V+ S   F EE+EYWR  TASFSHF+ +H+ FN +  +N + +E + G + +L L
Sbjct:    5 HSLIGMLRSKTSILVILGTTLLFYFAARTFDDKSYLVVTRPEGNSPITGIYFASVSSGAFAWSLQQDKKTY-LPDIYEISYKGKDGLAEPGTIQL--ARDRK-----LWYIYERFPGDQTKKTVALFTAEAGQSAFYPPKAGWRAV-PRDNTREAKNLAAAV-PGPIITYGGPTLLWELEVA------GGSNKGSNKQKNIR---ELLATPVTLFLLIVNAAAGYKLWADRVLPVDRVSFSVRAFVEEQEYWRAITASFSHFDLMHIGFNLLSLYNLKAVETVYGPWHHLCL 277
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RP04_9STRA)

HSP 1 Score: 79.0 bits (193), Expect = 4.830e-15
Identity = 37/79 (46.84%), Postives = 54/79 (68.35%), Query Frame = 1 
Query:  583 VVSYPGTVLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGT 819
            ++S PGT L+L +NL  A+ L+AK V  + VA+SY  F   +EYWR +TASF+H++ +HL+FN M  +N   LE + GT
Sbjct:   24 LISRPGTSLMLGLNLGAAWWLYAKSVPVERVAVSYEKFVVSKEYWRAWTASFAHYDIMHLLFNTMSLYNLGALEDIYGT 102
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Hypothetical (protein n=2 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XQ68_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 6.350e-11
Identity = 43/113 (38.05%), Postives = 61/113 (53.98%), Query Frame = 1 
Query:  505 PAEQRSGGGGGRRGRS--AFELGRGPAEVVSYPGTVLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNTRELERLLGTFRYLYL 837
            PAE   GGG  RR R   +   G G ++ ++ P T   L++N   A+ L+  RV  + VA +Y       EYWR  TA+FSH+EP+H +FN    +  + LE LLG+    YL
Sbjct:  132 PAEVTCGGGALRRVRQLRSGRTGEGVSQAMAAPVTCAFLLLNAVVAFLLYRWRVPWEDVAFNYEAVVGGGEYWRAATAAFSHYEPMHFLFNMSSLYAMQGLEALLGSVSLFYL 244
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BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Match: Rhomboid (domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RQF1_THAOC)

HSP 1 Score: 67.0 bits (162), Expect = 7.690e-9
Identity = 32/85 (37.65%), Postives = 53/85 (62.35%), Query Frame = 1 
Query:  583 VVSYPGTVLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEPLHLVFNAMGTWNT-RELE-RLLGTFRYL 831
            V+ +P T +++++N+A A+H W +RV P +V+  YA    E E+WR  T + +HF+ LH+ FN +   N  RE+E  LL +  +L
Sbjct:  244 VIDHPATCVIILVNVALAFHYWNRRVDPSSVSKQYARIVHEHEWWRGITGATAHFDLLHVGFNMVSMDNLGREIEGTLLNSIEFL 328
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig7257.15449.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
Rhomboid1.350e-17489.21domain-containing protein n=2 Tax=Ectocarpus TaxID... [more]
Rhomboid1.080e-2833.86domain-containing protein n=1 Tax=Tribonema minus ... [more]
Hypothetical3.610e-1727.05protein (Fragment) n=1 Tax=Heterosigma akashiwo Ta... [more]
Hypothetical4.830e-1546.84protein (Fragment) n=1 Tax=Rhizochromulina marina ... [more]
Hypothetical6.350e-1138.05protein n=2 Tax=Phaeomonas parva TaxID=124430 RepI... [more]
Rhomboid7.690e-937.65domain-containing protein n=1 Tax=Thalassiosira oc... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig7257contigE_fasciculatus_S2_contig7257:3900..7237 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score505.4
Seed ortholog evalue1.7e-140
Seed eggNOG ortholog2880.D7G3N4
Model size837
KEGG koko:K09651
Hectar predicted targeting categorysignal peptide
Exons5
EggNOG free text desc.serine-type endopeptidase activity
EggNOG OGsKOG2632@1,KOG2632@2759
Cds size834
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01002
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig7257.15449.1prot_E_fasciculatus_S2_contig7257.15449.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig7257 3903..7237 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842390.125665-UTR-E_fasciculatus_S2_contig7257:3899..39021696842390.125665-UTR-E_fasciculatus_S2_contig7257:3899..3902Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig7257 3900..3902 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842390.1414063-CDS-E_fasciculatus_S2_contig7257:3902..40401696842390.1414063-CDS-E_fasciculatus_S2_contig7257:3902..4040Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7257 3903..4040 +
1696842390.1511753-CDS-E_fasciculatus_S2_contig7257:4473..45691696842390.1511753-CDS-E_fasciculatus_S2_contig7257:4473..4569Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7257 4474..4569 +
1696842390.1613147-CDS-E_fasciculatus_S2_contig7257:5369..55381696842390.1613147-CDS-E_fasciculatus_S2_contig7257:5369..5538Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7257 5370..5538 +
1696842390.1727617-CDS-E_fasciculatus_S2_contig7257:6245..64961696842390.1727617-CDS-E_fasciculatus_S2_contig7257:6245..6496Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7257 6246..6496 +
1696842390.1832933-CDS-E_fasciculatus_S2_contig7257:7057..72371696842390.1832933-CDS-E_fasciculatus_S2_contig7257:7057..7237Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7257 7058..7237 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig7257.15449.1

>prot_E_fasciculatus_S2_contig7257.15449.1 ID=prot_E_fasciculatus_S2_contig7257.15449.1|Name=mRNA_E_fasciculatus_S2_contig7257.15449.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=278bp
MAISSLSRRQRVTVLLGAAAVAVLSRAYFVEVWKGVVLRGAGDTDANGYY
KWEPTERSYIKSAQAPAGAWRVPNVYDLVSRGPQFRKDAGPLYFLKANDK
KQQQSRVWFVMRSDEPEYRNPTAASILDNTPPSTGWAVGGPRKDVGGGLR
PPPEVHVCGKRVTPDADPAEQRSGGGGGRRGRSAFELGRGPAEVVSYPGT
VLLLVINLAFAYHLWAKRVSPDAVAISYALFWEEREYWRLFTASFSHFEP
LHLVFNAMGTWNTRELERLLGTFRYLYL
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mRNA from alignment at E_fasciculatus_S2_contig7257:3900..7237+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig7257.15449.1 ID=mRNA_E_fasciculatus_S2_contig7257.15449.1|Name=mRNA_E_fasciculatus_S2_contig7257.15449.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=3338bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7257:3900..7237+ (Ectocarpus fasciculatus EfasUO2)
CACATGGCCATCTCTTCACTGTCGAGACGCCAGCGGGTGACCGTGCTCCT
TGGAGCCGCTGCCGTGGCGGTACTGTCCCGGGCTTACTTCGTTGAAGTTT
GGAAGGGGGTAGTGCTTCGAGGTGCCGGAGACACAGATGCGGTGAGATAG
TATTTTGTGCGGAGTAAGTATTTGTACATATCGAAGGAAGTACATATGCA
CGATATGTTTTGTTCGCAGCATCTAGGATCTCCCTTGGCATAGGTAGATG
CGCCGAGGAGCTTCTTGCCTGAGCTTACTGCGAGCTGTGACGAGGTACAT
ACTGCATTTGTCACCGAGCAGGATTTCTGACTACATATTTTCCTTTAAGT
GAGGCCCCTAGAGGAGATGGACAAGACCCGTTAAATGGCAAAGTGAGACT
GGCAGAGCGGAGGCCCCGCAGTCGCGATGGTTTGAGTCACCCACTCGACG
AGGATTTGGCGGAACCCCTTAACTGTCCTCTTCCACAACATGAAAAGAAG
CTGCAGCGGTATGTATACTCACGCGCCCCCACACGTGGACAATATTTGAC
ATGTTTGGCGATCGGTCTGGTCAGAATGGCTACTACAAATGGGAGCCGAC
GGAGAGATCGTACATCAAGAGCGCTCAAGCACCGGCGGGGGCGTGGCGAG
TCCCGAATGTCTACGATTTGGTGAGAGCTCGCGCGACACTGCTGTTGTTC
TTTTTCGTCTCGTTCCATCACTTGACAGTAAGTCAGCCCTCGCACACAGC
GTTTCATTTCCGGGTAGGAGCTCCAATCCGTAGTCACGCATAAGAGTTCC
TCCCCCTGACGCAGAACGTCTAGATACGATTTGGTGACAGCTTGTGACAG
CTTGGGCGGCCCTTCCTTGCCTGTTTCTGTAATTTTCTGCCACTGGGCAA
TATTGAAACTGGTAGCATGCATCGGTGCTCTAGTTGACCACGCCGTGACG
AATGGCGTCTGCCCGAAGAGAAGCGGCAAACCTCTAAATAGTGCGATCAC
CAATCCCAGAGGTCGGGTGTTGTGTAGTGAGGGGACGGGGAAGCACTGCT
GTTGAGTGTTGAGGCGATCGGGAGGACTCGAACATACTTCACCCGAGGAA
CATTCTCTGCACGCGCGAAAATTCCGTCCCGTCCCCTAGAAAGGGAACTC
AGGGACAACATGAGAACGAAGTTCTGCCGCATTTTGTATCCACCCGAACA
GAACGAAGTCCGGCCGCGTTTTTCTCCTCCCGGACAGAACGAAGCTCGCC
GCGTTTTGCCCGGACAAACACAATCCCGCCGCGTTTTGTTCTCGGCTTGA
ACAGAACGAAATACCGCCGCGTTCTTTCTCCTCCTCGTTCTTTCTCATCC
TCGAAGAGAACGTAGTCCGACCGCGTTTCTTCTCCGCCCGAAACAAAACC
AATCCCGCCGCGTTTATTTCTCCGCCCGAAATAAACGAAATCCCGCCCCG
TTGTTTCTCCGCCCGAACAGGTATCTCGAGGGCCGCAGTTCCGGAAGGAC
GCCGGGCCGCTCTACTTCTTGAAGGCCAATGACAAGAAACAGCAGCAGTC
GCGGGTGTGGTTCGTGATGCGATCGGACGAACCGGAGTACCGAAACCCGA
CCGCCGCTTCCATTCTGGACAACACCCCTCCGTCTACTGGTAAACCCTCC
CCCCCGCGCGCCAATGCCCCGCAGTCAACCCTTGAACGATTATTCTGTTG
CGACGGTGCTTGGGCTGTTGAATACCGTAGGTGGCACAAGGTGCCACGCA
GCCTTGGCCGATTTTTCAAAGCTGGATTAGATTACTCCACGACGAAGGGG
ATGCACGAATGAAACGACACGTTTTCGGCGCTGCCAGTCGTTGTGTTGTG
GAGAAATCGATATTTTGAAAACAGCTTCAGAGGGTGTGGTATCTTGTGTC
ATGTCACGTATGCATACGGTAGTCAAGAGAATCAACGAGCCAGTTGTTGT
CGAAGTTGGTGGTGCTTTTGCCTACACGCAAAAAGCCGTTGCGATACGTG
AGAATCCCCAGTTCTTCCATGTTCTTTGCGCCGACTCTTGCGCCGTGTTC
GTAAGGTTGTTGTCTTCCTCTCGATGTATTTTCTTTCAATATCTCGGGCC
GGGTGTACATCCAATGATATGCCCTCTTCCGTGGTCTCCATAATGTCACC
TAAGGTACCCACCCCTTCCTTCCCACAGCATCGTTGCGTCACCTTTCGCG
GCTAGCCTTTCCCGTCACCACCACCGTTGCATTGCATTGCATTTCCGTCC
GCACCCGCCTCCTTGGCCCATCTTGCGGGACACGACATCACGCCGCCCTT
TGACTTGTCTTCTCTTTCGATACATCCCCGGACACAATGGCCGCAGGCTG
GGCCGTAGGGGGTCCGCGGAAAGACGTAGGGGGCGGCCTGCGCCCACCAC
CGGAGGTACACGTTTGCGGGAAGAGGGTGACGCCGGACGCAGACCCTGCG
GAGCAGCGGAGCGGCGGCGGGGGTGGCAGGAGGGGGAGGAGCGCGTTCGA
GTTGGGCAGGGGGCCTGCCGAGGTGGTGAGCTACCCGGGCACGGTTCTGC
TCCTGGTCATCAACCTCGCCTTCGCGTATCATCTCTGGGCGAAGCGGGTG
AGTGAGGTCGGGGATGGACCGGACTGCCGTACATAGGCTGTGAATGTATG
CACGTGAGTGTTGATTTGTAGTGCACGCAGTGCTTTTGGTGTTTGCGGAA
AGCCCTTTCAAATGATGAATCCATGTTTTGGCCGCTGTTTTTCTCCTTTG
CGGGCTTTTTTTCGATTCTGTTGTCGGCATGAACAACCTCAAGTCGAAGG
TTGGCTCCGTTACACTCGCTCGGATCGAGGCTGTACACACACCTCTACAC
CAACATCGAAAATATCCTAACTGAAAACGAAAGAGTACTTGGCACTTGGC
AAAAAGGCGCTGTTCGCTATACACGCTCACGCGTGTCTCAAACGACTACT
GTACTTAGAAAGCCGCTTTCAAACCTCTCTCTCGGACAACAAGCTTCTCG
CCACTCATGCACGCGCCTAAGACGCAACACGCACTCACGCACGTCTCAAA
GAACGACTTCAATAGCCGCCTTCAAGCCTCTCAAGGACAACGACATTTGA
CATTTCCTGCCACCACCACCGCTAACACCGCTGTTGGTTGTTGTCTTGTC
TTGTCAAGGTCTCGCCCGACGCCGTGGCTATCAGCTACGCGCTTTTCTGG
GAGGAGCGCGAGTACTGGCGGCTCTTCACGGCGTCCTTCAGCCACTTCGA
GCCGCTGCACCTGGTGTTCAACGCCATGGGCACGTGGAACACCCGCGAGC
TAGAGCGGCTGCTGGGAACGTTCCGGTACCTGTACTTG
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7257:3900..7237+

>mRNA_E_fasciculatus_S2_contig7257.15449.1 ID=mRNA_E_fasciculatus_S2_contig7257.15449.1|Name=mRNA_E_fasciculatus_S2_contig7257.15449.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=834bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7257:3900..7237+ (Ectocarpus fasciculatus EfasUO2)
ATGGCCATCTCTTCACTGTCGAGACGCCAGCGGGTGACCGTGCTCCTTGG
AGCCGCTGCCGTGGCGGTACTGTCCCGGGCTTACTTCGTTGAAGTTTGGA
AGGGGGTAGTGCTTCGAGGTGCCGGAGACACAGATGCGAATGGCTACTAC
AAATGGGAGCCGACGGAGAGATCGTACATCAAGAGCGCTCAAGCACCGGC
GGGGGCGTGGCGAGTCCCGAATGTCTACGATTTGGTATCTCGAGGGCCGC
AGTTCCGGAAGGACGCCGGGCCGCTCTACTTCTTGAAGGCCAATGACAAG
AAACAGCAGCAGTCGCGGGTGTGGTTCGTGATGCGATCGGACGAACCGGA
GTACCGAAACCCGACCGCCGCTTCCATTCTGGACAACACCCCTCCGTCTA
CTGGCTGGGCCGTAGGGGGTCCGCGGAAAGACGTAGGGGGCGGCCTGCGC
CCACCACCGGAGGTACACGTTTGCGGGAAGAGGGTGACGCCGGACGCAGA
CCCTGCGGAGCAGCGGAGCGGCGGCGGGGGTGGCAGGAGGGGGAGGAGCG
CGTTCGAGTTGGGCAGGGGGCCTGCCGAGGTGGTGAGCTACCCGGGCACG
GTTCTGCTCCTGGTCATCAACCTCGCCTTCGCGTATCATCTCTGGGCGAA
GCGGGTCTCGCCCGACGCCGTGGCTATCAGCTACGCGCTTTTCTGGGAGG
AGCGCGAGTACTGGCGGCTCTTCACGGCGTCCTTCAGCCACTTCGAGCCG
CTGCACCTGGTGTTCAACGCCATGGGCACGTGGAACACCCGCGAGCTAGA
GCGGCTGCTGGGAACGTTCCGGTACCTGTACTTG
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