mRNA_E_fasciculatus_S2_contig7053.15217.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig7053.15217.1
Unique NamemRNA_E_fasciculatus_S2_contig7053.15217.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Similar (to Gram-negative bacteria-binding protein 1, partial n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTS2_ECTSI)

HSP 1 Score: 295 bits (754), Expect = 9.080e-98
Identity = 186/246 (75.61%), Postives = 201/246 (81.71%), Query Frame = 1 
Query:    1 QGEGLTNGQQ-ARNLKKMKT-KKVKAKASTDHEYLGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMFEYQEVESSSTTTGMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGTAEYDDDEGYGSSIE-SVCSNGVPGIESNGVCCETSCXXXXGAGCSSVGNGAESCCTSTILEFGEVCGSAPCVI 729
            QGEG+ NGQQ AR+LK+MK  KKVKA+ASTDHEYLGCYRYNK+ G LLK+GKSFFD TDQALCRAHCEEYDTAFYA+E GD CWCPTSPDINDYIIDE G CDKPCTGDPSIFCGG  SISMFEYQ+V+SS T+TG       XXXXXXXXXXXXXXXXXXXXXXXXXXX          G+ SS E SVC+NGV GIES+GVCCE SC    G+GCS  GNGA SCCTSTILE GEVCG APC+I
Sbjct:   15 QGEGVRNGQQGARSLKRMKREKKVKARASTDHEYLGCYRYNKENGMLLKNGKSFFDKTDQALCRAHCEEYDTAFYALEDGDVCWCPTSPDINDYIIDEVGICDKPCTGDPSIFCGGVSSISMFEYQDVDSS-TSTGEYEEDDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---XGFSSSTEESVCTNGVSGIESDGVCCEISCGQCGGSGCSMRGNGAASCCTSTILEVGEVCGVAPCII 256
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXK6_ECTSI)

HSP 1 Score: 254 bits (649), Expect = 7.080e-78
Identity = 138/205 (67.32%), Postives = 153/205 (74.63%), Query Frame = 1 
Query:    1 QGEGLTNGQQ-ARNLKKMKT-KKVKAKASTDHEYLGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMFEYQEVESSSTTTGMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGTAEYDDDEGYGSSIESVCSNGVPGIESNGVCCE 609
            QGEGL NGQQ ARN ++MK  KKV+AKASTDHEYLGCYRYNKDI  LLK+GKSF+D TDQALCRAHCEEYDTAFYAME+GD CWCPTSPDINDYIID+ G+CDKPCTGDPSIFCGG +SISMFEYQ+V  SST TG      XXXXX                           +  DD+G+ SS ESVC+NGV GIES+GVCCE
Sbjct:   50 QGEGLRNGQQGARNPERMKKEKKVEAKASTDHEYLGCYRYNKDISSLLKNGKSFYDKTDQALCRAHCEEYDTAFYAMEHGDICWCPTSPDINDYIIDDVGNCDKPCTGDPSIFCGGVFSISMFEYQDV-GSSTGTGEYEEYEXXXXX--------------------------VQEXDDDGFSSSTESVCTNGVSGIESDGVCCE 227
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JP22_9PHAE)

HSP 1 Score: 68.6 bits (166), Expect = 1.110e-9
Identity = 31/60 (51.67%), Postives = 38/60 (63.33%), Query Frame = 1 
Query:  559 VCSNGVPGIESNGVCCETSCXXXXGAGCSSVGNGAESCCTSTILEFGEVC---GSAPCVI 729
             CSNG+ G E +G CC   C    G GCS +G GAESCCTS I+  GE C   G+APC++
Sbjct:  399 TCSNGLAGYEKSGACCSVGCGTCGGEGCSQLGQGAESCCTSNIIAAGEPCSEKGAAPCIM 458
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Hypothetical (leucine rich repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FY01_ECTSI)

HSP 1 Score: 67.0 bits (162), Expect = 3.940e-9
Identity = 33/69 (47.83%), Postives = 42/69 (60.87%), Query Frame = 1 
Query:  532 EGYGSSIESVCSNGVPGIESNGVCCETSCXXXXGAGCSSVGNGAESCCTSTILEFGEVC---GSAPCVI 729
            EGY  +    CSNG+ G E++G CC   C    G GCS +G GAESCCTS I+   E C   G+APC++
Sbjct:  373 EGY--TCAQTCSNGLAGYENSGACCSVGCGTCGGEGCSQLGEGAESCCTSNIIAAREPCSEKGAAPCIM 439
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K060_9PHAE)

HSP 1 Score: 63.2 bits (152), Expect = 7.450e-8
Identity = 36/100 (36.00%), Postives = 51/100 (51.00%), Query Frame = 1 
Query:   85 DHEYLGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMFEYQEVES 384
            D  YLGCY    D   +     +  DMT +  C  +C  Y   +Y  +Y   CWC    D  DY  +  G+CD  C+GD S  CGG YS+S++E  +V++
Sbjct:  238 DPSYLGCYADPAD-SRIFDLVATADDMTSEN-CLGNCGAYQ--YYGTQYSTQCWCG---DNADYDANGAGECDMACSGDSSEICGGFYSMSVYENDDVDA 330
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JG29_9PHAE)

HSP 1 Score: 62.4 bits (150), Expect = 1.810e-7
Identity = 29/62 (46.77%), Postives = 39/62 (62.90%), Query Frame = 1 
Query:  559 VCSNGVPGIESNGVCCETSCXXXXGAGCSSVGNG--AESCCTSTILEFGEVCG---SAPCVI 729
            VCSNG+PGI+++  CC   C    G+GC   G G  A++CC S I+E+GE+C     APC I
Sbjct: 1082 VCSNGIPGIQTDNACCLAECGACGGSGCDEFGGGLGADNCCVSDIVEYGELCSIALEAPCYI 1143
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: kremen (protein 1-like n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1DF47F)

HSP 1 Score: 58.2 bits (139), Expect = 4.880e-7
Identity = 32/100 (32.00%), Postives = 48/100 (48.00%), Query Frame = 1 
Query:   73 KASTDHEYLGCYRYNKD--IGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMFE 366
            K + + +YLGCY    D  + G   H  S    T    C   C E    F + +YG  C+C     +  Y    + +C+KPC GDP+  CGGT+  S+++
Sbjct:   60 KCTINFKYLGCYEDRSDRILYGRSMHSSSLTTET----CLTFCFESGFKFASTQYGSHCFCGNV--LRGYPEKAESECNKPCAGDPTEMCGGTWRGSIYK 153
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: Uncharacterized (protein (Fragment) n=1 Tax=Tulasnella calospora MUT 4182 TaxID=1051891 RepID=A0A0C3KZP9_9AGAM)

HSP 1 Score: 58.2 bits (139), Expect = 1.410e-6
Identity = 30/100 (30.00%), Postives = 45/100 (45.00%), Query Frame = 1 
Query:   97 LGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMFEYQEVESSSTT 396
            LGCY        L     S         C+A+C      +  +EYG  C+C  + +    +      C+K C  DPS +CGGT+++++F Y  V  SS T
Sbjct:   30 LGCYTDQNSPRTLSGASYSSTVTMTYDTCKAYCLGKGYNYAGVEYGSQCYCGNALNSPGKLAASQTSCNKACASDPSNYCGGTWTLTVFYYNGVAGSSAT 129
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: xylosyltransferase (oxt-like n=1 Tax=Haliotis rufescens TaxID=6454 RepID=UPI00201F3AAC)

HSP 1 Score: 57.8 bits (138), Expect = 1.770e-6
Identity = 30/94 (31.91%), Postives = 50/94 (53.19%), Query Frame = 1 
Query:   82 TDHEYLGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMF 363
            T  EYLGC++       +L H  S   +T Q +C A+C     A    + G+ C+C  S     +I D++ +C +PC G+ ++ CGGT+  S++
Sbjct:  130 TCKEYLGCFK--DSAARILPHPYSSQSLT-QEVCTAYCTTEGYALAGTQSGNQCFCGNSIPTG-HIRDDENNCQRPCAGNTTLICGGTWYSSVY 219
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BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Match: WSC (domain-containing protein n=1 Tax=Pseudoalteromonas amylolytica TaxID=1859457 RepID=A0A1S1MWX8_9GAMM)

HSP 1 Score: 55.5 bits (132), Expect = 2.510e-6
Identity = 29/96 (30.21%), Postives = 50/96 (52.08%), Query Frame = 1 
Query:   76 ASTDHEYLGCYRYNKDIGGLLKHGKSFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDCDKPCTGDPSIFCGGTYSISMF 363
            A + H Y+GC+  N ++G  L        +T  + C+A C +    +  ++YG  C+C    +  +Y    D DC+ PCTG+ + FCGG +  S++
Sbjct:   29 AQSQH-YIGCFTDNIELGRDLPVRAGLTTVTIDS-CKAACNKASYKYAGLQYGSLCFC--GHNYGNYSQVPDSDCNMPCTGNSTQFCGGLWRNSIY 120
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig7053.15217.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 19
Match NameE-valueIdentityDescription
Similar9.080e-9875.61to Gram-negative bacteria-binding protein 1, parti... [more]
Uncharacterized7.080e-7867.32protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized1.110e-951.67protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Hypothetical3.940e-947.83leucine rich repeat protein n=1 Tax=Ectocarpus sil... [more]
Uncharacterized7.450e-836.00protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized1.810e-746.77protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
kremen4.880e-732.00protein 1-like n=1 Tax=Mercenaria mercenaria TaxID... [more]
Uncharacterized1.410e-630.00protein (Fragment) n=1 Tax=Tulasnella calospora MU... [more]
xylosyltransferase1.770e-631.91oxt-like n=1 Tax=Haliotis rufescens TaxID=6454 Rep... [more]
WSC2.510e-630.21domain-containing protein n=1 Tax=Pseudoalteromona... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig7053contigE_fasciculatus_S2_contig7053:2389..4918 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score295.0
Seed ortholog evalue3.1e-77
Seed eggNOG ortholog2880.D8LTS2
Model size732
Hectar predicted targeting categoryother localisation
Exons6
EggNOG free text desc.WSC domain
EggNOG OGs2EWMG@1,2SYEH@2759
Cds size732
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig7053.15217.1prot_E_fasciculatus_S2_contig7053.15217.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig7053 2389..4918 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842365.644864-CDS-E_fasciculatus_S2_contig7053:2388..24331696842365.644864-CDS-E_fasciculatus_S2_contig7053:2388..2433Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 2389..2433 +
1696842365.6573775-CDS-E_fasciculatus_S2_contig7053:2804..29871696842365.6573775-CDS-E_fasciculatus_S2_contig7053:2804..2987Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 2805..2987 +
1696842365.6674566-CDS-E_fasciculatus_S2_contig7053:3337..34461696842365.6674566-CDS-E_fasciculatus_S2_contig7053:3337..3446Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 3338..3446 +
1696842365.685129-CDS-E_fasciculatus_S2_contig7053:3734..39501696842365.685129-CDS-E_fasciculatus_S2_contig7053:3734..3950Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 3735..3950 +
1696842365.6984944-CDS-E_fasciculatus_S2_contig7053:4141..42021696842365.6984944-CDS-E_fasciculatus_S2_contig7053:4141..4202Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 4142..4202 +
1696842365.7146149-CDS-E_fasciculatus_S2_contig7053:4800..49181696842365.7146149-CDS-E_fasciculatus_S2_contig7053:4800..4918Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7053 4801..4918 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig7053.15217.1

>prot_E_fasciculatus_S2_contig7053.15217.1 ID=prot_E_fasciculatus_S2_contig7053.15217.1|Name=mRNA_E_fasciculatus_S2_contig7053.15217.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=244bp
QGEGLTNGQQARNLKKMKTKKVKAKASTDHEYLGCYRYNKDIGGLLKHGK
SFFDMTDQALCRAHCEEYDTAFYAMEYGDTCWCPTSPDINDYIIDEDGDC
DKPCTGDPSIFCGGTYSISMFEYQEVESSSTTTGMYEQYESPGSTTVIYE
EYKSSSSKTVIYEEYEGSSGTAEYDDDEGYGSSIESVCSNGVPGIESNGV
CCETSCGQCGGAGCSSVGNGAESCCTSTILEFGEVCGSAPCVI*
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mRNA from alignment at E_fasciculatus_S2_contig7053:2389..4918+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig7053.15217.1 ID=mRNA_E_fasciculatus_S2_contig7053.15217.1|Name=mRNA_E_fasciculatus_S2_contig7053.15217.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2530bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7053:2389..4918+ (Ectocarpus fasciculatus EfasUO2)
CAGGGCGAAGGGTTGACGAATGGCCAGCAGGCCCGGAACCTCAAGGTACG
ACTCTTTGAACACTTGTTCTCCCCAAGTACACGAGCACACACACTCAAAA
AGTAAAAACATCCTATTTATGTAGTAAAGTTCCACCAAACGGAGACTGCG
GAGGGCATCGCAGAACCCTTAAAGATAAAAAACATTTTTCGCAGTGCCAG
ATGTCAACACGGATGGTAGATGGTAGATGGGCCACAACAACATGCGATTG
CTGCACATATTTCAAAAGCTGTTTGCATGCCTGTTAGCCGTAAACGTTTT
TCGTTCTTCATTCTCGTGTGCGAATTTTCATCGAATGTAAATTTATGTAT
TAACTGCTGTTGTCTTGTATTGCCATGCTCCCTCGCTTGCGTTTGTTTGG
GGGGTCGTGTCTACAGAAAATGAAAACCAAGAAGGTGAAAGCCAAGGCAT
CCACCGATCACGAGTACCTGGGCTGCTATCGCTACAACAAGGACATTGGT
GGGCTCTTGAAGCACGGAAAATCGTTCTTTGACATGACGGACCAGGCCCT
GTGCCGTGCCCACTGCGAAGAGTACGATACAGCCTTCTATGCCATGGAGG
TATGGATATGGGTTCGATGTGTAACGCCTTCGGCCGTGTCCTGTCCTAGT
GGAGCGTAGTATGAAAACGCAACTATCAGTTGCCAACAGGTTGTTTGTTT
ATTGAACAAACGAAAGTACACGTGATTTCAGAGCACATTTCCTACCCACA
GACTTGAATACTGGCAGTCTGGACGTTCGCGACATGGGTGTCGTTCCTTG
ATACATCGACTGCACGAACGAAACAACGTGGCGACCAGTGGTTGTAATGC
TACCGATGCACGTTCTTATCCATTCGAGACGGGTTTGATCCGATATTGTG
TTCATCGTGTTTCGTTTTCCCCCGTGCCTTTCATATTCCAACACAATAGT
ACGGGGATACCTGCTGGTGCCCTACTTCTCCCGACATCAACGACTACATC
ATAGACGAGGATGGTGACTGCGACAAGCCCTGCACCGGAGACCCGAGCAT
TTTCTGCGGTGAGTCCAGTATGGAGGGCAAGCGACATATGAGGTTTTTTG
AAGGCGCGGGATGGTGCGAAGGCGGAGAAAGGGATACGAACAGAGCTGAG
AGATGCAGTGAGGAAAACACTAGCCACCCAGGCTATCCGCCAGCACGCCC
CCCACACCCTCTAAAACAAACGTTCGGGCACTTGCAGCATACCGCGAACC
CTTGAACCGCCGACCATTCAGCAGACATTGGTTAGGATACGGCACGCACA
CACGCTGCCCCTCCCAACCTTGTTGCGTTTTCACCCCTTGTTCCAGGCGG
GACCTACTCTATTAGCATGTTCGAGTACCAGGAAGTCGAGAGCTCCAGCA
CCACAACGGGTATGTACGAGCAATACGAGAGCCCCGGCAGCACAACAGTT
ATATACGAGGAATACAAGAGCTCCAGCAGCAAAACAGTTATATACGAGGA
GTACGAGGGCTCCAGTGGCACGGCCGAGTACGACGATGACGAGGGCTACG
GCAGCTCAATTGGTGCGTAAACGTGATTTTGACACCGGGTTTTATAGCAT
TCAGTATGTATGTGCCCCCTTGGAATATATCGTTTGACATGAAGTCCCCC
ACGACTCATATGATTTGTCGCTTTCGTTCTCCCCCCGCGTCTCGATTTCA
CTGTACTCGATCAACACCGTGCTATCGTTGTTCGCGTGATCGTTGTTGAC
CAGAATCTGTGTGCAGTAACGGCGTCCCCGGCATCGAGTCCAATGGTGTT
TGCTGCGAGACCAGGTGCGTCGAAACAGTCATGCGTAGCCCAGTCGCCAT
TCCGCAGTTAGTTGGGATGAAGTTACTGTCGCTGGCGTATTTTACTATGG
TACAGATGCGTATGACGATAGTTGGCCTCGCGTGCCGACCACGCTGGGAC
CCGAACTCTCTCCATCTTCCCTCATCTCTAGATCAAATCCGAATCTAGAT
GTCTCGTTCGACACGCCGCCACCATTCTTAAGTGGGGTCTTAACGCAAGT
CCAAGGTCAATGGCAAATATCATCTCTTGACAAGATGGAAGAAAATGTAG
CTCGCCGGGAAAAGCGTTATCCCCTCATCAAGGAATCACAAATGCATGTT
CAGTTCGTGTCGCGATAGCACACATTTAGGCAATGGTGCATCAGCAGCCG
AGGCAAACGATTCCCGGCAACTGTGTGGGGAGCGCCTGTGCTCCACGGCG
AGCGTACGTGTCGCTCAAAACCCTTCTCCCCCAACCCTCACCAACCCTTT
CGCATGCGGGGTTCGAGAACCTGCAAACGCTTACTAGTCTGCGAGAATAT
GTGGGTTTTCATAACTATACTTACAAATCTCGGTTGTTTGGGTGCATTTT
GTCTCAACGCAGTTGCGGACAGTGCGGCGGTGCCGGTTGTTCGTCGGTTG
GCAACGGTGCCGAATCCTGCTGCACGAGTACGATTCTGGAGTTCGGGGAG
GTTTGCGGCTCGGCCCCCTGCGTCATCTAA
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7053:2389..4918+

>mRNA_E_fasciculatus_S2_contig7053.15217.1 ID=mRNA_E_fasciculatus_S2_contig7053.15217.1|Name=mRNA_E_fasciculatus_S2_contig7053.15217.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=732bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7053:2389..4918+ (Ectocarpus fasciculatus EfasUO2)
CAGGGCGAAGGGTTGACGAATGGCCAGCAGGCCCGGAACCTCAAGAAAAT
GAAAACCAAGAAGGTGAAAGCCAAGGCATCCACCGATCACGAGTACCTGG
GCTGCTATCGCTACAACAAGGACATTGGTGGGCTCTTGAAGCACGGAAAA
TCGTTCTTTGACATGACGGACCAGGCCCTGTGCCGTGCCCACTGCGAAGA
GTACGATACAGCCTTCTATGCCATGGAGTACGGGGATACCTGCTGGTGCC
CTACTTCTCCCGACATCAACGACTACATCATAGACGAGGATGGTGACTGC
GACAAGCCCTGCACCGGAGACCCGAGCATTTTCTGCGGCGGGACCTACTC
TATTAGCATGTTCGAGTACCAGGAAGTCGAGAGCTCCAGCACCACAACGG
GTATGTACGAGCAATACGAGAGCCCCGGCAGCACAACAGTTATATACGAG
GAATACAAGAGCTCCAGCAGCAAAACAGTTATATACGAGGAGTACGAGGG
CTCCAGTGGCACGGCCGAGTACGACGATGACGAGGGCTACGGCAGCTCAA
TTGAATCTGTGTGCAGTAACGGCGTCCCCGGCATCGAGTCCAATGGTGTT
TGCTGCGAGACCAGTTGCGGACAGTGCGGCGGTGCCGGTTGTTCGTCGGT
TGGCAACGGTGCCGAATCCTGCTGCACGAGTACGATTCTGGAGTTCGGGG
AGGTTTGCGGCTCGGCCCCCTGCGTCATCTAA
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