prot_C-tenellus_contig10062.1.1 (polypeptide) Choristocarpus tenellus KU2346

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview


Name	mRNA_C-tenellus_contig10062.1.1
Unique Name	prot_C-tenellus_contig10062.1.1
Type	polypeptide
Organism	Choristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Sequence length	219

Homology

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A6H5L1P3_9PHAE (Valine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L1P3_9PHAE)

HSP 1 Score: 355 bits (912), Expect = 1.560e-117
Identity = 171/218 (78.44%), Postives = 190/218 (87.16%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQGREISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNWSPSLQTAVSDLEVEY+EE GKLYYFKYM+ G  G +GE+LSVATTRPETILGDTAVCVHPED R+KHLVGGKVVVP+ GREI IIAD+YV+ EFGTG LKITPGHDPNDYEIGKRHSL TIN+ME+D T+ K  G AY G DRF CR  +WADME  GL +K EP+ QRVPRSQRGGEVIEPLVSSQWFV+TEGMGA+AL AV+EK ++ILPER
Sbjct:  321 MVNWSPSLQTAVSDLEVEYSEEDGKLYYFKYMVAG--GKEGEHLSVATTRPETILGDTAVCVHPEDERFKHLVGGKVVVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRHSLQTINLMEKDGTMAKTAG-AYAGQDRFDCRNNMWADMEAAGLALKAEPYLQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVQEKDIQILPER 535

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: D7FV40_ECTSI (Valine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FV40_ECTSI)

HSP 1 Score: 354 bits (908), Expect = 1.830e-112
Identity = 170/218 (77.98%), Postives = 190/218 (87.16%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQGREISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNWSPSLQTAVSDLEVEY+EE GKLYYFKYM+ G  G +GE+LSVATTRPETILGDTAVCVHPED R+KHLVGGKVVVP+ GREI IIAD+YV+ EFGTG LKITPGHDPNDYEIGKRHSL TIN+ME+D T+ +  G AY G DRF CR  +WADME  GL +K EP+ QRVPRSQRGGEVIEPLVSSQWFV+TEGMGA+AL AV+EK ++ILPER
Sbjct:  294 MVNWSPSLQTAVSDLEVEYSEEDGKLYYFKYMVAG--GKEGEHLSVATTRPETILGDTAVCVHPEDERFKHLVGGKVVVPLSGREIPIIADDYVDMEFGTGALKITPGHDPNDYEIGKRHSLQTINLMEKDGTMAETAG-AYAGQDRFDCRNNMWADMEAAGLALKAEPYQQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALAAVQEKDIQILPER 508

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A7S2A8Z3_TRICV (Valine--tRNA ligase (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2A8Z3_TRICV)

HSP 1 Score: 335 bits (860), Expect = 2.300e-109
Identity = 158/219 (72.15%), Postives = 190/219 (86.76%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQG-REISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNW+P L+TAVSDLEVEY+EE+GKLYYFKYM+ G  GG+ +YL VATTRPETI+GDTAVCVHPED R+KHL+G  VVVP+ G R I IIADEYV+ EFGTG LKITPGHDPNDY +GK++ L  IN+M +DAT+    G AY+G+DRF CRE+LW+DMEKEGLVIK +PHTQRVPRSQRGGE+IEPLVSSQWFV+TEGMGA+AL+AV++  +KI+P+R
Sbjct:  106 MVNWAPLLKTAVSDLEVEYSEEEGKLYYFKYMVEG--GGEEDYLPVATTRPETIVGDTAVCVHPEDERFKHLIGKSVVVPMSGGRAIPIIADEYVDMEFGTGALKITPGHDPNDYALGKKYDLEIINVMNKDATMNGNAG-AYEGMDRFECREKLWSDMEKEGLVIKADPHTQRVPRSQRGGEIIEPLVSSQWFVKTEGMGAKALKAVEDGDIKIVPQR 321

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A7S3XNU9_HETAK (Valine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XNU9_HETAK)

HSP 1 Score: 345 bits (884), Expect = 2.520e-109
Identity = 164/219 (74.89%), Postives = 184/219 (84.02%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGE-HGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQGREISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNWSP LQTAVSDLEVEY+EE+GKLYYFKYML     GG  ++L VATTRPETILGDTAVCVHPEDPRYKHL+G  VVVP QGREI IIADEYV+ EFGTG LKITPGHDPNDYE+GK+H+LPTINIM RDA+I + GG  Y GLDR  CRERLWADM   GLV+K +PHTQRVPRSQRGGEVIEP+VS+QWF R  GM  ++LEAV+   +KI+PER
Sbjct:  252 MVNWSPHLQTAVSDLEVEYSEEEGKLYYFKYMLADPAEGGGADHLPVATTRPETILGDTAVCVHPEDPRYKHLIGKTVVVPTQGREIPIIADEYVDMEFGTGALKITPGHDPNDYELGKKHALPTINIMNRDASINEAGGP-YAGLDRIACRERLWADMGAAGLVLKADPHTQRVPRSQRGGEVIEPMVSTQWFCRMGGMAEKSLEAVRSGEIKIIPER 469

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A7S2KNQ8_9STRA (Valine--tRNA ligase (Fragment) n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2KNQ8_9STRA)

HSP 1 Score: 319 bits (818), Expect = 1.560e-107
Identity = 156/215 (72.56%), Postives = 180/215 (83.72%), Query Frame = 0

Query:    5 SPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQG-REISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            +P L+TAVSDLEVEY+EE+GKL+YFKYM+ G      E+L VATTRPETILGDTAVCVHPED RYKHLVG   VVP+ G R I IIAD+YV+ EFGTG LKITPGHDPNDY IGKR+ LP INIM +D ++ +  G+ Y GLDRF  RE+LWADME EGLVIKV+PHTQRVPRSQRGGEVIEPLVSSQWFV+TEGMGA+AL AVK+  +KI+P+R
Sbjct:    1 APLLKTAVSDLEVEYSEEEGKLFYFKYMVEGSE----EFLPVATTRPETILGDTAVCVHPEDERYKHLVGKNAVVPMSGGRSIPIIADDYVDMEFGTGALKITPGHDPNDYAIGKRYDLPIINIMNKDGSLNENAGD-YSGLDRFDVREKLWADMEGEGLVIKVDPHTQRVPRSQRGGEVIEPLVSSQWFVKTEGMGAKALNAVKDGDIKIVPKR 210

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A7S3PW07_9STRA (Valine--tRNA ligase (Fragment) n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3PW07_9STRA)

HSP 1 Score: 320 bits (821), Expect = 5.630e-107
Identity = 152/219 (69.41%), Postives = 184/219 (84.02%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQG-REISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNW+P L+TAVSDLEVEYTEE+GKLY+FKYM+        E+L VATTRPETI+GDTAVCV+PED R+KHLVG  VVVP+ G R I +I+D+YV+ EFGTG LKITPGHDPNDY IGKR+ LP INIM +D ++ +  G+ Y GLDRF  R++LW DMEKEGLVIKV+PHTQR+PRSQRGGE+IEPLVSSQWFV+TEGMGA+A +AV++  +KI+PER
Sbjct:    8 MVNWAPLLKTAVSDLEVEYTEEEGKLYFFKYMIEDSD----EFLPVATTRPETIMGDTAVCVNPEDDRFKHLVGKNVVVPMSGGRTIPVISDDYVDIEFGTGALKITPGHDPNDYAIGKRYDLPIINIMNKDGSLNENAGD-YNGLDRFDARDKLWEDMEKEGLVIKVDPHTQRIPRSQRGGEIIEPLVSSQWFVKTEGMGAKAYKAVEDGNIKIVPER 221

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A1E7EWR8_9STRA (Valine--tRNA ligase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EWR8_9STRA)

HSP 1 Score: 334 bits (857), Expect = 3.560e-106
Identity = 155/219 (70.78%), Postives = 185/219 (84.47%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPI-QGREISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNW+P L+TAVSDLEVEYTEE+GKLYYFKYM+ G  GGK E+L VATTRPETI+GDTA+CVHP+D RYK LVG + +VP+  GR I IIAD+YV+ EFGTG LKITPGHDPNDY IGK++ LP INIM +D ++    GE Y GLDRFV RE+LW DME E L IKV+PHTQRVPRSQRGGE+IEPLVSSQWFV+TEGMGA+AL+AV++  ++I+P+R
Sbjct:  133 MVNWAPLLKTAVSDLEVEYTEEEGKLYYFKYMVDGTDGGKFEFLPVATTRPETIMGDTAICVHPDDERYKDLVGKEAIVPMGNGRTIPIIADDYVDMEFGTGALKITPGHDPNDYAIGKKYDLPIINIMNKDGSLNGNAGEQYNGLDRFVAREQLWKDMEDESLTIKVDPHTQRVPRSQRGGEIIEPLVSSQWFVKTEGMGAKALKAVEDGDIEIVPKR 351

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A7S2AXJ4_9STRA (Valine--tRNA ligase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2AXJ4_9STRA)

HSP 1 Score: 330 bits (847), Expect = 7.700e-105
Identity = 159/218 (72.94%), Postives = 180/218 (82.57%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQGREISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNWSPSLQTAVSDLEVE++EEQG LYYFKYML G      +Y+ VATTRPETILGD+AVCVHPEDPRY+  +G  V+VP QGREI +IADEYV+REFGTG LKITP HD NDYEIGKRH L  INIM +D TI K GG+ Y GLDR+  RE+LWADM+ EGLVIK EPHTQRVPRSQRGGEVIEP+VS+QWFV T+ M AQA++AVK K + ILP R
Sbjct:  133 MVNWSPSLQTAVSDLEVEFSEEQGTLYYFKYMLAGSD----DYIPVATTRPETILGDSAVCVHPEDPRYQQFIGKTVLVPSQGREIPVIADEYVDREFGTGALKITPAHDQNDYEIGKRHDLEMINIMNKDGTINKAGGK-YTGLDRYDAREQLWADMDTEGLVIKAEPHTQRVPRSQRGGEVIEPMVSTQWFVDTKDMAAQAVDAVKNKEITILPPR 345

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: B8CEW1_THAPS (Valine--tRNA ligase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CEW1_THAPS)

HSP 1 Score: 330 bits (847), Expect = 1.080e-104
Identity = 159/226 (70.35%), Postives = 183/226 (80.97%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQG--------REISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNWSP L TAVSDLEVEYTEE+GKLYYFKYM+ G  GG  EYL VATTRPETI GDTAVCV+P D RYKHL+G + +VP+          R I II+DEYV+ EFGTG LKITPGHDPNDYE+GK+  L  +NIM +DAT+ KE GE Y+GLDRF  R+RLW+DME EGLVIKVEPHTQRVPRSQRGGE+IEPLVS QWFV+TEGMGA+AL AVK+  ++I+P R
Sbjct:  131 MVNWSPGLMTAVSDLEVEYTEEEGKLYYFKYMVEG--GGDDEYLPVATTRPETIFGDTAVCVNPNDDRYKHLIGKRALVPMSSLTSKDSKPRSIPIISDEYVDIEFGTGALKITPGHDPNDYELGKKFDLEIMNIMNKDATMNKECGEKYEGLDRFEARDRLWSDMEDEGLVIKVEPHTQRVPRSQRGGEIIEPLVSKQWFVKTEGMGAKALAAVKDGDIEIVPSR 354

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BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Match: A0A1Z5K4B9_FISSO (Valine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K4B9_FISSO)

HSP 1 Score: 332 bits (850), Expect = 3.330e-104
Identity = 158/219 (72.15%), Postives = 185/219 (84.47%), Query Frame = 0

Query:    1 MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTRPETILGDTAVCVHPEDPRYKHLVGGKVVVPIQG-REISIIADEYVEREFGTGVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVCRERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMGAQALEAVKEKRVKILPER 218
            MVNW+P L+TAVSDLEVEY+EEQGKLYYFKYM+ G      E+L VATTRPETI+GDTAVCVHPED RYKHLVG KVVVP+ G R I IIADEYV+ EFGTG LKITPGHDPNDY +GK+  LP IN+M RD ++    G+ Y+G DRF CRE LWADMEKEGLVIKV+PH QRVPRSQRGGE+IEPL+SSQWFV+TEGMGA+AL+AV++  ++I+P+R
Sbjct:  255 MVNWAPQLKTAVSDLEVEYSEEQGKLYYFKYMVDGSE----EFLPVATTRPETIVGDTAVCVHPEDERYKHLVGKKVVVPMSGGRTIPIIADEYVDMEFGTGALKITPGHDPNDYTLGKKFDLPIINVMNRDGSMNANAGK-YEGQDRFECRESLWADMEKEGLVIKVDPHNQRVPRSQRGGEIIEPLISSQWFVKTEGMGAKALKAVQDGDIQIVPQR 468

The following BLAST results are available for this feature:

BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot

BLAST of mRNA_C-tenellus_contig10062.1.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A6H5L1P3_9PHAE	1.560e-117	78.44	Valine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 13...	[more]
D7FV40_ECTSI	1.830e-112	77.98	Valine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 ...	[more]
A0A7S2A8Z3_TRICV	2.300e-109	72.15	Valine--tRNA ligase (Fragment) n=1 Tax=Trieres chi...	[more]
A0A7S3XNU9_HETAK	2.520e-109	74.89	Valine--tRNA ligase n=1 Tax=Heterosigma akashiwo T...	[more]
A0A7S2KNQ8_9STRA	1.560e-107	72.56	Valine--tRNA ligase (Fragment) n=1 Tax=Leptocylind...	[more]
A0A7S3PW07_9STRA	5.630e-107	69.41	Valine--tRNA ligase (Fragment) n=1 Tax=Chaetoceros...	[more]
A0A1E7EWR8_9STRA	3.560e-106	70.78	Valine--tRNA ligase n=1 Tax=Fragilariopsis cylindr...	[more]
A0A7S2AXJ4_9STRA	7.700e-105	72.94	Valine--tRNA ligase n=1 Tax=Dictyocha speculum Tax...	[more]
B8CEW1_THAPS	1.080e-104	70.35	Valine--tRNA ligase n=1 Tax=Thalassiosira pseudona...	[more]
A0A1Z5K4B9_FISSO	3.330e-104	72.15	Valine--tRNA ligase n=2 Tax=Fistulifera solaris Ta...	[more]

Pages

InterPro

Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002303	Valine-tRNA ligase	PRINTS	PR00986	TRNASYNTHVAL	coord: 179..192 score: 52.38 coord: 63..80 score: 56.79
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 1..217 e-value: 8.0E-60 score: 202.7
IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	GENE3D	3.90.740.10		coord: 22..171 e-value: 1.5E-47 score: 162.8
IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	SUPERFAMILY	50677	ValRS/IleRS/LeuRS editing domain	coord: 20..175
None	No IPR available	PANTHER	PTHR11946:SF93	VALYL-TRNA SYNTHETASE, ISOFORM C	coord: 1..218
None	No IPR available	PANTHER	PTHR11946	VALYL-TRNA SYNTHETASES	coord: 1..218
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 2..212

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
C-tenellus_contig10062	contig	C-tenellus_contig10062:90..4832 -

Analyses

This polypeptide is derived from or has results from the following analyses


Analysis Name	Date Performed
InterProScan on OGS1.0	2022-09-29
Diamond blastp: OGS1.0 vs UniRef90	2022-09-16
Choristocarpus tenellus KU2346 OGS1.0	2022-07-08

Relationships

This polypeptide derives from the following mRNA feature(s):


Feature Name	Unique Name	Species	Type	Position
mRNA_C-tenellus_contig10062.1.1	mRNA_C-tenellus_contig10062.1.1	Choristocarpus tenellus KU2346	mRNA	C-tenellus_contig10062 90..4859 -

Sequences

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>prot_C-tenellus_contig10062.1.1 ID=prot_C-tenellus_contig10062.1.1|Name=mRNA_C-tenellus_contig10062.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=219bp
MVNWSPSLQTAVSDLEVEYTEEQGKLYYFKYMLGGEHGGKGEYLSVATTR
PETILGDTAVCVHPEDPRYKHLVGGKVVVPIQGREISIIADEYVEREFGT
GVLKITPGHDPNDYEIGKRHSLPTINIMERDATIGKEGGEAYQGLDRFVC
RERLWADMEKEGLVIKVEPHTQRVPRSQRGGEVIEPLVSSQWFVRTEGMG
AQALEAVKEKRVKILPER*

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **INTERPRO**
Term	Definition
IPR002303	Valyl-tRNA_ligase
IPR002300	aa-tRNA-synth_Ia
IPR009008	Val/Leu/Ile-tRNA-synth_edit