mRNA_C-tenellus_contig7123.2.1 (mRNA) Choristocarpus tenellus KU2346

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: D7FQY5_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FQY5_ECTSI)

HSP 1 Score: 312 bits (799), Expect = 4.950e-94
Identity = 191/494 (38.66%), Postives = 278/494 (56.28%), Query Frame = 1

Query:    1 GGDLSFSRIPKLLSGCLHTCCQSCMEESLQRYRGVAEAPCPICGIIT-ETADTNDLVNNYVVLRGV-RARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLK--EVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHE--FTLLPQAEEKHR-GLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFSCDKGILDCARHQISKLGSV-NSISAVKEAGSKVQEQEQVEGTK------LHFNLEASVGVHPRHVGFAVPTDKVVVEVRGGSEAEVLSGGKEGPLIGRVILVTQLEGAGQTSFQSEQLFESLSQASNMGSSS---------------SASNVSTSIPTVRFTKVLRV 1395
            GGDL+FS IPK+L GCLHT CQ+C EE LQR +  +   CP+CG+ T +   T+ L +N V L+ V R    CD CDD   A H C +C   LC FH+  H  SRGTKSH+V+ LK  E   R         + + C +H+   AKLFCS PC   ICH+CS+  H GH   F+   + E+ HR GL R+ V+    RLA ++  L +++  +  V+ + +  E   ++ FS +R A+D REK ++K L+     K + L+ Q+  L +V+ AY+ ALD+    L++LH D+F+A  P I + +DA + +  SL+L PCV+D + F  D+ I +  R  IS+LG V N++   K+       ++++  +       +HF+L A VG  P+    A PTDKV+VE+R GSE + L  G  GP+IGRV L T+ +GA   ++++E  FESLSQ +     S               + S     +P VRFTKVLRV
Sbjct:   27 GGDLTFSHIPKVLKGCLHTICQNCAEEGLQRSKDGSSIMCPLCGVSTSDVTSTSALQDNIVALQDVHRKNSHCDFCDDVAKASHVCHECASLLCTFHMTAHSVSRGTKSHRVVSLKNREASHR---------VQITCARHRDVEAKLFCSEPCGTTICHNCSVAEHSGHSVFFSDSAEVEQVHRAGLTRRGVSLGH-RLARVRGGLDAVEAVKTGVEKHTRLAESATVQAFSAIRAAVDSREKEILKNLRADRAQKTQDLNAQLAKLGQVMRAYEIALDSTNTSLERLHGDQFLAMEPTIRECLDAREAEFPSLELTPCVEDRVEFEHDRSIANALRQGISRLGCVVNAVRPRKKKEKSTAPEQELHASSTPSEHNIHFSLAAIVGARPQEATLASPTDKVIVEIRSGSEHDFLKSGTTGPVIGRVALHTKFKGAKHLAYETEHFFESLSQRATPTRQSRKQQHQAEKERQKQPTPSRDGDCVPAVRFTKVLRV 510          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: UPI00109B775A (uncharacterized protein LOC110244904 n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI00109B775A)

HSP 1 Score: 116 bits (291), Expect = 4.110e-23
Identity = 79/277 (28.52%), Postives = 138/277 (49.82%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAPCPICGIITETADTNDL-----VNNYVVLRGV---------RARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALD 816
            P++L  CLHT C+ C+   L++        CPIC +I++  D + L     VNN + L  +           + LC +C+    A  +C DCG  +C   +K H+     K+H+++ L+E+  +GI S+      + C +H+ E+ KLFC + C  LIC DC+++ HKGH+F   P   +  +G L+K      E    I+ +L+++ E +D V+ N +  ++ + RT       ++   + L +    +   K++ LS Q   LE  +AA    L+
Sbjct:   26 PRILP-CLHTYCRKCLFNLLEK----GSIQCPICRVISQVKDVSSLQVNIWVNNRLSLLELSKVEKXSSEEEQLLCGNCNLNQQATGKCFDCGTFMCDTCIKAHKLMAVLKNHQLLTLEEIKSKGIPSI---EKRILCGKHKDEQQKLFCEA-CETLICRDCTLIDHKGHQFGFXPDVIKGQKGNLQKKXQDLCETKKKIESALKAVXEMQDRVRKNEETLKKEIDRTIDAQIAVLNQIRRNLKQKSANLTATKMKRLSSQQDGLEMHLAALTNTLN 293          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: UPI001F03602A (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase TRIM71-like n=1 Tax=Xenia sp. Carnegie-2017 TaxID=2897299 RepID=UPI001F03602A)

HSP 1 Score: 112 bits (280), Expect = 7.120e-22
Identity = 89/348 (25.57%), Postives = 166/348 (47.70%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAPCPIC--------GIITETADTNDLVNN---YVVLRGVRARCL-CDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFSCDKGILDCARHQISKLGSVNSISAV 1035
            PK+L  CLH+ CQ C+++  +   G A   CP C        G + E   TN  +NN   ++ ++   ++ + C +C D + A  RC DC   LC   L  H+R++ TK H+V+ LK++    +Q  +  H PLYC QH  E  K +C + C   +C +C I+ H+ H++  L     K R  +R  V  +  ++  +++++ ++    + V +  Q  ++ + + FS   + +  +E+ L+  +     +K + L  Q + LE ++++  ++   +   L+  +  E M     + +R+  LQ     ++ EP  DD I +       D  R  I   G + + S V
Sbjct:   26 PKILP-CLHSFCQKCLKKISKD--GEALILCPTCRNEINLPPGGVQELP-TNFFINNMLDFISIQSFASKPIDCTNCQDGIHAVARCGDCVEFLCEQCLAAHKRTKLTKDHEVLALKDMQTASVQEKM--HRPLYCSQHDREILKYYCKT-CDDPVCRECLIMEHREHDYGYLKDVSMKQRQQVRLLVENTNSQIGSLEKAISNVTRLIERVNAKRQGIQQEITKNFSKCVEVLKEKEEELLVFVDNECRIKCKNLGLQKENLETLLSSISSSCQFSENVLEHGNEAEVMLVKKQLTKRLGELQNT--HMEFEPLEDDSIEYEF---FTDEFRRAIQHAGHIRAFSTV 361          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: UPI001F032E23 (E3 ubiquitin-protein ligase TRIM33-like isoform X1 n=2 Tax=Xenia sp. Carnegie-2017 TaxID=2897299 RepID=UPI001F032E23)

HSP 1 Score: 107 bits (268), Expect = 2.420e-20
Identity = 79/274 (28.83%), Postives = 136/274 (49.64%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGV--------AEAPCPICG--IITETADTNDLVNNYVVLRGVR--------ARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVA 795
            PKLL  CLH+ CQSC+E+ L+             A   CP CG   +        L++N  +L  V+        A   C SC+D   A   C++C   LC+  ++ H+R R TK H++   +E+ E  +++   S   LYC +H  E+ +LFC   C +L C DC +  HK H++  + +A  ++R   +K +   +E+LA + ES+ S+K +   ++   +     +  T +++  A+  RE  L+  L+ + + K + L +Q K L  + A
Sbjct:   39 PKLLP-CLHSFCQSCLEDRLRGNEPSTTSAVPCSARLKCPKCGQEFLVPARGVATLIDNQFILDIVKKPSRENESASHKCTSCEDDSDASSFCLNCSEWLCSACVQAHQRVRVTKDHEIKSGEELKE-SLRNNNRSERCLYCKKHPSEKLQLFCVK-CERLTCRDCQLHEHKHHQYQFVNEAAARYREFFKKKLICLEEKLAPLAESIDSVKNSTTHLELKSEAVVNDIKNTSNDIISAVRERESVLLNELQALVQFKRKLLLKQTKDLNLMQA 309          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: A0A8J1USH7_OWEFU (Ofus.G18670 protein n=1 Tax=Owenia fusiformis TaxID=6347 RepID=A0A8J1USH7_OWEFU)

HSP 1 Score: 105 bits (263), Expect = 8.310e-20
Identity = 105/365 (28.77%), Postives = 169/365 (46.30%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAPCPIC---GIIT----ETADTNDLVNNYV-------------VLRGVRARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLP-----SHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILS-HKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIK-------ALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFSCDKGILDCARHQISKLGSVNS 1023
            PK+L  CLH+ C+ C+E+   R  GV +  CP C    I+T    E   +N  +NN +             ++   R +  C SCD+   A   C DC   LC   ++ H+R R TK H + ++     +  Q + P        P YC  H+HE  +L+C S C K IC +C +L  H  H F  L  A E  + +  K +A ++  +  I+ES+       + V+   Q     V  T     QA++ RE+ L++ +++I +VKV++L  QI+       +L+  V   +T+L++ G  +D L   + M        +M  +++    LQ  P  DD+I F+     L  A   I +LG V S
Sbjct:   24 PKVLP-CLHSFCRRCLEDIFNRD-GVLQ--CPTCKQDAILTGSKIEGLPSNFFINNILDVVASHEEDLDSDIIGIKRPQPTCTSCDEGSNATSLCKDCNEYLCDNCVRAHQRVRLTKDHYITRIPSNSNQSTQMMSPIMPVSDRPPSYCELHEHEILRLYCDS-CTKAICRECMMLEEHSCHNFIYLQDAVENSKTVAVKLLADAKAGVRAIEESIMLANAMSERVEVRTQAVATEVRATTRRHMQALEERERELLRRVEKIRQVKVKSLHIQIEDLKEGFQSLQMTVNKVETSLES-GTDIDVLKTRDIMVA------QMQGMRKLRGHLQ--PHEDDNILFTPPDTALHAA---ICQLGYVAS 371          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: A0A7D9EPF6_PARCT (E3 ubiquitin- ligase TRIM71-like n=4 Tax=Alcyonacea TaxID=40677 RepID=A0A7D9EPF6_PARCT)

HSP 1 Score: 103 bits (258), Expect = 3.500e-19
Identity = 82/322 (25.47%), Postives = 154/322 (47.83%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAPCPIC--------GIITETADTNDLVNN---YVVLRGVRARCL-CDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISF 957
            PK+L  CLH+ CQ C++  L+   G     CP C        G + E   TN  +NN   ++ ++   ++ + C +C D   A  RC DC   LC   L  H+R++ TK H+V+ LK++    +Q  +  H PLYC QH  E  K +C + C   +C +C I+ H+ H +  L     K R  ++  V  ++ +   ++++++ +    + +    +  ++ +   FS   + +  +E+ L+  +     +K + L  Q ++LE ++A+  ++   +   L+  +  E M     + QR++ LQ     +  EP  DD I +
Sbjct:   26 PKILP-CLHSFCQKCLQRILKE--GENAILCPTCRNEIQLPQGGVQELP-TNFFINNMLDFITVQSFSSKPIDCTNCQDHSHAVARCGDCVEFLCEQCLAAHKRTKLTKEHEVMALKDLQSSDVQDKM--HRPLYCSQHDREILKYYCET-CDDPVCRECLIMEHREHAYGYLKDVNNKQRQEVKSLVDSTKAQTVPLEKAIEDITTLMERLGVRSKGLQQEITSNFSKCTEVLKEKEEELLVFVSNECRIKCKNLGLQKESLETLLASVTSSCQFSENVLEHGNEAEVMLVKKQLTQRLNDLQNT--HMAFEPQEDDVIEY 338          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: A0A6J0H826_9PASS (tripartite motif-containing protein 45 n=24 Tax=Passeriformes TaxID=9126 RepID=A0A6J0H826_9PASS)

HSP 1 Score: 102 bits (254), Expect = 4.820e-19
Identity = 85/330 (25.76%), Postives = 142/330 (43.03%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEE--SLQRYRGVAEAP--CPICG--IITETADTNDLVNNYVVLRGVRARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFSCDKGILDCARHQI 999
            P+LL  CLH+ C  C+    +L+   G A  P  CP+C   +         L  + +      A   CD C D   A  RC+ CG+ LC F  + HRR + T SH V++L+   +        +  PL CP H  E  +LFC   C + +C +C +  H+ H +        +H   LRK +  +Q+ +  +++ L  + +   +V+S  +     +    S   +AI+     L+K L+ ++  K   L  Q   L++++    T ++     L      E + T   +  R+  L   A S    P VDD I FS  +    C  + +
Sbjct:   16 PRLLP-CLHSLCDPCLRRLGALEGEPGGAARPVLCPVCDAEVSLPPGGVGQLTPDCLAGSRAGAAAGCDLCADGAAA-GRCLTCGVRLCPFCCRAHRRQKKTASHAVMELENTKD-----CSQAGKPLLCPSHPTEELRLFCEQ-CDQPVCQECVLGRHRQHPYDFTSNVIHRHGDALRKLLKNTQQHMDTLEDVLSQIDKMGSAVRSRAEAVATEICLFASGYVRAIEEHRDRLLKQLEDLKVQKENLLHLQKAQLQQLLLDMRTGVEFTEHLLMSGSDLEILITKGVVSSRLTKLNSVAYSTH--PSVDDRIQFSAQERAGQCCGYAV 335          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: UPI000C6D29D8 (E3 ubiquitin-protein ligase TRIM71-like n=1 Tax=Centruroides sculpturatus TaxID=218467 RepID=UPI000C6D29D8)

HSP 1 Score: 102 bits (253), Expect = 1.350e-18
Identity = 87/340 (25.59%), Postives = 151/340 (44.41%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAP-----CPICG--IITETADTNDL--------VNNYVVL------RGVRARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQS--------VLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFS 960
            PK+L  C HT C  C E+    Y G A++      CP C   ++    D   L        +N+ + +       G     +C SCDD   A  RCIDC  ALC   L  H+R R TK H + ++ + G +  ++         +    P+ C  H+     L+C   C + +C +C++ SH+GH  + L +     + L+       +    VI ES++  +   + +    Q   + +  T      A++ RE+ L++ +++I +VKV  +  QI  L+      +  +D     LD L   +F+        ++  ++QQ  +L L+PC DD+I F+
Sbjct:   26 PKVLP-CYHTICGRCYEQ----YAGSADSMTGSYRCPKCNESVLQTLGDEQSLDGLHPPFYINDILDVVKEDFENGYSNNNMCTSCDDGNTASARCIDCKEALCENCLMAHQRVRLTKDHNITRVDDTGIKYAETKYSPPSLPAINERKPVPCEFHKTSIICLYCEQ-CNEALCRECTMRSHRGHTVSYLEEILNACKKLISGKCKDLKMESHVIDESIEMAQRMSERIDLKIQAVSKEIRATIRRHMNALEERERELLRRVEKIRQVKVHTIQLQIDELKVYKTKMNNLIDYTEQSLDNLVDVDFIKLRDNFVSQVGEIKQQHHNL-LKPCEDDNILFT 358          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: H0ZT12_TAEGU (Uncharacterized protein n=19 Tax=Passeriformes TaxID=9126 RepID=H0ZT12_TAEGU)

HSP 1 Score: 99.8 bits (247), Expect = 3.520e-18
Identity = 88/334 (26.35%), Postives = 140/334 (41.92%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCMEESLQRYRGVAEAP--------CPICG--IITETADTNDLVNNYVVLRGVRARCLCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEVGERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALDTAGLCLDQLHCDEFMATGPCIEQRMDALQQQAQSLQLEPCVDDHISFSCDKGILDCARHQI 999
            P+LL  CLH+ C+ C+     R  G  E P        CP+C   +         L  + +     RA   CD C D   A  RC+ CGL LC F  + HRR + T SH V +L+   +        +  PL CP H  E  +LFC   C + +C DC +  H+ H          +H   LR+ +  SQ+ +  +++ L  + E   +V+S  +     +        +AI+     L+K L+ ++  K   L  Q   L++++    T ++     L      E + T   +  R+  L   A S    P VDD I FS  +    C  +++
Sbjct:   16 PRLLP-CLHSLCEPCL-----RRLGPPEGPPGAARPVLCPVCDAELSLPAGGVGQLPPDCLAGSRGRAAAGCDLCADGAAA-GRCLTCGLRLCRFCCRAHRRQKKTASHAVTELENTKD-----CSQAGKPLLCPSHPTEELQLFCEQ-CDQPVCRDCVLGRHRQHPCDFTGNVIHRHGDALRELLKNSQQHMDTLEDVLSQVDEMGSAVRSRAEAVATEICLFARGYVKAIEEHRDRLLKQLEDLKVQKENLLHLQKAQLQQLLLDMRTGVEFTEHLLMSGSDVEILITKGVVSSRLTKLNSVAYSTH--PSVDDRIQFSPHERAGQCCGYEV 334          

BLAST of mRNA_C-tenellus_contig7123.2.1 vs. uniprot
Match: A0A401NGH9_SCYTO (Uncharacterized protein n=2 Tax=Scyliorhinus TaxID=7829 RepID=A0A401NGH9_SCYTO)

HSP 1 Score: 99.4 bits (246), Expect = 5.970e-18
Identity = 79/295 (26.78%), Postives = 135/295 (45.76%), Query Frame = 1

Query:   28 PKLLSGCLHTCCQSCM----EESLQRYRGVAEAP---------------CPICG--IITETADTNDLVNNYVVLRGV------RARC--LCDSCDDAVMACHRCIDCGLALCAFHLKDHRRSRGTKSHKVIKLKEV---GERGIQSVLPSHMPLYCPQHQHERAKLFCSSPCAKLICHDCSILSHKGHEFTLLPQAEEKHRGLLRKHVARSQERLAVIQESLQSMKETEDSVKSNCQNTERMVLRTFSNLRQAIDLREKTLVKALKRIEEVKVRALSRQIKALEKVVAAYDTALD 816
            PK+L  CLHT C  C+    + S+ R RG  E+                CP+C   +    A    L +N+++L  +      R+ C  +CD C+D   A  RC  C   LC F  K HRR + T SH  + L+++    ER +Q       P+ C  H  E  ++FC + C +L+C DC ++ H+GH    +     KH   +R+ + ++Q  +  +QE+LQ +      V+       + +        +A++     L+K L+ +   +  +L+ Q   LE+++A   T +D
Sbjct:   44 PKILP-CLHTFCLDCVRGLQQFSVPRTRGEGESGSGGSLDCTESAACILCPLCDYEVHVPAAGLRGLTSNHLILNDILLESLKRSDCRIVCDLCNDGD-AERRCHICSTNLCDFCSKAHRRQKMTASHNTVALRDLQGGNERLVQ-------PIICSAHPAEELRMFCET-CDRLVCRDCCMVDHRGHNCDFIASVISKHGIFIRELLKQTQPHIGALQETLQKIAHARQLVQERLGMMTKEINNFTDGYIRALENHRNQLLKELEELRAQRENSLNLQQIQLEQILADMRTGVD 328