mRNA_C-linearis_contig102.778.1 (mRNA) Chordaria linearis ClinC8C monoicous

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: D7FH28_ECTSI (JmjC domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FH28_ECTSI)

HSP 1 Score: 673 bits (1736), Expect = 6.620e-229
Identity = 363/529 (68.62%), Postives = 402/529 (75.99%), Query Frame = 2

Query:   95 MAQDPNTAGKWPGWAGLVVASAAVMCGLFIGGPPTLFSADKT----SILTIFAREAFDWSSSTTPAPLSATAVLVKAVLAGKSSIA-ELGLENAVLSASASAEGKTSIVDVRVGPVRDLISARILSKVKQLCDSSGTSSSCDPNEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAADGRLPS-PGSASTSAADL-----QDMRCEIPEVTVPLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAAD----REDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGP-AGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTLASVLGMGCEQ 1633
            MA+   T  +W GW  L+VASAAV+ G+ +GG   L    K+    SIL IFA+EAFDWSSSTTPAPLSATAV VKA+L GKS  A  LGLENAVLSA A  +G T  VDV+                                 VDPEFGTTPLHLAELWGS+ELVEYL+SIGASP+ FDTVGRQPRNMTFKAFS NSKKAAD RLP+ P SAS +A D       +MRCEIPEVT+PLFP A  +  A       QWQ S+L ALAEVRRLVSEGEPVMVRN+VPWLQ+Q+ +QL+FPDA++FAEAWGHR VDVGGVPYAK FDL NERTTL+DY+ ST   AAA A       +  A   +PNYVFQVDTEAC EGR+L  R+V+AALPSSGD+PIVCPPPGGLRGLESVHYYLGG+ SGAPFHIHSDALNLAVSG+K+WWVVTPR AVWSRRHIR+YAEE KGGP A GEDRPMECVQ GGDLVYVPGDWGHAAMNLE GTFGYTLELVN RDTLASVLGMGCEQ
Sbjct:    1 MARATTTDRRWSGWVSLLVASAAVITGVLLGGTHLLLPDKKSKTSPSILMIFAKEAFDWSSSTTPAPLSATAVAVKAILGGKSDGALALGLENAVLSA-AGPDGSTRAVDVK---------------------------------VDPEFGTTPLHLAELWGSSELVEYLLSIGASPDAFDTVGRQPRNMTFKAFSANSKKAADARLPALPDSASAAAGDRGMPQDDEMRCEIPEVTIPLFPTADGDD-AAELHDHDQWQSSALAALAEVRRLVSEGEPVMVRNVVPWLQQQEKKQLQFPDAASFAEAWGHRPVDVGGVPYAKNFDLLNERTTLSDYMKSTKTTAAAAADSSGRGNDQKATGPAPNYVFQVDTEACAEGRDLLGRVVDAALPSSGDRPIVCPPPGGLRGLESVHYYLGGRHSGAPFHIHSDALNLAVSGRKRWWVVTPREAVWSRRHIRDYAEEGKGGPPASGEDRPMECVQRGGDLVYVPGDWGHAAMNLEDGTFGYTLELVNRRDTLASVLGMGCEQ 494          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A835YWU2_9STRA (JmjC domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YWU2_9STRA)

HSP 1 Score: 285 bits (730), Expect = 2.070e-80
Identity = 197/528 (37.31%), Postives = 267/528 (50.57%), Query Frame = 2

Query:  149 VASAAVMCGLFIGGPPTLFSADKTSILTIFAREAFDWSSSTTPAPLSATAVLVKAVLAG--KSSIAELGLENAVLSASASAEGKTSIVDVRVGPVRDLISARILSKVKQLCDSSGTSSS-----CDPNEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAADGRLPSPGSASTSAADLQDMRCEIPEVTV-PLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDY-----------IDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGR--------ELFERIVEAALPSSGDK-----PIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEE------------------------------SKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMN 1546
            + S+ V+ G+F     T     +  IL   A+   +W  STT AP+S  +VL K +LA   K     L L+   L+A  +A+G    V +     R  I AR+L +V  LC S+  + +     CDPNE DP FG TPLH+A L G   L EYL++IGA P + DTVGR+P NMTF++F+ NSKK A  R    GS            C+IPEV++ P  P A                      L EVRRLV EGEPVMVRN + WL   + E L +P ++ FAEAWG R VDV GVPYA  F++   R+TL+ +             ST       AA  +   A+ +    ++     C+           EL    V AALP  G       P++CPP  G  G++ +HY++G + +GAPFH+HSDA+NL ++G KKWWV+ PR A +SR HI+E+A +                              ++ G     D PMEC+Q+ GDL YVP DWGHAA+N
Sbjct:   43 IISSVVIKGIFDKNHHT-----ELPILHTIAKHNMNWVDSTTAAPISVPSVLAKLMLAKEPKDWPEGLTLDEETLAAGTNAKGWRHTVPLSAA--RRSIEARVLQEVAALCGSTTRARTEAGPLCDPNERDPVFGMTPLHMARLQGDDALAEYLLTIGADPNVEDTVGRKPSNMTFQSFAVNSKKWALAR----GS-----------ECQIPEVSIDPANPEA---------------------GLREVRRLVGEGEPVMVRNAMAWLSAARGEPLLYPTSAKFAEAWGERPVDVAGVPYAAKFNVTTARSTLSAFRAEHMGGSDALATSTGDTPLLKAAKSDKKPAKNAEKKRWKFTLATCLPLNDDTQLLCCELMRDYVMAALPVQGGLETPGGPLMCPPTNG--GMDIMHYFMGDKGTGAPFHVHSDAINLMLTGSKKWWVLPPRQATFSRMHIKEWAAKLCTASGDALHALSADLDSTLHLLFARAPLGTQTGRLESRDAPMECLQNAGDLAYVPFDWGHAAIN 525          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A2V3JAZ6_9FLOR (JmjC domain-containing protein 8 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3JAZ6_9FLOR)

HSP 1 Score: 244 bits (623), Expect = 1.260e-67
Identity = 163/473 (34.46%), Postives = 235/473 (49.68%), Query Frame = 2

Query:  191 PPTLFSADKTSILTIFAREAFDWSSSTTPAPLSATAVLVKAVLAGKSSIAELGLENAVLSASASAEGKTSIVDVRVGPVRDLISARILSKVKQLCDSSGTSSSCDPNEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAADGRLPSPGSASTSAADLQDMRCEIPEVTVPLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGE-DRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTLA 1606
            PPTL       +L+  AR  F W+SSTTP P+S+ +V  K++L+   +     L   + SA            + + PVRD  +A  L+K+++LC     +  C  +  D  +G TPLHLA + G + L E+L+  GA   + D  GR+P N++F  F  N+K  A  + P                C+ P V                      ++     A +EVRRLV+EGEP+++R    +       Q R+P  S     + H  V VG VPYA  F+L   R  L DY  +  + ++             +P+YVF    E C    +    +V    P S     +   P    GL+ +H++LG + SGAPFH+H+DALN AVSG K+W+V TP   ++SRR I+ + E     PA  E D+P+EC+Q  GD+VYVP DWGHA +NLE  TFG  LE++N RDTLA
Sbjct:   29 PPTL------DVLSFAARHQFAWASSTTPVPISSVSVFAKSLLSRDKAALPPPLVRLISSADT----------IPLQPVRDYTNALRLAKLRRLC----ATLPCVYDRQDDVYGLTPLHLAAISGDSALSEWLVKHGADA-VEDFAGRKPSNLSFANFIRNAKSVAQKQHP---------------ECDFPTV---------------------HFEHDVEHAKSEVRRLVNEGEPILMRAAYDYYN-----QHRYPPVSQLVREYAHVNVTVGSVPYANAFNLSTTRMKLEDYYRTIYQESST------------APSYVFNKHPEICQTAYQALSALVADTFPLS-----LISHPDNTGGLDGIHFFLGNKHSGAPFHVHADALNAAVSGSKQWYVYTPARTIYSRRPIKTWVENDL--PALEEHDKPLECLQRAGDVVYVPLDWGHAVLNLEHDTFGVALEVLNRRDTLA 420          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A0H5R404_9EUKA (JmjC domain-containing protein n=1 Tax=Spongospora subterranea TaxID=70186 RepID=A0A0H5R404_9EUKA)

HSP 1 Score: 229 bits (583), Expect = 2.180e-62
Identity = 157/486 (32.30%), Postives = 240/486 (49.38%), Query Frame = 2

Query:  146 VVASAAVMCGLFIGGPPTLFSA-DKTSILTIFAREAFDWSSSTTPAPLSATAVLVKAVLAGKSSIAELGLENAVLSASASAEGKTSIVDVRVGPVRDLISARILSKVKQLCDSSGTSSSCDPNEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAADGRLPSPGSASTSAADLQDMRCEIPEVTVPLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDT 1600
            ++A A ++C + +G   ++    +   ++ ++A    +WS STT  P+S ++VL K +L+   +    GL + +   S +         V+V  +R  I+     K+++LC S+G   SCD    DP +  TP+H+A L G    ++YL+S+GA+P+  D   R+P N+TF +F  N+++ A  R    GSA           C++PEV +                      E+ L+A AE+RRLV EGEPV +R +   LQ   +  L   +   F + +G+  V VG VPY+  F L + +  L++Y+ +                      YVF  D E   +     +R V    P     P V        G ES+H+YLG + SGAP+H HSDA+NL   GKK+W +  P  + +SR  I +Y E S   PA      + C Q+ GD  YVP DWGHA +NLE  TFG+ LEL+N RD+
Sbjct:    6 IIAFAILICAVLVGFRTSMVRPMNSIEVMRVWADH--NWSMSTTSLPISRSSVLAKNILSKNFN----GLPSYLHVQSDT---------VQVSLIRRYINQDRYKKLRKLCPSAG---SCDVITPDPVYKLTPMHMASLSGDQNSIDYLLSLGANPDAIDASARKPANLTFSSFIENTRRFAKQR----GSA-----------CQLPEVIID--------------------PENPLIAFAEIRRLVDEGEPVAIRGL---LQYMNESALLDWNLDIFLKQFGNVQVKVGSVPYSSYFGLESAKMALHEYVSNA-----------------TDNTYVFNKDPEISADAMSFLDRFVTETFPKYFTAPRVL-------GDESIHFYLGQKGSGAPYHQHSDAVNLLTHGKKRWMITPPNDSAYSRVPIHDYVESSGQIPA------LTCEQYAGDAFYVPFDWGHAVLNLEDYTFGFALELLNDRDS 405          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A2P6NVY1_9EUKA (JmjC domain-containing protein n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NVY1_9EUKA)

HSP 1 Score: 209 bits (532), Expect = 4.230e-55
Identity = 129/370 (34.86%), Postives = 189/370 (51.08%), Query Frame = 2

Query:  509 NEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAAD--GRLPSPGSASTSAADLQDMRCEIPEVTVPLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTLASV 1612
            ++ DPEFG T LHLA   G  E +++L S+GA  ++ D+VGR P N++F  F  NSKK A   GR                  C+IP V V                        S    +E++RLV EGEPV+++N + +L    +  L         E +G   V VG VPYA +F L ++ +TL DY     +  +              P Y+FQ  ++    G      +V  A P+     ++CP   G  G +S+H+YLG + SGAPFHIH+DA NL V G K+W +  P  A++S++H+  + +E        ED+P+EC+Q  GD++YVP DW HA +N E GT+GY LEL+N R+ + S+
Sbjct:  134 DQQDPEFGLTALHLAHQSGDAEAIKFLESLGAKLDVQDSVGRLPSNLSFTNFIKNSKKWARQAGRT-----------------CDIPVVDV-----------------------KSEADFSEIKRLVGEGEPVLMKNAMKFLVNANE--LAAIQIQDLVEKYGSSHVTVGEVPYANVFRLPHDISTLRDYYVHHVQNKSE------------HPLYIFQKHSQMTEAGLTALGDLVSRAFPTPS---LICPVEYGSTGSDSIHFYLGAKNSGAPFHIHADAANLVVRGSKRWLMYPPLQALYSQKHVYRWLQEDYKDMRE-EDKPLECIQGPGDVIYVPFDWSHAVLNEEDGTYGYALELLNRREVMWSL 445          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A0G4J4W2_PLABS (JmjC domain-containing protein n=1 Tax=Plasmodiophora brassicae TaxID=37360 RepID=A0A0G4J4W2_PLABS)

HSP 1 Score: 206 bits (524), Expect = 3.060e-54
Identity = 136/398 (34.17%), Postives = 203/398 (51.01%), Query Frame = 2

Query:  410 VRVGPVRDLISARILSKVKQLCDSSGTSSSCDPNEVDPEFGTTPLHLAELWGSTELVEYLISIGASPEIFDTVGRQPRNMTFKAFSTNSKKAADGRLPSPGSASTSAADLQDMRCEIPEVTVPLFPAATANKPAVSEQQRQQWQESSLVALAEVRRLVSEGEPVMVRNMVPWLQKQQDEQLRFPDASAFAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTL 1603
            V V  +R  ++A    +++ +C ++G    CD    DP+ G TPLH+A +      + YL+++GA P+  D  GRQ RN++F  F  N+++AA+ R    GS            C++PEV +     A  ++          W        AE+RRL  EGEPV +R ++   ++   + L + D   F    GH  V+VG VPYA+ F L  +   L+ Y+ S A  +A+               YVF  D   C +  ++ +R    ALP     P    P     G ++VH+YLG + SGAPFH+HSDA+NL   G K W+V  P  +V+SR  I E+A     G +G E   + C Q+ GD +Y+P DWGHA +N E  TFG+ +EL+N RD+L
Sbjct:   88 VPVALLRAHLNAGRHMRLRDMCPTAGA---CDLQGADPDHGLTPLHMAAMRDDRSSIAYLMALGADPDAIDRAGRQYRNLSFTNFVRNARRAAEER----GST-----------CQLPEVNLAGLGRADLDR---------SW--------AEIRRLAHEGEPVAIRGLLGAYER--SDVLDW-DLDTFLTRHGHVPVNVGDVPYAQYFGLPIQSMPLSTYVASLAPGSAS---------------YVFAKDDGICRDALQILDRFARDALP-----PYFVSP--AALGSDAVHFYLGNKGSGAPFHLHSDAVNLLAHGSKTWFVTPPPQSVYSRTPIGEFAAN---GTSGIES--LRCEQNPGDAIYIPFDWGHAVLNNEDSTFGFAVELLNKRDSL 420          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A7S0G3X7_9RHOD (Hypothetical protein n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S0G3X7_9RHOD)

HSP 1 Score: 113 bits (283), Expect = 1.020e-25
Identity = 51/110 (46.36%), Postives = 76/110 (69.09%), Query Frame = 2

Query: 1274 GLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTL 1603
            G  ++H+ LG + SGAP HIHSDALNL + GKK+W+V+ P+ A++SR+HI  + +E    P   + +P+ECVQ+ GD +YVP DW H  +N    TF + LEL++ R++L
Sbjct:   16 GPSALHFALGREGSGAPMHIHSDALNLVLGGKKRWFVLPPQDAIYSRKHISTWVKEDL--PTA-DPKPLECVQNSGDAIYVPFDWAHGVINSGEFTFAFALELLSDRESL 122          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A7S3FJ58_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3FJ58_9EUKA)

HSP 1 Score: 91.7 bits (226), Expect = 4.140e-18
Identity = 45/116 (38.79%), Postives = 66/116 (56.90%), Query Frame = 2

Query: 1292 YYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEAGTFGYTLELVNLRDTLASVLGMGCEQ*W 1639
            +YLG   SGAP HIH+DA N  + G+K+W++  P  A WS +   E+  +S  G A  +  P+ECVQ  GD++YVP  WGH+ +N+EA + G   E  +    L  V  +   + W
Sbjct:    4 FYLGPPGSGAPMHIHNDAANYLIYGRKRWFLSPPGDAEWSVQPASEF-RDSLAGLASDQHAPIECVQQAGDMLYVPKGWGHSTINIEA-SVGVAYEFTHAAGLLDPVGRLSGRRGW 117          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A812KZU7_9DINO (JMJD8 protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812KZU7_9DINO)

HSP 1 Score: 96.3 bits (238), Expect = 7.860e-17
Identity = 67/200 (33.50%), Postives = 94/200 (47.00%), Query Frame = 2

Query:  977 AEAWGHRAVDVGGVPYAKIFDLFNER---TTLNDYIDSTARAAAAVAADREDPAARLSPNYVF-QVDTEACIEGRELFERIVEAALPSSGDKPI--VCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECV--QHGGDLVYVPGDWGHAAMNLE 1552
            A+A G     V  +PY   F L   +   TTL D++ +T +  A     RE  A R    Y F  VD E   +   L E  +  A+      P   V P         ++ + +GG+ SG+P H H DALNL + G+K+WW+V P  A +SR H  +  +      +  +     CV  Q  GD+VYVP  WGHA +NLE
Sbjct:  289 ADAAGEARAMVSEIPYGADFGLDPSQETPTTLRDFLQATPQPPAKRQRCREQQADRAYRPYFFVAVDKELQPQLARLLEAKLGVAVGGRVSWPFGEVQPAQSLPFTATTIQFAVGGEGSGSPMHFHQDALNLCLRGRKRWWLVPPSEAAFSRLHPLDALDMGTSANSARDCLEGACVFEQEEGDVVYVPDMWGHAVLNLE 488          

BLAST of mRNA_C-linearis_contig102.778.1 vs. uniprot
Match: A0A7S3BH53_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3BH53_9EUKA)

HSP 1 Score: 90.9 bits (224), Expect = 4.080e-16
Identity = 59/194 (30.41%), Postives = 91/194 (46.91%), Query Frame = 2

Query:  974 FAEAWGHRAVDVGGVPYAKIFDLFNERTTLNDYIDSTARAAAAVAADREDPAARLSPNYVFQVDTEACIEGRELFERIVEAALPSSGDKPIVCPPPGGLRGLESVHYYLGGQWSGAPFHIHSDALNLAVSGKKKWWVVTPRSAVWSRRHIREYAEESKGGPAGGEDRPMECVQHGGDLVYVPGDWGHAAMNLEA 1555
            F   +G + V VG +PY  ++       ++  Y++      +A  A R   +A   P Y+F  D    +E        V   L   G  P +          +S  +YLG   +GAP H H+DA N  + G+K+W++  P  A WS +   E+        A  +  P+ECVQH GD++YVP  WGH+ +N+EA
Sbjct:   84 FLSKYGSQDVTVGRIPYGGLYGQEMRTMSMAQYVEYVVDDVSA--APRNYGSA---PPYLFFADA-PWLEADTAPSPTVLRRLSFHGQPPEI----------QSHQFYLGPPETGAPVHFHNDAANYLMYGRKRWFLYPPGDAEWSLQPASEFMHSV----ALEQGAPIECVQHSGDMLYVPKGWGHSTINIEA 257