mRNA_A-nodosum_M_contig10.120.1 (mRNA) Ascophyllum nodosum dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_A-nodosum_M_contig10.120.1
Unique NamemRNA_A-nodosum_M_contig10.120.1
TypemRNA
OrganismAscophyllum nodosum dioecious (Ascophyllum nodosum dioecious (Feamainn bhuX, rockweed, Norwegian kelp, knotted kelp, knotted wrack or egg wrack))
Homology
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BLAST of mRNA_A-nodosum_M_contig10.120.1 vs. uniprot
Match: A0A6H5L2L7_9PHAE (USP domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2L7_9PHAE)

HSP 1 Score: 223 bits (568), Expect = 4.470e-59
Identity = 197/559 (35.24%), Postives = 254/559 (45.44%), Query Frame = 1 
Query: 1240 ANDFRVSEAEGAGVAGNTGES-------VLIAVDNLLKEVDERNRALL------AGGFDDSNVQKEAQHTRTIQMVDWEGSLTLGVRPRDEIPLGNVKNLRNGDTHMNVVVPETLVKLVREGWLSASRSR-GDSWAGNAPRARGLEGISTNFGCGQECVSELFGKLLDLGISHV--------------DGEAHGIA-TRLSQAFRGSVCTQTLCVECERDRLDRDTFTELVLPPLLPLPSSSDHEGHKPSAVKSTSSPKSL-----TLLELVKATLGSESLVGNNKVWCQFCRQQGEAERRSTLHCSPRLLALHLRPATDEISDR---PVESAWASAPGSLKAMPENRHCVRSVDSKL--------IERVMAIQGASMCQFHETTRAEAGAAKEASYTDQEEGATVDDRRGKHEKKKQLPLPDPSFEVVYDLVGIILHQGRSMGSGHYTFARHVLHDHAPSRRATISDSGTGRPSVIEKSDNRRVEDSNDAQYFSFQDCQGQDGRENSQSRL-PPDENFVIFDDACVRFLSPEEERILLNGGGSSWGFGDAFLVFYARR 2778
            A D   + A  AG A   G         VL+A++ +L E++ RNRAL       AG     +   E + T +      E          D+ PL    +   G   +  + P +LV+L+  GWLSA R+R G   AG           ST+FG GQ CVSEL GK L L +                  G  H  +   L+QAFRG++C +TLCVECERDR  R+ FTEL LPPLLP   +      +           SL     T+  L+      ESL GNNKVWC+ CRQ  EAERRS++H  P LLALH+RP T + S     P   A AS       + + R   R  D +L        IER + ++ A+ CQFH       G              +VD   G+        L D S ++ YDL+G ILHQG+++ SGHYTFA +     A    A+               DNR +              QG  G   + S    PD  F +FDDA VR+LS EEER +L G G   GFGD FLVFYARR
Sbjct:   30 AADAAPAGAALAGTAAPVGRETGTQRNPVLVALEQVLGEMETRNRALFQQQLVAAGSQTAVSPPAEPESTSSGATSSAESKTCQWF---DQTPLRPSASTGEG-VRVESIAPTSLVELIHGGWLSADRARLGGGGAGTTG--------STDFGTGQACVSELLGKFLGLVVDDXXXXXXXXXXXXXXRSGGTHSSSFCGLAQAFRGTLCARTLCVECERDRTAREDFTELTLPPLLPPQHAPPLTAQRAIPAAENDGGPSLRGRHRTIQTLIDGVFERESLAGNNKVWCEACRQWTEAERRSSVHSPPGLLALHVRPGTRKRSSPCLYPKALARASGGAPDSVVGDVR---RGADGRLPAGSREEAIERTLVVRKAARCQFHG-----GGVNSXXXXXXXXXXXSVDSCTGQ--------LCD-SGDIFYDLIGAILHQGQTLASGHYTFAINA----AAGDWASFLAQQXXXXXXXADRDNRALVQ------------QGPPGATAAGSTYGKPD--FALFDDATVRWLSLEEERAVLRGDGG--GFGDPFLVFYARR 539
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BLAST of mRNA_A-nodosum_M_contig10.120.1 vs. uniprot
Match: D7FM48_ECTSI (USP domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FM48_ECTSI)

HSP 1 Score: 192 bits (488), Expect = 3.350e-48
Identity = 228/757 (30.12%), Postives = 292/757 (38.57%), Query Frame = 1 
Query:  628 APSGYTRKSL-------PVQRSGSWVGGSRQARLRLSLEKRRALPVPQLEATLSTASIDRKHGDGSESAPQGYPKSVPFQQQSRSKFEAPGALP----ASGVP---NLGRHDFNLNRSSVVASERASALPRDQTSTPPPPPFVGLVNLGETCYINAVVQALVACSRAILSSTGRCSPSDTAHVMGSNRTCGESGVPAASRQGSNGEKEVVLWSAAIVDANDFRVSEAEGAGVAGNTGESVLIAVDNLLKEVDERNRAL----LAGGFDDSNVQKEAQHTRTIQMVDWEGSLTLGVRPRDEIPLGNVKNLRNGDTHMNVVVPETLVKLVREGWLSASRSRGDSWAGNAPRARGLEGISTNFGCGQECVSELFGKLLDL----------GISHVDGEAHGIATR-----LSQAFRGSVCTQTLCVECERDRLDRDTFTELVLPPLLPLPSSSDH---EGHKPSAVKSTSSP----KSLTLLELVKATLGSESLVGNNKVWCQFCRQQGEAERRSTLHCSPRLLALHLRPATDEISDRPVESAWASAPGSLKAMPENRHCVRSVDSKLIERVMAIQGASMCQFHETTRAEAGAAKEASYTDQEEGATVDDRRGKHEKKKQLPLPDPSFEVVYDLVGIILHQGRSMGSGHYTFARHVLHDHAPSRRATISDSGTGRPSVIEKSDNRRVEDSNDAQYFSFQDCQGQDGRENSQSRLPPDENFVIFDDACVRFLSPEEERILLNGGGSSWGFGDAFLVFYARR 2778
            APS  +R ++       P   S S V   R+ R RL L KRR   +P   A +   SI             G   S  FQ++ RS F +  AL     A G P   + G  +  L+R+ V+A    +A   ++ S+ P PPFVGL NLGETCY+NAV+QAL AC   + SS G           GS  +C       A+  G+  E   VL                  AGV G     VL A++ +L E++ RNRAL    L      + V   A+   T          +   +P D+ PL    +   G   +  + P  LV+L+R GWLSA RSR     G    A G    ST FG GQ CVSEL GK L L           +  V G   G +       L+QAFRG++C +TLCVECERDR  R+ FTEL LPPLLP P  +     +G  P+A ++   P    +  T+  LV      ESL GNNK                                                                                +C                                             S +V YDL+G+ILHQG+++ SGHYTFA H          A   DS     S +E                         G   S    P    F +FDDA V +LSPEEER +L GGG    FGD FLVFYARR
Sbjct:   14 APSAQSRAAVLFGAENHPPVGSSSGVNRLRK-RSRLCLGKRRRA-LPAQHAAVGQPSI-------------GTAGSPSFQERFRSPFTSSAALAPPAEAIGYPWGASPGLSETCLDRAGVLAP---AAWTIERPSSGPSPPFVGLKNLGETCYVNAVLQALTACHGVLTSSRGGGGGWHDVGSEGSEGSCSTPPAADAAPAGAASEGTAVLV-----------------AGVTGTQRNPVLAALEQVLGEMETRNRALSQQQLVAFGSQTAVSSPAEPESTGSGAT-SSPESKTCQPYDQTPLRPSASTGEG-VLVEAIAPTFLVELIRGGWLSADRSR---LGGGGAGATG----STEFGTGQACVSELLGKFLGLVVDDGGSGATSLGGVGGRRSGGSHSSSFCCLAQAFRGTLCARTLCVECERDRTAREDFTELTLPPLLPPPQHAPPLTAQGAIPAA-ENDGGPSLRGRRRTMQTLVDGVFEGESLAGNNK--------------------------------------------------------------------------------LCD--------------------------------------------SGDVFYDLIGVILHQGQTLASGHYTFALH----------AAAGDSA----SFLEXXXXXXXXXXXXXXXXXXXXXXXXXG---STCGKP---GFALFDDATVGWLSPEEERAVLRGGGGV--FGDPFLVFYARR 579
The following BLAST results are available for this feature:
BLAST of mRNA_A-nodosum_M_contig10.120.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5L2L7_9PHAE4.470e-5935.24USP domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FM48_ECTSI3.350e-4830.12USP domain-containing protein n=1 Tax=Ectocarpus s... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
A-nodosum_M_contig10contigA-nodosum_M_contig10:1116439..1119851 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ascophyllum nodosum dioecious OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog144197.XP_008286375.1
Preferred nameUSP1
PFAMsUCH
Model size3413
Max annot lvl33208|Metazoa
KEGG koko:K11832
KEGG Pathwayko03460,map03460
Hectar predicted targeting categoryother localisation
GOsGO:0001501,GO:0003674,GO:0003824,GO:0004175,GO:0004197,GO:0004843,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0006282,GO:0006464,GO:0006508,GO:0006807,GO:0006950,GO:0006974,GO:0007275,GO:0008150,GO:0008152,GO:0008233,GO:0008234,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009987,GO:0016579,GO:0016787,GO:0019219,GO:0019222,GO:0019538,GO:0019783,GO:0031323,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0033554,GO:0035520,GO:0036211,GO:0036459,GO:0042769,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0048583,GO:0048731,GO:0048856,GO:0050789,GO:0050794,GO:0050896,GO:0051052,GO:0051171,GO:0051606,GO:0051716,GO:0060255,GO:0065007,GO:0070011,GO:0070013,GO:0070646,GO:0070647,GO:0071704,GO:0080090,GO:0080134,GO:0080135,GO:0101005,GO:0140096,GO:1901564,GO:2001020
Exons1
Evalue2.35e-06
EggNOG OGsKOG1864@1|root,KOG1864@2759|Eukaryota,38DYD@33154|Opisthokonta,3BAN8@33208|Metazoa,3D0YD@33213|Bilateria,4844F@7711|Chordata,493AG@7742|Vertebrata,49R17@7898|Actinopterygii
Ec32 ortholog descriptionPeptidase C19, ubiquitin carboxyl-terminal hydrolase
Ec32 orthologEc-01_010630.1
EC3.4.19.12
DescriptionUbiquitin specific protease 1
Cds size2634
COG categoryO
BRITEko00000,ko00001,ko01000,ko01002,ko03400,ko04121
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866352.7944446-UTR-A-nodosum_M_contig10:1116438..11165911680866352.7944446-UTR-A-nodosum_M_contig10:1116438..1116591Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig10 1116439..1116591 +
1680866352.8580341-UTR-A-nodosum_M_contig10:1119225..11198511680866352.8580341-UTR-A-nodosum_M_contig10:1119225..1119851Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig10 1119226..1119851 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866352.8468628-CDS-A-nodosum_M_contig10:1116591..11192251680866352.8468628-CDS-A-nodosum_M_contig10:1116591..1119225Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig10 1116592..1119225 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_A-nodosum_M_contig10.120.1prot_A-nodosum_M_contig10.120.1Ascophyllum nodosum dioeciouspolypeptideA-nodosum_M_contig10 1116592..1119225 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_A-nodosum_M_contig10.120.1

>prot_A-nodosum_M_contig10.120.1 ID=prot_A-nodosum_M_contig10.120.1|Name=mRNA_A-nodosum_M_contig10.120.1|organism=Ascophyllum nodosum dioecious|type=polypeptide|length=878bp
MSEGAQPSQQVRSSTTTNTRSNGHLKRPRSVTPTKPSGILPAENPIPRYG
TRSTTKVRQRRQSIIAQLPTQQSEFSRPGKNVAAADTILSSNTRVLDGGV
STPLSTGGEREALGTFPPKGSSTANASEAAVTESASVSSVAPIVRTIDPT
AGCLGPSLAPSGYTRKSLPVQRSGSWVGGSRQARLRLSLEKRRALPVPQL
EATLSTASIDRKHGDGSESAPQGYPKSVPFQQQSRSKFEAPGALPASGVP
NLGRHDFNLNRSSVVASERASALPRDQTSTPPPPPFVGLVNLGETCYINA
VVQALVACSRAILSSTGRCSPSDTAHVMGSNRTCGESGVPAASRQGSNGE
KEVVLWSAAIVDANDFRVSEAEGAGVAGNTGESVLIAVDNLLKEVDERNR
ALLAGGFDDSNVQKEAQHTRTIQMVDWEGSLTLGVRPRDEIPLGNVKNLR
NGDTHMNVVVPETLVKLVREGWLSASRSRGDSWAGNAPRARGLEGISTNF
GCGQECVSELFGKLLDLGISHVDGEAHGIATRLSQAFRGSVCTQTLCVEC
ERDRLDRDTFTELVLPPLLPLPSSSDHEGHKPSAVKSTSSPKSLTLLELV
KATLGSESLVGNNKVWCQFCRQQGEAERRSTLHCSPRLLALHLRPATDEI
SDRPVESAWASAPGSLKAMPENRHCVRSVDSKLIERVMAIQGASMCQFHE
TTRAEAGAAKEASYTDQEEGATVDDRRGKHEKKKQLPLPDPSFEVVYDLV
GIILHQGRSMGSGHYTFARHVLHDHAPSRRATISDSGTGRPSVIEKSDNR
RVEDSNDAQYFSFQDCQGQDGRENSQSRLPPDENFVIFDDACVRFLSPEE
ERILLNGGGSSWGFGDAFLVFYARRDS*
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mRNA from alignment at A-nodosum_M_contig10:1116439..1119851+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_A-nodosum_M_contig10.120.1 ID=mRNA_A-nodosum_M_contig10.120.1|Name=mRNA_A-nodosum_M_contig10.120.1|organism=Ascophyllum nodosum dioecious|type=mRNA|length=3413bp|location=Sequence derived from alignment at A-nodosum_M_contig10:1116439..1119851+ (Ascophyllum nodosum dioecious)
TGAAATCTCGTCCTCCTTTCTGTAGATAGAGGCCCTGGGGATAGCTCCTC
CTCCTTGAAATTTTGGGAATTTTTTTGAGGTCCTGTACTGGCTTCCAGGA
GAGCTCTGCTTGGAAGGGAAGAAGGGCGGCGTCGCTCTATCCTTTATTGG
CGGATGAGTGAGGGTGCCCAACCGTCTCAGCAAGTTCGTTCTTCTACGAC
GACAAACACGCGGAGTAACGGCCATCTGAAGCGCCCTAGATCAGTTACAC
CCACCAAGCCCAGTGGAATCCTACCTGCTGAAAATCCCATTCCTCGGTAT
GGTACGAGATCGACCACAAAAGTGCGTCAGAGAAGACAATCGATCATCGC
CCAACTCCCCACCCAGCAGTCTGAATTTAGTCGGCCTGGGAAAAACGTAG
CCGCTGCTGACACAATCCTTTCGTCTAATACACGAGTACTGGACGGAGGA
GTGTCAACGCCTCTCAGTACAGGAGGAGAGCGGGAAGCTCTGGGAACGTT
TCCTCCTAAAGGCTCTAGTACTGCTAACGCATCTGAAGCGGCGGTAACAG
AGAGCGCCAGTGTTTCATCGGTTGCTCCAATAGTAAGGACGATCGATCCT
ACCGCAGGATGTCTGGGCCCCTCCCTCGCACCTTCAGGATATACGCGGAA
ATCGCTACCGGTACAAAGAAGCGGGAGTTGGGTTGGAGGTAGTCGTCAGG
CAAGATTGAGGCTCAGCCTGGAAAAGCGTCGCGCGTTGCCCGTGCCGCAA
CTGGAGGCTACCTTGTCAACCGCGTCGATTGATCGCAAGCACGGTGATGG
ATCCGAGTCCGCGCCGCAGGGCTATCCAAAGAGCGTTCCGTTCCAGCAGC
AAAGCCGCTCGAAGTTCGAAGCGCCTGGGGCCCTACCCGCTTCTGGGGTC
CCGAACCTTGGGCGCCACGATTTCAATTTGAATCGATCGTCGGTAGTGGC
CTCTGAGCGCGCCTCGGCTTTACCTCGCGATCAGACGTCAACACCACCGC
CGCCGCCGTTTGTGGGGTTAGTCAACCTAGGCGAAACCTGCTACATAAAT
GCCGTGGTGCAAGCGCTGGTGGCCTGCTCTCGAGCGATACTATCATCAAC
AGGGAGATGCAGTCCGAGCGACACTGCGCACGTTATGGGAAGCAACAGGA
CCTGCGGCGAGTCAGGCGTGCCAGCGGCGTCACGCCAAGGATCAAATGGC
GAGAAAGAAGTCGTTCTTTGGTCCGCGGCTATCGTCGATGCTAACGACTT
CAGAGTGAGTGAAGCAGAAGGGGCAGGGGTTGCTGGTAACACGGGGGAAT
CGGTATTGATCGCCGTGGATAATTTGTTAAAAGAAGTGGATGAACGCAAC
AGAGCATTGTTAGCGGGAGGTTTTGACGATAGCAACGTTCAGAAAGAGGC
TCAACATACGCGCACCATCCAGATGGTGGACTGGGAGGGGTCTTTGACGT
TGGGAGTACGGCCACGAGACGAGATACCGCTGGGCAATGTCAAAAACCTC
AGGAACGGAGACACCCACATGAATGTCGTCGTTCCAGAAACGTTGGTCAA
GCTCGTGCGTGAGGGCTGGCTCAGCGCTAGCCGCTCAAGAGGAGATAGTT
GGGCTGGCAATGCGCCGCGAGCCAGAGGACTCGAAGGCATTTCAACGAAT
TTCGGGTGCGGACAAGAATGCGTCTCCGAGCTTTTTGGTAAGCTCTTGGA
TCTCGGGATTTCTCACGTGGACGGGGAGGCCCATGGCATCGCCACCCGCT
TGAGCCAAGCTTTCCGCGGCTCGGTATGTACTCAAACGCTCTGCGTGGAG
TGCGAGCGGGACAGACTCGACCGCGATACCTTCACAGAGCTCGTTCTGCC
TCCACTGTTACCTCTGCCTTCATCTTCAGACCACGAGGGCCATAAGCCAT
CTGCAGTGAAGAGCACTTCATCGCCCAAGAGCCTGACGTTATTGGAACTC
GTGAAGGCGACGCTAGGGAGCGAATCACTCGTGGGAAACAACAAGGTTTG
GTGCCAGTTTTGCCGCCAGCAGGGGGAGGCCGAGCGCCGATCTACCCTCC
ACTGCTCTCCACGTTTGCTTGCCCTTCATCTTCGACCAGCGACTGACGAA
ATTTCGGATCGGCCAGTCGAGAGCGCTTGGGCGTCCGCCCCCGGGTCACT
TAAAGCGATGCCTGAGAACAGGCATTGCGTTAGAAGCGTCGACTCAAAGC
TGATCGAGCGTGTGATGGCCATCCAAGGTGCTTCTATGTGCCAGTTTCAC
GAGACGACGCGGGCAGAGGCAGGTGCAGCAAAGGAAGCTTCGTACACCGA
CCAGGAGGAAGGAGCAACAGTGGACGATCGAAGAGGTAAGCACGAAAAAA
AAAAACAGTTGCCGCTCCCGGATCCTTCGTTCGAGGTTGTTTACGACCTC
GTCGGCATCATACTGCACCAGGGCCGATCGATGGGGTCTGGTCACTACAC
CTTCGCACGCCACGTACTCCACGATCACGCGCCCAGTCGCAGAGCAACTA
TCAGTGACAGCGGGACCGGACGGCCTTCAGTAATAGAGAAGAGTGATAAC
AGGCGTGTAGAGGATAGTAATGATGCGCAATATTTTTCGTTTCAAGATTG
CCAAGGGCAGGATGGGCGGGAGAATAGTCAGTCACGACTTCCCCCTGATG
AGAACTTTGTGATTTTCGACGACGCTTGCGTACGATTTTTATCGCCCGAG
GAGGAACGAATTCTCTTAAATGGCGGTGGCTCGTCGTGGGGTTTCGGCGA
CGCTTTCTTGGTGTTCTATGCGCGGCGGGACTCGTGACGCTCAGCACAAA
ACCGCACGCACGAAGCATACCAAACCGTTCGCAAAAACGTTTGCTAGCAT
GAGGTGTGCTGTTTGAAGTTCAGTCGGTTGTGGTGTGCTGGTATGTTTTT
GTTTTGTATTATTGTAGGCGGATTGTTGCTGCACAATTTAGGCCTGCACA
TTTCCTGTGCTTTTCCTCTTAACAATGTTCTTTCTAACGACGCTGGATGT
CGAGCTACCTTCTCGATTTGCCCAAGTACCTCCTTGTTCCTGCAACAGAC
AGACGCTTTTCGTAGTTCTCTGTATTACAATTAACCTGGCAGATAAAATG
TCGTCTGCTCGGCCTGAAGTCAAGCCAAGGCATCCTCTTGATTCGTGTTC
TGGCGTTGGTTTCTTTGTAGCAATAGTGACATGTTCTGTACTGTACTCAA
GTACGTCGGAGTTTGTTAACGCTTTCCCCAGTCATTTATTTTTTCTCGTT
TTGCGTAGGGCTTGTTTTCCAGGTTATCGATCTCAATCCCGGTTGCAGTT
CGCTGAATGGGAAAAAAATATTGGAAATGAGGCAAATAATCCACGACAGA
ATTAACAATTCTTGACGTTGGCTTTTTATTTTTCATCATTTTTTTTTATT
GTATATGCAGCAC
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Coding sequence (CDS) from alignment at A-nodosum_M_contig10:1116439..1119851+

>mRNA_A-nodosum_M_contig10.120.1 ID=mRNA_A-nodosum_M_contig10.120.1|Name=mRNA_A-nodosum_M_contig10.120.1|organism=Ascophyllum nodosum dioecious|type=CDS|length=2634bp|location=Sequence derived from alignment at A-nodosum_M_contig10:1116439..1119851+ (Ascophyllum nodosum dioecious)
ATGAGTGAGGGTGCCCAACCGTCTCAGCAAGTTCGTTCTTCTACGACGAC
AAACACGCGGAGTAACGGCCATCTGAAGCGCCCTAGATCAGTTACACCCA
CCAAGCCCAGTGGAATCCTACCTGCTGAAAATCCCATTCCTCGGTATGGT
ACGAGATCGACCACAAAAGTGCGTCAGAGAAGACAATCGATCATCGCCCA
ACTCCCCACCCAGCAGTCTGAATTTAGTCGGCCTGGGAAAAACGTAGCCG
CTGCTGACACAATCCTTTCGTCTAATACACGAGTACTGGACGGAGGAGTG
TCAACGCCTCTCAGTACAGGAGGAGAGCGGGAAGCTCTGGGAACGTTTCC
TCCTAAAGGCTCTAGTACTGCTAACGCATCTGAAGCGGCGGTAACAGAGA
GCGCCAGTGTTTCATCGGTTGCTCCAATAGTAAGGACGATCGATCCTACC
GCAGGATGTCTGGGCCCCTCCCTCGCACCTTCAGGATATACGCGGAAATC
GCTACCGGTACAAAGAAGCGGGAGTTGGGTTGGAGGTAGTCGTCAGGCAA
GATTGAGGCTCAGCCTGGAAAAGCGTCGCGCGTTGCCCGTGCCGCAACTG
GAGGCTACCTTGTCAACCGCGTCGATTGATCGCAAGCACGGTGATGGATC
CGAGTCCGCGCCGCAGGGCTATCCAAAGAGCGTTCCGTTCCAGCAGCAAA
GCCGCTCGAAGTTCGAAGCGCCTGGGGCCCTACCCGCTTCTGGGGTCCCG
AACCTTGGGCGCCACGATTTCAATTTGAATCGATCGTCGGTAGTGGCCTC
TGAGCGCGCCTCGGCTTTACCTCGCGATCAGACGTCAACACCACCGCCGC
CGCCGTTTGTGGGGTTAGTCAACCTAGGCGAAACCTGCTACATAAATGCC
GTGGTGCAAGCGCTGGTGGCCTGCTCTCGAGCGATACTATCATCAACAGG
GAGATGCAGTCCGAGCGACACTGCGCACGTTATGGGAAGCAACAGGACCT
GCGGCGAGTCAGGCGTGCCAGCGGCGTCACGCCAAGGATCAAATGGCGAG
AAAGAAGTCGTTCTTTGGTCCGCGGCTATCGTCGATGCTAACGACTTCAG
AGTGAGTGAAGCAGAAGGGGCAGGGGTTGCTGGTAACACGGGGGAATCGG
TATTGATCGCCGTGGATAATTTGTTAAAAGAAGTGGATGAACGCAACAGA
GCATTGTTAGCGGGAGGTTTTGACGATAGCAACGTTCAGAAAGAGGCTCA
ACATACGCGCACCATCCAGATGGTGGACTGGGAGGGGTCTTTGACGTTGG
GAGTACGGCCACGAGACGAGATACCGCTGGGCAATGTCAAAAACCTCAGG
AACGGAGACACCCACATGAATGTCGTCGTTCCAGAAACGTTGGTCAAGCT
CGTGCGTGAGGGCTGGCTCAGCGCTAGCCGCTCAAGAGGAGATAGTTGGG
CTGGCAATGCGCCGCGAGCCAGAGGACTCGAAGGCATTTCAACGAATTTC
GGGTGCGGACAAGAATGCGTCTCCGAGCTTTTTGGTAAGCTCTTGGATCT
CGGGATTTCTCACGTGGACGGGGAGGCCCATGGCATCGCCACCCGCTTGA
GCCAAGCTTTCCGCGGCTCGGTATGTACTCAAACGCTCTGCGTGGAGTGC
GAGCGGGACAGACTCGACCGCGATACCTTCACAGAGCTCGTTCTGCCTCC
ACTGTTACCTCTGCCTTCATCTTCAGACCACGAGGGCCATAAGCCATCTG
CAGTGAAGAGCACTTCATCGCCCAAGAGCCTGACGTTATTGGAACTCGTG
AAGGCGACGCTAGGGAGCGAATCACTCGTGGGAAACAACAAGGTTTGGTG
CCAGTTTTGCCGCCAGCAGGGGGAGGCCGAGCGCCGATCTACCCTCCACT
GCTCTCCACGTTTGCTTGCCCTTCATCTTCGACCAGCGACTGACGAAATT
TCGGATCGGCCAGTCGAGAGCGCTTGGGCGTCCGCCCCCGGGTCACTTAA
AGCGATGCCTGAGAACAGGCATTGCGTTAGAAGCGTCGACTCAAAGCTGA
TCGAGCGTGTGATGGCCATCCAAGGTGCTTCTATGTGCCAGTTTCACGAG
ACGACGCGGGCAGAGGCAGGTGCAGCAAAGGAAGCTTCGTACACCGACCA
GGAGGAAGGAGCAACAGTGGACGATCGAAGAGGTAAGCACGAAAAAAAAA
AACAGTTGCCGCTCCCGGATCCTTCGTTCGAGGTTGTTTACGACCTCGTC
GGCATCATACTGCACCAGGGCCGATCGATGGGGTCTGGTCACTACACCTT
CGCACGCCACGTACTCCACGATCACGCGCCCAGTCGCAGAGCAACTATCA
GTGACAGCGGGACCGGACGGCCTTCAGTAATAGAGAAGAGTGATAACAGG
CGTGTAGAGGATAGTAATGATGCGCAATATTTTTCGTTTCAAGATTGCCA
AGGGCAGGATGGGCGGGAGAATAGTCAGTCACGACTTCCCCCTGATGAGA
ACTTTGTGATTTTCGACGACGCTTGCGTACGATTTTTATCGCCCGAGGAG
GAACGAATTCTCTTAAATGGCGGTGGCTCGTCGTGGGGTTTCGGCGACGC
TTTCTTGGTGTTCTATGCGCGGCGGGACTCGTGA
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