mRNA_13304 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|178167
Unique NamemRNA_13304
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A835ZD90_9STRA (Peptidylprolyl isomerase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZD90_9STRA)

HSP 1 Score: 236 bits (603), Expect = 3.820e-79
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN 336
            MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN
Sbjct:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN 112
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A7J7LNC2_9MAGN (Peptidylprolyl isomerase n=1 Tax=Kingdonia uniflora TaxID=39325 RepID=A0A7J7LNC2_9MAGN)

HSP 1 Score: 193 bits (491), Expect = 4.660e-62
Identity = 88/112 (78.57%), Postives = 96/112 (85.71%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN 336
            MG EK+++K GNG KP +GQSVTVHCTGFGKN DL+ KFWSTKDPGQQPFTFNIGLGSVIK WDEGVMGM +GE A +  SPDY YG GGFPAWGI PNSVL F+IEVLSIN
Sbjct:    1 MGVEKQIVKVGNGSKPIKGQSVTVHCTGFGKNGDLSQKFWSTKDPGQQPFTFNIGLGSVIKGWDEGVMGMQLGEVARLQCSPDYGYGPGGFPAWGIQPNSVLVFEIEVLSIN 112
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: M7Z599_TRIUA (Peptidylprolyl isomerase n=1 Tax=Triticum urartu TaxID=4572 RepID=M7Z599_TRIUA)

HSP 1 Score: 193 bits (491), Expect = 4.660e-62
Identity = 87/110 (79.09%), Postives = 97/110 (88.18%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLS 330
            MGFEKE+LKAG GPKP +GQ VTVHCTG+GK+ DL+ KFWSTKDPGQQPF+FNIGLGSVIK WDEGVMGM +GE A +T +PDYAYG GGFPAWGI PNSVL F+IEVLS
Sbjct:    1 MGFEKEILKAGTGPKPVKGQKVTVHCTGYGKDGDLSKKFWSTKDPGQQPFSFNIGLGSVIKGWDEGVMGMQLGEGARLTCTPDYAYGEGGFPAWGIQPNSVLIFEIEVLS 110
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A3B6PP75_WHEAT (Peptidylprolyl isomerase n=4 Tax=Triticinae TaxID=1648030 RepID=A0A3B6PP75_WHEAT)

HSP 1 Score: 193 bits (491), Expect = 1.440e-61
Identity = 87/110 (79.09%), Postives = 97/110 (88.18%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLS 330
            MGFEKE+LKAG GPKP +GQ VTVHCTG+GK+ DL+ KFWSTKDPGQQPF+FNIGLGSVIK WDEGVMGM +GE A +T +PDYAYG GGFPAWGI PNSVL F+IEVLS
Sbjct:   36 MGFEKEILKAGTGPKPVKGQKVTVHCTGYGKDGDLSKKFWSTKDPGQQPFSFNIGLGSVIKGWDEGVMGMQLGEVARLTCTPDYAYGTGGFPAWGIQPNSVLIFEIEVLS 145
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A453PLS3_AEGTS (Peptidylprolyl isomerase n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453PLS3_AEGTS)

HSP 1 Score: 194 bits (492), Expect = 4.320e-61
Identity = 87/110 (79.09%), Postives = 98/110 (89.09%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLS 330
            MGFEKE+LKAG GPKP +GQ VTVHCTG+GK+ DL+ KFWSTKDPGQQPF+FNIGLGSVIK WDEGVMGM +GE A +T +PDYAYG+GGFPAWGI PNSVL F+IEVLS
Sbjct:   81 MGFEKEILKAGTGPKPVKGQKVTVHCTGYGKDGDLSKKFWSTKDPGQQPFSFNIGLGSVIKGWDEGVMGMQLGEVARLTCTPDYAYGSGGFPAWGIQPNSVLIFEIEVLS 190
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A0F7CZ54_9ASPA (Peptidylprolyl isomerase n=2 Tax=Mesangiospermae TaxID=1437183 RepID=A0A0F7CZ54_9ASPA)

HSP 1 Score: 191 bits (484), Expect = 5.270e-61
Identity = 86/111 (77.48%), Postives = 97/111 (87.39%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSI 333
            MG EK++LKAGNGPKP RGQ+VTVHCTG+GK+ DL+ KFWSTKDPGQQPFTF IGLGSVIK WDEGV+ M +GE A +  SPDYAYGAGGFPAWGI PNSVL F+IEVLS+
Sbjct:    1 MGVEKQILKAGNGPKPVRGQNVTVHCTGYGKDGDLSKKFWSTKDPGQQPFTFKIGLGSVIKGWDEGVLDMQLGEIARLRCSPDYAYGAGGFPAWGIQPNSVLVFEIEVLSV 111
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A3B6PQ18_WHEAT (Peptidylprolyl isomerase n=16 Tax=Triticinae TaxID=1648030 RepID=A0A3B6PQ18_WHEAT)

HSP 1 Score: 193 bits (491), Expect = 6.750e-61
Identity = 87/110 (79.09%), Postives = 97/110 (88.18%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLS 330
            MGFEKE+LKAG GPKP +GQ VTVHCTG+GK+ DL+ KFWSTKDPGQQPF+FNIGLGSVIK WDEGVMGM +GE A +T +PDYAYG GGFPAWGI PNSVL F+IEVLS
Sbjct:   84 MGFEKEILKAGTGPKPVKGQKVTVHCTGYGKDGDLSKKFWSTKDPGQQPFSFNIGLGSVIKGWDEGVMGMQLGEVARLTCTPDYAYGTGGFPAWGIQPNSVLIFEIEVLS 193
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A8K1XRU2_9BRYO (Peptidyl-prolyl cis-trans isomerase FKBP12 n=1 Tax=Polytrichastrum alpinum TaxID=129212 RepID=A0A8K1XRU2_9BRYO)

HSP 1 Score: 189 bits (481), Expect = 1.560e-60
Identity = 84/111 (75.68%), Postives = 98/111 (88.29%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSI 333
            MGFEKE+++AGNG KPARG++VTVHCTGFGKN+DL+ KFWSTKDPGQQPF F IGLG+VIK WDEGV+ M +GE A +  SPDYAYG+GGFPAWGI PNSVL F+IEVLS+
Sbjct:    1 MGFEKEIIRAGNGIKPARGKTVTVHCTGFGKNRDLSQKFWSTKDPGQQPFQFKIGLGTVIKGWDEGVLSMDIGEVARLKCSPDYAYGSGGFPAWGIQPNSVLVFEIEVLSV 111
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A1U8B9A3_NELNU (Peptidylprolyl isomerase n=5 Tax=Mesangiospermae TaxID=1437183 RepID=A0A1U8B9A3_NELNU)

HSP 1 Score: 189 bits (479), Expect = 3.150e-60
Identity = 86/112 (76.79%), Postives = 94/112 (83.93%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN 336
            MG EK+VL+AGNGPKP  GQSVTVHCTGFGKN DL+ KFWSTKDPGQ+PFTF IG GSVIK WDEGVMGM +GE A +  SPDYAYG  GFPAWGI PNSVL F+IEVLS+ 
Sbjct:    1 MGVEKQVLRAGNGPKPVAGQSVTVHCTGFGKNGDLSQKFWSTKDPGQKPFTFKIGQGSVIKGWDEGVMGMQIGEVARLQCSPDYAYGPSGFPAWGIQPNSVLVFEIEVLSVQ 112
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BLAST of jgi.p|Trimin1|178167 vs. uniprot
Match: A0A804J172_MUSAM (Peptidylprolyl isomerase n=1 Tax=Musa acuminata subsp. malaccensis TaxID=214687 RepID=A0A804J172_MUSAM)

HSP 1 Score: 187 bits (476), Expect = 9.020e-60
Identity = 86/112 (76.79%), Postives = 95/112 (84.82%), Query Frame = 1 
Query:    1 MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPFTFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNSVLKFDIEVLSIN 336
            MG EK++LKAG+GPKP RGQSVTVHCTGFGK+ DL+ KFWSTKDPGQQPFTF +GLGSVIK WDEGVM M VGE A +  SPDYAYGA GFP WGI PNSVL F+IEVLS+ 
Sbjct:    1 MGVEKQLLKAGHGPKPVRGQSVTVHCTGFGKDGDLSKKFWSTKDPGQQPFTFKVGLGSVIKGWDEGVMDMQVGEVARLQCSPDYAYGADGFPTWGIRPNSVLIFEIEVLSVQ 112
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|178167 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835ZD90_9STRA3.820e-79100.00Peptidylprolyl isomerase n=1 Tax=Tribonema minus T... [more]
A0A7J7LNC2_9MAGN4.660e-6278.57Peptidylprolyl isomerase n=1 Tax=Kingdonia uniflor... [more]
M7Z599_TRIUA4.660e-6279.09Peptidylprolyl isomerase n=1 Tax=Triticum urartu T... [more]
A0A3B6PP75_WHEAT1.440e-6179.09Peptidylprolyl isomerase n=4 Tax=Triticinae TaxID=... [more]
A0A453PLS3_AEGTS4.320e-6179.09Peptidylprolyl isomerase n=1 Tax=Aegilops tauschii... [more]
A0A0F7CZ54_9ASPA5.270e-6177.48Peptidylprolyl isomerase n=2 Tax=Mesangiospermae T... [more]
A0A3B6PQ18_WHEAT6.750e-6179.09Peptidylprolyl isomerase n=16 Tax=Triticinae TaxID... [more]
A0A8K1XRU2_9BRYO1.560e-6075.68Peptidyl-prolyl cis-trans isomerase FKBP12 n=1 Tax... [more]
A0A1U8B9A3_NELNU3.150e-6076.79Peptidylprolyl isomerase n=5 Tax=Mesangiospermae T... [more]
A0A804J172_MUSAM9.020e-6076.79Peptidylprolyl isomerase n=1 Tax=Musa acuminata su... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_166contigContig_166:142893..144719 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|178167|e_gw1.166.82.1
BRITEko00000,ko01000
KEGG koko:K01802
EC5.2.1.8
GOsGO:0000413,GO:0003674,GO:0003755,GO:0003824,GO:0005488,GO:0005527,GO:0005528,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006457,GO:0006464,GO:0006807,GO:0008144,GO:0008150,GO:0008152,GO:0009987,GO:0016853,GO:0016859,GO:0018193,GO:0018208,GO:0019538,GO:0036211,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0061077,GO:0071704,GO:0097159,GO:0140096,GO:1901363,GO:1901564
Preferred nameFKBP12
DescriptionPeptidyl-prolyl cis-trans isomerase
COG categoryO
Max annot lvl35493|Streptophyta
EggNOG OGsCOG0545@1|root,KOG0544@2759|Eukaryota,37VEG@33090|Viridiplantae,3GINI@35493|Streptophyta,3KZZV@4447|Liliopsida,3II48@38820|Poales
Evalue7.79e-62
Seed ortholog4565.Traes_6DL_565B3D7EC.2
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionFKBP domain protein
Ec32 orthologEc-19_001050.1
TranscriptId178489
TrackFilteredModels1
ProteinId178167
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|178167gene_13304Tribonema minus UTEX_B_3156 geneContig_166 142893..144719 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|178167Trimin1|178167|e_gw1.166.82.1Tribonema minus UTEX_B_3156 polypeptideContig_166 142893..144719 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_13304_4exon_13304_4Tribonema minus UTEX_B_3156 exonContig_166 142893..143017 -
exon_13304_3exon_13304_3Tribonema minus UTEX_B_3156 exonContig_166 143788..143891 -
exon_13304_2exon_13304_2Tribonema minus UTEX_B_3156 exonContig_166 144349..144395 -
exon_13304_1exon_13304_1Tribonema minus UTEX_B_3156 exonContig_166 144657..144719 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_13304CDS_13304Tribonema minus UTEX_B_3156 CDSContig_166 144657..144719 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_13304 ID=mRNA_13304|Name=jgi.p|Trimin1|178167|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=339bp|location=Sequence derived from alignment at Contig_166:142893..144719- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGGGCTTCGAGAAGGAGGTGCTTAAGGCGGGCAATGGACCCAAGCCGGC
CAGGGGACAATCGGTCACCGTTCACTGTACAGGATTTGGCAAGAATCAGG
ACCTCAACAGCAAATTCTGGAGCACTAAAGATCCCGGACAGCAGCCCTTC
ACATTCAACATAGGACTGGGCAGCGTCATCAAGGCGTGGGATGAAGGAGT
CATGGGCATGAGCGTCGGCGAACGAGCCATGATCACGGCCAGCCCTGACT
ATGCCTATGGTGCAGGCGGGTTTCCTGCATGGGGAATCATGCCCAACAGT
GTCCTCAAGTTTGACATTGAGGTCCTCAGCATCAACTGA
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protein sequence of jgi.p|Trimin1|178167

>Trimin1|178167|e_gw1.166.82.1 ID=Trimin1|178167|e_gw1.166.82.1|Name=jgi.p|Trimin1|178167|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=113bp
MGFEKEVLKAGNGPKPARGQSVTVHCTGFGKNQDLNSKFWSTKDPGQQPF
TFNIGLGSVIKAWDEGVMGMSVGERAMITASPDYAYGAGGFPAWGIMPNS
VLKFDIEVLSIN*
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mRNA from alignment at Contig_166:142893..144719-

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_13304 ID=mRNA_13304|Name=jgi.p|Trimin1|178167|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1827bp|location=Sequence derived from alignment at Contig_166:142893..144719- (Tribonema minus UTEX_B_3156 )
ATGGGCTTCGAGAAGGAGGTGCTTAAGGCGGGCAATGGACCCAAGCCGGC
CAGGGGACAATCGGTACGCAGAAAAAGCAGAAGCTGTGGCTTGTTCCAGC
CGCATCCAAGATCTCTCGAATCATTCAGTGGCCGTAGATTGCCCTGTCAC
TCACGCTTGCTTTCATTGCCGCCTGATGCCGTCGGCGACAGTTCGCAAAC
GCATGCATGCAGGCTGTTGTCGCGTAGATCACATGCGTTAATGTTGCACC
TATTTGGACTTGAATCTCCGACCTCATGGTGCTCATGTTGCTGATGGCGG
TTTCTGTGTGATGCCAACTGCCAGGTCACCGTTCACTGTACAGGATTTGG
CAAGAATCAGGACCTCAACAGGTACTAAGAAATCTGCGTTTCTATGATAA
GCTGGTGTCGCTTTCACGTTGAATGCGTGCATGTGTAAAATCATGCAGCC
TCCATGGCTGCATAAGTCATGCTTACGGACAGAGACCTGCCGATACTGAA
GCTTGACAGCAACATAAGAGTTCACAGATGTAACCATGCACTCTTACTAC
CCCTGTATGTGGCATGAGGGCTCAAACAGACTTGCTTTGCTGCCCTTGAG
CACCACAACACTGCTGTGCTGTTGTGCGAAGTTTTGCTGTGTTATTCAGA
TCGGCCAAATGCGTGCCCTGCACCAGGATGTATCTTGCGCGTCACAGAGC
GTGTGCACATTTGACGTTTCAAAAAAGTGAAGCCTCTGCACTGGCGACTT
TGAGTGATTCATCGTGTCCCTCACTGGATGCATGAGTGCCCACAACAACT
CGTCAATCCGCATGATGCATCTTCTCAGCAAATTCTGGAGCACTAAAGAT
CCCGGACAGCAGCCCTTCACATTCAACATAGGACTGGGCAGCGTCATCAA
GGCGTGGGATGAAGGAGTCATGGGCATGAGCGGTAGGTTAAAGCAAACCC
TGCAATATGGGCGGCGCTGATGTTGCTGCTGTGGGCGCAGTTCACGAAAT
GCTTGACCGCTTATGCAAGCAGATCTGCAAGCTCACAGCCCGACAGCTCG
TGGGCTCTGACTCCCATGAGCACAAGTGAGCAATAACGAAGCATGCCCTT
GCACGGCGTTGTCAACAGTACCTTGCGGTGGGGCATGCTATCTTGAGCAA
GTACTGTAATGTGGCGTTCGTGCCAATGCTCAGCATTGCTACATATACAC
AACACGCTCTTGGTCATTGCTTGGCAGTTCACGGGGCTATTGAGGTGGCG
GCTCATGAGCTGATGATCATCACTGCCAAACTGAAGTAAAATGCAATGCA
GACGCTTCGGTGGGGCTCAGTATCTATTCAACGATTGCAGAGTGGCGAAT
GATGGTGTTGGATCTCTTCTGCACATTCAAACATTCAAACAGTCATGAAC
AAAAATGAGTGGCAGCTCACTTGCAAACGGGTAACACATTGAGGCTGCCC
ATCCTCAAATAAGCCACAATCTGGCTTTTCTATCTCTAAGATGTGCTTGC
AGATGCACCTGTAGGCGGGCACCTGCAGAGATTGCAAGACACTAGTGCCG
ATCACTGAGCAGTCGCACCACGACGTGATGACTCTGCCTACACATGAAAA
CAAGTACACACATCTGCATGTATCCATCGACACCCCCCTCAATCACTGTC
ATTATTCGTTTGCTTACATGATGCCTCTGTGCTGATCTTGTGTGATTTGC
AGTCGGCGAACGAGCCATGATCACGGCCAGCCCTGACTATGCCTATGGTG
CAGGCGGGTTTCCTGCATGGGGAATCATGCCCAACAGTGTCCTCAAGTTT
GACATTGAGGTCCTCAGCATCAACTGA
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Coding sequence (CDS) from alignment at Contig_166:142893..144719-

>mRNA_13304 ID=mRNA_13304|Name=jgi.p|Trimin1|178167|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=339bp|location=Sequence derived from alignment at Contig_166:142893..144719- (Tribonema minus UTEX_B_3156 )
ATGGGCTTCGAGAAGGAGGTGCTTAAGGCGGGCAATGGACCCAAGCCGGC
CAGGGGACAATCGGTCACCGTTCACTGTACAGGATTTGGCAAGAATCAGG
ACCTCAACAGCAAATTCTGGAGCACTAAAGATCCCGGACAGCAGCCCTTC
ACATTCAACATAGGACTGGGCAGCGTCATCAAGGCGTGGGATGAAGGAGT
CATGGGCATGAGCGTCGGCGAACGAGCCATGATCACGGCCAGCCCTGACT
ATGCCTATGGTGCAGGCGGGTTTCCTGCATGGGGAATCATGCCCAACAGT
GTCCTCAAGTTTGACATTGAGGTCCTCAGCATCAACTGA
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