mRNA_2224 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|158720
Unique NamemRNA_2224
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A836CA22_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CA22_9STRA)

HSP 1 Score: 286 bits (732), Expect = 6.980e-98
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 1 
Query:    1 MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVSAAATYTDTLFYMLLKLVV 423
            MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVSAAATYTDTLFYMLLKLVV
Sbjct:    1 MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVSAAATYTDTLFYMLLKLVV 141
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A6H5L0T4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0T4_9PHAE)

HSP 1 Score: 214 bits (544), Expect = 1.310e-61
Identity = 99/123 (80.49%), Postives = 114/123 (92.68%), Query Frame = 1 
Query:    1 MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVS 369
            MG S+ +FELA+EARRA+ERFD+CEIKVI+ETFKDLCH++G + RIDKETFLQY PLPGLLGERLFAVFDKK+ GE+DYEEFVCGLAVTCRGSW EKVEFIFNLYD+HG GAV+R ELAAL++
Sbjct:    1 MGQSNSNFELASEARRAAERFDECEIKVIKETFKDLCHVNGGSMRIDKETFLQYLPLPGLLGERLFAVFDKKSSGEIDYEEFVCGLAVTCRGSWEEKVEFIFNLYDIHGQGAVNRDELAALLN 123
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A835YJT6_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJT6_9STRA)

HSP 1 Score: 173 bits (439), Expect = 1.170e-53
Identity = 81/104 (77.88%), Postives = 90/104 (86.54%), Query Frame = 1 
Query:   61 FDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVSA 372
            FD CEI V+R+TFKDLC +SG  T +DKETFLQYFPLPGLLGERLFAVFD K  G+VD+EEFVCGLAVTCRG W +KV+FIF+LYDVHG GAVSR EL ALVSA
Sbjct:    4 FDHCEITVLRQTFKDLCAISGKGTSVDKETFLQYFPLPGLLGERLFAVFDTKRSGKVDFEEFVCGLAVTCRGDWDQKVDFIFSLYDVHGTGAVSREELTALVSA 107
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: D8LIC5_ECTSI (N/a n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LIC5_ECTSI)

HSP 1 Score: 183 bits (465), Expect = 1.830e-51
Identity = 84/102 (82.35%), Postives = 95/102 (93.14%), Query Frame = 1 
Query:   64 DDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVS 369
            D+CEIKVI+ETFKDLCH++G + RIDKETFLQY PLPGLLGERLFAVFDKK+ GE+DYEEFVCGLAVTCRGSW EKVEFIFNLYD+HG GAV+R ELAAL++
Sbjct:   17 DECEIKVIKETFKDLCHVNGGSMRIDKETFLQYLPLPGLLGERLFAVFDKKSSGEIDYEEFVCGLAVTCRGSWEEKVEFIFNLYDIHGQGAVNRDELAALLN 118
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A7S3HBN9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HBN9_9STRA)

HSP 1 Score: 128 bits (321), Expect = 1.410e-31
Identity = 63/123 (51.22%), Postives = 86/123 (69.92%), Query Frame = 1 
Query:    1 MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALVS 369
            MGN+  S     EA+ A+++FD  EI V+R+T++DL   S D + IDKETFLQYFPL GLLGERLFA FD K  G +D+++F+ GLA  CRGS  +K+ F+FN+YDV     VS+ +L  L++
Sbjct:   14 MGNNSSSNNTHKEAKIAAQKFDTDEIHVLRKTWQDLSDRS-DGSGIDKETFLQYFPLNGLLGERLFAQFDNKKTGSIDFDDFILGLATVCRGSMDDKIHFVFNMYDVSHDNTVSKQDLTTLLN 135
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A2D4CGZ4_PYTIN (Protein kinase, putative n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CGZ4_PYTIN)

HSP 1 Score: 118 bits (295), Expect = 1.380e-28
Identity = 64/127 (50.39%), Postives = 84/127 (66.14%), Query Frame = 1 
Query:    1 MGNSHGSFELAA-----EARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALV 366
            MGNS  + E +A     E R A ++F + E+ ++RETFK L      NT +DKETFL+ FPLPGLLGERLF V DK   G + Y EFV GLA+  RGS  EK++FIF+LYD+   G++SR EL  ++
Sbjct:    1 MGNSPLTREFSARRLSVEEREAMKKFCEEEMHLLRETFKGLADAKNGNT-VDKETFLKCFPLPGLLGERLFEVIDKDGSGSIAYNEFVYGLAILFRGSQREKLKFIFDLYDLTATGSISRHELVTML 126
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A6G0WE42_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WE42_9STRA)

HSP 1 Score: 118 bits (295), Expect = 4.050e-28
Identity = 58/125 (46.40%), Postives = 83/125 (66.40%), Query Frame = 1 
Query:    1 MGNSH---GSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALV 366
            MGNS    G F L  E + A +RF+D EI ++RE F+ +   +GD   +DKE FL+ FP+PGLLGERLF +FDK     + + EFV GLA+  +GS  EK++FIF+LYDV   G++S+ E+  ++
Sbjct:    1 MGNSASGGGVFALTGEEKEALKRFEDAEIHLLREVFRGITQATGDKA-VDKENFLKIFPMPGLLGERLFTLFDKNASNSISFNEFVGGLAILTKGSRNEKIKFIFDLYDVSDKGSISKREMKTML 124
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A024TDC6_9STRA (CAMK/CAMK1 protein kinase, variant 2 n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TDC6_9STRA)

HSP 1 Score: 117 bits (293), Expect = 4.660e-28
Identity = 56/121 (46.28%), Postives = 81/121 (66.94%), Query Frame = 1 
Query:    4 GNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALV 366
            G   G   L+ E + A  RF+D EI ++RE FK +   + DN+ +DKE FL+ FP+PGLLGERLFAVFDK     + + EF+ GLA+  +GS  EK++FIF+LYDV   G++S+ E+  ++
Sbjct:    7 GRMDGIHGLSGEEKDAIRRFEDAEIHLLREVFKGITQTTADNS-VDKENFLKIFPMPGLLGERLFAVFDKNASNSITFNEFIGGLAILTKGSRNEKMKFIFDLYDVSDKGSISKREMTTMI 126
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: A0A024TDM7_9STRA (CAMK/CAMK1 protein kinase n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TDM7_9STRA)

HSP 1 Score: 117 bits (293), Expect = 7.690e-28
Identity = 56/121 (46.28%), Postives = 81/121 (66.94%), Query Frame = 1 
Query:    4 GNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALV 366
            G   G   L+ E + A  RF+D EI ++RE FK +   + DN+ +DKE FL+ FP+PGLLGERLFAVFDK     + + EF+ GLA+  +GS  EK++FIF+LYDV   G++S+ E+  ++
Sbjct:    7 GRMDGIHGLSGEEKDAIRRFEDAEIHLLREVFKGITQTTADNS-VDKENFLKIFPMPGLLGERLFAVFDKNASNSITFNEFIGGLAILTKGSRNEKMKFIFDLYDVSDKGSISKREMTTMI 126
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BLAST of jgi.p|Trimin1|158720 vs. uniprot
Match: W4G4J0_9STRA (CAMK/CAMK1 protein kinase n=6 Tax=Aphanomyces astaci TaxID=112090 RepID=W4G4J0_9STRA)

HSP 1 Score: 117 bits (293), Expect = 7.710e-28
Identity = 55/121 (45.45%), Postives = 82/121 (67.77%), Query Frame = 1 
Query:    4 GNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKETFLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEFIFNLYDVHGVGAVSRGELAALV 366
            G   G + L+ + + A  RF+D EI ++RE FK +   + DN+ +DKE FL+ FP+PGLLGERLFAVFDK     + + EF+ GLA+  +GS  EK++FIF+LYDV   G++S+ E+  ++
Sbjct:    7 GRMDGVYGLSGDEKEAILRFEDAEIHLLREVFKGITQTTADNS-VDKENFLKIFPMPGLLGERLFAVFDKNASNSITFNEFIGGLAILTKGSRNEKMKFIFDLYDVSDKGSISKREMTTMI 126
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|158720 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CA22_9STRA6.980e-98100.00Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A6H5L0T4_9PHAE1.310e-6180.49Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835YJT6_9STRA1.170e-5377.88Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
D8LIC5_ECTSI1.830e-5182.35N/a n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepI... [more]
A0A7S3HBN9_9STRA1.410e-3151.22Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A2D4CGZ4_PYTIN1.380e-2850.39Protein kinase, putative n=1 Tax=Pythium insidiosu... [more]
A0A6G0WE42_9STRA4.050e-2846.40Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A024TDC6_9STRA4.660e-2846.28CAMK/CAMK1 protein kinase, variant 2 n=1 Tax=Aphan... [more]
A0A024TDM7_9STRA7.690e-2846.28CAMK/CAMK1 protein kinase n=2 Tax=Aphanomyces inva... [more]
W4G4J0_9STRA7.710e-2845.45CAMK/CAMK1 protein kinase n=6 Tax=Aphanomyces asta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_6contigContig_6:1628682..1630870 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|158720|e_gw1.6.78.1
BRITEko00000,ko00001,ko01000,ko01001
KEGG Pathwayko04921,ko04925,map04921,map04925
KEGG koko:K08794,ko:K08808
EC2.7.11.1,2.7.11.17
Descriptionprotein serine/threonine kinase activity
COG categoryT
Max annot lvl2759|Eukaryota
EggNOG OGsKOG0032@1|root,KOG0032@2759|Eukaryota
Evalue3e-52
Seed ortholog2880.D8LIC5
Hectar predicted targeting categoryother localisation
TranscriptId159042
TrackFilteredModels1
ProteinId158720
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|158720gene_2224Tribonema minus UTEX_B_3156 geneContig_6 1628682..1630870 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_2224_1exon_2224_1Tribonema minus UTEX_B_3156 exonContig_6 1628682..1628828 +
exon_2224_2exon_2224_2Tribonema minus UTEX_B_3156 exonContig_6 1630592..1630870 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_2224CDS_2224Tribonema minus UTEX_B_3156 CDSContig_6 1628682..1628828 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|158720Trimin1|158720|e_gw1.6.78.1Tribonema minus UTEX_B_3156 polypeptideContig_6 1628682..1630870 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_2224 ID=mRNA_2224|Name=jgi.p|Trimin1|158720|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=426bp|location=Sequence derived from alignment at Contig_6:1628682..1630870+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGGGCAATTCGCATGGATCCTTCGAGCTGGCGGCGGAGGCGAGGAGGGC
TTCAGAGAGGTTTGACGATTGCGAGATCAAAGTGATCAGGGAGACTTTCA
AGGATCTCTGCCACCTGAGCGGCGACAACACTCGCATAGACAAAGAGACC
TTTCTGCAGTACTTCCCGCTGCCGGGGCTGCTCGGCGAGCGCCTGTTTGC
GGTCTTCGACAAAAAGAACATTGGCGAGGTGGACTATGAGGAGTTTGTGT
GCGGGCTGGCGGTGACGTGCCGCGGAAGTTGGGCGGAGAAGGTGGAGTTC
ATCTTCAACCTGTACGACGTGCACGGGGTCGGGGCGGTGTCGCGCGGGGA
GCTGGCGGCGCTGGTGAGCGCTGCTGCAACGTATACAGACACATTGTTTT
ATATGTTGCTGAAGCTAGTTGTTTAG
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protein sequence of jgi.p|Trimin1|158720

>Trimin1|158720|e_gw1.6.78.1 ID=Trimin1|158720|e_gw1.6.78.1|Name=jgi.p|Trimin1|158720|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=142bp
MGNSHGSFELAAEARRASERFDDCEIKVIRETFKDLCHLSGDNTRIDKET
FLQYFPLPGLLGERLFAVFDKKNIGEVDYEEFVCGLAVTCRGSWAEKVEF
IFNLYDVHGVGAVSRGELAALVSAAATYTDTLFYMLLKLVV*
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mRNA from alignment at Contig_6:1628682..1630870+

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_2224 ID=mRNA_2224|Name=jgi.p|Trimin1|158720|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=2189bp|location=Sequence derived from alignment at Contig_6:1628682..1630870+ (Tribonema minus UTEX_B_3156 )
ATGGGCAATTCGCATGGATCCTTCGAGCTGGCGGCGGAGGCGAGGAGGGC
TTCAGAGAGGTTTGACGATTGCGAGATCAAAGTGATCAGGGAGACTTTCA
AGGATCTCTGCCACCTGAGCGGCGACAACACTCGCATAGACAAAGAGGTG
CGTGGCTTCCATCCGCCCTCACCTCAATTACTTTATGCATATGTTGCCAC
GCTGGCTACGCACGCGTGCGTGTGCTGTGCTCTGTCCTTCTGTGGGAAGT
TGGTTTCCCGTCTGTTTGCGGTGCGGAGGTGCTGATGTGATCATGCAGAA
TCAGCCCAACTCAGCAAGAAAGCCCGGTAACACTACACCTTCTTTCAACC
ATCTGTTACTGTAATACTAGTGGTACACGAAGTATCATTTTCATGAGTGT
GGTGTCTGCCAGAAATGACAATTTCACAACAGCTATGCATGCCATTTCTG
CAGCAGCAAGTTTGCCTGAGCGCACTTGCAGCGCTCGTGCGCCGCAGCAT
ATGGCACGCCTGCATCGCACGACGTCGGCTAGCGTGGGCTCTGGACTCAG
AGCGTACCTCAACATCTGAGGTACTGAACTGCTTCGTGTAGAAGTACACA
CATCATCTGGGCTTTTTCGCGCCCTGAGCGAGAATTAATAAAAAGTACAC
GCACCAGTTTTGTGTTGTCCCCAGTATTTGGTGCGCCATATATCCCGTGC
CCCCCCGTGGAGCCCCAATTATGCATGACGCTGGTGCAGCATTGTCTCTG
CGACACGCGCTCTAACGCTACTCACTGCTGCGGATATAGACTTTCCACAT
GGTCAGTCAGTCAATACAAGCCTGCCAGTCAGTAGGGTCAGTGCTCAGTT
TTGCGCCTGACATGCCTCAGCGTGCGGCCTCTGTGCGAAACTGCCGTGAG
CTGCGCGCCGCCAGCGCCCTTATGCGGCGGCGCCGCGGCGCTGTGCACAG
CCTCCGCAGGCGCTGCGTTAAACTTCAAGAGCGCGTGCGCGGCGTGCCCA
GCGTCGGCGGTTAGCGAAGTTGGCGGTGAGCGCACCATGCCAATGTCTAA
GAAAGGCACTTACTTTTGAAATCTTGCTAACTGCACCGGGACGTCACCAT
AGAGAAGCATTGCTCAGCTCTCACCATAGAGAAGCATTGCTCCGCTCTGA
GGCGCTGAGCGCAATGCATTTGCACACGATGGCTTTCCTCGTTTTTTATA
ATGGCACTTTTCGTCTAACCTATCTGCAACGGCTTGATGACGGCGATGCG
TCACCGCACCGCCGGAAGAGCCGCCACGGCGCAGCACATCTCTCGGCGTG
TGGATTCTTTCATGGGGTGCGGCGCCGTGCGGACAGCGCCATCTGCGACA
GCCGATGGAAACGGCTGTTACGTCAGTGCTCAGCAGGGGTCGATCTACAT
TAACAAGTAATACATACACACTCAGTTTGGAATTCAAGCAATTGCCTCGC
GGCTGCGAGGCGCGGGTTTTCACAGTTTGCAACGCCGTGCGCAGCACCGT
TGCGCAGCACCGTCGGCGACACCATGAGGCAGCGCGTGAAACTTCAATGT
GAGGTCAAAGAGAGAAACGGACACGGCAGTCGTGCTCAGCAGCGGCTGGC
TGCGCCGCGGTCTCCGCTGCTGCGGAGCGCTGCTGCGGAGCGCGGCGTGC
CGCCGCCGCCGCTGCTTTGGTCTGCCGACTGCGTGCACGGCGCGCTGCAC
GTTGCTGGCCCCCATTCATTTGACTGCGTTACGCCAACTCTCCTGCAGGC
ATGGCATTTACGCCCCCCCCCCCTGGCAGACACATGACACTGAGCCGTCG
GCGCTATTGCGTGAGGCGTAACACAAAGCCACGTGCCTCCAGGCAACTAT
ATCATGCCGCGACCAATCCCCCTGCACCCCCCCCTCCCTCCTCCCCCCTG
AGCGCTGCAGACCTTTCTGCAGTACTTCCCGCTGCCGGGGCTGCTCGGCG
AGCGCCTGTTTGCGGTCTTCGACAAAAAGAACATTGGCGAGGTGGACTAT
GAGGAGTTTGTGTGCGGGCTGGCGGTGACGTGCCGCGGAAGTTGGGCGGA
GAAGGTGGAGTTCATCTTCAACCTGTACGACGTGCACGGGGTCGGGGCGG
TGTCGCGCGGGGAGCTGGCGGCGCTGGTGAGCGCTGCTGCAACGTATACA
GACACATTGTTTTATATGTTGCTGAAGCTAGTTGTTTAG
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Coding sequence (CDS) from alignment at Contig_6:1628682..1630870+

>mRNA_2224 ID=mRNA_2224|Name=jgi.p|Trimin1|158720|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=426bp|location=Sequence derived from alignment at Contig_6:1628682..1630870+ (Tribonema minus UTEX_B_3156 )
ATGGGCAATTCGCATGGATCCTTCGAGCTGGCGGCGGAGGCGAGGAGGGC
TTCAGAGAGGTTTGACGATTGCGAGATCAAAGTGATCAGGGAGACTTTCA
AGGATCTCTGCCACCTGAGCGGCGACAACACTCGCATAGACAAAGAGACC
TTTCTGCAGTACTTCCCGCTGCCGGGGCTGCTCGGCGAGCGCCTGTTTGC
GGTCTTCGACAAAAAGAACATTGGCGAGGTGGACTATGAGGAGTTTGTGT
GCGGGCTGGCGGTGACGTGCCGCGGAAGTTGGGCGGAGAAGGTGGAGTTC
ATCTTCAACCTGTACGACGTGCACGGGGTCGGGGCGGTGTCGCGCGGGGA
GCTGGCGGCGCTGGTGAGCGCTGCTGCAACGTATACAGACACATTGTTTT
ATATGTTGCTGAAGCTAGTTGTTTAG
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