mRNA_18575 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|50087
Unique NamemRNA_18575
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: A0A835Z0R8_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z0R8_9STRA)

HSP 1 Score: 228 bits (581), Expect = 1.150e-74
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 1 
Query:   70 MLAEAEAMAPLVDKENWDGVLAKTRGAPLTLLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQALAALLE 453
            MLAEAEAMAPLVDKENWDGVLAKTRGAPLTLLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQALAALLE
Sbjct:    1 MLAEAEAMAPLVDKENWDGVLAKTRGAPLTLLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQALAALLE 128
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: W7TZC3_9STRA (Uncharacterized protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TZC3_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 5.840e-18
Identity = 58/139 (41.73%), Postives = 80/139 (57.55%), Query Frame = 1 
Query:   34 SSRADFDAALKGMLAEAEAMAPLVDKENWDGVLAKTRGAPLTLLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQALAALL 450
            +S +D +  ++ M     A++PL+ KE WD VLA  +  PL LL +  LG G+ + LA+ +   L K+  + E   EA VALQQL DYAFSNRV+FFN +DK QV  L+       +L EP  LL D    ++AL   L
Sbjct:  134 ASPSDPEELIESMTDTLYALSPLIKKEGWDDVLAAVKRKPLCLLLTPFLGYGNAAELAKALGGDLKKSRALEERRQEASVALQQLVDYAFSNRVIFFNSLDKQQVTALAS-GRAPPDLSEPLGLLEDTVGLVKALQETL 271
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: A0A7S0E077_9CRYP (Hypothetical protein n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0E077_9CRYP)

HSP 1 Score: 56.2 bits (134), Expect = 2.010e-6
Identity = 40/118 (33.90%), Postives = 63/118 (53.39%), Query Frame = 1 
Query:  118 WDGVLAKTRGAPLT-----------LLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQAL 438
            WD + A+   APL            +L S   G  S   L +T+++    A  + +   EA + L +L ++AF+NRVVFFN  DK  V +LSE+ N + +LDE   L+ D K++I+ +
Sbjct:  129 WDDIRAELLTAPLANQKFSKPDRPAILFSTFFGFKSEKELMKTLNIDKQAANRIEKLRKEASLTLMELAEFAFTNRVVFFNEEDKKTVAELSEK-NLQVDLDEALRLVQDAKERIREI 245
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: A0A813HS02_POLGL (Hypothetical protein (Fragment) n=1 Tax=Polarella glacialis TaxID=89957 RepID=A0A813HS02_POLGL)

HSP 1 Score: 49.7 bits (117), Expect = 5.420e-5
Identity = 29/67 (43.28%), Postives = 41/67 (61.19%), Query Frame = 1 
Query:  253 AAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQ---LSEETNYKAELDEPRELLADVKQQIQALAA 444
            A ++ G+ALQQLE++ FSNR++ FN  DK  V++   L      K EL EP E L D ++ +Q L A
Sbjct:   27 ALSDVGLALQQLEEWCFSNRIIIFNKDDKTIVERDKVLGAANAKKVELAEPLEYLKDARENLQKLRA 93
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: A0A7S3XUV8_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XUV8_HETAK)

HSP 1 Score: 52.0 bits (123), Expect = 6.470e-5
Identity = 25/56 (44.64%), Postives = 39/56 (69.64%), Query Frame = 1 
Query:  271 VALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQAL 438
            V L+QL+DYAFSNRV+FFN+ DK  V ++SE+ +   +L EP  +L + +  I+ +
Sbjct:  192 VLLKQLDDYAFSNRVLFFNLEDKKAVTEMSEQNDTSLDLSEPLAILDECRDLIKQI 247
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BLAST of jgi.p|Trimin1|50087 vs. uniprot
Match: L1I603_GUITC (Uncharacterized protein n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1I603_GUITC)

HSP 1 Score: 51.6 bits (122), Expect = 9.280e-5
Identity = 38/118 (32.20%), Postives = 60/118 (50.85%), Query Frame = 1 
Query:  118 WDGVLAKTRGAPLT-----------LLKSAALGLGSVSALARTVSLSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEETNYKAELDEPRELLADVKQQIQAL 438
            WD + A+   APL            +L  +  G  S   L   + +    A  + +   +A + L +L ++AF+NRVVFFN  DK  V +LSE+ N + +LDE   L+ D K++IQ +
Sbjct:  140 WDDIRAEMLTAPLANQKFSKPDRPAILFLSFFGFKSEKELMTVMKIDKKAATRIEKLRKDASLTLMELAEFAFTNRVVFFNEEDKRTVAELSEK-NLQVDLDEALRLIQDSKERIQEI 256
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|50087 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
A0A835Z0R8_9STRA1.150e-74100.00Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
W7TZC3_9STRA5.840e-1841.73Uncharacterized protein n=2 Tax=Monodopsidaceae Ta... [more]
A0A7S0E077_9CRYP2.010e-633.90Hypothetical protein n=1 Tax=Hanusia phi TaxID=303... [more]
A0A813HS02_POLGL5.420e-543.28Hypothetical protein (Fragment) n=1 Tax=Polarella ... [more]
A0A7S3XUV8_HETAK6.470e-544.64Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
L1I603_GUITC9.280e-532.20Uncharacterized protein n=1 Tax=Guillardia theta (... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_256contigContig_256:98489..103240 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|50087|CE50086_27
Max annot lvl2759|Eukaryota
EggNOG OGs2EYYH@1|root,2T0D1@2759|Eukaryota
Evalue5.19e-16
Seed ortholog2880.D7FWN3
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionexpressed unknown protein
Ec32 orthologEc-06_001300.1
TranscriptId50409
TrackFilteredModels1
ProteinId50087
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|50087gene_18575Tribonema minus UTEX_B_3156 geneContig_256 98489..103240 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|50087Trimin1|50087|CE50086_27Tribonema minus UTEX_B_3156 polypeptideContig_256 98525..102501 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_18575_6exon_18575_6Tribonema minus UTEX_B_3156 exonContig_256 98489..98635 -
exon_18575_5exon_18575_5Tribonema minus UTEX_B_3156 exonContig_256 99379..99412 -
exon_18575_4exon_18575_4Tribonema minus UTEX_B_3156 exonContig_256 100263..100309 -
exon_18575_3exon_18575_3Tribonema minus UTEX_B_3156 exonContig_256 101148..101213 -
exon_18575_2exon_18575_2Tribonema minus UTEX_B_3156 exonContig_256 102373..102543 -
exon_18575_1exon_18575_1Tribonema minus UTEX_B_3156 exonContig_256 103214..103240 -


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
UTR3_18575UTR3_18575Tribonema minus UTEX_B_3156 three_prime_UTRContig_256 98489..98524 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_18575CDS_18575Tribonema minus UTEX_B_3156 CDSContig_256 102373..102501 -


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
UTR5_18575UTR5_18575Tribonema minus UTEX_B_3156 five_prime_UTRContig_256 103214..103240 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_18575 ID=mRNA_18575|Name=jgi.p|Trimin1|50087|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=492bp|location=Sequence derived from alignment at Contig_256:98489..103240- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
GATGGGGGCACCAAGGTCCAGCAACTGGTGCTCAGCAGCAGGGCGGATTT
CGACGCGGCGCTGAAAGGCATGCTGGCAGAGGCTGAGGCCATGGCGCCGC
TCGTGGATAAGGAGAACTGGGATGGAGTGCTGGCAAAGACCAGGGGCGCG
CCGCTGACGCTGCTCAAGAGCGCGGCGCTGGGCCTGGGCTCCGTTTCGGC
ACTGGCACGCACTGTTTCTCTGAGTCTCGCGAAGGCCGCAGAAGTGTCTG
AGGCAGCAGCAGAGGCAGGAGTTGCGCTGCAGCAGCTTGAAGACTATGCC
TTCTCCAACAGGGTTGTGTTCTTCAACATGGTCGACAAAAACCAGGTCCA
GCAGCTGAGCGAGGAGACCAACTACAAGGCCGAACTGGACGAACCCCGGG
AGCTTCTTGCTGATGTGAAGCAGCAGATACAGGCTCTGGCAGCTCTGCTA
GAGTAGAATGGCACGTTTTAGAACCTGAACAGACCAAATCGG
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protein sequence of jgi.p|Trimin1|50087

>Trimin1|50087|CE50086_27 ID=Trimin1|50087|CE50086_27|Name=jgi.p|Trimin1|50087|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=129bp
MLAEAEAMAPLVDKENWDGVLAKTRGAPLTLLKSAALGLGSVSALARTVS
LSLAKAAEVSEAAAEAGVALQQLEDYAFSNRVVFFNMVDKNQVQQLSEET
NYKAELDEPRELLADVKQQIQALAALLE*
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mRNA from alignment at Contig_256:98489..103240-

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_18575 ID=mRNA_18575|Name=jgi.p|Trimin1|50087|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=4752bp|location=Sequence derived from alignment at Contig_256:98489..103240- (Tribonema minus UTEX_B_3156 )
GATGGGGGCACCAAGGTCCAGCAACTGGTGCGTCTGAGCAGCATGAGCAG
CACCTCCTGATTGTAGTTCGTGGTGATAATTGCTTCTCTGCCTGCTGCAC
AAATAGTTTGAACCGCTTTTGTTGGCATAAGCTATGAGCAAGCTCGAATC
CATGTCTTCCTTGCAATTGCTGTACATGGGTGCCGTTCGTATCGTCAACA
CCATCCGGTGATGCGTATGTCCGCTTGTGCGAGACCTGCATTCAGTGCAG
CTGCTCAACAGTCACGCCGACACTCGCGTCAACATCTCCGGAGGATGTTG
CTCACTGTGGTTGGGTTGAACCTGCTGTGTGTGCATCGTGTGCTTAGCCA
TCGTGTGCTTAGCCGCTACATCAACAACAGCTGTGACAGCAGTGACGACT
GCGAGCACGCGCTCGCATGTCGGGCGATCACAGCGGCTCCGCCAGCGTGC
AGATGCTGATGCAGTATCAGCGAACACCGTCAGCCAACACGCCGTCACCC
CTGTCATTTAAGTTGCCCTCTGTGCGCCCGCCTCTCCACACAAACGTTGA
TGCAATAGCAGATGCTCAGCAGCCCAGGGACCCCCCGGCTTCTGCTCCAC
CAACACCGGCACGCCCTCGGTGTGCACTGTCGCCGTGTCAGCTGGCGCTC
ATGCGAGGTCTGCCCATGCGCCCCCTTGGCTTCGTGCGCGATCGCAGGTG
CTCAGCAGCAGGGCGGATTTCGACGCGGCGCTGAAAGGCATGCTGGCAGA
GGCTGAGGCCATGGCGCCGCTCGTGGATAAGGAGAACTGGGATGGAGTGC
TGGCAAAGACCAGGGGCGCGCCGCTGACGCTGCTCAAGAGCGCGGCGCTG
GGCCTGGGCTCCGTTTCGGTGAGCGCGCGTTGTGCTGATGTTGAAGTTGA
AGTGCATGCAGGCTGAGAAGGCTCAGAGATTGGGCATGTCGGAAAATAAG
GGCGCGTACAGCTGAGCTGCACGTTCGATGAGCATTTGGTATGATGACAT
TGATGCAACTGCATGTATGTACACAACACTCAACCCTGAGCGCGGCGCTG
GGCCTGGGCTGCGCGTTTCGGTGAGCGCGCGTTGTGCTGGTGTTGAAGTT
GTGAAGTGCGTGCACACTGAGTCAAGCCCAGAATAAGCAAGTTGAAATGC
GTGCACACTGATGAGAGAAGGGGCCCAGAATGAGCATGTCAGAAATTGGG
CGCATTCAGCTGAGCTCCACGTCTGATGAGCATGCGGCTTCGTGACACTG
ATGTGACTGAATGTATGTCTTTCTAAATACTCAACCCTGAACGTGGATCG
GTTAGTCAGGGTTGCGTGGTTTCAGTCGAAATGCAGCTCTTCTGCGATAC
TGCTGGAGAGTGGGCTAAAGTGATTGCCCAAACATTCAAACTCGTCGCCG
TGTTCGGGCTCTCCCCCATCCGCACCAACACAAGTGTTGTCCGTGACAAT
TTTTGAAGCACCTACCTTTGAGCGCATACAAAACGCCCCAGTTCAATCTT
ACTTCTGTGCACACTTCGCGACAAGCCATGACTCATGGCACCAGCGTGTA
GGCTCACAAGTTTGACCAACGCGGAGTTTGTGATCTGCACATGCGCATGG
AAAAAAAGGGGCTGTGGAAGCGCTGTGGAAGCACGAGTGCTACGTGTAGT
TGGCCTCATTGCACAAATTGGATTACTGTAGCAGGCTCGTCTGTCATGAT
CAATGAGTGGAGGCCAAATCGTTGTCAACAGCGCATTGAGCGCTGTCACC
ATTCTCTTTGTCTCATCACCAGCTGTCCAAGCACTGCCGTACCATACGTA
CTGTACATACCTGCGCCTGGTGCACATTACAACGTGGTTGTTTCGCGGGC
CGAATACATATGTGATAAGTACTTTGAAAGCCTGCGCTGCAATCGAGAGA
GTGGCTGCCACAAACCTGCTGAGCACTGCTGCGGTCATCTGCGCACCGCA
TGTGCTCTGAGACAACTGAACAACTGCTCTAGGATACCGATACCGCATCG
TATGCTTGCCGCGTTTGACATGTGCAGGCACTGGCACGCACTGTTTCTCT
GAGTCTCGCGAAGGCCGCAGAAGTGTCTGAGGCAGCAGCAGAGGTGAGCG
CTGCTTGAGAAGCTGCTTTTTGAAGTTGAGCTTCAAGTGCAGCAGCGCAT
GTGGTACAAACCTGCAGCTTGGAGTGTTTCTGCCTGCTTGAAGTGCGGTT
GACGCCCCGCACCACGATGCTGATTCTGTCAAAATGCAGAATCACAAACA
GCGCTGTGTCGATGGCGACGCGATAAAGCTCATGCAGGGCCTGGGTTGCG
GCCTGGGTTGCGTGCAGCAGAAATGCCTGCTGCTGTGCGCATGCGACTTG
ACATGAAATGCTGCCACTCCATTCATGCACCAGCTGCCAGCACGCCGATA
CCTGGCCCTAGCTCAGGATACAGACGCCATCGTGCAGCAATTGCTATACA
AGTACAGCTCTCTGCGTGATGATTTGATTGGTCAACTCAGGCTATGGTCA
GAGTAAGGGTTACATCGAGGCTTTACATTACCACCCCAAAATGCTGCCAT
GCTCATGTTGCTCCAGCCACGTACACTATTCTTCTTATTCTTGGGAGGTG
GCATTTCCAGATGCGCACTTTGGACTCCAAGGCTGTGGTTACAAGCTCGT
GAATGCCATTTGAGGTCGCGTGAAAACTGAATGCCCGCAGCGGTTTTTGT
ACCACCAACAGCTGTGCAGTTGCGGTGTTGTGTGGAATCATTGTCAATCT
CTGTACTTCACCGATGAAGGTTGTGAATACAGGTTCACAGCGTGTCACGG
TTCGCAGCCATTGGTGGTGGGGTGCTGCGGCAGAATTTCAACTCTTGCGG
GCGTGCATCTGTAGTCTATTCATGCGGCTCACAGGCGGTTTCACTTCAGC
GTTGACACTGCGTGTCGCATTCGCGCTGCAGGCAGGAGTTGCGCTGCAGC
AGCTTGAAGACTATGCCTTCTCCAACAGGCAAGTTCGTGAGCCCCCAAAA
CCTCAAGTGCAACCGCAGCTGTGTGCCAGCGCCCCATTGGGGCTTTCATT
GAAATACCTGGAAGTATGGGGTTATGGTTTGCCAAAGATGCGCACGCAAT
ACTTGCAACTCTTTGCATACCACTGGTCTATGCTCTGCACAATGATGAAG
CCGACATGCGCGGGGGTCAGATCACATTCGCAAACGCGAGCAACTTGGGG
CAAGAGGTTGGAGAGTTTTTGTATACTTTGCGTATGTACCTGAAGTTTGG
ATCACGTTGTGATCATTGTCAGCACCACTGGTAGGAGACTGGTTTGACTG
CTCACGGTTGTGATCACGGTTGTGAGAGGGCCTTTCACTTGCTGCTTGTT
GGCGAGTTTGCTGCATTCCTTGATGCGCATGTCGCTGGTGCAGACCGCCA
ACCAGGCGCGGCAGTTTTTACCCTAACCCTAACCCTCACCTTTGTGATCC
CAACGCCTATACCCAATTTTTAACCCTACAATCTGACCTCAAGCCTACAC
CTAGTCCTAGCCCTAGCCCTGAGGAGCGCGAGCGAACGGCCATTGTGCGT
AGCGCTGCCGTCACAAGTTGTGCAGCGCGACCCTTGCGGCGACACCAACT
GCCACACAGTGACGCATTGCATGTTTGTGGCGCCGTCAAGAACGCCGCCA
GCCTTGCGGCTCGAAGACCTTGCTGTGCGACGATAAGCACTGCATTTATC
ACATGAGATAAAAGTATGATTTATCTGCGGGGTATTTCACAAGGTATGTA
CTTCTATGTATGTGGGCGCCAGTCAGCCACCTTGCGTTTGACCTCTGTTC
TGCCTGCACGCACCCTCATTCGTGACAGGGTTGTGTTCTTCAACATGGTC
GACAAAAACCAGGTGGGCGGTGCTGTCACTCATGTGCCTGCTTGGCCCAC
CATTCTCGACACTCACAAAGCCAGACAAAGCAACGTGATACAGTACAATG
AGGCACACGTTGAGATTCAGGCTATAACCTGCAAAGTACTGATGAAAGAG
TGGCTCAGGTGCTGAGTTGTGCTTTGTTCAGGTGCACACGGCGCACTTCA
AGTAAATTGTGTATGCTGGTGCCTCCTGGGCCCAATTTTGCAGCAGCACA
TGATGTTACATTTGAGGAGTGCACTGCCCGGCGCTTTGAAGCACGTGCCA
GATGTACCGTGGTAGCGCCCACAATGCTGCCATTCATGATGGTAAGCCCT
GCGCTGAGTGACTTGCAAAGCTGTTGCCATGCTAGTGACGCTTGATGTAC
CAGCATCTGTGAGCTGGGAACCTTTAAGATCAGGGTTGGAAACCCTGAAG
GGAAAACTGTGACTGGAAGGATTTGACCATATTTTGTGCCTGATCCTCCT
GCATTTTTTTGCGGCTATGAGGATCGCAGACCTACTTACATGTCTAAGGC
TAGTCCATTCAGTGTTGGCAGTGAAATGTGGAGTGTGCACGCATGACAGC
GCAAATGCGCCCTGCCCGCGTGTACTGACTATGGCAACTTCATTTTTGTA
CTGTAGTGTACCGACTACTTGTGGTGGTTCACGTTTGTACTGTAGTGTAC
CGACTATGGTAGTTCACATGTGTAATGTACTGTACTTGTTTTTTTTTTAC
TGCAGGTCCAGCAGCTGAGCGAGGAGACCAACTACAAGGCCGAACTGGAC
GAACCCCGGGAGCTTCTTGCTGATGTGAAGCAGCAGATACAGGCTCTGGC
AGCTCTGCTAGAGTAGAATGGCACGTTTTAGAACCTGAACAGACCAAATC
GG
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Coding sequence (CDS) from alignment at Contig_256:98489..103240-

>mRNA_18575 ID=mRNA_18575|Name=jgi.p|Trimin1|50087|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=387bp|location=Sequence derived from alignment at Contig_256:98489..103240- (Tribonema minus UTEX_B_3156 )
ATGCTGGCAGAGGCTGAGGCCATGGCGCCGCTCGTGGATAAGGAGAACTG
GGATGGAGTGCTGGCAAAGACCAGGGGCGCGCCGCTGACGCTGCTCAAGA
GCGCGGCGCTGGGCCTGGGCTCCGTTTCGGCACTGGCACGCACTGTTTCT
CTGAGTCTCGCGAAGGCCGCAGAAGTGTCTGAGGCAGCAGCAGAGGCAGG
AGTTGCGCTGCAGCAGCTTGAAGACTATGCCTTCTCCAACAGGGTTGTGT
TCTTCAACATGGTCGACAAAAACCAGGTCCAGCAGCTGAGCGAGGAGACC
AACTACAAGGCCGAACTGGACGAACCCCGGGAGCTTCTTGCTGATGTGAA
GCAGCAGATACAGGCTCTGGCAGCTCTGCTAGAGTAG
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