Trimin1|349462|estExt_Genemark1.C_Ctg_430060 (polypeptide) Tribonema minus UTEX_B_3156

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A836CCI7_9STRA (Subtilisin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CCI7_9STRA)

HSP 1 Score: 1649 bits (4269), Expect = 0.000e+0
Identity = 940/940 (100.00%), Postives = 940/940 (100.00%), Query Frame = 0

Query:    1 MAVDRVHAHNRRVLPSADGAGDSYTGQDGTARVHLSPLPHLAKLSGDLALALQPDPSDPLVAAHSGRRLQEGVPLPRRLLSKVSASTAVGVEVILAPGAAGDERGARALAERWLAELADTPTXXXXXXXXXXXXXXXXXXXXXAAAAHMRRWHFWAGEHRHARRLDAMGGHAVAQRGGWRALMTQFDKLGMAHVAVYVRNASAAXXXXXXXXXXXXXXXXXDACDFLSVAVDASPRGHKFRLDLMELAKRDADGTCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFIXXXXXXXXXXADGGANDNLSNLFTETTGIKMTWQPRKGAAAAKASADAAEKCPECGVECSDVVKLVEHVQRKQQAQQDCC 940
            MAVDRVHAHNRRVLPSADGAGDSYTGQDGTARVHLSPLPHLAKLSGDLALALQPDPSDPLVAAHSGRRLQEGVPLPRRLLSKVSASTAVGVEVILAPGAAGDERGARALAERWLAELADTPTXXXXXXXXXXXXXXXXXXXXXAAAAHMRRWHFWAGEHRHARRLDAMGGHAVAQRGGWRALMTQFDKLGMAHVAVYVRNASAAXXXXXXXXXXXXXXXXXDACDFLSVAVDASPRGHKFRLDLMELAKRDADGTCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFIXXXXXXXXXXADGGANDNLSNLFTETTGIKMTWQPRKGAAAAKASADAAEKCPECGVECSDVVKLVEHVQRKQQAQQDCC
Sbjct:    1 MAVDRVHAHNRRVLPSADGAGDSYTGQDGTARVHLSPLPHLAKLSGDLALALQPDPSDPLVAAHSGRRLQEGVPLPRRLLSKVSASTAVGVEVILAPGAAGDERGARALAERWLAELADTPTXXXXXXXXXXXXXXXXXXXXXAAAAHMRRWHFWAGEHRHARRLDAMGGHAVAQRGGWRALMTQFDKLGMAHVAVYVRNASAAXXXXXXXXXXXXXXXXXDACDFLSVAVDASPRGHKFRLDLMELAKRDADGTCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFIXXXXXXXXXXADGGANDNLSNLFTETTGIKMTWQPRKGAAAAKASADAAEKCPECGVECSDVVKLVEHVQRKQQAQQDCC 940          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: D7FS20_ECTSI (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FS20_ECTSI)

HSP 1 Score: 427 bits (1098), Expect = 2.530e-126
Identity = 262/648 (40.43%), Postives = 363/648 (56.02%), Query Frame = 0

Query:  224 CDFLSVAVDASPRGHKFRLDL---MELAKRDA----DGTCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQ----IDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFI 860
            C F       SP G K  L     M  A  D     D  C +AL+A+ SLQP V  + A+ R     N DA W               V QS      P+W   + G  E+IG+AD+G+D         HC F + DG  ++AS W+ PVTD  +RK++QYI +VD  DE +GHGTHVAG++ GA+ G  AG    DG AF+GK+ VFDFG SD  ++L+ P ++ +++L P+Y+ GARVHSNSW T S     +  E+D +V  HQ ML+V AAGNCGD S   L         +  +V SPAQ KNVVAVG+S +    V G D++T+++FS++GPT+DGRIKPDVV PG    SA+A+T G   +C   S+ GTSM+TP+VA + A+ RQYFREGWYN+G +    E+ GF PS ALLKAVL+NSA  ++     P +                                                     +L +T L  PPD  QG GR+ +   +N+++TVG+FF+DW +I EG+ ++Y+V+    A T + L +TLVWTDPP++ G+ + ++H L + V  + +    YPNGL +AD VNNV+K+ +  T QG  YII VS   ++E  TQ +A+ A GEFI
Sbjct:  345 CGFDLARFTVSPSGKKVLLHRPHEMVAAANDXXXXXDEGCLSALLAFFSLQPEVRYVTAR-RKTATQNLDAAW---------------VTQSAVDGYTPLWDENLQGQDEIIGVADTGLD-------LNHCQFREDDGDNIEASDWDDPVTDLDKRKVVQYIDFVDDFDEADGHGTHVAGSICGAQSGEDAGDDDADGMAFQGKLAVFDFGDSDNFNALTTPDEIDSVILEPAYDGGARVHSNSWDTVSTEYTALSNEVDEFVYTHQNMLVVFAAGNCGDTSTDDLIDNCSVLDDDEGTVLSPAQGKNVVAVGSSES--GGVTGKDMDTVSYFSSKGPTIDGRIKPDVVAPGDPTWSAAADTTG--ETCEFGSQTGTSMSTPIVAGNGAMARQYFREGWYNTGSK---NESLGFDPSGALLKAVLINSATGMSFAGVNPDSG----------------------------------------------------TLDVT-LTTPPDTHQGFGRVTMINALNINSTVGIFFEDWVEIREGETIDYDVSLYEDADTDKDLRITLVWTDPPASVGSTEALIHDLDLVVIAESTGTEYYPNGLEEADTVNNVEKITITDTTQGENYIIRVSCNELSEYDTQNFAVIANGEFI 909          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: D7FS58_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS58_ECTSI)

HSP 1 Score: 296 bits (759), Expect = 3.860e-82
Identity = 217/613 (35.40%), Postives = 305/613 (49.76%), Query Frame = 0

Query:  256 CTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFY-DSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWG----TQSQIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGD-INTIAFFSARGPTVD-GRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTT--VPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWT--YPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAG 857
            C   ++AY+  +P V  ++   +   LN  +A W               + QSG    + MW+ GI G+ E+I MADSG+D          C F  D     +  S+W+ PV D T+RKI+QY+ +VD  D +NGHGTHVAG+  G    ++ G    DG  F  K+ VFDFG S+G   L  P  +++ +L P Y+ GAR+ +NSWG    T + +D ++D +   +Q M+IVIAAGNCGD  +       +  G  S S+ SPA  KN+++VGAS   P+     D ++ +A+FS++GPTVD  RIKPDVV PGY L SASA  P    SC      GTSMATPVVA +AA++RQYF +GWY SG   A   N  F PS AL+KA++V S  A+ + +                                                          S G T L  PPD  QG GR+LL+  +NV  +V LF +D   +  G V  + +    V ++     + VT+VW +P    G+  PV + L ++V     T  +  YPN LA  D  N V+ VV+P       + + V+A  VT +   YYAL   G
Sbjct:  197 CLLTVLAYLVTRPEVSYVDDLPQVFELNV-EAAW---------------ITQSGEETAYSMWNEGIDGSTEIIAMADSGLDV-------NSCFFTEDLTDDNIDCSSWSNPVFDLTKRKIVQYVGFVDCVDTENGHGTHVAGSAAGGLSNSSPGEHFGDGAGFGAKLAVFDFGNSEG--GLVTPSSLKSQMLQPPYDIGARISTNSWGAVRTTYTSLDRQVDDFAYLNQDMVIVIAAGNCGDEISGCQYQNADIYG--SGSILSPALGKNIISVGASEASPSTAGLADNVDEVAYFSSQGPTVDDNRIKPDVVAPGYHLFSASAK-PDQESSCEIGPMAGTSMATPVVAGAAAMIRQYFVQGWYPSG--IATPSNI-FNPSNALVKAMIVTSGTAMASYDS---------------------------------------------------------SAGSTTLGSPPDAIQGFGRVLLDSVLNVRGSVDLFVEDRVDMTHGDVNSHFIEMDGVSSSAFDGDMKVTMVWMEPAGFSGSVAPVFNDLDLKVVAYDGTSESTFYPNNLAAKDPDNTVEMVVIPDVDSYEWFNVTVTAASVTTTSLPYYALVVTG 721          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A835YK17_9STRA (Subtilisin (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YK17_9STRA)

HSP 1 Score: 281 bits (718), Expect = 2.790e-78
Identity = 218/569 (38.31%), Postives = 291/569 (51.14%), Query Frame = 0

Query:  305 VAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGII-VKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTA--GSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWG----TQSQIDVEMDTYVNDHQQMLIVIAAGNCGDV-SNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVD-GGDINTIAFFSARGPTV-DGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGG-QAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQI-AEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVRE-QGSTVWTY-PNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQ--YYALAAAG 857
            + QSG    +PMW+AG+    E+I +ADSGVD          C F + D    +  S +  PVTD  +RK++QY+ YVD  DE+ GHGTHVAGTL GA+       G  + DG AF  K+ VFDFG  D   +L  P D+   +LA  Y AGARV SNSWG    + + +D ++D +       ++V+AAGNCGD  S  + D G    G  S  V SPA AKNV+AVGAS +   +   GG +  +A+FS++GP   DGRIKPDVV PGY   SA AN    G SC  A++ GTSMATPVVAA+AA++RQYF +GWY SG    +D  N    PS AL+KAVLVN+A         P  A                                        G+ +  P G+            PD  QG GR+LL+  +NV  +V +  +D   +   G V  Y  T    A        T+V+ +P  A GAA PVL+ L + V    G T   + PNG   AD VN V++V +P  +    + + V+A  V  +PTQ  ++AL   G
Sbjct:   57 IVQSGVEGAYPMWTAGLQADTEIIAVADSGVD-------LASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFG--DAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGS--VLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPG-SCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHN----PSGALVKAVLVNAA--------VPMTAYR--------------------------------------GLAATVPLGTG-----------PDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTY--TFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVA-TPTQPPFFALVVTG 549          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: K8ZC45_NANGC (Subtilisin n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=K8ZC45_NANGC)

HSP 1 Score: 268 bits (684), Expect = 5.170e-72
Identity = 214/622 (34.41%), Postives = 296/622 (47.59%), Query Frame = 0

Query:  256 CTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSG-------SLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQS-----QIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLN-----MGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFI 860
            C  AL+A+VS  P V  +E     +L N   A                 + QSG       + + +P++  G+ G  +V+ + D+GVD       +  C F D+ G +   +   A   D++RRKI+QY+ + DS D +NGHGTHVAGT+ G    T+   +  +G A   K+  FD G S G   L++P +  T L  P Y AGAR+ SNSWGT S       D E+D ++ ++   L++++ GN G           E  G     V SPA +KNV++VGAS +  N     D   +A+FS+ GPT DGRIKPD+V PG  L SA++     G +C    +QGTSMA PV A SAA+VRQYF EGWY  G + A +   GF+P+ ALLKA+L+NSA  +                                              A  F  G+  P      LGL     PPD  QGHGRI L        +    T  L+  +   I E +   Y  +   T  +   + +TLVW DP +   +A  VLH L + V  Q      +PNG   AD+VN V+KV L S   G    + V+A  V   P Q YALAA+G F+
Sbjct:  371 CLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAH----------------LTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVD-------QNSCFFSDAKGPLPTTTISEAAF-DSSRRKIVQYVQWADSTDVENGHGTHVAGTMAGVSLDTSMDMSSYEGVARGAKLAFFDMGFSSG--GLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKGR----------EGIG----GVASPALSKNVLSVGASLS--NRYADLDPTHVAYFSSWGPTKDGRIKPDIVTPGRRLVSAAS-----GATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAAD---GFVPTGALLKAMLINSAVPM---------------------------------------------AAFDFNNGTLLP------LGL-----PPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKFSIPATGKSP--VKITLVWIDPDTTASSATTVLHDLDLMVYSQKEGSSYFPNGRTNADNVNTVEKVALYSPTPGDTLTVTVTATSVATRPEQRYALAASGNFV 884          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A4D9CUI1_9STRA (Subtilisin n=2 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CUI1_9STRA)

HSP 1 Score: 268 bits (686), Expect = 2.310e-71
Identity = 224/673 (33.28%), Postives = 312/673 (46.36%), Query Frame = 0

Query:  223 ACDFLSVAVDASPRGHKFRLDLMELAKRDADG------------------TCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSG-------SLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQS-----QIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLN-----MGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGEFI 860
            +CDFL+V   AS +    RL   +     ADG                   C  AL+A+VS  P V  +E     +L N   A                 + QSG       + + +P++  G+ G  +V+ + D+GVD       +  C F D+ G +   +   A   D++RRKI+QY+ + DS D +NGHGTHVAGT+ G    T+   +  +G A   K+  FD G S G   L++P +  T L  P Y AGAR+ SNSWGT S       D E+D ++ ++   L++++ GN G              G     V SPA +KNV++VGAS +  +     D   +A+FS+ GPT DGRIKPD+V PG  L SA++     G +C    +QGTSMA PV A SAA+VRQYF EGWY  G + A +   GF+P+ ALLKA+L+NSA  +                                              A  F  G+  P      LGL     PPD  QGHGRI L        +    T  L+  +   I E +   Y ++   T  +   + +TLVW DP +   +A  VLH L + V  Q      +PNG   AD+VN V+KV L S   G    + V+A  V   P Q YALAA+G F+
Sbjct:  323 SCDFLNVG--ASIQYPYLRLSGFDNLALKADGKRRLEQAYDSNSTISDQSACLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAH----------------LTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVD-------QNSCFFSDAKGPLPTTTISEAAF-DSSRRKIVQYVQWADSTDVENGHGTHVAGTVAGVSLDTSMDMSSYEGVARGAKLAFFDMGFSSG--GLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKG--------------GEGIGGVASPALSKNVLSVGASLS--DRYADLDPTHVAYFSSWGPTKDGRIKPDIVTPGRRLVSAAS-----GATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAAD---GFVPTGALLKAMLINSAVPM---------------------------------------------AAFDFNNGTLLP------LGL-----PPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKLSIPATGKSP--VKITLVWIDPDTRASSATTVLHDLDLMVYSQKEGSSYFPNGRTNADNVNTVEKVALYSPMPGDTLTVTVTATSVATRPEQRYALAASGNFV 885          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: W7TM23_9STRA (Subtilisin n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TM23_9STRA)

HSP 1 Score: 267 bits (683), Expect = 9.650e-71
Identity = 223/705 (31.63%), Postives = 310/705 (43.97%), Query Frame = 0

Query:  246 ELAKRDADG-TCTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSG--SLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGA-------------RDGTTAGST----------------------------------------------------------ITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQSQI----DVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFL------PSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEY--------NVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGE 858
            E A+ +AD   C   LVA+++  P +  ++ + R  L N++ A W               + QSG  + +  P+W  G+ G GE+IG+AD+G+D          C FYD+    V  S+W AP TD T+RK++QY+++VD  D   GHGTHV+G+L G               +G T   T                                                             G A E KI  FD     G  +LS P D   ML  P+Y AGAR+HSNSWG         D+++D Y  +HQ  LI+ AAGN G+    S+               SP   KNV+ VGA+ + P      D + IA FS+RGPT DGR+KPD+V PG  + SA +     G +C+  +  GTSMA PVVA +AAL+RQYF+EG Y +  +A  E    +       PSA LLKA+ VNSA  V  +  +                                                           L AL  PPD AQGHGRI ++  ++      LFF D Q +A G+   Y        N ++VP       L VTL W DPP++  A K +LH L + V +    ++ YPNGL   D  NNV+K+V+     G +Y + V+A  ++E+  Q YAL A G+
Sbjct:  412 EGAEDEADRQACLEGLVAFIASHPDILSIQTRARYALQNDQ-ATW---------------IIQSGDEATHARPLWDLGLTGKGELIGVADTGLDD-------ESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVEPRNEGRRGHFCAEGETLACTGMCLSAPTCQLKDYGYVSCQDWLQDGHCDAWFACPLYGCDGGDCVGLACNPNPEEALMANQGMAREAKIAFFDIADEHG--NLSTPSDFHRMLFPPAYEAGARIHSNSWGINVGEYLFDDMKIDQYAYEHQDFLIIYAAGNYGEDGFHSIG--------------SPGNTKNVLTVGATESGPER-QSQDRDYIAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGGTCSYVAMAGTSMAAPVVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDVIQPSAPLLKALFVNSAVPVKALRLEDE---------------------------------------------------------LVALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGESRTYYFQIAAASNASSVP-------LQVTLCWIDPPNSFTAQKQLLHDLDLHVLDPSGVLF-YPNGLPGPDRKNNVEKIVVVEPLPGGVYTLVVTAGELSEAEEQLYALVATGD 1011          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A4D9D243_9STRA (Subtilisin n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D243_9STRA)

HSP 1 Score: 265 bits (678), Expect = 1.740e-70
Identity = 229/706 (32.44%), Postives = 309/706 (43.77%), Query Frame = 0

Query:  246 ELAKRDADG-TCTAALVAYVSLQPHVYRLEAQHRAIL-LNNRDADWAXXXXXXXXXXXXXXVAQSG--SLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGA-------------RDGTTAGST----------------------------------------------------------ITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGT---QSQID-VEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFL------PSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEY--------NVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGSTVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAGE 858
            E A+ +AD   C   LVA+++  P +  L  Q RA   L N  A W               + QSG  + +  P+W  G+ G GE+IG+AD+G+D          C FYD+    V  S+W AP TD T+RK++QY+++VD  D   GHGTHV+G+L G               +G T   T                                                             G A E KI  FD     G  +LS P D   ML  P+Y AGAR+HSNSWG    +   D +++D Y  +HQ  LI+ AAGN G+    S+               SP   KNV+ VGA+ + P      D N IA FS+RGPT DGR+KPD+V PG  + SA +     G +C+     GTSMA P+VA +AAL+RQYF+EG Y +  +A  E    +       PSA LLKA+ VNSA AV  +  +                                                           L AL  PPD AQGHGRI ++  ++      LFF D Q +A G+   Y        N ++VP       L VTL W DPP++  A K +LH L + V +  S V  YPNGL   D  NNV+K+V+     G +Y + V+A  ++E+  Q YAL A G+
Sbjct:   85 EGAEDEADRQACLEGLVAFIASHPDI--LSIQTRATYALQNDQATW---------------IIQSGDEATHARPLWDLGLTGKGELIGVADTGLDD-------ESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVEPRNEGRRGHVCAEGETLACTGMCLSAPTCQLKEYGYVSCQDWLQDGHCDAWFACPLYGCDGGDCVGLACNPNPEEALMANQGMAREAKIAFFDIADEHG--NLSTPSDFHRMLFPPAYEAGARIHSNSWGINVGEYLFDAMKIDQYAYEHQDFLIIYAAGNYGEDGFHSIG--------------SPGNTKNVLTVGATESGPER-QSQDRNYIAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGGTCSYVGMAGTSMAAPLVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDVIQPSAPLLKALFVNSAVAVKALRLEDE---------------------------------------------------------LVALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGKSRTYYFQIAAASNASSVP-------LQVTLCWIDPPNSFTAQKQLLHDLDLHVLDP-SGVRFYPNGLPGPDSKNNVEKIVVVEPLPGGVYTLVVTAGELSEAEEQLYALVATGD 684          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A4D9D260_9STRA (Subtilisin n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D260_9STRA)

HSP 1 Score: 257 bits (656), Expect = 1.800e-67
Identity = 222/651 (34.10%), Postives = 313/651 (48.08%), Query Frame = 0

Query:  223 ACDF--LSVAVDASPRG-HKFRLDLMELAKRDADGT-CTAALVAYVSLQPHVYRLEAQHRAILLNNRDADWAXXXXXXXXXXXXXXVAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWGTQ----SQIDVEMDTYVNDHQQMLIVIAAGNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVDGGDINTIAFFSARGPTVDGRIKPDVVGPGYSLTSASANTPGGGP--SCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLN--MGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTT-RLLSVTLVWTDPPSAPGAAKPVLHTLGVRV-REQGSTVWTY-PNGLAQADD-VNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQYYALAAAG 857
            ACD+  L+++V+      H +   L +  +R  D T C  +LVAY +  P V  LE      ++   DA  A                QSG     P+W AG+ G+G++I +AD+GVD          C F D  G +   + W+A + D +RRK++Q +   D+ D   GHGTH AGT +G     +  +    G A+  K+  +D G ++G   L  P  +Q  + AP Y+AGARV SNSWG+     + +D E D ++      LIV++AGN G                 S +VFSP+ +KN + VGAS +   +     +  I  FS +GPT DGRIKPD+V PG  LTSA  N+  G P  +C+  S+ GTSMAT VVA S AL+RQ+F EG Y SG     +    F+P+ ALLKA+L+NSA  +    Q                                                   P G + +LGL     PPD  QG GR+ L+  + +     V LF +D   +AE +   Y  T   T  +T +   VTL WTDP     A   VLH L +R+ R  GSTV TY PNGL    D +N+V+KVVL +   G +Y + V+   ++ +  Q Y+LA AG
Sbjct:  293 ACDWSSLNISVELPYLSVHGYCSVLPDPVERLVDATACLTSLVAYFASHPEVIFLETFP---IVKPSDAIAAA-------------TVQSGEQTSTPLWDAGLDGSGQIIQVADTGVD-------MTSCFFSDPTGNVAP-TRWDAGMYDLSRRKVVQLVVGEDAFDTPAGHGTHCAGTALGNSATPSRFNLNFRGVAYMAKLAFYDGGTANG--GLKFPASMQMKMFAPGYHAGARVFSNSWGSNQPKYTYMDTEFDGFMYSQPDALIVVSAGNLG--------------ARGSGTVFSPSLSKNALCVGASQSLRRSSY--RVENIPHFSGQGPTFDGRIKPDLVAPGAFLTSA--NSSSGDPRYACSVTSKSGTSMATAVVAGSVALLRQFFLEGRYPSGAPNPPD---AFVPTGALLKALLINSAVPMERYIQ---------------------------------------------------PNGQSIALGL-----PPDNYQGFGRLQLDIFLPLKPQNPVILFVRDAVPLAEEETHTYKFTVPDTRTSTLKPFHVTLTWTDPTVPASAGAIVLHDLDLRLSRWLGSTVETYYPNGLTNTPDRINSVEKVVLYAPIPGDVYTVTVTGTSISTTQYQNYSLAVAG 840          

BLAST of jgi.p|Trimin1|349462 vs. uniprot
Match: A0A835Z5P2_9STRA (Subtilisin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z5P2_9STRA)

HSP 1 Score: 249 bits (636), Expect = 7.480e-65
Identity = 216/640 (33.75%), Postives = 291/640 (45.47%), Query Frame = 0

Query:  305 VAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSD-----------GIIVKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTAGSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYN-AGARVHSNSWGT--------------QSQIDVEMDTYVNDHQQMLIVIAA------------------------------------GNCGDVSNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVD-GGDINTIAFFSARGPTV-DGRIKPDVVGPGYSLTSASA----------NTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGGQAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGPPDKAQGHGRILLNMGVNVDATVGLFFKDWQQIAEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVREQGS----TVWTYPNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPV-------VTESPTQ--YYALAAAG 857
            +AQS      P+WSAG+ G GEVI ++DSGVD+         C F +                V+ S + +PVTD  RRKI+QY+ + D  D   GHGTHVAGTL GA+ G TAG  + DG AF+ K+ VFD GASDGT  L  P D++  +L PSY  AGAR+ +NSWG                + +D +MD +  ++   ++V+AA                                    GNCGD  +       +  G  S  V SPA AKNV+AVGAS +   +   GGD++ +A+FS++GPT  DGRIKPD++ PGY   SA A                  C  A+  GTSMA PVV+A+AAL+RQYF +GWY SG     +    + PS AL+KA+LVN A  +         AR                                              RG A S+ L A    PD  QG GRI L+  +++  +V L   D   +     V     TV         + T+V+ +PP A GA  PVL+ L + V    S    TV+ YPNG A  D VN V++VV+          +     V        + SP Q  ++ALA  G
Sbjct:  312 IAQSARRGATPLWSAGLRGGGEVIAISDSGVDA-------ASCFFTEEPRXXXXXXXXXXXXAVQCSNFTSPVTDLRRRKIVQYVGFADCADAPGGHGTHVAGTLAGAQAGATAGRHLGDGIAFDAKLAVFDLGASDGT--LLTPADLKAQMLTPSYKGAGARISNNSWGAIXXXXXXXXXXXXMYTTLDRQMDMWAYNNPDAVLVVAATFPTPSQYPHPRXXXXXXXXXXXXXXXXXXXXXXXAGNCGDKGSGCAGGAGQLAGAGS--VMSPALAKNVIAVGASESALGSAALGGDVDNVAYFSSQGPTPGDGRIKPDILAPGYHTVSAEAAXXXXXXXXXXXXXXXXXCRVAAMAGTSMAAPVVSAAAALMRQYFVQGWYPSGRPNPSDV---YNPSGALVKALLVNGAAPM---------ARY---------------------------------------------RGRAESVTLGA---GPDGYQGWGRIQLDAALDIGGSVDLHVVDRVYVRAANFVATYSYTVRDGYDGDFRA-TMVFVEPPGAVGAVSPVLNDLDLIVTAYDSAARPTVF-YPNGGAAPDAVNTVERVVVKRDTAAARAFVAFDVTVRAAALVTASASPPQPPFFALAVTG 878