Trimin1|346771|estExt_Genemark1.C_Ctg_50037 (polypeptide) Tribonema minus UTEX_B_3156

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A835YUB7_9STRA (Isoleucyl-tRNA synthetase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YUB7_9STRA)

HSP 1 Score: 2565 bits (6647), Expect = 0.000e+0
Identity = 1582/1582 (100.00%), Postives = 1582/1582 (100.00%), Query Frame = 0

Query:    1 MADGEDGAPSGQLREPQVEDLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPALSVARAFAARAAAAGLDGSVAAALLASVDVHGXXXXXXXSAELPPQLELTLDGKTVVARCSEDYFGSALALLRGGGGDGAGLEWAL 1582
            MADGEDGAPSGQLREPQVEDLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPALSVARAFAARAAAAGLDGSVAAALLASVDVHGXXXXXXXSAELPPQLELTLDGKTVVARCSEDYFGSALALLRGGGGDGAGLEWAL
Sbjct:    1 MADGEDGAPSGQLREPQVEDLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPALSVARAFAARAAAAGLDGSVAAALLASVDVHGXXXXXXXSAELPPQLELTLDGKTVVARCSEDYFGSALALLRGGGGDGAGLEWAL 1582          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: D7FTL9_ECTSI (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTL9_ECTSI)

HSP 1 Score: 1285 bits (3324), Expect = 0.000e+0
Identity = 746/1560 (47.82%), Postives = 898/1560 (57.56%), Query Frame = 0

Query:   18 VEDLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSE--VGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTA-----TITLTRPALSVAR-AFAARAAAAGLDGSVAAALLASVDVHGXXXXXXXSAELPPQLELTLDGKTVVARCSEDYFGSALALLR 1569
            ++D+   ++F AEE+ V A W+E D F+ + +         LSEGRP+FTFYDGPPFATGLPHYGH+LAGTIKD VTRYAHQ+G+ V RR GW CH              GLPVEYEIDQ+LGIK RD VL MGI TYN HCR          IV+RY+KEWE T+KRLGRWIDF NDYKTMDP FMESVWWVF++L EK LVYRGYK                    VMPFSTACATPLSNFEAGLNYKDV+DP++VVAF ++DDP+ SLLAWT+TPWTLPSNLALCVN  F+Y+K+                                                       RD KSGK +              DRLVQ+YPIM TKK+KP M AE  E L T+KGS LVG+ YEP+F +F DR  SF V  D+YVT + GTGIVHQ+PAFGEDDYRVCLAH V+AKGE               +PCPVD+NG FT                  ++ G++VK AD  +C  +KA GRL+ KD  THSYP+CWRSDTPLIYKAVPSWFV VESI+D+LL  N  TYWVPA V EKRF NWL  A                           KDWA+SRNRFWGTP+P+W SEDGEE +A+GS+ +L ELSGV+                                                                    +TDLHRES+DHITIPSKQGKG L+RV+EVFDCWFESGSMPYAQLHYPFENK++FE  FPADFIAEGLDQTRGWFYTL+VL  ALFDKP FKNL+ NGLVLA DGKKMSKRLKNYPDP                                      VI ++GADALRLYLI+SPVVRAE L+F+E GVQA V++VLLPWFN  RFFVQQ                                   ARR E     G  F P+PEAAA STNVMD W+ A+LQGLVQFVH EM AYRLYTVVPRLV FI+ LTNWYVRLNR+RLKG++G E A  GL C+YEVL  MS +MAP  PFFSEY Y+ LR+ H +    DG    +E   G A SVH LM+P+ +   L+ QAE+DM AMQ                   VI+L R+AR+KR ISLK PVK + VV KD+  L A+EKL GYV+GE+N W+V   +DE +WCTL+A+ NNK+LG+R GK    +K+ + +   A L   +                               GT++VAG TLSA++L+++R F+GD +V++A VS+DG V+VVLDTR+DE+VL Q+ ARE ++R+QKLRK                      +GL +G+ VEVFF +        AA+AA+ A++ DA   +PLPA+   P HA  +  + F     D   G  A        T+ LTRP LSV   A  A+  AA +D +VA  LLAS+D          +  L      T+    V      DYF  A   LR
Sbjct:   14 MKDIVGAMDFPAEEELVAASWEENDTFKETLR---------LSEGRPDFTFYDGPPFATGLPHYGHILAGTIKDAVTRYAHQSGHHVSRRAGWDCH--------------GLPVEYEIDQSLGIKHRDQVLEMGIETYNKHCRS---------IVTRYSKEWERTIKRLGRWIDFENDYKTMDPWFMESVWWVFKSLVEKNLVYRGYK--------------------VMPFSTACATPLSNFEAGLNYKDVQDPAVVVAFPLKDDPEVSLLAWTTTPWTLPSNLALCVNKAFDYVKL-------------------------------------------------------RDVKSGKVYYC----------GADRLVQLYPIMATKKYKPAMKAELMEELGTVKGSELVGLKYEPMFKFFADREQSFVVCEDNYVTNESGTGIVHQSPAFGEDDYRVCLAHGVIAKGEE--------------IPCPVDSNGLFTSEVG--------------EYAGQYVKTADKALCDEIKANGRLVSKDSCTHSYPFCWRSDTPLIYKAVPSWFVGVESIRDKLLKANAETYWVPAFVNEKRFQNWLEGA---------------------------KDWAVSRNRFWGTPMPMWVSEDGEEMVAVGSIAELKELSGVE--------------------------------------------------------------------VTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQLHYPFENKDKFEKNFPADFIAEGLDQTRGWFYTLTVLGAALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYPDP------------------------------------MKVIDSHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQ-----------------------------------ARRLEMTC--GDRFVPNPEAAAKSTNVMDNWIQATLQGLVQFVHTEMKAYRLYTVVPRLVEFIEQLTNWYVRLNRNRLKGAEGAESATAGLFCMYEVLSTMSVLMAPITPFFSEYTYRHLRECHPD---KDGGKDVAEDAPGRASSVHMLMMPEVDESRLDPQAESDMRAMQT------------------VIDLGRSAREKRGISLKTPVKGITVVCKDEGTLKALEKLQGYVKGELNAWEVVLEADEKSWCTLTAEANNKVLGKRLGKALDGVKKALVKLDTAALWPLLEG-----------------------------GTVEVAGHTLSADDLMLKRGFKGDTSVFQAAVSEDGKVMVVLDTRKDEKVLSQKLAREIVSRIQKLRKK---------------------SGLQVGETVEVFFQDDTKDGVAGAAVAANAALVADAVRCMPLPASRM-PEHAVPLGHERFCACGGDDGKGEEAAADQEPDFTVFLTRPCLSVDHEAMDAKCVAAKVDPAVARQLLASLDYDRVRREGNATTVL------TVGEACVTVDLGADYFLDAREALR 1182          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: W7U130_9STRA (Isoleucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U130_9STRA)

HSP 1 Score: 1218 bits (3151), Expect = 0.000e+0
Identity = 710/1507 (47.11%), Postives = 851/1507 (56.47%), Query Frame = 0

Query:   20 DLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYE-ILETIKGSALVGIAYEPLFPYF---------------KDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGV--LRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSS-------EVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAV-EKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFF-SESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPALSV 1499
            ++ A+ NFA  E+++L  W ++DAF+ S K         LSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTR+AHQ G+ V RRFGW CH              GLPVE+EIDQ LGIK RD V AMGIA YN HCR          IV+RYTKEWE TV R+GRWIDF NDYKT+DP FMESVWWVF  LH+KGLVY+GYK                    VMPFSTAC TPLSNFEAGLNYKDV+DP+++V+F +  DP  SLLAWT+TPWTLPSNLALCVNPE  YIK+                                                       RD KSGK +++          ++ RLVQ+YP + + K K E   E YE I E   G+ + G  Y PLF YF               ++  G+F VL D+YVT+D GTGIVHQAP FGEDDYRVCLAH V+ KG                VPCPVD+NGRFT                  DF G+HVK AD DIC  LKAKGRL+ K  Y+HSYP+CWRSDTPLIYKAVPSWFVAV ++K++LL NN+ TYWVPA VKE RFHNWL+                           DAKDWAISRNRFWGTPIPLW SED EE++AI S+EQL  LSGVK                                                                    ITDLHRES+D ITIPS +G     LRRVDEVFDCWFESGSMPYAQLHYPF+NKERFEA FPADFIAEGLDQTRGWFYTL V+ST LFDKPPF+NL+ NGLVLA DGKKMSKRLKNYP P+++                                    +  YGADALRLYLI SPVVRAEPLRF+E  V  ++K V LPW N  RFF Q                      GV R     GS+               F PSPE A ASTNVMD+W+ ASLQG+++FVH EMAAYRLYTV PRL+ FI++LTNWYVRLNR+RLKG +G EE+L GL+ LYEVLL M+ +MAPF PF +E++YQ LRK+H N+T    A  SS        +G A SVHY+MLP ++   LN   E  MA +Q                   VIEL R  R++ NI+LK PVKE+IVV+KDQ  L+ + E + GY   E+NVW + TS + G W  +  +PNNKLLG+R GK  A +   IA  T   + AF                               TG++ +AG  LS+EELL+++ F+GD ++++A  S DG ++VVLDTR+DERVL Q TARE +NRVQKLRK                       GL + D VE+++ +E  G   +  AIA++ AM+R      PLPA+  +P HA  +  +          C    V  T+ +TRP LS+
Sbjct:    2 EVEASHNFAQAEEEILKLWTKLDAFEQSLK---------LSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRFAHQKGHHVVRRFGWDCH--------------GLPVEFEIDQELGIKDRDQVFAMGIAKYNQHCRS---------IVTRYTKEWERTVTRIGRWIDFKNDYKTLDPWFMESVWWVFSALHKKGLVYQGYK--------------------VMPFSTACGTPLSNFEAGLNYKDVQDPAVIVSFPLLSDPSTSLLAWTTTPWTLPSNLALCVNPEIRYIKV-------------------------------------------------------RDKKSGKQYVV----------AEARLVQLYPKLASGKVKEEQKQEWYEQIGEAFLGAEMAGWGYTPLFDYFVEWNEGERAGKEGDGRENLGAFRVLVDAYVTSDSGTGIVHQAPGFGEDDYRVCLAHGVIRKGTN--------------VPCPVDSNGRFTPDVR--------------DFVGQHVKAADGDICQLLKAKGRLVDKATYSHSYPFCWRSDTPLIYKAVPSWFVAVGTVKEKLLANNQKTYWVPAFVKEHRFHNWLA---------------------------DAKDWAISRNRFWGTPIPLWCSEDMEEQVAIESIEQLERLSGVK-------------------------------------------------------------------GITDLHRESVDQITIPSSRGPSYPPLRRVDEVFDCWFESGSMPYAQLHYPFDNKERFEANFPADFIAEGLDQTRGWFYTLMVISTCLFDKPPFQNLIVNGLVLASDGKKMSKRLKNYPPPDDI------------------------------------LKKYGADALRLYLISSPVVRAEPLRFKEEDVLGMIKRVFLPWINAFRFFQQ----------------------GVARLEMEKGSV---------------FTPSPERARASTNVMDVWIQASLQGMIKFVHDEMAAYRLYTVTPRLLRFIEELTNWYVRLNRTRLKGQEGDEESLTGLSTLYEVLLSMTILMAPFTPFLTEFLYQHLRKIHPNFTSCSAAASSSVSALPLDAIGKADSVHYIMLPMYDEARLNPLCEERMATLQT------------------VIELGRMVRERSNINLKTPVKEVIVVSKDQGKLAHLDESVRGYALQELNVWSLKTSDEVGKWSNVHVEPNNKLLGKRLGKDRAKVAAAIAALTPEDIEAF-----------------------------QNTGSLTLAGYELSSEELLLKKEFKGDTSIFQAEASQDGTLLVVLDTRQDERVLAQATARELVNRVQKLRKR---------------------VGLKLSDVVEIYYETEDKG---MIEAIASNAAMVRSTLRVFPLPASTLKPTHAVELGKE---------ECLINDVAVTVYVTRPTLSL 1116          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A7S2WSX5_9STRA (Isoleucine--tRNA ligase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WSX5_9STRA)

HSP 1 Score: 1157 bits (2994), Expect = 0.000e+0
Identity = 664/1442 (46.05%), Postives = 818/1442 (56.73%), Query Frame = 0

Query:   20 DLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPG--SFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNA--KLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIA--AHVAMIRDATGSVPLP 1455
            +LPAN++F  EE++VLAFW EIDAF+T  +         L+ G+PE+TFYDGPPFATGLPHYGH+LAGTIKDTVTRYA QTG+ V RRFGW CH              GLPVE+EID+TLGI +RD V  MGI  YN+ CR          IVSRYTKEWE TVKRLGRWIDF NDYKTM+P FMESVWWVF+ L  KGLVY+GYK                    VMP+STAC TPLSNFEAGLNYK+V+DP++VV+F + +DP  SLLAWT+TPWTLPSNLALCVN EF+Y+KI                                                       +D K+ + +IL           + RLVQ+YP+MG KKWKPE  +  + +LE IKG  LVG  Y PLF YF D  G  +F V+ D YVTAD GTGIVHQAPAFGEDDYRVCL H V+AKG                +PCPVD NG +T                  DF+G+HVK+AD  IC  LKA+GRL+   +Y HSYP+CWRSDTPLIYKAVPSWFVAVE  KDRLL NNE TYWVP  VKE RFHNWL                            +A+DWAISRNRFWGTPIP+W SED +E IAIGSV +L EL+G+                                                 +G                  +TDLHRESID I IPSKQGKG LRRVDEVFDCWFESGSMPYAQLHYPFENKERFE GFPA FIAEGLDQTRGWFYTL VLSTALFD+P F NL+ NG+VLA DGKKMSKRLKNYPDPN                                     VI  YGADALR+YLI+SPVVRAEPL+F EPGV+ V +DV+LPW+NT RFF+Q                                    A RW+   A G  F+P    A++S N +++W+ A LQ L+ FVH+EM+ YRLYTV+PRLV+F++ L+ WY+RLNR RL+G+DG EEALVGL+ LY V++ ++ +M+PF PFFSE++YQ+LR+LH ++   D       +G+AQSVH+L LP+  A    LN +   +MAA+Q                   VIEL R A++ +NIS K PVKE+I+VT +    + + KL  Y+  E++  ++T +  E  WC+  A PN K LG+R GK    +K+ I + +++ ++ F                              ATG + V    L   +L V R F GD A +    S+DG +V+ +DT  D  +++   +RE ++ VQKLRK                      A L +GDK+EVFF E  G T ++AA+   A    +    G  PLP
Sbjct:   14 ELPANLSFPEEEERVLAFWAEIDAFKTQLE---------LTRGKPEYTFYDGPPFATGLPHYGHILAGTIKDTVTRYASQTGHFVERRFGWDCH--------------GLPVEFEIDKTLGITNRDQVFEMGIDKYNAECRS---------IVSRYTKEWESTVKRLGRWIDFENDYKTMNPWFMESVWWVFKQLFAKGLVYQGYK--------------------VMPYSTACGTPLSNFEAGLNYKEVEDPAVVVSFPLEEDPSVSLLAWTTTPWTLPSNLALCVNVEFDYVKI-------------------------------------------------------QDVKTERLYILL----------ETRLVQLYPVMGGKKWKPEKNSTLFTVLEKIKGKDLVGKRYTPLFRYFVDSFGESAFRVIEDGYVTADGGTGIVHQAPAFGEDDYRVCLKHGVIAKG--------------VQLPCPVDFNGCYT--------------AEVSDFEGQHVKQADAAICDMLKAQGRLVASAKYLHSYPFCWRSDTPLIYKAVPSWFVAVEQFKDRLLKNNEQTYWVPNFVKEGRFHNWLK---------------------------EARDWAISRNRFWGTPIPIWASEDMQELIAIGSVAELEELAGLP------------------------------------------------KGS-----------------VTDLHRESIDDIVIPSKQGKGNLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEKGFPAHFIAEGLDQTRGWFYTLMVLSTALFDRPAFSNLIVNGMVLAADGKKMSKRLKNYPDPN------------------------------------AVIATYGADALRMYLINSPVVRAEPLKFTEPGVKGVARDVILPWYNTFRFFLQN-----------------------------------AHRWQL--ATGKTFRPDAALASSSANPINVWIRAELQSLIAFVHEEMSKYRLYTVMPRLVSFLEQLSKWYLRLNRDRLRGADGEEEALVGLSVLYFVVISVASIMSPFTPFFSEFLYQKLRQLHPDFAKPD----KEAMGSAQSVHFLTLPRPEAVDPGLNARVAEEMAALQT------------------VIELGRKAKEAQNISHKKPVKEVIIVTCNPVHAAGLHKLQNYLESELSALKITVTDKEDQWCSYKAIPNFKELGKRCGKQMQQVKKAIVELSNSDMATF-----------------------------RATGELKVLDFDLGRTDLAVTRHFAGDKAKFAHMESEDGSMVIAIDTEEDAGIIRSWVSRELVSTVQKLRKV---------------------ALLEVGDKIEVFFREI-GDTQIAAALEDPAIATAVEQKLGCRPLP 1072          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A7S3XRA7_HETAK (Isoleucine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XRA7_HETAK)

HSP 1 Score: 1105 bits (2859), Expect = 0.000e+0
Identity = 647/1369 (47.26%), Postives = 763/1369 (55.73%), Query Frame = 0

Query:  101 GYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLA---MGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRP---GSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEAL--DAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQP 1461
            G+ V RRFGW CH              GLPVEYEIDQ L I  RD ++    MGIA YN+ CR          IV+RYTKEWE TV R+GRWIDF NDYKTM P FMESVWWVF+T+ EKGLVYRGYK                    VMP+STAC+TPLSNFEAGLNYKDVKDP+IVV+F +  + +ASLLAWT+TPWTLPSNLALCVN +F+Y+K+                                                       RD   GK + L          +K+RL Q+YP M TKKWKPE A ET+EILE +KG+ALVG  Y PLFPYF  R    G+F V+SD YVT+D GTGIVHQAPAFGEDDYRVCL H ++AK E                PCPVD NGRFT+                 DF+GRH+KEAD DICA LK +GRL+ K  Y HSYP+CWRSDTPLIYKAVPSWFV+VE IK+RLL NN+ TYWVPA VKEKRFHNWL                            DAKDWAISRNRFWGTPIPLW S+D +E +A+GSV++L  LSGV+                                                                    + DLHRE IDHITIPSK+G+G LRRVDEVFDCWFESGSMPYAQ HYPFENKERFEAGFPADFIAEGLDQTRGWFYTL V+STALFDKP FKNL+ NGLVLA DGKKMSKRLKNYPDP+                                     V++ YGADALRLYL++SPVVRAEPLRF+E GV AVVKDV LPW+N  RFF+Q                                    ARRWE    + GG  F P   AAA S N  D W++A+ + L++FV QEM AYRLYTVVP L+ F+  LTNWY+RLNR RLKG DG  EA   L CLY+VL  +  +MAP  PFF+E +YQ LR         D        G A SVHY  LP   A      ++A + A  A            L+RA   +EL R AR++RN+SLK PV+ ++VV  D+A L  + +LAGYVR E+N   +  ++DE AWC  +A+ NNK LG+R GK    ++   A+ T+ QL AF A+                             G + + G  L AE+L+VRR F GD A YEA  + DG  VV LDT RD  + Q  TARE +NRVQKLRK                      AGL M D VEVFF E AG T ++AA+AA+  ++  A G+ PLP  A  P
Sbjct:    3 GHYVSRRFGWDCH--------------GLPVEYEIDQMLKITDRDQIVGPEGMGIAAYNAECR---------KIVTRYTKEWEVTVSRMGRWIDFENDYKTMLPWFMESVWWVFKTMFEKGLVYRGYK--------------------VMPYSTACSTPLSNFEAGLNYKDVKDPAIVVSFPVEGE-EASLLAWTTTPWTLPSNLALCVNVDFDYVKV-------------------------------------------------------RDVAGGKVYYL----------AKERLCQLYPQMLTKKWKPEKAGETFEILEELKGAALVGRRYTPLFPYFAARAESHGNFRVVSDGYVTSDSGTGIVHQAPAFGEDDYRVCLEHGLIAKDEE--------------APCPVDFNGRFTEAVA--------------DFQGRHIKEADADICAHLKERGRLVQKAVYDHSYPFCWRSDTPLIYKAVPSWFVSVEKIKERLLANNDQTYWVPAFVKEKRFHNWLR---------------------------DAKDWAISRNRFWGTPIPLWCSDDYQEIVAVGSVDELEALSGVR--------------------------------------------------------------------VEDLHREHIDHITIPSKEGRGALRRVDEVFDCWFESGSMPYAQQHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAGDGKKMSKRLKNYPDPS------------------------------------IVVSKYGADALRLYLVNSPVVRAEPLRFKEEGVLAVVKDVFLPWYNAFRFFMQN-----------------------------------ARRWELTHPEGGGRRFAPDAAAAAGSANPTDAWILAAKEDLIKFVRQEMGAYRLYTVVPELLTFLNHLTNWYIRLNRDRLKGKDGPAEAHAALCCLYDVLFALCLLMAPLTPFFAETLYQHLRPFRPE--AADAGAAEDAPGKAASVHYCTLPAARA---GAPSDAAIGAKMAV-----------LRRA---VELGRVARERRNLSLKHPVRAVVVVCADRAKLDGLRELAGYVRSELNAVTLELTADEDAWCKYTAETNNKALGKRLGKDLRAVRAAAARLTNDQLRAFQAE-----------------------------GALTLEGHALGAEDLVVRREFIGDTARYEADTAPDGSFVVALDTTRDAALEQMGTAREVVNRVQKLRKA---------------------AGLQMEDAVEVFFEEEAGKTAVAAALAANAGLLEGALGAAPLPLGARAP 999          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A7R9Y9K5_9STRA (Isoleucine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9Y9K5_9STRA)

HSP 1 Score: 1096 bits (2834), Expect = 0.000e+0
Identity = 649/1486 (43.67%), Postives = 796/1486 (53.57%), Query Frame = 0

Query:   21 LPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVS--DDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSES---AGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPALSVAR 1501
            LP  INF+ EE++  AFWD+I AF+TS +         LS GR  +TFYDGPPFATGLPHYGH+LAGTIKDTVTRYAHQTG+ V RRFGW CH              GLPVE+EID+ L I SRD +L MG+  YN  CR          IV RY  EW   V+RLGRWIDF NDYKTMD  FME++WWVF  LH K LVY G+K                    VMP+ST C+TPLSNFEA  NYKDVKDP++ V+F++    +A  +AWT+TPWTLPSNLALCVNPE EY+KI                                                          +  K +IL           K RL Q++P M TKKWKPE AAE Y+ILE+  GS L G+ YEP+ PYF++R  SF +L D YVT D GTG+VHQAPAFGEDDYRVCLA+ V+  G                VPCPVD  GRFT+                 DF G++VK+AD  I A LK +G L+ K+ Y H+YP+CWRSDTPLIY+AVPS FV VE +K RL+ NN  T WVP  + + RFHNWL                            DA+DWAISRNRFWGTPIPLW S+D EE +A+ S+ +L ELSG K                                                                    + DLH E+I HITIPS++GKG LRRV EVFDCWFESGSMPYAQ+HYPFEN+  F+ GFPA FIAEGLDQTRGWFYTL VL TALFDKPPF+N++ NGLVLA DGKKMSKRLKNYPDP                                      VI  YGAD LRLYLI+SP VRA PLRF E GV  +V+DV+LPW+N LRF  QQ                                     RWE +  G   F P  E AAAS NV+D W++A LQGL+++VH+EM  YRLYTVVPRLV FI  LTNW+VRLNR RLKG +G +EA VGL  LYEVLL M+ +M PF P+F+E+VYQ+LR LH  Y     A  +  +G A+SVHYLMLPQ +A +L+ +    M  +QAS                  ++L R  RDK  I +K PVK++IVV   +    A+E L  Y+  E+N W++  + D+  WCT  A P+NK+LG+R GK   T+ + I      QL  ++                               G+I+V G  ++A EL+V   F GDA  Y A     +   +VV+LDT +DE +L   TARE +NRVQ++RK                      AGL   D+VE+F  E     G+  ++AAI A+    R    ++PLP     P  A VIA D    +      G   V + + LTRP L+V R
Sbjct:   40 LPGRINFSEEEERTKAFWDDIKAFETSLE---------LSRGRQRYTFYDGPPFATGLPHYGHILAGTIKDTVTRYAHQTGHYVERRFGWDCH--------------GLPVEHEIDKKLEIVSRDQILEMGVEQYNKECRS---------IVQRYVGEWRHLVQRLGRWIDFDNDYKTMDVTFMETIWWVFSQLHAKNLVYYGFK--------------------VMPYSTGCSTPLSNFEASQNYKDVKDPAVTVSFKVVGQENAYFVAWTTTPWTLPSNLALCVNPELEYVKILHK-------------------------------------------------------EHNKEYILC----------KARLAQLFPQMLTKKWKPEKAAELYDILESYTGSQLEGMTYEPILPYFRERANSFRMLCDKYVTDDGGTGVVHQAPAFGEDDYRVCLANGVIEVGTD--------------VPCPVDDAGRFTEEVS--------------DFAGQYVKDADELIIAKLKTEGSLVRKEVYNHNYPFCWRSDTPLIYRAVPSLFVRVEELKQRLMENNTQTRWVPEEIGKNRFHNWLR---------------------------DARDWAISRNRFWGTPIPLWVSDDMEEMVAVSSIAELEELSGEK--------------------------------------------------------------------VEDLHMENIQHITIPSRKGKGTLRRVSEVFDCWFESGSMPYAQIHYPFENEGAFKEGFPAQFIAEGLDQTRGWFYTLMVLGTALFDKPPFQNVIVNGLVLASDGKKMSKRLKNYPDP------------------------------------ALVIGKYGADPLRLYLINSPAVRANPLRFEEDGVMGIVRDVILPWYNALRFLAQQVL-----------------------------------RWEHV--GAKPFVPDREKAAASENVIDTWILAELQGLIKYVHEEMNCYRLYTVVPRLVDFIGQLTNWFVRLNRDRLKGINGDDEAFVGLCVLYEVLLQMAILMGPFTPYFAEFVYQKLRPLHSGYNNSSVAIDA--LGRAESVHYLMLPQFDAAMLHEEYCQHMHTLQAS------------------VDLGRYLRDKSGIVMKTPVKKVIVVVGSEDQKLALEILESYLLAELNAWELEITLDQERWCTYKATPDNKVLGKRLGKAFKTVLKSIKSLPHEQLKKYMVD-----------------------------GSIEVDGHAINAGELMVSLEFSGDAERYVASAGKGEYSQLVVILDTEQDEALLAAGTARECVNRVQRMRKT---------------------AGLEPSDEVEIFLYEEHGGEGAGPVTAAIKANAEYCRSKLRTLPLPMTLANP-FAQVIAVDTCDFELP----GDRKVHSKLMLTRPCLAVQR 1137          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: W2Q337_PHYPN (Isoleucine--tRNA ligase n=29 Tax=Peronosporaceae TaxID=4777 RepID=W2Q337_PHYPN)

HSP 1 Score: 1093 bits (2828), Expect = 0.000e+0
Identity = 634/1457 (43.51%), Postives = 803/1457 (55.11%), Query Frame = 0

Query:   21 LPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEV-GAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHA-PVIASDAFTLD 1475
            LP  I+F  EE K+L  W++++AF  S +         LS+ R  FTFYDGPPFATGLPH+GH+LAGTIKDTVTRYAHQTG+ V RRFGW CH              GLPVE EI++ LG+ +++ VL MGI  YNS CRG         IV RYT EWE  VKR+GRWID  NDYKTM+P +MESVW VFRT+ +K LVYRGYK                    ++P+STAC T LSNFEA L+Y+D  DPS+VV F + D+PD +LLAWT+TPWTLPSNLALCVN  F+Y+KI                                                       +D K+ K +IL           + RL QVYP M  K +K       +EILE  KGS LVG  Y PLF  FKD P +F VLSD+YV+   GTGIVHQAP FGEDDYRVC+   V  K                 +P P+D NG FTD                   KG HVK+AD+ IC  LK +GRL+ K    HSYP+C+RS TPLIY+A+P WFV VE I+DR++ NN+ TYWVP+ V+EKRFHNWL                            D KDW ISR RFWGTP+PLW S+D EE + +GS+ +L EL+G K                                                                    +TDLHRE IDH+TIPSKQGKGVLRRV EVFDCWFESGSMPYAQ HYPFENK++FEA FPADF+AEGLDQTRGWFYTL VLSTALFDKP FKNL+ NGLVLAEDG+KMSK LKN+ DP  +                                    +  YGADALRLYLI+SPVVRAEPL+F+ PGV  V++++ LPWFN+ RFF QQ                L    GV                        AF P+ EAA ASTNVMD W++A++  L++FVH+EM AYRLYTVVPRLV+FI  LTNWYVRLNR RLKGS G  EA V L+ LYEV   ++++MAPF PFF+EY+YQ LR+ H N   G G + + +  G + SVH+LMLP  +A  ++ + E  M  +Q+                  VIE+ R  R++R ISLK PVK++IVV+ +Q  L  + +L  Y+  E+N+  +  S+DE  WC L A+ N++ LGRR GK  + +K+ IAQ T   ++A+++                             +G++ + G  L+ E+L+V+R F+GD+ ++EA VS +G ++V++DTR DE +  Q  ARE + RVQKLRK                      AGL + DK+ V+F E  G   +S AI   + MI    G+ P P +  QP H+ P+++ +A   D
Sbjct:   11 LPERISFPDEELKILELWEQLNAFHKSLE---------LSKDRKPFTFYDGPPFATGLPHHGHILAGTIKDTVTRYAHQTGHYVERRFGWDCH--------------GLPVENEINKKLGVTTKEQVLEMGIDKYNSECRG---------IVQRYTSEWERVVKRIGRWIDCKNDYKTMEPWYMESVWNVFRTIFDKDLVYRGYK--------------------ILPYSTACTTSLSNFEANLDYRDTPDPSVVVNFPLADEPDVALLAWTTTPWTLPSNLALCVNESFDYVKI-------------------------------------------------------KDIKTEKVYIL----------DEARLCQVYPKMSKKGYK----GGEFEILEKFKGSTLVGKKYVPLFDCFKDWPNAFRVLSDNYVSDSGGTGIVHQAPTFGEDDYRVCVREGVADK---------------FTLPDPLDDNGVFTDAVPLV--------------KGLHVKKADDVICQDLKNRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIVANNKLTYWVPSFVQEKRFHNWL---------------------------VDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELEELAGEK--------------------------------------------------------------------VTDLHREFIDHLTIPSKQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKDKFEANFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKNFTDPEEI------------------------------------LQKYGADALRLYLINSPVVRAEPLKFQAPGVLGVIREIFLPWFNSARFFAQQATR-------------LQLETGV------------------------AFVPNREAALASTNVMDSWIIAAMHNLIKFVHEEMKAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEAAVALSALYEVEYNLAKLMAPFTPFFTEYMYQFLRQFHPNVVNGAGKDLAEDADGVSPSVHFLMLPDFDASRVDEEVEELMKNLQS------------------VIEMGRVVRERRTISLKNPVKKVIVVSNNQKALDGLRRLETYLHDELNMRDLEFSTDEKEWCVLKAEANSRALGRRLGKALSGVKKQIAQMTHDDVAAYVS-----------------------------SGSVTIDGHELTGEDLVVKREFKGDSKIFEADVSPEGNLMVIIDTREDEELKMQGCAREVITRVQKLRKK---------------------AGLVVQDKIHVYFEEKGGKGPISTAIQTFLPMISSTLGTAPAPLS-LQPQHSVPIVSEEAQFAD 1080          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A2D4BRB4_PYTIN (Isoleucine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BRB4_PYTIN)

HSP 1 Score: 1093 bits (2827), Expect = 0.000e+0
Identity = 640/1455 (43.99%), Postives = 802/1455 (55.12%), Query Frame = 0

Query:   16 PQVEDLPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSESAGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASD 1470
            P    LP  I+F  EE K+L  WDE++AFQ S +         LS+GR  FTFYDGPPFATGLPH+GH+LAGTIKDTVTR+AHQTG+ V RRFGW CH              GLPVE EI++ L + +++ VL MGI  YN+ CRG         IV RYTKEWE TVKR+GRWID  NDYKTM+P +MESVW VF+T+ EK LVYRGYK                    ++P+STAC T LSNFEA ++Y+DV DPS+VV+F + D+P+ SLLAWT+TPWTLPSNLALCVN  F+Y+KI                                                       +D K  + ++L          ++ RLVQ+YP M  K +K       +EILE  KG+ALVG  Y PLF  F   P +F VLSD+YVT  +GTGIVHQAP FGEDDYRVC+   VV K E               +P P+D NGRFTD                   KG HVKEAD  +C  LK K RLI K Q  HSYP+C+RS TPLIY+AVP WFV VE I+DR++ NN  TYWVP+ VKEKRFHNWL                            D KDW ISR RFWGTP+PLW SED EE + +GS+++L ELSG K                                                                    +TDLHRE IDHITIPS+QGKG LRRV+EVFDCWFESGSMPYAQ HYPFENKE FEA +PADFIAEGLDQTRGWFYTL V+STALFDKP FKNL+ NGLVLAEDG+KMSK L+N+ DP      T++  R                              YGADALRLYLI+SPVVRAEPL+F + GV  V++++ LPW+N++RFF Q                                    A R E  +  G AF PS   A AS NVMD W++A+L  L++FVH EMAAYRLYTVVPRLV FI  LT WYVRLNR RLKGS+G+++A V L+ +YEVL  + ++MAPF PFF+EY+YQ LR+ H N             G A+SVH+LM+P  +A  ++   E                TC  +K    VIE+ R  R++R ISLK PV+++IVV+ D A +  +  L  YV  E+N+  +  ++DE  WC L A+ N+K LGRR GK  + +++ +   +  +++AF+                                +I + G  LS ++L+V R F+GD  +YEA VS +  ++VVLDTR DE++ QQ  ARE + RVQKLRK                      AGL + DK+ VFF E+ G +T++AA+    AM+  A GSVP    A +P H+  I S+
Sbjct:    4 PATRSLPERISFPEEEVKILQLWDELNAFQKSLE---------LSKGRKPFTFYDGPPFATGLPHHGHILAGTIKDTVTRFAHQTGHYVERRFGWDCH--------------GLPVENEINKKLKVTTKEQVLEMGIDKYNAECRG---------IVQRYTKEWERTVKRIGRWIDCENDYKTMEPWYMESVWHVFKTIFEKDLVYRGYK--------------------ILPYSTACTTSLSNFEANMDYRDVSDPSVVVSFPIVDEPEVSLLAWTTTPWTLPSNLALCVNESFDYVKI-------------------------------------------------------KDIKLNRVFVL----------AESRLVQLYPTMEKKGYK----GGEFEILEKFKGTALVGKKYTPLFDCFASWPNAFRVLSDNYVTDGNGTGIVHQAPTFGEDDYRVCVKQGVVDKFE---------------LPNPLDDNGRFTDAVPFV--------------KGLHVKEADPIVCNLLKEKDRLIRKGQIVHSYPFCYRSGTPLIYRAVPGWFVNVEKIRDRIVENNRQTYWVPSFVKEKRFHNWL---------------------------VDGKDWNISRGRFWGTPLPLWVSEDFEEIVCVGSIQELEELSGEK--------------------------------------------------------------------VTDLHREFIDHITIPSRQGKGQLRRVEEVFDCWFESGSMPYAQKHYPFENKEAFEASYPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAEDGRKMSKSLRNFTDP------TEILER------------------------------YGADALRLYLINSPVVRAEPLKFVDSGVMGVIRELFLPWYNSVRFFSQN-----------------------------------ATRLE--ETTGQAFVPSSAEALASKNVMDSWIIAALHNLIKFVHTEMAAYRLYTVVPRLVEFIDQLTKWYVRLNRPRLKGSEGNDDAHVALSAVYEVLYTLCKLMAPFTPFFAEYMYQFLRQYHPNVVNQVAGLAEDADGVARSVHFLMVPDFDASRVDENVE----------------TC--MKNLQCVIEMGRVVRERRTISLKNPVRKVIVVSSDAATVDGLRNLESYVHMELNMRDLEFTTDEKQWCVLKAEANSKALGRRLGKALSGVRKAVDALSHDEVNAFLQNK-----------------------------SITIDGHELSGDDLIVIREFKGDKKIYEANVSLESNLMVVLDTREDEQLKQQGCAREFITRVQKLRKK---------------------AGLEVQDKIHVFFQEADGQSTITAAVKEFAAMVAGALGSVPA-LLALKPAHSVTIISE 1071          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A7S3GN89_9STRA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GN89_9STRA)

HSP 1 Score: 1092 bits (2825), Expect = 0.000e+0
Identity = 615/1272 (48.35%), Postives = 730/1272 (57.39%), Query Frame = 0

Query:   21 LPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDR-PGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFI 1291
            L   I+F AEE+K LA+W EIDAF+TS K         LSE +PE+TFYDGPPFATGLPHYGH+LAGTIKDTVTR+AHQTG+ V RRFGW CH              GLPVEYEIDQ L I  R  VL MG+A YN+ CRG         IV+RYT EWE TV RLGRWIDF NDYKTMDP FMESVWWVF T+ +KGLVYRGYK                    VMP+ST C TPLSNFEAGLNYKDV DP+ VV+F +  DP+ SLLAWT+TPWTLPSNLALCVN +FEY+KI                                                      I  T+  + +IL           + RL Q++P +       E+ A  YE++E + G  LVG+ Y PLF YF    P +F V+SDSYVT + GTGIVHQAPAFGEDDYRVCL +++VAKGE               +PCPVDANG FT                  +F GR VKEADNDICA LK  GRL++K+QY HSYP+CWRS+TPLIYKAVPSWFV+VE IK +LL NN  TYWVPA V+EKRFHNWL+                           DAKDWAISRNRFWGTPIPLW S+D EE + IGSVE+L++LSGV+                                                                    +TDLH+E +DHITIPSKQGKG+L+RVDEVFDCWFESGSMPYAQ+HYPFENKERF  GFPADFIAEGLDQTRGWFYTL V+ST LFDKP FKNL+ NGLVLA DGKKMSKRLKNYP P                                      V+   GADALRLYLI+SPVVRA+ L+F++ GV  VV+ VLLPWFNT RFF Q                C+                    RWE++   G  F P+ E +  STN +D+W++A+  GLV+FVHQEM AYRLYTVVPRLV FI  LT WY+RLNR R+KG    EEALVGL  LYEVL  MSQ+MAPF PFF+EY+YQ LRKLH NY   D A      G + SVHYLMLP  +   LN +A A    +Q +                  + LAR AR++R+I   +P++ + VV  +Q  + A++ L  Y   E+N   V  S++    C L   PN K LG+R GK    + + + + T AQ+  F+
Sbjct:   17 LADKISFPAEEEKTLAYWTEIDAFKTSLK---------LSENKPEYTFYDGPPFATGLPHYGHILAGTIKDTVTRFAHQTGHHVSRRFGWDCH--------------GLPVEYEIDQKLKITGRQMVLDMGVANYNAECRG---------IVTRYTAEWEKTVNRLGRWIDFKNDYKTMDPEFMESVWWVFDTMFKKGLVYRGYK--------------------VMPYSTYCGTPLSNFEAGLNYKDVNDPAAVVSFPLESDPNVSLLAWTTTPWTLPSNLALCVNEKFEYVKI------------------------------------------------------IDKTRGNRVFILL----------EKRLGQIFPEVNKPNCTDEIRATLYEVVERMTGDKLVGLKYTPLFNYFVATCPQAFRVVSDSYVTEEGGTGIVHQAPAFGEDDYRVCLKNDIVAKGED--------------LPCPVDANGLFT--------------AEVPEFVGRGVKEADNDICALLKNNGRLVLKEQYAHSYPFCWRSETPLIYKAVPSWFVSVEKIKPQLLANNSLTYWVPAFVQEKRFHNWLA---------------------------DAKDWAISRNRFWGTPIPLWISDDLEEIVTIGSVEELYQLSGVR--------------------------------------------------------------------VTDLHKEHVDHITIPSKQGKGLLKRVDEVFDCWFESGSMPYAQMHYPFENKERFTNGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYPAPE------------------------------------IVVNECGADALRLYLINSPVVRADVLKFQQEGVVEVVRGVLLPWFNTFRFFTQ----------------CVD-------------------RWESIS--GTKFVPNVEVSKGSTNDVDVWILAATVGLVEFVHQEMKAYRLYTVVPRLVDFIDQLTKWYIRLNRFRIKGESSQEEALVGLNVLYEVLATMSQIMAPFTPFFTEYLYQHLRKLHPNYGSTDPAVPVDSFGRSDSVHYLMLPVPDESRLNARAVARFNTLQQA------------------VTLARIARERRHIRNNLPLRSVTVVAANQEDVEALQYLHTYFVSEINALDVQLSTEWEKMCLLKVQPNWKDLGKRLGKQMKDVAKAVNELTTAQIVDFM 958          

BLAST of jgi.p|Trimin1|346771 vs. uniprot
Match: A0A5D6XPJ1_9STRA (Isoleucine--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XPJ1_9STRA)

HSP 1 Score: 1091 bits (2821), Expect = 0.000e+0
Identity = 641/1481 (43.28%), Postives = 801/1481 (54.09%), Query Frame = 0

Query:   21 LPANINFAAEEDKVLAFWDEIDAFQTSFKALFLLLPNALSEGRPEFTFYDGPPFATGLPHYGHLLAGTIKDTVTRYAHQTGYRVRRRFGWHCHXXXXXXXXXXXXXXGLPVEYEIDQTLGIKSRDDVLAMGIATYNSHCRGAPXXXXXXXIVSRYTKEWEGTVKRLGRWIDFANDYKTMDPAFMESVWWVFRTLHEKGLVYRGYKSXXXXXXXXXXXXXXXXXXXVMPFSTACATPLSNFEAGLNYKDVKDPSIVVAFQMRDDPDASLLAWTSTPWTLPSNLALCVNPEFEYIKISXXXXXXXXXXXXXKISTPCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIRDTKSGKAWILXXXXXXXXXXSKDRLVQVYPIMGTKKWKPEMAAETYEILETIKGSALVGIAYEPLFPYFKDRPGSFTVLSDSYVTADDGTGIVHQAPAFGEDDYRVCLAHEVVAKGEXXXXXXXXXXXXXXXVPCPVDANGRFTDXXXXXXXXXXXXXXXXXDFKGRHVKEADNDICAALKAKGRLIIKDQYTHSYPYCWRSDTPLIYKAVPSWFVAVESIKDRLLVNNEGTYWVPANVKEKRFHNWLSNAXXXXXXXXXXXXXXXXXXXXXXXXXDAKDWAISRNRFWGTPIPLWTSEDGEERIAIGSVEQLHELSGVKLHELXGVKELIXXXXXXXXXXXXXXXMSVDSXXXXLQQLSGIEARTALQGELRAEHGLARLRELYGVHITDLHRESIDHITIPSKQGKGVLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLSVLSTALFDKPPFKNLVCNGLVLAEDGKKMSKRLKNYPDPNNVSVPTQVANRWQMDVDGNVMSXXXXXXXXXXXXXXXVITAYGADALRLYLIDSPVVRAEPLRFREPGVQAVVKDVLLPWFNTLRFFVQQXXXXXXXXXXXXXXXCLSSRRGVGRHYTPCGSLLIARRWEALDAGGAAFKPSPEAAAASTNVMDIWVMASLQGLVQFVHQEMAAYRLYTVVPRLVAFIKDLTNWYVRLNRSRLKGSDGHEEALVGLACLYEVLLCMSQVMAPFMPFFSEYVYQRLRKLHHNYTPGDGANGSSEVGAAQSVHYLMLPQHNAKLLNRQAEADMAAMQASFLPARTMTCSNLKRANIVIELARTARDKRNISLKMPVKEMIVVTKDQAVLSAVEKLAGYVRGEMNVWQVTTSSDEGAWCTLSADPNNKLLGRRFGKGAATIKEGIAQPTDAQLSAFIAQAPRPNPCAXXXXXXXXXXXXXXXXXXXATGTIDVAGATLSAEELLVRRAFRGDAAVYEACVSDDGGVVVVLDTRRDERVLQQRTAREALNRVQKLRKXXXXXXXXXXXXXXXXXXXXXXAGLNMGDKVEVFFSES----AGSTTLSAAIAAHVAMIRDATGSVPLPAAAFQPPHAPVIASDAFTLDADDGSCGAPAVTATITLTRPAL 1497
            LP  I+F  EE K+L  W+E+DAF+ S          ALSEGR  FTFYDGPPFATGLPH GH+LAGTIKDTVTRYAHQTG+ V RRFGW CH              GLPVE EI++ L + +++ V  MGIA YN+ CRG         IV RYT EWE TVKR+GRWID  NDYKTM+P +MESVW VF+T+  K LVYRGYK                    ++P+STAC T LSNFEA ++Y+D  DPS+VV+F + D+PD +LLAWT+TPWTLP+NLALCVN  F+Y+K+                                                       RD KSGK ++L          ++ RLVQVYP +  K +K       +E+L T KG+ LVG  Y PLF  F   P +F VLSD+YV+   GTGIVHQAP FGEDDYRVC+   V  K                 +P  +D NGRFTD                    G HVK+AD+ IC  LKA+ RL+ K    HSYP+C+RS TPLIY+A+P WFV VE I+DR++ NN+ TYWVPA V+EKRFHNWL                            D KDW ISR RFWGTP+PLW S+D EE + +GS+ +L  LSG K                                                                    +TDLHRE IDHIT+PS QGKGVLRRV EVFDCWFESGSMPYAQ HYPFENK++FE GFPADF+AEGLDQTRGWFYTL VLSTALFDKP FKNL+CNGLVLAEDG+KMSK L+NY DPN +                                    +  YGADALRLYLI+SPVVRAEPLRF++ GV  V++++ LPWFN  RFF Q                                    AR   +  AG A F PS  AA ASTN MD W++A+L  L++FVH+EM AYRLYTVVPRLV FI  LT WYVRLNR RLKGS G+ EA V L+ LYEVL  ++++MAPF PF +EY+YQ LR+ H +             G A+SVHYLMLP  +A  ++   EA M  +Q                   VIE+ R  R++R ISLK PV +++VV+ D+A L  +E L  Y+  E+N+  +  S+DE  WC L AD NNK LG+R GK  +++++ +A  +   ++AF+A                             T  + V G  L  ++++V+R F+GD AVYEA VS  G ++VVLDTR D ++  Q  ARE + RVQKLRK                      AGL   DK++V+F+E       S  ++AA+ A VA I  + GS P P A   P   P++  +A   D+            +I +TRPA+
Sbjct:   10 LPERISFPDEEHKILRLWEELDAFKKSL---------ALSEGRKPFTFYDGPPFATGLPHNGHILAGTIKDTVTRYAHQTGHYVERRFGWDCH--------------GLPVENEINKKLNVTTKEQVFEMGIAAYNAECRG---------IVQRYTAEWERTVKRIGRWIDCENDYKTMEPWYMESVWNVFQTIFNKDLVYRGYK--------------------ILPYSTACTTSLSNFEANMDYRDTPDPSVVVSFPLVDEPDVALLAWTTTPWTLPANLALCVNAAFDYVKV-------------------------------------------------------RDLKSGKLFVL----------AQTRLVQVYPKLDKKGYK----GGEFEVLATFKGAELVGKQYAPLFDCFATWPNAFRVLSDAYVSDTGGTGIVHQAPTFGEDDYRVCVQQGVTDK---------------FNLPATLDDNGRFTDAVPLVA--------------GMHVKDADDVICKELKARDRLVSKGTIVHSYPFCYRSGTPLIYRAIPGWFVNVEKIRDRIVANNKATYWVPAFVQEKRFHNWL---------------------------VDGKDWNISRGRFWGTPLPLWVSDDYEEIVCVGSIAELEALSGEK--------------------------------------------------------------------VTDLHREFIDHITLPSAQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKDKFEQGFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLICNGLVLAEDGRKMSKSLRNYTDPNEI------------------------------------LDKYGADALRLYLINSPVVRAEPLRFQDAGVLGVIRELFLPWFNAARFFAQN----------------------------------AARLQASTGAGDAPFVPSQAAALASTNDMDAWILAALHNLIKFVHEEMRAYRLYTVVPRLVGFIDQLTKWYVRLNRLRLKGSAGNAEAHVALSALYEVLYTLAKLMAPFTPFLAEYMYQFLRQFHPSVVNAVEGLAEDADGVAKSVHYLMLPDFDAARIDESVEARMQNLQD------------------VIEMGRVVRERRAISLKNPVAKVLVVSADRAALDGLELLKTYLHDELNMRDLEFSTDEKEWCVLKADVNNKALGKRLGKALSSVRKQVAALSHDDVAAFLA-----------------------------TQRMTVDGHVLEGDDVVVKREFKGDRAVYEADVSASGSLLVVLDTREDAQLKLQGCAREFITRVQKLRKK---------------------AGLEPHDKIQVYFAEKETGGGASGPITAAVHACVATIAASLGSAPAPLALLPPHGVPIVEEEAQFADSS----------VSIVVTRPAV 1097