mRNA_7995 (mRNA) Tribonema minus UTEX_B_3156

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A835YK17_9STRA (Subtilisin (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YK17_9STRA)

HSP 1 Score: 1412 bits (3654), Expect = 0.000e+0
Identity = 773/773 (100.00%), Postives = 773/773 (100.00%), Query Frame = 1

Query:    1 HDRVVIRGVDALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVGYQPGLLSYADTAMYVSARFTVAGSSPQEATTANYAGAFAAALAAGFAAXXXXXAADVALVRVDTVAGAADAAAVSLRVTVADITAGGAVLAYLLAVSEDTGGENAALVARLVSALSGVTSDGCVCATTATAGTYDCARCAAGQPYGTVTAVTLAAGDYDLFTAAADASGLSGSANSAKIRSPILDGIVRTSSYVYIGAVLGAIAVASGVAGLLILCVGR 2319
            HDRVVIRGVDALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVGYQPGLLSYADTAMYVSARFTVAGSSPQEATTANYAGAFAAALAAGFAAXXXXXAADVALVRVDTVAGAADAAAVSLRVTVADITAGGAVLAYLLAVSEDTGGENAALVARLVSALSGVTSDGCVCATTATAGTYDCARCAAGQPYGTVTAVTLAAGDYDLFTAAADASGLSGSANSAKIRSPILDGIVRTSSYVYIGAVLGAIAVASGVAGLLILCVGR
Sbjct:    1 HDRVVIRGVDALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVGYQPGLLSYADTAMYVSARFTVAGSSPQEATTANYAGAFAAALAAGFAAXXXXXAADVALVRVDTVAGAADAAAVSLRVTVADITAGGAVLAYLLAVSEDTGGENAALVARLVSALSGVTSDGCVCATTATAGTYDCARCAAGQPYGTVTAVTLAAGDYDLFTAAADASGLSGSANSAKIRSPILDGIVRTSSYVYIGAVLGAIAVASGVAGLLILCVGR 773          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A835Z5P2_9STRA (Subtilisin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z5P2_9STRA)

HSP 1 Score: 632 bits (1629), Expect = 1.250e-206
Identity = 419/835 (50.18%), Postives = 488/835 (58.44%), Query Frame = 1

Query:    1 HDRVVIRGVDALSSSGSALARRHCLLSLTAFLLTSDA-----------------VCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDN----------IACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPY-AAGARVSSNSWGAARDS----------YTTLDAQLDAWAYARPDAVVVVAA------------------------------------GNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQA-----------NPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTH-SVFHPNGGPGADGVNTVERVTVPD----AQAYASFAVTVTASRVAT----PTQPPFFALVVTGGQVVGYQPGLLS----YADTAMYVSARFTVAGSSPQEATTANYAGAFAAALAAGFAAXXXXXAADVALVRVD-----TVAGAADAAA-----------------------------------------------VSLRVTVADITAGGAVLAYLLAVSEDTGGENAALVARLVSALSGVTSDGCVCATTATA 2055
            HDRV +RGVD L+   S  A                                  V YVD LPA+ A NTVAAWI QS   GA P+W+AGL+   E+IA++DSGVD ASCFF+EE               + CS +T+PVTDL +RK+VQYVG+ DC D   GHGTHVAGTLAGAQ  A    G+HLGDG+AF AKLAVFD G + G L TP+DL AQML   Y  AGAR+S+NSWGA              YTTLD Q+D WAY  PDAV+VVAA                                    GNCGD  SGCAG  G  +AGAGSV+SPALAKNVIAVGASES   SA LGG V NVAYFSSQGP  GDGRIKPD++APGYHT SA+A                  CRVAA AGTSMA PVV+AAAA++RQYF+QGWYPSG PNPSD++NPSGALVKA+LVN A PM  YRG A +V LG GPDGYQGWGR+ LDAAL++ GSVD+H  D VY+     V TY++ V   Y GDFRATMVFVEP GAVGA SPVLNDLDL+VTA+D     +VF+PNGG   D VNTVERV V      A+A+ +F VTV A+ + T    P QPPFFAL VTGG+  GY     +    Y+D   YV    +V G++PQEA TAN+  A AAALAA +       AA V+L RVD     T A AADA                                                 V+L   VAD T G A+++YLLAVSE   G  A L+ +L SAL GVT+ GCVC   A A
Sbjct:  239 HDRVTVRGVDRLARVRSGAAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXAARGDVLYVDALPALRAHNTVAAWIAQSARRGATPLWSAGLRGGGEVIAISDSGVDAASCFFTEEPRXXXXXXXXXXXXAVQCSNFTSPVTDLRRRKIVQYVGFADCADAPGGHGTHVAGTLAGAQAGATA--GRHLGDGIAFDAKLAVFDLGASDGTLLTPADLKAQMLTPSYKGAGARISNNSWGAIXXXXXXXXXXXXMYTTLDRQMDMWAYNNPDAVLVVAATFPTPSQYPHPRXXXXXXXXXXXXXXXXXXXXXXXAGNCGDKGSGCAGGAGQ-LAGAGSVMSPALAKNVIAVGASESALGSAALGGDVDNVAYFSSQGPTPGDGRIKPDILAPGYHTVSAEAAXXXXXXXXXXXXXXXXXCRVAAMAGTSMAAPVVSAAAALMRQYFVQGWYPSGRPNPSDVYNPSGALVKALLVNGAAPMARYRGRAESVTLGAGPDGYQGWGRIQLDAALDIGGSVDLHVVDRVYVRAANFVATYSYTVRDGYDGDFRATMVFVEPPGAVGAVSPVLNDLDLIVTAYDSAARPTVFYPNGGAAPDAVNTVERVVVKRDTAAARAFVAFDVTVRAAALVTASASPPQPPFFALAVTGGRARGYDAAAAATRFDYSDATWYVRLTLSVEGTAPQEAATANFGRALAAALAAAYGGGLS--AAAVSLTRVDGVTPSTAAAAADAGKATPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRVRVTLDAQVADATEGAALMSYLLAVSERRDGAAAELLRQLNSALGGVTTGGCVCVARAAA 1068          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: D7FS58_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FS58_ECTSI)

HSP 1 Score: 580 bits (1495), Expect = 5.900e-191
Identity = 373/786 (47.46%), Postives = 487/786 (61.96%), Query Frame = 1

Query:    1 HDRVVIRGVDALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAVD----AAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVGYQPGLLSYADTAM--YVSARFTVAGSSPQEATTANYAGAFAAALAAGFAAXXXXXAADVALVRVDTVAGAADAAAVSLRVTVADITAGGAVLAYLLAVSEDTGGE----NAALVARLVSALSGVTSDGCVCATTAT--AGTYDCARCAAGQPYGTVTAVT-LAAGDYDLFTAA-----------ADASGLSGSANSAKIRSPI-----LDGIVRTSSYVYIGAVLGAI 2271
            +DRVV+RG D+L ++    A  HCLL++ A+L+T   V YVD LP V  LN  AAWI QSG E AY MW  G+   TEIIA+ADSG+D+ SCFF+E+   DNI CS+++NPV DL KRK+VQYVG+VDC D + GHGTHVAG+ AG   +++  PG+H GDG  F AKLAVFDFG++ G L TPS L +QML  PY  GAR+S+NSWGA R +YT+LD Q+D +AY   D V+V+AAGNCGD  SGC     A I G+GS+LSPAL KN+I+VGASE++ ++A L   V  VAYFSSQGP   D RIKPDVVAPGYH FSA A P +  SC +   AGTSMATPVVA AAAMIRQYF+QGWYPSG   PS+I NPS ALVKA++V +   M +Y   A +  LG+ PD  QG+GRVLLD+ LNV GSVD+  +D V M   G V ++   +D    +A+ GD + TMV++EPAG  G+ +PV NDLDL V A+DG + S F+PN     D  NTVE V +PD  +Y  F VTVTA+ V T T  P++ALVVTGG    Y P     A TA   +V     + G    +  T  +    A A++A         ++ V  +   T + + D + V++ + VA    G AVL Y+++  E T       N  LVA L  AL   T++GC C        G YDC+ C  G  + T +++T ++ G YDL +             A AS  S + +     S +     + GI+R+ SYV++  V+ AI
Sbjct:  176 NDRVVLRGADSLGNTPEDNA--HCLLTVLAYLVTRPEVSYVDDLPQVFELNVEAAWITQSGEETAYSMWNEGIDGSTEIIAMADSGLDVNSCFFTEDLTDDNIDCSSWSNPVFDLTKRKIVQYVGFVDCVDTENGHGTHVAGSAAGGLSNSS--PGEHFGDGAGFGAKLAVFDFGNSEGGLVTPSSLKSQMLQPPYDIGARISTNSWGAVRTTYTSLDRQVDDFAYLNQDMVIVIAAGNCGDEISGCQYQN-ADIYGSGSILSPALGKNIISVGASEASPSTAGLADNVDEVAYFSSQGPTVDDNRIKPDVVAPGYHLFSASAKPDQESSCEIGPMAGTSMATPVVAGAAAMIRQYFVQGWYPSGIATPSNIFNPSNALVKAMIVTSGTAMASYDSSAGSTTLGSPPDAIQGFGRVLLDSVLNVRGSVDLFVEDRVDMT-HGDVNSHFIEMDGVSSSAFDGDMKVTMVWMEPAGFSGSVAPVFNDLDLKVVAYDGTSESTFYPNNLAAKDPDNTVEMVVIPDVDSYEWFNVTVTAASVTT-TSLPYYALVVTGGVAGTYSPT----ASTARSWFVRWEMDIDGIPVAQVATEAFEAGLAEAISAETGDYTDETSSVVVKIDHSTASASDDRSTVTVEIVVASEADGYAVLTYIISFVEGTSSSVAEGNQGLVAALNEALESRTTEGCACVEVQAIDGGGYDCSACEEGAVFSTASSITTMSPGGYDLLSVDRSSGRYPVSVFASASDNSDADDDGMFWSDLSGGKTIRGIIRSDSYVHMAGVVAAI 950          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: D7FS20_ECTSI (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FS20_ECTSI)

HSP 1 Score: 325 bits (834), Expect = 6.580e-92
Identity = 226/505 (44.75%), Postives = 303/505 (60.00%), Query Frame = 1

Query:   70 CLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAG--ALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAG-AGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGL-----AATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTYTFAV--DAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPD 1554
            CL +L AF      V YV         N  AAW+ QS V+G  P+W   LQ   EII VAD+G+DL  C F E+D  DNI  S + +PVTDL KRKVVQY+ +VD  DE  GHGTHVAG++ GAQ       G    DG+AF  KLAVFDFGD+    AL TP ++ + +L   Y  GARV SNSW      YT L  ++D + Y   + +VV AAGNCGD ++    D  + +    G+VLSPA  KNV+AVG+SES G +   G  +  V+YFSS+GP   DGRIKPDVVAPG  T+SA A+     +C   ++ GTSM+TP+VA   AM RQYF +GWY +G+ N S   +PSGAL+KAVL+N+A  M+ + G+        V L T PD +QG+GRV +  ALN+  +V +  +D V +   G    Y  ++  DA    D R T+V+ +P  +VG+   +++DLDL+V A    T + ++PNG   AD VN VE++T+ D
Sbjct:  384 CLSALLAFFSLQPEVRYVTARRKTATQNLDAAWVTQSAVDGYTPLWDENLQGQDEIIGVADTGLDLNHCQFREDDG-DNIEASDWDDPVTDLDKRKVVQYIDFVDDFDEADGHGTHVAGSICGAQSGEDA--GDDDADGMAFQGKLAVFDFGDSDNFNALTTPDEIDSVILEPAYDGGARVHSNSWDTVSTEYTALSNEVDEFVYTHQNMLVVFAAGNCGDTSTDDLIDNCSVLDDDEGTVLSPAQGKNVVAVGSSESGGVT---GKDMDTVSYFSSKGPTI-DGRIKPDVVAPGDPTWSAAADTTGE-TCEFGSQTGTSMSTPIVAGNGAMARQYFREGWYNTGSKNESLGFDPSGALLKAVLINSATGMS-FAGVNPDSGTLDVTLTTPPDTHQGFGRVTMINALNINSTVGIFFEDWVEIR-EGETIDYDVSLYEDADTDKDLRITLVWTDPPASVGSTEALIHDLDLVVIAES--TGTEYYPNGLEEADTVNNVEKITITD 876          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A4D9D260_9STRA (Subtilisin n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D260_9STRA)

HSP 1 Score: 315 bits (807), Expect = 7.950e-89
Identity = 224/538 (41.64%), Postives = 315/538 (58.55%), Query Frame = 1

Query:   70 CLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPG-SCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAY-RGLAATVPLGTGPDGYQGWGRVLLDAALNVA--GSVDMHAQDMVYMNVTGMVKTYTFAVDAAYAGD---FRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGA-DGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVV 1659
            CL SL A+  +   V +++  P V   + +AA  VQSG + + P+W AGL    +II VAD+GVD+ SCFFS  DPT N+A + +   + DL++RKVVQ V   D  D  AGHGTH AGT  G   SA  +       G+A+ AKLA +D G A G L  P+ +  +M A  Y AGARV SNSWG+ +  YT +D + D + Y++PDA++VV+AGN G               G+G+V SP+L+KN + VGAS+S   S+     V N+ +FS QGP   DGRIKPD+VAPG    SA ++  +P  +C V +K+GTSMAT VVA + A++RQ+FL+G YPSGAPNP D   P+GAL+KA+L+N+AVPM  Y +    ++ LG  PD YQG+GR+ LD  L +     V +  +D V +       TY F V          F  T+ + +P     A + VL+DLDL ++ + G T   ++PNG     D +N+VE+V +        + VTVT + ++T TQ   ++L V G  VV
Sbjct:  330 CLTSLVAYFASHPEVIFLETFPIVKPSDAIAAATVQSGEQTSTPLWDAGLDGSGQIIQVADTGVDMTSCFFS--DPTGNVAPTRWDAGMYDLSRRKVVQLVVGEDAFDTPAGHGTHCAGTALG--NSATPSRFNLNFRGVAYMAKLAFYDGGTANGGLKFPASMQMKMFAPGYHAGARVFSNSWGSNQPKYTYMDTEFDGFMYSQPDALIVVSAGNLG-------------ARGSGTVFSPSLSKNALCVGASQSLRRSSYR---VENIPHFSGQGPTF-DGRIKPDLVAPGAFLTSANSSSGDPRYACSVTSKSGTSMATAVVAGSVALLRQFFLEGRYPSGAPNPPDAFVPTGALLKALLINSAVPMERYIQPNGQSIALGLPPDNYQGFGRLQLDIFLPLKPQNPVILFVRDAVPL-AEEETHTYKFTVPDTRTSTLKPFHVTLTWTDPTVPASAGAIVLHDLDLRLSRWLGSTVETYYPNGLTNTPDRINSVEKVVLYAPIPGDVYTVTVTGTSIST-TQYQNYSLAVAGANVV 844          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: K8ZC45_NANGC (Subtilisin n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=K8ZC45_NANGC)

HSP 1 Score: 297 bits (760), Expect = 9.090e-84
Identity = 220/557 (39.50%), Postives = 308/557 (55.30%), Query Frame = 1

Query:   31 ALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAY-------PMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGA-ARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATV-PLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVK----TYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVG 1662
            A  S+ +   +  CLL+L AF+ +  AV YV+   +V   N  AA + QSG +G Y       P++  GL    +++ V D+GVD  SCFFS  D    +  +T +    D ++RK+VQYV + D  D + GHGTHVAGT+AG     +     +  +G+A  AKLA FD G ++G L  PS+ A ++    YAAGAR+ SNSWG  +   YT+ D ++D + Y   D +V+V+ GN G               G G V SPAL+KNV++VGAS S    A L     +VAYFSS GP   DGRIKPD+V PG    SA    A   +C V  K GTSMA PV A +AAM+RQYF++GWYP G    +D   P+GAL+KA+L+N+AVPM A+     T+ PLG  PD YQG GR+ L   L +         ++  +N   + +     Y F++ A      + T+V+++P     +A+ VL+DLDL+V  +     S + PNG   AD VNTVE+V +       +  VTVTA+ VAT  +  + AL  +G  V G
Sbjct:  358 AYDSNSTISDQSACLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAHLTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVDQNSCFFS--DAKGPLPTTTISEAAFDSSRRKIVQYVQWADSTDVENGHGTHVAGTMAGVSLDTSMDMSSY--EGVARGAKLAFFDMGFSSGGLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKGRE-------------GIGGVASPALSKNVLSVGASLSN-RYADL--DPTHVAYFSSWGPTK-DGRIKPDIVTPGRRLVSA----ASGATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAADGFVPTGALLKAMLINSAVPMAAFDFNNGTLLPLGLPPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKFSIPATGKSPVKITLVWIDPDTTASSATTVLHDLDLMV--YSQKEGSSYFPNGRTNADNVNTVEKVALYSPTPGDTLTVTVTATSVATRPEQRY-ALAASGNFVSG 886          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A4D9CUI1_9STRA (Subtilisin n=2 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CUI1_9STRA)

HSP 1 Score: 294 bits (753), Expect = 1.470e-81
Identity = 219/557 (39.32%), Postives = 307/557 (55.12%), Query Frame = 1

Query:   31 ALSSSGSALARRHCLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAY-------PMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGA-ARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHNPSGALVKAVLVNAAVPMTAYRGLAATV-PLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVK----TYTFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTGGQVVG 1662
            A  S+ +   +  CLL+L AF+ +  AV YV+   +V   N  AA + QSG +G Y       P++  GL    +++ V D+GVD  SCFFS  D    +  +T +    D ++RK+VQYV + D  D + GHGTHVAGT+AG     +     +  +G+A  AKLA FD G ++G L  PS+ A ++    YAAGAR+ SNSWG  +   YT+ D ++D + Y   D +V+V+ GN G               G G V SPAL+KNV++VGAS S    A L     +VAYFSS GP   DGRIKPD+V PG    SA    A   +C V  K GTSMA PV A +AAM+RQYF++GWYP G    +D   P+GAL+KA+L+N+AVPM A+     T+ PLG  PD YQG GR+ L   L +         ++  +N   + +     Y  ++ A      + T+V+++P     +A+ VL+DLDL+V  +     S + PNG   AD VNTVE+V +       +  VTVTA+ VAT  +  + AL  +G  V G
Sbjct:  359 AYDSNSTISDQSACLLALLAFVSSDPAVLYVEEYSSVVLYNAAAAHLTQSGDDGQYNQATSSFPLYDVGLDGTNQVVQVVDTGVDQNSCFFS--DAKGPLPTTTISEAAFDSSRRKIVQYVQWADSTDVENGHGTHVAGTVAGVSLDTSMDMSSY--EGVARGAKLAFFDMGFSSGGLTLPSNFATRLFPPGYAAGARIFSNSWGTPSAKFYTSADTEIDKFMYEYDDTLVLVSGGNKGGE-------------GIGGVASPALSKNVLSVGASLSD-RYADL--DPTHVAYFSSWGPTK-DGRIKPDIVTPGRRLVSA----ASGATCGVTIKQGTSMACPVAAGSAAMVRQYFVEGWYPRGVKRAADGFVPTGALLKAMLINSAVPMAAFDFNNGTLLPLGLPPDVYQGHGRIKLADVLPIKSLGATTTPNLYVVNGASIKEEEKHVYKLSIPATGKSPVKITLVWIDPDTRASSATTVLHDLDLMV--YSQKEGSSYFPNGRTNADNVNTVEKVALYSPMPGDTLTVTVTATSVATRPEQRY-ALAASGNFVSG 887          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A836CCI7_9STRA (Subtilisin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CCI7_9STRA)

HSP 1 Score: 258 bits (659), Expect = 3.380e-70
Identity = 218/570 (38.25%), Postives = 289/570 (50.70%), Query Frame = 1

Query:  169 IVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLA-------SCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFG--DAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGS--VLSPALAKNVIAVGASESTGASAPLGGGVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQAN-PAEPGSCRVAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSDIHN----PSGALVKAVLVNAA----------------------------------------------------------VPMTAYRGLAATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVTGMVKTY--TFAVDAAYAGDFRATMVFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVA-TPTQPPFFALVVTG 1647
            + QSG    +PMW+AG+    E+I +ADSGVD          C F + D    +  S +  PVTD  +RK++QY+ YVD  DE+ GHGTHVAGTL GA+       G  + DG AF  K+ VFDFG  D   +L  P D+   +LA  Y AGARV SNSWG    + + +D ++D +       ++V+AAGNCGD  S  + D G    G  S  V SPA AKNV+AVGAS +   +   GG +  +A+FS++GP   DGRIKPDVV PGY   SA AN P    SC  A++ GTSMATPVVAA+AA++RQYF +GWY SG    +D  N    PS AL+KAVLVN+A                                                           P  + R L  T   G  PD  QG GR+LL+  +NV  +V +  +D   +   G V  Y  T    A        T+V+ +P  A GAA PVL+ L + V    G T   + PNG   AD VN V++V +P  +    + + V+A  V  +PTQ  ++AL   G
Sbjct:  305 VAQSGSLNLWPMWSAGILGAGEVIGMADSGVDSPVVSVDRRHCSFYDSDGII-VKASAWNAPVTDNTRRKIIQYIAYVDSEDEKNGHGTHVAGTLVGARDGTTA--GSTITDGQAFEGKITVFDFGASDGTDSLSVPGDVQTMLLAPSYNAGARVHSNSWG----TQSQIDVEMDTYVNDHQQMLIVIAAGNCGDV-SNTSLDCGPEFTGPNSPSVFSPAQAKNVVAVGASYNYPNNVD-GGDINTIAFFSARGPTV-DGRIKPDVVGPGYSLTSASANTPGGGPSCASASRQGTSMATPVVAASAALVRQYFREGWYNSGG-QAADEENFGFLPSAALLKAVLVNSAHAVNNVEQKPANARXXXXXXXXXXXXXXLKWKXXXXXXXXXERALSGDVAQAFGIGSYAPRGSARSLGLTALPGP-PDKAQGHGRILLNMGVNVDATVGLFFKDWQQI-AEGQVVEYNVTTVPTAVTTRLLSVTLVWTDPPSAPGAAKPVLHTLGVRVRE-QGSTVWTY-PNGLAQADDVNNVQKVVLPSTKQGTMYIIEVSAPVVTESPTQ--YYALAAAG 857          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A7S2JW00_9STRA (Subtilisin n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2JW00_9STRA)

HSP 1 Score: 251 bits (642), Expect = 2.660e-68
Identity = 196/548 (35.77%), Postives = 294/548 (53.65%), Query Frame = 1

Query:   70 CLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGG-GVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCR-----------------VAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSD-IHNPSGALVKAVLVNAAVPMTAYRGL-AATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVT-GMVKTYTFAVDAAYAGDFRATM-VFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTG 1647
            C+LSL A +     VC V+ +P +  +N +A WI QSGV+ + P W + L    +II V+DSG+D   C F +  P +    +   N      +RKV++Y  Y D GD   GHGTHV G++ G +            DG+A  AK++ FD G      Y P      + +  Y+AG++  S SWGA   +Y +     D +AY   D +  VAAGN G          G A+   G+V +PA AKN+I+VGASES G     G  G+  +AYFSS+GP + DGR KPD+V+PG+   SA++ P++ G C                  V+ K+GTSMATPV +  AA++RQYF +G+YP+G       I NPS +L+KAV++N A  +    G    ++      D +QG+GR+ L  +L + G+ D   +    ++++ G   +Y++ +    + D  + M V+ +PAG+ G A  ++NDLD L    DG T   ++PNG   AD +N  ER+ +P      + +VTVT + +AT +Q   +ALVVTG
Sbjct:  314 CVLSLVAAIAAQPEVCSVESVPMMKPMNDIAQWITQSGVQNSRPFWDSDLTGAGQIIQVSDSGLDTNHCSFWDSSPGEKRDGTHQPN------RRKVIKYEDYKDDGDYSNGHGTHVVGSIIGHKAKDGVNESSGFIDGMAKDAKVSFFDIGLGTACCYVPGQTT--LFSGGYSAGSKFHSASWGAQTSTYNSNSRGFDNFAYNNKDFLPFVAAGNSGS---------GNAL---GTVGAPATAKNIISVGASESDGRDLYGGSDGLNYLAYFSSRGPTS-DGRRKPDIVSPGHAILSAKSVPSQSGECEPDNASQLPNAGGSSSYAVSYKSGTSMATPVASGTAALVRQYFTEGYYPNGIKGAGTAIANPSASLIKAVMLNGAQSLIGKSGSQGGSISSVAYYDEHQGFGRIQLSTSLPLDGTNDFALEANEKVSISQGDEDSYSYQIGECSSMDELSIMLVWTDPAGSSGCAKCLINDLD-LTAELDGTT---YYPNGRNSADTLNNAERIRLPVTPGD-TVSVTVTGTNLATSSQE--YALVVTG 833          

BLAST of jgi.p|Trimin1|154678 vs. uniprot
Match: A0A7S2NT32_9STRA (Subtilisin n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2NT32_9STRA)

HSP 1 Score: 252 bits (644), Expect = 1.420e-67
Identity = 196/548 (35.77%), Postives = 294/548 (53.65%), Query Frame = 1

Query:   70 CLLSLTAFLLTSDAVCYVDVLPAVTALNTVAAWIVQSGVEGAYPMWTAGLQADTEIIAVADSGVDLASCFFSEEDPTDNIACSTYTNPVTDLAKRKVVQYVGYVDCGDEQAGHGTHVAGTLAGAQRSAAGAPGQHLGDGLAFAAKLAVFDFGDAAGALYTPSDLAAQMLAAPYAAGARVSSNSWGAARDSYTTLDAQLDAWAYARPDAVVVVAAGNCGDAASGCAGDGGAAIAGAGSVLSPALAKNVIAVGASESTGASAPLGG-GVGNVAYFSSQGPLAGDGRIKPDVVAPGYHTFSAQANPAEPGSCR-----------------VAAKAGTSMATPVVAAAAAMIRQYFLQGWYPSGAPNPSD-IHNPSGALVKAVLVNAAVPMTAYRGL-AATVPLGTGPDGYQGWGRVLLDAALNVAGSVDMHAQDMVYMNVT-GMVKTYTFAVDAAYAGDFRATM-VFVEPAGAVGAASPVLNDLDLLVTAFDGYTHSVFHPNGGPGADGVNTVERVTVPDAQAYASFAVTVTASRVATPTQPPFFALVVTG 1647
            C+LSL A +     VC V+ +P +  +N +A WI QSGV+ + P W + L    +II V+DSG+D   C F +  P +    +   N      +RKV++Y  Y D GD   GHGTHV G++ G +            DG+A  AK++ FD G      Y P      + +  Y+AG++  S SWGA   +Y +     D +AY   D +  VAAGN G          G A+   G+V +PA AKN+I+VGASES G     G  G+  +AYFSS+GP + DGR KPD+V+PG+   SA++ P++ G C                  V+ K+GTSMATPV +  AA++RQYF +G+YP+G       I NPS +L+KAV++N A  +    G    ++      D +QG+GR+ L  +L + G+ D   +    ++++ G   +Y++ +    + D  + M V+ +PAG+ G A  ++NDLD L    DG T   ++PNG   AD +N  ER+ +P      + +VTVT + +AT +Q   +ALVVTG
Sbjct:  314 CVLSLVAAIAAQPEVCSVESVPMMKPMNDIAQWITQSGVQNSRPFWDSDLTGAGQIIQVSDSGLDTNHCSFWDSSPGEKRDGTHQPN------RRKVIKYEDYKDDGDYSNGHGTHVVGSIIGHKAKDGVNESSGFIDGMAKDAKVSFFDIGLGTACCYVPGQTT--LFSGGYSAGSKFHSASWGAQTSTYNSNSRGFDNFAYNNKDFLPFVAAGNSGS---------GNAL---GTVGAPATAKNIISVGASESDGRDLYSGSDGLNYLAYFSSRGPTS-DGRRKPDIVSPGHAILSAKSVPSQSGECEPDNASQLPNAGGSSSYAVSYKSGTSMATPVASGTAALVRQYFTEGYYPNGIKGAGTAIANPSASLIKAVMLNGAQSLIGKSGSQGGSISSVAYYDEHQGFGRIQLSTSLPLDGTNDFALEANEKVSISQGDEDSYSYQIGECSSMDELSIMLVWTDPAGSSGCAKCLINDLD-LTAELDGTT---YYPNGRNSADTLNNAERIRLPVTPGD-TVSVTVTGTNLATSSQE--YALVVTG 833