mRNA_18677 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|138406
Unique NamemRNA_18677
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A835YXY9_9STRA (Ubiquitin-conjugating enzyme/RWD-like protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YXY9_9STRA)

HSP 1 Score: 258 bits (659), Expect = 2.050e-87
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 1
Query:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLRR 363
            DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLRR
Sbjct:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLRR 121          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: I0YLH9_COCSC (UBC-like protein (Fragment) n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0YLH9_COCSC)

HSP 1 Score: 166 bits (421), Expect = 1.990e-50
Identity = 79/121 (65.29%), Postives = 87/121 (71.90%), Query Frame = 1
Query:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLRR 363
            +G F FDI LP  YP   PEV FLTTG G VRFNPNLYN GKVCLSLLGTW GP W+P  STLLQV VSIQS+ILV DP+FNEPGYE    TP G   S SYN  IR  TL  A+L+ L++
Sbjct:   14 NGCFLFDILLPPEYPNKPPEVQFLTTGAGRVRFNPNLYNCGKVCLSLLGTWSGPSWDPGTSTLLQVLVSIQSMILVEDPYFNEPGYESSRNTPSGKQHSNSYNANIRRATLEHAVLDTLKK 134          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A0M0JC84_9EUKA (Baculoviral iap repeat-containing protein 6 n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JC84_9EUKA)

HSP 1 Score: 167 bits (422), Expect = 3.730e-50
Identity = 78/121 (64.46%), Postives = 93/121 (76.86%), Query Frame = 1
Query:    4 GLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGP---GWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPL 357
            G F FDI  P  YP VAP+V+  TTG GSVRFNPNLYN GKVCLSLLGTW+G     W P  STLLQV +SIQ+LILVPDP+FNEPG+E + GTP+GD +S  YN+TIR GT+R+A++  L
Sbjct:   24 GCFVFDILAPAEYPNVAPKVNLQTTGGGSVRFNPNLYNCGKVCLSLLGTWQGDDGESWHPKTSTLLQVLMSIQALILVPDPFFNEPGFERNRGTPEGDRQSRQYNETIREGTVRYAMISQL 144          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A7S4DEX5_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DEX5_HETAK)

HSP 1 Score: 171 bits (434), Expect = 2.790e-49
Identity = 81/119 (68.07%), Postives = 89/119 (74.79%), Query Frame = 1
Query:    4 GLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLR 360
            G FEFD+RLP RYP   P+V  +TTG+G VRFNPNLY  GKVCLSLLGTW GPGW P KSTLLQV VSIQSLILVPDP+FNEPGYE  M T +G  ESE YN  +R  TL  AI   L+
Sbjct:   54 GCFEFDLRLPPRYPDCPPQVKLVTTGSGKVRFNPNLYADGKVCLSLLGTWSGPGWVPGKSTLLQVLVSIQSLILVPDPYFNEPGYESQMNTAQGRRESERYNAQVRKDTLMHAIFSQLK 172          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A7R9W000_9CHLO (Hypothetical protein n=1 Tax=Chlamydomonas euryale TaxID=1486919 RepID=A0A7R9W000_9CHLO)

HSP 1 Score: 166 bits (421), Expect = 8.740e-49
Identity = 77/115 (66.96%), Postives = 89/115 (77.39%), Query Frame = 1
Query:    4 GLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAIL 348
            G F FDI LP  YP   P+VHFLTTG G VRFNPNLYN GKVCLSLLGTW GP W+P  STLLQV VSI ++ILVPDP+FNEP YE  M TP+G A S++YN++IRA T+  A+L
Sbjct:  111 GAFVFDILLPPDYPNHPPQVHFLTTGEGRVRFNPNLYNCGKVCLSLLGTWSGPSWQPGTSTLLQVLVSISAMILVPDPFFNEPSYERMMHTPQGCANSKAYNESIRANTMSVAVL 225          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A7S0N848_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0N848_9CRYP)

HSP 1 Score: 160 bits (405), Expect = 4.890e-48
Identity = 76/121 (62.81%), Postives = 88/121 (72.73%), Query Frame = 1
Query:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPLRR 363
            +GLFEFD+ LP  YP V P+V   TTG G VRFNPNLY  G VCLSLLGTW GPGWEP +S+LLQV VSIQSLI+VPDP+FNEPG E     P G   +++YN  IR  TLR A+L  L+R
Sbjct:   30 NGLFEFDVLLPPDYPNVPPQVRLTTTGGGRVRFNPNLYADGTVCLSLLGTWSGPGWEPGRSSLLQVLVSIQSLIMVPDPYFNEPGLESQAKMPAGRRAADNYNAQIRLQTLRVAVLGQLQR 150          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A7S4NL45_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4NL45_9EUKA)

HSP 1 Score: 156 bits (395), Expect = 6.190e-47
Identity = 73/110 (66.36%), Postives = 83/110 (75.45%), Query Frame = 1
Query:   28 LPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPL 357
            LP  YP   P    +TTGNGS RFNPNLY  GKVCLSLLGTW GP W+PN STLLQV VSIQSLILVPDP+FNEPGYE  MGT +G   SE+YN+TI+ GT+  A+ + L
Sbjct:    2 LPHTYPQKPPLCQLVTTGNGSFRFNPNLYACGKVCLSLLGTWSGPAWDPNVSTLLQVLVSIQSLILVPDPYFNEPGYERSMGTRQGKISSENYNKTIKGGTISHAMCDIL 111          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A7S2SX03_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SX03_9STRA)

HSP 1 Score: 168 bits (426), Expect = 6.470e-47
Identity = 77/119 (64.71%), Postives = 92/119 (77.31%), Query Frame = 1
Query:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPL 357
            +G FEFDI LP  YP   P+V  +TTG GSVRFNPNLYN+GKVCLSLLGTW+GPGW+ NKSTLLQV VSIQSLI+VPDP+FNEPG+++ + TP G   S  YN T+R  T+R A+   L
Sbjct:  414 NGCFEFDIWLPAAYPNEPPKVQLMTTGRGSVRFNPNLYNNGKVCLSLLGTWQGPGWDANKSTLLQVLVSIQSLIMVPDPFFNEPGFQNMLNTPAGRLSSSQYNTTVREATVRHAMTAQL 532          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: A0A6V2FBS3_9STRA (Hypothetical protein n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6V2FBS3_9STRA)

HSP 1 Score: 167 bits (423), Expect = 7.580e-47
Identity = 78/115 (67.83%), Postives = 90/115 (78.26%), Query Frame = 1
Query:    1 DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAI 345
            +G F FDI LP  YP V+P+V FLTTG G +RFNPNLYN GKVCLSLLGTW GPGW   +STLLQV +SIQSLILVPDP++NEP Y+   GTPKG A S+SYN+TIR  T+  AI
Sbjct:  289 NGCFLFDINLPGTYPKVSPKVRFLTTGGGKLRFNPNLYNCGKVCLSLLGTWAGPGWVAGQSTLLQVLISIQSLILVPDPYYNEPAYDTSRGTPKGIAASKSYNKTIRGYTISAAI 403          
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Match: D7G0A1_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G0A1_ECTSI)

HSP 1 Score: 170 bits (430), Expect = 1.220e-46
Identity = 79/118 (66.95%), Postives = 89/118 (75.42%), Query Frame = 1
Query:    4 GLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGTWRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESESYNQTIRAGTLRFAILEPL 357
            G FEFDI LP  YP   P+VHFLTTG+G VRFNPNLY +GKVCLSLLGTW GPGW P  STLLQV +SIQSLI V +P+FNEPG+E  +GTP+G   S SYN  IR GTL   +LE L
Sbjct:  761 GCFEFDILLPPDYPNSPPKVHFLTTGSGRVRFNPNLYQTGKVCLSLLGTWAGPGWNPKTSTLLQVLISIQSLIFVAEPYFNEPGFEGQLGTPQGRVASSSYNTRIRNGTLLLGVLEAL 878          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|138406 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YXY9_9STRA2.050e-87100.00Ubiquitin-conjugating enzyme/RWD-like protein (Fra... [more]
I0YLH9_COCSC1.990e-5065.29UBC-like protein (Fragment) n=1 Tax=Coccomyxa sube... [more]
A0A0M0JC84_9EUKA3.730e-5064.46Baculoviral iap repeat-containing protein 6 n=1 Ta... [more]
A0A7S4DEX5_HETAK2.790e-4968.07Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7R9W000_9CHLO8.740e-4966.96Hypothetical protein n=1 Tax=Chlamydomonas euryale... [more]
A0A7S0N848_9CRYP4.890e-4862.81Hypothetical protein (Fragment) n=1 Tax=Cryptomona... [more]
A0A7S4NL45_9EUKA6.190e-4766.36Hypothetical protein (Fragment) n=1 Tax=Paramoeba ... [more]
A0A7S2SX03_9STRA6.470e-4764.71Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A6V2FBS3_9STRA7.580e-4767.83Hypothetical protein n=2 Tax=Ditylum brightwellii ... [more]
D7G0A1_ECTSI1.220e-4666.95Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_274contigContig_274:73328..75536 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|138406|gw1.274.12.1
BRITEko00000,ko00001,ko01000,ko04121,ko04147
KEGG Pathwayko04120,ko04214,ko04215,ko05012,map04120,map04214,map04215,map05012
KEGG koko:K03178,ko:K10586,ko:K10699
EC2.3.2.23,6.2.1.45
Descriptionubiquitin-protein transferase activity
COG categoryO
Max annot lvl2759|Eukaryota
EggNOG OGsCOG5078@1|root,KOG0895@2759|Eukaryota
Evalue2e-47
Seed ortholog2880.D7G0A1
Hectar predicted targeting categoryother localisation
TranscriptId138728
TrackFilteredModels1
ProteinId138406
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|138406gene_18677Tribonema minus UTEX_B_3156 geneContig_274 73328..75536 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_18677_1exon_18677_1Tribonema minus UTEX_B_3156 exonContig_274 73328..73448 +
exon_18677_2exon_18677_2Tribonema minus UTEX_B_3156 exonContig_274 74006..74029 +
exon_18677_3exon_18677_3Tribonema minus UTEX_B_3156 exonContig_274 74874..74982 +
exon_18677_4exon_18677_4Tribonema minus UTEX_B_3156 exonContig_274 75428..75536 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_18677CDS_18677Tribonema minus UTEX_B_3156 CDSContig_274 73328..73448 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|138406Trimin1|138406|gw1.274.12.1Tribonema minus UTEX_B_3156 polypeptideContig_274 73328..75536 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_18677 ID=mRNA_18677|Name=jgi.p|Trimin1|138406|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=363bp|location=Sequence derived from alignment at Contig_274:73328..75536+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
GACGGCCTGTTCGAATTCGATATTCGCTTGCCCCAGAGGTACCCGGTGGT
CGCTCCGGAGGTCCACTTCCTCACCACCGGCAACGGCTCCGTCCGGTTCA
ACCCCAACTTATACAATTCTGGCAAGGTCTGCCTGAGCCTGCTGGGCACC
TGGAGGGGGCCAGGCTGGGAGCCCAACAAGTCCACCCTGCTGCAGGTCTT
TGTCTCCATCCAGTCCCTCATTCTGGTCCCCGACCCGTGGTTCAACGAGC
CGGGGTACGAGCACGACATGGGAACGCCAAAGGGCGATGCGGAGTCTGAG
AGCTACAATCAGACAATCCGCGCCGGCACGCTTCGGTTTGCCATCCTGGA
ACCGTTGCGCCGC
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protein sequence of jgi.p|Trimin1|138406

>Trimin1|138406|gw1.274.12.1 ID=Trimin1|138406|gw1.274.12.1|Name=jgi.p|Trimin1|138406|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=121bp
DGLFEFDIRLPQRYPVVAPEVHFLTTGNGSVRFNPNLYNSGKVCLSLLGT
WRGPGWEPNKSTLLQVFVSIQSLILVPDPWFNEPGYEHDMGTPKGDAESE
SYNQTIRAGTLRFAILEPLRR
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mRNA from alignment at Contig_274:73328..75536+

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_18677 ID=mRNA_18677|Name=jgi.p|Trimin1|138406|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=2209bp|location=Sequence derived from alignment at Contig_274:73328..75536+ (Tribonema minus UTEX_B_3156 )
GACGGCCTGTTCGAATTCGATATTCGCTTGCCCCAGAGGTACCCGGTGGT CGCTCCGGAGGTCCACTTCCTCACCACCGGCAACGGCTCCGTCCGGTTCA ACCCCAACTTATACAATTCTGGTAATTTTCGTGCTATTTATTGCTATAAA TAGTCCCAGTGACATGCTGGAAGTGTGGTATATCATCTGCGGCATGTGCG GCTTGTCACAGATGACCTCGGACTCAAGTTTCAGTCACCTGTGACAGTAC AGCATGCATGCTTTGTAATATGCGCTCTATTCTGCTATTACAAGGTCCAG TAGTATGCTGGAAGTGTGGTAAGTGTGGTACGTCATGCTTTGGTGTGTGC GGCTCTCAGTTGACCTGGGGCTCCTGTTTGAAGCACTCTTGACTTGACAC GTGAGTGCCACTGTAGAGTGCCTGCCCTCGCCATCTTGTTCTTGATTAGA AGTACGTTGCTCATGCCGATGAGGTCGTGTGCACGTGAGGGGTCCTGTAG CTATAGCACTATTGTGAGTGTGGATCAGTTGCATCTAATTGAATGTGACA TCGATTTCTGCATGCAGCTTGTCAGACTGCATGCTGTACCATCATGTTGA TCCAACATGGGTGCAGTCATTTTGCATAATGAATGCTCATACACTTTACA CCCATTGCACTTGCCTGCTTCTTCTCAGGCAAGGTCTGCCTGAGCCTGCT GGGTAAGTCATCACTTGCTCTGTTGACGTTTCGCTGCAGCATGAAAAGTC TTAGATCTAATCACCTGCAGTTTGCTGCCGCAGCCGTTGCGACTCGGATT TTGCGTGCTCAGCCTCCATCTGCTGACCGCCAACACCCTCAGGCTGTGCT TGTACTGCACAGTACCAACGAAGTGTCCGTATCGTGTGATAGGCATCTCA GGGTCTGAATAAGGCATGTCACTGAGTGGTGTGAATATCTGCCATGTTGT GCAATTGACACGTAGGTTTAGAAACAGGTGGTTGTCAAGACAAAGCTGTC GCCGTAGACGTGTGCAGCATCGCCCATGGCAAGTGAAGGACACAAATGGC TTGCAGCTGCCATGCTGCCTTGTCCAATTCTACATCTACCAGCATCACCA CAGAGTACTCATGCATGTGCATGCGTGCATGCATCATGCTGTACGCAGCA AACACGCACCTGAATTGAATGCTGCTGTAGGTCTTTTCAGGTTTTAGGGT AGGTCAGGGTTAGGGCTAGCTCTCAAGCCGCGCCTCATCCCCAACGGTTT GACACCAGCTGTGCCCCTCCCCGCAGCATCGCGGCTCGCTGCGTGTTTGT GCACACCAGTTCTTGCGCCATGAGCATGCTCAGCACGGTGCGAGTCGTTC ACAGCGGTCTCATGTGTCGCCATGCATTGACTTGCTGTGCTTTTGGCGCG CTCCCATTGAACTAAGGCGTGACAGTCCGCCCATGCGAACGGGCATATGC TGCTTGGAGATTTATACCATAAGGACGCTTACCCCTCCCCCCCCCCCCTC ACAAGTATGATGATGTGCTCTCTTTCCGGTGTGAATGCTGCTGCAGGCAC CTGGAGGGGGCCAGGCTGGGAGCCCAACAAGTCCACCCTGCTGCAGGTCT TTGTCTCCATCCAGTCCCTCATTCTGGTCCCCGACCCGTGGTTCAACGAG CCGGGGTAAGTCGAGGTTGAGCAGCTCAGCAACGGCAGCAGCGCTTGGGG TGCACTGGGTCCGTCGCAAATACGCCGTTAGCTTGCACAAACCTGATTGC GTGCTGTTTGGTGTGATACCTGCTGCAGGCGTTTTGTGAGCAAGCAGCGT GCCATTGTGTCGATCACCAGACTGTTCTGTGCTACAGGGCACTGAGCTCA CACATGGCATGTGATTGTCTTGCAGCGATGGCTGTGCTGACGTACGAGAG CAGGGCGCAGGGCGAATGACCGCCGATGCCTGCCGACGCCTTGTATTGCT CGGCGCGTCCATGATGAAAACATGCAGCATTGTTGAAGTTGTGCGCCGCC ACGGACGACTCTCTCTCTCTCTGCATGCAGAGAGCTAGAGTAACAACACC TTGCATATATCGGCTCTGAACACTGAAGCCCAACCTACTGCTCCATGCAG GTACGAGCACGACATGGGAACGCCAAAGGGCGATGCGGAGTCTGAGAGCT ACAATCAGACAATCCGCGCCGGCACGCTTCGGTTTGCCATCCTGGAACCG TTGCGCCGC
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Coding sequence (CDS) from alignment at Contig_274:73328..75536+

>mRNA_18677 ID=mRNA_18677|Name=jgi.p|Trimin1|138406|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=363bp|location=Sequence derived from alignment at Contig_274:73328..75536+ (Tribonema minus UTEX_B_3156 )
GACGGCCTGTTCGAATTCGATATTCGCTTGCCCCAGAGGTACCCGGTGGT
CGCTCCGGAGGTCCACTTCCTCACCACCGGCAACGGCTCCGTCCGGTTCA
ACCCCAACTTATACAATTCTGGCAAGGTCTGCCTGAGCCTGCTGGGCACC
TGGAGGGGGCCAGGCTGGGAGCCCAACAAGTCCACCCTGCTGCAGGTCTT
TGTCTCCATCCAGTCCCTCATTCTGGTCCCCGACCCGTGGTTCAACGAGC
CGGGGTACGAGCACGACATGGGAACGCCAAAGGGCGATGCGGAGTCTGAG
AGCTACAATCAGACAATCCGCGCCGGCACGCTTCGGTTTGCCATCCTGGA
ACCGTTGCGCCGC
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