mRNA_18619 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|181321
Unique NamemRNA_18619
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A835Z283_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z283_9STRA)

HSP 1 Score: 394 bits (1012), Expect = 3.730e-137
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 1
Query:    1 MPFWDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRIGDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQTFVSDVLLFRVVD 735
            MPFWDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRIGDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQTFVSDVLLFRVVD
Sbjct:    1 MPFWDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRIGDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQTFVSDVLLFRVVD 245          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A835Z7T3_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z7T3_9STRA)

HSP 1 Score: 130 bits (327), Expect = 2.850e-31
Identity = 115/200 (57.50%), Postives = 130/200 (65.00%), Query Frame = 1
Query:   16 GAITHILAPLPRGLETLELSAHPDTR--VHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXT---VFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRI 600
            G +T  LAPLP  LE L +S   D    VHPILGQLPQCL VL+L+ +  FNSALG LPPSLTSL LG     XXXXXXXXXXXXXX     V  E  +S +D PLGRLP A++VLDL+   NF            +L LG   XXXXXXXXXXXXXXX      QE+QP LP TLR+FRMG  FN PV LPA+LE + I
Sbjct:  275 GLMTPALAPLPHALEVLTVSTGGDAADSVHPILGQLPQCLTVLDLRSMNTFNSALGLLPPSLTSLRLGSGXXXXXXXXXXXXXXXXXARNPYVIDEDEYSLFDQPLGRLPPAMQVLDLDSNLNFNHTLGCLPVTLRELRLGEAXXXXXXXXXXXXXXXXLGDAFRQEVQPELPSTLRMFRMGARFNVPVALPASLEKVYI 474          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A836C7L7_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C7L7_9STRA)

HSP 1 Score: 113 bits (283), Expect = 1.160e-25
Identity = 76/116 (65.52%), Postives = 81/116 (69.83%), Query Frame = 1
Query:   10 WDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLE 357
            W    TH LAPLP  LETLELS+     VHP+LGQLPQ LRVL+LQ V+ FN ALGPLP SLTSLSLG RFT XXXXXXXXXXXX              D PLGRLPC LRVLDL+
Sbjct:  146 WGSVYTHDLAPLPCALETLELSSRGGAEVHPVLGQLPQRLRVLHLQQVSDFNCALGPLPASLTSLSLGQRFTHXXXXXXXXXXXXRMAD-------GPCDRPLGRLPCRLRVLDLQ 254          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A836CNN2_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNN2_9STRA)

HSP 1 Score: 104 bits (260), Expect = 3.220e-22
Identity = 124/227 (54.63%), Postives = 143/227 (63.00%), Query Frame = 1
Query:   28 HILAPLPRGLETLELSAHPDTRVHPIL-GQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXT-VFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGEC-FNAPVMLPAALEDLRIGDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQT 699
            H LAPL   LETL  +A   +   PIL G+LP CLR L+L+    F SALGPLPP+L+SL L P F + XXXXXXXXX       +F     + +D PLG LP +LR+LDLE     XXXXXXXXXXX KL LG +Y XXXXXXXXXXXXXXXXXXXX                    F APV LPAALE+L IGDSYT  L LRPSL+ + VG  Y   +P LP+T
Sbjct:  247 HPLAPLLHALETLVATAPCTSGQLPILAGELPPCLRTLDLEGCCKFKSALGPLPPNLSSLRLPPWFERDXXXXXXXXXALHMRREIFGAS--AVFDRPLGALPPSLRILDLEGAALXXXXXXXXXXXXRKLRLGDSYSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRFRAPVALPAALEELVIGDSYTLRLTLRPSLKTLLVGAEYRHAIPRLPKT 471          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A836CIY6_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIY6_9STRA)

HSP 1 Score: 99.8 bits (247), Expect = 3.470e-21
Identity = 104/197 (52.79%), Postives = 114/197 (57.87%), Query Frame = 1
Query:   16 GAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHH-------FSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAAL 585
            G +T  LAPLP  LETL +S      VHPILG+LP  L  LNLQ                  L LG  FTQ XXXXXXXXXXX      P  H        S +D PLGRLP A++VL+L+   NF  XXXXXX    +L L   Y XXXXXXXXXXXXX       QELQ  LP TLRVFRMGE FNAPV+LP AL
Sbjct:   73 GLLTPTLAPLPHTLETLTVSTSGAETVHPILGRLPPRLSELNLQGXXXXXXXXXXXXXXXXXLRLGRSFTQAXXXXXXXXXXXH--VAHPYRHPSGDNFARSRFDRPLGRLPAAMQVLNLQYSVNFNHXXXXXXATLRELRLSRAYDXXXXXXXXXXXXXVLGNEFQQELQLELPSTLRVFRMGERFNAPVVLPDAL 267          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A836CJW6_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CJW6_9STRA)

HSP 1 Score: 82.4 bits (202), Expect = 2.530e-14
Identity = 92/201 (45.77%), Postives = 105/201 (52.24%), Query Frame = 1
Query:   34 LAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRIGDSYTHLLDLRP 636
            L  LP  L+T  LS+  DT  H +LG LP+ LR L+L H   FN+ALG LP SLTSLSLG +   XXXXXXXXXXXX   +   E     +D PLG LP ALRVLDL+ C  FXXXXXXXXXXXX+L LG  Y                       L                  AP  LP  L  L IG+ +TH L L P
Sbjct:  305 LTDLPPSLQTPTLSSCHDTP-HIMLGALPRSLRTLDLSHYDNFNNALGRLPASLTSLSLGAKXXXXXXXXXXXXXXXRIRSR-SESGDCAFDRPLGALPPALRVLDLQQCSVFXXXXXXXXXXXXELRLGAAYNWPLAAAPQRAAPPPAAPRQEAALDAVAXXXXXXXXXXXXXAAPAQLPETLTLLHIGEDFTHRLGLLP 503          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A836CCA8_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CCA8_9STRA)

HSP 1 Score: 74.3 bits (181), Expect = 9.230e-12
Identity = 62/133 (46.62%), Postives = 76/133 (57.14%), Query Frame = 1
Query:   34 LAPLPRGLETLELSAHPDTRV---HPILGQLPQCLRVLNLQHVAAFNSALGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLG 423
            LAPLP  L+TLE++   D  V    PIL +LP  L VL++     FN ALG LPP+LT+L LG R+T XXXXXXXXXX     T       S ++ PL  LP  LR+LDL +CD F       X    +L LG
Sbjct:  185 LAPLPSTLKTLEIT-EDDIHVVELQPILARLPPHLEVLDVSQCTNFNIALGQLPPTLTALHLGSRYTHXXXXXXXXXXELQFHTSLGYFPTSLFNCPLEPLPTPLRMLDLSLCDLFGFPLGPLXPALQELRLG 316          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A835ZDX0_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZDX0_9STRA)

HSP 1 Score: 66.2 bits (160), Expect = 9.080e-9
Identity = 74/211 (35.07%), Postives = 90/211 (42.65%), Query Frame = 1
Query:   10 WDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQHVAAFNSA--LGPLPPSLTSLSLGPRFTQXXXXXXXXXXXXXXXTVFPEHHFSHYDHPLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAP----VMLPAALEDLRIGDSYTHLL 624
            W       L  LP  LETL LS   +  +   +G LP  L+ L+    A +NS   LGPLP SLT LSLG  F                 +V        ++ PLG LP +LRVLDL  C+ FXXXXXXXXXXXX+L LG  +                       L+  LP         E    P    V LP  L  L +G S+ H L
Sbjct:  172 WRQRTAVALTVLPESLETLTLSCDAEL-LDLTIGSLPHGLKTLDATRFATYNSTVTLGPLPESLTRLSLGKSFEHALGALPAGLIELRIRSVSGLGGGCFFNQPLGALPPSLRVLDLRACNQFXXXXXXXXXXXXELRLGDAFRHPLLLQHASLPAVQGQQAAAPALEAELP--------AEPVQPPPDALVRLPETLTVLHVGRSFDHPL 373          
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Match: A0A835YKX3_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKX3_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 1.770e-7
Identity = 59/141 (41.84%), Postives = 69/141 (48.94%), Query Frame = 1
Query:  313 PLGRLPCALRVLDLEICDNFXXXXXXXXXXXXKLLLGWTYXXXXXXXXXXXXXXXXXXXXXQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRIGDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQTFVSDVLLFRVVD 735
            PLG+      V+ + +C++F            +L L   Y XXXXXXXXXXXXXXX     QELQPHLPD LR FRMGE F+AP+ LPA                               RPLPPLP TF+ D   F V D
Sbjct:  314 PLGQ------VVGIIVCEDFNHPLGCLPVTLRELRLSCAYDXXXXXXXXXXXXXXXGNAFQQELQPHLPDKLRTFRMGERFDAPIALPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRPLPPLPHTFIFD-RWFAVAD 447          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|181321 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
A0A835Z283_9STRA3.730e-137100.00Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835Z7T3_9STRA2.850e-3157.50Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836C7L7_9STRA1.160e-2565.52Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CNN2_9STRA3.220e-2254.63Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CIY6_9STRA3.470e-2152.79Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CJW6_9STRA2.530e-1445.77Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CCA8_9STRA9.230e-1246.62Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835ZDX0_9STRA9.080e-935.07Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835YKX3_9STRA1.770e-741.84Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_251contigContig_251:96728..97465 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|181321|e_gw1.251.10.1
Hectar predicted targeting categoryother localisation
TranscriptId181643
TrackFilteredModels1
ProteinId181321
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|181321gene_18619Tribonema minus UTEX_B_3156 geneContig_251 96728..97465 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_18619_1exon_18619_1Tribonema minus UTEX_B_3156 exonContig_251 96728..97465 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_18619CDS_18619Tribonema minus UTEX_B_3156 CDSContig_251 96728..97465 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|181321Trimin1|181321|e_gw1.251.10.1Tribonema minus UTEX_B_3156 polypeptideContig_251 96728..97465 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_18619 ID=mRNA_18619|Name=jgi.p|Trimin1|181321|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=738bp|location=Sequence derived from alignment at Contig_251:96728..97465- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGCCCTTCTGGGACGGAGCTATCACGCACATCCTGGCGCCTCTGCCGCG
TGGCCTGGAGACTCTCGAGCTGTCGGCGCACCCCGACACCAGAGTTCACC
CCATCCTGGGCCAGCTGCCGCAGTGCCTCAGAGTCCTCAACCTGCAGCAT
GTCGCCGCCTTCAACAGCGCGCTAGGCCCCCTGCCTCCGAGCCTCACATC
CCTCAGCCTCGGCCCAAGGTTCACACAGCCGCTGGGGGAGCTGCCGCGAG
GCCTCACAGCGCTGACACTCGGCACAGTGTTCCCAGAGCACCACTTCAGT
CACTACGATCACCCGCTGGGGCGCCTGCCCTGTGCGCTGCGCGTGCTCGA
TCTGGAGATTTGCGACAACTTCAACCACCCCCTCGGCTGTCTGCCGGCGA
CGCTCCGCAAGCTACTGCTCGGCTGGACCTACAACCACCCCCTGCAGACG
CTCCCCCCCGCCCTCGAAGTGCTCGTGCTCGGACATGACTTCCAGCAGGA
GCTGCAGCCACATCTGCCAGACACGCTGCGCGTTTTTCGCATGGGCGAGT
GCTTCAACGCGCCGGTCATGCTGCCGGCGGCCCTCGAGGACCTGCGCATC
GGCGACAGTTACACGCACCTGCTGGATCTCAGGCCGTCCTTGAGGCACGT
GTACGTTGGGCTCAGCTACCCGCGCCCGCTGCCGCCTCTGCCGCAGACAT
TTGTATCTGATGTGTTGCTTTTTCGCGTAGTAGATTAG
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protein sequence of jgi.p|Trimin1|181321

>Trimin1|181321|e_gw1.251.10.1 ID=Trimin1|181321|e_gw1.251.10.1|Name=jgi.p|Trimin1|181321|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=246bp
MPFWDGAITHILAPLPRGLETLELSAHPDTRVHPILGQLPQCLRVLNLQH
VAAFNSALGPLPPSLTSLSLGPRFTQPLGELPRGLTALTLGTVFPEHHFS
HYDHPLGRLPCALRVLDLEICDNFNHPLGCLPATLRKLLLGWTYNHPLQT
LPPALEVLVLGHDFQQELQPHLPDTLRVFRMGECFNAPVMLPAALEDLRI
GDSYTHLLDLRPSLRHVYVGLSYPRPLPPLPQTFVSDVLLFRVVD*
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mRNA from alignment at Contig_251:96728..97465-

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_18619 ID=mRNA_18619|Name=jgi.p|Trimin1|181321|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=738bp|location=Sequence derived from alignment at Contig_251:96728..97465- (Tribonema minus UTEX_B_3156 )
ATGCCCTTCTGGGACGGAGCTATCACGCACATCCTGGCGCCTCTGCCGCG TGGCCTGGAGACTCTCGAGCTGTCGGCGCACCCCGACACCAGAGTTCACC CCATCCTGGGCCAGCTGCCGCAGTGCCTCAGAGTCCTCAACCTGCAGCAT GTCGCCGCCTTCAACAGCGCGCTAGGCCCCCTGCCTCCGAGCCTCACATC CCTCAGCCTCGGCCCAAGGTTCACACAGCCGCTGGGGGAGCTGCCGCGAG GCCTCACAGCGCTGACACTCGGCACAGTGTTCCCAGAGCACCACTTCAGT CACTACGATCACCCGCTGGGGCGCCTGCCCTGTGCGCTGCGCGTGCTCGA TCTGGAGATTTGCGACAACTTCAACCACCCCCTCGGCTGTCTGCCGGCGA CGCTCCGCAAGCTACTGCTCGGCTGGACCTACAACCACCCCCTGCAGACG CTCCCCCCCGCCCTCGAAGTGCTCGTGCTCGGACATGACTTCCAGCAGGA GCTGCAGCCACATCTGCCAGACACGCTGCGCGTTTTTCGCATGGGCGAGT GCTTCAACGCGCCGGTCATGCTGCCGGCGGCCCTCGAGGACCTGCGCATC GGCGACAGTTACACGCACCTGCTGGATCTCAGGCCGTCCTTGAGGCACGT GTACGTTGGGCTCAGCTACCCGCGCCCGCTGCCGCCTCTGCCGCAGACAT TTGTATCTGATGTGTTGCTTTTTCGCGTAGTAGATTAG
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Coding sequence (CDS) from alignment at Contig_251:96728..97465-

>mRNA_18619 ID=mRNA_18619|Name=jgi.p|Trimin1|181321|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=738bp|location=Sequence derived from alignment at Contig_251:96728..97465- (Tribonema minus UTEX_B_3156 )
ATGCCCTTCTGGGACGGAGCTATCACGCACATCCTGGCGCCTCTGCCGCG
TGGCCTGGAGACTCTCGAGCTGTCGGCGCACCCCGACACCAGAGTTCACC
CCATCCTGGGCCAGCTGCCGCAGTGCCTCAGAGTCCTCAACCTGCAGCAT
GTCGCCGCCTTCAACAGCGCGCTAGGCCCCCTGCCTCCGAGCCTCACATC
CCTCAGCCTCGGCCCAAGGTTCACACAGCCGCTGGGGGAGCTGCCGCGAG
GCCTCACAGCGCTGACACTCGGCACAGTGTTCCCAGAGCACCACTTCAGT
CACTACGATCACCCGCTGGGGCGCCTGCCCTGTGCGCTGCGCGTGCTCGA
TCTGGAGATTTGCGACAACTTCAACCACCCCCTCGGCTGTCTGCCGGCGA
CGCTCCGCAAGCTACTGCTCGGCTGGACCTACAACCACCCCCTGCAGACG
CTCCCCCCCGCCCTCGAAGTGCTCGTGCTCGGACATGACTTCCAGCAGGA
GCTGCAGCCACATCTGCCAGACACGCTGCGCGTTTTTCGCATGGGCGAGT
GCTTCAACGCGCCGGTCATGCTGCCGGCGGCCCTCGAGGACCTGCGCATC
GGCGACAGTTACACGCACCTGCTGGATCTCAGGCCGTCCTTGAGGCACGT
GTACGTTGGGCTCAGCTACCCGCGCCCGCTGCCGCCTCTGCCGCAGACAT
TTGTATCTGATGTGTTGCTTTTTCGCGTAGTAGATTAG
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