mRNA_16468 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|152099
Unique NamemRNA_16468
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A836CK27_9STRA (RNA recognition motif 2-domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CK27_9STRA)

HSP 1 Score: 256 bits (654), Expect = 1.600e-86
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF
Sbjct:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 125
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A836C7Y7_9STRA (RNA recognition motif 2-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C7Y7_9STRA)

HSP 1 Score: 231 bits (588), Expect = 1.090e-75
Identity = 112/125 (89.60%), Postives = 117/125 (93.60%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LD  KIAKG D+RTTIMIRNIPNKYTQAMLLQEI+ NF   +DFFYLPIDFKNRCNVGYAFINFIDFRRI++FTREFHGQRWRNFNSEKVCAISYARIQGKASMI RFQNSSLMEKDGEYRPLLF
Sbjct:   11 LDIGKIAKGLDKRTTIMIRNIPNKYTQAMLLQEINVNFLEAFDFFYLPIDFKNRCNVGYAFINFIDFRRIIAFTREFHGQRWRNFNSEKVCAISYARIQGKASMICRFQNSSLMEKDGEYRPLLF 135
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: D7FMB4_ECTSI (RRM_2 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FMB4_ECTSI)

HSP 1 Score: 225 bits (574), Expect = 3.600e-67
Identity = 105/125 (84.00%), Postives = 118/125 (94.40%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LD KK++KG D+RTTIM+RNIPNKYTQ MLLQEID+++RG YDFFYLPIDFKN+CNVGYAFINF+D+RRIV F REF+ QRW+NFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPL+F
Sbjct:  621 LDLKKVSKGMDKRTTIMVRNIPNKYTQMMLLQEIDSSYRGAYDFFYLPIDFKNKCNVGYAFINFMDYRRIVPFFREFNAQRWKNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLIF 745
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A7S2V2F9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V2F9_9STRA)

HSP 1 Score: 194 bits (492), Expect = 3.830e-61
Identity = 91/125 (72.80%), Postives = 107/125 (85.60%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LD K++    D+RTT+M+RNIPNKYTQ MLLQEI+ N +  YDFFYLPIDFKN+CNVGYAFINFI    IV F  EF+G++W+NFNSEKVC ISYARIQGK+SMISRFQNSSLM+KDGE++PLLF
Sbjct:   23 LDVKRVLARSDQRTTVMVRNIPNKYTQQMLLQEINVNHKETYDFFYLPIDFKNKCNVGYAFINFISPLSIVEFYEEFNGRKWKNFNSEKVCCISYARIQGKSSMISRFQNSSLMDKDGEFQPLLF 147
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A7S2BIN8_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BIN8_9STRA)

HSP 1 Score: 200 bits (508), Expect = 1.590e-60
Identity = 93/120 (77.50%), Postives = 108/120 (90.00%), Query Frame = 1 
Query:   16 IAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            I +G DRRTT+MIRNIPNKYTQ  +L+EI+ +F+G YDFFYLPIDFKN+CNVGYAFINFI+FR IV F  ++HG+RW NFNSEKVCAI+YARIQGKASMISRFQNSSLM+K GE+RPLLF
Sbjct:  265 IMQGVDRRTTVMIRNIPNKYTQMAVLEEINQHFKGTYDFFYLPIDFKNKCNVGYAFINFIEFRAIVPFVCKYHGRRWNNFNSEKVCAITYARIQGKASMISRFQNSSLMDKSGEFRPLLF 384
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A7S2SS48_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SS48_9STRA)

HSP 1 Score: 207 bits (526), Expect = 6.130e-60
Identity = 97/125 (77.60%), Postives = 113/125 (90.40%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            L+  ++ +G DRRTT+M+RNIPNKYTQA +L+EI+ NF+G YDFFYLPIDFKN+CNVGYAF+NFIDFR IVSF  +F+G+RW NFNSEKVCAI+YARIQGK SMISRFQNSSLMEKDGEYRPLLF
Sbjct:  730 LNIDRVMQGSDRRTTLMVRNIPNKYTQAAVLEEINENFQGLYDFFYLPIDFKNKCNVGYAFMNFIDFRSIVSFFCKFNGRRWNNFNSEKVCAITYARIQGKTSMISRFQNSSLMEKDGEYRPLLF 854
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A4D9D2V8_9STRA (RRM domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D2V8_9STRA)

HSP 1 Score: 204 bits (520), Expect = 1.160e-58
Identity = 97/125 (77.60%), Postives = 110/125 (88.00%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LD ++I  G DRRTTIM+RN+PNKY+Q +LL EI+A  +G YDFFYLPIDFKN+CNVGYAFINFI+ + IV F +EF+GQRWRNFNSEKVCAISYARIQGK SMISRF NSSLMEKDGEYRPLLF
Sbjct: 1109 LDVERILMGMDRRTTIMVRNVPNKYSQQLLLNEINATHKGKYDFFYLPIDFKNKCNVGYAFINFIEPKAIVPFFKEFNGQRWRNFNSEKVCAISYARIQGKQSMISRFSNSSLMEKDGEYRPLLF 1233
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A482RU13_9ARCH (RRM domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RU13_9ARCH)

HSP 1 Score: 186 bits (473), Expect = 3.070e-58
Identity = 83/125 (66.40%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            LD +++  G + RTT+M+RNIPNKY+Q MLL+E++A  +G YDFFYLPIDFKNRCNVGYAFINF+    IV F R F GQRWR+FNSEK+C+++YARIQGKA+++SRFQNSSL++KD  Y+PLLF
Sbjct:   32 LDLQRLQDGSETRTTLMVRNIPNKYSQQMLLEEVNAQHQGQYDFFYLPIDFKNRCNVGYAFINFLSPLHIVDFVRRFEGQRWRSFNSEKICSVTYARIQGKAALVSRFQNSSLLDKDDTYKPLLF 156
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A7S3Y2P3_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y2P3_HETAK)

HSP 1 Score: 186 bits (472), Expect = 4.960e-58
Identity = 87/125 (69.60%), Postives = 103/125 (82.40%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            L+ ++I  G D RTT+M+RNIPNKYTQ MLL EI+A F G YDFFYLPIDFKNRCNVGYAF+NF+  R I +F R F GQRW  FNSEKVCAI+YAR+QG+ +MI+RFQNSSL+ K+G YRPLLF
Sbjct:   29 LNMQQILDGADLRTTVMVRNIPNKYTQQMLLDEINAEFEGTYDFFYLPIDFKNRCNVGYAFVNFLSARTIPAFHRRFDGQRWGTFNSEKVCAIAYARLQGRRAMIARFQNSSLLAKEGRYRPLLF 153
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BLAST of jgi.p|Trimin1|152099 vs. uniprot
Match: A0A3R7KLF4_9STRA (RRM_2 domain-containing protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7KLF4_9STRA)

HSP 1 Score: 190 bits (482), Expect = 8.780e-57
Identity = 89/125 (71.20%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPIDFKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQGKASMISRFQNSSLMEKDGEYRPLLF 375
            L  +++A G+D RTT+MIRNIPNKYTQ MLL EI+ N RG YDFFYLPIDFKN+CN+GYAFINFI+   I +F REF GQ+W NFNSEKVCAISYAR+QGK +MI+RFQNSSL+EK   YRPL+F
Sbjct:  233 LSIERVASGEDMRTTLMIRNIPNKYTQQMLLAEINRNHRGNYDFFYLPIDFKNKCNMGYAFINFIEAAHIEAFHREFDGQKWTNFNSEKVCAISYARLQGKQAMIARFQNSSLLEKHESYRPLVF 357
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|152099 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CK27_9STRA1.600e-86100.00RNA recognition motif 2-domain-containing protein ... [more]
A0A836C7Y7_9STRA1.090e-7589.60RNA recognition motif 2-domain-containing protein ... [more]
D7FMB4_ECTSI3.600e-6784.00RRM_2 domain-containing protein n=2 Tax=Ectocarpus... [more]
A0A7S2V2F9_9STRA3.830e-6172.80Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S2BIN8_9STRA1.590e-6077.50Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S2SS48_9STRA6.130e-6077.60Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A4D9D2V8_9STRA1.160e-5877.60RRM domain-containing protein n=2 Tax=Monodopsidac... [more]
A0A482RU13_9ARCH3.070e-5866.40RRM domain-containing protein (Fragment) n=1 Tax=a... [more]
A0A7S3Y2P3_HETAK4.960e-5869.60Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A3R7KLF4_9STRA8.780e-5771.20RRM_2 domain-containing protein n=3 Tax=Phytophtho... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_162contigContig_162:208569..209587 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|152099|gw1.162.20.1
GOsGO:0000003,GO:0000228,GO:0000280,GO:0003674,GO:0003676,GO:0003723,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005737,GO:0006275,GO:0006996,GO:0007049,GO:0007127,GO:0007154,GO:0007165,GO:0007346,GO:0008150,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010556,GO:0010557,GO:0010564,GO:0010571,GO:0010604,GO:0010638,GO:0016043,GO:0019219,GO:0019222,GO:0022402,GO:0022414,GO:0023052,GO:0030174,GO:0031136,GO:0031137,GO:0031139,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032298,GO:0032991,GO:0033043,GO:0033044,GO:0033045,GO:0033262,GO:0033620,GO:0034064,GO:0040020,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043900,GO:0043902,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045740,GO:0045786,GO:0045787,GO:0045836,GO:0045930,GO:0045935,GO:0046999,GO:0048285,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0050789,GO:0050794,GO:0050896,GO:0051052,GO:0051054,GO:0051128,GO:0051130,GO:0051171,GO:0051173,GO:0051321,GO:0051445,GO:0051446,GO:0051716,GO:0051726,GO:0051728,GO:0051783,GO:0051785,GO:0051983,GO:0051984,GO:0060184,GO:0060255,GO:0061982,GO:0065007,GO:0070013,GO:0071840,GO:0080090,GO:0090068,GO:0090329,GO:0097159,GO:0140013,GO:1901363,GO:1901987,GO:1901989,GO:1901993,GO:1901995,GO:1902099,GO:1902101,GO:1902102,GO:1902104,GO:1903046,GO:1904512,GO:1904514,GO:1905132,GO:1905134,GO:1905189,GO:1905191,GO:1905818,GO:1905820,GO:1990904,GO:2000105,GO:2000112,GO:2000241,GO:2000243
DescriptionRNA binding
COG categoryP
Max annot lvl2759|Eukaryota
EggNOG OGsKOG4660@1|root,KOG4660@2759|Eukaryota
Evalue5.91e-68
Seed ortholog2880.D7FMB4
Hectar predicted targeting categoryother localisation
TranscriptId152421
TrackFilteredModels1
ProteinId152099
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|152099gene_16468Tribonema minus UTEX_B_3156 geneContig_162 208569..209587 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|152099Trimin1|152099|gw1.162.20.1Tribonema minus UTEX_B_3156 polypeptideContig_162 208569..209587 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_16468_3exon_16468_3Tribonema minus UTEX_B_3156 exonContig_162 208569..208676 -
exon_16468_2exon_16468_2Tribonema minus UTEX_B_3156 exonContig_162 209049..209234 -
exon_16468_1exon_16468_1Tribonema minus UTEX_B_3156 exonContig_162 209507..209587 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_16468CDS_16468Tribonema minus UTEX_B_3156 CDSContig_162 209507..209587 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_16468 ID=mRNA_16468|Name=jgi.p|Trimin1|152099|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=375bp|location=Sequence derived from alignment at Contig_162:208569..209587- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
CTGGACACCAAGAAGATAGCCAAGGGGCAGGACCGTCGCACAACAATCAT
GATCCGCAATATCCCCAACAAGTACACGCAGGCAATGCTTCTGCAAGAGA
TTGATGCCAACTTCCGCGGCGGGTACGACTTCTTCTACCTGCCGATCGAC
TTCAAGAATCGTTGCAACGTGGGCTACGCGTTCATCAACTTTATCGACTT
CCGCCGCATAGTGTCGTTCACGCGCGAGTTCCACGGGCAGCGCTGGCGCA
ACTTCAATTCTGAGAAGGTGTGCGCCATCAGTTATGCGCGCATCCAGGGC
AAGGCCTCCATGATTTCGAGGTTCCAAAACTCGTCACTCATGGAGAAGGA
TGGCGAGTACCGTCCCCTCCTGTTC
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protein sequence of jgi.p|Trimin1|152099

>Trimin1|152099|gw1.162.20.1 ID=Trimin1|152099|gw1.162.20.1|Name=jgi.p|Trimin1|152099|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=125bp
LDTKKIAKGQDRRTTIMIRNIPNKYTQAMLLQEIDANFRGGYDFFYLPID
FKNRCNVGYAFINFIDFRRIVSFTREFHGQRWRNFNSEKVCAISYARIQG
KASMISRFQNSSLMEKDGEYRPLLF
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mRNA from alignment at Contig_162:208569..209587-

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_16468 ID=mRNA_16468|Name=jgi.p|Trimin1|152099|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1019bp|location=Sequence derived from alignment at Contig_162:208569..209587- (Tribonema minus UTEX_B_3156 )
CTGGACACCAAGAAGATAGCCAAGGGGCAGGACCGTCGCACAACAATCAT
GATCCGCAATATCCCCAACAAGTACACGCAGGTGAGCACATGGCCAGAGT
GAGCATCGCAGGGCCACTGATTGTACCCCACAATAGCTCGTACTCTGCAG
ATGTATGTGTACGATGGCATGTGGTGTGACAATGCACTACATGGGTGCAA
CGCTGGCTTGCTTGGCAATCTTTGTCATGCCATCATTTACCTCGCCTAAC
TTGTGTGCCTACTTTTGCAAAGTATCATATCCCAATACCACCTTGCATAG
CGCTCCTGAAATCCTTTCTGACTGGCACCTCGATGTCCTGCTTGTGCATG
CAGGCAATGCTTCTGCAAGAGATTGATGCCAACTTCCGCGGCGGGTACGA
CTTCTTCTACCTGCCGATCGACTTCAAGAATCGTTGCAACGTGGGCTACG
CGTTCATCAACTTTATCGACTTCCGCCGCATAGTGTCGTTCACGCGCGAG
TTCCACGGGCAGCGCTGGCGCAACTTCAATTCTGAGAAGGTGTGTGTGTG
TGTTGTGCGTGTGTGTGCGTGGTGCGAACGCCTCCTCGCACCCCATGCAG
GTCCCCATGCCTCATCCTTTGCTCTCACTGATGCATGGTGCCCAGGTGCT
CTATCTATACCAATGCCAGCCTGTCCAGTGGCTCAATGGCGTGCATTAAT
TTGGTGACCTGAAGCTGCAACAAGCACCTGCTTGAGCATGGGAGTATGCA
GTTCATGATCATACGTGTTCCGGAGCTGTCCGTGTGCAGCATCTTGTTGT
GCCGTCCACTGCCACACAGCTCTAGCAACAGTGCAGAAGCTATGGTGTAC
TTGCAGTATCCACCTACCTTTCCACTATGCTTCTTCCATCTGCTCCGTCG
CACCCCTCCAGGTGTGCGCCATCAGTTATGCGCGCATCCAGGGCAAGGCC
TCCATGATTTCGAGGTTCCAAAACTCGTCACTCATGGAGAAGGATGGCGA
GTACCGTCCCCTCCTGTTC
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Coding sequence (CDS) from alignment at Contig_162:208569..209587-

>mRNA_16468 ID=mRNA_16468|Name=jgi.p|Trimin1|152099|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=375bp|location=Sequence derived from alignment at Contig_162:208569..209587- (Tribonema minus UTEX_B_3156 )
CTGGACACCAAGAAGATAGCCAAGGGGCAGGACCGTCGCACAACAATCAT
GATCCGCAATATCCCCAACAAGTACACGCAGGCAATGCTTCTGCAAGAGA
TTGATGCCAACTTCCGCGGCGGGTACGACTTCTTCTACCTGCCGATCGAC
TTCAAGAATCGTTGCAACGTGGGCTACGCGTTCATCAACTTTATCGACTT
CCGCCGCATAGTGTCGTTCACGCGCGAGTTCCACGGGCAGCGCTGGCGCA
ACTTCAATTCTGAGAAGGTGTGCGCCATCAGTTATGCGCGCATCCAGGGC
AAGGCCTCCATGATTTCGAGGTTCCAAAACTCGTCACTCATGGAGAAGGA
TGGCGAGTACCGTCCCCTCCTGTTC
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