mRNA_S-firma_F_contig10196.305.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig10196.305.1
Unique NamemRNA_S-firma_F_contig10196.305.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A0D1DYL9_USTMA (Uncharacterized protein n=1 Tax=Ustilago maydis (strain 521 / FGSC 9021) TaxID=237631 RepID=A0A0D1DYL9_USTMA)

HSP 1 Score: 68.9 bits (167), Expect = 9.180e-11
Identity = 43/102 (42.16%), Postives = 61/102 (59.80%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGSIVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL  G  G  +VS S+DK  K+WR+  CV    GH  +V+ V V++    +++ SAD T ++   S++ + +A   GHTD VRGL +LE GE
Sbjct:  120 TLIGHTENVCALDAGPHGQYLVSGSWDKTAKIWRNWECVATLKGHEQSVWAV-VAVDHDRVLTASADKTIRLWSISNSSKPLAIFGGHTDAVRGLTLLEGGE 220
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A2N8UEZ5_9BASI (Related to DOA1-involved in ubiquitin-dependent proteolysis n=2 Tax=Sporisorium reilianum TaxID=72558 RepID=A0A2N8UEZ5_9BASI)

HSP 1 Score: 68.6 bits (166), Expect = 1.250e-10
Identity = 46/103 (44.66%), Postives = 60/103 (58.25%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL  G  G  +VS S+DK  K+WR+  CV    GH  +V+  AV  VD   +++ SAD T ++   SS  + +A   GHTD VRGL +LE GE
Sbjct:  121 TLIGHTENVCALDVGPHGQYLVSGSWDKTAKIWRNWECVATLKGHEQSVW--AVLAVDQDRVLTASADKTIRLWSISSPAKPLAVFGGHTDAVRGLTLLEGGE 221
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: V5EJL2_KALBG (Uncharacterized protein n=1 Tax=Kalmanozyma brasiliensis (strain GHG001) TaxID=1365824 RepID=V5EJL2_KALBG)

HSP 1 Score: 68.6 bits (166), Expect = 1.310e-10
Identity = 48/103 (46.60%), Postives = 60/103 (58.25%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL  G  G  IVS S+DK  KVWR+  CV    GH  +V+  AV  VD   +++ SAD T ++   SS  + +A   GHTD VRGL +LE GE
Sbjct:  704 TLIGHTENVCALDVGPHGQYIVSGSWDKTAKVWRNWECVATFKGHEQSVW--AVLAVDQDRVLTASADKTIRLWSISSPTKPLAVFGGHTDAVRGLTLLEGGE 804
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: S3C523_OPHP1 (Ubiquitin homeostasis protein lub1 n=1 Tax=Ophiostoma piceae (strain UAMH 11346) TaxID=1262450 RepID=S3C523_OPHP1)

HSP 1 Score: 67.8 bits (164), Expect = 2.290e-10
Identity = 40/112 (35.71%), Postives = 61/112 (54.46%), Query Frame = 1 
Query:   94 ATGHHPVIRLWSGEGVLEATLEGHTSAVPALTCGASGDIVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGSIVSGSADNTAKVCSSTGECVA---TLKGHTDWVR 420
            + G+  +IRLW   G     L GH S + AL C  +G++VS S D+ V++WR   CV       L+++ V+V    G   +G++DN A+V S   + VA   TLK  +D V+
Sbjct:  231 SAGNDFMIRLWQFNGTEVGALHGHDSFIYALDCLPTGELVSSSEDRTVRIWRGTACVQTITHPALSIWSVSVCQKTGDFATGASDNVARVFSRNKDRVASADTLKQFSDSVQ 342
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A5C3FAF0_9BASI (Related to DOA1 - involved in ubiquitin-dependent proteolysis n=1 Tax=Pseudozyma flocculosa TaxID=84751 RepID=A0A5C3FAF0_9BASI)

HSP 1 Score: 67.8 bits (164), Expect = 2.310e-10
Identity = 52/140 (37.14%), Postives = 75/140 (53.57%), Query Frame = 1 
Query:   49 EDPVFSIVAVPGGGFATGHHPVIRLWSGEGVLEAT-----LEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGSIVSGSADNTAKVCS--STGECVATLKGHTDWVRGLCVLENG 444
            +DP  SIVA+  G     H   ++  +G+GV  +      + GH   V  L  G  G  IVS S+DK  KVWR+  CV    GH  AV+ V V++ +  I++ SAD T ++ S  +    +AT  GH+D VRGL +L+ G
Sbjct:   75 QDPSGSIVALTAGQDRIVHGFEVQA-TGDGVKASAAPDYAMIGHEDNVCTLDVGPRGSYIVSGSWDKTAKVWREFQCVATLKGHQQAVWAV-VAVDEDRILTASADKTIRLWSIATPDRPLATFTGHSDAVRGLVLLDGG 212
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A077R4W5_9BASI (Related to DOA1-involved in ubiquitin-dependent proteolysis n=1 Tax=Melanopsichium pennsylvanicum 4 TaxID=1398559 RepID=A0A077R4W5_9BASI)

HSP 1 Score: 67.8 bits (164), Expect = 2.320e-10
Identity = 48/103 (46.60%), Postives = 59/103 (57.28%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL     G  IVS S+DK  KVWR+  CV    GH  +V+  AV  VD   +++ SAD T K+   SS  + +A   GHTD VRGL +LE GE
Sbjct:  120 TLIGHTENVCALDVEQHGKYIVSGSWDKTAKVWRNWECVATFKGHEQSVW--AVLAVDHDRVLTASADKTIKLWSISSPSKPIAVFGGHTDAVRGLTLLEGGE 220
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: R9PD22_PSEHS (Uncharacterized protein n=1 Tax=Pseudozyma hubeiensis (strain SY62) TaxID=1305764 RepID=R9PD22_PSEHS)

HSP 1 Score: 67.4 bits (163), Expect = 3.160e-10
Identity = 46/103 (44.66%), Postives = 60/103 (58.25%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL   A G  +VS S+DK  K+WRD  CV    GH  +V+  AV  VD   +++ SAD T ++   S+  + +A   GHTD VRGL +LE GE
Sbjct:  120 TLIGHTENVCALDVDAHGRYLVSGSWDKTAKIWRDWECVATLKGHEQSVW--AVLAVDQDRVLTASADKTIRLWSISNPSKPLAIFGGHTDAVRGLTLLEGGE 220
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A0F7S152_9BASI (Related to DOA1-involved in ubiquitin-dependent proteolysis n=1 Tax=Sporisorium scitamineum TaxID=49012 RepID=A0A0F7S152_9BASI)

HSP 1 Score: 66.6 bits (161), Expect = 5.850e-10
Identity = 45/103 (43.69%), Postives = 59/103 (57.28%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKVCSSTGEC--VATLKGHTDWVRGLCVLENGE 447
            TL GHT  V AL  G  G  +VS S+DK  K+WR+  CV    GH  +V+  AV  VD   +++ SAD T ++ S +     +A   GHTD VRGL +LE GE
Sbjct:  120 TLIGHTENVCALDVGPHGQYLVSGSWDKTAKIWRNWECVATLKGHEQSVW--AVLAVDQDRVLTASADKTIRLWSISNPTKPLAVFGGHTDAVRGLTLLEGGE 220
[Close]
BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: I2FYN0_USTH4 (Related to DOA1-involved in ubiquitin-dependent proteolysis n=2 Tax=Ustilago TaxID=5269 RepID=I2FYN0_USTH4)

HSP 1 Score: 66.2 bits (160), Expect = 7.970e-10
Identity = 46/103 (44.66%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGS-IVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GH   V  L  G  G  IVS S+DK  KVWR+  CV    GH  +V+  AV  VD   +++ SAD T ++   SS  + +A   GHTD VRGL +LE GE
Sbjct:  120 TLVGHQENVCTLDIGPHGQYIVSGSWDKTAKVWRNWECVATLKGHEQSVW--AVLAVDHDRVLTASADKTIRLWSISSPAKPLAVFGGHTDAVRGLTLLEGGE 220
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BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Match: A0A066V922_TILAU (WD40 repeat-like protein (Fragment) n=1 Tax=Tilletiaria anomala (strain ATCC 24038 / CBS 436.72 / UBC 951) TaxID=1037660 RepID=A0A066V922_TILAU)

HSP 1 Score: 65.9 bits (159), Expect = 8.600e-10
Identity = 41/102 (40.20%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  151 TLEGHTSAVPALTCGASGD-IVSCSYDKAVKVWRDHTCVLACVGHTLAVFCVAVSLVDGSIVSGSADNTAKV--CSSTGECVATLKGHTDWVRGLCVLENGE 447
            TL GH   + +L  G  G  IVS S+DK  KVW+D  CV    GH  AV+ V +++ +  I++ SAD   ++   SS  + +AT  GH D VRGL +L+ G+
Sbjct:   65 TLLGHEENICSLDAGPGGSYIVSGSWDKTAKVWKDWKCVATLKGHAHAVWAV-LAVDEDRILTASADKLIRLWSISSPSKPIATFSGHLDAVRGLSLLQGGK 165
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig10196.305.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0D1DYL9_USTMA9.180e-1142.16Uncharacterized protein n=1 Tax=Ustilago maydis (s... [more]
A0A2N8UEZ5_9BASI1.250e-1044.66Related to DOA1-involved in ubiquitin-dependent pr... [more]
V5EJL2_KALBG1.310e-1046.60Uncharacterized protein n=1 Tax=Kalmanozyma brasil... [more]
S3C523_OPHP12.290e-1035.71Ubiquitin homeostasis protein lub1 n=1 Tax=Ophiost... [more]
A0A5C3FAF0_9BASI2.310e-1037.14Related to DOA1 - involved in ubiquitin-dependent ... [more]
A0A077R4W5_9BASI2.320e-1046.60Related to DOA1-involved in ubiquitin-dependent pr... [more]
R9PD22_PSEHS3.160e-1044.66Uncharacterized protein n=1 Tax=Pseudozyma hubeien... [more]
A0A0F7S152_9BASI5.850e-1043.69Related to DOA1-involved in ubiquitin-dependent pr... [more]
I2FYN0_USTH47.970e-1044.66Related to DOA1-involved in ubiquitin-dependent pr... [more]
A0A066V922_TILAU8.600e-1040.20WD40 repeat-like protein (Fragment) n=1 Tax=Tillet... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig10196contigS-firma_F_contig10196:4441..4887 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog55529.EKX49868
Preferred namePLAA
PFAMsPFU,PUL,WD40
Max annot lvl2759|Eukaryota
KEGG rclassRC00002,RC00010,RC00043,RC02750,RC02798,RC03314
KEGG koko:K01955,ko:K14018
KEGG ReactionR00256,R00575,R01395,R10948,R10949
KEGG Pathwayko00240,ko00250,ko01100,ko04141,map00240,map00250,map01100,map04141
KEGG ModuleM00051
GOsGO:0000726,GO:0001558,GO:0002237,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005615,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0006082,GO:0006139,GO:0006259,GO:0006281,GO:0006302,GO:0006303,GO:0006325,GO:0006464,GO:0006508,GO:0006511,GO:0006629,GO:0006631,GO:0006644,GO:0006690,GO:0006692,GO:0006693,GO:0006725,GO:0006793,GO:0006796,GO:0006807,GO:0006914,GO:0006950,GO:0006952,GO:0006954,GO:0006974,GO:0006996,GO:0007154,GO:0007165,GO:0007275,GO:0007399,GO:0008047,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009605,GO:0009607,GO:0009617,GO:0009892,GO:0009987,GO:0010033,GO:0010498,GO:0010517,GO:0010518,GO:0010605,GO:0010720,GO:0010975,GO:0010976,GO:0010992,GO:0016004,GO:0016005,GO:0016043,GO:0016236,GO:0016567,GO:0016569,GO:0016570,GO:0016574,GO:0019222,GO:0019538,GO:0019637,GO:0019725,GO:0019752,GO:0019941,GO:0022008,GO:0022603,GO:0022604,GO:0023052,GO:0030154,GO:0030163,GO:0030234,GO:0030307,GO:0030334,GO:0030335,GO:0031323,GO:0031324,GO:0031344,GO:0031346,GO:0031396,GO:0031397,GO:0031399,GO:0031400,GO:0031982,GO:0032182,GO:0032268,GO:0032269,GO:0032429,GO:0032430,GO:0032446,GO:0032496,GO:0032501,GO:0032502,GO:0032787,GO:0032879,GO:0033554,GO:0033559,GO:0033993,GO:0034517,GO:0034641,GO:0035973,GO:0036211,GO:0040008,GO:0040012,GO:0040017,GO:0042221,GO:0042592,GO:0043085,GO:0043130,GO:0043161,GO:0043162,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043230,GO:0043231,GO:0043412,GO:0043436,GO:0043632,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044255,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044281,GO:0044421,GO:0044424,GO:0044464,GO:0045202,GO:0045595,GO:0045597,GO:0045664,GO:0045666,GO:0045927,GO:0046483,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048638,GO:0048639,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050769,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0050896,GO:0051049,GO:0051050,GO:0051094,GO:0051128,GO:0051130,GO:0051171,GO:0051172,GO:0051239,GO:0051240,GO:0051246,GO:0051248,GO:0051270,GO:0051272,GO:0051276,GO:0051336,GO:0051345,GO:0051603,GO:0051704,GO:0051707,GO:0051716,GO:0051960,GO:0051962,GO:0060191,GO:0060193,GO:0060229,GO:0060255,GO:0060284,GO:0060341,GO:0061912,GO:0061919,GO:0065007,GO:0065008,GO:0065009,GO:0070062,GO:0070647,GO:0070887,GO:0071216,GO:0071219,GO:0071222,GO:0071310,GO:0071396,GO:0071704,GO:0071840,GO:0072671,GO:0080090,GO:0090304,GO:0098693,GO:0098772,GO:0120035,GO:1900044,GO:1900045,GO:1901360,GO:1901564,GO:1901565,GO:1901568,GO:1901575,GO:1901700,GO:1901701,GO:1902914,GO:1902915,GO:1903320,GO:1903321,GO:1903421,GO:1903423,GO:1903561,GO:1903859,GO:1903861,GO:2000026,GO:2000145,GO:2000147,GO:2001222,GO:2001224
Evalue9.03e-15
EggNOG OGsKOG0301@1|root,KOG0301@2759|Eukaryota
EC6.3.5.5
Descriptionphospholipase A2 activator activity
COG categoryA
BRITEko00000,ko00001,ko00002,ko01000
Hectar predicted targeting categoryother localisation
Exons1
Model size447
Cds size447
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815547.5720139-CDS-S-firma_F_contig10196:4440..48871622815547.5720139-CDS-S-firma_F_contig10196:4440..4887Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10196 4441..4887 -
1696949407.1356182-CDS-S-firma_F_contig10196:4440..48871696949407.1356182-CDS-S-firma_F_contig10196:4440..4887Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10196 4441..4887 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig10196.305.1prot_S-firma_F_contig10196.305.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig10196 4441..4887 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig10196.305.1

>prot_S-firma_F_contig10196.305.1 ID=prot_S-firma_F_contig10196.305.1|Name=mRNA_S-firma_F_contig10196.305.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=149bp
MASPACFEEVAFFSLQEDPVFSIVAVPGGGFATGHHPVIRLWSGEGVLEA
TLEGHTSAVPALTCGASGDIVSCSYDKAVKVWRDHTCVLACVGHTLAVFC
VAVSLVDGSIVSGSADNTAKVCSSTGECVATLKGHTDWVRGLCVLENGE
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mRNA from alignment at S-firma_F_contig10196:4441..4887-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig10196.305.1 ID=mRNA_S-firma_F_contig10196.305.1|Name=mRNA_S-firma_F_contig10196.305.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=447bp|location=Sequence derived from alignment at S-firma_F_contig10196:4441..4887- (Sphaerotrichia firma ET2_F female)
ATGGCAAGTCCTGCATGCTTTGAGGAAGTGGCCTTCTTTTCCCTGCAAGA
AGACCCCGTGTTTAGCATTGTGGCGGTGCCTGGGGGTGGTTTTGCAACCG
GACACCACCCTGTCATTCGCTTGTGGAGTGGGGAGGGCGTGCTGGAGGCC
ACGCTGGAGGGCCACACAAGCGCAGTTCCAGCATTGACGTGCGGTGCATC
AGGAGACATTGTGTCCTGCTCCTACGACAAGGCGGTGAAAGTTTGGCGTG
ACCACACCTGCGTCCTCGCCTGTGTCGGCCACACCCTCGCCGTTTTCTGT
GTGGCAGTGTCCCTGGTGGACGGCAGCATCGTCAGCGGCTCCGCGGATAA
CACGGCCAAGGTGTGTTCGTCGACGGGGGAGTGCGTGGCCACCCTGAAGG
GCCACACGGATTGGGTGAGAGGGTTGTGCGTGCTGGAGAACGGCGAG
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Coding sequence (CDS) from alignment at S-firma_F_contig10196:4441..4887-

>mRNA_S-firma_F_contig10196.305.1 ID=mRNA_S-firma_F_contig10196.305.1|Name=mRNA_S-firma_F_contig10196.305.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=894bp|location=Sequence derived from alignment at S-firma_F_contig10196:4441..4887- (Sphaerotrichia firma ET2_F female)
ATGGCAAGTCCTGCATGCTTTGAGGAAGTGGCCTTCTTTTCCCTGCAAGA
AGACCCCGTGTTTAGCATTGTGGCGGTGCCTGGGGGTGGTTTTGCAACCG
GACACCACCCTGTCATTCGCTTGTGGAGTGGGGAGGGCGTGCTGGAGGCC
ACGCTGGAGGGCCACACAAGCGCAGTTCCAGCATTGACGTGCGGTGCATC
AGGAGACATTGTGTCCTGCTCCTACGACAAGGCGGTGAAAGTTTGGCGTG
ACCACACCTGCGTCCTCGCCTGTGTCGGCCACACCCTCGCCGTTTTCTGT
GTGGCAGTGTCCCTGGTGGACGGCAGCATCGTCAGCGGCTCCGCGGATAA
CACGGCCAAGGTGTGTTCGTCGACGGGGGAGTGCGTGGCCACCCTGAAGG
GCCACACGGATTGGGTGAGAGGGTTGTGCGTGCTGGAGAACGGCGAGATG
GCAAGTCCTGCATGCTTTGAGGAAGTGGCCTTCTTTTCCCTGCAAGAAGA
CCCCGTGTTTAGCATTGTGGCGGTGCCTGGGGGTGGTTTTGCAACCGGAC
ACCACCCTGTCATTCGCTTGTGGAGTGGGGAGGGCGTGCTGGAGGCCACG
CTGGAGGGCCACACAAGCGCAGTTCCAGCATTGACGTGCGGTGCATCAGG
AGACATTGTGTCCTGCTCCTACGACAAGGCGGTGAAAGTTTGGCGTGACC
ACACCTGCGTCCTCGCCTGTGTCGGCCACACCCTCGCCGTTTTCTGTGTG
GCAGTGTCCCTGGTGGACGGCAGCATCGTCAGCGGCTCCGCGGATAACAC
GGCCAAGGTGTGTTCGTCGACGGGGGAGTGCGTGGCCACCCTGAAGGGCC
ACACGGATTGGGTGAGAGGGTTGTGCGTGCTGGAGAACGGCGAG
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