mRNA_S-firma_F_contig10371.533.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig10371.533.1
Unique NamemRNA_S-firma_F_contig10371.533.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A835YRJ7_9STRA (Glycine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRJ7_9STRA)

HSP 1 Score: 162 bits (409), Expect = 1.220e-45
Identity = 75/90 (83.33%), Postives = 81/90 (90.00%), Query Frame = 1 
Query:    1 RDVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDE 270
            RDVH    LAHYARACTDITF+FPFG+SELQGVAARG YDLTQHQE+SGK MEYFDE +K KYVPHVIEPSLGVDRLFLA++ SAYHEDE
Sbjct:  293 RDVHEAHKLAHYARACTDITFRFPFGQSELQGVAARGAYDLTQHQEASGKGMEYFDEATKRKYVPHVIEPSLGVDRLFLALLTSAYHEDE 382
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BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A6U1N9R4_9STRA (Glycine--tRNA ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1N9R4_9STRA)

HSP 1 Score: 158 bits (400), Expect = 2.040e-44
Identity = 73/90 (81.11%), Postives = 82/90 (91.11%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            +VHADD LAHYARACTDITFKFPFGESELQG+AARGCYDLTQHQE SGKS+EY  E +K K++PHVIEPSLGVDRL LA++ SAYH+DEV
Sbjct:  294 EVHADDKLAHYARACTDITFKFPFGESELQGIAARGCYDLTQHQEGSGKSLEYQVEGTKDKFLPHVIEPSLGVDRLLLALICSAYHKDEV 383
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BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7R9YDS6_9STRA (Glycine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YDS6_9STRA)

HSP 1 Score: 156 bits (395), Expect = 2.130e-43
Identity = 73/91 (80.22%), Postives = 80/91 (87.91%), Query Frame = 1 
Query:    1 RDVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            +DVH D  LAHYARACTDITFKFPFGESELQG+AARG YDL  HQ SSGKS EYFDE +K KYVPHVIEPS+GV+RLFLA++ SAYHEDEV
Sbjct:  328 KDVHEDCDLAHYARACTDITFKFPFGESELQGIAARGDYDLNAHQGSSGKSQEYFDEANKRKYVPHVIEPSIGVERLFLALVCSAYHEDEV 418
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BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7S3AL73_9EUKA (Glycine--tRNA ligase n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3AL73_9EUKA)

HSP 1 Score: 146 bits (369), Expect = 3.610e-42
Identity = 68/90 (75.56%), Postives = 75/90 (83.33%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            DVH    LAHYARACTDI FKFPFG  ELQGVAARGC+DLTQH  +SGKSMEY+D+  K KYVPHVIEPS+GVDRL LA++ SAY EDEV
Sbjct:   42 DVHPSHKLAHYARACTDIMFKFPFGTQELQGVAARGCFDLTQHAGASGKSMEYYDDVRKEKYVPHVIEPSIGVDRLMLAVLCSAYKEDEV 131
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7S0CDM5_9STRA (Glycine--tRNA ligase (Fragment) n=1 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0CDM5_9STRA)

HSP 1 Score: 145 bits (365), Expect = 6.320e-42
Identity = 64/91 (70.33%), Postives = 76/91 (83.52%), Query Frame = 1 
Query:    1 RDVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            RD+H  DGLAHYARACTDI F+FPFG  EL G+AARG YDLTQH E SGK+++YFDE +K +Y+PHVIEPSLGVDRL LA++ SAY ED +
Sbjct:  104 RDIHLGDGLAHYARACTDIEFRFPFGTQELMGIAARGNYDLTQHSEGSGKTLDYFDEVTKERYIPHVIEPSLGVDRLVLALICSAYREDTI 194
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A1G3Z6K9_9BACT (Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacterium GWC2_42_7 TaxID=1802430 RepID=A0A1G3Z6K9_9BACT)

HSP 1 Score: 152 bits (383), Expect = 6.810e-42
Identity = 66/90 (73.33%), Postives = 80/90 (88.89%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            DVH  + LAHY+RACTDITFK+PFG  EL+G+AARGC+DLTQHQE+SGKS+EYFDE  K KY+PHVIEP++GVDR+FLAI+ SAY EDE+
Sbjct:  302 DVHPKNKLAHYSRACTDITFKYPFGVQELEGIAARGCFDLTQHQEASGKSLEYFDEEKKAKYLPHVIEPAVGVDRIFLAILCSAYDEDEI 391
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7S0I0N9_9EUKA (Glycine--tRNA ligase n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0I0N9_9EUKA)

HSP 1 Score: 144 bits (364), Expect = 1.290e-41
Identity = 68/90 (75.56%), Postives = 76/90 (84.44%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            DVHA D LAHYA+ACTDI F+FPFG+ ELQGVAARG YDLTQH  +SGKSMEYFD+  K KYVPHVIEPS+GVDRL LA++ SAY  DEV
Sbjct:   24 DVHAKDKLAHYAQACTDIEFQFPFGKQELQGVAARGNYDLTQHAAASGKSMEYFDDALKEKYVPHVIEPSIGVDRLMLAVLTSAYCTDEV 113
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7R9WRX3_9STRA (Glycine--tRNA ligase n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WRX3_9STRA)

HSP 1 Score: 145 bits (366), Expect = 3.290e-41
Identity = 64/90 (71.11%), Postives = 77/90 (85.56%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            DVH  D LAHYARACTD+TF+FPFGE EL G+AARG +DLTQH E SGKS+++FD+++K KY+PH IEPSLGVDRL LA++ SAY EDEV
Sbjct:   85 DVHEGDSLAHYARACTDVTFRFPFGEQELMGIAARGNFDLTQHSEGSGKSLDFFDDQTKEKYIPHCIEPSLGVDRLMLALICSAYAEDEV 174
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: W7TM99_9STRA (Glycine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TM99_9STRA)

HSP 1 Score: 150 bits (380), Expect = 4.910e-41
Identity = 69/91 (75.82%), Postives = 79/91 (86.81%), Query Frame = 1 
Query:    1 RDVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            RDVH    LAHYA+ACTDI F+FPFG+ ELQG+AARG YDLTQHQ SSGKSMEYFDE +K +YVPHVIEPS+GVDRLFLA++ SAY EDE+
Sbjct:  380 RDVHLKGDLAHYAKACTDIVFRFPFGQVELQGIAARGDYDLTQHQTSSGKSMEYFDEANKRRYVPHVIEPSIGVDRLFLALICSAYAEDEI 470
[Close]
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Match: A0A7S4IYG8_9EUKA (Glycine--tRNA ligase n=1 Tax=Prymnesium polylepis TaxID=72548 RepID=A0A7S4IYG8_9EUKA)

HSP 1 Score: 147 bits (371), Expect = 5.600e-41
Identity = 68/90 (75.56%), Postives = 76/90 (84.44%), Query Frame = 1 
Query:    4 DVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGKSMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV 273
            DVHAD  LAHYARACTDI FKFPFG  ELQGVAARGC+DL+ H ++SGKSMEY+DE  K KYVPHVIEPS+GVDRL LA++ SAY  DEV
Sbjct:  188 DVHADHKLAHYARACTDIMFKFPFGTQELQGVAARGCFDLSAHADASGKSMEYYDEERKEKYVPHVIEPSIGVDRLMLAVLTSAYCIDEV 277
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig10371.533.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YRJ7_9STRA1.220e-4583.33Glycine--tRNA ligase n=1 Tax=Tribonema minus TaxID... [more]
A0A6U1N9R4_9STRA2.040e-4481.11Glycine--tRNA ligase n=1 Tax=Fibrocapsa japonica T... [more]
A0A7R9YDS6_9STRA2.130e-4380.22Glycine--tRNA ligase n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A7S3AL73_9EUKA3.610e-4275.56Glycine--tRNA ligase n=1 Tax=Haptolina ericina Tax... [more]
A0A7S0CDM5_9STRA6.320e-4270.33Glycine--tRNA ligase (Fragment) n=1 Tax=Proboscia ... [more]
A0A1G3Z6K9_9BACT6.810e-4273.33Glycine--tRNA ligase n=1 Tax=Verrucomicrobia bacte... [more]
A0A7S0I0N9_9EUKA1.290e-4175.56Glycine--tRNA ligase n=1 Tax=Phaeocystis antarctic... [more]
A0A7R9WRX3_9STRA3.290e-4171.11Glycine--tRNA ligase n=1 Tax=Craspedostauros austr... [more]
W7TM99_9STRA4.910e-4175.82Glycine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID... [more]
A0A7S4IYG8_9EUKA5.600e-4175.56Glycine--tRNA ligase n=1 Tax=Prymnesium polylepis ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig10371contigS-firma_F_contig10371:3732..4732 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog382464.ABSI01000011_gene3153
PFAMsHGTP_anticodon,tRNA-synt_2b
Max annot lvl203494|Verrucomicrobiae
KEGG rclassRC00055,RC00523
KEGG koko:K01880
KEGG ReactionR03654
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Evalue1.22e-39
EggNOG OGsCOG0423@1|root,COG0423@2|Bacteria,46TEV@74201|Verrucomicrobia,2ITMG@203494|Verrucomicrobiae
EC6.1.1.14
DescriptionAnticodon binding domain
COG categoryJ
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016,ko03029
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionGlycyl-tRNA Synthetase (C-terminal region)
Ec32 orthologEc-07_006780.1
Exons2
Model size273
Cds size273
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815569.5911806-CDS-S-firma_F_contig10371:3731..38241622815569.5911806-CDS-S-firma_F_contig10371:3731..3824Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10371 3732..3824 +
1696949423.0270956-CDS-S-firma_F_contig10371:3731..38241696949423.0270956-CDS-S-firma_F_contig10371:3731..3824Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10371 3732..3824 +
1622815569.602595-CDS-S-firma_F_contig10371:4552..47321622815569.602595-CDS-S-firma_F_contig10371:4552..4732Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10371 4553..4732 +
1696949423.0671113-CDS-S-firma_F_contig10371:4552..47321696949423.0671113-CDS-S-firma_F_contig10371:4552..4732Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10371 4553..4732 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig10371.533.1prot_S-firma_F_contig10371.533.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig10371 3732..4732 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig10371.533.1

>prot_S-firma_F_contig10371.533.1 ID=prot_S-firma_F_contig10371.533.1|Name=mRNA_S-firma_F_contig10371.533.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=91bp
RDVHADDGLAHYARACTDITFKFPFGESELQGVAARGCYDLTQHQESSGK
SMEYFDERSKTKYVPHVIEPSLGVDRLFLAIMVSAYHEDEV
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mRNA from alignment at S-firma_F_contig10371:3732..4732+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig10371.533.1 ID=mRNA_S-firma_F_contig10371.533.1|Name=mRNA_S-firma_F_contig10371.533.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=1001bp|location=Sequence derived from alignment at S-firma_F_contig10371:3732..4732+ (Sphaerotrichia firma ET2_F female)
AGGGATGTGCACGCAGACGACGGGCTCGCGCACTACGCAAGGGCGTGCAC
CGACATTACGTTCAAGTTCCCGTTCGGGGAGTCGGAGCTGCAGGTGGGGC
GGAGTTATGTTAAAATACAACAGCATTGTTGGAGACTTTTTTTTTCTTTG
TGGGAACGAATCCGTGTGCTTTGTACGGTTTTTTTGTCGCAACCGACGGC
GCGGTCGGTGGGAGGTGGAGTATCATGTTTGTTGTCAGCACTTTCGCCAT
GGTTACTTTAGATGAATTTCTACGTACGTACAGTATTGATAGTGTCGTTT
CACTTGAACGCCGTATACTCTTAATACTGCTGTAACCCTGTACTATCAAT
ACTACTGTGAGCGTATATTAATGCTACTGTAAGCCTGCTGCCTGCCGCCG
TGTCTATTAAACAGTACTATCAATACTACCGTGAGATTATACTTCGAAGT
ATTAATACTACTACGTAAGCCTGCTGCCTGCCGCCTTGTATATTAAACAC
CATCACGATTTGCTATTAAACACCATCACGATCGGCATTAGTACTACTGC
TGTAAGCCTGCTGCCTGCTGTCTTGTCTATTAATGTTAAATTGTCTAGAA
GTATTAATATTAAATTGTCTTAATACGGAGTATTAATTTGTCTATTAAAA
TTAAACACCACCACGATCGGCCGTTGACCTTTGACATCGACATCGCGTGC
ACATCTTAAACACGTCCTGCGGTGACGAGTTCATGTACATGTACATAATA
TGACACGACATGACATGACATGACACATGACATGACATAACATAACATAA
CATGACACGCTGGCATTCCAGGGCGTGGCCGCGCGAGGCTGCTACGACCT
GACCCAGCACCAGGAGTCGAGCGGGAAGTCGATGGAGTACTTCGACGAGA
GGAGCAAGACCAAGTACGTCCCGCACGTCATCGAGCCGTCGCTGGGGGTG
GACCGGCTTTTCCTGGCCATCATGGTGTCTGCCTACCACGAGGATGAGGT
T
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Coding sequence (CDS) from alignment at S-firma_F_contig10371:3732..4732+

>mRNA_S-firma_F_contig10371.533.1 ID=mRNA_S-firma_F_contig10371.533.1|Name=mRNA_S-firma_F_contig10371.533.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=546bp|location=Sequence derived from alignment at S-firma_F_contig10371:3732..4732+ (Sphaerotrichia firma ET2_F female)
AGGGATGTGCACGCAGACGACGGGCTCGCGCACTACGCAAGGGCGTGCAC
CGACATTACGTTCAAGTTCCCGTTCGGGGAGTCGGAGCTGCAGAGGGATG
TGCACGCAGACGACGGGCTCGCGCACTACGCAAGGGCGTGCACCGACATT
ACGTTCAAGTTCCCGTTCGGGGAGTCGGAGCTGCAGGGCGTGGCCGCGCG
AGGCTGCTACGACCTGACCCAGCACCAGGAGTCGAGCGGGAAGTCGATGG
AGTACTTCGACGAGAGGAGCAAGACCAAGTACGTCCCGCACGTCATCGAG
CCGTCGCTGGGGGTGGACCGGCTTTTCCTGGCCATCATGGTGTCTGCCTA
CCACGAGGATGAGGTTGGCGTGGCCGCGCGAGGCTGCTACGACCTGACCC
AGCACCAGGAGTCGAGCGGGAAGTCGATGGAGTACTTCGACGAGAGGAGC
AAGACCAAGTACGTCCCGCACGTCATCGAGCCGTCGCTGGGGGTGGACCG
GCTTTTCCTGGCCATCATGGTGTCTGCCTACCACGAGGATGAGGTT
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