mRNA_S-firma_M_contig9394.20373.1 (mRNA) Sphaerotrichia firma Sfir_13m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_M_contig9394.20373.1
Unique NamemRNA_S-firma_M_contig9394.20373.1
TypemRNA
OrganismSphaerotrichia firma Sfir_13m male (Sphaerotrichia firma Sfir_13m male)
Homology
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A6H5K4A6_9PHAE (Helicase ATP-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K4A6_9PHAE)

HSP 1 Score: 187 bits (475), Expect = 3.260e-53
Identity = 93/98 (94.90%), Postives = 95/98 (96.94%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            F DFRMRRSL KVVKIRRDRASLPIAA+QD IVRAVAS+PCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA
Sbjct:  382 FIDFRMRRSLTKVVKIRRDRASLPIAAFQDAIVRAVASNPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 479
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: D8LEZ4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEZ4_ECTSI)

HSP 1 Score: 187 bits (474), Expect = 6.070e-53
Identity = 93/98 (94.90%), Postives = 96/98 (97.96%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            F DFRMRRSLAKVVKIRRDRASLPIAA+Q+ IVRAVAS+PCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA
Sbjct:  347 FIDFRMRRSLAKVVKIRRDRASLPIAAFQNAIVRAVASNPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 444
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A7S0PS31_MICPS (RNA helicase (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0PS31_MICPS)

HSP 1 Score: 140 bits (352), Expect = 1.690e-36
Identity = 67/96 (69.79%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    7 DFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            DFR RRSLAK+VK+ +D+A LPIAA  D IVR+V  HP  L+AGDTGCGKSTQVPQYLL+AG+ RVACTQPRR++A  L RRVS+ETL+E G E+A
Sbjct:  151 DFRQRRSLAKLVKLAKDKAQLPIAAEMDAIVRSVTEHPVTLIAGDTGCGKSTQVPQYLLRAGFNRVACTQPRRVAATALARRVSHETLNEWGGEIA 246
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: UPI001C66DFB4 (probable ATP-dependent RNA helicase DHX34 n=1 Tax=Lagopus leucura TaxID=30410 RepID=UPI001C66DFB4)

HSP 1 Score: 131 bits (329), Expect = 2.600e-36
Identity = 61/96 (63.54%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSE 288
            + DF  ++S +K+ KIRRDRA+LPIA +++ I+RAVA H  VLVAGDTGCGKSTQVPQ+LL+AGY  VACTQPRRI+ + L +RV+YE+L +HGS+
Sbjct:  122 YLDFTQKQSFSKLAKIRRDRAALPIARFREPILRAVARHRVVLVAGDTGCGKSTQVPQFLLEAGYQNVACTQPRRIACIALAKRVAYESLQQHGSQ 217
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A7S0IG38_MICPS (Hypothetical protein n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0IG38_MICPS)

HSP 1 Score: 138 bits (347), Expect = 7.700e-36
Identity = 66/96 (68.75%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    7 DFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            DFR RRSLAK+VK+ +D++ LPIAA  D IVR+V  HP  L+AGDTGCGKSTQVPQYLL+AG+ RVACTQPRR++A  L RRVS+ETL+E G E+A
Sbjct:  150 DFRQRRSLAKLVKLAKDKSKLPIAAEMDAIVRSVREHPVTLIAGDTGCGKSTQVPQYLLRAGFDRVACTQPRRVAATALARRVSHETLNEWGGEIA 245
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A226MC08_CALSU (Uncharacterized protein (Fragment) n=1 Tax=Callipepla squamata TaxID=9009 RepID=A0A226MC08_CALSU)

HSP 1 Score: 128 bits (321), Expect = 4.420e-35
Identity = 59/94 (62.77%), Postives = 78/94 (82.98%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHG 282
            + DF  +RS +K+ KIRRDR++LPIA Y++ I+ AVA H  V+VAGDTGCGKSTQVPQ+LL+AGYG +ACTQPRRI+ + L +RV+YE+L +HG
Sbjct:  124 YLDFAQKRSFSKLAKIRRDRSALPIARYREAILSAVARHRVVVVAGDTGCGKSTQVPQFLLEAGYGGLACTQPRRIACIALAKRVAYESLGQHG 217
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: C1EBK8_MICCC (RNA helicase n=1 Tax=Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) TaxID=296587 RepID=C1EBK8_MICCC)

HSP 1 Score: 134 bits (337), Expect = 1.790e-34
Identity = 65/96 (67.71%), Postives = 79/96 (82.29%), Query Frame = 1 
Query:    7 DFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            DFR RRSLAK+VK+ +D++ LPIAA  D IVR+V  HP  L+AGDTGCGKSTQVPQYLL+AG+ RVA TQPRR++A  L RRVS+ETL+E G E+A
Sbjct:  195 DFRQRRSLAKLVKLAKDKSKLPIAAEMDAIVRSVTEHPVTLIAGDTGCGKSTQVPQYLLRAGFRRVALTQPRRVAATALARRVSHETLNEWGGEIA 290
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A7S3XJB8_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XJB8_HETAK)

HSP 1 Score: 122 bits (307), Expect = 3.630e-34
Identity = 58/98 (59.18%), Postives = 76/98 (77.55%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            + DF +R   A V K++R +  LP+A  +  ++RAVA + CV+VAGDTGCGKSTQVPQYLL+ G+ R+ACTQPRR+SA+ LCRRV+ ETL E+G EVA
Sbjct:   15 YLDFALRARFAAVAKLKRTQRDLPMATARARLLRAVAENQCVVVAGDTGCGKSTQVPQYLLRTGHTRIACTQPRRLSAIALCRRVAAETLDEYGDEVA 112
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: A0A8J9WKD3_9CHLO (Probable ATP-dependent RNA helicase DHX34 n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9WKD3_9CHLO)

HSP 1 Score: 132 bits (332), Expect = 8.460e-34
Identity = 61/96 (63.54%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:    7 DFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            DF  +R L K+ K+ +D+A+LPIAAY+  IV AV ++P V+VAGDTGCGKSTQ+PQ+LL++GY  VACTQPRRISA+ LCRRV++ETLHEH  ++A
Sbjct:  131 DFHQKRQLQKLRKLLQDKAALPIAAYETRIVEAVRANPAVVVAGDTGCGKSTQLPQFLLKSGYSHVACTQPRRISAIALCRRVAFETLHEHRDDIA 226
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BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Match: UPI000B3DA88B (probable ATP-dependent RNA helicase DHX34 n=1 Tax=Numida meleagris TaxID=8996 RepID=UPI000B3DA88B)

HSP 1 Score: 132 bits (331), Expect = 1.120e-33
Identity = 62/98 (63.27%), Postives = 81/98 (82.65%), Query Frame = 1 
Query:    1 FFDFRMRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQVPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA 294
            + DF  ++S AK+ KIRRDRA+LPIA +++ I+RAVA H  VLVAGDTGCGKSTQVPQ+LL+AGY  VACTQPRRI+ + L +RV+YE+L +HG +V+
Sbjct:  148 YLDFVQKQSFAKLAKIRRDRAALPIARFREPILRAVARHRVVLVAGDTGCGKSTQVPQFLLEAGYRNVACTQPRRIACVALAKRVAYESLQQHGDQVS 245
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_M_contig9394.20373.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K4A6_9PHAE3.260e-5394.90Helicase ATP-binding domain-containing protein n=1... [more]
D8LEZ4_ECTSI6.070e-5394.90Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S0PS31_MICPS1.690e-3669.79RNA helicase (Fragment) n=1 Tax=Micromonas pusilla... [more]
UPI001C66DFB42.600e-3663.54probable ATP-dependent RNA helicase DHX34 n=1 Tax=... [more]
A0A7S0IG38_MICPS7.700e-3668.75Hypothetical protein n=1 Tax=Micromonas pusilla Ta... [more]
A0A226MC08_CALSU4.420e-3562.77Uncharacterized protein (Fragment) n=1 Tax=Callipe... [more]
C1EBK8_MICCC1.790e-3467.71RNA helicase n=1 Tax=Micromonas commoda (strain RC... [more]
A0A7S3XJB8_HETAK3.630e-3459.18Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A8J9WKD3_9CHLO8.460e-3463.54Probable ATP-dependent RNA helicase DHX34 n=1 Tax=... [more]
UPI000B3DA88B1.120e-3363.27probable ATP-dependent RNA helicase DHX34 n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_M_contig9394contigS-firma_M_contig9394:2751..3663 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma male vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma Sfir_13m male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score179.1
Seed ortholog evalue9.5e-43
Seed eggNOG ortholog2880.D8LEZ4
Preferred nameDHX34
KEGG koko:K20101
GOsGO:0000956,GO:0003674,GO:0003676,GO:0003723,GO:0003724,GO:0003824,GO:0004004,GO:0004386,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006139,GO:0006396,GO:0006401,GO:0006402,GO:0006725,GO:0006807,GO:0008026,GO:0008150,GO:0008152,GO:0008186,GO:0009056,GO:0009057,GO:0009892,GO:0009893,GO:0009894,GO:0009895,GO:0009987,GO:0010467,GO:0010468,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0016070,GO:0016071,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019219,GO:0019222,GO:0019439,GO:0031323,GO:0031324,GO:0031329,GO:0031330,GO:0034641,GO:0034655,GO:0042623,GO:0043170,GO:0044237,GO:0044238,GO:0044248,GO:0044260,GO:0044265,GO:0044270,GO:0044424,GO:0044464,GO:0045934,GO:0046483,GO:0046700,GO:0048518,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0051171,GO:0051172,GO:0051252,GO:0051253,GO:0060255,GO:0061013,GO:0065007,GO:0070035,GO:0071704,GO:0080090,GO:0090304,GO:0097159,GO:0140098,GO:1901360,GO:1901361,GO:1901363,GO:1901575,GO:1902369,GO:1902373,GO:1903311,GO:1903312,GO:2000622,GO:2000623
EggNOG free text desc.helicase activity
EggNOG OGsCOG1643@1,KOG0922@2759
EC3.6.4.13
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03019
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionHelicase, C-terminal
Ec32 orthologEc-21_006120.1
Exons2
Model size294
Cds size279
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_M_contig9394.20373.1prot_S-firma_M_contig9394.20373.1Sphaerotrichia firma Sfir_13m malepolypeptideS-firma_M_contig9394 2766..3663 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622828771.5331244-UTR-S-firma_M_contig9394:2750..27651622828771.5331244-UTR-S-firma_M_contig9394:2750..2765Sphaerotrichia firma Sfir_13m maleUTRS-firma_M_contig9394 2751..2765 +
1697105152.6877458-UTR-S-firma_M_contig9394:2750..27651697105152.6877458-UTR-S-firma_M_contig9394:2750..2765Sphaerotrichia firma Sfir_13m maleUTRS-firma_M_contig9394 2751..2765 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622828771.545788-CDS-S-firma_M_contig9394:2765..29151622828771.545788-CDS-S-firma_M_contig9394:2765..2915Sphaerotrichia firma Sfir_13m maleCDSS-firma_M_contig9394 2766..2915 +
1697105152.7002883-CDS-S-firma_M_contig9394:2765..29151697105152.7002883-CDS-S-firma_M_contig9394:2765..2915Sphaerotrichia firma Sfir_13m maleCDSS-firma_M_contig9394 2766..2915 +
1622828771.5578074-CDS-S-firma_M_contig9394:3534..36631622828771.5578074-CDS-S-firma_M_contig9394:3534..3663Sphaerotrichia firma Sfir_13m maleCDSS-firma_M_contig9394 3535..3663 +
1697105152.7105892-CDS-S-firma_M_contig9394:3534..36631697105152.7105892-CDS-S-firma_M_contig9394:3534..3663Sphaerotrichia firma Sfir_13m maleCDSS-firma_M_contig9394 3535..3663 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_M_contig9394.20373.1

>prot_S-firma_M_contig9394.20373.1 ID=prot_S-firma_M_contig9394.20373.1|Name=mRNA_S-firma_M_contig9394.20373.1|organism=Sphaerotrichia firma Sfir_13m male|type=polypeptide|length=93bp
MRRSLAKVVKIRRDRASLPIAAYQDTIVRAVASHPCVLVAGDTGCGKSTQ
VPQYLLQAGYGRVACTQPRRISAMGLCRRVSYETLHEHGSEVA
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mRNA from alignment at S-firma_M_contig9394:2751..3663+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_M_contig9394.20373.1 ID=mRNA_S-firma_M_contig9394.20373.1|Name=mRNA_S-firma_M_contig9394.20373.1|organism=Sphaerotrichia firma Sfir_13m male|type=mRNA|length=913bp|location=Sequence derived from alignment at S-firma_M_contig9394:2751..3663+ (Sphaerotrichia firma Sfir_13m male)
TTCTTCGACTTCCGCATGCGGCGAAGCTTGGCTAAGGTGGTCAAAATCCG
GCGCGACCGCGCTTCCCTGCCGATTGCAGCCTACCAGGACACGATCGTGA
GAGCCGTCGCATCCCATCCGTGCGTGCTCGTGGCTGGAGACACCGGCTGC
GGCAAGAGCACGCAGGTAAAGTACGAGGTTAGCGGGCTTAATTAACCTGA
TAAATCATGTCCGCGGGATCTGGAAACGGGCTGTAAAAGGTTCGGTCGTT
GGAGTGGTTGCGTCAGGGAGGGTAAAGGCGTAGCTTGTGCGTGGAGTTGG
GCGTTTTGTGTGAGCGCGCGTGCACGTTTTGTGTGTGTTGCTGAAGAACG
GCGTCCATCCTCGTAGCTGCCTGCTCAATCTGCCAGCAGCAGCACCGGGG
AGTACCGCAAGGTACAGCCTGTTGCGCTCGTTTCATAAGGAAGACTGGAT
GTACGCCTGCTACTCATGCTCAACCATTCTTCAACGGGCAGCAATTAGGT
ACTCGAGCACCATGATGTGTGCGTTCTACGAAGATGCACGTGTTCTCTTT
AGTTTTGCTCCGTTGTCCGGCCGAATACGCTCGTGGCGGGAGCGACACCC
ACCTCCGCCGCCGCTGCTGCTGCTGCTGCTGCTGGAGAATCGACCCCCCA
AGTGCGACATCGTTGTCGCTGTCGCGGTCGTTTTCGGGGGCACGTGGGAG
GAGCTTGCTGTCCAGTAGAGCAACGTGAATCAATCAACCGGCGTCGCGCA
AAAAATTAAAAAAAAAAACATCTGCCGTCTGCAGGTGCCGCAGTACCTGC
TGCAGGCGGGCTACGGCAGGGTTGCCTGCACCCAGCCCAGGAGGATTTCC
GCCATGGGTCTCTGCCGCAGAGTGTCGTATGAGACGCTCCATGAGCACGG
CTCCGAGGTGGCG
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Coding sequence (CDS) from alignment at S-firma_M_contig9394:2751..3663+

>mRNA_S-firma_M_contig9394.20373.1 ID=mRNA_S-firma_M_contig9394.20373.1|Name=mRNA_S-firma_M_contig9394.20373.1|organism=Sphaerotrichia firma Sfir_13m male|type=CDS|length=558bp|location=Sequence derived from alignment at S-firma_M_contig9394:2751..3663+ (Sphaerotrichia firma Sfir_13m male)
ATGCGGCGAAGCTTGGCTAAGGTGGTCAAAATCCGGCGCGACCGCGCTTC
CCTGCCGATTGCAGCCTACCAGGACACGATCGTGAGAGCCGTCGCATCCC
ATCCGTGCGTGCTCGTGGCTGGAGACACCGGCTGCGGCAAGAGCACGCAG
ATGCGGCGAAGCTTGGCTAAGGTGGTCAAAATCCGGCGCGACCGCGCTTC
CCTGCCGATTGCAGCCTACCAGGACACGATCGTGAGAGCCGTCGCATCCC
ATCCGTGCGTGCTCGTGGCTGGAGACACCGGCTGCGGCAAGAGCACGCAG
GTGCCGCAGTACCTGCTGCAGGCGGGCTACGGCAGGGTTGCCTGCACCCA
GCCCAGGAGGATTTCCGCCATGGGTCTCTGCCGCAGAGTGTCGTATGAGA
CGCTCCATGAGCACGGCTCCGAGGTGGCGGTGCCGCAGTACCTGCTGCAG
GCGGGCTACGGCAGGGTTGCCTGCACCCAGCCCAGGAGGATTTCCGCCAT
GGGTCTCTGCCGCAGAGTGTCGTATGAGACGCTCCATGAGCACGGCTCCG
AGGTGGCG
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