mRNA_S-firma_F_contig10519.729.1 (mRNA) Sphaerotrichia firma ET2_F female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_S-firma_F_contig10519.729.1
Unique NamemRNA_S-firma_F_contig10519.729.1
TypemRNA
OrganismSphaerotrichia firma ET2_F female (Sphaerotrichia firma ET2_F female)
Homology
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: D7FQB2_ECTSI (Similar to ATP binding / alanine-tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQB2_ECTSI)

HSP 1 Score: 114 bits (286), Expect = 7.490e-26
Identity = 71/126 (56.35%), Postives = 83/126 (65.87%), Query Frame = 2 
Query:   20 NTELDKLVMADMATVVETGQDSNGGKAPAPL-----EGEAVGVGGTENK----VPSHGGVERRAGAEGIS---GATKYGAETRIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSL 361
            N+ELDKLVMA+M T+V+     +G +AP+       EG + G  G   +    V   GG   R      S    ATKYGA TR VTVAGLSCPCGGTHVRSTRE+EGVR+TKVKAKKGTLRVSY+L
Sbjct:  157 NSELDKLVMANMETMVKMADQEHGDEAPSSSSSSAQEGPSRGAAGRGREEVTVVAERGGRGGRGKVPASSTAAAATKYGAATRTVTVAGLSCPCGGTHVRSTREMEGVRITKVKAKKGTLRVSYTL 282
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A6H5JSV6_9PHAE (tRNA_SAD domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSV6_9PHAE)

HSP 1 Score: 98.6 bits (244), Expect = 2.140e-21
Identity = 59/105 (56.19%), Postives = 69/105 (65.71%), Query Frame = 2 
Query:   68 ETGQDSNGGKAPAPLEGEAVGVGGT-ENKVPSHGGVERRAGAEGI------SGATKYGAETRIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSL 361
            E G ++    A +  EG A G  GT   +V      E R G   +      + ATKYGA TRIVTVAGLSCPCGGTHVRSTRE+EGVR+TK KAKKGT+RVSY+L
Sbjct:   22 EHGDEAPSSSASSAQEGPARGAAGTGREEVAVVAEPEGRRGKGNVPASLTAAAATKYGAATRIVTVAGLSCPCGGTHVRSTREMEGVRITKAKAKKGTIRVSYTL 126
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A835Z9X7_9STRA (Alanyl-tRNA editing protein AlaX-M-like protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9X7_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 4.890e-10
Identity = 31/44 (70.45%), Postives = 37/44 (84.09%), Query Frame = 2 
Query:  230 RIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSL 361
            R+V+V G +CPCGGTHVRST EL G+RVTK KAK G LRVSY++
Sbjct:  179 RVVSVGGCACPCGGTHVRSTAELRGLRVTKAKAKSGRLRVSYTI 222
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A7S1TVS7_9STRA (Hypothetical protein n=3 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TVS7_9STRA)

HSP 1 Score: 67.8 bits (164), Expect = 3.510e-9
Identity = 34/48 (70.83%), Postives = 40/48 (83.33%), Query Frame = 2 
Query:  230 RIVTVAGL-SCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSLISP 370
            R+VTVAG  +CPCGGTHVRST EL GV VTKVK+KKG ++VSYS+  P
Sbjct:  236 RVVTVAGGGTCPCGGTHVRSTAELGGVTVTKVKSKKGRIKVSYSVTPP 283
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A7S2WSC3_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WSC3_9STRA)

HSP 1 Score: 62.4 bits (150), Expect = 2.600e-7
Identity = 29/48 (60.42%), Postives = 36/48 (75.00%), Query Frame = 2 
Query:  218 GAETRIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSL 361
            GA  R+V V G  CPCGGTHV +  ELE V VT++KAKKGT++VSY +
Sbjct:  245 GAMIRVVNVGGCWCPCGGTHVGNASELERVTVTRIKAKKGTVKVSYEV 292
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A4D9CZR6_9STRA (AA_TRNA_LIGASE_II_ALA domain-containing protein n=4 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CZR6_9STRA)

HSP 1 Score: 61.2 bits (147), Expect = 5.590e-7
Identity = 25/47 (53.19%), Postives = 36/47 (76.60%), Query Frame = 2 
Query:  221 AETRIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSL 361
            A+ R+V+V G  CPCGGTHV+ST+EL GV +T++K KK   ++SY +
Sbjct:  227 AQVRLVSVGGNICPCGGTHVKSTKELGGVEITRIKTKKNVTKISYRI 273
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A7S1DXT9_9STRA (Hypothetical protein n=1 Tax=Thalassionema nitzschioides TaxID=33649 RepID=A0A7S1DXT9_9STRA)

HSP 1 Score: 56.6 bits (135), Expect = 1.290e-6
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = 2 
Query:  212 KYGAET-RIVTVAGLSCPCGGTHVRSTRELEGVR--VTKVKAKKGTLRVSYS 358
            +YG ++ RIVTVAG +CPCGGTHVRST++L+  +  V  +K+KKG +RV Y+
Sbjct:   46 EYGDDSIRIVTVAGWACPCGGTHVRSTKDLKQRQWGVVGIKSKKGVVRVKYA 97
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A7G7HSS4_9MICO (tRNA_SAD domain-containing protein n=1 Tax=Janibacter sp. YB324 TaxID=2761047 RepID=A0A7G7HSS4_9MICO)

HSP 1 Score: 56.6 bits (135), Expect = 1.380e-6
Identity = 29/62 (46.77%), Postives = 43/62 (69.35%), Query Frame = 2 
Query:  191 EGISGATKYGAET-----RIVTVAGLSC-PCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYS 358
            E ++ A  +GAE      R VT+ GL   PCGGTHVR+T +L G+++T+V+ KKG L++SY+
Sbjct:   41 EHVAAALPFGAEVDAEGVRRVTIEGLQTDPCGGTHVRTTADLTGIQITRVRTKKGQLKLSYT 102
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: L8GVN5_ACACA (Threonine/alanine tRNA ligase subfamily protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GVN5_ACACA)

HSP 1 Score: 58.5 bits (140), Expect = 3.870e-6
Identity = 32/67 (47.76%), Postives = 45/67 (67.16%), Query Frame = 2 
Query:  170 VERRAGAEGISGATKYGAETRIVTV-AGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYSLIS 367
            VER  G+   SGA       R+VTV A L CPCGGTHV++T E+  + + K+ AKKG +R+SY+L++
Sbjct:  199 VERLCGSASDSGANPT-EPVRVVTVCAPLGCPCGGTHVQNTGEIGRIAIPKMSAKKGVVRISYTLLA 264
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BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Match: A0A1W1YJ62_9MICO (Threonyl and Alanyl tRNA synthetase second additional domain-containing protein n=2 Tax=Janibacter indicus TaxID=857417 RepID=A0A1W1YJ62_9MICO)

HSP 1 Score: 55.8 bits (133), Expect = 5.480e-6
Identity = 29/62 (46.77%), Postives = 43/62 (69.35%), Query Frame = 2 
Query:  191 EGISGATKYGAET-----RIVTVAGLSC-PCGGTHVRSTRELEGVRVTKVKAKKGTLRVSYS 358
            E ++ A  +GAE      R VT+ GL   PCGGTHVR+T +L G+++T+V+ KKG L++SY+
Sbjct:   75 EHVAAALPFGAEVDAEGVRRVTIEGLQTDPCGGTHVRTTADLTGLQITRVRTKKGQLKLSYT 136
The following BLAST results are available for this feature:
BLAST of mRNA_S-firma_F_contig10519.729.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
D7FQB2_ECTSI7.490e-2656.35Similar to ATP binding / alanine-tRNA ligase n=1 T... [more]
A0A6H5JSV6_9PHAE2.140e-2156.19tRNA_SAD domain-containing protein n=1 Tax=Ectocar... [more]
A0A835Z9X7_9STRA4.890e-1070.45Alanyl-tRNA editing protein AlaX-M-like protein n=... [more]
A0A7S1TVS7_9STRA3.510e-970.83Hypothetical protein n=3 Tax=Phaeomonas parva TaxI... [more]
A0A7S2WSC3_9STRA2.600e-760.42Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A4D9CZR6_9STRA5.590e-753.19AA_TRNA_LIGASE_II_ALA domain-containing protein n=... [more]
A0A7S1DXT9_9STRA1.290e-655.77Hypothetical protein n=1 Tax=Thalassionema nitzsch... [more]
A0A7G7HSS4_9MICO1.380e-646.77tRNA_SAD domain-containing protein n=1 Tax=Janibac... [more]
L8GVN5_ACACA3.870e-647.76Threonine/alanine tRNA ligase subfamily protein n=... [more]
A0A1W1YJ62_9MICO5.480e-646.77Threonyl and Alanyl tRNA synthetase second additio... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
S-firma_F_contig10519contigS-firma_F_contig10519:2402..3747 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Sphaerotrichia firma female vs UniRef902022-09-19
OGS1.0 of Sphaerotrichia firma ET2_F female2021-02-24
Properties
Property NameValue
Seed ortholog2880.D7FQB2
PFAMstRNA-synt_2c,tRNA_SAD
Max annot lvl2759|Eukaryota
Evalue8.67e-25
EggNOG OGsCOG2872@1|root,2S23F@2759|Eukaryota
DescriptionThreonyl and Alanyl tRNA synthetase second additional domain
COG categoryS
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionSimilar to ATP binding / alanine-tRNA ligase
Ec32 orthologEc-01_006770.1
Exons3
Model size1074
Cds size333
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_S-firma_F_contig10519.729.1prot_S-firma_F_contig10519.729.1Sphaerotrichia firma ET2_F femalepolypeptideS-firma_F_contig10519 2445..3022 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815588.177917-UTR-S-firma_F_contig10519:2401..24441622815588.177917-UTR-S-firma_F_contig10519:2401..2444Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 2402..2444 +
1696949436.8045247-UTR-S-firma_F_contig10519:2401..24441696949436.8045247-UTR-S-firma_F_contig10519:2401..2444Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 2402..2444 +
1622815588.2274303-UTR-S-firma_F_contig10519:3022..34941622815588.2274303-UTR-S-firma_F_contig10519:3022..3494Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 3023..3494 +
1696949436.838396-UTR-S-firma_F_contig10519:3022..34941696949436.838396-UTR-S-firma_F_contig10519:3022..3494Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 3023..3494 +
1622815588.2473764-UTR-S-firma_F_contig10519:3521..37471622815588.2473764-UTR-S-firma_F_contig10519:3521..3747Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 3522..3747 +
1696949436.880311-UTR-S-firma_F_contig10519:3521..37471696949436.880311-UTR-S-firma_F_contig10519:3521..3747Sphaerotrichia firma ET2_F femaleUTRS-firma_F_contig10519 3522..3747 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815588.1955533-CDS-S-firma_F_contig10519:2444..24801622815588.1955533-CDS-S-firma_F_contig10519:2444..2480Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10519 2445..2480 +
1696949436.8169863-CDS-S-firma_F_contig10519:2444..24801696949436.8169863-CDS-S-firma_F_contig10519:2444..2480Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10519 2445..2480 +
1622815588.210328-CDS-S-firma_F_contig10519:2725..30221622815588.210328-CDS-S-firma_F_contig10519:2725..3022Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10519 2726..3022 +
1696949436.8262956-CDS-S-firma_F_contig10519:2725..30221696949436.8262956-CDS-S-firma_F_contig10519:2725..3022Sphaerotrichia firma ET2_F femaleCDSS-firma_F_contig10519 2726..3022 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_S-firma_F_contig10519.729.1

>prot_S-firma_F_contig10519.729.1 ID=prot_S-firma_F_contig10519.729.1|Name=mRNA_S-firma_F_contig10519.729.1|organism=Sphaerotrichia firma ET2_F female|type=polypeptide|length=111bp
MADMATVVETGQDSNGGKAPAPLEGEAVGVGGTENKVPSHGGVERRAGAE
GISGATKYGAETRIVTVAGLSCPCGGTHVRSTRELEGVRVTKVKAKKGTL
RVSYSLISPQ*
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mRNA from alignment at S-firma_F_contig10519:2402..3747+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_S-firma_F_contig10519.729.1 ID=mRNA_S-firma_F_contig10519.729.1|Name=mRNA_S-firma_F_contig10519.729.1|organism=Sphaerotrichia firma ET2_F female|type=mRNA|length=1346bp|location=Sequence derived from alignment at S-firma_F_contig10519:2402..3747+ (Sphaerotrichia firma ET2_F female)
TATTCACCTAGCGCAGTTGAACACCGAGCTCGACAAGCTCGTGATGGCGG
ACATGGCGACCGTGGTTGAGACCGGTCAGGTGAGTGGGGGCACAGAGGGA
CGATACGAACAAGGCCCGCTCTAGAATCACCTGGACGTGGCGTTTTTCTT
CCATCTCGACTACAGGTTTCAGCGTTCCTCTACACCCTCCCAAAACTGGT
GTGCTTCTAATGGGTGAAAATCAACTCCCGAAACAACGAAAACAAACAAC
TGAGCGACAGGGGTGCATTTGCGGCTGAACTCGCAAGCGCGTGTCTGCCC
GCCCTTTCCTGCGCTCAACTCCAGGACTCCAACGGCGGCAAGGCTCCAGC
GCCGCTCGAGGGCGAAGCTGTAGGAGTAGGAGGGACAGAAAACAAAGTGC
CATCACACGGAGGAGTAGAAAGGCGAGCAGGAGCGGAAGGGATATCGGGA
GCGACAAAGTATGGCGCCGAGACACGAATCGTGACGGTGGCCGGTCTCTC
CTGTCCCTGCGGCGGAACCCACGTGAGGAGCACGCGAGAACTGGAGGGCG
TGCGCGTGACGAAAGTCAAGGCCAAGAAGGGCACCCTTCGCGTCAGTTAT
TCTCTCATCTCTCCTCAATAGCAGCGGCAGCAGCAGCAGGAGCTTGTACT
TCGATATGAAAGTAGCAGACATGTTGCCAATCGCGTGTTTTCTGGACGAA
AAATCACGTATTTGTATTCGAGGCGGCACCATCTGTGTTCGTCCGTATTT
TGGTATTTTTTCGTCATTTAGGGGGTTGTTGAATTCTGTTGGCTTGGTCG
ATTGATCGAGATGGTTGGTCTCCTGCCGGTGGACTAACACGCCGTTGCGG
AGTGCGAGCCACTATCATTCGTAAAAAAACAGACTTCGGTGCGATGCTCT
CTTTTGCCTCATGCTTGGGTTCATGTGCTGGGTTTATGTACTGCGGCACA
CAAACCTGGCCAAAAGGGAGAATGGAACGCTGTACGCACGTCTACTTCGG
CGACTCGTGTCCAATGTATCATCAGCGAAATGGCGTTATTGTTGAGAAGA
CTGTTGGAGAATGTATGAGAACGACTCCAAGCAACTGGTTTGAGTTTGAT
TTGGAGGGCGTGTTTCCAAGTCTGTATTGCCGTCAGGAAGGCAGCTTGGG
CGTTTGTGTGTACCTTACTTTTGCGTTGGTTGCCCGAACTTGATGGGGCA
AGTGTTTTCACCCAGAAACCTACACAACCGCGCCTAAAAAAACAGTCGTT
CATACACAGAAGGATGTTCGTATGATCTAAGCTCGGGAGATGGAGTATGG
AACACAAATACAGCGGGCCGTCAGGTTAACGCGAACAGGACGCAAC
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Coding sequence (CDS) from alignment at S-firma_F_contig10519:2402..3747+

>mRNA_S-firma_F_contig10519.729.1 ID=mRNA_S-firma_F_contig10519.729.1|Name=mRNA_S-firma_F_contig10519.729.1|organism=Sphaerotrichia firma ET2_F female|type=CDS|length=666bp|location=Sequence derived from alignment at S-firma_F_contig10519:2402..3747+ (Sphaerotrichia firma ET2_F female)
ATGGCGGACATGGCGACCGTGGTTGAGACCGGTCAGATGGCGGACATGGC
GACCGTGGTTGAGACCGGTCAGGACTCCAACGGCGGCAAGGCTCCAGCGC
CGCTCGAGGGCGAAGCTGTAGGAGTAGGAGGGACAGAAAACAAAGTGCCA
TCACACGGAGGAGTAGAAAGGCGAGCAGGAGCGGAAGGGATATCGGGAGC
GACAAAGTATGGCGCCGAGACACGAATCGTGACGGTGGCCGGTCTCTCCT
GTCCCTGCGGCGGAACCCACGTGAGGAGCACGCGAGAACTGGAGGGCGTG
CGCGTGACGAAAGTCAAGGCCAAGAAGGGCACCCTTCGCGTCAGTTATTC
TCTCATCTCTCCTCAATAGGACTCCAACGGCGGCAAGGCTCCAGCGCCGC
TCGAGGGCGAAGCTGTAGGAGTAGGAGGGACAGAAAACAAAGTGCCATCA
CACGGAGGAGTAGAAAGGCGAGCAGGAGCGGAAGGGATATCGGGAGCGAC
AAAGTATGGCGCCGAGACACGAATCGTGACGGTGGCCGGTCTCTCCTGTC
CCTGCGGCGGAACCCACGTGAGGAGCACGCGAGAACTGGAGGGCGTGCGC
GTGACGAAAGTCAAGGCCAAGAAGGGCACCCTTCGCGTCAGTTATTCTCT
CATCTCTCCTCAATAG
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